CA2894688A1 - Crispr-cas systems and methods for altering expression of gene products in eukaryotic cells - Google Patents

Crispr-cas systems and methods for altering expression of gene products in eukaryotic cells Download PDF

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Publication number
CA2894688A1
CA2894688A1 CA2894688A CA2894688A CA2894688A1 CA 2894688 A1 CA2894688 A1 CA 2894688A1 CA 2894688 A CA2894688 A CA 2894688A CA 2894688 A CA2894688 A CA 2894688A CA 2894688 A1 CA2894688 A1 CA 2894688A1
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Prior art keywords
sequence
crispr
cell
expression
target
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CA2894688A
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English (en)
French (fr)
Inventor
Feng Zhang
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Massachusetts Institute of Technology
Broad Institute Inc
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Massachusetts Institute of Technology
Broad Institute Inc
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=50441380&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CA2894688(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Massachusetts Institute of Technology, Broad Institute Inc filed Critical Massachusetts Institute of Technology
Publication of CA2894688A1 publication Critical patent/CA2894688A1/en
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
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    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C12N9/222—Clustered regularly interspaced short palindromic repeats [CRISPR]-associated [CAS] enzymes
    • C12N9/226—Class 2 CAS enzyme complex, e.g. single CAS protein
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    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
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    • A61K38/46—Hydrolases (3)
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    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea

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  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Virology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
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CA2894688A 2012-12-12 2013-12-12 Crispr-cas systems and methods for altering expression of gene products in eukaryotic cells Pending CA2894688A1 (en)

Applications Claiming Priority (13)

Application Number Priority Date Filing Date Title
US201261736527P 2012-12-12 2012-12-12
US61/736,527 2012-12-12
US201361748427P 2013-01-02 2013-01-02
US61/748,427 2013-01-02
US201361791409P 2013-03-15 2013-03-15
US61/791,409 2013-03-15
US201361835931P 2013-06-17 2013-06-17
US61/835,931 2013-06-17
US201361842322P 2013-07-02 2013-07-02
US61/842,322 2013-07-02
US14/054,414 2013-10-15
US14/054,414 US8697359B1 (en) 2012-12-12 2013-10-15 CRISPR-Cas systems and methods for altering expression of gene products
PCT/US2013/074743 WO2014093661A2 (en) 2012-12-12 2013-12-12 Crispr-cas systems and methods for altering expression of gene products

Publications (1)

Publication Number Publication Date
CA2894688A1 true CA2894688A1 (en) 2014-06-19

Family

ID=50441380

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2894688A Pending CA2894688A1 (en) 2012-12-12 2013-12-12 Crispr-cas systems and methods for altering expression of gene products in eukaryotic cells

Country Status (15)

Country Link
US (8) US8697359B1 (https=)
EP (2) EP2998400A1 (https=)
JP (6) JP6545621B2 (https=)
KR (1) KR20150107739A (https=)
CN (4) CN106480080B (https=)
AU (5) AU2013359238B2 (https=)
BR (1) BR112015013785A2 (https=)
CA (1) CA2894688A1 (https=)
DK (1) DK2764103T3 (https=)
ES (1) ES2552535T3 (https=)
HK (1) HK1216259A1 (https=)
PL (1) PL2764103T3 (https=)
PT (1) PT2764103E (https=)
RU (1) RU2687451C1 (https=)
WO (1) WO2014093661A2 (https=)

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