SG10201810739VA - Use of small molecules to enhance mafa expression in pancreatic endocrine cells - Google Patents

Use of small molecules to enhance mafa expression in pancreatic endocrine cells

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Publication number
SG10201810739VA
SG10201810739VA SG10201810739VA SG10201810739VA SG10201810739VA SG 10201810739V A SG10201810739V A SG 10201810739VA SG 10201810739V A SG10201810739V A SG 10201810739VA SG 10201810739V A SG10201810739V A SG 10201810739VA SG 10201810739V A SG10201810739V A SG 10201810739VA
Authority
SG
Singapore
Prior art keywords
pancreatic endocrine
endocrine cells
small molecules
enhance
mafa expression
Prior art date
Application number
SG10201810739VA
Inventor
Alireza Rezania
Original Assignee
Janssen Biotech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Janssen Biotech Inc filed Critical Janssen Biotech Inc
Publication of SG10201810739VA publication Critical patent/SG10201810739VA/en

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/18—Drugs for disorders of the alimentary tract or the digestive system for pancreatic disorders, e.g. pancreatic enzymes
    • C—CHEMISTRY; METALLURGY
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    • C12N2500/00—Specific components of cell culture medium
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/22—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from pancreatic cells

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  • Health & Medical Sciences (AREA)
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  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
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  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

USE OF SMALL MOLECULES TO ENHANCE MAFA EXPRESSION IN PANCREATIC ENDOCRINE CELLS The present invention provides methods, cell cultures and differentiation media to promote differentiation of pluripotent stem cells to pancreatic endocrine cells of a mature phenotype. The resulting pancreatic endocrine cells express single hormonal insulin, PDX1, NKX6.1, and MAFA. In one or more differentiation stages, culturing may be carried out in a culture vessel at the air-liquid interface. No Suitable Figure
SG10201810739VA 2014-05-16 2015-05-07 Use of small molecules to enhance mafa expression in pancreatic endocrine cells SG10201810739VA (en)

Applications Claiming Priority (1)

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US201461994259P 2014-05-16 2014-05-16

Publications (1)

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SG10201810739VA true SG10201810739VA (en) 2019-01-30

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SG10201810739VA SG10201810739VA (en) 2014-05-16 2015-05-07 Use of small molecules to enhance mafa expression in pancreatic endocrine cells

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US (3) US10006006B2 (en)
EP (2) EP3143127B1 (en)
JP (1) JP6588969B2 (en)
KR (1) KR102162138B1 (en)
CN (2) CN106414720A (en)
AR (1) AR100446A1 (en)
AU (2) AU2015259573B2 (en)
BR (1) BR112016026626A2 (en)
CA (1) CA2949056A1 (en)
DK (1) DK3143127T3 (en)
MX (1) MX2016015004A (en)
PH (1) PH12016502274A1 (en)
RU (1) RU2694311C2 (en)
SG (2) SG11201609473XA (en)
SV (1) SV2016005319A (en)
WO (1) WO2015175307A1 (en)
ZA (1) ZA201608681B (en)

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