JP4183742B1 - 誘導多能性幹細胞の製造方法 - Google Patents
誘導多能性幹細胞の製造方法 Download PDFInfo
- Publication number
- JP4183742B1 JP4183742B1 JP2008131577A JP2008131577A JP4183742B1 JP 4183742 B1 JP4183742 B1 JP 4183742B1 JP 2008131577 A JP2008131577 A JP 2008131577A JP 2008131577 A JP2008131577 A JP 2008131577A JP 4183742 B1 JP4183742 B1 JP 4183742B1
- Authority
- JP
- Japan
- Prior art keywords
- cells
- gene
- cell
- genes
- ips
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 210000004263 induced pluripotent stem cell Anatomy 0.000 title claims abstract description 36
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 5
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 233
- 210000001082 somatic cell Anatomy 0.000 claims abstract description 56
- 101150086694 SLC22A3 gene Proteins 0.000 claims abstract description 30
- 101710135898 Myc proto-oncogene protein Proteins 0.000 claims abstract description 26
- 101710150448 Transcriptional regulator Myc Proteins 0.000 claims abstract description 26
- 102100038895 Myc proto-oncogene protein Human genes 0.000 claims abstract description 25
- 101100247004 Rattus norvegicus Qsox1 gene Proteins 0.000 claims abstract description 25
- 108700021430 Kruppel-Like Factor 4 Proteins 0.000 claims abstract description 20
- 210000004027 cell Anatomy 0.000 abstract description 202
- 238000000034 method Methods 0.000 abstract description 39
- 230000035755 proliferation Effects 0.000 abstract description 14
- 210000002257 embryonic structure Anatomy 0.000 abstract description 13
- 230000001939 inductive effect Effects 0.000 abstract description 4
- 210000001671 embryonic stem cell Anatomy 0.000 description 83
- 230000008672 reprogramming Effects 0.000 description 77
- 241000699666 Mus <mouse, genus> Species 0.000 description 35
- 230000014509 gene expression Effects 0.000 description 27
- 241000699670 Mus sp. Species 0.000 description 20
- 239000005090 green fluorescent protein Substances 0.000 description 18
- 108010043121 Green Fluorescent Proteins Proteins 0.000 description 16
- 102000004144 Green Fluorescent Proteins Human genes 0.000 description 16
- 210000002950 fibroblast Anatomy 0.000 description 16
- 241001430294 unidentified retrovirus Species 0.000 description 15
- 102000003974 Fibroblast growth factor 2 Human genes 0.000 description 12
- 108090000379 Fibroblast growth factor 2 Proteins 0.000 description 12
- 230000004069 differentiation Effects 0.000 description 11
- 108091057508 Myc family Proteins 0.000 description 9
- 229940079593 drug Drugs 0.000 description 9
- 239000003814 drug Substances 0.000 description 9
- 102000004127 Cytokines Human genes 0.000 description 8
- 108090000695 Cytokines Proteins 0.000 description 8
- 238000011161 development Methods 0.000 description 8
- 230000018109 developmental process Effects 0.000 description 8
- 239000003550 marker Substances 0.000 description 8
- 210000004940 nucleus Anatomy 0.000 description 8
- 102000004169 proteins and genes Human genes 0.000 description 8
- RXWNCPJZOCPEPQ-NVWDDTSBSA-N puromycin Chemical compound C1=CC(OC)=CC=C1C[C@H](N)C(=O)N[C@H]1[C@@H](O)[C@H](N2C3=NC=NC(=C3N=C2)N(C)C)O[C@@H]1CO RXWNCPJZOCPEPQ-NVWDDTSBSA-N 0.000 description 8
- 210000001626 skin fibroblast Anatomy 0.000 description 8
- 108020004414 DNA Proteins 0.000 description 7
- 241000699660 Mus musculus Species 0.000 description 7
- 201000010099 disease Diseases 0.000 description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 7
- 230000004720 fertilization Effects 0.000 description 7
- 230000006698 induction Effects 0.000 description 7
- 238000003757 reverse transcription PCR Methods 0.000 description 7
- 238000012216 screening Methods 0.000 description 7
- 101150072531 10 gene Proteins 0.000 description 6
- -1 Dnmt3L Proteins 0.000 description 6
- 101100011486 Mus musculus Elf4 gene Proteins 0.000 description 6
- 206010028980 Neoplasm Diseases 0.000 description 6
- 210000002459 blastocyst Anatomy 0.000 description 6
- 210000003754 fetus Anatomy 0.000 description 6
- 230000004927 fusion Effects 0.000 description 6
- 238000011580 nude mouse model Methods 0.000 description 6
- 239000013598 vector Substances 0.000 description 6
- 102000015735 Beta-catenin Human genes 0.000 description 5
- 108060000903 Beta-catenin Proteins 0.000 description 5
- 241000282412 Homo Species 0.000 description 5
- 101100540311 Human papillomavirus type 16 E6 gene Proteins 0.000 description 5
- 101000767631 Human papillomavirus type 16 Protein E7 Proteins 0.000 description 5
- 101710128836 Large T antigen Proteins 0.000 description 5
- 101150001847 Sox15 gene Proteins 0.000 description 5
- 239000002771 cell marker Substances 0.000 description 5
- 230000008859 change Effects 0.000 description 5
- 210000002242 embryoid body Anatomy 0.000 description 5
- 230000001605 fetal effect Effects 0.000 description 5
- 230000006870 function Effects 0.000 description 5
- 210000003494 hepatocyte Anatomy 0.000 description 5
- 210000000130 stem cell Anatomy 0.000 description 5
- 238000002054 transplantation Methods 0.000 description 5
- 102100037124 Developmental pluripotency-associated 5 protein Human genes 0.000 description 4
- 241000588724 Escherichia coli Species 0.000 description 4
- 101000881848 Homo sapiens Developmental pluripotency-associated 5 protein Proteins 0.000 description 4
- 101000804949 Mus musculus Developmental pluripotency-associated protein 2 Proteins 0.000 description 4
- 101100390493 Mus musculus Fbxo15 gene Proteins 0.000 description 4
- 101150099493 STAT3 gene Proteins 0.000 description 4
- 108091023040 Transcription factor Proteins 0.000 description 4
- 102000040945 Transcription factor Human genes 0.000 description 4
- 230000024245 cell differentiation Effects 0.000 description 4
- 230000004663 cell proliferation Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 210000004602 germ cell Anatomy 0.000 description 4
- 210000002149 gonad Anatomy 0.000 description 4
- 210000002569 neuron Anatomy 0.000 description 4
- 229950010131 puromycin Drugs 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 4
- 101150094083 24 gene Proteins 0.000 description 3
- 101150033839 4 gene Proteins 0.000 description 3
- 101100015729 Drosophila melanogaster drk gene Proteins 0.000 description 3
- 101000971801 Homo sapiens KH domain-containing protein 3 Proteins 0.000 description 3
- 101000889749 Homo sapiens Putative ATP-dependent RNA helicase TDRD12 Proteins 0.000 description 3
- 101000777245 Homo sapiens Undifferentiated embryonic cell transcription factor 1 Proteins 0.000 description 3
- 102100021450 KH domain-containing protein 3 Human genes 0.000 description 3
- 241000713666 Lentivirus Species 0.000 description 3
- 241000124008 Mammalia Species 0.000 description 3
- 101100058550 Mus musculus Bmi1 gene Proteins 0.000 description 3
- 101000881849 Mus musculus Developmental pluripotency-associated protein 4 Proteins 0.000 description 3
- 101710150336 Protein Rex Proteins 0.000 description 3
- 102100040195 Putative ATP-dependent RNA helicase TDRD12 Human genes 0.000 description 3
- 101150047500 TERT gene Proteins 0.000 description 3
- 206010043276 Teratoma Diseases 0.000 description 3
- 102100031278 Undifferentiated embryonic cell transcription factor 1 Human genes 0.000 description 3
- 101000929049 Xenopus tropicalis Derriere protein Proteins 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 210000004413 cardiac myocyte Anatomy 0.000 description 3
- 238000004113 cell culture Methods 0.000 description 3
- 210000003855 cell nucleus Anatomy 0.000 description 3
- 230000002500 effect on skin Effects 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 108020001507 fusion proteins Proteins 0.000 description 3
- 102000037865 fusion proteins Human genes 0.000 description 3
- 101150098203 grb2 gene Proteins 0.000 description 3
- 210000004185 liver Anatomy 0.000 description 3
- 210000001161 mammalian embryo Anatomy 0.000 description 3
- 239000013612 plasmid Substances 0.000 description 3
- 108090000765 processed proteins & peptides Proteins 0.000 description 3
- 230000001105 regulatory effect Effects 0.000 description 3
- 238000010186 staining Methods 0.000 description 3
- 238000010361 transduction Methods 0.000 description 3
- 230000026683 transduction Effects 0.000 description 3
- 238000011740 C57BL/6 mouse Methods 0.000 description 2
- 101100257372 Caenorhabditis elegans sox-3 gene Proteins 0.000 description 2
- 230000007067 DNA methylation Effects 0.000 description 2
- 206010059866 Drug resistance Diseases 0.000 description 2
- YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 description 2
- 102100037362 Fibronectin Human genes 0.000 description 2
- 108010067306 Fibronectins Proteins 0.000 description 2
- 102100039289 Glial fibrillary acidic protein Human genes 0.000 description 2
- 101710193519 Glial fibrillary acidic protein Proteins 0.000 description 2
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Chemical compound C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 description 2
- 101000881866 Homo sapiens Developmental pluripotency-associated protein 3 Proteins 0.000 description 2
- WOBHKFSMXKNTIM-UHFFFAOYSA-N Hydroxyethyl methacrylate Chemical compound CC(=C)C(=O)OCCO WOBHKFSMXKNTIM-UHFFFAOYSA-N 0.000 description 2
- 101150002416 Igf2 gene Proteins 0.000 description 2
- 101150072501 Klf2 gene Proteins 0.000 description 2
- 108091054455 MAP kinase family Proteins 0.000 description 2
- 102000043136 MAP kinase family Human genes 0.000 description 2
- 229930192392 Mitomycin Natural products 0.000 description 2
- 101100310657 Mus musculus Sox1 gene Proteins 0.000 description 2
- 101100310648 Mus musculus Sox17 gene Proteins 0.000 description 2
- 101100257376 Mus musculus Sox3 gene Proteins 0.000 description 2
- NWIBSHFKIJFRCO-WUDYKRTCSA-N Mytomycin Chemical compound C1N2C(C(C(C)=C(N)C3=O)=O)=C3[C@@H](COC(N)=O)[C@@]2(OC)[C@@H]2[C@H]1N2 NWIBSHFKIJFRCO-WUDYKRTCSA-N 0.000 description 2
- 108700026495 N-Myc Proto-Oncogene Proteins 0.000 description 2
- 102100030124 N-myc proto-oncogene protein Human genes 0.000 description 2
- 101150012532 NANOG gene Proteins 0.000 description 2
- 229930193140 Neomycin Natural products 0.000 description 2
- 208000018737 Parkinson disease Diseases 0.000 description 2
- 101150024531 Slc7a1 gene Proteins 0.000 description 2
- 241000862969 Stella Species 0.000 description 2
- 102000004243 Tubulin Human genes 0.000 description 2
- 108090000704 Tubulin Proteins 0.000 description 2
- 206010067584 Type 1 diabetes mellitus Diseases 0.000 description 2
- 230000002159 abnormal effect Effects 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 210000004507 artificial chromosome Anatomy 0.000 description 2
- 210000001130 astrocyte Anatomy 0.000 description 2
- 102000005936 beta-Galactosidase Human genes 0.000 description 2
- 108010005774 beta-Galactosidase Proteins 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 210000000170 cell membrane Anatomy 0.000 description 2
- 230000005757 colony formation Effects 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 235000013601 eggs Nutrition 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000003623 enhancer Substances 0.000 description 2
- 239000013604 expression vector Substances 0.000 description 2
- 238000010353 genetic engineering Methods 0.000 description 2
- 210000001654 germ layer Anatomy 0.000 description 2
- 210000005046 glial fibrillary acidic protein Anatomy 0.000 description 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
- 229960004857 mitomycin Drugs 0.000 description 2
- 108700024542 myc Genes Proteins 0.000 description 2
- 229960004927 neomycin Drugs 0.000 description 2
- 210000004248 oligodendroglia Anatomy 0.000 description 2
- 230000001766 physiological effect Effects 0.000 description 2
- 230000002062 proliferating effect Effects 0.000 description 2
- 108020003175 receptors Proteins 0.000 description 2
- 102000005962 receptors Human genes 0.000 description 2
- 230000001177 retroviral effect Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 231100000167 toxic agent Toxicity 0.000 description 2
- 239000003440 toxic substance Substances 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 230000002103 transcriptional effect Effects 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 238000011830 transgenic mouse model Methods 0.000 description 2
- 210000004291 uterus Anatomy 0.000 description 2
- 108700026220 vif Genes Proteins 0.000 description 2
- 230000009385 viral infection Effects 0.000 description 2
- 238000011714 129 mouse Methods 0.000 description 1
- 101150028074 2 gene Proteins 0.000 description 1
- 101150029857 23 gene Proteins 0.000 description 1
- 101150090724 3 gene Proteins 0.000 description 1
- 101150106774 9 gene Proteins 0.000 description 1
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 101100342337 Caenorhabditis elegans klf-1 gene Proteins 0.000 description 1
- 102000016362 Catenins Human genes 0.000 description 1
- 108010067316 Catenins Proteins 0.000 description 1
- 241000282693 Cercopithecidae Species 0.000 description 1
- 102100026735 Coagulation factor VIII Human genes 0.000 description 1
- 108091026890 Coding region Proteins 0.000 description 1
- 108020004635 Complementary DNA Proteins 0.000 description 1
- 238000000018 DNA microarray Methods 0.000 description 1
- 230000004543 DNA replication Effects 0.000 description 1
- 241000283073 Equus caballus Species 0.000 description 1
- 108700039887 Essential Genes Proteins 0.000 description 1
- 102100038652 Ferritin heavy polypeptide-like 17 Human genes 0.000 description 1
- 101710138094 Ferritin heavy polypeptide-like 17 Proteins 0.000 description 1
- 101150066002 GFP gene Proteins 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 208000021309 Germ cell tumor Diseases 0.000 description 1
- 102000009465 Growth Factor Receptors Human genes 0.000 description 1
- 108010009202 Growth Factor Receptors Proteins 0.000 description 1
- 102100035364 Growth/differentiation factor 3 Human genes 0.000 description 1
- 206010019280 Heart failures Diseases 0.000 description 1
- 101000911390 Homo sapiens Coagulation factor VIII Proteins 0.000 description 1
- 101001023986 Homo sapiens Growth/differentiation factor 3 Proteins 0.000 description 1
- 101001139134 Homo sapiens Krueppel-like factor 4 Proteins 0.000 description 1
- 241000725303 Human immunodeficiency virus Species 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- 102100020677 Krueppel-like factor 4 Human genes 0.000 description 1
- 101100355655 Mus musculus Eras gene Proteins 0.000 description 1
- 101100446513 Mus musculus Fgf4 gene Proteins 0.000 description 1
- 101100404103 Mus musculus Nanog gene Proteins 0.000 description 1
- 101100137157 Mus musculus Pou5f1 gene Proteins 0.000 description 1
- 101100094847 Mus musculus Slc22a3 gene Proteins 0.000 description 1
- 101100310650 Mus musculus Sox18 gene Proteins 0.000 description 1
- 101100043062 Mus musculus Sox7 gene Proteins 0.000 description 1
- 208000034176 Neoplasms, Germ Cell and Embryonal Diseases 0.000 description 1
- 102000008730 Nestin Human genes 0.000 description 1
- 108010088225 Nestin Proteins 0.000 description 1
- 238000010222 PCR analysis Methods 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- 208000005074 Retroviridae Infections Diseases 0.000 description 1
- 102000037054 SLC-Transporter Human genes 0.000 description 1
- 108091006207 SLC-Transporter Proteins 0.000 description 1
- 108700019146 Transgenes Proteins 0.000 description 1
- 102000001742 Tumor Suppressor Proteins Human genes 0.000 description 1
- 108010040002 Tumor Suppressor Proteins Proteins 0.000 description 1
- 102000018265 Virus Receptors Human genes 0.000 description 1
- 108010066342 Virus Receptors Proteins 0.000 description 1
- 230000004156 Wnt signaling pathway Effects 0.000 description 1
- SHGAZHPCJJPHSC-YCNIQYBTSA-N all-trans-retinoic acid Chemical compound OC(=O)\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C SHGAZHPCJJPHSC-YCNIQYBTSA-N 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 238000001369 bisulfite sequencing Methods 0.000 description 1
- 210000004703 blastocyst inner cell mass Anatomy 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 210000000845 cartilage Anatomy 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 230000032459 dedifferentiation Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 230000013020 embryo development Effects 0.000 description 1
- YQGOJNYOYNNSMM-UHFFFAOYSA-N eosin Chemical compound [Na+].OC(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C(O)=C(Br)C=C21 YQGOJNYOYNNSMM-UHFFFAOYSA-N 0.000 description 1
- 238000000684 flow cytometry Methods 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 210000001156 gastric mucosa Anatomy 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 239000003862 glucocorticoid Substances 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 210000003128 head Anatomy 0.000 description 1
- 210000002216 heart Anatomy 0.000 description 1
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 208000000509 infertility Diseases 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 231100000535 infertility Toxicity 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 229940125396 insulin Drugs 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 208000032839 leukemia Diseases 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 230000015654 memory Effects 0.000 description 1
- 238000010172 mouse model Methods 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 210000005055 nestin Anatomy 0.000 description 1
- 210000003061 neural cell Anatomy 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 108010055896 polyornithine Proteins 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 229930002330 retinoic acid Natural products 0.000 description 1
- 238000010839 reverse transcription Methods 0.000 description 1
- 102200044943 rs121913400 Human genes 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 210000000278 spinal cord Anatomy 0.000 description 1
- 208000020431 spinal cord injury Diseases 0.000 description 1
- 230000010473 stable expression Effects 0.000 description 1
- 238000009168 stem cell therapy Methods 0.000 description 1
- 238000011476 stem cell transplantation Methods 0.000 description 1
- 238000009580 stem-cell therapy Methods 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 229960001727 tretinoin Drugs 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4702—Regulators; Modulating activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/14—Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
- A61P25/16—Anti-Parkinson drugs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
- A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/04—Inotropic agents, i.e. stimulants of cardiac contraction; Drugs for heart failure
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/602—Sox-2
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/603—Oct-3/4
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/604—Klf-4
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/605—Nanog
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/606—Transcription factors c-Myc
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2799/00—Uses of viruses
- C12N2799/02—Uses of viruses as vector
- C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
- C12N2799/027—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a retrovirus
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Neurosurgery (AREA)
- Cardiology (AREA)
- Cell Biology (AREA)
- Toxicology (AREA)
- Neurology (AREA)
- Gastroenterology & Hepatology (AREA)
- Diabetes (AREA)
- Transplantation (AREA)
- Developmental Biology & Embryology (AREA)
- Heart & Thoracic Surgery (AREA)
- Hospice & Palliative Care (AREA)
- Hematology (AREA)
- Endocrinology (AREA)
- Urology & Nephrology (AREA)
Abstract
【解決手段】体細胞から誘導多能性幹細胞を製造する方法であって、下記の4種の遺伝子:Oct3/4、Klf4、c-Myc、及びSox2を体細胞に導入する工程を含む方法。
【選択図】なし
Description
また、別の好ましい態様によれば、下記の遺伝子:Soxファミリー遺伝子の遺伝子産物をさらに含む上記の因子も提供され、より好ましい態様としてSox2の遺伝子産物を含む上記の因子が提供される。
さらに別の好ましい態様によれば、Mycファミリー遺伝子の遺伝子産物とともに、あるいはMycファミリー遺伝子の遺伝子産物に換えてサイトカインを含む上記の因子が提供され、より好ましい態様としてサイトカインがbasic fibroblast growth factor (bFGF)及び/又はStem Cell Factor (SCF) である上記の因子が提供される。
これらの因子に加えて、下記の群:Fbx15、Nanog、ERas、ECAT15-2、Tcl1、及びβ-cateninからなる群から選ばれる1種以上の遺伝子の遺伝子産物をさらに含む上記の因子が提供される。
また、上記発明の別の好ましい態様によれば、下記の群:ECAT1、Esg1、Dnmt3L、ECAT8、Gdf3、Sox15、ECAT15-1、Fthl17、Sall4、Rex1、UTF1、Stella、Stat3、及びGrb2からなる群から選ばれる1種以上の遺伝子の遺伝子産物をさらに含む上記の因子も提供される。
この発明の好ましい態様によれば、体細胞の培養物中に上記の核初期化因子を添加する工程を含む上記の方法;体細胞に上記の核初期化因子をコードする遺伝子を導入する工程を含む上記の方法;上記の核初期化因子をコードする遺伝子を少なくとも1種以上含む組換えベクターを用いて体細胞に該遺伝子を導入する工程を含む上記の方法;及び体細胞として患者から採取した体細胞を用いる上記の方法が提供される。
さらに本発明により、幹細胞療法であって、患者から分離採取した体細胞を用いて上記の方法により得られた誘導多能性幹細胞を分化誘導して得られる体細胞を該患者に移植する工程を含む療法が提供される。
さらに本発明により、上記の方法により得られた誘導多能性幹細胞を分化誘導して得られる各種細胞を用いて、化合物、薬剤、毒物などの生理作用や毒性を評価する方法が提供される。
また、本発明により、細胞の分化能及び/又は増殖能を改善する方法であって、細胞に対して上記の核初期化因子を接触させる工程を含む方法、並びに上記方法により得られた細胞及び上記方法により得られた細胞から分化誘導された体細胞が提供される。
本発明の核初期化因子を確認する手段としては、例えば、国際公開WO 2005/80598に記載された核初期化因子のスクリーニング方法を利用することができる。上記刊行物の全ての開示を参照により本明細書の開示に含める。当業者は上記刊行物を参照することにより核初期化因子をスクリーニングし、本発明の初期化因子の存在及び作用を確認することができる。
さらに好ましい態様として、上記の3種類の遺伝子産物、好ましくは上記の4種類の遺伝子産物に加えて、細胞の不死化を誘導する因子をあげることができる。たとえば、TERT遺伝子の遺伝子産物を含む因子と、下記の遺伝子:SV40 Large T antigen、HPV16 E6、HPV16 E7、及びBmilからなる群から選ばれる1種以上の遺伝子の遺伝子産物を含む因子を、組み合わせることを挙げることができる。TERTはDNA複製時における染色体末端テロメア構造維持のために必須であり、ヒトでは幹細胞や腫瘍細胞では発現するが、多くの体細胞においては発現が認められない(I. Horikawa, et al., Proc Natl Acad Sci USA. 102, pp18437-442, 2005)。SV40 Large T antigen、HPV16 E6、HPV16 E7、またはBmilは、Large T antigenと組み合わせることにより、ヒト体細胞の不死化をもたらすことが報告されている(S. Akimov et al., Stem Cells, 23, pp1423-1433, 2005; P. Salmon et al., Mol. Ther.,2, pp404-414, 2000)。これらの因子は、特にヒト細胞からiPS細胞を誘導する場合において極めて有用である。TERTおよびBmi1遺伝子のNCBIアセッション番号は以下のとおりである。
例1:初期化因子の選択
初期化因子を同定するためには初期化現象を容易に観察する実験系が必要である。実験系としてFbx15遺伝子座にβgeo(ベータガラクトシダーゼとネオマイシン耐性遺伝子の融合遺伝子)をノックインしたマウスを利用した。マウスFbx15遺伝子はES細胞や初期胚といった分化多能性細胞において特異的に発現する遺伝子である。しかしマウスFbx15遺伝子にβgeoをノックインし、Fbx15の機能を欠失したホモ変異マウスにおいては、分化多能性や発生を含めて異常な表現型は観察されなかった。このマウスにおいては、βgeoがFbx15遺伝子のエンハンサーやプロモーターにより発現制御される。すなわち、分化した体細胞ではβgeoは発現せず、G418に感受性を示す。一方、βgeoをノックインしたホモ変異ES細胞は極めて高濃度(12 mg/ml以上)のG418に耐性を示す。この現象を利用し、体細胞の初期化を可視化する実験系を構築した。
10種類の遺伝子群のなかで特に重要性が示唆された4遺伝子により体細胞の初期化の誘導が可能であるか否かを検討した。Fbx15遺伝子にβgeoをノックインしたMEF細胞に上記10種類の遺伝子の組み合わせ、上記4種類の遺伝子の組み合わせ、上記4種類のうち3種類のみの遺伝子の組み合わせ、及び上記4種類のうち2種類のみの遺伝子の組み合わせを用いて、これらの遺伝子群をレトロウイルスにより体細胞に導入した。その結果、4種類の遺伝子を導入した場合には160個のG418耐性コロニーが得られた。この結果は、10種類の遺伝子を導入した場合の結果(179コロニー)とほぼ同数であったが、4遺伝子導入の場合には10遺伝子導入の場合に比べてコロニーが小さかった。また、これらのコロニーを継代培養した場合、iPS細胞の形態を示したコロニーは10遺伝子導入の場合に12クローン中9クローンであったのに対して、4遺伝子導入の場合には12クローン中7クローンと若干少ない傾向にあった。4遺伝子としては、マウス由来のもの、ヒト由来のもの、どちらでもほぼ同じ数のiPS細胞が得られた。
これらの結果から、初期化のためには少なくとも3遺伝子の組合せ(#14、#20、及び#22)が必須であり、それらの3種の遺伝子を含む4遺伝子群及び10遺伝子群では遺伝子の数を増やすにつれて初期化の効率が上昇することが明らかとなった。
樹立したiPS細胞の分化多能性を評価するため、24因子、10因子、および4因子で樹立されたiPS細胞をヌードマウスの皮下に移植した。その結果、ES細胞と同様の大きさの腫瘍が全例で形成された。組織学的に見ると腫瘍は複数の種類の細胞から構成されており、軟骨組織、神経組織、筋肉組織、脂肪組織、および腸管様組織(図8)が認められたことから、iPS細胞の多能性が証明された。一方、3因子で樹立した細胞をヌードマウスに移植すると腫瘍は形成されたが、組織学的には未分化細胞からのみ形成されていた。したがって、分化多能性の誘導のためには、Soxファミリーが必須であることがわかった。
マウス胎児線維芽細胞(MEF)で同定した4因子をFbx15遺伝子にβgeoをノックインし、さらに全身で緑色蛍光蛋白質(GFP)を発現する成体マウスの尾部に由来する線維芽細胞に導入した。その後、フィーダー細胞上でES細胞培養条件と同様の条件で培養してG418による選択を行った。薬剤選択開始後約2週間で複数のiPS細胞コロニーが得られた。これらの細胞をヌードマウスの皮下に移植すると三胚葉系の様々な組織からなる奇形腫を形成した。また成体皮膚線維芽細胞に由来するiPS細胞を胚盤胞に移植し、偽妊娠マウスの子宮に移植したところ、受精後13.5日目の胚において、全身でGFP陽性細胞の分布しているものが認められた(図9)。これはiPS細胞が多能性を有しており、マウス胚発生に寄与できることを示している。この結果は、同定した因子群が胎児期の体細胞だけではなく成熟したマウスの体細胞に対しても初期化を誘導する能力のあることを示している。成体皮膚由来の細胞で初期化誘導できることは実用上極めて重要である。
iPS細胞樹立におけるサイトカインの影響を検討した。フィーダー細胞(STO細胞)に塩基性線維芽細胞増殖因子(bFGF)又は幹細胞因子(SCF)の発現ベクター(pMXレトロウイルスベクター)を導入し、これらのサイトカインを恒常的に発現する細胞を樹立した。Fbx15βgeo/βgeoマウス由来MEF(50万個/100mmディッシュ)をこれらのSTO細胞上で培養し、4因子を導入後G418 による選択を行ったところ、通常のSTO細胞上で培養した時と比べて、コロニー形成数がbFGF(図11)、SCF(data not shown)を産生するSTO細胞上では20倍以上上昇した。またc-Myc以外の3因子を導入しても通常のSTO細胞上ではiPS細胞コロニーは精製されなかったが、bFGF(図11)、SCF(data not shown)を産生するSTO細胞上では、コロニーの形成が認められた。これらの結果から、サイトカインの刺激により、MEFからのiPS細胞の樹立効率が上昇すること、及びc-Mycに換えてサイトカインを用いることにより核初期化が可能になることが明らかとなった。
Oct3/4、Klf4、c-Myc、及びSox2遺伝子にはすべてファミリー遺伝子(表1及び2)が存在する。そこで4遺伝子に換えてファミリー遺伝子によってもiPS細胞が樹立できるかを検討した。表7に2回の実験の結果を合わせたものを示す。Soxファミリーについては、Sox1はG418耐性コロニー数及びiPS細胞樹立効率とともにSox2と同程度であった。Sox3はG418耐性コロニー数はSox2の10分の1程度であったが、ひろったコロニーからのiPS細胞樹立効率はSox2よりむしろ高かった。Sox15はG418耐性コロニー数及びiPS細胞樹立効率とともにSox2より低かった。Sox17はG418耐性コロニーはSox2と同程度であったが、iPS細胞樹立効率は低かった。Klfファミリーについては、Klf2はKlf4より少ないG418耐性コロニーが生じたが、iPS細胞の樹立効率は同程度であった。Mycファミリーについては、まず野生型のc-MycがT58A変異体とG418耐性コロニー数、iPS細胞樹立効率の両者において同程度であることを確認した。さらにN-Myc及びL-Myc(ともに野生型)は、ともにc-MycとG418耐性コロニー数、iPS細胞樹立効率の両者において同程度であった。
Fbx15-βgeo以外のレポーターでiPS細胞が樹立できるかを検討した。まずNanog遺伝子を中央部に含む大腸菌人工染色体(BAC)を単離し、大腸菌内の組み換えにより、GFP遺伝子及びピューロマイシン耐性遺伝子をノックインした(図12A)。ついで同改変BACをES細胞に導入し、未分化状態特異的にGFP陽性となることを確認した(data not shown)。ついで同ES細胞のマウス胚盤胞に移植することによりキメラマウスを経てトランスジェニックマウスを作出した。このマウスにおいてはGFP陽性細胞は胚盤胞の内部細胞塊や受精後13.5日胚の生殖腺において特異的に認められた(図12B)。受精後13.5日胚(DBA、129及びC57BL/6マウスの雑種)から生殖腺を除去し、MEFを単離した。フローサイトメトリーにより、単離したMEFはGFP陰性であることを確認した(図13)。このMEFに4因子をレトロウイルスで導入し、ピューロマイシンによる選択を行ったところ、複数の耐性コロニーが得られた。その中の約10〜20%のみがGFP陽性であった。GFP陽性コロニーを継代培養するとES細胞に類似した形態(図14)や増殖(図15)を示した。また遺伝子発現を見るとFbx15βgeo/βgeoのMEFからG418選択により単離したiPS細胞より、さらにES細胞に近い発現パターンであることがわかった(図16)。この細胞をヌードマウスに移植すると奇形腫が形成されたことからiPS細胞であることが確認された(図17)。さらにNanog-GFP選択によるiPS細胞をC57BL/6マウスの胚盤胞に移植することによりキメラマウスが誕生した(図18)。さらにこのキメラマウス同士を交配させることによりジャームライントランスミッションが確認された(図19)。このNanog-GFP選択により樹立されたよりES細胞に近いiPS細胞においては、レトロウイルスからの4因子の発現はほぼ完全にサイレンシングを受けており、内在性のOct3/4やSox2により自己複製が維持されていることが示唆された。
10cm コンフルエントのiPS細胞を、トリプシン処理し、ES 細胞用培地に懸濁した(STO細胞は懸濁後10〜20分ゼラチンコートしたディッシュに接着させることによって除去した)。2×106の細胞を、HEMA(2-hydroxyethyl methacrylate)でコーティングした大腸菌培養用ディッシュで4日間浮遊培養し、Embryoid body(EB)を形成させた(day1-4)。EB形成4日目(day 4)に、EBを全量10cm組織培養用ディッシュに移しES 細胞用培地で24時間培養して接着させた。24時間後(day 5)にITS/fibronectin含有培地に交換した。7日間培養し(2日毎に培地交換を行う)、nestin 陽性細胞を選択した(無血清下で培養すると、他の系譜の細胞がある程度死んでいく)(day5-12)。次にA2B5陽性細胞の誘導を行った。7日後(day 12)にトリプシン処理して細胞をばらばらにし、残存するEBは除去した。1×105個の細胞をpoly-L-ornithine/fibronectinがコーティングされている 24ウェルプレートに撒き、N2/ bFGF 含有培地で四日間培養した(二日毎に培地交換(day12-16)。四日後(day 16)にN2/bFGF/EGF 含有培地へ交換し、四日間培養した(二日毎に培地交換)(day16-20)。四日後(day 20)にN2/bFGF/PDGF含有培地 に交換して、四日間培養した(二日毎に培地交換)(day20-24)。この期間(day12-24)に細胞が増えすぎてコンフルエントになった場合は随時継代し、1〜2×105個の細胞を撒いた。(継代時期によって数は変更する)。四日後(day 24)にN2/T3 培地 に交換して、7日間培養し(day24-31)し、2日毎に培地交換を行った。day 31に固定し、免疫染色した。その結果、iPS細胞から、βIIIチュブリン陽性の神経細胞、O4陽性のオリゴデンドロサイト、GFAP陽性のアストロサイトへの分化が確認された(図20)。
Fbx15-βgeoノックインマウス以外の任意のマウス体細胞からiPS細胞を樹立するために、薬剤選択を用いない樹立方法を開発した。10cmディッシュ(STOフィーダー細胞上)にマウス胎児線維芽細胞(MEF)を、これまでより少数(1万、5万又は10万個)培養し、レトロウイルスによりコントロールDNA又は4因子を導入した。ES細胞培地にて2週間培養(G418選択無し)行ったところ、コントロールDNAを導入したディッシュではコロニー形成が認められなかったが、4因子を導入したディッシュにおいては形質転換したと思われる扁平なコロニー加えて、複数のコンパクトなコロニーが形成された(図21)。これらから24コロニーをピックアップし培養を続けたところ、ES細胞様の形態が認められた。その遺伝子発現をRT-PCRにて検討したところ、7クローンにおいてES細胞マーカーであるEsg1の発現が認められた。またクローン4においてはNanog、ERas、GDF3、Oct3/4、Sox2などの多くのES細胞マーカーの誘導が認められたことからiPS細胞であると考えられた(図 22)。以上の結果より、iPS細胞樹立にはFbx15-βgeoノックインなどを用いた薬剤選択は必須でなく、任意のマウス由来体細胞からiPS細胞を樹立できることが示された。本技術により疾患モデルマウスの体細胞からもiPS細胞が樹立できる可能性が示された。
iPS細胞を誘導する細胞として、線維芽細胞以外の細胞である、肝細胞及び胃粘膜細胞を検討した。Fbx15βgeo/βgeoマウスの肝臓から肝細胞を還流により単離した。この肝細胞に4因子をレトロウイルスで投与し、G418による選択を行ったところ複数のiPS細胞コロニーが得られた。DNAマイクロアレーによる遺伝子発現パターン解析の結果、肝臓由来のiPS細胞は皮膚線維芽細胞や胎児線維芽細胞由来のiPS細胞より、さらにES細胞に類似していることが明らかとなった。胃粘膜細胞からも、肝細胞からと同様にiPS細胞が得られた。
PD98059はMAPキナーゼの阻害薬であり、多くの分化細胞においては増殖を抑制するが、ES細胞においては、未分化状態維持と増殖を促進することが知られている。そこでiPS細胞樹立におけるPD98059の効果を検討した。Nanog-EGFP-IRES-Puroの選択マーカーをもつマウスから樹立したMEFに4因子をレトロウイルスで投与し、ピューロマイシンによる選択を行った。PD98059を投与しない場合、得られたiPS細胞コロニーの中で、GFP陽性の割合は8%であった。一方、PD98059(最終濃度25μM)をレトロウイルス感染の翌日から持続的に投与した群では、得られたコロニーの45%がGFP陽性であった。これはPD98059がGFP陽性の、よりES細胞に近いiPS細胞の増殖を促進するが、GFP陰性のiPS細胞や、分化細胞の増殖は抑制するためであると考えられた。このことからPD98059は、よりES細胞に近いiPS細胞の樹立や、薬剤選択を用いないiPS細胞の樹立に利用できることが示された。
胎児由来のHuman dermal fibroblast (HDF) にマウスエコトロピックウイルスレセプターであるsolute carrier family 7(Slc7a1、NCBIアクセッション番号NM_007513)をレンチウイルスで発現させた細胞に、マウスOct3/4遺伝子プロモーター下流に赤色蛍光蛋白質遺伝子を、およびPGKプロモーター下流にハイグロマイシン耐性遺伝子を組み込んだプラスミドをヌクレオフェクションで導入した。ハイグロマイシンによる選択を行い、安定発現株を樹立した。800000個の細胞をマイトマイシン処理したSTO細胞の上にまき、翌日レトロウイルスによりOct3/4, Sox2, Klf4, c-Myc(いずれもヒト由来)を導入した。3週間後に得られたコロニー(図23左)を24個拾い、STO細胞を播種した24-well plateに移して培養した。2週間後に増えてきた1クローンをSTO細胞を播種した6-well plateに継代して培養した結果、ES細胞に形態上において類似した細胞が得られ(図23右)、iPS細胞であることが示唆された。培地は常にマウスES細胞用培地を用いた。
ヒト成体皮膚線維芽細胞(adult HDF) にレンチウイルスでSlc7a1(マウスレトロウイルス受容体)を導入した細胞を800000個のフィーダー細胞(マイトマイシン処理STO細胞)上にまき、以下の組み合わせでレトロウイルスにより遺伝子を導入した。
1. Oct3/4, Sox2, Klf4, c-Myc, TERT, SV40 Large T antigen
2. Oct3/4, Sox2, Klf4, c-Myc, TERT, HPV16 E6
3.Oct3/4, Sox2, Klf4, c-Myc, TERT, HPV16 E7
4. Oct3/4, Sox2, Klf4, c-Myc, TERT, HPV16 E6, HPV16 E7
5. Oct3/4, Sox2, Klf4, c-Myc, TERT, Bmi1
(Oct3/4, Sox2, Klf4, c-Myc, TERTはヒト由来、Bmi1はマウス由来)
マウスES細胞の培養条件下で、薬剤選択無しで培養を続けたところ、1の組み合わせで因子を導入したディッシュにおいて、ウイルス感染8日後において、iPS細胞と思われるコロニーが出現した(図24)。他の組み合わせ(2から5)においても、1の組み合わせの場合ほどは明瞭ではないが、iPS細胞様のコロニーが出現した。4因子のみを導入しても、全くコロニーは出現しなかった。
Claims (1)
- 体細胞から誘導多能性幹細胞を製造する方法であって、下記の4種の遺伝子:Oct3/4、Klf4、c-Myc、及びSox2を体細胞に導入する工程を含む方法。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2008131577A JP4183742B1 (ja) | 2005-12-13 | 2008-05-20 | 誘導多能性幹細胞の製造方法 |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2005359537 | 2005-12-13 | ||
JP2008131577A JP4183742B1 (ja) | 2005-12-13 | 2008-05-20 | 誘導多能性幹細胞の製造方法 |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2007550210A Division JP5098028B2 (ja) | 2005-12-13 | 2006-12-06 | 核初期化因子 |
Publications (2)
Publication Number | Publication Date |
---|---|
JP4183742B1 true JP4183742B1 (ja) | 2008-11-19 |
JP2008283972A JP2008283972A (ja) | 2008-11-27 |
Family
ID=38162968
Family Applications (8)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2007550210A Active JP5098028B2 (ja) | 2005-12-13 | 2006-12-06 | 核初期化因子 |
JP2008131577A Active JP4183742B1 (ja) | 2005-12-13 | 2008-05-20 | 誘導多能性幹細胞の製造方法 |
JP2009056747A Active JP4411362B2 (ja) | 2005-12-13 | 2009-03-10 | 誘導多能性幹細胞の製造方法 |
JP2009056748A Active JP5248371B2 (ja) | 2005-12-13 | 2009-03-10 | 誘導多能性幹細胞を製造するための核初期化因子の使用 |
JP2009056750A Active JP4411363B2 (ja) | 2005-12-13 | 2009-03-10 | 誘導多能性幹細胞からの体細胞の製造方法 |
JP2009056749A Active JP5467223B2 (ja) | 2005-12-13 | 2009-03-10 | 誘導多能性幹細胞およびその製造方法 |
JP2011088113A Active JP5603282B2 (ja) | 2005-12-13 | 2011-04-12 | 誘導多能性幹細胞 |
JP2013167725A Active JP5943324B2 (ja) | 2005-12-13 | 2013-08-12 | 誘導多能性幹細胞 |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2007550210A Active JP5098028B2 (ja) | 2005-12-13 | 2006-12-06 | 核初期化因子 |
Family Applications After (6)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2009056747A Active JP4411362B2 (ja) | 2005-12-13 | 2009-03-10 | 誘導多能性幹細胞の製造方法 |
JP2009056748A Active JP5248371B2 (ja) | 2005-12-13 | 2009-03-10 | 誘導多能性幹細胞を製造するための核初期化因子の使用 |
JP2009056750A Active JP4411363B2 (ja) | 2005-12-13 | 2009-03-10 | 誘導多能性幹細胞からの体細胞の製造方法 |
JP2009056749A Active JP5467223B2 (ja) | 2005-12-13 | 2009-03-10 | 誘導多能性幹細胞およびその製造方法 |
JP2011088113A Active JP5603282B2 (ja) | 2005-12-13 | 2011-04-12 | 誘導多能性幹細胞 |
JP2013167725A Active JP5943324B2 (ja) | 2005-12-13 | 2013-08-12 | 誘導多能性幹細胞 |
Country Status (18)
Country | Link |
---|---|
US (1) | US8048999B2 (ja) |
EP (6) | EP1970446B1 (ja) |
JP (8) | JP5098028B2 (ja) |
KR (1) | KR101420740B1 (ja) |
CN (4) | CN103113463B (ja) |
AU (1) | AU2006325975B2 (ja) |
BR (1) | BRPI0619794B8 (ja) |
CA (1) | CA2632142C (ja) |
DK (1) | DK1970446T3 (ja) |
EA (2) | EA014166B1 (ja) |
ES (1) | ES2367525T3 (ja) |
HK (2) | HK1125131A1 (ja) |
IL (1) | IL191903A (ja) |
MX (2) | MX2008007654A (ja) |
NZ (1) | NZ569530A (ja) |
PT (1) | PT1970446E (ja) |
WO (1) | WO2007069666A1 (ja) |
ZA (1) | ZA200804673B (ja) |
Cited By (33)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2010119819A1 (ja) | 2009-04-17 | 2010-10-21 | 国立大学法人東北大学 | ヒト肺組織幹細胞の調製方法及びヒト肺胞上皮細胞への分化誘導方法 |
WO2011024550A1 (ja) * | 2009-08-31 | 2011-03-03 | 国立大学法人大阪大学 | 口腔粘膜由来細胞を利用した誘導多能性幹細胞の効率的な製造方法 |
WO2014027684A1 (ja) | 2012-08-17 | 2014-02-20 | 株式会社Clio | 心筋梗塞の修復再生を誘導する多能性幹細胞 |
WO2014133170A1 (ja) | 2013-03-01 | 2014-09-04 | 株式会社Clio | 多能性幹細胞を損傷部位に誘導する遊走因子を含む医薬組成物 |
KR20150063168A (ko) | 2009-07-15 | 2015-06-08 | 마리 데자와 | 생체조직으로부터 단리 할 수 있는 다능성 줄기세포 |
WO2017040548A1 (en) | 2015-08-31 | 2017-03-09 | I Peace, Inc. | Pluripotent stem cell manufacturing system and method for producing induced pluripotent stem cells |
KR20170055477A (ko) | 2014-09-05 | 2017-05-19 | 고쿠리츠다이가쿠호우진 도쿄다이가쿠 | 당뇨병성 피부 궤양 치료를 위한 다능성 줄기 세포 |
DE102017212851A1 (de) | 2016-08-04 | 2018-02-08 | Fanuc Corporation | Stammzellenherstellungssystem, Stammzelleninformationsverwaltungssystem, Zellentransportvorrichtung und Speichervorrichtung für gefrorene Stammzellen |
WO2018154791A1 (ja) | 2017-02-27 | 2018-08-30 | 剛士 田邊 | 細胞処理システム及び細胞処理装置 |
WO2018155607A1 (ja) | 2017-02-24 | 2018-08-30 | 剛士 田邊 | 細胞処理装置、浮遊培養器、及び幹細胞の誘導方法 |
WO2018154788A1 (ja) | 2017-02-27 | 2018-08-30 | 剛士 田邊 | 体細胞製造システム |
WO2018203499A1 (ja) | 2017-05-02 | 2018-11-08 | 剛士 田邊 | 医薬品組成物及び化粧品組成物 |
WO2018235878A1 (ja) | 2017-06-20 | 2018-12-27 | 国立大学法人名古屋大学 | 多能性幹細胞による胎児発育不全に伴う脳障害の改善及び治療 |
KR20190039102A (ko) | 2016-08-03 | 2019-04-10 | 고쿠리츠 다이가쿠 호우징 나고야 다이가쿠 | 다능성 간세포에 따른 만성폐질환의 개선 및 치료 |
KR20190039700A (ko) | 2016-08-03 | 2019-04-15 | 가부시키가이샤 세이메이카가쿠 인스티튜트 | 다능성 간세포에 의한 허혈재관류 폐장애의 경감 및 치료 |
WO2019078262A1 (ja) | 2017-10-17 | 2019-04-25 | 国立大学法人広島大学 | 骨軟骨修復を誘導する多能性幹細胞 |
WO2020040118A1 (ja) | 2018-08-20 | 2020-02-27 | アイ ピース, インコーポレイテッド | 細胞培養器 |
WO2020040135A1 (ja) | 2018-08-20 | 2020-02-27 | 剛士 田邊 | 細胞の培養又は誘導方法 |
WO2020179127A1 (ja) | 2019-03-05 | 2020-09-10 | ファナック株式会社 | 細胞製造システム |
KR20200127039A (ko) | 2016-05-16 | 2020-11-09 | 고쿠리츠 다이가쿠 호우징 도우카이 고쿠리츠 다이가쿠 기코우 | 다능성 간세포에 의한 주산기 뇌장애의 개선 및 치료 |
WO2020250929A1 (ja) | 2019-06-10 | 2020-12-17 | アイ ピース, インコーポレイテッド | 赤血球除去装置、単核球回収器、細胞培養装置、細胞培養システム、細胞培養方法、及び単核球の回収方法 |
WO2020250927A1 (ja) | 2019-06-10 | 2020-12-17 | アイ ピース, インコーポレイテッド | 赤血球除去装置、単核球回収器、細胞培養装置、細胞培養システム、細胞培養方法、及び単核球の回収方法 |
WO2020262354A1 (ja) | 2019-06-28 | 2020-12-30 | アイ ピース, インコーポレイテッド | 細胞培養器及び細胞培養装置 |
WO2020262351A1 (ja) | 2019-06-28 | 2020-12-30 | アイ ピース, インコーポレイテッド | 細胞塊分割器、細胞塊分割器の製造方法、及び細胞塊の分割方法 |
WO2021038998A1 (ja) | 2019-08-29 | 2021-03-04 | ファナック株式会社 | 細胞製造装置及びそのシステム |
WO2021038996A1 (ja) | 2019-08-29 | 2021-03-04 | ファナック株式会社 | 細胞製造装置及びその製造方法 |
WO2021038997A1 (ja) | 2019-08-29 | 2021-03-04 | ファナック株式会社 | 細胞製造装置 |
WO2021085639A1 (ja) | 2019-10-31 | 2021-05-06 | 株式会社生命科学インスティテュート | 多能性幹細胞による間質性膀胱炎の治療 |
WO2021090767A1 (ja) | 2019-11-06 | 2021-05-14 | アイ ピース, インコーポレイテッド | 細胞培養装置 |
WO2021186648A1 (ja) | 2020-03-18 | 2021-09-23 | ファナック株式会社 | 顕微鏡観察システム |
WO2022050419A1 (ja) | 2020-09-04 | 2022-03-10 | Heartseed株式会社 | iPS細胞の品質改善剤、iPS細胞の製造方法、iPS細胞、及びiPS細胞製造用組成物 |
EP4019620A1 (en) | 2020-12-22 | 2022-06-29 | I Peace, Inc. | Cell culture vessel and method for culturing cell |
US12036245B2 (en) | 2018-11-07 | 2024-07-16 | I Peace, Inc. | Pharmaceutical composition and cosmetic composition |
Families Citing this family (548)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ITRM20030376A1 (it) | 2003-07-31 | 2005-02-01 | Univ Roma | Procedimento per l'isolamento e l'espansione di cellule staminali cardiache da biopsia. |
US7682828B2 (en) | 2003-11-26 | 2010-03-23 | Whitehead Institute For Biomedical Research | Methods for reprogramming somatic cells |
ES2701704T3 (es) | 2004-04-07 | 2019-02-25 | Ncardia Ag | Sistemas de ensayo tisulares funcionales no invasivos in vitro |
WO2005108598A1 (en) | 2004-05-11 | 2005-11-17 | Axiogenesis Ag | Assay for drug discovery based on in vitro differentiated cells |
US11660317B2 (en) | 2004-11-08 | 2023-05-30 | The Johns Hopkins University | Compositions comprising cardiosphere-derived cells for use in cell therapy |
ES2933478T3 (es) * | 2005-08-03 | 2023-02-09 | Astellas Inst For Regenerative Medicine | Métodos mejorados de reprogramación de células somáticas animales |
US9012219B2 (en) * | 2005-08-23 | 2015-04-21 | The Trustees Of The University Of Pennsylvania | RNA preparations comprising purified modified RNA for reprogramming cells |
US10646590B2 (en) | 2005-12-13 | 2020-05-12 | The Trustees Of The University Of Pennsylvania | Methods for phototransfecting nucleic acids into live cells |
US8129187B2 (en) * | 2005-12-13 | 2012-03-06 | Kyoto University | Somatic cell reprogramming by retroviral vectors encoding Oct3/4. Klf4, c-Myc and Sox2 |
US9157066B2 (en) | 2005-12-13 | 2015-10-13 | The Trustees Of The University Of Pennsylvania | Transcriptome transfer produces cellular phenotype conversion |
US8278104B2 (en) * | 2005-12-13 | 2012-10-02 | Kyoto University | Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2 |
US20090227032A1 (en) * | 2005-12-13 | 2009-09-10 | Kyoto University | Nuclear reprogramming factor and induced pluripotent stem cells |
EP1970446B1 (en) | 2005-12-13 | 2011-08-03 | Kyoto University | Nuclear reprogramming factor |
US10647960B2 (en) * | 2005-12-13 | 2020-05-12 | The Trustees Of The University Of Pennsylvania | Transcriptome transfer produces cellular phenotype conversion |
EP1994144B1 (en) | 2006-03-06 | 2017-11-22 | Agency for Science, Technology and Research | Human embryonic stem cell methods and podxl expression |
CN101679950A (zh) * | 2007-02-22 | 2010-03-24 | 国立大学法人东京大学 | 利用胚泡互补的器官再生法 |
SG193653A1 (en) * | 2007-03-23 | 2013-10-30 | Wisconsin Alumni Res Found | Somatic cell reprogramming |
AU2016216711B2 (en) * | 2007-04-07 | 2018-01-25 | Whitehead Institute For Biomedical Research | Reprogramming of somatic cells |
EP2145000A4 (en) * | 2007-04-07 | 2010-05-05 | Whitehead Biomedical Inst | REPROGRAMMING SOMATIC CELLS |
SG10201903161XA (en) * | 2007-05-29 | 2019-05-30 | Christopher Reid | Methods for production and uses of multipotent cell populations |
US9213999B2 (en) | 2007-06-15 | 2015-12-15 | Kyoto University | Providing iPSCs to a customer |
JP2008307007A (ja) * | 2007-06-15 | 2008-12-25 | Bayer Schering Pharma Ag | 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞 |
US9080145B2 (en) | 2007-07-01 | 2015-07-14 | Lifescan Corporation | Single pluripotent stem cell culture |
MX2010001335A (es) | 2007-07-31 | 2010-06-02 | Lifescan Inc | Diferenciacion de celulas madre embrionarias humanas. |
WO2009032456A2 (en) * | 2007-08-01 | 2009-03-12 | Primegen Biotech Llc | Non-viral delivery of transcription factors that reprogram human somatic cells into a stem cell-like state |
CN101855338B (zh) | 2007-08-31 | 2013-07-17 | 怀特黑德生物医学研究所 | 在程序重排体细胞中的wnt途径刺激 |
EP2096169B1 (en) * | 2007-10-31 | 2020-11-18 | Kyoto University | Nuclear reprogramming method |
BRPI0819609A2 (pt) | 2007-11-27 | 2014-10-14 | Lifescan Inc | Diferenciação de células-tronco embrionárias de ser humano |
WO2009073523A2 (en) * | 2007-11-29 | 2009-06-11 | Children's Hospital Of Orange County | De-differentiation of human cells |
JP5626619B2 (ja) * | 2008-12-08 | 2014-11-19 | 国立大学法人京都大学 | 効率的な核初期化方法 |
US9683232B2 (en) * | 2007-12-10 | 2017-06-20 | Kyoto University | Efficient method for nuclear reprogramming |
JP5558097B2 (ja) * | 2007-12-10 | 2014-07-23 | 国立大学法人京都大学 | 効率的な核初期化方法 |
US8609413B2 (en) | 2007-12-11 | 2013-12-17 | Research Development Foundation | Neurons, astrocytes and oligodendrocytes differentiated from a mammalian pluripotent or neural stem cells exposed to a pyridine deriviative |
EP2072618A1 (en) * | 2007-12-14 | 2009-06-24 | Johannes Gutenberg-Universität Mainz | Use of RNA for reprogramming somatic cells |
KR101481164B1 (ko) * | 2008-01-30 | 2015-01-09 | 주식회사 미래셀바이오 | 체세포 유래 다능성 줄기세포의 제조 방법 |
WO2009096049A1 (ja) * | 2008-02-01 | 2009-08-06 | Kyoto University | 人工多能性幹細胞由来分化細胞 |
AU2009215516B2 (en) | 2008-02-21 | 2014-12-18 | Janssen Biotech Inc. | Methods, surface modified plates and compositions for cell attachment, cultivation and detachment |
WO2009104794A1 (ja) * | 2008-02-22 | 2009-08-27 | 国立大学法人 東京大学 | 遺伝子改変による致死性表現型を持つ動物の繁殖用ファウンダー動物作製法 |
JP2009215191A (ja) | 2008-03-07 | 2009-09-24 | Keio Gijuku | 神経損傷治療剤及び神経損傷治療方法 |
EP2100954A1 (en) * | 2008-03-10 | 2009-09-16 | Assistance Publique - Hopitaux de Paris | Method for generating primate cardiac progenitor cells for clinical use from primate embryonic stem cells, and their applications |
RU2010139426A (ru) * | 2008-03-17 | 2012-04-27 | Зе Скрипс Ресеч Инститьют (Us) | Комбинация химического и генетического подходов к получению индуцированных плюрипотентных стволовых клеток |
AU2015201026B2 (en) * | 2008-03-17 | 2017-03-16 | The Scripps Research Institute | Combined chemical and genetic approaches for generation of induced pluripotent stem cells |
WO2009114949A1 (en) * | 2008-03-20 | 2009-09-24 | UNIVERSITé LAVAL | Methods for deprogramming somatic cells and uses thereof |
WO2009118928A1 (en) | 2008-03-26 | 2009-10-01 | Kyoto University | Efficient production and use of highly cardiogenic progenitors and cardiomyocytes from embryonic and induced pluripotent stem cells |
WO2009119105A1 (ja) * | 2008-03-28 | 2009-10-01 | 国立大学法人東京大学 | GPIbα+GPV+GPVI+血小板のインビトロ調製法 |
US20110117645A1 (en) * | 2008-03-31 | 2011-05-19 | Oriental Yeast Co., Ltd. | Method for proliferation of pluripotent stem cells |
US8546141B2 (en) | 2008-04-01 | 2013-10-01 | The University Of Tokyo | Method for preparation of platelet from iPS cell |
WO2009146098A2 (en) * | 2008-04-02 | 2009-12-03 | President And Fellows Of Harvard College | Stem cells and uses thereof |
US20100021437A1 (en) * | 2008-04-07 | 2010-01-28 | The McLean Hospital Corporation Whitehead Institute for Biomedical Research | Neural stem cells derived from induced pluripotent stem cells |
JP2011522514A (ja) * | 2008-04-07 | 2011-08-04 | ニューポテンシャル,インコーポレイテッド | Rna干渉を介する多能性遺伝子の誘発による細胞の再プログラミング |
US8623648B2 (en) * | 2008-04-24 | 2014-01-07 | Janssen Biotech, Inc. | Treatment of pluripotent cells |
KR101481165B1 (ko) * | 2008-04-25 | 2015-01-09 | 주식회사 미래셀바이오 | 인간 체세포 유래 다능성 줄기세포의 제조 방법 |
EP2268809B1 (en) * | 2008-05-02 | 2019-02-06 | Kyoto University | Method of nuclear reprogramming |
JP2011522520A (ja) * | 2008-05-06 | 2011-08-04 | エージェンシー フォー サイエンス,テクノロジー アンド リサーチ | 細胞の脱分化を行う方法 |
EP2297298A4 (en) * | 2008-05-09 | 2011-10-05 | Vistagen Therapeutics Inc | PANCREATIC ENDOCRINE PROGENITOR CELLS FROM PLURIPOTENT STEM CELLS |
WO2009142717A2 (en) * | 2008-05-19 | 2009-11-26 | President And Fellows Of Harvard College | Methods and products for dedifferentiation of cells |
EP2128245A1 (en) * | 2008-05-27 | 2009-12-02 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Generation of induced pluripotent stem (iPS) cells |
JP2011160661A (ja) * | 2008-06-02 | 2011-08-25 | Kyowa Hakko Kirin Co Ltd | 血球細胞の初期化法 |
US8546140B2 (en) * | 2008-06-04 | 2013-10-01 | Cellular Dynamics International, Inc. | Methods for the production of iPS cells using non-viral approach |
AU2015202237B2 (en) * | 2008-06-13 | 2017-09-28 | Whitehead Institute For Biomedical Research | Programming and reprogramming of cells |
CN105671065A (zh) * | 2008-06-13 | 2016-06-15 | 怀特黑德生物医学研究所 | 细胞编程和重编程 |
US8669048B2 (en) | 2008-06-24 | 2014-03-11 | Parkinson's Institute | Pluripotent cell lines and methods of use thereof |
WO2009157610A1 (en) * | 2008-06-26 | 2009-12-30 | Pusan National University Industry-University Cooperation Foundation | Selenium dedifferentiated cell, preparation method and usage thereof |
WO2009157201A1 (en) * | 2008-06-26 | 2009-12-30 | Osaka University | Method and kit for preparing ips cells |
EP2288692B1 (en) | 2008-06-27 | 2017-08-02 | Kyoto University | Method of efficiently establishing induced pluripotent stem cells |
KR20180018839A (ko) | 2008-06-30 | 2018-02-21 | 얀센 바이오테크 인코포레이티드 | 만능 줄기 세포의 분화 |
WO2010008054A1 (ja) | 2008-07-16 | 2010-01-21 | ディナベック株式会社 | 染色体非組み込み型ウイルスベクターを用いてリプログラムされた細胞を製造する方法 |
CA2697621C (en) | 2008-07-30 | 2017-01-17 | Kyoto University | Method of efficiently establishing induced pluripotent stem cells |
WO2010013359A1 (en) * | 2008-07-31 | 2010-02-04 | Gifu University | Efficient method for establishing induced pluripotent stem cells |
JP5671737B2 (ja) | 2008-08-05 | 2015-02-18 | 学校法人慶應義塾 | 分化細胞由来多能性幹細胞由来の二次ニューロスフェアの選択方法、その選択方法によって選択されたクローン、及びそのクローンの使用方法 |
WO2010017562A2 (en) | 2008-08-08 | 2010-02-11 | Mayo Foundation For Medical Education And Research | Induced pluripotent stem cells |
JP2012500005A (ja) * | 2008-08-12 | 2012-01-05 | セルラー ダイナミクス インターナショナル, インコーポレイテッド | iPS細胞を生成するための方法 |
CA2734859A1 (en) * | 2008-08-21 | 2010-02-25 | Richter Gedeon Nyrt. | Methods for treating cns disorders |
CN102196722A (zh) * | 2008-08-22 | 2011-09-21 | 国立大学法人东京大学 | 利用iPS细胞和胚泡互补的器官再生法 |
JP5709751B2 (ja) * | 2008-09-04 | 2015-04-30 | エイビーティー ホールディング カンパニー | ニューロンの軸索退縮を予防するための幹細胞の使用 |
WO2010027062A1 (ja) | 2008-09-04 | 2010-03-11 | 独立行政法人理化学研究所 | B細胞由来iPS細胞およびその用途 |
ES2549155T3 (es) * | 2008-09-08 | 2015-10-23 | Riken | Células iPS obtenidas a partir de células NKT y células NKT obtenidas a partir de las mismas |
CA2736877A1 (en) * | 2008-09-12 | 2010-03-18 | Scarab Genomics, Llc | Clean genome bactofection |
CN101492676B (zh) * | 2008-09-16 | 2011-02-16 | 中国科学院广州生物医药与健康研究院 | 用脑膜细胞生成诱导的多能性干细胞的方法及其用途 |
SG160248A1 (en) * | 2008-09-18 | 2010-04-29 | Agency Science Tech & Res | Use of novel monoclonal antibodies targeting human embryonic stem cells to characterize and kill induced pluripotent stem cells |
US8703413B2 (en) | 2008-09-22 | 2014-04-22 | Children's Medical Center Corporation | Detection of human somatic cell reprogramming |
JP5676265B2 (ja) * | 2008-10-24 | 2015-02-25 | 株式会社クラレ | 細胞保存方法、及び細胞輸送方法 |
WO2010050626A1 (en) * | 2008-10-30 | 2010-05-06 | Kyoto University | Method for producing induced pluripotent stem cells |
BRPI0919885A2 (pt) | 2008-10-31 | 2015-08-11 | Centocor Ortho Biotech Inc | Diferenciação de células-tronco embrionárias humanas para a linhagem endócrina pancreática |
CN102333862B (zh) | 2008-10-31 | 2018-04-27 | 詹森生物科技公司 | 人胚胎干细胞向胰腺内分泌谱系的分化 |
EP2356223B1 (en) | 2008-11-05 | 2016-04-20 | Keio University | Method for producing neural stem cells |
KR101687344B1 (ko) | 2008-11-20 | 2016-12-16 | 얀센 바이오테크 인코포레이티드 | 평면 기재상의 세포 부착 및 배양을 위한 방법 및 조성물 |
MX356756B (es) | 2008-11-20 | 2018-06-11 | Centocor Ortho Biotech Inc | Células madre pluripotentes en microportadores. |
EP2881461A1 (en) * | 2008-11-21 | 2015-06-10 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Reprogramming cells toward a pluripotent state |
NO2373784T3 (ja) * | 2008-12-17 | 2018-03-24 | ||
CA2688804A1 (en) | 2008-12-17 | 2010-06-17 | The Uab Research Foundation | Polycistronic vector for human induced pluripotent stem cell production |
EP2377926A4 (en) * | 2008-12-18 | 2011-12-14 | New Ind Res Organization | CHONDROCYTE TYPE CELL, AND PROCESS FOR PRODUCING THE SAME |
US8470308B2 (en) * | 2009-01-03 | 2013-06-25 | Ray C. Wasielewski | Enhanced medical implant comprising disrupted tooth pulp and tooth particles |
US10328103B2 (en) | 2009-01-03 | 2019-06-25 | Ray C. Wasielewski | Medical treatment composition comprising mammalian dental pulp stem cells |
US10041040B2 (en) | 2009-02-03 | 2018-08-07 | Keio University | Culture method of embryoid bodies and/or neural stem cells derived from human differentiated cell-derived pluripotent stem cells |
WO2010093655A2 (en) * | 2009-02-10 | 2010-08-19 | University Of Dayton | Enhanced method for producing stem-like cells from somatic cells |
WO2010098419A1 (en) * | 2009-02-27 | 2010-09-02 | Kyoto University | Novel nuclear reprogramming substance |
CA2755870C (en) | 2009-03-20 | 2019-04-09 | Angioblast Systems, Inc. | Production of reprogrammed pluripotent cells |
JP5637354B2 (ja) * | 2009-03-30 | 2014-12-10 | 独立行政法人産業技術総合研究所 | 精製転写因子の調製法と細胞導入技術 |
CN101613717B (zh) * | 2009-04-17 | 2012-01-11 | 中国科学院广州生物医药与健康研究院 | 用猪成纤维细胞生成诱导的多能性干细胞的方法 |
CN101580816B (zh) * | 2009-04-23 | 2012-02-29 | 中国科学院广州生物医药与健康研究院 | 诱导多能性干细胞快速高效产生的新型无血清培养基以及使用其的方法 |
JP2010268789A (ja) | 2009-04-24 | 2010-12-02 | Kumamoto Univ | 細胞医薬の製造方法 |
EP2253700A1 (en) | 2009-05-13 | 2010-11-24 | Helmholtz-Zentrum für Infektionsforschung GmbH | A method for producing test systems from donors suffering from adverse effects of medicaments and /or medical treatments, and uses of said systems |
US8957037B2 (en) | 2009-05-18 | 2015-02-17 | Curna, Inc. | Treatment of reprogramming factor related diseases by inhibition of natural antisense transcript to a reprogramming factor |
US9045738B2 (en) | 2009-05-29 | 2015-06-02 | Kyoto University | Method for producing induced pluripotent stem cells and method for culturing the same |
WO2010137348A1 (en) | 2009-05-29 | 2010-12-02 | Keio University | Method for selecting clone of induced pluripotent stem cells |
US9365866B2 (en) | 2009-06-03 | 2016-06-14 | National Institute Of Advanced Industrial Science And Technology | Vectors for generating pluripotent stem cells and methods of producing pluripotent stem cells using the same |
US20110002897A1 (en) * | 2009-06-11 | 2011-01-06 | Burnham Institute For Medical Research | Directed differentiation of stem cells |
US9399758B2 (en) | 2009-07-15 | 2016-07-26 | Mari Dezawa | SSEA3(+) pluripotent stem cell that can be isolated from body tissue |
KR20170118969A (ko) | 2009-07-20 | 2017-10-25 | 얀센 바이오테크 인코포레이티드 | 인간 배아 줄기 세포의 분화 |
CN102471744B (zh) * | 2009-07-21 | 2015-06-10 | 国立大学法人京都大学 | 图像处理装置、培养观察装置及图像处理方法 |
SG10201608797WA (en) * | 2009-08-07 | 2016-12-29 | Univ Kyoto | Method of efficiently establishing induced pluripotent stem cells |
JP5751548B2 (ja) | 2009-08-07 | 2015-07-22 | 国立大学法人京都大学 | イヌiPS細胞及びその製造方法 |
CN101993495B (zh) * | 2009-08-12 | 2013-07-24 | 上海近岸科技有限公司 | 一种蛋白质混合物及其制备方法 |
ES2590036T3 (es) | 2009-08-12 | 2016-11-17 | Kyoto University | Método para inducir la diferenciación de células madre pluripotentes en células precursoras neurales |
WO2011021706A1 (ja) | 2009-08-19 | 2011-02-24 | 国立大学法人東北大学 | 角膜移植用シート |
AU2010286740B2 (en) | 2009-08-22 | 2016-03-10 | The Board Of Trustees Of The Leland Stanford Junior University | Imaging and evaluating embryos, oocytes, and stem cells |
US20110052549A1 (en) * | 2009-08-27 | 2011-03-03 | The Regents Of The University Of California | Cell culture device to differentiate stem cells in a specific orientation |
EP2473598B1 (en) | 2009-09-04 | 2017-03-22 | The U.S.A. As Represented By The Secretary, Department Of Health And Human Services | Methods for enhancing genome stability and telomere elongation in embryonic stem cells |
GB0915523D0 (en) * | 2009-09-07 | 2009-10-07 | Genome Res Ltd | Cells and methods for obtaining them |
US8741644B2 (en) | 2009-09-08 | 2014-06-03 | Kyoto University | Method for producing mast cells from pluripotent stem cells |
US20120263689A1 (en) * | 2009-09-10 | 2012-10-18 | The Salk Institute For Biological Studies | Adipose-derived induced pluripotent stem cells |
AU2014240253B2 (en) * | 2009-09-15 | 2017-08-03 | The University Of Tokyo | Novel Method for Producing Differentiated Cells |
ES2725688T3 (es) * | 2009-09-15 | 2019-09-26 | Univ Tokyo | Método novedoso para la producción de células diferenciadas |
JP5773393B2 (ja) | 2009-09-24 | 2015-09-02 | 国立大学法人京都大学 | 効率的な人工多能性幹細胞の樹立方法 |
JP5880868B2 (ja) * | 2009-09-30 | 2016-03-09 | エージェンシー フォー サイエンス,テクノロジー アンド リサーチ | 核内受容体及びその変異体、並びに細胞の再プログラミングにおけるその使用 |
CA3194845A1 (en) | 2009-10-16 | 2011-04-21 | The Scripps Research Institute | Induction of pluripotent cells |
WO2011050470A1 (en) * | 2009-10-29 | 2011-05-05 | Mcmaster University | Generating induced pluripotent stem cells and progenitor cells from fibroblasts |
GB0919773D0 (en) | 2009-11-12 | 2009-12-30 | Univ Nottingham | Induced pluripotent stem cell |
DK3633025T3 (da) | 2009-11-12 | 2022-12-12 | Technion Res & Dev Foundation | Dyrkningsmedier, cellekulturer og metoder til dyrkning af pluripotente stamceller i en udifferentieret tilstand |
US8716020B2 (en) | 2009-11-13 | 2014-05-06 | Inserm (Institut National De La Sante Et De La Recherche Medicale) | Reprogrammation of eukaryotic cells with engineered microvesicles |
WO2011062559A1 (en) | 2009-11-19 | 2011-05-26 | Agency For Science, Technology And Research | Methods of enhancing pluripotentcy |
WO2011071118A1 (ja) | 2009-12-09 | 2011-06-16 | 国立大学法人京都大学 | ニトロビンを含む多能性幹細胞の心筋細胞への分化促進剤 |
WO2011074690A1 (en) | 2009-12-14 | 2011-06-23 | Kyoto University | Pharmaceutical composition for prevention and treatment of amyotrophic lateral sclerosis |
EP2516625B1 (en) | 2009-12-23 | 2024-06-26 | Janssen Biotech, Inc. | Differentiation of human embryonic stem cells |
JP2011135864A (ja) * | 2009-12-30 | 2011-07-14 | Korea Univ Research & Business Foundation | Oct4及びBmi1、またはその上位調節子を用いて体細胞から胚幹細胞類似細胞への逆分化を誘導する組成物及びこれを用いた胚幹細胞類似細胞の製造方法 |
CN102791857B (zh) | 2010-01-06 | 2017-03-15 | 国立大学法人鸟取大学 | 小鼠人工染色体载体 |
EP2526189B1 (en) | 2010-01-22 | 2016-10-12 | Kyoto University | Method for improving induced pluripotent stem cell generation efficiency |
EP2532741B1 (en) | 2010-02-03 | 2018-04-04 | Tetsuya Ishikawa | Induced hepatic stem cell and process for production thereof, and applications of the cell |
US8927277B2 (en) | 2010-02-16 | 2015-01-06 | Kyoto University | Method of efficiently establishing induced pluripotent stem cells |
JP5840119B2 (ja) | 2010-02-18 | 2016-01-06 | 国立大学法人大阪大学 | 誘導多能性幹細胞の製造方法 |
AU2011223900A1 (en) | 2010-03-01 | 2012-09-13 | Janssen Biotech, Inc. | Methods for purifying cells derived from pluripotent stem cells |
WO2011109026A1 (en) | 2010-03-05 | 2011-09-09 | Tissue Genesis, Inc. | Methods and compositions to support tissue integration and inosculation of transplanted tissue and transplanted engineered penile tissue with adipose stromal cells |
EP2545163A4 (en) * | 2010-03-10 | 2013-11-06 | Univ Kyoto | METHOD FOR SELECTION OF INDUCED PLURIPOTENTAL STEM CELLS |
AU2011235212B2 (en) | 2010-03-31 | 2014-07-31 | The Scripps Research Institute | Reprogramming cells |
CN103097521A (zh) | 2010-04-16 | 2013-05-08 | 学校法人庆应义塾 | 人工多能性干细胞的制造方法 |
US8815592B2 (en) | 2010-04-21 | 2014-08-26 | Research Development Foundation | Methods and compositions related to dopaminergic neuronal cells |
US9845457B2 (en) | 2010-04-30 | 2017-12-19 | Cedars-Sinai Medical Center | Maintenance of genomic stability in cultured stem cells |
US9249392B2 (en) | 2010-04-30 | 2016-02-02 | Cedars-Sinai Medical Center | Methods and compositions for maintaining genomic stability in cultured stem cells |
CN102242146B (zh) * | 2010-05-10 | 2015-11-25 | 高丽大学校产学协力团 | 组合物和用其产生诱导全能干细胞的方法 |
CN102884176B (zh) | 2010-05-12 | 2017-09-05 | 詹森生物科技公司 | 人胚胎干细胞的分化 |
CA2804119A1 (en) | 2010-05-25 | 2011-12-01 | National Cancer Center | Induced malignant stem cells or pre-induction cancer stem cells capable of self-replication outside of an organism, production method for same, and practical application for same |
EP3399026B1 (en) | 2010-06-14 | 2024-06-26 | The Scripps Research Institute | Reprogramming of cells to a new fate |
US8932857B2 (en) | 2010-06-15 | 2015-01-13 | Kyoto University | Method for selecting reduced differentiation resistance human induced pluripotent stem cells |
EP2582792B1 (en) * | 2010-06-18 | 2018-10-31 | FUJIFILM Cellular Dynamics, Inc. | Cardiomyocyte medium with dialyzed serum |
US9476041B2 (en) | 2010-07-12 | 2016-10-25 | National University Corporation Tottori University | Method for producing novel hipsc by means of siRNA introduction |
WO2012011610A1 (en) | 2010-07-21 | 2012-01-26 | Kyoto University | Method for inducing differentiation of human pluripotent stem cell into intermediate mesoderm cell |
EP3578205A1 (en) | 2010-08-06 | 2019-12-11 | ModernaTX, Inc. | A pharmaceutical formulation comprising engineered nucleic acids and medical use thereof |
US9938496B2 (en) | 2010-08-13 | 2018-04-10 | Kyoto University | Method of inducing differentiation from pluripotent stem cells to germ cells |
WO2012027358A1 (en) | 2010-08-23 | 2012-03-01 | President And Fellows Of Harvard College | Optogenetic probes for measuring membrane potential |
US9499790B2 (en) | 2010-08-26 | 2016-11-22 | Kyoto University | Method for promoting differentiation of pluripotent stem cells into cardiac muscle cells |
WO2012026491A1 (ja) | 2010-08-26 | 2012-03-01 | 国立大学法人京都大学 | 多能性幹細胞の心筋分化促進剤 |
CN103189508B (zh) | 2010-08-30 | 2016-08-10 | 生物载体株式会社 | 用于诱导多能性干细胞的组合物及其用途 |
EP3211070A1 (en) | 2010-08-31 | 2017-08-30 | Janssen Biotech, Inc. | Differentiation of human embryonic stem cells |
BR112013004616A2 (pt) | 2010-08-31 | 2016-07-05 | Janssen Biotech Inc | diferenciação das células tronco embrionárias humanas |
AU2011296383B2 (en) | 2010-08-31 | 2016-03-10 | Janssen Biotech, Inc. | Differentiation of pluripotent stem cells |
WO2012029994A1 (en) | 2010-09-02 | 2012-03-08 | Kyoto University | Pharmaceutical composition for prevention and treatment of amyotrophic lateral sclerosis |
JP5794588B2 (ja) | 2010-09-14 | 2015-10-14 | 国立大学法人京都大学 | 効率的な人工多能性幹細胞の樹立方法 |
US20130296183A1 (en) | 2010-09-17 | 2013-11-07 | President And Fellows Of Harvard College | Functional genomics assay for characterizing pluripotent stem cell utility and safety |
AU2011308496A1 (en) | 2010-10-01 | 2013-05-02 | Moderna Therapeutics, Inc. | Engineered nucleic acids and methods of use thereof |
CA2814713A1 (en) * | 2010-10-14 | 2012-04-19 | University Of Central Florida Research Foundation, Inc. | Cardiac induced pluripotent stem cells and methods of use in repair and regeneration of myocardium |
AU2011316830A1 (en) | 2010-10-22 | 2013-05-02 | Biotime Inc. | Methods of modifying transcriptional regulatory networks in stem cells |
WO2012057052A1 (ja) * | 2010-10-25 | 2012-05-03 | 公立大学法人横浜市立大学 | 幹細胞の安定的維持、複製を制御するためのペプチジルプロリルイソメラーゼPin1の利用 |
JP5875007B2 (ja) | 2010-11-02 | 2016-03-02 | 国立大学法人 熊本大学 | 腸細胞の製造方法 |
JP5888753B2 (ja) * | 2010-11-04 | 2016-03-22 | 国立大学法人京都大学 | 効率的な人工多能性幹細胞の樹立方法 |
US20130225443A1 (en) | 2010-11-05 | 2013-08-29 | Kyoto University | Method of examining polycystic kidney disease and method of screening for therapeutic agent of the disease |
JP5963309B2 (ja) * | 2010-11-09 | 2016-08-03 | 国立研究開発法人産業技術総合研究所 | 末梢血単球由来多能性幹細胞作製方法 |
EP2640829A4 (en) | 2010-11-17 | 2014-06-11 | Univ Kyoto | CARDIOMYOCYTE AND / OR CARDIOVORA CELL PROLIFERATING AGENTS AND METHOD FOR PROLIFERATING CARDIOMYOCYTES AND / OR CARDIOVORA CELLS |
JPWO2012074116A1 (ja) | 2010-12-02 | 2014-05-19 | 独立行政法人理化学研究所 | アロNKT細胞を用いた免疫療法およびそのためのT細胞抗原受容体(TCR)遺伝子のα鎖領域が均一なVα−Jαに再構成されている細胞および該細胞由来NKT細胞のバンキング |
US9506039B2 (en) | 2010-12-03 | 2016-11-29 | Kyoto University | Efficient method for establishing induced pluripotent stem cells |
JP5995237B2 (ja) | 2010-12-03 | 2016-09-21 | 国立大学法人京都大学 | 多能性幹細胞からの好酸球の製造方法 |
JP5888852B2 (ja) * | 2010-12-08 | 2016-03-22 | 学校法人近畿大学 | 免疫不全動物を用いた細胞の製法 |
KR102482184B1 (ko) | 2010-12-22 | 2022-12-28 | 페이트 세러퓨틱스, 인코포레이티드 | 단세포 분류 및 iPSC의 증강된 재프로그래밍을 위한 세포 배양 플랫폼 |
US9850466B2 (en) | 2011-01-19 | 2017-12-26 | The Regents Of The University Of California | Somatic cells with innate potential for pluripotency |
WO2012098260A1 (en) | 2011-01-21 | 2012-07-26 | Axiogenesis Ag | A non-viral system for the generation of induced pluripotent stem (ips) cells |
WO2012112458A2 (en) * | 2011-02-14 | 2012-08-23 | The Regents Of The University Of California | Compositions and methods for increasing reprogramming efficiency |
EP2678675B1 (en) | 2011-02-23 | 2017-10-11 | The Board of Trustees of the Leland Stanford Junior University | Methods of detecting aneuploidy in human embryos |
US9499789B2 (en) | 2011-02-23 | 2016-11-22 | Kyoto University | Method for producing dendritic cells from pluripotent stem cells |
GB201103600D0 (en) | 2011-03-01 | 2011-04-13 | Isis Innovation | Dendritic cells |
US9353370B2 (en) | 2011-03-30 | 2016-05-31 | Riken | Functional nucleic acid molecule and use thereof |
JP2014511687A (ja) | 2011-03-31 | 2014-05-19 | モデルナ セラピューティクス インコーポレイテッド | 工学操作された核酸の送達および製剤 |
JP6025067B2 (ja) | 2011-03-31 | 2016-11-16 | iHeart Japan株式会社 | 新規心筋細胞マーカー |
JP5892554B2 (ja) | 2011-03-31 | 2016-03-23 | 国立研究開発法人理化学研究所 | 未分化状態の制御剤およびその用途 |
JP5761826B2 (ja) | 2011-04-08 | 2015-08-12 | 国立大学法人大阪大学 | 改変ラミニンおよびその利用 |
PL2694642T3 (pl) | 2011-04-08 | 2019-01-31 | Institut National De La Santé Et De La Recherche Médicale (Inserm) | Sposób odmładzania komórek |
WO2012141181A1 (ja) * | 2011-04-11 | 2012-10-18 | 国立大学法人京都大学 | 核初期化物質 |
JP5759536B2 (ja) | 2011-04-20 | 2015-08-05 | 国立大学法人大阪大学 | 角膜上皮分化指向性iPS細胞 |
WO2012158561A1 (en) | 2011-05-13 | 2012-11-22 | The United States Of America As Represented By The Secretary, Dept. Of Health And Human Services | Use of zscan4 and zscan4-dependent genes for direct reprogramming of somatic cells |
WO2012168434A1 (en) | 2011-06-08 | 2012-12-13 | INSERM (Institut National de la Santé et de la Recherche Médicale) | Partial reprogramming of somatic cells to induced tissue stem (its) cells |
GB201110331D0 (en) | 2011-06-16 | 2011-08-03 | Isis Innovation | Method of cryopreserving pluripotent stem cells |
WO2013010045A1 (en) | 2011-07-12 | 2013-01-17 | Biotime Inc. | Novel methods and formulations for orthopedic cell therapy |
US20130029416A1 (en) | 2011-07-22 | 2013-01-31 | Tayaramma Thatava | Differentiating induced pluripotent stem cells into glucose-responsive, insulin-secreting progeny |
EP2734617B1 (en) | 2011-07-22 | 2017-04-12 | Centre National De La Recherche Scientifique | Use of cellular extracts for obtaining pluripotent stem cells |
EP3608423A1 (en) | 2011-07-25 | 2020-02-12 | Kyoto University | Method for screening induced pluripotent stem cells |
WO2013031826A1 (ja) * | 2011-08-29 | 2013-03-07 | 国立大学法人京都大学 | 核初期化物質 |
US9145547B2 (en) | 2011-08-30 | 2015-09-29 | Riken | Nuclear reprogrammed cells generated by introduction of a histone H2aa or TH2A gene, a histone H2ba or TH2B gene, or a phosphorylation-mimic of histone chaperon Npm2 gene, an Oct family gene and a klf family gene into a mammalian somatic cell |
US9464124B2 (en) | 2011-09-12 | 2016-10-11 | Moderna Therapeutics, Inc. | Engineered nucleic acids and methods of use thereof |
WO2013039087A1 (ja) | 2011-09-12 | 2013-03-21 | 国立大学法人 熊本大学 | 物質のスクリーニング方法 |
US9480695B2 (en) | 2011-09-29 | 2016-11-01 | The University Of Tokyo | Methods for inducing orexin neurons and agent for treating narcolepsy or eating disorder |
JP6120223B2 (ja) | 2011-09-29 | 2017-04-26 | 国立大学法人 東京大学 | オレキシンニューロンの誘導法 |
PT3682905T (pt) | 2011-10-03 | 2022-04-07 | Modernatx Inc | Nucleósidos, nucleótidos e ácidos nucleicos modificados e respetivas utilizações |
EP2770051B1 (en) | 2011-10-21 | 2017-09-27 | ARKRAY, Inc. | Method for culturing pluripotency-maintained singly dispersed cells by means of laminar flow |
GB2496375A (en) | 2011-10-28 | 2013-05-15 | Kymab Ltd | A non-human assay vertebrate comprising human antibody loci and human epitope knock-in, and uses thereof |
WO2013078433A1 (en) | 2011-11-23 | 2013-05-30 | University Of Hawaii | Auto-processing domains for polypeptide expression |
WO2013077423A1 (ja) | 2011-11-25 | 2013-05-30 | 国立大学法人京都大学 | 多能性幹細胞の培養方法 |
TW201335372A (zh) | 2011-11-30 | 2013-09-01 | Nat Cancer Ct | 誘導惡性幹細胞 |
GB201122047D0 (en) | 2011-12-21 | 2012-02-01 | Kymab Ltd | Transgenic animals |
AU2012347919B2 (en) | 2011-12-05 | 2017-02-02 | Factor Bioscience Inc. | Methods and products for transfecting cells |
US8497124B2 (en) | 2011-12-05 | 2013-07-30 | Factor Bioscience Inc. | Methods and products for reprogramming cells to a less differentiated state |
HRP20220717T1 (hr) | 2011-12-16 | 2022-07-22 | Modernatx, Inc. | Modificirani pripravci mrna |
US9890357B2 (en) | 2011-12-19 | 2018-02-13 | Kyoto University | Method for inducing differentiation of human pluripotent stem cells into intermediate mesoderm cells |
CA2860107C (en) | 2011-12-22 | 2021-06-01 | Janssen Biotech, Inc. | Differentiation of human embryonic stem cells into single hormonal insulin positive cells |
WO2013100080A1 (ja) | 2011-12-27 | 2013-07-04 | 国立大学法人大阪大学 | iPS細胞の腫瘍化を抑制することが可能な分化誘導方法 |
US20140377832A1 (en) | 2012-01-15 | 2014-12-25 | Yeda Research And Development Co. Ltd. | Induction of dedifferentiation of mesenchymal stromal cells |
JP6274510B2 (ja) | 2012-01-27 | 2018-02-07 | 国立大学法人京都大学 | 多能性幹細胞の心筋分化誘導法 |
CA2866590A1 (en) | 2012-03-07 | 2013-09-12 | Janssen Biotech, Inc. | Defined media for expansion and maintenance of pluripotent stem cells |
JP5920741B2 (ja) | 2012-03-15 | 2016-05-18 | iHeart Japan株式会社 | 人工多能性幹細胞から心筋および血管系混合細胞群を製造する方法 |
US20150064734A1 (en) | 2012-03-21 | 2015-03-05 | Kyoto University | Method for screening therapeutic and/or prophylactic agents for alzheimer's disease |
EP2828381A1 (en) | 2012-03-21 | 2015-01-28 | Rheinische Friedrich-Wilhelms-Universität Bonn | Induced neural stem cells |
US9254311B2 (en) | 2012-04-02 | 2016-02-09 | Moderna Therapeutics, Inc. | Modified polynucleotides for the production of proteins |
US9572897B2 (en) | 2012-04-02 | 2017-02-21 | Modernatx, Inc. | Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins |
EP2834358A4 (en) | 2012-04-02 | 2016-03-09 | Moderna Therapeutics Inc | MODIFIED POLYNUCLEOTIDES FOR THE PRODUCTION OF NUCLEAR PROTEINS |
US9283287B2 (en) | 2012-04-02 | 2016-03-15 | Moderna Therapeutics, Inc. | Modified polynucleotides for the production of nuclear proteins |
US9334475B2 (en) | 2012-04-06 | 2016-05-10 | Kyoto University | Method for inducing erythropoietin-producing cell |
WO2013158890A1 (en) * | 2012-04-19 | 2013-10-24 | Board Of Regents, The University Of Texas System | Generation of induced pluripotent stem cells by modulation of δnp63 or dcgr8 |
CA2874259C (en) | 2012-05-23 | 2021-02-09 | Kyoto University | Highly efficient method for establishing induced pluripotent stem cell |
RS64622B1 (sr) | 2012-05-25 | 2023-10-31 | Univ California | Metode i sastavi za modifikaciju ciljane dnk upravljenu pomoću rnk i za modulaciju transkripcije upravljanu rnk |
CA2875038A1 (en) | 2012-05-31 | 2013-12-05 | Auxogyn, Inc. | In vitro embryo blastocyst prediction methods |
US9884076B2 (en) | 2012-06-05 | 2018-02-06 | Capricor, Inc. | Optimized methods for generation of cardiac stem cells from cardiac tissue and their use in cardiac therapy |
CN108103006A (zh) | 2012-06-08 | 2018-06-01 | 詹森生物科技公司 | 人胚胎干细胞向胰腺内分泌细胞的分化 |
EP3838293A1 (en) | 2012-07-11 | 2021-06-23 | Tissuetech, Inc. | Compositions containing hc-ha/ptx3 complexes and methods of use thereof |
JP6373253B2 (ja) | 2012-07-17 | 2018-08-15 | 国立大学法人京都大学 | 新規心筋細胞マーカー |
CN113151179A (zh) | 2012-07-31 | 2021-07-23 | 阿格克斯治疗有限公司 | Hla g修饰的细胞及方法 |
JP6433896B2 (ja) | 2012-08-13 | 2018-12-05 | シーダーズ−サイナイ・メディカル・センターCedars−Sinai Medical Center | 組織再生のためのエキソソームおよびマイクロリボ核酸 |
WO2014057997A1 (ja) | 2012-10-09 | 2014-04-17 | Hayashi Nakanobu | 初期化ペプチド及びその用途 |
JP2014082956A (ja) | 2012-10-19 | 2014-05-12 | Somar Corp | 細胞培養基材、およびそれを用いた細胞培養方法並びに多能性幹細胞の分化誘導方法 |
JP6366582B2 (ja) * | 2012-10-23 | 2018-08-01 | 国立大学法人京都大学 | 効率的に人工多能性幹細胞を樹立する方法 |
US20150353889A1 (en) | 2012-10-30 | 2015-12-10 | Daiichi Sankyo Company, Limited | Mait-like Cells and Method for Manufacturing Same |
EP2914728B1 (en) | 2012-11-01 | 2020-07-08 | Factor Bioscience Inc. | Methods and products for expressing proteins in cells |
EP4074834A1 (en) | 2012-11-26 | 2022-10-19 | ModernaTX, Inc. | Terminally modified rna |
GB201222693D0 (en) * | 2012-12-17 | 2013-01-30 | Babraham Inst | Novel method |
WO2014103137A1 (ja) | 2012-12-27 | 2014-07-03 | ソニー株式会社 | 細胞分析システム、細胞分析プログラム及び細胞分析方法 |
WO2014104364A1 (ja) | 2012-12-28 | 2014-07-03 | 国立大学法人京都大学 | 人工多能性幹細胞、心筋細胞又はその前駆細胞の製造方法 |
SG10201709384SA (en) | 2012-12-31 | 2018-01-30 | Janssen Biotech Inc | Suspension and clustering of human pluripotent cells for differentiation into pancreatic endocrine cells |
US10370644B2 (en) | 2012-12-31 | 2019-08-06 | Janssen Biotech, Inc. | Method for making human pluripotent suspension cultures and cells derived therefrom |
SG10201707811XA (en) | 2012-12-31 | 2017-11-29 | Janssen Biotech Inc | Differentiation of human embryonic stem cells into pancreatic endocrine cells using hb9 regulators |
ES2837763T3 (es) | 2012-12-31 | 2021-07-01 | Janssen Biotech Inc | Cultivo de células madre embrionarias humanas en la interconexión aire-líquido para la diferenciación en células endocrinas pancreáticas |
US20160017441A1 (en) | 2013-01-16 | 2016-01-21 | Universal Bio Research Co., Ltd. | Method for identifying cells |
DK2951290T3 (en) | 2013-02-01 | 2018-01-22 | Us Health | PROCEDURE FOR PREPARING RETINAL PIGMENTAL EPIT (RPE) CELLS FROM INDUCED PLURIPOTENT STEM CELLS (IPSCS) |
CA2900741C (en) | 2013-02-01 | 2022-01-18 | Progyny, Inc. | Abnormal syngamy phenotypes observed with time lapse imaging for early identification of embryos with lower developmental potential |
US10450546B2 (en) | 2013-02-06 | 2019-10-22 | University Of Rochester | Induced pluripotent cell-derived oligodendrocyte progenitor cells for the treatment of myelin disorders |
JP6495658B2 (ja) | 2013-02-08 | 2019-04-03 | 国立大学法人京都大学 | 巨核球及び血小板の製造方法 |
JP6494903B2 (ja) | 2013-02-14 | 2019-04-03 | ソニー株式会社 | 分析システム、分析プログラム及び分析方法 |
WO2014136581A1 (ja) | 2013-03-06 | 2014-09-12 | 国立大学法人京都大学 | 多能性幹細胞の培養システム及び多能性幹細胞の継代方法 |
EP2966166B1 (en) | 2013-03-08 | 2019-04-03 | Kyoto University | Promoter of differentiation of pluripotent stem cell into myocardium, which comprises egf receptor inhibitor |
WO2014153230A1 (en) | 2013-03-14 | 2014-09-25 | The Regents Of The University Of California | In vitro production of medial ganglionic eminence precursor cells |
US8980864B2 (en) | 2013-03-15 | 2015-03-17 | Moderna Therapeutics, Inc. | Compositions and methods of altering cholesterol levels |
US10519421B2 (en) | 2013-03-21 | 2019-12-31 | Kyoto University | Induction of motor neurons from pluripotent stem cells |
JP6473686B2 (ja) | 2013-03-25 | 2019-02-20 | 公益財団法人神戸医療産業都市推進機構 | 細胞の選別方法 |
GB201306589D0 (en) | 2013-04-11 | 2013-05-29 | Abeterno Ltd | Live cell imaging |
US10961508B2 (en) | 2013-04-12 | 2021-03-30 | Kyoto University | Method for inducing alveolar epithelial progenitor cells |
US9822342B2 (en) | 2013-05-14 | 2017-11-21 | Kyoto University | Method of efficiently inducing cardiomyocytes |
CN105247041B (zh) | 2013-05-31 | 2018-04-20 | 爱心细胞有限公司 | 组入有水凝胶的层叠细胞膜片 |
US11085067B2 (en) | 2013-06-10 | 2021-08-10 | President And Fellows Of Harvard College | Early developmental genomic assay for characterizing pluripotent stem cell utility and safety |
KR102145967B1 (ko) | 2013-06-11 | 2020-08-19 | 고쿠리츠 다이가쿠 호진 교토 다이가쿠 | 신장 전구세포의 제조 방법 및 신장 전구세포를 포함하는 의약 |
WO2014200030A1 (ja) | 2013-06-12 | 2014-12-18 | 国立大学法人京都大学 | 人工多能性幹細胞の選別方法および血球への分化誘導方法 |
EP3031905A4 (en) | 2013-08-07 | 2017-04-26 | Kyoto University | Method for producing pancreatic hormone-producing cell |
JP6667897B2 (ja) | 2013-08-23 | 2020-03-18 | 国立研究開発法人理化学研究所 | 蛍光特性を示すポリペプチド、およびその利用 |
EP3040416B1 (en) | 2013-08-28 | 2019-05-01 | Gifu University | Method for producing induced pluripotent stem cells |
WO2015030149A1 (ja) | 2013-08-29 | 2015-03-05 | 国立大学法人鳥取大学 | 細胞のアンチエイジングに関連する生体分子群 |
WO2015034710A2 (en) | 2013-09-05 | 2015-03-12 | Tempo Bioscience Inc. | Human cellular models with biosensors |
SG11201601720RA (en) | 2013-09-05 | 2016-04-28 | Univ Kyoto | New method for inducing dopamine-producing neural precursor cells |
WO2015037535A1 (ja) | 2013-09-12 | 2015-03-19 | 株式会社カネカ | 人工多能性幹細胞の分化誘導方法及び選別方法 |
JP6333830B2 (ja) | 2013-09-13 | 2018-05-30 | 国立大学法人京都大学 | 多能性幹細胞の心筋分化を促進する化合物 |
US10975358B2 (en) | 2013-09-24 | 2021-04-13 | Id Pharma Co., Ltd. | Method for improving efficiency of inducing pluripotent stem cell |
EP3052106A4 (en) | 2013-09-30 | 2017-07-19 | ModernaTX, Inc. | Polynucleotides encoding immune modulating polypeptides |
EP3052521A1 (en) | 2013-10-03 | 2016-08-10 | Moderna Therapeutics, Inc. | Polynucleotides encoding low density lipoprotein receptor |
US20160304840A1 (en) | 2013-11-01 | 2016-10-20 | New England Biolabs, Inc. | Method for Producing Induced Pluripotent Stem Cells |
JP6635505B2 (ja) | 2013-11-01 | 2020-01-29 | 国立大学法人京都大学 | 新規軟骨細胞誘導方法 |
JP6942925B2 (ja) | 2013-11-08 | 2021-09-29 | ソニーグループ株式会社 | 細胞分析システム、細胞分析プログラム及び細胞分析方法 |
US11767507B2 (en) | 2013-11-08 | 2023-09-26 | The Mclean Hospital Corporation | Methods for efficient generation of GABAergic interneurons from pluripotent stem cells |
CN104630136B (zh) * | 2013-11-15 | 2019-10-01 | 中国科学院广州生物医药与健康研究院 | 一种制备诱导多能性干细胞的方法以及该方法中所使用的组合物及其应用 |
US9932607B2 (en) | 2013-11-15 | 2018-04-03 | The Board Of Trustees Of The Leland Stanford Junior University | Site-specific integration of transgenes into human cells |
AU2014350051A1 (en) | 2013-11-18 | 2016-07-07 | Crispr Therapeutics Ag | CRISPR-Cas system materials and methods |
JP6536871B2 (ja) | 2013-12-02 | 2019-07-03 | 国立大学法人京都大学 | Fgfr3病の予防および治療剤ならびにそのスクリーニング方法 |
KR102070967B1 (ko) * | 2013-12-10 | 2020-01-29 | 한국한의학연구원 | 사군자탕을 유효성분으로 포함하는, 세포의 유도만능줄기세포로의 리프로그래밍 촉진용 조성물 및 이를 이용한 유도만능줄기세포의 제조방법 |
WO2015087231A1 (en) | 2013-12-11 | 2015-06-18 | Pfizer Limited | Method for producing retinal pigment epithelial cells |
EP3080266B1 (en) | 2013-12-12 | 2021-02-03 | The Regents of The University of California | Methods and compositions for modifying a single stranded target nucleic acid |
US10100284B2 (en) | 2013-12-25 | 2018-10-16 | Toagosei Co. Ltd. | Method for inducing differentiation of pluripotent stem cells into endodermal cells |
EP2896688A1 (en) | 2014-01-20 | 2015-07-22 | Centre National de la Recherche Scientifique (CNRS) | A method of producing beta pancreatic cells from progenitor cells through the use of hydrogen peroxide |
US9770489B2 (en) | 2014-01-31 | 2017-09-26 | Factor Bioscience Inc. | Methods and products for nucleic acid production and delivery |
EP3604499A1 (en) | 2014-03-04 | 2020-02-05 | Fate Therapeutics, Inc. | Improved reprogramming methods and cell culture platforms |
US11066649B2 (en) | 2014-03-19 | 2021-07-20 | Institut National De La Sante Et De La Recherche Medicale (Inserm) | Method for inducing human cholangiocyte differentiation |
ES2837840T3 (es) | 2014-03-20 | 2021-07-01 | Ares Trading Sa | Medida cuantitativa de la cinética de desarrollo de la morfología de mórula y blastocisto humanos |
US10538740B2 (en) | 2014-03-20 | 2020-01-21 | Kyoto University | Method for sorting cardiomyocytes |
EP3128003B1 (en) | 2014-03-31 | 2023-11-01 | Ajinomoto Co., Inc. | Medium for stem cell use |
SG10201810739VA (en) | 2014-05-16 | 2019-01-30 | Janssen Biotech Inc | Use of small molecules to enhance mafa expression in pancreatic endocrine cells |
CN106536718B (zh) | 2014-05-21 | 2021-04-27 | 国立大学法人京都大学 | 胰芽细胞的制造方法及含有胰芽细胞的胰疾病治疗剂 |
EP3150705B1 (en) | 2014-05-30 | 2019-05-15 | Kyoto University | Method for inducing myocardial differentiation of pluripotent stem cells using low-molecular compound |
WO2015195769A2 (en) | 2014-06-18 | 2015-12-23 | President And Fellows Of Harvard College | Optogenetic probes for measuring membrane potential |
US10138469B2 (en) | 2014-06-23 | 2018-11-27 | Toagosei Co., Ltd. | Synthetic peptide and use thereof |
EP3929302A1 (en) | 2014-07-14 | 2021-12-29 | Chugai Seiyaku Kabushiki Kaisha | Method for identifying epitope on protein |
US10570418B2 (en) | 2014-09-02 | 2020-02-25 | The Regents Of The University Of California | Methods and compositions for RNA-directed target DNA modification |
US11357799B2 (en) | 2014-10-03 | 2022-06-14 | Cedars-Sinai Medical Center | Cardiosphere-derived cells and exosomes secreted by such cells in the treatment of muscular dystrophy |
JP6598185B2 (ja) | 2014-11-07 | 2019-10-30 | 国立大学法人京都大学 | 軟骨過形成疾患の予防および治療剤ならびにそのスクリーニング方法 |
WO2016085765A1 (en) | 2014-11-25 | 2016-06-02 | President And Fellows Of Harvard College | Methods for generation of podocytes from pluripotent stem cells and cells produced by the same |
SG11201704690TA (en) | 2014-12-17 | 2017-07-28 | Fundación Para La Investigación Mèdica Aplicada | Nucleic acid constructs and gene therapy vectors for use in the treatment of wilson disease |
PT3233129T (pt) | 2014-12-17 | 2020-04-15 | Fundacion Para La Investig Medica Aplicada | Construções de ácido nucleico e vetores de terapia génica para utilização no tratamento de doença de wilson e outras condições |
US10711249B2 (en) | 2014-12-26 | 2020-07-14 | Kyoto University | Method for inducing hepatocytes |
WO2016115333A1 (en) | 2015-01-15 | 2016-07-21 | President And Fellows Of Harvard College | Optical selection of cells |
WO2016114405A1 (ja) | 2015-01-16 | 2016-07-21 | 国立研究開発法人産業技術総合研究所 | ステルス性を有するrnaを使った遺伝子発現系および当該rnaを含む遺伝子導入・発現ベクター |
EP3543339A1 (en) | 2015-02-13 | 2019-09-25 | Factor Bioscience Inc. | Nucleic acid products and methods of administration thereof |
JP2018510649A (ja) | 2015-02-17 | 2018-04-19 | ユニバーシティー ヘルス ネットワーク | 洞房結節様ペースメーカー心筋細胞および心室様心筋細胞を作製および使用するための方法 |
PT3059307T (pt) | 2015-02-20 | 2019-01-23 | Inst Nat Sante Rech Med | Utilização de uma laminina para a diferenciação de células pluripotentes em células de linha hepatocitária |
US11299712B2 (en) | 2015-03-06 | 2022-04-12 | Kyoto University | Method for inducing differentiation of alveolar epithelial cells |
WO2016148253A1 (ja) | 2015-03-18 | 2016-09-22 | 小野薬品工業株式会社 | ナイーブ型多能性幹細胞の製造方法 |
WO2016165788A1 (en) | 2015-04-14 | 2016-10-20 | Uab Ferentis | Collagen mimetic peptide |
WO2016167329A1 (ja) * | 2015-04-14 | 2016-10-20 | 国立大学法人京都大学 | 体細胞への分化誘導に適した幹細胞クローンを製造する方法 |
EP3081638A1 (en) | 2015-04-16 | 2016-10-19 | Kyoto University | Method for producing pseudo-islets |
US11359180B2 (en) | 2015-04-28 | 2022-06-14 | Toagosei Co., Ltd. | Method for producing myocardial cells using synthetic peptide |
US9724432B2 (en) | 2015-04-30 | 2017-08-08 | University Of Rochester | Non-human mammal model of human degenerative disorder, uses thereof, and method of treating human degenerative disorder |
FR3037338B1 (fr) | 2015-06-12 | 2020-02-28 | Philippe Nirde | Procede de greffe de cellule cardiaque sur la membrane choriallantoide d'œuf feconde |
WO2017002300A1 (en) | 2015-06-30 | 2017-01-05 | Sony Corporation | Information processing apparatus, information processing system, and information processing method |
JP6746945B2 (ja) | 2015-06-30 | 2020-08-26 | ソニー株式会社 | 情報処理装置、情報処理システム及び情報処理方法 |
CN107709553A (zh) * | 2015-07-10 | 2018-02-16 | 心脏康复株式会社 | 高品质iPS细胞的制造方法 |
US10669529B2 (en) | 2015-07-17 | 2020-06-02 | Kyoto University | Method for inducing vascular endothelial cells |
DK3344758T3 (da) | 2015-09-01 | 2021-11-01 | Ncardia B V | Fremgangsmåde in vitro til differentiering af en human pluripotent stamcellepopulation i en kardiomyocytcellepopulation |
DK3347457T3 (da) | 2015-09-08 | 2022-01-17 | Fujifilm Cellular Dynamics Inc | MACS-baseret oprensning af stamcelleafledt retinalt pigmentepitel |
KR20180042437A (ko) | 2015-09-08 | 2018-04-25 | 더 유나이티드 스테이츠 오브 어메리카, 애즈 리프리젠티드 바이 더 세크러테리, 디파트먼트 오브 헬쓰 앤드 휴먼 서비씨즈 | 임상 등급 망막 색소 상피 세포의 재현성 있는 분화 방법 |
IL257974B (en) | 2015-09-11 | 2022-07-01 | Astellas Pharma Inc | A method for producing kidney stem cells |
CA2998287A1 (en) | 2015-09-24 | 2017-04-20 | Crispr Therapeutics Ag | Novel family of rna-programmable endonucleases and their uses in genome editing and other applications |
JP6691756B2 (ja) | 2015-09-29 | 2020-05-13 | 東亞合成株式会社 | 合成ペプチドを用いた神経幹細胞の生産方法 |
WO2017059241A1 (en) | 2015-10-02 | 2017-04-06 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Lentiviral protein delivery system for rna-guided genome editing |
KR20180055901A (ko) | 2015-10-05 | 2018-05-25 | 오리그3엔, 인코포레이티드 | 간 기능장애의 확인 및 개선에 기반한 파킨슨병의 진단 및 치료 |
AU2016338680B2 (en) | 2015-10-16 | 2022-11-17 | Fate Therapeutics, Inc. | Platform for the induction and maintenance of ground state pluripotency |
US10947502B2 (en) | 2015-10-20 | 2021-03-16 | FUJIFILM Cellular Dynamics, Inc. | Methods for directed differentiation of pluripotent stem cells to immune cells |
JP2016011317A (ja) * | 2015-10-21 | 2016-01-21 | 加治佐 功 | ゲノム編集用クリスパーキャス9による老化遺伝子切り取り若返り経口不老不死薬7 |
SG11201804197RA (en) | 2015-11-18 | 2018-06-28 | Orbis Health Solutions Llc | T7 alpha viral vector system |
WO2017123662A1 (en) | 2016-01-11 | 2017-07-20 | Cedars-Sinai Medical Center | Cardiosphere-derived cells and exosomes secreted by such cells in the treatment of heart failure with preserved ejection fraction |
GB201601503D0 (en) | 2016-01-27 | 2016-03-09 | Isis Innovation | Dendritic cells |
EP3417061B1 (en) | 2016-02-18 | 2022-10-26 | The Regents of the University of California | Methods and compositions for gene editing in stem cells |
CA3017871A1 (en) | 2016-03-18 | 2017-09-21 | Kyoto University | Method for freezing aggregates of pluripotent stem cell-derived cardiomyocytes |
WO2017164746A1 (en) | 2016-03-25 | 2017-09-28 | Pluriomics B.V. | In vivo method for differentiating human pluripotent stem cells into atrial cardiomyocytes |
MA45479A (fr) | 2016-04-14 | 2019-02-20 | Janssen Biotech Inc | Différenciation de cellules souches pluripotentes en cellules de l'endoderme de l'intestin moyen |
HUE056387T2 (hu) | 2016-04-15 | 2022-02-28 | Univ Kyoto | Eljárás antigénspecifikus CD8-pozitív T-sejtek indukálására |
KR102312123B1 (ko) | 2016-04-22 | 2021-10-13 | 고쿠리츠 다이가쿠 호진 교토 다이가쿠 | 도파민 생산 신경전구세포의 제조방법 |
WO2017210652A1 (en) | 2016-06-03 | 2017-12-07 | Cedars-Sinai Medical Center | Cdc-derived exosomes for treatment of ventricular tachyarrythmias |
WO2017216771A2 (en) | 2016-06-17 | 2017-12-21 | Genesis Technologies Limited | Crispr-cas system, materials and methods |
AU2017290805B2 (en) | 2016-07-01 | 2023-11-16 | Research Development Foundation | Elimination of proliferating cells from stem cell-derived grafts |
US11866733B2 (en) | 2016-08-01 | 2024-01-09 | University of Pittsburgh—of the Commonwealth System of Higher Education | Human induced pluripotent stem cells for high efficiency genetic engineering |
US10354218B2 (en) | 2016-08-04 | 2019-07-16 | Fanuc Corporation | System and method for iPS cell bank using internet technology |
US10373109B2 (en) | 2016-08-04 | 2019-08-06 | Fanuc Corporation | System and method for iPS cell bank using media |
JP2019528284A (ja) | 2016-08-17 | 2019-10-10 | ファクター バイオサイエンス インコーポレイテッド | 核酸産物およびその投与方法 |
EP3508569B1 (en) | 2016-09-02 | 2023-04-19 | Takara Bio Inc. | Method for obtaining microglia from pluripotent stem cells |
EP3515459A4 (en) | 2016-09-20 | 2020-08-05 | Cedars-Sinai Medical Center | CELLS DERIVED FROM CARDIOSPHERES AND THEIR EXTRACELLULAR VESICLES TO DELAY OR REVERSE AGING AND AGE-RELATED DISORDERS |
AU2017340634B2 (en) | 2016-10-05 | 2022-06-02 | FUJIFILM Cellular Dynamics, Inc. | Generating mature lineages from induced pluripotent stem cells with MECP2 disruption |
WO2018069927A1 (en) | 2016-10-10 | 2018-04-19 | The National Institute for Biotechnology in the Negev Ltd. | Non-cytotoxic modified cells and use thereof |
US12063913B2 (en) | 2016-10-31 | 2024-08-20 | National University Corporation Tottori University | Human antibody-producing non-human animal and method for preparing human antibodies using same |
JP7222900B2 (ja) | 2016-11-09 | 2023-02-15 | ザ ユナイテッド ステイツ オブ アメリカ アズ リプリゼンテッド バイ ザ セクレタリー、デパートメント オブ ヘルス アンド ヒューマン サービシーズ | 細胞療法および創薬のための3d血管化ヒト眼組織 |
US20200141921A1 (en) | 2016-12-27 | 2020-05-07 | Sumitomo Chemical Company, Limited | Evaluation method and selection method for induced pluripotent stem cells, and production method for induced pluripotent stem cells |
US12006512B2 (en) | 2017-01-20 | 2024-06-11 | Kyoto University | Method for producing CD8alpha+beta+ cytotoxic t cells |
US20210130785A1 (en) | 2017-01-26 | 2021-05-06 | Osaka University | Medium for inducing differentiation of stem cells into mesodermal cells and method for producing mesodermal cells |
CN110234754B (zh) | 2017-01-27 | 2024-02-20 | 株式会社钟化 | 内胚层细胞团、和由多能细胞制造三胚层中的任意胚层的细胞团的方法 |
JP7131775B2 (ja) | 2017-02-06 | 2022-09-06 | 国立研究開発法人国立がん研究センター | 新規t細胞受容体 |
US11530388B2 (en) | 2017-02-14 | 2022-12-20 | University of Pittsburgh—of the Commonwealth System of Higher Education | Methods of engineering human induced pluripotent stem cells to produce liver tissue |
US10828330B2 (en) | 2017-02-22 | 2020-11-10 | IO Bioscience, Inc. | Nucleic acid constructs comprising gene editing multi-sites and uses thereof |
WO2018159805A1 (ja) | 2017-03-03 | 2018-09-07 | 国立大学法人京都大学 | 膵前駆細胞の製造方法 |
EP3597734A4 (en) | 2017-03-14 | 2021-03-03 | Kyoto University | PROCESS FOR THE PRODUCTION OF AUXILIARY T LYMPHOCYTES FROM PLURIPOTENT STEM CELLS |
KR102641031B1 (ko) | 2017-03-28 | 2024-02-28 | 아지노모토 가부시키가이샤 | 미분화 유지 배지 첨가제 |
EA201992469A1 (ru) | 2017-04-18 | 2020-05-27 | Фуджифилм Селльюлар Дайнамикс, Инк. | Антигенспецифические иммунные эффекторные клетки |
CA3059910A1 (en) | 2017-04-19 | 2018-10-25 | Cedars-Sinai Medical Center | Methods and compositions for treating skeletal muscular dystrophy |
WO2018199662A1 (ko) * | 2017-04-26 | 2018-11-01 | 주식회사 툴젠 | sRAGE를 분비하는 줄기세포를 포함하는 알츠하이머병의 예방 또는 치료용 약학 조성물 |
US11690876B2 (en) | 2017-05-10 | 2023-07-04 | University Of Rochester | Methods of treating neuropsychiatric disorders |
CN117802033A (zh) | 2017-05-25 | 2024-04-02 | 国立大学法人京都大学 | 由多能干细胞制造中胚层谱系原条细胞的方法 |
EP3406712A1 (en) | 2017-05-26 | 2018-11-28 | Fundación Centro Nacional De Investigaciones Oncológicas Carlos III | Method for expanding stemness and differentiation potential of pluripotent cells |
EP3640318A4 (en) | 2017-06-14 | 2021-03-17 | Takeda Pharmaceutical Company Limited | CELL SEALING DEVICE |
EP3643780A4 (en) | 2017-06-19 | 2021-04-07 | Foundation for Biomedical Research and Innovation at Kobe | METHOD FOR PREDICTING THE DIFFERENTIATION CAPACITY OF PLURIPOTENT STEM CELLS, AND ASSOCIATED REAGENT |
JP6758631B2 (ja) | 2017-06-19 | 2020-09-23 | 国立大学法人大阪大学 | 角膜内皮細胞マーカー及びその利用 |
US10660523B2 (en) | 2017-07-07 | 2020-05-26 | Hideo Ando | Light-source unit, measurement apparatus, near-infrared microscopic apparatus, optical detection method, imaging method, calculation method, functional bio-related substance, state management method, and manufacturing method |
US20210363496A1 (en) | 2017-10-17 | 2021-11-25 | Kyoto University | Method for obtaining artificial neuromuscular junction from pluripotent stem cells |
US20200332315A1 (en) | 2017-11-02 | 2020-10-22 | National University Corporation Tottori University | Method for high production of protein using mammalian artificial chromosome vector |
WO2019092507A2 (en) | 2017-11-09 | 2019-05-16 | Crispr Therapeutics Ag | Crispr/cas systems for treatment of dmd |
CN111630155B (zh) | 2017-11-15 | 2024-05-03 | 沃泰克斯药物股份有限公司 | 胰岛细胞制备性组合物和使用方法 |
BR112020010539A2 (pt) | 2017-11-30 | 2020-11-17 | Kyoto University | métodos para produção e para proliferação de células da crista neural, para produção de células nervosas, células da glia, células estromais mesenquimais, células ósseas, condrócitos, células da córnea ou células pigmentares e para cultivar células da crista neural, meio, estoque congelado, e, uso de um meio |
MA50942A (fr) | 2017-12-01 | 2020-10-07 | Encoded Therapeutics Inc | Protéines de liaison à l'adn modifiées |
CN111801417A (zh) | 2017-12-14 | 2020-10-20 | 克里斯珀医疗股份公司 | 新的rna-可编程的内切核酸酶系统及其在基因组编辑和其他应用中的用途 |
EP3727351A4 (en) | 2017-12-20 | 2021-10-06 | Cedars-Sinai Medical Center | MODIFIED EXTRACELLULAR VESICLES FOR IMPROVED TISSUE DELIVERY |
TWI821230B (zh) | 2017-12-22 | 2023-11-11 | 日商千紙鶴治療公司 | 細胞培養裝置、培養液吸引器及細胞培養方法 |
CN112352054A (zh) | 2018-03-16 | 2021-02-09 | 国立大学法人鸟取大学 | 小鼠人工染色体载体及其用途 |
WO2019182157A1 (ja) | 2018-03-19 | 2019-09-26 | 国立大学法人京都大学 | ハイドロゲルカプセル |
CA3092497A1 (en) | 2018-03-19 | 2019-09-26 | Crispr Therapeutics Ag | Novel rna-programmable endonuclease systems and uses thereof |
US20210010030A1 (en) | 2018-03-22 | 2021-01-14 | Nserm (Institut National De La Santé Et De La Recherche Médicale) | Method for reprogramming somatic cells |
BR112020019579A8 (pt) | 2018-03-30 | 2022-10-18 | Takeda Pharmaceuticals Co | Composto, promotor de maturação de cardiomiócito, método para preparação de um cardiomiócito maduro, e, cardiomiócito maduro |
JP7440868B2 (ja) | 2018-03-30 | 2024-02-29 | 国立大学法人京都大学 | 細胞の製造方法 |
JPWO2019189758A1 (ja) | 2018-03-30 | 2021-04-01 | 味の素株式会社 | ポリリジン類縁体を含む、細胞増殖促進用組成物 |
JP7344486B2 (ja) | 2018-03-30 | 2023-09-14 | 国立大学法人京都大学 | 心筋細胞成熟促進剤 |
US11268070B2 (en) | 2018-04-16 | 2022-03-08 | Cellular Engineering Technologies, Inc. | Methods for creating integration-free, virus-free, exogenous oncogene-free IPS cells and compositions for use in such methods |
US12110503B2 (en) | 2018-04-20 | 2024-10-08 | FUJIFILM Cellular Dynamics, Inc. | Method for differentiation of ocular cells and use thereof |
EP3812456A4 (en) | 2018-04-23 | 2022-01-12 | Kyoto University | GROWTH INHIBITOR |
JP7311116B2 (ja) | 2018-04-27 | 2023-07-19 | 株式会社カネカ | 膵臓β細胞の製造方法 |
US20210254006A1 (en) | 2018-06-06 | 2021-08-19 | Ideaya Biosciences, Inc. | Methods of culturing and/or expanding stem cells and/or lineage committed progenitor cells using lactam compounds |
CA3103663A1 (en) | 2018-06-18 | 2019-12-26 | University Of Rochester | Inhibition of re1-silencing transcription factor in the treatment of schizophrenia and other neuropsychiatric disorders |
CN112654366A (zh) | 2018-06-21 | 2021-04-13 | 罗切斯特大学 | 治疗或抑制亨廷顿病发作的方法 |
US20210130777A1 (en) | 2018-07-13 | 2021-05-06 | Kyoto University | Method for producing gamma delta t cells |
US20210299331A1 (en) | 2018-07-19 | 2021-09-30 | Kyoto University | Pluripotent stem cell-derived plate-shaped cartilage and method for producing the same |
EP3828262A4 (en) | 2018-07-23 | 2022-03-30 | Kyoto University | NOVEL RENAL PROGENITOR CELL MARKER AND METHOD OF RENAL PROGENITOR CELL CONCENTRATION USING THE SAME |
CN112912490B (zh) | 2018-08-03 | 2023-12-26 | 千纸鹤治疗公司 | 细胞制造方法 |
CA3108275A1 (en) | 2018-08-10 | 2020-02-13 | Vertex Pharmaceuticals Incorporated | Stem cell derived islet differentiation |
US20220145329A1 (en) | 2018-08-10 | 2022-05-12 | Kyoto University | Method for transfection into cardiomyocytes using cationic lipid |
TW202016293A (zh) | 2018-08-10 | 2020-05-01 | 國立大學法人京都大學 | Cd3陽性細胞的製造方法 |
EP3838279A4 (en) | 2018-08-14 | 2022-04-27 | National Center for Global Health and Medicine | BROWN ADIPOCYTE SUPERNATAN, METHOD FOR PREPARATION, AND USE |
EA202190582A1 (ru) | 2018-08-22 | 2021-05-27 | Киото Юниверсити | Способ получения энтеральных нейральных клеток-предшественников |
EA202190624A1 (ru) | 2018-08-31 | 2021-06-09 | Нойл-Иммьюн Байотек, Инк. | Car-экспрессирующие т-клетки и car-экспрессирующий вектор |
SG11202102748WA (en) | 2018-09-19 | 2021-04-29 | Takeda Pharmaceuticals Co | Insulin-producing cells |
WO2020075823A1 (ja) | 2018-10-10 | 2020-04-16 | 国立大学法人鳥取大学 | 微小核細胞融合法による目的dnaを含む動物細胞の作製方法 |
EP3800246A4 (en) | 2018-10-10 | 2022-04-20 | National University Corporation Tottori University | METHOD FOR THE PRODUCTION OF HUMAN INDUCED PLURIPOTENT STEM CELLS CONTAINING AN EXOGENOUS CHROMOSOME |
ES2948378T3 (es) | 2018-10-12 | 2023-09-11 | Vivet Therapeutics | Transgén con codones optimizados para el tratamiento de la colestasis intrahepática familiar progresiva de tipo 3 (PFIC3) |
EP3868869A4 (en) | 2018-10-15 | 2022-08-03 | Public University Corporation Yokohama City University | NUTRITIONAL COMPOSITION |
EP3875578A4 (en) | 2018-10-31 | 2022-08-10 | Kyoto University | METHOD FOR GENERATING PLURIPOTENT STEM CELLS WITH RELEASED DIFFERENTIATION RESISTANCE TO MESENDODERM |
CN112955186A (zh) | 2018-11-07 | 2021-06-11 | 维韦特治疗公司 | 用于治疗进行性家族性肝内胆汁淤积症2型(pfic2)的密码子优化的abcb11转基因 |
CA3118936A1 (en) | 2018-11-16 | 2020-05-22 | Encoded Therapeutics, Inc. | Compositions and methods for treating wilson's disease |
CN117982733A (zh) | 2018-11-19 | 2024-05-07 | 美国政府(由卫生和人类服务部的部长所代表) | 可生物降解的组织置换植入物及其用途 |
AU2019386140A1 (en) | 2018-11-28 | 2021-06-24 | Board Of Regents, The University Of Texas System | Multiplex genome editing of immune cells to enhance functionality and resistance to suppressive environment |
CN113195710A (zh) | 2018-12-06 | 2021-07-30 | 麒麟控股株式会社 | T细胞或nk细胞的制造方法、t细胞或nk细胞的培养用培养基、t细胞或nk细胞的培养方法、维持未分化t细胞的未分化状态的方法和t细胞或nk细胞的增殖促进剂 |
AU2019396450A1 (en) | 2018-12-11 | 2021-06-24 | University Of Rochester | Methods of treating schizophrenia and other neuropsychiatric disorders |
WO2020130147A1 (ja) | 2018-12-21 | 2020-06-25 | 国立大学法人京都大学 | ルブリシン局在軟骨様組織、その製造方法及びそれを含む関節軟骨損傷治療用組成物 |
CN113226475A (zh) | 2018-12-26 | 2021-08-06 | 麒麟控股株式会社 | 改造tcr及其制造方法 |
TW202039543A (zh) | 2018-12-27 | 2020-11-01 | 國立大學法人京都大學 | T細胞受體的變體 |
CN113382768A (zh) | 2019-02-01 | 2021-09-10 | 国立大学法人京都大学 | 细胞的检测方法 |
US20230057355A1 (en) | 2019-02-13 | 2023-02-23 | University Of Rochester | Gene networks that mediate remyelination of the human brain |
WO2020175592A1 (ja) | 2019-02-26 | 2020-09-03 | 国立大学法人東北大学 | iPS細胞を用いた骨芽細胞塊の作製法 |
WO2020181101A1 (en) | 2019-03-07 | 2020-09-10 | The Regents Of The University Of California | Crispr-cas effector polypeptides and methods of use thereof |
WO2020209959A1 (en) | 2019-03-08 | 2020-10-15 | Crispr Therapeutics Ag | Nucleobase-editing fusion protein systems, compositions, and uses thereof |
US20220145274A1 (en) | 2019-03-12 | 2022-05-12 | Crispr Therapeutics Ag | Novel high fidelity rna-programmable endonuclease systems and uses thereof |
EP3950933A4 (en) | 2019-03-29 | 2023-01-11 | Kaneka Corporation | CELL POPULATION COMPRISING PLURIPOTENT STEM CELLS AND METHOD FOR PRODUCING IT |
US20220252575A1 (en) | 2019-03-29 | 2022-08-11 | Public University Corporation Yokohama City University | Screening method and toxicity evaluation method |
CN113993528A (zh) | 2019-04-10 | 2022-01-28 | 千纸鹤治疗公司 | 类生体组织结构体的制造方法 |
US20220235331A1 (en) | 2019-04-17 | 2022-07-28 | Keio University | Production method and kit of induced pluripotent stem cells |
EP3966327A1 (en) | 2019-05-08 | 2022-03-16 | Vertex Pharmaceuticals Incorporated | Crispr/cas all-in-two vector systems for treatment of dmd |
CA3140384A1 (en) | 2019-05-15 | 2020-11-19 | Ajinomoto Co., Inc. | Method for purifying neural crest cells or corneal epithelial cells |
JPWO2020235319A1 (ja) | 2019-05-20 | 2020-11-26 | ||
EP3985104A4 (en) | 2019-06-11 | 2023-04-12 | Kyoto University | PROCEDURE FOR GENERATING A KIDNEY INTERSTITIAL CELL |
WO2020264072A1 (en) | 2019-06-25 | 2020-12-30 | Semma Therapeutics, Inc. | Enhanced differentiation of beta cells |
CN114450418A (zh) | 2019-07-19 | 2022-05-06 | 东京毅力科创株式会社 | 细胞的分化状态的评价方法 |
US10501404B1 (en) | 2019-07-30 | 2019-12-10 | Factor Bioscience Inc. | Cationic lipids and transfection methods |
WO2021030424A1 (en) | 2019-08-13 | 2021-02-18 | Semma Therapeutics, Inc. | Pancreatic differentiation |
CA3151819A1 (en) | 2019-08-20 | 2021-02-25 | Orizuru Therapeutics, Inc. | Method for enriching cardiac myocytes |
WO2021066076A1 (ja) | 2019-10-01 | 2021-04-08 | 国立大学法人京都大学 | 尿管芽先端部細胞の単離方法 |
CN114929854A (zh) | 2019-10-21 | 2022-08-19 | 千纸鹤治疗公司 | 增殖抑制剂 |
CN114729318A (zh) | 2019-11-01 | 2022-07-08 | 国立大学法人京都大学 | T细胞的制备方法 |
JPWO2021095811A1 (ja) | 2019-11-12 | 2021-05-20 | ||
JPWO2021106832A1 (ja) | 2019-11-25 | 2021-06-03 | ||
EP4074321A4 (en) | 2019-12-12 | 2024-01-03 | National University Corporation Chiba University | FREEZE DRIED PREPARATION CONTAINING MEGAKARYOCYTES AND PLATELETS |
FR3105260A1 (fr) | 2019-12-20 | 2021-06-25 | Centre National De La Recherche Scientifique (Cnrs) | Modèle organoïde cardiaque vascularisé apres incorporation de cardiomyocytes dérivés de cellules souches pluripotentes induites humaines |
MX2022010521A (es) | 2020-02-28 | 2022-09-19 | Takeda Pharmaceuticals Co | Metodo para producir linfocitos citoliticos naturales a partir de celulas madre pluripotentes. |
CA3175071A1 (en) | 2020-03-11 | 2021-09-16 | Bit Bio Limited | Method of generating hepatic cells |
JP2023517112A (ja) | 2020-03-13 | 2023-04-21 | ゴーリヴァー・セラピューティクス | 劇症肝障害を処置及び/又は防止するための肝幹細胞様細胞 |
KR20220149592A (ko) | 2020-03-19 | 2022-11-08 | 오리즈루 세라퓨틱스 가부시키가이샤 | 심근세포 정제 방법 |
WO2021187602A1 (ja) | 2020-03-19 | 2021-09-23 | 国立大学法人京都大学 | 心筋細胞の精製方法 |
WO2021193360A1 (ja) | 2020-03-24 | 2021-09-30 | 株式会社カネカ | 膵臓α細胞への分化誘導方法 |
EP4129339A1 (en) | 2020-03-31 | 2023-02-08 | Sky Pharma Co., Ltd. | Method for screening for, method for producing, and method for designing drug active ingredients |
TW202204609A (zh) | 2020-03-31 | 2022-02-01 | 國立大學法人京都大學 | T前驅細胞的製造方法 |
WO2021241658A1 (ja) | 2020-05-26 | 2021-12-02 | 株式会社ヘリオス | 低免疫原性細胞 |
WO2021241668A1 (ja) | 2020-05-28 | 2021-12-02 | 武田薬品工業株式会社 | 均一なサイズの細胞凝集体の大量製造方法 |
WO2021243256A1 (en) | 2020-05-29 | 2021-12-02 | FUJIFILM Cellular Dynamics, Inc. | Retinal pigmented epithelium and photoreceptor dual cell aggregates and methods of use thereof |
JP2023536210A (ja) | 2020-05-29 | 2023-08-24 | フジフィルム セルラー ダイナミクス,インコーポレイテッド | 網膜色素上皮及び光受容体の二重層、並びにその使用 |
WO2021250058A2 (en) | 2020-06-12 | 2021-12-16 | Bayer Aktiengesellschaft | CRISPR-Cas12a DIRECTED RANDOM MUTAGENESIS AGENTS AND METHODS |
CN115885038A (zh) | 2020-06-17 | 2023-03-31 | 国立大学法人京都大学 | 表达嵌合抗原受体的免疫活性细胞 |
EP4180516A4 (en) | 2020-07-13 | 2024-01-17 | Kyoto University | SKELETON MUSCLE PRECURSOR CELLS AND METHOD FOR PURIFICATION THEREOF, COMPOSITION FOR THE TREATMENT OF MYOGENIC DISEASES AND METHOD FOR PRODUCING SKELETON MUSCLE PRECURSOR CELLS CONTAINING CELL GROUPS |
CN116134130A (zh) | 2020-07-20 | 2023-05-16 | 学校法人爱知医科大学 | 多能细胞的未分化维持培养用组合物、多能细胞的未分化维持培养用培养基、多能细胞的未分化状态下的维持培养方法、和多能细胞的制造方法 |
US20230265456A1 (en) | 2020-08-10 | 2023-08-24 | Fundacion Para La Investigacion Medica Aplicada | Gene therapy vector expressing cyp27a1 for the treatment of cerebrotendinous xanthomatosis |
CN116323917A (zh) | 2020-08-18 | 2023-06-23 | 国立大学法人京都大学 | 人原始生殖细胞/人原始生殖细胞样细胞的维持扩增方法 |
CA3200563A1 (en) | 2020-09-29 | 2022-04-07 | Genethon | Enhancing utrophin expression in cell by inducing mutations within utrophin regulatory elements and therapeutic use thereof |
EP4243839A1 (en) | 2020-11-13 | 2023-09-20 | Catamaran Bio, Inc. | Genetically modified natural killer cells and methods of use thereof |
EP4249587A1 (en) | 2020-11-20 | 2023-09-27 | Orizuru Therapeutics, Inc. | Maturation agent |
AU2021388155A1 (en) | 2020-11-25 | 2023-06-15 | Catamaran Bio, Inc. | Cellular therapeutics engineered with signal modulators and methods of use thereof |
KR20230125806A (ko) | 2020-12-16 | 2023-08-29 | 우니베르시타트 폼페우 파브라 | 선천성 근이영양증의 치료를 위한 치료용 lama2 페이로드 |
JPWO2022138101A1 (ja) | 2020-12-23 | 2022-06-30 | ||
EP4267197A1 (en) | 2020-12-23 | 2023-11-01 | Vivet Therapeutics | Minimal bile acid inducible promoters for gene therapy |
EP4269571A1 (en) | 2020-12-25 | 2023-11-01 | Kyoto University | Method for producing naive human ips cells from somatic cells |
JP7536245B2 (ja) | 2021-01-26 | 2024-08-20 | アイ ピース,インコーポレイテッド | オリゴデンドロサイトの作製方法 |
CN116829697A (zh) | 2021-02-09 | 2023-09-29 | 千纸鹤治疗公司 | 促熟剂 |
US20240158740A1 (en) | 2021-03-09 | 2024-05-16 | National University Corporation Tokyo Medical And Dental University | Cell cluster production method |
EP4310176A1 (en) | 2021-03-17 | 2024-01-24 | Astellas Pharma Inc. | Pericyte having basic fibroblast growth factor (bfgf) gene introduced therein |
WO2022194930A1 (en) | 2021-03-19 | 2022-09-22 | Technische Universität Dresden | Human macrophages resistant to tumor-induced repolarization |
EP4134086A1 (en) | 2021-08-12 | 2023-02-15 | Technische Universität Dresden | Human macrophages resistant to tumor-induced repolarization |
EP4060026A1 (en) | 2021-03-19 | 2022-09-21 | Technische Universität Dresden | Ex-vivo proliferation of human phagocytic cells |
WO2022207889A1 (en) | 2021-04-01 | 2022-10-06 | INSERM (Institut National de la Santé et de la Recherche Médicale) | Liver organoid manufacturing methods, liver organoids obtained with the same, and uses thereof |
US20240191192A1 (en) | 2021-04-08 | 2024-06-13 | Takeda Pharmaceutical Company Limited | Method for activating t-cells |
EP4332215A1 (en) | 2021-04-28 | 2024-03-06 | National University Corporation Tokyo Medical and Dental University | Method for producing cells |
TW202305112A (zh) | 2021-04-30 | 2023-02-01 | 國立研究開發法人理化學研究所 | 視網膜色素上皮細胞之線狀凝集體,用於製造其之裝置及製造方法,以及含有該線狀凝集體之治療藥 |
US20240252545A1 (en) | 2021-05-07 | 2024-08-01 | Children's Hospital Los Angeles | Methods for Making Stem Cell-Derived Enteric Neural Crest Cells and Their Use in Enteric Neuropathy Treatment |
EP4347796A1 (en) | 2021-05-26 | 2024-04-10 | Fujifilm Cellular Dynamics, Inc. | Methods to prevent rapid silencing of genes in pluripotent stem cells |
AU2022280062A1 (en) | 2021-05-28 | 2023-11-30 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Methods to generate macular, central and peripheral retinal pigment epithelial cells |
EP4346928A1 (en) | 2021-05-28 | 2024-04-10 | The United States of America, as represented by The Secretary, Department of Health and Human Services | Biodegradable tissue scaffold with secondary matrix to host weakly adherent cells |
US20240271099A1 (en) | 2021-06-07 | 2024-08-15 | Institut National de la Santé et de la Recherche Médicale | Method for generating highly functional hepatocytes by differentiating hepatoblasts |
JPWO2022259721A1 (ja) | 2021-06-10 | 2022-12-15 | ||
EP4101928A1 (en) | 2021-06-11 | 2022-12-14 | Bayer AG | Type v rna programmable endonuclease systems |
EP4352214A1 (en) | 2021-06-11 | 2024-04-17 | Bayer AG | Type v rna programmable endonuclease systems |
KR20240021878A (ko) | 2021-06-15 | 2024-02-19 | 다케다 야쿠힌 고교 가부시키가이샤 | 다능성 줄기 세포로부터 자연 살해 세포를 생산하기 위한 방법 |
JPWO2023277195A1 (ja) | 2021-06-29 | 2023-01-05 | ||
WO2023286832A1 (ja) | 2021-07-15 | 2023-01-19 | アステラス製薬株式会社 | 血管内皮増殖因子(vegf)高発現ペリサイト様細胞の製造方法 |
US20240335480A1 (en) | 2021-07-15 | 2024-10-10 | Astellas Pharma Inc. | Vascular endothelial growth factor (vegf)-highly expressing pericyte-like cell |
WO2023003025A1 (ja) | 2021-07-21 | 2023-01-26 | 国立大学法人京都大学 | 網膜組織の製造方法 |
JPWO2023017848A1 (ja) | 2021-08-11 | 2023-02-16 | ||
EP4144841A1 (en) | 2021-09-07 | 2023-03-08 | Bayer AG | Novel small rna programmable endonuclease systems with impoved pam specificity and uses thereof |
CA3231501A1 (en) | 2021-09-13 | 2023-03-16 | Steven Kattman | Methods for the production of committed cardiac progenitor cells |
TW202330910A (zh) | 2021-09-27 | 2023-08-01 | 國立大學法人京都大學 | T細胞的製造方法 |
WO2023053220A1 (ja) | 2021-09-28 | 2023-04-06 | 公益財団法人京都大学iPS細胞研究財団 | 多能性幹細胞の製造方法 |
EP4419655A1 (en) | 2021-10-20 | 2024-08-28 | University of Rochester | Humanized chimeras for the prospective assessment of cell addition and replacement therapies |
WO2023069881A1 (en) | 2021-10-20 | 2023-04-27 | University Of Rochester | Treatment with genetically modified cells, and genetically modified cells per se, with increased competitive advantage and/or decreased competitive disadvantage |
CA3234811A1 (en) | 2021-10-20 | 2023-04-27 | Steven Goldman | Rejuvenation treatment of age-related white matter loss |
WO2023070019A1 (en) | 2021-10-21 | 2023-04-27 | Vertex Pharmaceuticals Incorporated | Hypoimmune cells |
AU2022377078A1 (en) | 2021-11-01 | 2024-04-04 | Vertex Pharmaceuticals Incorporated | Stem cell derived pancreatic islet differentiation |
US20230270818A1 (en) | 2021-11-02 | 2023-08-31 | University Of Rochester | Tcf7l2 mediated remyelination in the brain |
KR20240099452A (ko) | 2021-11-11 | 2024-06-28 | 가부시키가이샤 헤리오스 | 유전자-변형 다능성 줄기 세포, 이로부터 유래하는 면역담당 세포, 상기 세포의 제조방법 및 이의 용도 |
EP4389902A1 (en) | 2021-11-15 | 2024-06-26 | National University Corporation Tottori University | Method for producing human artificial chromosome vector in human cells |
JPWO2023090361A1 (ja) | 2021-11-16 | 2023-05-25 | ||
JPWO2023090372A1 (ja) | 2021-11-16 | 2023-05-25 | ||
WO2023118068A1 (en) | 2021-12-23 | 2023-06-29 | Bayer Aktiengesellschaft | Novel small type v rna programmable endonuclease systems |
WO2023150557A1 (en) | 2022-02-01 | 2023-08-10 | University Of Rochester | Methods of generating a population of neurons from human glial progenitor cells and genetic constructs for carrying out such methods |
JPWO2023149555A1 (ja) | 2022-02-04 | 2023-08-10 | ||
CN118679244A (zh) | 2022-02-09 | 2024-09-20 | 住友制药株式会社 | 判定培养液中的细胞在由多能干细胞向中脑底板区域的神经系统细胞的分化中的分化能力的方法 |
WO2023157727A1 (ja) | 2022-02-15 | 2023-08-24 | 国立大学法人神戸大学 | ヒト多能性幹細胞由来ライディッヒ様細胞の作製方法及びヒト多能性幹細胞由来ライディッヒ様細胞集団 |
JP7315184B2 (ja) | 2022-02-16 | 2023-07-26 | 株式会社コーセー | 多能性幹細胞から表皮角化細胞への分化誘導方法 |
AU2023228602A1 (en) | 2022-03-02 | 2024-09-26 | Lineage Cell Therapeutics, Inc. | Methods and compositions for treating hearing loss |
WO2023172514A1 (en) | 2022-03-07 | 2023-09-14 | Catamaran Bio, Inc. | Engineered immune cell therapeutics targeted to her2 and methods of use thereof |
WO2023211857A1 (en) | 2022-04-25 | 2023-11-02 | Lineage Cell Therapeutics, Inc. | Methods and compositions for treating vision loss |
WO2023215455A1 (en) | 2022-05-05 | 2023-11-09 | University Of Rochester | Dual macroglial-microglial approach towards therapeutic cell replacement in neurodegenerative and neuropsychiatric disease |
WO2023237587A1 (en) | 2022-06-10 | 2023-12-14 | Bayer Aktiengesellschaft | Novel small type v rna programmable endonuclease systems |
WO2023247532A1 (en) | 2022-06-21 | 2023-12-28 | Institut National de la Santé et de la Recherche Médicale | A method for producing a bioengineered mammal induced pluripotent stem cell-derived cardiac organoid |
WO2024006911A1 (en) | 2022-06-29 | 2024-01-04 | FUJIFILM Holdings America Corporation | Ipsc-derived astrocytes and methods of use thereof |
EP4338745A1 (en) | 2022-09-14 | 2024-03-20 | Technische Universität Dresden | Allogeneic human macrophages for cell therapy |
WO2024073776A1 (en) | 2022-09-30 | 2024-04-04 | FUJIFILM Cellular Dynamics, Inc. | Methods for the production of cardiac fibroblasts |
WO2024129743A2 (en) | 2022-12-13 | 2024-06-20 | Bluerock Therapeutics Lp | Engineered type v rna programmable endonucleases and their uses |
WO2024163747A2 (en) | 2023-02-02 | 2024-08-08 | University Of Rochester | Competitive replacement of glial cells |
WO2024167814A1 (en) | 2023-02-06 | 2024-08-15 | Bluerock Therapeutics Lp | Degron fusion proteins and methods of production and use thereof |
WO2024192329A1 (en) | 2023-03-16 | 2024-09-19 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Methods for producing stable human chondroctyes and their use for promoting cartillage growth and repair |
Family Cites Families (157)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US70292A (en) * | 1867-10-29 | Petess | ||
US4650761A (en) * | 1981-11-27 | 1987-03-17 | Eli Lilly And Company | Method for stabilizing and selecting recombinant DNA containing host cell |
US4650764A (en) | 1983-04-12 | 1987-03-17 | Wisconsin Alumni Research Foundation | Helper cell |
US4861719A (en) | 1986-04-25 | 1989-08-29 | Fred Hutchinson Cancer Research Center | DNA constructs for retrovirus packaging cell lines |
US4937190A (en) | 1987-10-15 | 1990-06-26 | Wisconsin Alumni Research Foundation | Translation enhancer |
US5192553A (en) | 1987-11-12 | 1993-03-09 | Biocyte Corporation | Isolation and preservation of fetal and neonatal hematopoietic stem and progenitor cells of the blood and methods of therapeutic use |
US6140111A (en) | 1987-12-11 | 2000-10-31 | Whitehead Institute For Biomedical Research | Retroviral gene therapy vectors and therapeutic methods based thereon |
US5591624A (en) | 1988-03-21 | 1997-01-07 | Chiron Viagene, Inc. | Retroviral packaging cell lines |
US7070994B2 (en) | 1988-03-21 | 2006-07-04 | Oxford Biomedica (Uk) Ltd. | Packaging cells |
JP2886547B2 (ja) | 1988-07-26 | 1999-04-26 | 協和醗酵工業株式会社 | ノイラミニダーゼの製造法 |
EP0432216A1 (en) | 1988-09-01 | 1991-06-19 | Whitehead Institute For Biomedical Research | Recombinant retroviruses with amphotropic and ecotropic host ranges |
US5266491A (en) | 1989-03-14 | 1993-11-30 | Mochida Pharmaceutical Co., Ltd. | DNA fragment and expression plasmid containing the DNA fragment |
JP3051411B2 (ja) | 1989-03-14 | 2000-06-12 | 持田製薬株式会社 | 新規dnaならびにそれを含有する発現プラスミド |
JP2897295B2 (ja) | 1989-12-14 | 1999-05-31 | 味の素株式会社 | レトロウィルス高生産用dna構築物及びレトロウィルス高生産用細胞株 |
US5652122A (en) | 1989-12-21 | 1997-07-29 | Frankel; Alan | Nucleic acids encoding and methods of making tat-derived transport polypeptides |
US5817491A (en) | 1990-09-21 | 1998-10-06 | The Regents Of The University Of California | VSV G pseusdotyped retroviral vectors |
US5288514A (en) | 1992-09-14 | 1994-02-22 | The Regents Of The University Of California | Solid phase and combinatorial synthesis of benzodiazepine compounds on a solid support |
US5834256A (en) | 1993-06-11 | 1998-11-10 | Cell Genesys, Inc. | Method for production of high titer virus and high efficiency retroviral mediated transduction of mammalian cells |
FR2707091B1 (fr) | 1993-06-30 | 1997-04-04 | Cohen Haguenauer Odile | Vecteur rétroviral pour le transfert et l'expression de gènes dans des cellules eucaryotes. |
US5534423A (en) | 1993-10-08 | 1996-07-09 | Regents Of The University Of Michigan | Methods of increasing rates of infection by directing motion of vectors |
US5519134A (en) | 1994-01-11 | 1996-05-21 | Isis Pharmaceuticals, Inc. | Pyrrolidine-containing monomers and oligomers |
US6013517A (en) | 1994-05-09 | 2000-01-11 | Chiron Corporation | Crossless retroviral vectors |
US5525735A (en) | 1994-06-22 | 1996-06-11 | Affymax Technologies Nv | Methods for synthesizing diverse collections of pyrrolidine compounds |
US5549974A (en) | 1994-06-23 | 1996-08-27 | Affymax Technologies Nv | Methods for the solid phase synthesis of thiazolidinones, metathiazanones, and derivatives thereof |
DK0787200T3 (da) | 1994-10-28 | 2005-08-15 | Univ Pennsylvania | Forbedret adenovirus og fremgangsmåder til anvendelse heraf |
US5843780A (en) | 1995-01-20 | 1998-12-01 | Wisconsin Alumni Research Foundation | Primate embryonic stem cells |
US5637456A (en) | 1995-02-17 | 1997-06-10 | The University Of Texas, Board Of Regents | Rapid test for determining the amount of functionally inactive gene in a gene therapy vector preparation |
US5707618A (en) | 1995-03-24 | 1998-01-13 | Genzyme Corporation | Adenovirus vectors for gene therapy |
US5830725A (en) | 1995-04-28 | 1998-11-03 | The Board Of Trustees For The Leland Stanford Junior University | Rapid, stable high-titre production of recombing retrovirus |
US5744320A (en) | 1995-06-07 | 1998-04-28 | Promega Corporation | Quenching reagents and assays for enzyme-mediated luminescence |
EP0845043B1 (en) | 1995-07-28 | 2007-06-27 | Marie Curie Cancer Care | Transport proteins and their uses |
WO1997011083A1 (en) | 1995-09-22 | 1997-03-27 | Medical Research Council | Improvements in or relating to mutagenesis of nucleic acids |
US5910434A (en) | 1995-12-15 | 1999-06-08 | Systemix, Inc. | Method for obtaining retroviral packaging cell lines producing high transducing efficiency retroviral supernatant |
FR2751345B1 (fr) | 1996-07-16 | 1998-09-18 | Univ Paris Curie | Lignees d'encapsidation hautement productrices |
US6025192A (en) | 1996-09-20 | 2000-02-15 | Cold Spring Harbor Laboratory | Modified retroviral vectors |
US6255071B1 (en) | 1996-09-20 | 2001-07-03 | Cold Spring Harbor Laboratory | Mammalian viral vectors and their uses |
US6017735A (en) | 1997-01-23 | 2000-01-25 | Marie Curie Cancer Care | Materials and methods for intracellular transport and their uses |
US6416959B1 (en) | 1997-02-27 | 2002-07-09 | Kenneth Giuliano | System for cell-based screening |
AU9200398A (en) | 1997-08-22 | 1999-03-16 | Yale University | A process to study changes in gene expression in granulocytic cells |
JPH11115328A (ja) * | 1997-10-16 | 1999-04-27 | Dainippon Printing Co Ltd | 熱転写受像シート及びその製造方法 |
US6835567B1 (en) * | 1998-04-14 | 2004-12-28 | Signal Pharmaceuticals, Inc. | PNS cell lines and methods of use therefor |
US20020174013A1 (en) | 1998-04-17 | 2002-11-21 | Viztec Inc., A Florida Corporation | Chip card advertising method and system |
EP1080218A1 (en) | 1998-05-27 | 2001-03-07 | University of Florida | Method of preparing recombinant adeno-associated virus compositions by using an iodixanol gradient |
KR20000006334A (ko) | 1998-06-26 | 2000-01-25 | 이선경 | 바이러스코딩염기서열이전혀없는고효율레트로바이러스벡터 |
US6485959B1 (en) | 1998-10-07 | 2002-11-26 | Cedars Sinai Medical Center | Cell preconditioning and cryopresevation medium |
CA2346152A1 (en) | 1998-10-16 | 2000-04-27 | Novartis Ag | Promotion of self-renewal and improved gene transduction of hematopoietic stem cells by histone deacetylase inhibitors |
US6667176B1 (en) | 2000-01-11 | 2003-12-23 | Geron Corporation | cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells |
WO2000027995A1 (en) | 1998-11-09 | 2000-05-18 | Monash University | Embryonic stem cells |
US6376246B1 (en) | 1999-02-05 | 2002-04-23 | Maxygen, Inc. | Oligonucleotide mediated nucleic acid recombination |
US6153432A (en) | 1999-01-29 | 2000-11-28 | Zen-Bio, Inc | Methods for the differentiation of human preadipocytes into adipocytes |
US6312949B1 (en) | 1999-03-26 | 2001-11-06 | The Salk Institute For Biological Studies | Regulation of tyrosine hydroxylase expression |
US6773920B1 (en) | 1999-03-31 | 2004-08-10 | Invitrogen Corporation | Delivery of functional protein sequences by translocating polypeptides |
WO2000073423A1 (fr) | 1999-06-01 | 2000-12-07 | Chugai Seiyaku Kabushiki Kaisha | Cellule de conditionnement |
US7015037B1 (en) | 1999-08-05 | 2006-03-21 | Regents Of The University Of Minnesota | Multiponent adult stem cells and methods for isolation |
WO2001015511A2 (en) | 1999-09-01 | 2001-03-08 | University Of Pittsburgh Of The Commonwealth System Of Higher Education | Identification of peptides that facilitate uptake and cytoplasmic and/or nuclear transport of proteins, dna and viruses |
WO2001021767A2 (en) | 1999-09-24 | 2001-03-29 | Morphogen Pharmaceuticals, Inc. | Pluripotent embryonic-like stem cells, compositions, methods and uses thereof |
US20030161817A1 (en) | 2001-03-28 | 2003-08-28 | Young Henry E. | Pluripotent embryonic-like stem cells, compositions, methods and uses thereof |
US6280718B1 (en) | 1999-11-08 | 2001-08-28 | Wisconsin Alumni Reasearch Foundation | Hematopoietic differentiation of human pluripotent embryonic stem cells |
US7544509B2 (en) | 2000-01-24 | 2009-06-09 | Mcgill University | Method for preparing stem cell preparations |
US6395546B1 (en) | 2000-02-01 | 2002-05-28 | Neurogeneration, Inc. | Generation of dopaminergic neurons from human nervous system stem cells |
US7439064B2 (en) | 2000-03-09 | 2008-10-21 | Wicell Research Institute, Inc. | Cultivation of human embryonic stem cells in the absence of feeder cells or without conditioned medium |
US6458589B1 (en) | 2000-04-27 | 2002-10-01 | Geron Corporation | Hepatocyte lineage cells derived from pluripotent stem cells |
JP5943533B2 (ja) | 2000-05-17 | 2016-07-06 | アステリアス バイオセラピューティクス インコーポレイテッド | 神経前駆細胞の集団 |
WO2001096532A2 (en) | 2000-06-15 | 2001-12-20 | Tanja Dominko | Method of generating pluripotent mammalian cells by fusion of a cytoplast fragment with a karyoplast |
DE10031179A1 (de) | 2000-06-27 | 2002-01-31 | Amaxa Gmbh | Verfahren zur Einbringung von Nukleinsäuren und anderen biologisch aktiven Molekülen in den Kern höherer eukaryontischer Zellen mit Hilfe elektrischen Stroms |
EP1305333A4 (en) | 2000-07-31 | 2006-04-12 | Active Motif | ADMINISTRATION OF MOLECULES IN CELLS BY PEPTIDE MEDIATION |
JP2002065261A (ja) | 2000-08-30 | 2002-03-05 | Mitsubishi Kasei Institute Of Life Sciences | 生殖細胞の取得方法 |
US6910434B2 (en) | 2000-08-31 | 2005-06-28 | Edwin Lundgren | Control device for steering kite on a boat |
WO2002061033A2 (en) | 2000-11-27 | 2002-08-08 | Yissum Research Development Company Of The Hebrew University Of Jerusalem | Transfection of human embryonic stem cells |
US20080268054A1 (en) | 2000-12-04 | 2008-10-30 | Eugene Bell | Dermal derived human stem cells and compositions and methods thereof |
CA2433419A1 (en) | 2001-01-02 | 2002-07-25 | Stemron, Inc. | A method for producing a population of homozygous stem cells having a pre-selected immunotype and/or genotype, cells suitable for transplant derived therefrom, and materials and methods using same |
CA2434281A1 (en) * | 2001-01-31 | 2002-08-08 | Interface Biotech A/S | An improved in vitro method of culturing mammalian cells for autologous cell implantation/transplantation methods |
JP2003009854A (ja) | 2001-04-09 | 2003-01-14 | Kyowa Hakko Kogyo Co Ltd | エンブリオイドボディ形成方法及びその用途 |
AU2002315658B2 (en) | 2001-04-23 | 2007-02-08 | Lonza Cologne Gmbh | Buffer solution for electroporation and a method comprising the use of the same |
DE10119901A1 (de) | 2001-04-23 | 2002-10-24 | Amaxa Gmbh | Schaltungsanordnung zur Einbringung von Nukleinsäuren und anderen biologisch aktiven Molekülen in den Kern höherer eukaryontischer Zellen mit Hilfe elektrischen Stroms |
ES2544854T3 (es) | 2001-05-31 | 2015-09-04 | Shinya Yamanaka | Genes con expresión específica de células ES |
WO2003018780A1 (en) | 2001-08-27 | 2003-03-06 | Advanced Cell Technology, Inc. | De-differentiation and re-differentiation of somatic cells and production of cells for cell therapies |
JPWO2003027281A1 (ja) | 2001-09-20 | 2005-01-06 | 協和醗酵工業株式会社 | 骨格筋間質由来多分化能幹細胞 |
JP2004248505A (ja) | 2001-09-21 | 2004-09-09 | Norio Nakatsuji | 移植抗原の一部または全てを欠除したes細胞由来の未分化な体細胞融合細胞およびその製造 |
EP1437403A4 (en) | 2001-09-21 | 2004-10-27 | Japan Science & Tech Corp | METHOD FOR PATTERNING REPROGRAMMING FACTOR, METHOD OF PATTERNED REPROGRAMMING FACTOR, METHOD FOR USE OF REPROGRAMMING FACTOR, METHOD FOR DIFFERENTIATING AND SIFE-INGENATING FAN |
US7588937B2 (en) | 2001-10-03 | 2009-09-15 | Wisconsin Alumni Research Foundation | Method of in vitro differentiation of neural stem cells, motor neurons and dopamine neurons from primate embryonic stem cells |
DE10162080A1 (de) | 2001-12-10 | 2003-06-26 | Albrecht Mueller | Verfahren zur Herstellung von Stammzellen mit erhöhtem Entwicklungspotential |
IL162648A0 (en) * | 2001-12-21 | 2005-11-20 | Mount Sinai Hospital Corp | Cellular compositions and methods of making and using them |
EP1471140A4 (en) | 2002-01-31 | 2005-02-16 | Asahi Techno Glass Cosporation | LIQUIDITY FOR DEEP-FREEZE STORAGE OF EMBRYONIC PRIMATIVE STEM CELLS AND DEEP-FREEZE STORAGE PROCESS |
WO2003068937A2 (en) * | 2002-02-13 | 2003-08-21 | Anthrogenesis Corporation | Embryonic-like stem cells derived from post-partum mammalian placenta and uses and methods of treatment using said cells |
ES2198216B1 (es) * | 2002-07-02 | 2005-04-16 | Juan Carlos Instituto Cientifico Y Tecnologico De Navarra, S.A.(67%). | Medio de cultivo de celulas madre-progenitoras autologas humanas y sus aplicaciones. |
US7422736B2 (en) | 2002-07-26 | 2008-09-09 | Food Industry Research And Development Institute | Somatic pluripotent cells |
US20040048297A1 (en) | 2002-07-30 | 2004-03-11 | Gene Logic, Inc. | Nucleic acid detection assay control genes |
JP3736517B2 (ja) | 2002-11-13 | 2006-01-18 | 学校法人近畿大学 | 体細胞核初期化因子 |
AU2003901099A0 (en) | 2003-03-11 | 2003-03-27 | Es Cell International Pte Ltd. | Methods of inducing differentiation of stem cells |
US20070104679A1 (en) * | 2003-03-25 | 2007-05-10 | Masuo Obinata | Induction of differentiation of stem cells, and control of differentiation potency of stem cells |
CN1536076A (zh) * | 2003-04-09 | 2004-10-13 | 中国人民解放军军事医学科学院野战输 | 成年人骨髓间充质干细胞体外扩增和定向诱导分化为心肌样细胞的方法 |
US9567591B2 (en) | 2003-05-15 | 2017-02-14 | Mello Biotechnology, Inc. | Generation of human embryonic stem-like cells using intronic RNA |
JPWO2004101775A1 (ja) | 2003-05-16 | 2006-07-13 | 協和醗酵工業株式会社 | 新規な成体組織由来の幹細胞およびその用途 |
WO2005010524A1 (en) | 2003-06-04 | 2005-02-03 | Curis, Inc. | Stem cell-based methods for identifying and characterizing agents |
FR2859219B1 (fr) * | 2003-09-02 | 2005-10-14 | Alain Privat | Procede de production de neurones a partir de cellules d'une lignee cellulaire |
JP2005095027A (ja) | 2003-09-22 | 2005-04-14 | Reprocell Inc | 細胞の未分化状態マーカープロモーターおよびその利用 |
AU2004280066A1 (en) | 2003-10-09 | 2005-04-21 | Kyowa Hakko Kirin Co., Ltd. | Genomically modified cell |
JP4976852B2 (ja) | 2003-11-10 | 2012-07-18 | ザ スクリプス リサーチ インスティチュート | 細胞脱分化を誘導するための組成物および方法 |
US7682828B2 (en) | 2003-11-26 | 2010-03-23 | Whitehead Institute For Biomedical Research | Methods for reprogramming somatic cells |
WO2005053601A2 (en) | 2003-12-01 | 2005-06-16 | Technion Research & Development Foundation Ltd. | Methods of generating stem cells and embryonic bodies carrying disease-causing mutations and methods of using same for studying genetic disorders |
WO2005065354A2 (en) * | 2003-12-31 | 2005-07-21 | The Burnham Institute | Defined media for pluripotent stem cell culture |
JP4901471B2 (ja) | 2004-02-19 | 2012-03-21 | 国立大学法人京都大学 | 体細胞核初期化物質のスクリーニング方法 |
RU2375448C2 (ru) | 2004-03-23 | 2009-12-10 | Асубио Фарма Ко., Лтд. | Способ выращивания плюрипотентных стволовых клеток |
CA2561690A1 (en) * | 2004-03-30 | 2005-10-27 | Kyoto University | Process for producing multipotential stem cell originating in testoid cell |
WO2005117557A2 (en) | 2004-06-01 | 2005-12-15 | San Diego State University Foundation | Expression system |
US20070202592A1 (en) | 2004-07-08 | 2007-08-30 | Yasuo Kitagawa | Pluripotent Cells Distributed Ubiquitously In Animal Tissue, Which Proliferate Selectively In Lower-Serum Culture |
WO2006084229A2 (en) | 2004-07-15 | 2006-08-10 | Primegen Biotech, Llc | Use of nuclear material to therapeutically reprogram differentiated cells |
US20060088599A1 (en) | 2004-08-02 | 2006-04-27 | Prasad Paras N | Amino functionalized ORMOSIL nanoparticles as delivery vehicles |
US7803920B2 (en) | 2004-09-29 | 2010-09-28 | Shinya Yamanaka | ECAT16 gene expressed specifically in ES cells and utilization of the same |
US20060095319A1 (en) | 2004-10-29 | 2006-05-04 | Cardwell Carlzo B | Marketing and compensation method |
US20060182724A1 (en) | 2005-02-15 | 2006-08-17 | Riordan Neil H | Method for expansion of stem cells |
WO2006093172A1 (ja) | 2005-02-28 | 2006-09-08 | Foundation For Biomedical Research And Innovation | 成体幹細胞の体外増幅方法 |
US20070033061A1 (en) | 2005-04-05 | 2007-02-08 | Achaogen, Inc. | Business methods for commercializing antimicrobial and cytotoxic compounds |
WO2007026255A2 (en) | 2005-06-22 | 2007-03-08 | Universitetet I Oslo | Dedifferentiated cells and methods of making and using dedifferentiated cells |
WO2007016566A2 (en) * | 2005-08-01 | 2007-02-08 | Nupotential, Llc | Production of reprogrammed cells with restored potential |
JP2009515515A (ja) | 2005-11-11 | 2009-04-16 | ザ・ユニバーシティ・コート・オブ・ザ・ユニバーシティ・オブ・エディンバラ | 細胞の再プログラム化および遺伝子改変 |
US8278104B2 (en) | 2005-12-13 | 2012-10-02 | Kyoto University | Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2 |
US8129187B2 (en) | 2005-12-13 | 2012-03-06 | Kyoto University | Somatic cell reprogramming by retroviral vectors encoding Oct3/4. Klf4, c-Myc and Sox2 |
US20090227032A1 (en) | 2005-12-13 | 2009-09-10 | Kyoto University | Nuclear reprogramming factor and induced pluripotent stem cells |
EP1970446B1 (en) | 2005-12-13 | 2011-08-03 | Kyoto University | Nuclear reprogramming factor |
CN101389761A (zh) | 2006-02-27 | 2009-03-18 | 银怎株式会社 | 使用bmi-1使星形胶质细胞去分化成为神经干细胞 |
US20090252711A1 (en) | 2006-05-11 | 2009-10-08 | Andrew Craig Boquest | Stem Cells And Methods Of Making And Using Stem Cells |
US20090028835A1 (en) | 2006-09-08 | 2009-01-29 | Michigan State University | Human transcriptome corresponding to human oocytes and use of said genes or the corresponding polypeptides to trans-differentiate somatic cells |
JP2008099662A (ja) | 2006-09-22 | 2008-05-01 | Institute Of Physical & Chemical Research | 幹細胞の培養方法 |
US20080132803A1 (en) | 2006-11-30 | 2008-06-05 | Hyman Friedlander | Method and system for doing business by mining the placental-chord complex |
WO2008089351A1 (en) | 2007-01-17 | 2008-07-24 | Wisconsin Alumni Research Foundation | Improved culture of stem cells |
WO2008105630A1 (en) | 2007-02-27 | 2008-09-04 | Procell Therapeutics Inc. | Combined use of cell permeable nanog and oct4 for increasing self-renewal and suppressing differentiation of stem cells |
WO2008105566A1 (en) | 2007-02-27 | 2008-09-04 | Korea Stem Cell Bank | System for providing stem cell services using internet and method thereof |
SG193653A1 (en) | 2007-03-23 | 2013-10-30 | Wisconsin Alumni Res Found | Somatic cell reprogramming |
EP2145000A4 (en) | 2007-04-07 | 2010-05-05 | Whitehead Biomedical Inst | REPROGRAMMING SOMATIC CELLS |
SG10201903161XA (en) | 2007-05-29 | 2019-05-30 | Christopher Reid | Methods for production and uses of multipotent cell populations |
EP2164951A2 (en) | 2007-05-30 | 2010-03-24 | The General Hospital Corporation | Methods of generating pluripotent cells from somatic cells |
US9213999B2 (en) | 2007-06-15 | 2015-12-15 | Kyoto University | Providing iPSCs to a customer |
JP2008307007A (ja) | 2007-06-15 | 2008-12-25 | Bayer Schering Pharma Ag | 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞 |
WO2009032456A2 (en) | 2007-08-01 | 2009-03-12 | Primegen Biotech Llc | Non-viral delivery of transcription factors that reprogram human somatic cells into a stem cell-like state |
EP2190976A4 (en) | 2007-08-10 | 2010-10-20 | Univ Dayton | METHOD FOR PRODUCING PLURIPOTENTAL STEM CELL LENGTH CELLS |
CN101855338B (zh) | 2007-08-31 | 2013-07-17 | 怀特黑德生物医学研究所 | 在程序重排体细胞中的wnt途径刺激 |
EP2096169B1 (en) | 2007-10-31 | 2020-11-18 | Kyoto University | Nuclear reprogramming method |
WO2009061442A1 (en) | 2007-11-06 | 2009-05-14 | Children's Medical Center Corporation | Method to produce induced pluripotent stem (ips) cells form non-embryonic human cells |
WO2009067563A1 (en) | 2007-11-19 | 2009-05-28 | The Regents Of The University Of California | Generation of pluripotent cells from fibroblasts |
JP5558097B2 (ja) | 2007-12-10 | 2014-07-23 | 国立大学法人京都大学 | 効率的な核初期化方法 |
US9683232B2 (en) | 2007-12-10 | 2017-06-20 | Kyoto University | Efficient method for nuclear reprogramming |
US20090191171A1 (en) | 2008-01-18 | 2009-07-30 | Yupo Ma | Reprogramming of Differentiated Progenitor or Somatic Cells Using Homologous Recombination |
KR101481164B1 (ko) | 2008-01-30 | 2015-01-09 | 주식회사 미래셀바이오 | 체세포 유래 다능성 줄기세포의 제조 방법 |
US20110014164A1 (en) | 2008-02-15 | 2011-01-20 | President And Fellows Of Harvard College | Efficient induction of pluripotent stem cells using small molecule compounds |
RU2010139426A (ru) | 2008-03-17 | 2012-04-27 | Зе Скрипс Ресеч Инститьют (Us) | Комбинация химического и генетического подходов к получению индуцированных плюрипотентных стволовых клеток |
JP2011516042A (ja) | 2008-03-17 | 2011-05-26 | へルムホルツ・ツェントルム・ミュンヘン−ドイチェス・フォルシュングスツェントルム・ヒューア・ゲズントハイト・ウント・ウムヴェルト(ゲゼルシャフト・ミット・ベシュレンクテル・ハフツング) | 部位特異的組み換えを用いて無ベクター誘導多能性幹(iPS)細胞を作製するベクターおよびその方法 |
CN101250502A (zh) | 2008-04-01 | 2008-08-27 | 中国科学院上海生命科学研究院 | 一种诱导的多潜能干细胞的制备方法 |
CN101550406B (zh) | 2008-04-03 | 2016-02-10 | 北京大学 | 制备多潜能干细胞的方法,试剂盒及用途 |
US20100021437A1 (en) | 2008-04-07 | 2010-01-28 | The McLean Hospital Corporation Whitehead Institute for Biomedical Research | Neural stem cells derived from induced pluripotent stem cells |
EP2268809B1 (en) | 2008-05-02 | 2019-02-06 | Kyoto University | Method of nuclear reprogramming |
EP2128245A1 (en) | 2008-05-27 | 2009-12-02 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Generation of induced pluripotent stem (iPS) cells |
US8546140B2 (en) | 2008-06-04 | 2013-10-01 | Cellular Dynamics International, Inc. | Methods for the production of iPS cells using non-viral approach |
WO2010013359A1 (en) | 2008-07-31 | 2010-02-04 | Gifu University | Efficient method for establishing induced pluripotent stem cells |
US20100062534A1 (en) | 2008-09-09 | 2010-03-11 | The General Hospital Corporation | Inducible lentiviral vectors for reprogramming somatic cells |
US8268620B2 (en) | 2008-10-24 | 2012-09-18 | Wisconsin Alumni Research Foundation | OCT4 and SOX2 with SV40 T antigen produce pluripotent stem cells from primate somatic cells |
-
2006
- 2006-12-06 EP EP06834636A patent/EP1970446B1/en active Active
- 2006-12-06 MX MX2008007654A patent/MX2008007654A/es active IP Right Grant
- 2006-12-06 CN CN201310015158.1A patent/CN103113463B/zh active Active
- 2006-12-06 CN CN200680048227.7A patent/CN101356270B/zh active Active
- 2006-12-06 CA CA2632142A patent/CA2632142C/en active Active
- 2006-12-06 WO PCT/JP2006/324881 patent/WO2007069666A1/ja active Application Filing
- 2006-12-06 BR BRPI0619794A patent/BRPI0619794B8/pt active IP Right Grant
- 2006-12-06 CN CN201410006027.1A patent/CN103773804A/zh active Pending
- 2006-12-06 US US12/086,479 patent/US8048999B2/en active Active
- 2006-12-06 EA EA200870046A patent/EA014166B1/ru not_active IP Right Cessation
- 2006-12-06 JP JP2007550210A patent/JP5098028B2/ja active Active
- 2006-12-06 EP EP10154819A patent/EP2206724A1/en not_active Withdrawn
- 2006-12-06 NZ NZ569530A patent/NZ569530A/en unknown
- 2006-12-06 EP EP18174008.5A patent/EP3418297B1/en active Active
- 2006-12-06 MX MX2011013772A patent/MX352337B/es unknown
- 2006-12-06 EP EP10154817.0A patent/EP2208786B1/en active Active
- 2006-12-06 ES ES06834636T patent/ES2367525T3/es active Active
- 2006-12-06 EA EA201000858A patent/EA018039B1/ru not_active IP Right Cessation
- 2006-12-06 KR KR1020087017015A patent/KR101420740B1/ko active IP Right Grant
- 2006-12-06 DK DK06834636.0T patent/DK1970446T3/da active
- 2006-12-06 AU AU2006325975A patent/AU2006325975B2/en active Active
- 2006-12-06 EP EP23165497.1A patent/EP4223769A3/en active Pending
- 2006-12-06 CN CN201010126185.2A patent/CN101864392B/zh active Active
- 2006-12-06 PT PT06834636T patent/PT1970446E/pt unknown
- 2006-12-06 ZA ZA200804673A patent/ZA200804673B/xx unknown
- 2006-12-06 EP EP10154821.2A patent/EP2206778B1/en active Active
-
2008
- 2008-05-20 JP JP2008131577A patent/JP4183742B1/ja active Active
- 2008-06-03 IL IL191903A patent/IL191903A/en active IP Right Grant
-
2009
- 2009-03-10 JP JP2009056747A patent/JP4411362B2/ja active Active
- 2009-03-10 JP JP2009056748A patent/JP5248371B2/ja active Active
- 2009-03-10 JP JP2009056750A patent/JP4411363B2/ja active Active
- 2009-03-10 JP JP2009056749A patent/JP5467223B2/ja active Active
- 2009-03-12 HK HK09102406.5A patent/HK1125131A1/xx unknown
- 2009-04-17 HK HK09103541.9A patent/HK1125967A1/xx unknown
-
2011
- 2011-04-12 JP JP2011088113A patent/JP5603282B2/ja active Active
-
2013
- 2013-08-12 JP JP2013167725A patent/JP5943324B2/ja active Active
Cited By (49)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2010119819A1 (ja) | 2009-04-17 | 2010-10-21 | 国立大学法人東北大学 | ヒト肺組織幹細胞の調製方法及びヒト肺胞上皮細胞への分化誘導方法 |
KR20150063168A (ko) | 2009-07-15 | 2015-06-08 | 마리 데자와 | 생체조직으로부터 단리 할 수 있는 다능성 줄기세포 |
EP3680323A1 (en) | 2009-07-15 | 2020-07-15 | Mari Dezawa | Pluripotent stem cell that can be isolated from body tissue |
EP3214170A1 (en) | 2009-07-15 | 2017-09-06 | Mari Dezawa | Pluripotent stem cell that can be isolated from body tissue |
WO2011024550A1 (ja) * | 2009-08-31 | 2011-03-03 | 国立大学法人大阪大学 | 口腔粘膜由来細胞を利用した誘導多能性幹細胞の効率的な製造方法 |
US8748179B2 (en) | 2009-08-31 | 2014-06-10 | Osaka University | Method for efficient production of induced pluripotent stem cells utilizing cells derived from oral mucosa |
WO2014027684A1 (ja) | 2012-08-17 | 2014-02-20 | 株式会社Clio | 心筋梗塞の修復再生を誘導する多能性幹細胞 |
EP3659612A1 (en) | 2012-08-17 | 2020-06-03 | Clio, Inc. | Pluripotent stem cell that induces repair and regeneration after myocardial infarction technical field |
WO2014133170A1 (ja) | 2013-03-01 | 2014-09-04 | 株式会社Clio | 多能性幹細胞を損傷部位に誘導する遊走因子を含む医薬組成物 |
US11000552B2 (en) | 2014-09-05 | 2021-05-11 | The University Of Tokyo | Pluripotent stem cell for treating diabetic skin ulcer |
KR20170055477A (ko) | 2014-09-05 | 2017-05-19 | 고쿠리츠다이가쿠호우진 도쿄다이가쿠 | 당뇨병성 피부 궤양 치료를 위한 다능성 줄기 세포 |
EP3366763A2 (en) | 2015-08-31 | 2018-08-29 | I Peace, Inc. | Device for cells dissociation |
US11912977B2 (en) | 2015-08-31 | 2024-02-27 | I Peace, Inc. | Pluripotent stem cell production system |
US11518974B2 (en) | 2015-08-31 | 2022-12-06 | I Peace, Inc. | Pluripotent stem cell production system |
US11286454B2 (en) | 2015-08-31 | 2022-03-29 | I Peace, Inc. | Pluripotent stem cell manufacturing system and method for producing induced pluripotent stem cells |
EP3473703A2 (en) | 2015-08-31 | 2019-04-24 | I Peace, Inc. | Pluripotent stem cell production system |
US10508260B2 (en) | 2015-08-31 | 2019-12-17 | I Peace, Inc. | Pluripotent stem cell production system |
WO2017040548A1 (en) | 2015-08-31 | 2017-03-09 | I Peace, Inc. | Pluripotent stem cell manufacturing system and method for producing induced pluripotent stem cells |
KR20200127039A (ko) | 2016-05-16 | 2020-11-09 | 고쿠리츠 다이가쿠 호우징 도우카이 고쿠리츠 다이가쿠 기코우 | 다능성 간세포에 의한 주산기 뇌장애의 개선 및 치료 |
KR20190039102A (ko) | 2016-08-03 | 2019-04-10 | 고쿠리츠 다이가쿠 호우징 나고야 다이가쿠 | 다능성 간세포에 따른 만성폐질환의 개선 및 치료 |
KR20190039700A (ko) | 2016-08-03 | 2019-04-15 | 가부시키가이샤 세이메이카가쿠 인스티튜트 | 다능성 간세포에 의한 허혈재관류 폐장애의 경감 및 치료 |
DE102017212851A1 (de) | 2016-08-04 | 2018-02-08 | Fanuc Corporation | Stammzellenherstellungssystem, Stammzelleninformationsverwaltungssystem, Zellentransportvorrichtung und Speichervorrichtung für gefrorene Stammzellen |
WO2018155607A1 (ja) | 2017-02-24 | 2018-08-30 | 剛士 田邊 | 細胞処理装置、浮遊培養器、及び幹細胞の誘導方法 |
US11959058B2 (en) | 2017-02-27 | 2024-04-16 | I Peace, Inc. | Cell processing system and cell processing device |
WO2018154788A1 (ja) | 2017-02-27 | 2018-08-30 | 剛士 田邊 | 体細胞製造システム |
WO2018154791A1 (ja) | 2017-02-27 | 2018-08-30 | 剛士 田邊 | 細胞処理システム及び細胞処理装置 |
WO2018203499A1 (ja) | 2017-05-02 | 2018-11-08 | 剛士 田邊 | 医薬品組成物及び化粧品組成物 |
KR20200016871A (ko) | 2017-06-20 | 2020-02-17 | 고쿠리츠 다이가쿠 호우징 나고야 다이가쿠 | 다능성 간세포에 의한 태아발육부전에 따르는 뇌장애의 개선 및 치료 |
WO2018235878A1 (ja) | 2017-06-20 | 2018-12-27 | 国立大学法人名古屋大学 | 多能性幹細胞による胎児発育不全に伴う脳障害の改善及び治療 |
WO2019078262A1 (ja) | 2017-10-17 | 2019-04-25 | 国立大学法人広島大学 | 骨軟骨修復を誘導する多能性幹細胞 |
KR20200070247A (ko) | 2017-10-17 | 2020-06-17 | 고쿠리츠다이가쿠호진 히로시마다이가쿠 | 골연골 수복을 유도하는 다능성 간세포 |
WO2020040135A1 (ja) | 2018-08-20 | 2020-02-27 | 剛士 田邊 | 細胞の培養又は誘導方法 |
US11898130B2 (en) | 2018-08-20 | 2024-02-13 | Peace, Inc. | Cell culture equipment |
WO2020040118A1 (ja) | 2018-08-20 | 2020-02-27 | アイ ピース, インコーポレイテッド | 細胞培養器 |
US12036245B2 (en) | 2018-11-07 | 2024-07-16 | I Peace, Inc. | Pharmaceutical composition and cosmetic composition |
WO2020179127A1 (ja) | 2019-03-05 | 2020-09-10 | ファナック株式会社 | 細胞製造システム |
WO2020250929A1 (ja) | 2019-06-10 | 2020-12-17 | アイ ピース, インコーポレイテッド | 赤血球除去装置、単核球回収器、細胞培養装置、細胞培養システム、細胞培養方法、及び単核球の回収方法 |
WO2020250927A1 (ja) | 2019-06-10 | 2020-12-17 | アイ ピース, インコーポレイテッド | 赤血球除去装置、単核球回収器、細胞培養装置、細胞培養システム、細胞培養方法、及び単核球の回収方法 |
WO2020262351A1 (ja) | 2019-06-28 | 2020-12-30 | アイ ピース, インコーポレイテッド | 細胞塊分割器、細胞塊分割器の製造方法、及び細胞塊の分割方法 |
WO2020262354A1 (ja) | 2019-06-28 | 2020-12-30 | アイ ピース, インコーポレイテッド | 細胞培養器及び細胞培養装置 |
WO2021038997A1 (ja) | 2019-08-29 | 2021-03-04 | ファナック株式会社 | 細胞製造装置 |
WO2021038996A1 (ja) | 2019-08-29 | 2021-03-04 | ファナック株式会社 | 細胞製造装置及びその製造方法 |
WO2021038998A1 (ja) | 2019-08-29 | 2021-03-04 | ファナック株式会社 | 細胞製造装置及びそのシステム |
EP4410947A2 (en) | 2019-08-29 | 2024-08-07 | Fanuc Corporation | Cell production device and system therefor |
WO2021085639A1 (ja) | 2019-10-31 | 2021-05-06 | 株式会社生命科学インスティテュート | 多能性幹細胞による間質性膀胱炎の治療 |
WO2021090767A1 (ja) | 2019-11-06 | 2021-05-14 | アイ ピース, インコーポレイテッド | 細胞培養装置 |
WO2021186648A1 (ja) | 2020-03-18 | 2021-09-23 | ファナック株式会社 | 顕微鏡観察システム |
WO2022050419A1 (ja) | 2020-09-04 | 2022-03-10 | Heartseed株式会社 | iPS細胞の品質改善剤、iPS細胞の製造方法、iPS細胞、及びiPS細胞製造用組成物 |
EP4019620A1 (en) | 2020-12-22 | 2022-06-29 | I Peace, Inc. | Cell culture vessel and method for culturing cell |
Also Published As
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP5943324B2 (ja) | 誘導多能性幹細胞 | |
US20220048963A1 (en) | Nuclear reprogramming factor and induced pluripotent stem cells | |
CA2695522C (en) | Method of nuclear reprogramming | |
US8278104B2 (en) | Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2 | |
US8129187B2 (en) | Somatic cell reprogramming by retroviral vectors encoding Oct3/4. Klf4, c-Myc and Sox2 | |
JP5626619B2 (ja) | 効率的な核初期化方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20080821 |
|
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20080902 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20110912 Year of fee payment: 3 |
|
R150 | Certificate of patent or registration of utility model |
Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20110912 Year of fee payment: 3 |
|
RD03 | Notification of appointment of power of attorney |
Free format text: JAPANESE INTERMEDIATE CODE: R3D03 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20270912 Year of fee payment: 19 |