AR127144A1 - Composiciones y métodos para la transformación de explantes de embriones extirpados de semillas de monocotiledóneas - Google Patents
Composiciones y métodos para la transformación de explantes de embriones extirpados de semillas de monocotiledóneasInfo
- Publication number
- AR127144A1 AR127144A1 ARP220102577A ARP220102577A AR127144A1 AR 127144 A1 AR127144 A1 AR 127144A1 AR P220102577 A ARP220102577 A AR P220102577A AR P220102577 A ARP220102577 A AR P220102577A AR 127144 A1 AR127144 A1 AR 127144A1
- Authority
- AR
- Argentina
- Prior art keywords
- explant
- plant
- embryo
- rhizobiales
- culturing
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 6
- 241000209510 Liliopsida Species 0.000 title abstract 4
- 239000000203 mixture Substances 0.000 title abstract 2
- 230000009466 transformation Effects 0.000 title abstract 2
- 210000002257 embryonic structure Anatomy 0.000 title 1
- 210000001161 mammalian embryo Anatomy 0.000 abstract 9
- 241000196324 Embryophyta Species 0.000 abstract 8
- 241000589157 Rhizobiales Species 0.000 abstract 4
- 238000012258 culturing Methods 0.000 abstract 4
- 102000040430 polynucleotide Human genes 0.000 abstract 4
- 108091033319 polynucleotide Proteins 0.000 abstract 4
- 239000002157 polynucleotide Substances 0.000 abstract 4
- 229930192334 Auxin Natural products 0.000 abstract 3
- 239000002363 auxin Substances 0.000 abstract 3
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 abstract 3
- 239000004062 cytokinin Substances 0.000 abstract 3
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 abstract 3
- 230000006698 induction Effects 0.000 abstract 3
- 239000002609 medium Substances 0.000 abstract 2
- 239000012881 co-culture medium Substances 0.000 abstract 1
- 230000001939 inductive effect Effects 0.000 abstract 1
- 239000012499 inoculation medium Substances 0.000 abstract 1
- 230000000442 meristematic effect Effects 0.000 abstract 1
- 230000002035 prolonged effect Effects 0.000 abstract 1
- 230000001172 regenerating effect Effects 0.000 abstract 1
- 230000008929 regeneration Effects 0.000 abstract 1
- 238000011069 regeneration method Methods 0.000 abstract 1
- 230000001131 transforming effect Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
- C12N15/8205—Agrobacterium mediated transformation
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/002—Culture media for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Developmental Biology & Embryology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Cell Biology (AREA)
- Biomedical Technology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
La invención proporciona composiciones y métodos novedosos para mejorar la transformación de explantes de embrión extirpados de la semilla monocotiledónea, que puede incluir una o más etapas de preparación del explante, rehidratación del explante, inoculación de y cultivo en conjunto con la bacteria Rhizobiales, la inducción de brotes, la inducción de brotes prolongada o la regeneración de plantas o partes de la planta genéticamente modificadas. Los métodos proporcionados en el presente documento pueden incluir la transformación de al menos una célula vegetal del explante de embrión con un polinucleótido heterólogo mediante la inoculación del explante de embrión con una bacteria Rhizobiales que comprende el polinucleótido heterólogo. Los métodos proporcionados en el presente documento incluyen métodos para regenerar una planta o parte de la planta genéticamente modificada a partir de una célula vegetal o explante transformado o editado. Reivindicación 1: Un método para producir una planta monocotiledónea o parte de la planta genéticamente modificada que comprende: introducir una molécula polinucleotídica heteróloga en al menos una célula de un explante de embrión de semilla monocotiledónea que comprende tejido meristemático mediante la inoculación del explante de embrión con un medio de inoculación que comprende una bacteria Rhizobiales competente para transformar dicha al menos una célula con la molécula polinucleotídica heteróloga, cultivar en conjunto el explante de embrión con la bacteria Rhizobiales en contacto con un medio de cultivo en conjunto, cultivar el explante de embrión en contacto con un primer medio de inducción de brotes que comprende una primera auxina y una primera citoquinina, cultivar el explante de embrión en contacto con un segundo medio de inducción de brotes que comprende la primera auxina o una segunda auxina y la primera citoquinina o una segunda citoquinina, y regenerar la planta monocotiledónea o parte de la planta genéticamente modificada a partir de un explante de embrión.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202163248921P | 2021-09-27 | 2021-09-27 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR127144A1 true AR127144A1 (es) | 2023-12-20 |
Family
ID=85721329
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP220102577A AR127144A1 (es) | 2021-09-27 | 2022-09-26 | Composiciones y métodos para la transformación de explantes de embriones extirpados de semillas de monocotiledóneas |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP4409007A1 (es) |
| JP (1) | JP2024536851A (es) |
| CN (1) | CN118284701A (es) |
| AR (1) | AR127144A1 (es) |
| AU (1) | AU2022349096A1 (es) |
| WO (1) | WO2023049898A1 (es) |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2126096B1 (en) * | 2007-03-09 | 2013-12-18 | Monsanto Technology, LLC | Methods for plant transformation using spectinomycin selection |
| AR116016A1 (es) * | 2018-08-24 | 2021-03-25 | Flagship Pioneering Innovations Vi Llc | Métodos para fabricar paquetes mensajeros vegetales |
| US12022787B2 (en) * | 2019-06-19 | 2024-07-02 | Wisconsin Alumni Research Foundation | Efficient monocot embryo extraction and preservation methods and novel uses thereof |
-
2022
- 2022-09-26 WO PCT/US2022/077024 patent/WO2023049898A1/en active Application Filing
- 2022-09-26 JP JP2024518721A patent/JP2024536851A/ja active Pending
- 2022-09-26 CN CN202280077272.4A patent/CN118284701A/zh active Pending
- 2022-09-26 EP EP22873918.1A patent/EP4409007A1/en active Pending
- 2022-09-26 AU AU2022349096A patent/AU2022349096A1/en active Pending
- 2022-09-26 AR ARP220102577A patent/AR127144A1/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP4409007A1 (en) | 2024-08-07 |
| WO2023049898A1 (en) | 2023-03-30 |
| CN118284701A (zh) | 2024-07-02 |
| AU2022349096A1 (en) | 2024-04-04 |
| JP2024536851A (ja) | 2024-10-08 |
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