FI20096288A0 - Formulations and Methods for Culturing Stem Cells - Google Patents

Formulations and Methods for Culturing Stem Cells

Info

Publication number
FI20096288A0
FI20096288A0 FI20096288A FI20096288A FI20096288A0 FI 20096288 A0 FI20096288 A0 FI 20096288A0 FI 20096288 A FI20096288 A FI 20096288A FI 20096288 A FI20096288 A FI 20096288A FI 20096288 A0 FI20096288 A0 FI 20096288A0
Authority
FI
Finland
Prior art keywords
formulations
methods
stem cells
culturing stem
culturing
Prior art date
Application number
FI20096288A
Other languages
Finnish (fi)
Swedish (sv)
Inventor
Kristiina Rajala
Marjo-Riitta Suuronen
Outi Hovatta
Heli Skottman
Original Assignee
Kristiina Rajala
Marjo Riitta Suuronen
Outi Hovatta
Heli Skottman
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kristiina Rajala, Marjo Riitta Suuronen, Outi Hovatta, Heli Skottman filed Critical Kristiina Rajala
Priority to FI20096288A priority Critical patent/FI20096288A0/en
Publication of FI20096288A0 publication Critical patent/FI20096288A0/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues ; Not used, see subgroups
    • C12N5/0602Vertebrate cells
    • C12N5/0603Embryonic cells ; Embryoid bodies
    • C12N5/0606Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues ; Not used, see subgroups
    • C12N5/0602Vertebrate cells
    • C12N5/0652Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662Stem cells
    • C12N5/0667Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/05Inorganic components
    • C12N2500/10Metals; Metal chelators
    • C12N2500/20Transition metals
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/05Inorganic components
    • C12N2500/10Metals; Metal chelators
    • C12N2500/20Transition metals
    • C12N2500/24Iron; Fe chelators; Transferrin
    • C12N2500/25Insulin-transferrin; Insulin-transferrin-selenium
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/30Organic components
    • C12N2500/32Amino acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/30Organic components
    • C12N2500/36Lipids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/30Organic components
    • C12N2500/38Vitamins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/60Buffer, e.g. pH regulation, osmotic pressure
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/90Serum-free medium, which may still contain naturally-sourced components
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/98Xeno-free medium and culture conditions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/115Basic fibroblast growth factor (bFGF, FGF-2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/16Activin; Inhibin; Mullerian inhibiting substance
FI20096288A 2009-12-04 2009-12-04 Formulations and Methods for Culturing Stem Cells FI20096288A0 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
FI20096288A FI20096288A0 (en) 2009-12-04 2009-12-04 Formulations and Methods for Culturing Stem Cells

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
FI20096288A FI20096288A0 (en) 2009-12-04 2009-12-04 Formulations and Methods for Culturing Stem Cells
CA2782296A CA2782296A1 (en) 2009-12-04 2010-11-30 Formulations and methods for culturing stem cells
JP2012541546A JP2013512667A (en) 2009-12-04 2010-11-30 Formulations and methods for culturing stem cells
CN2010800545573A CN102906247A (en) 2009-12-04 2010-11-30 Formulations and methods for culturing stem cells
AU2010326512A AU2010326512A1 (en) 2009-12-04 2010-11-30 Formulations and methods for culturing stem cells
PCT/FI2010/050980 WO2011067465A1 (en) 2009-12-04 2010-11-30 Formulations and methods for culturing stem cells
EP10800970A EP2507360A1 (en) 2009-12-04 2010-11-30 Formulations and methods for culturing stem cells
KR1020127017428A KR20130009943A (en) 2009-12-04 2010-11-30 Formulations and methods for culturing stem cells

Publications (1)

Publication Number Publication Date
FI20096288A0 true FI20096288A0 (en) 2009-12-04

Family

ID=41462754

Family Applications (1)

Application Number Title Priority Date Filing Date
FI20096288A FI20096288A0 (en) 2009-12-04 2009-12-04 Formulations and Methods for Culturing Stem Cells

Country Status (8)

Country Link
EP (1) EP2507360A1 (en)
JP (1) JP2013512667A (en)
KR (1) KR20130009943A (en)
CN (1) CN102906247A (en)
AU (1) AU2010326512A1 (en)
CA (1) CA2782296A1 (en)
FI (1) FI20096288A0 (en)
WO (1) WO2011067465A1 (en)

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US9080145B2 (en) 2007-07-01 2015-07-14 Lifescan Corporation Single pluripotent stem cell culture
MX2010005805A (en) 2007-11-27 2010-06-09 Lifescan Inc Differentiation of human embryonic stem cells.
EP2254990A2 (en) 2008-02-21 2010-12-01 Centocor Ortho Biotech Inc. Methods, surface modified plates and compositions for cell attachment, cultivation and detachment
ES2552240T3 (en) 2008-06-30 2015-11-26 Janssen Biotech, Inc. Differentiation of pluripotent stem cells
ES2727950T3 (en) 2008-10-31 2019-10-21 Janssen Biotech Inc Differentiation of human embryonic stem cells in pancreatic endocrine lineage
WO2010051223A1 (en) 2008-10-31 2010-05-06 Centocor Ortho Biotech Inc. Differentiation of human embryonic stem cells to the pancreatic endocrine lineage
BRPI0921996A2 (en) 2008-11-20 2015-08-18 Centocor Ortho Biotech Inc Methods and compositions for culturing and binding cells on flat substrates.
MX2011005288A (en) 2008-11-20 2011-06-01 Centocor Ortho Biotech Inc Pluripotent stem cell culture on micro-carriers.
US10076544B2 (en) 2009-07-20 2018-09-18 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
KR101773538B1 (en) 2009-12-23 2017-08-31 얀센 바이오테크 인코포레이티드 Differentiation of human embryonic stem cells
US9969981B2 (en) 2010-03-01 2018-05-15 Janssen Biotech, Inc. Methods for purifying cells derived from pluripotent stem cells
CA2800610C (en) 2010-05-12 2019-09-24 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
WO2012030538A2 (en) 2010-08-31 2012-03-08 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
JP6218605B2 (en) 2010-08-31 2017-10-25 ヤンセン バイオテツク,インコーポレーテツド Differentiation of human embryonic stem cells
JP6148429B2 (en) * 2011-01-31 2017-06-14 協和発酵バイオ株式会社 Method for culturing human pluripotent stem cells
US9388386B2 (en) 2011-12-22 2016-07-12 Janssen Biotech, Inc. Differentiation of human embryonic stem cells into single hormonal insulin positive cells
CA2866590A1 (en) * 2012-03-07 2013-09-12 Janssen Biotech, Inc. Defined media for expansion and maintenance of pluripotent stem cells
RU2650813C2 (en) 2012-06-08 2018-04-17 Янссен Байотек, Инк. Use of epinephrine ligands to differentiate pancreatic endoderm cells
MX2015008577A (en) 2012-12-31 2015-09-07 Janssen Biotech Inc Culturing of human embryonic stem cells at the air-liquid interface for differentiation into pancreatic endocrine cells.
US10370644B2 (en) 2012-12-31 2019-08-06 Janssen Biotech, Inc. Method for making human pluripotent suspension cultures and cells derived therefrom
BR112015015701A2 (en) 2012-12-31 2017-07-11 Janssen Biotech Inc differentiation of human embryonic stem cells into endocrine pancreatic cells using hb9 regulators
KR20150103203A (en) 2012-12-31 2015-09-09 얀센 바이오테크 인코포레이티드 Suspension and clustering of human pluripotent cells for differentiation into pancreatic endocrine cells
CN105283541B (en) * 2013-05-30 2019-02-19 味之素株式会社 Culture medium is used in stem cell culture
CN103432108A (en) * 2013-07-16 2013-12-11 江苏大学 Uses of N-3 unsaturated fatty acid in preparation of hematopoietic stem cell mobilization promoters
AU2014364368A1 (en) * 2013-12-20 2016-07-07 Bio-Ess Laboratories, Llc Media for cell culture
WO2015175307A1 (en) 2014-05-16 2015-11-19 Janssen Biotech, Inc. Use of small molecules to enhance mafa expression in pancreatic endocrine cells
WO2016088243A1 (en) * 2014-12-05 2016-06-09 株式会社ニコン Determination device, observation system, observation method, program for same, method for manufacturing cell, and cell
JP2019506431A (en) * 2016-02-23 2019-03-07 ユニバーシティ−インダストリー コーオペレイション グループ オブ キョンヒ ユニバーシティUniversity−Industry Cooperation Group Of Kyung Hee University Compositions and methods for improving efficacy of stem cells
CN109563478A (en) 2016-04-14 2019-04-02 詹森生物科技公司 Pluripotent stem cell differentiation is at gut entoderm cell in intestines
KR101896803B1 (en) * 2016-12-12 2018-09-07 서울대학교병원 Method for increasing the rate of inducing differentiation of human pluripotent stem cells to mesenchymal stem cells, and mesenchymal stem cells produced by thereof
AU2018209127A1 (en) * 2017-01-23 2019-08-08 Stemcell Technologies Canada Inc. Media and methods for enhancing the survival and proliferation of stem cells
WO2019072889A1 (en) * 2017-10-13 2019-04-18 Boehringer Ingelheim International Gmbh Perfusion medium

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US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
JP2001508302A (en) 1997-01-10 2001-06-26 ライフ テクノロジーズ,インコーポレイテッド Embryonic stem cell serum replacement
US6090622A (en) 1997-03-31 2000-07-18 The Johns Hopkins School Of Medicine Human embryonic pluripotent germ cells
WO2003018780A1 (en) 2001-08-27 2003-03-06 Advanced Cell Technology, Inc. De-differentiation and re-differentiation of somatic cells and production of cells for cell therapies
CN101052712A (en) * 2004-09-08 2007-10-10 威斯康星校友研究基金会 Culturing human embryonic stem cells
DE102005054577A1 (en) * 2005-11-16 2007-05-24 Cognis Ip Management Gmbh Use of esters of unsaturated, physiologically active fatty acids as nutrient media for cell cultures
TW200801513A (en) 2006-06-29 2008-01-01 Fermiscan Australia Pty Ltd Improved process
GB0622394D0 (en) * 2006-11-09 2006-12-20 Univ Cambridge Tech Differentiation of pluripotent cells
FI20075417A0 (en) * 2007-06-05 2007-06-05 Marjo-Riitta Suuronen Formulations and methods for culturing embryonic stem cells

Also Published As

Publication number Publication date
WO2011067465A1 (en) 2011-06-09
JP2013512667A (en) 2013-04-18
CN102906247A (en) 2013-01-30
AU2010326512A1 (en) 2012-06-28
FI20096288D0 (en)
CA2782296A1 (en) 2011-06-09
EP2507360A1 (en) 2012-10-10
KR20130009943A (en) 2013-01-24

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