SG11201406547YA - Nuclease-mediated targeting with large targeting vectors - Google Patents

Nuclease-mediated targeting with large targeting vectors

Info

Publication number
SG11201406547YA
SG11201406547YA SG11201406547YA SG11201406547YA SG11201406547YA SG 11201406547Y A SG11201406547Y A SG 11201406547YA SG 11201406547Y A SG11201406547Y A SG 11201406547YA SG 11201406547Y A SG11201406547Y A SG 11201406547YA SG 11201406547Y A SG11201406547Y A SG 11201406547YA
Authority
SG
Singapore
Prior art keywords
targeting
nuclease
mediated
vectors
targeting vectors
Prior art date
Application number
SG11201406547YA
Inventor
David Frendewey
Wojtek Auerbach
David M Valenzuela
George D Yancopoulos
Ka-Man Venus Lai
Original Assignee
Regeneron Pharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Regeneron Pharma filed Critical Regeneron Pharma
Publication of SG11201406547YA publication Critical patent/SG11201406547YA/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/715Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons
    • C07K14/7155Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons for interleukins [IL]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90Stable introduction of foreign DNA into chromosome
    • C12N15/902Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
SG11201406547YA 2012-04-25 2013-04-25 Nuclease-mediated targeting with large targeting vectors SG11201406547YA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261638267P 2012-04-25 2012-04-25
PCT/US2013/038165 WO2013163394A1 (en) 2012-04-25 2013-04-25 Nuclease-mediated targeting with large targeting vectors

Publications (1)

Publication Number Publication Date
SG11201406547YA true SG11201406547YA (en) 2014-11-27

Family

ID=48289700

Family Applications (2)

Application Number Title Priority Date Filing Date
SG10201702445TA SG10201702445TA (en) 2012-04-25 2013-04-25 Nuclease-mediated targeting with large targeting vectors
SG11201406547YA SG11201406547YA (en) 2012-04-25 2013-04-25 Nuclease-mediated targeting with large targeting vectors

Family Applications Before (1)

Application Number Title Priority Date Filing Date
SG10201702445TA SG10201702445TA (en) 2012-04-25 2013-04-25 Nuclease-mediated targeting with large targeting vectors

Country Status (19)

Country Link
US (2) US9834786B2 (en)
EP (1) EP2847335B1 (en)
JP (1) JP6275120B2 (en)
KR (1) KR101904508B1 (en)
CN (2) CN109536526B (en)
AU (1) AU2013251558B2 (en)
CA (1) CA2869016C (en)
CY (1) CY1120572T1 (en)
DK (1) DK2847335T3 (en)
ES (1) ES2683071T3 (en)
HK (1) HK1207396A1 (en)
IL (1) IL234905B (en)
IN (1) IN2014DN09261A (en)
MX (1) MX362523B (en)
PL (1) PL2847335T3 (en)
PT (1) PT2847335T (en)
RU (1) RU2645475C2 (en)
SG (2) SG10201702445TA (en)
WO (1) WO2013163394A1 (en)

Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG10201702445TA (en) 2012-04-25 2017-04-27 Regeneron Pharma Nuclease-mediated targeting with large targeting vectors
RS64622B1 (en) 2012-05-25 2023-10-31 Univ California Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription
KR102006880B1 (en) 2012-12-06 2019-08-02 시그마-알드리치 컴퍼니., 엘엘씨 Crispr-based genome modification and regulation
ES2904803T3 (en) 2013-02-20 2022-04-06 Regeneron Pharma Genetic modification of rats
RS58255B1 (en) 2013-04-16 2019-03-29 Regeneron Pharma Targeted modification of rat genome
EP3418379B1 (en) * 2013-09-18 2020-12-09 Kymab Limited Methods, cells & organisms
KR101566498B1 (en) * 2013-11-13 2015-11-06 건국대학교 산학협력단 Interleukin 2 receptor gamma gene targeting vector, production of SCID-like miniature pigs by TALEN-mediated gene targeting and use thereof
US10787684B2 (en) * 2013-11-19 2020-09-29 President And Fellows Of Harvard College Large gene excision and insertion
WO2015088643A1 (en) * 2013-12-11 2015-06-18 Regeneron Pharmaceuticals, Inc. Methods and compositions for the targeted modification of a genome
RU2704283C9 (en) * 2014-06-06 2020-02-07 Регенерон Фармасьютикалз, Инк. Methods and compositions for modifying target locus
ES2694629T3 (en) * 2014-06-26 2018-12-26 Regeneron Pharmaceuticals, Inc. Methods and compositions for objective genetic modifications and methods of use
ES2741387T3 (en) 2014-10-15 2020-02-10 Regeneron Pharma Methods and compositions for generating or maintaining pluripotent cells
PT3221457T (en) 2014-11-21 2019-06-27 Regeneron Pharma Methods and compositions for targeted genetic modification using paired guide rnas
KR102530821B1 (en) * 2014-12-19 2023-05-10 리제너론 파마슈티칼스 인코포레이티드 Methods and compositions for targeted genetic modification through single-step multiple targeting
ES2955841T3 (en) 2015-03-16 2023-12-07 Regeneron Pharma Cell from a rodent that has decreased upper and lower motor neuron function and sensory perception
KR102377189B1 (en) 2015-05-29 2022-03-22 리제너론 파마슈티칼스 인코포레이티드 C9orf72 - non-human animals having a disruption in a c9orf72 locus
AU2017336100B2 (en) 2016-09-30 2023-11-30 Regeneron Pharmaceuticals, Inc. Non-human animals having a hexanucleotide repeat expansion in a C9ORF72 locus
US11696572B2 (en) 2017-06-27 2023-07-11 Regeneron Pharmaceuticals, Inc. Non-human animals comprising a humanized ASGR1 locus
CA3067872A1 (en) 2017-07-31 2019-02-07 Regeneron Pharmaceuticals, Inc. Cas-transgenic mouse embryonic stem cells and mice and uses thereof
CN110891419A (en) 2017-07-31 2020-03-17 瑞泽恩制药公司 Evaluation of CRISPR/CAS-induced in vivo recombination with exogenous donor nucleic acids
CA3068072A1 (en) 2017-07-31 2019-02-07 Regeneron Pharmaceuticals, Inc. Methods and compositions for assessing crispr/cas-mediated disruption or excision and crispr/cas-induced recombination with an exogenous donor nucleic acid in vivo
MX2020003589A (en) 2017-09-29 2020-07-22 Regeneron Pharma Non-human animals comprising a humanized.
EP3585163B1 (en) 2017-11-10 2022-03-09 Regeneron Pharmaceuticals, Inc. Non-human animals comprising slc30a8 mutation and methods of use
AU2018375796A1 (en) 2017-11-30 2020-04-23 Regeneron Pharmaceuticals, Inc. Non-human animals comprising a humanized TRKB locus
KR20240038811A (en) 2018-03-19 2024-03-25 리제너론 파마슈티칼스 인코포레이티드 Transcription modulation in animals using crispr/cas systems
KR102545031B1 (en) 2018-09-13 2023-06-20 리제너론 파마슈티칼스 인코포레이티드 Complement factor H gene knockout rats as a C3 glomerulopathy model
JP7449291B2 (en) 2018-12-20 2024-03-13 リジェネロン・ファーマシューティカルズ・インコーポレイテッド Nuclease-mediated repeat expansion
US11737435B2 (en) 2019-04-04 2023-08-29 Regeneron Pharmaceuticals, Inc. Non-human animals comprising a humanized coagulation factor 12 locus
EP3801011A1 (en) 2019-06-04 2021-04-14 Regeneron Pharmaceuticals, Inc. Non-human animals comprising a humanized ttr locus with a beta-slip mutation and methods of use
MX2021015122A (en) 2019-06-07 2022-04-06 Regeneron Pharma Non-human animals comprising a humanized albumin locus.
RU2722933C1 (en) * 2019-06-11 2020-06-05 Автономная некоммерческая образовательная организация высшего образования Сколковский институт науки и технологий Dna protease cutting agent based on cas9 protein from demequina sediminicola bacteria
WO2021108363A1 (en) 2019-11-25 2021-06-03 Regeneron Pharmaceuticals, Inc. Crispr/cas-mediated upregulation of humanized ttr allele
CA3169272A1 (en) 2020-01-28 2021-08-05 Regeneron Pharmaceuticals, Inc. Non-human animals comprising a humanized pnpla3 locus and methods of use
EP4099821A1 (en) 2020-02-07 2022-12-14 Regeneron Pharmaceuticals, Inc. <smallcaps/>? ? ?klkb1? ? ? ? ?non-human animals comprising a humanizedlocus and methods of use
WO2021195079A1 (en) 2020-03-23 2021-09-30 Regeneron Pharmaceuticals, Inc. Non-human animals comprising a humanized ttr locus comprising a v30m mutation and methods of use
US20230232796A1 (en) 2020-06-26 2023-07-27 Regeneron Pharmaceuticals, Inc. Non-human animals comprising a humanized ace2 locus
JP2024509194A (en) 2021-03-05 2024-02-29 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー In vivo DNA assembly and analysis
AU2022381205A1 (en) 2021-11-04 2024-03-28 Regeneron Pharmaceuticals, Inc. Non-human animals comprising a modified cacng1 locus
WO2023108047A1 (en) 2021-12-08 2023-06-15 Regeneron Pharmaceuticals, Inc. Mutant myocilin disease model and uses thereof
WO2023122506A1 (en) 2021-12-20 2023-06-29 Regeneron Pharmaceuticals, Inc. Non-human animals comprising humanized ace2 and tmprss loci
WO2023150798A1 (en) 2022-02-07 2023-08-10 Regeneron Pharmaceuticals, Inc. Compositions and methods for defining optimal treatment timeframes in lysosomal disease
US20230257432A1 (en) 2022-02-11 2023-08-17 Regeneron Pharmaceuticals, Inc. Compositions and methods for screening 4r tau targeting agents
WO2024026488A2 (en) 2022-07-29 2024-02-01 Regeneron Pharmaceuticals, Inc. Non-human animals comprising a modified transferrin receptor locus
WO2024031053A1 (en) 2022-08-05 2024-02-08 Regeneron Pharmaceuticals, Inc. Aggregation-resistant variants of tdp-43
WO2024073679A1 (en) 2022-09-29 2024-04-04 Regeneron Pharmaceuticals, Inc. Correction of hepatosteatosis in humanized liver animals through restoration of il6/il6r/gp130 signaling in human hepatocytes

Family Cites Families (95)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5830729A (en) 1996-04-18 1998-11-03 Institut Pasteur I Sce I-induced gene replacement and gene conversion in embryonic stem cells
WO1998041645A1 (en) 1997-03-14 1998-09-24 Idec Pharmaceuticals Corporation Method for integrating genes at specific sites in mammalian cells via homologous recombination and vectors for accomplishing the same
US5830698A (en) * 1997-03-14 1998-11-03 Idec Pharmaceuticals Corporation Method for integrating genes at specific sites in mammalian cells via homologous recombination and vectors for accomplishing the same
EP1141360A4 (en) 1998-12-31 2002-11-06 David Gladstone Inst Transgenic rodents and rodent cell lines expressing hiv co-receptors
JP2002535995A (en) 1999-02-03 2002-10-29 ザ チルドレンズ メディカル センター コーポレイション Gene repair involving induction of double-stranded DNA breaks at chromosomal target sites
ATE309536T1 (en) * 1999-12-06 2005-11-15 Sangamo Biosciences Inc METHODS OF USING RANDOMIZED ZINC FINGER PROTEIN LIBRARIES TO IDENTIFY GENE FUNCTIONS
US6596541B2 (en) 2000-10-31 2003-07-22 Regeneron Pharmaceuticals, Inc. Methods of modifying eukaryotic cells
US6586251B2 (en) 2000-10-31 2003-07-01 Regeneron Pharmaceuticals, Inc. Methods of modifying eukaryotic cells
US7105348B2 (en) 2000-10-31 2006-09-12 Regeneron Pharmaceuticals, Inc. Methods of modifying eukaryotic cells
US20050144655A1 (en) * 2000-10-31 2005-06-30 Economides Aris N. Methods of modifying eukaryotic cells
CN100575485C (en) 2002-01-23 2009-12-30 犹他大学研究基金会 Use the directed karyomit(e) mutagenesis of Zinc finger nuclease
CA2479858A1 (en) 2002-03-21 2003-10-02 Monika Liljedahl Methods and compositions for using zinc finger endonucleases to enhance homologous recombination
EP2806025B1 (en) 2002-09-05 2019-04-03 California Institute of Technology Use of zinc finger nucleases to stimulate gene targeting
US20030175968A1 (en) 2002-10-30 2003-09-18 Golic Kent G. Gene targeting method
JP2006517101A (en) * 2003-01-13 2006-07-20 エス. ラオ、マヘンドラ Sustained expression of candidate molecules in proliferating stem and progenitor cells to deliver therapeutic products
US8409861B2 (en) 2003-08-08 2013-04-02 Sangamo Biosciences, Inc. Targeted deletion of cellular DNA sequences
US7888121B2 (en) 2003-08-08 2011-02-15 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
EP2927318B1 (en) 2003-08-08 2020-05-20 Sangamo Therapeutics, Inc. Methods and compositions for targeted cleavage and recombination
WO2006033859A2 (en) 2004-09-16 2006-03-30 Sangamo Biosciences, Inc. Compositions and methods for protein production
DK1802193T3 (en) 2004-10-19 2014-06-10 Regeneron Pharma Method of producing a homozygous mouse for genetic modification
FR2879622B1 (en) 2004-12-17 2008-02-01 Agronomique Inst Nat Rech IN VITRO PROCESS FOR THE PRODUCTION OF OOCYTES OR EGGS WITH TARGETED GENOMIC MODIFICATION
US10022457B2 (en) 2005-08-05 2018-07-17 Gholam A. Peyman Methods to regulate polarization and enhance function of cells
GB0615327D0 (en) 2006-03-30 2006-09-13 Univ Edinburgh Culture medium containing kinase inhibitors and uses thereof
WO2007117410A2 (en) 2006-03-31 2007-10-18 Medarex, Inc. Transgenic animals expressing chimeric antibodies for use in preparing human antibodies
US7771967B2 (en) 2006-12-22 2010-08-10 The J. David Gladstone Institutes Nucleic acid encoding apolipoprotein E-I3
US10155038B2 (en) 2007-02-02 2018-12-18 Yale University Cells prepared by transient transfection and methods of use thereof
ATE489465T1 (en) 2007-04-26 2010-12-15 Sangamo Biosciences Inc TARGETED INTEGRATION INTO THE PPP1R12C POSITION
PT2602323T (en) 2007-06-01 2018-04-02 Open Monoclonal Tech Inc Compositions and methods for inhibiting endogenous immunoglobin genes and producing transgenic human idiotype antibodies
JP5908725B2 (en) 2008-08-22 2016-04-26 サンガモ バイオサイエンシーズ, インコーポレイテッド Methods and compositions for targeted single-strand cleavage and targeted integration
US20100076057A1 (en) 2008-09-23 2010-03-25 Northwestern University TARGET DNA INTERFERENCE WITH crRNA
EP2180058A1 (en) 2008-10-23 2010-04-28 Cellectis Meganuclease recombination system
EP2352369B1 (en) 2008-12-04 2017-04-26 Sangamo BioSciences, Inc. Genome editing in rats using zinc-finger nucleases
US20110239315A1 (en) 2009-01-12 2011-09-29 Ulla Bonas Modular dna-binding domains and methods of use
US8722964B2 (en) 2009-04-23 2014-05-13 Transposagen Biopharmaceuticals, Inc. Genetically engineered or transgenic rats exhibiting a cancer phenotype due to a disruption of germline tumor suppressor genes
US8772008B2 (en) 2009-05-18 2014-07-08 Sangamo Biosciences, Inc. Methods and compositions for increasing nuclease activity
US8558055B2 (en) 2009-07-24 2013-10-15 Transposagen Biopharmaceuticals, Inc. Genetically modified rat comprising a cytokine gene disruption and exhibiting a greater susceptibility to a cytokine-mediated autoimmune and/or inflammatory disease
CA2779337A1 (en) * 2009-10-28 2011-05-05 Helmholtz Zentrum Muenchen Deutsches Forschungszentrum Fuer Gesundheit U Nd Umwelt (Gmbh) Homologous recombination in the oocyte
PL2494047T3 (en) 2009-10-29 2017-05-31 Regeneron Pharmaceuticals, Inc. Multifunctional alleles
WO2011053957A2 (en) 2009-11-02 2011-05-05 Gen9, Inc. Compositions and methods for the regulation of multiple genes of interest in a cell
EP2508595B1 (en) 2009-12-01 2016-11-23 National Cancer Center Method for constructing chimeric rat using rat embryonic stem cells
PL2816112T3 (en) 2009-12-10 2019-03-29 Regents Of The University Of Minnesota Tal effector-mediated DNA modification
EP2516652B1 (en) 2009-12-21 2014-11-05 Keygene N.V. Improved techniques for transfecting protoplasts
CN102843904B (en) 2010-01-22 2015-01-21 陶氏益农公司 Excision of transgenes in genetically modified organisms
WO2011100058A1 (en) * 2010-02-09 2011-08-18 Sangamo Biosciences, Inc. Targeted genomic modification with partially single-stranded donor molecules
WO2011146121A1 (en) 2010-05-17 2011-11-24 Sangamo Biosciences, Inc. Novel dna-binding proteins and uses thereof
GB201009732D0 (en) * 2010-06-10 2010-07-21 Gene Bridges Gmbh Direct cloning
EP2579711B1 (en) 2010-06-11 2019-04-17 Regeneron Pharmaceuticals, Inc. Production of fertile xy female animals from xy es cells
US8945868B2 (en) * 2010-07-21 2015-02-03 Sangamo Biosciences, Inc. Methods and compositions for modification of a HLA locus
WO2012018726A1 (en) 2010-08-02 2012-02-09 Cellectis Sa Method for increasing double-strand break-induced gene targeting
WO2012129198A1 (en) 2011-03-23 2012-09-27 Transposagen Biopharmaceuticals, Inc. Genetically modified rat models for obesity and diabetes
WO2012168307A2 (en) 2011-06-07 2012-12-13 Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) Improved recombination efficiency by inhibition of nhej dna repair
IL305550A (en) 2011-10-28 2023-10-01 Regeneron Pharma Genetically modified t cell receptor mice
WO2013141680A1 (en) 2012-03-20 2013-09-26 Vilnius University RNA-DIRECTED DNA CLEAVAGE BY THE Cas9-crRNA COMPLEX
US9637739B2 (en) 2012-03-20 2017-05-02 Vilnius University RNA-directed DNA cleavage by the Cas9-crRNA complex
SG10201702445TA (en) 2012-04-25 2017-04-27 Regeneron Pharma Nuclease-mediated targeting with large targeting vectors
CA2871524C (en) 2012-05-07 2021-07-27 Sangamo Biosciences, Inc. Methods and compositions for nuclease-mediated targeted integration of transgenes
RS64622B1 (en) 2012-05-25 2023-10-31 Univ California Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription
MX2014015204A (en) 2012-06-12 2015-08-07 Genentech Inc Methods and compositions for generating conditional knock-out alleles.
ES2757623T3 (en) 2012-07-25 2020-04-29 Broad Inst Inc Inducible DNA binding proteins and genomic disruption tools and applications thereof
JP6517143B2 (en) 2012-10-23 2019-05-22 ツールゲン インコーポレイテッド Composition for cleaving target DNA comprising guide RNA specific for target DNA and CAS protein encoding nucleic acid or CAS protein, and use thereof
KR102006880B1 (en) 2012-12-06 2019-08-02 시그마-알드리치 컴퍼니., 엘엘씨 Crispr-based genome modification and regulation
WO2014093479A1 (en) 2012-12-11 2014-06-19 Montana State University Crispr (clustered regularly interspaced short palindromic repeats) rna-guided control of gene regulation
WO2014093701A1 (en) 2012-12-12 2014-06-19 The Broad Institute, Inc. Functional genomics using crispr-cas systems, compositions, methods, knock out libraries and applications thereof
PT2896697E (en) 2012-12-12 2015-12-31 Massachusetts Inst Technology Engineering of systems, methods and optimized guide compositions for sequence manipulation
DK2931898T3 (en) 2012-12-12 2016-06-20 Massachusetts Inst Technology CONSTRUCTION AND OPTIMIZATION OF SYSTEMS, PROCEDURES AND COMPOSITIONS FOR SEQUENCE MANIPULATION WITH FUNCTIONAL DOMAINS
WO2014093694A1 (en) 2012-12-12 2014-06-19 The Broad Institute, Inc. Crispr-cas nickase systems, methods and compositions for sequence manipulation in eukaryotes
US8697359B1 (en) 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
WO2014093718A1 (en) 2012-12-12 2014-06-19 The Broad Institute, Inc. Methods, systems, and apparatus for identifying target sequences for cas enzymes or crispr-cas systems for target sequences and conveying results thereof
IL239317B (en) 2012-12-12 2022-07-01 Broad Inst Inc Delivery, engineering and optimization of systems, methods and compositions for sequence manipulation and therapeutic applications
PT2898075E (en) 2012-12-12 2016-06-16 Harvard College Engineering and optimization of improved systems, methods and enzyme compositions for sequence manipulation
EP4286402A3 (en) 2012-12-12 2024-02-14 The Broad Institute, Inc. Crispr-cas component systems, methods and compositions for sequence manipulation
EP3434776A1 (en) 2012-12-12 2019-01-30 The Broad Institute, Inc. Methods, models, systems, and apparatus for identifying target sequences for cas enzymes or crispr-cas systems for target sequences and conveying results thereof
SG11201504621RA (en) 2012-12-17 2015-07-30 Harvard College Rna-guided human genome engineering
FI3491915T3 (en) 2012-12-27 2023-08-29 Keygene Nv Method for inducing a targeted translocation in a plant.
WO2014127287A1 (en) 2013-02-14 2014-08-21 Massachusetts Institute Of Technology Method for in vivo tergated mutagenesis
ES2904803T3 (en) 2013-02-20 2022-04-06 Regeneron Pharma Genetic modification of rats
AU2014218621B2 (en) 2013-02-25 2019-11-07 Sangamo Therapeutics, Inc. Methods and compositions for enhancing nuclease-mediated gene disruption
WO2014131833A1 (en) 2013-02-27 2014-09-04 Helmholtz Zentrum München Deutsches Forschungszentrum Für Gesundheit Und Umwelt (Gmbh) Gene editing in the oocyte by cas9 nucleases
US10612043B2 (en) 2013-03-09 2020-04-07 Agilent Technologies, Inc. Methods of in vivo engineering of large sequences using multiple CRISPR/cas selections of recombineering events
ES2901396T3 (en) 2013-03-14 2022-03-22 Caribou Biosciences Inc Nucleic Acid Targeting Nucleic Acid Compositions and Methods
US9234213B2 (en) 2013-03-15 2016-01-12 System Biosciences, Llc Compositions and methods directed to CRISPR/Cas genomic engineering systems
US20140349400A1 (en) 2013-03-15 2014-11-27 Massachusetts Institute Of Technology Programmable Modification of DNA
US20140273230A1 (en) 2013-03-15 2014-09-18 Sigma-Aldrich Co., Llc Crispr-based genome modification and regulation
EP2975942B1 (en) 2013-03-21 2018-08-08 Sangamo Therapeutics, Inc. Targeted disruption of t cell receptor genes using engineered zinc finger protein nucleases
JP2016522679A (en) 2013-04-04 2016-08-04 プレジデント アンド フェローズ オブ ハーバード カレッジ Therapeutic use of genome editing with the CRISPR / Cas system
RS58255B1 (en) * 2013-04-16 2019-03-29 Regeneron Pharma Targeted modification of rat genome
US20160040155A1 (en) 2013-04-16 2016-02-11 University Of Washington Through Its Center For Commercialization Activating an alternative pathway for homology-directed repair to stimulate targeted gene correction and genome engineering
WO2014172470A2 (en) 2013-04-16 2014-10-23 Whitehead Institute For Biomedical Research Methods of mutating, modifying or modulating nucleic acid in a cell or nonhuman mammal
EP2796558A1 (en) 2013-04-23 2014-10-29 Rheinische Friedrich-Wilhelms-Universität Bonn Improved gene targeting and nucleic acid carrier molecule, in particular for use in plants
US11306328B2 (en) 2013-07-26 2022-04-19 President And Fellows Of Harvard College Genome engineering
WO2015088643A1 (en) 2013-12-11 2015-06-18 Regeneron Pharmaceuticals, Inc. Methods and compositions for the targeted modification of a genome
RU2704283C9 (en) 2014-06-06 2020-02-07 Регенерон Фармасьютикалз, Инк. Methods and compositions for modifying target locus
ES2694629T3 (en) 2014-06-26 2018-12-26 Regeneron Pharmaceuticals, Inc. Methods and compositions for objective genetic modifications and methods of use
CA2954414A1 (en) 2014-07-15 2016-01-21 Juno Therapeutics, Inc. Engineered cells for adoptive cell therapy
KR102530821B1 (en) 2014-12-19 2023-05-10 리제너론 파마슈티칼스 인코포레이티드 Methods and compositions for targeted genetic modification through single-step multiple targeting

Also Published As

Publication number Publication date
CY1120572T1 (en) 2019-07-10
US20130309670A1 (en) 2013-11-21
KR20150004816A (en) 2015-01-13
US10301646B2 (en) 2019-05-28
AU2013251558B2 (en) 2019-01-03
ES2683071T3 (en) 2018-09-24
CA2869016A1 (en) 2013-10-31
SG10201702445TA (en) 2017-04-27
MX362523B (en) 2019-01-22
CN104364380B (en) 2018-10-09
PL2847335T3 (en) 2019-01-31
HK1207396A1 (en) 2016-01-29
PT2847335T (en) 2018-09-28
EP2847335A1 (en) 2015-03-18
AU2013251558A1 (en) 2014-10-02
WO2013163394A1 (en) 2013-10-31
EP2847335B1 (en) 2018-06-27
US20180037910A1 (en) 2018-02-08
IL234905B (en) 2018-10-31
DK2847335T3 (en) 2018-08-13
CN104364380A (en) 2015-02-18
CN109536526A (en) 2019-03-29
US9834786B2 (en) 2017-12-05
JP6275120B2 (en) 2018-02-07
MX2014012994A (en) 2015-02-04
CA2869016C (en) 2020-05-05
KR101904508B1 (en) 2018-10-04
CN109536526B (en) 2020-06-09
IN2014DN09261A (en) 2015-07-10
JP2015514439A (en) 2015-05-21
BR112014026294A2 (en) 2020-07-07
RU2014145942A (en) 2016-06-10
RU2645475C2 (en) 2018-02-21

Similar Documents

Publication Publication Date Title
HK1207396A1 (en) Nuclease-mediated targeting with large targeting vectors
IL236563A (en) Search-powered connection targeting
GB201217807D0 (en) Location-assisted beamforming
EP2940755A4 (en) Assembled battery
EP2866324A4 (en) Charger
AU347859S (en) Battery
AU346864S (en) Battery
PL2713471T3 (en) Charger
HK1180931A1 (en) An electro-pulsograph
ZA201500911B (en) Enzymes
EP2887796A4 (en) Multiple-virus-resistant melon
EP2874517A4 (en) Seat-lift assembly
GB2506566B (en) Pole piece
GB201212340D0 (en) Antennas
GB201420527D0 (en) Electrical-wire-protecting member
EP2916451A4 (en) Electricity-generating system
EP2812436A4 (en) Mini-intronic plasmid vectors
EP2865582A4 (en) Frp member
EP2899772A4 (en) Battery assembly
GB201214720D0 (en) Maser assembly
EP2874204A4 (en) Battery assembly
EP2905847A4 (en) Assembly
EP2824757A4 (en) Assembled battery
GB201206455D0 (en) Gene expression
GB2518076B (en) Electrical-wire-protecting member