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Definitions

• RNAi refers to antisense-mediated gene silencing through a mechanism that utilizes the RNA- induced siliencing complex (RISC).
• RNA target function is by an occupancy-based mechanism such as is employed naturally by microRNA.
• MicroRNAs are small non-coding RNAs that regulate the expression of protein- coding RNAs. The binding of an antisense compound to a microRNA prevents that microRNA from binding to its messenger RNA targets, and thus interferes with the function of the microRNA. MicroRNA mimics can enhance native microRNA function. Certain antisense compounds alter splicing of pre-mRNA. Regardless of the specific mechanism, sequence-specificity makes antisense compounds attractive as tools for target validation and gene functionalization, as well as therapeutics to selectively modulate the expression of genes involved in the pathogenesis of diseases.
• Antisense technology is an effective means for modulating the expression of one or more specific gene products and can therefore prove to be uniquely useful in a number of therapeutic, diagnostic, and research applications.
• Chemically modified nucleosides may be incorporated into antisense compounds to enhance one or more properties, such as nuclease resistance, pharmacokinetics or affinity for a target nucleic acid.
• Vitravene® flamivirsen; developed by Isis Pharmaceuticals Inc., Carlsbad, CA
• FDA U.S. Food and Drug Administration
• CMV cytomegalovirus
• New chemical modifications have improved the potency and efficacy of antisense compounds, uncovering the potential for oral delivery as well as enhancing subcutaneous administration, decreasing potential for side effects, and leading to improvements in patient convenience.
• Chemical modifications increasing potency of antisense compounds allow administration of lower doses, which reduces the potential for toxicity, as well as decreasing overall cost of therapy. Modifications increasing the resistance to degradation result in slower clearance from the body, allowing for less frequent dosing.
• Different types of chemical modifications can be combined in one compound to further optimize the compound's efficacy.
• the present disclosure provides conjugated antisense compounds. In certain embodiments, the present disclosure provides conjugated antisense compounds comprising an antisense oligonucleotide complementary to a nucleic acid transcript. In certain embodiments, the present disclosure provides methods comprising contacting a cell with a conjugated antisense compound comprising an antisense oligonucleotide complementary to a nucleic acid transcript. In certain embodiments, the present disclosure provides methods comprising contacting a cell with a conjugated antisense compound comprising an antisense oligonucleotide and reducing the amount or activity of a nucleic acid transcript in a cell.
• ASGP-R asialoglycoprotein receptor
• GalNAc N- acetylgalactosamine
• GalNAc-containing conjugates increase activity of duplex siRNA compounds in liver cells in vivo.
• the GalNAc-containing conjugate is typically attached to the sense strand of the siRNA duplex. Since the sense strand is discarded before the antisense strand ultimately hybridizes with the target nucleic acid, there is little concern that the conjugate will interfere with activity.
• the conjugate is attached to the 3' end of the sense strand of the siRNA. See e.g., U.S. Patent 8,106,022.
• Certain conjugate groups described herein are more active and/or easier to synthesize than conjugate groups previously described.
• conjugates are attached to single-stranded antisense compounds, including, but not limited to RNase H based antisense compounds and antisense compounds that alter splicing of a pre-mRNA target nucleic acid.
• the conjugate should remain attached to the antisense compound long enough to provide benefit (improved uptake into cells) but then should either be cleaved, or otherwise not interfere with the subsequent steps necessary for activity, such as hybridization to a target nucleic acid and interaction with RNase H or enzymes associated with splicing or splice modulation.
• This balance of properties is more important in the setting of single-stranded antisense compounds than in siRNA compounds, where the conjugate may simply be attached to the sense strand.
• conjugated single-stranded antisense compounds having improved potency in liver cells in vivo compared with the same antisense compound lacking the conjugate. Given the required balance of properties for these compounds such improved potency is surprising.
• conjugate groups herein comprise a cleavable moiety.
• the conjugate should remain on the compound long enough to provide enhancement in uptake, but after that, it is desirable for some portion or, ideally, all of the conjugate to be cleaved, releasing the parent compound (e.g., antisense compound) in its most active form.
• the cleavable moiety is a cleavable nucleoside.
• Such embodiments take advantage of endogenous nucleases in the cell by attaching the rest of the conjugate (the cluster) to the antisense oligonucleotide through a nucleoside via one or more cleavable bonds, such as those of a phosphodiester linkage.
• the cluster is bound to the cleavable nucleoside through a phosphodiester linkage.
• the cleavable nucleoside is attached to the antisense oligonucleotide (antisense compound) by a phosphodiester linkage.
• the conjugate group may comprise two or three cleavable nucleosides.
• such cleavable nucleosides are linked to one another, to the antisense compound and or to the cluster via cleavable bonds (such as those of a phosphodiester linkage).
• Certain conjugates herein do not comprise a cleavable nucleoside and instead comprise a cleavable bond. It is shown that that sufficient cleavage of the conjugate from the oligonucleotide is provided by at least one bond that is vulnerable to cleavage in the cell (a cleavable bond).
• conjugated antisense compounds are prodrugs. Such prodrugs are administered to an animal and are ultimately metabolized to a more active form. For example, conjugated antisense compounds are cleaved to remove all or part of the conjugate resulting in the active (or more active) form of the antisense compound lacking all or some of the conjugate.
• conjugates are attached at the 5' end of an oligonucleotide. Certain such 5'- conjugates are cleaved more efficiently than counterparts having a similar conjugate group attached at the 3' end. In certain embodiments, improved activity may correlate with improved cleavage. In certain embodiments, oligonucleotides comprising a conjugate at the 5' end have greater efficacy than oligonucleotides comprising a conjugate at the 3' end (see, for example, Examples 56, 81, 83, and 84). Further, 5'-attachment allows simpler oligonucleotide synthesis. Typically, oligonucleotides are synthesized on a solid support in the 3' to 5' direction.
• oligonucleotide typically one attaches a pre-conjugated 3' nucleoside to the solid support and then builds the oligonucleotide as usual.
• attaching that conjugated nucleoside to the solid support adds complication to the synthesis.
• the conjugate is then present throughout the synthesis of the oligonucleotide and can become degraded during subsequent steps or may limit the sorts of reactions and reagents that can be used.
• conjugates and conjugated oligonucleotides are easier and/or requires few steps, and is therefore less expensive than that of conjugates previously disclosed, providing advantages in manufacturing.
• the synthesis of certain conjugate groups consists of fewer synthetic steps, resulting in increased yield, relative to conjugate groups previously described.
• Conjugate groups such as GalNAc3-10 in Example 46 and GalNAc3-7 in Example 48 are much simpler than previously described conjugates such as those described in U.S. 8,106,022 or U.S. 7,262,177 that require assembly of more chemical intermediates .
• conjugate groups having only one or two GalNAc ligands improve activity of antisense compounds. Such compounds are much easier to prepare than conjugates comprising three GalNAc ligands.
• Conjugate groups comprising one or two GalNAc ligands may be attached to any antisense compounds, including single-stranded oligonucleotides and either strand of double-stranded oligonucleotides (e.g., siRNA).
• the conjugates herein do not substantially alter certain measures of tolerability.
• conjugated antisense compounds are not more immunogenic than unconjugated parent compounds. Since potency is improved, embodiments in which tolerability remains the same (or indeed even if tolerability worsens only slightly compared to the gains in potency) have improved properties for therapy.
• conjugation allows one to alter antisense compounds in ways that have less attractive consequences in the absence of conjugation. For example, in certain embodiments, replacing one or more phosphorothioate linkages of a fully phosphorothioate antisense compound with phosphodiester linkages results in improvement in some measures of tolerability. For example, in certain instances, such antisense compounds having one or more phosphodiester are less immunogenic than the same compound in which each linkage is a phosphorothioate. However, in certain instances, as shown in Example 26, that same replacement of one or more phosphorothioate linkages with phosphodiester linkages also results in reduced cellular uptake and/or loss in potency.
• conjugated antisense compounds described herein tolerate such change in linkages with little or no loss in uptake and potency when compared to the conjugated full-phosphorothioate counterpart.
• oligonucleotides comprising a conjugate and at least one phosphodiester internucleoside linkage actually exhibit increased potency in vivo even relative to a full phosphorothioate counterpart also comprising the same conjugate.
• conjugated antisense compounds comprise at least one phosphodiester linkage.
• conjugation of antisense compounds herein results in increased delivery, uptake and activity in hepatocytes.
• more compound is delivered to liver tissue.
• that increased delivery alone does not explain the entire increase in activity.
• more compound enters hepatocytes.
• even that increased hepatocyte uptake does not explain the entire increase in activity.
• productive uptake of the conjugated compound is increased.
• certain embodiments of GalNAc-containing conjugates increase enrichment of antisense oligonucleotides in hepatocytes versus non- parenchymal cells. This enrichment is beneficial for oligonucleotides that target genes that are expressed in hepatocytes.
• conjugated antisense compounds herein result in reduced kidney exposure.
• concentrations of antisense oligonucleotides comprising certain embodiments of GalNAc-containing conjugates are lower in the kidney than that of antisense oligonucleotides lacking a GalNAc-containing conjugate.
• This has several beneficial therapeutic implications. For therapeutic indications where activity in the kidney is not sought, exposure to kidney risks kidney toxicity without corresponding benefit.
• high concentration in kidney typically results in loss of compound to the urine resulting in faster clearance. Accordingly for non-kidney targets, kidney accumulation is undesired.
• conjugated antisense compounds represented by the formula:
• A is the antisense oligonucleotide
• each E is a tether
• each F is a ligand
• q is an integer between 1 and 5.
• conjugated antisense compounds having the structure:
• conjugated antisense compounds having the structure:
• conjugated antisense compounds having the structure:
• conjugated antisense compounds having the structure:
• Embodiment 1 The conjugated antisense compound of any of embodiments 1179 to 1182, wherein the tether has a structure selected from among: wherein each n is independent by, 0, 1, 2, 3, 4, 5, 6, or 7.
• Embodiment 2 The conjugated antisense compound of any of embodiments 1179 to 1182, wherein the tether has the structure:
• Embodiment 3 The conjugated antisense compound of any of embodiments 1179 to 1182 or 1688 to
• linker has a structure selected from among:
• Embodiment 4 The conjugated antisense compound of any of embodiments 1179 to 1182 or 1688 to 1689, wherein the linker has a structure selected from among:
• n is independently, 0, 1, 2, 3, 4, 5, 6, or 7.
• Embodiment 5 The conjugated antisense compound of any of embodiments 1179 to 1182 or 1688 to 1689, wherein the linker has the structure:
• each such particular variable is selected independently.
• each n is selected inde endently, so they may or may not be the same as one another.
• 2'-F nucleoside refers to a nucleoside comprising a sugar comprising fluorine at the 2' position. Unless otherwise indicated, the fluorine in a 2'-F nucleoside is in the ribo position (replacing the OH of a natural ribose).
• 2'-0-methoxyethyl refers to an O-methoxy-ethyl modification of the 2' position of a furosyl ring.
• a 2'-0-methoxyethyl modified sugar is a modified sugar.
• 2'-0-methoxyethyl nucleotide means a nucleotide comprising a 2'-0-methoxyethyl modified sugar moiety.
• 2 '-substituted sugar moiety means a furanosyl comprising a substituent at the 2'- position other than H or OH. Unless otherwise indicated, a 2'-substituted sugar moiety is not a bicyclic sugar moiety (i.e., the 2'-substituent of a 2 '-substituted sugar moiety does not form a bridge to another atom of the furanosyl ring.
• 3' target site refers to the nucleotide of a target nucleic acid which is complementary to the 3'-most nucleotide of a particular antisense compound.
• 5' target site refers to the nucleotide of a target nucleic acid which is complementary to the 5'-most nucleotide of a particular antisense compound.
• 5-methylcytosine means a cytosine modified with a methyl group attached to the 5' position.
• a 5- methylcytosine is a modified nucleobase.
• “About” means within ⁇ 10% of a value. For example, if it is stated, “a marker may be increased by about 50%", it is implied that the marker may be increased between 45%-55%.
• Active pharmaceutical agent means the substance or substances in a pharmaceutical composition that provide a therapeutic benefit when administered to an individual. For example, in certain embodiments an antisense oligonucleotide targeted to PTP1B is an active pharmaceutical agent.
• Active target region or “target region” means a region to which one or more active antisense compounds is targeted.
• Active antisense compounds means antisense compounds that reduce target nucleic acid levels or protein levels.
• Adipogenesis means the development of fat cells from preadipocytes.
• Lipogenesis means the production or formation of fat, either fatty degeneration or fatty infiltration.
• Body fat distribution can be estimated by skin-fold measures, waist-to-hip circumference ratios, or techniques such as ultrasound, computed tomography, or magnetic resonance imaging. According to the Center for Disease Control and Prevention, individuals with a body mass index (BMI) of 30 or more are considered obese.
• BMI body mass index
• the term "obesity” includes, but is not limited to, the following conditions: adult-onset obesity; alimentary obesity; endogenous or inflammatory obesity; endocrine obesity; familial obesity; hyperinsulinar obesity; hype ⁇ lastic-hypertrophic obesity; hypogonadal obesity; hypothyroid obesity; lifelong obesity; morbid obesity and exogenous obesity.
• "Administered concomitantly” refers to the co-administration of two agents in any manner in which the pharmacological effects of both are manifest in the patient at the same time. Concomitant administration does not require that both agents be administered in a single pharmaceutical composition, in the same dosage form, or by the same route of administration. The effects of both agents need not manifest themselves at the same time. The effects need only be overlapping for a period of time and need not be coextensive.
• administering means providing an agent to an animal, and includes, but is not limited to, administering by a medical professional and self-administering.
• Agent means an active substance that can provide a therapeutic benefit when administered to an animal.
• First Agent means a therapeutic compound provided herein.
• a first agent can be an antisense oligonucleotide targeting PTP1B.
• second agent means a second therapeutic compound of the invention (e.g. a second antisense oligonucleotide targeting PTP1B) and/or a non- PTP1B therapeutic compound.
• “Amelioration” refers to a lessening of at least one indicator, sign, or symptom of an associated disease, disorder, or condition. The severity of indicators can be determined by subjective or objective measures, which are known to those skilled in the art.
• “Animal” refers to a human or non-human animal, including, but not limited to, mice, rats, rabbits, dogs, cats, pigs, and non-human primates, including, but not limited to, monkeys and chimpanzees.
• Antisense activity means any detectable or measurable activity attributable to the hybridization of an antisense compound to its target nucleic acid. In certain embodiments, antisense activity is a decrease in the amount or expression of a target nucleic acid or protein encoded by such target nucleic acid.
• Antisense compound means an oligomeric compound that is capable of undergoing hybridization to a target nucleic acid through hydrogen bonding.
• Antisense inhibition means reduction of target nucleic acid levels or target protein levels in the presence of an antisense compound complementary to a target nucleic acid compared to target nucleic acid levels or target protein levels in the absence of the antisense compound.
• Antisense oligonucleotide means a single-stranded oligonucleotide having a nucleobase sequence that permits hybridization to a corresponding region or segment of a target nucleic acid.
• Bicyclic sugar means a furosyl ring modified by the bridging of two non-geminal ring atoms.
• a bicyclic sugar is a modified sugar.
• BNA Bicyclic nucleic acid
• BNA a nucleoside or nucleotide wherein the furanose portion of the nucleoside or nucleotide includes a bridge connecting two carbon atoms on the furanose ring, thereby forming a bicyclic ring system
• bicyclic sugar moiety means a modified sugar moiety comprising a 4 to 7 membered ring (including but not limited to a furanosyl) comprising a bridge connecting two atoms of the 4 to 7 membered ring to form a second ring, resulting in a bicyclic structure.
• the 4 to 7 membered ring is a sugar ring.
• the 4 to 7 membered ring is a furanosyl.
• the bridge connects the 2'-carbon and the 4'-carbon of the furanosyl.
• Cap structure or "terminal cap moiety” means chemical modifications, which have been incorporated at either terminus of an antisense compound.
• “Chemically distinct region” refers to a region of an antisense compound that is in some way chemically different than another region of the same antisense compound. For example, a region having 2'- O-methoxyethyl nucleotides is chemically distinct from a region having nucleotides without 2'-0- methoxyethyl modifications.
• chemical modification means a chemical difference in a compound when compared to a naturally occurring counterpart.
• Chemical modifications of oligonucleotides include nucleoside modifications (including sugar moiety modifications and nucleobase modifications) and internucleoside linkage modifications. In reference to an oligonucleotide, chemical modification does not include differences only in nucleobase sequence.
• Chimeric antisense compound means an antisense compound that has at least two chemically distinct regions.
• Co-administration means administration of two or more agents to an individual.
• the two or more agents can be in a single pharmaceutical composition, or can be in separate pharmaceutical compositions.
• Each of the two or more agents can be administered through the same or different routes of administration.
• Co-administration encompasses parallel or sequential administration.
• Cholesterol is a sterol molecule found in the cell membranes of all animal tissues. Cholesterol must be transported in an animal's blood plasma by lipoproteins including very low density lipoprotein (VLDL), intermediate density lipoprotein (IDL), low density lipoprotein (LDL), and high density lipoprotein (HDL).
• VLDL very low density lipoprotein
• IDL intermediate density lipoprotein
• LDL low density lipoprotein
• HDL high density lipoprotein
• Plasma cholesterol refers to the sum of all lipoproteins (VOL, IDL, LDL, HDL) esterified and/or non- esterified cholesterol present in the plasma or serum.
• “Cholesterol absorption inhibitor” means an agent that inhibits the absorption of exogenous cholesterol obtained from diet.
• “Complementarity” means the capacity for pairing between nucleobases of a first nucleic acid and a second nucleic acid.
• constrained ethyl nucleoside or “cEt” means a nucleoside comprising a bicyclic sugar moiety comprising a 4'-CH(CH 3 )-0-2'bridge.
• Contiguous nucleobases means nucleobases immediately adjacent to each other.
• Deoxyribonucleotide means a nucleotide having a hydrogen at the 2' position of the sugar portion of the nucleotide. Deoxyribonucleotides may be modified with any of a variety of substituents.
• Diabetes mellitus or "diabetes” is a syndrome characterized by disordered metabolism and abnormally high blood sugar (hyperglycemia) resulting from insufficient levels of insulin or reduced insulin sensitivity.
• the characteristic symptoms are excessive urine production (polyuria) due to high blood glucose levels, excessive thirst and increased fluid intake (polydipsia) attempting to compensate for increased urination, blurred vision due to high blood glucose effects on the eye's optics, unexplained weight loss, and lethargy.
• Diabetic dyslipidemia or "type 2 diabetes with dyslipidemia” means a condition characterized by Type 2 diabetes, reduced HDL-C, elevated triglycerides, and elevated small, dense LDL particles.
• “Diluent” means an ingredient in a composition that lacks pharmacological activity, but is pharmaceutically necessary or desirable.
• the diluent in an injected composition can be a liquid, e.g. saline solution.
• “Dyslipidemia” refers to a disorder of lipid and/or lipoprotein metabolism, including lipid and/or lipoprotein overproduction or deficiency. Dyslipidemias may be manifested by elevation of lipids such as cholesterol and triglycerides as well as lipoproteins such as low-density lipoprotein (LDL) cholesterol.
• LDL low-density lipoprotein
• Dosage unit means a form in which a pharmaceutical agent is provided, e.g. pill, tablet, or other dosage unit known in the art.
• a dosage unit is a vial containing lyophihzed antisense oligonucleotide.
• a dosage unit is a vial containing reconstituted antisense oligonucleotide.
• Dose means a specified quantity of a pharmaceutical agent provided in a single administration, or in a specified time period.
• a dose can be administered in one, two, or more boluses, tablets, or injections.
• the desired dose requires a volume not easily accommodated by a single injection, therefore, two or more injections can be used to achieve the desired dose.
• the pharmaceutical agent is administered by infusion over an extended period of time or continuously. Doses can be stated as the amount of pharmaceutical agent per hour, day, week, or month.
• Effective amount or “therapeutically effective amount” means the amount of active
• the effective amount can vary among individuals depending on the health and physical condition of the individual to be treated, the taxonomic group of the individuals to be treated, the formulation of the composition, assessment of the individual's medical condition, and other relevant factors.
• “Fully complementary” or “100% complementary” means each nucleobase of a nucleobase sequence of a first nucleic acid has a complementary nucleobase in a second nucleobase sequence of a second nucleic acid.
• a first nucleic acid is an antisense compound and a target nucleic acid is a second nucleic acid.
• furanosyl means a structure comprising a 5-membered ring comprising four carbon atoms and one oxygen atom.
• Gapmer means a chimeric antisense compound in which an internal region having a plurality of nucleosides that support RNase H cleavage is positioned between external regions having one or more nucleosides, wherein the nucleosides comprising the internal region are chemically distinct from the nucleoside or nucleosides comprising the external regions.
• the internal region can be referred to as a "gap segment” and the external regions can be referred to as "wing segments.”
• Gap-widened means a chimeric antisense compound having a gap segment of 12 or more contiguous 2'-deoxyribonucleosides positioned between and immediately adjacent to 5' and 3' wing segments having from one to six nucleosides.
• Glucose is a monosaccharide used by cells as a source of energy and inflammatory intermediate.
• Plasma glucose refers to glucose present in the plasma.
• HMG-CoA reductase inhibitor means an agent that acts through the inhibition of the enzyme
• HMG-CoA reductase such as atorvastatin, rosuvastatin, fluvastatin, lovastatin, pravastatin, and simvastatin.
• Hybridization means the annealing of complementary nucleic acid molecules.
• complementary nucleic acid molecules include an antisense compound and a target nucleic acid.
• “Hyperlipidemia” or “hyperlipemia” is a condition characterized by elevated serum lipids or circulating (plasma) lipids. This condition manifests an abnormally high concentration of fats.
• the lipid fractions in the circulating blood are cholesterol, low density lipoproteins, very low density lipoproteins and triglycerides.
• “Hypertriglyceridemia” means a condition characterized by elevated triglyceride levels.
• Identifying or “selecting an animal with metabolic” means identifying or selecting a subject having been diagnosed with a metabolic disease, or a metabolic disorder; or, identifying or selecting a subject having any symptom of a metabolic disease, including, but not limited to, metabolic syndrome, hyperglycemia, hypertriglyceridemia, hypertension increased insulin resistance, decreased insulin sensitivity, above normal body weight, and/or above normal body fat or any combination thereof.
• identification may be accomplished by any method, including but not limited to, standard clinical tests or assessments, such as measuring serum or circulating (plasma) blood-glucose, measuring serum or circulating (plasma) triglycerides, measuring blood-pressure, measuring body fat, measuring body weight, and the like.
• “Individual” or “subject” or “animal” means a human or non-human animal selected for treatment or therapy.
• Insulin resistance is defined as the condition in which normal amounts of insulin are inadequate to produce a normal insulin response from fat, muscle and liver cells. Insulin resistance in fat cells results in hydrolysis of stored triglycerides, which elevates free fatty acids in the blood plasma. Insulin resistance in muscle reduces glucose uptake whereas insulin resistance in liver reduces glucose storage, with both effects serving to elevate blood glucose. High plasma levels of insulin and glucose due to insulin resistance often leads to metabolic syndrome and type 2 diabetes.
• Insulin sensitivity is a measure of how effectively an individual processes glucose. An individual having high insulin sensitivity effectively processes glucose whereas an individual with low insulin sensitivity does not effectively process glucose.
• Internucleoside linkage refers to the chemical bond between nucleosides.
• Intravenous administration means administration into a vein.
• Linked nucleosides means adjacent nucleosides which are bonded together.
• locked nucleic acid nucleoside or "LNA” means a nucleoside comprising a bicyclic sugar moiety comprising a 4'-CH2-0-2'bridge.
• lipid-lowering therapy or "lipid lowering agent” means a therapeutic regimen provided to a subject to reduce one or more lipids in a subject.
• a lipid-lowering therapy is provided to reduce one or more of ApoB, total cholesterol, LDL-C, VLDL-C, IDL-C, non-HDL-C, triglycerides, small dense LDL particles, and Lp(a) in a subject.
• lipid-lowering therapy include statins, fibrates, and MTP inhibitors.
• Major risk factors refers to factors that contribute to a high risk for a particular disease or condition.
• major risk factors for coronary heart disease include, without limitation, cigarette smoking, hypertension, low HDL-C, family history of coronary heart disease, age, and other factors disclosed herein.
• Metabolic disease or “metabolic disorder” refers to a condition characterized by an alteration or disturbance in metabolic function.
• Metabolic and “metabolism” are terms well known in the art and generally include the whole range of biochemical processes that occur within a living organism.
• Metabolic diseases or disorders include, but are not limited to, obesity, diabetes, hyperglycemia, prediabetes, nonalcoholic fatty liver disease (NAFLD), metabolic syndrome, insulin resistance, diabetic dyslipidemia, or hypertriglyceridemia or a combination thereof.
• NAFLD nonalcoholic fatty liver disease
• Metabolic syndrome means a condition characterized by a clustering of lipid and non- lipid cardiovascular risk factors of metabolic origin.
• metabolic syndrome is identified by the presence of any 3 of the following factors: waist circumference of greater than 102 cm in men or greater than 88 cm in women; serum triglyceride of at least 150 mg/dL; HDL-C less than 40 mg/dL in men or less than 50 mg/dL in women; blood pressure of at least 130/85 mmHg; and fasting glucose of at least 110 mg/dL.
• mismatch or “non-complementary nucleobase” refers to the case when a nucleobase of a first nucleic acid is not capable of pairing with the corresponding nucleobase of a second or target nucleic acid.
• Mated dyslipidemia means a condition characterized by elevated cholesterol and elevated triglycerides.
• Modified internucleoside linkage refers to a substitution or any change from a naturally occurring internucleoside bond (i.e. a phosphodiester internucleoside bond).
• Modified nucleobase refers to any nucleobase other than adenine, cytosine, guanine, thymidine, or uracil.
• An "unmodified nucleobase” means the purine bases adenine (A) and guanine (G), and the pyrimidine bases thymine (T), cytosine (C), and uracil (U).
• Modified nucleoside means a nucleoside having, independently, a modified sugar moiety or modified nucleobase.
• Modified nucleotide means a nucleotide having, independently, a modified sugar moiety, modified internucleoside linkage, or modified nucleobase.
• a “modified nucleoside” means a nucleoside having, independently, a modified sugar moiety or modified nucleobase.
• Modified oligonucleotide means an oligonucleotide comprising at least one modified nucleotide.
• Modified sugar refers to a substitution or change from a natural sugar.
• modified sugar moiety means a substituted sugar moiety or a sugar surrogate.
• MOE means -OCH 2 CH 2 OCH 3 .
• Microtif means the pattern of chemically distinct regions in an antisense compound.
• MTP inhibitor means an agent inhibits the enzyme, microsomal triglyceride transfer protein.
• Naturally occurring internucleoside linkage means a 3' to 5' phosphodiester linkage.
• naturally occurring sugar moiety means a ribofuranosyl as found in naturally occurring RNA or a deoxyribofuranosyl as found in naturally occurring DNA.
• Natural sugar moiety means a sugar found in DNA (2'-H) or RNA (2'-OH).
• Non-alcoholic fatty liver disease or “NAFLD” means a condition characterized by fatty inflammation of the liver that is not due to excessive alcohol use (for example, alcohol consumption of over 20 g day).
• NAFLD is related to insulin resistance and the metabolic syndrome.
• NAFLD encompasses a disease spectrum ranging from simple triglyceride accumulation in hepatocytes (hepatic steatosis) to hepatic steatosis with inflammation (steatohepatitis), fibrosis, and cirrhosis.
• NASH Nonalcoholic steatohepatitis
• a “second hit” capable of inducing necrosis, inflammation, and fibrosis is required for development of NASH.
• Candidates for the second-hit can be grouped into broad categories: factors causing an increase in oxidative stress and factors promoting expression of proinflammatory cytokines
• Nucleic acid refers to molecules composed of monomeric nucleotides.
• a nucleic acid includes ribonucleic acids (RNA), deoxyribonucleic acids (DNA), single-stranded nucleic acids, double-stranded nucleic acids, small interfering ribonucleic acids (siRNA), and microRNAs (miRNA).
• RNA ribonucleic acids
• DNA deoxyribonucleic acids
• siRNA small interfering ribonucleic acids
• miRNA microRNAs
• Nucleobase means a heterocyclic moiety capable of pairing with a base of another nucleic acid.
• nucleobase means a group of atoms that can be linked to a sugar moiety to create a nucleoside that is capable of incorporation into an oligonucleotide, and wherein the group of atoms is capable of bonding with a complementary naturally occurring nucleobase of another oligonucleotide or nucleic acid.
• Nucleobases may be naturally occurring or may be modified.
• Nucleobase sequence means the order of contiguous nucleobases independent of any sugar, linkage, or nucleobase modification.
• nucleoside means a compound comprising a nucleobase moiety and a sugar moiety. Nucleosides include, but are not limited to, naturally occurring nucleosides (as found in DNA and RNA) and modified nucleosides. Nucleosides may be linked to a phosphate moiety.
• Nucleoside mimetic includes those structures used to replace the sugar or the sugar and the base and not necessarily the linkage at one or more positions of an oligomeric compound such as for example nucleoside mimetics having morpholino, cyclohexenyl, cyclohexyl, tetrahydropyranyl, bicyclo or tricyclo sugar mimetics e.g. non furanose sugar units.
• Nucleotide means a nucleoside having a phosphate group covalently linked to the sugar portion of the nucleoside.
• nucleotide means a nucleoside further comprising a phosphate linking group.
• linked nucleosides may or may not be linked by phosphate linkages and thus includes, but is not limited to “linked nucleotides.”
• linked nucleosides are nucleosides that are connected in a continuous sequence (i.e. no additional nucleosides are present between those that are linked).
• Oligomeric compound refers to a polymeric structure comprising two or more substructures and capable of hybridizing to a region of a nucleic acid molecule.
• oligomeric compounds are oligonucleosides. In certain embodiments, oligomeric compounds are
• oligomeric compounds are antisense compounds. In certain embodiments, oligomeric compounds are antisense oligonucleotides. In certain embodiments, oligomeric compounds are chimeric oligonucleotides.
• Oligonucleotide means a polymer of linked nucleosides each of which can be modified or unmodified, independent one from another.
• Parenteral administration means administration through injection or infusion. Parenteral administration includes subcutaneous administration, intravenous administration, intramuscular
• Administration can be continuous, or chronic, or short or intermittent.
• Peptide means a molecule formed by linking at least two amino acids by amide bonds. Peptide refers to polypeptides and proteins.
• “Pharmaceutical agent” means a substance that provides a therapeutic benefit when administered to an individual. For example, in certain embodiments, an antisense oligonucleotide targeted to PTPIB is pharmaceutical agent.
• “Pharmaceutical composition” means a mixture of substances suitable for administering to an individual.
• a pharmaceutical composition can comprise one or more active agents and a sterile aqueous solution.
• “Pharmaceutically acceptable carrier” means a medium or diluent that does not interfere with the structure of the oligonucleotide. Certain, of such carries enable pharmaceutical compositions to be formulated as, for example, tablets, pills, dragees, capsules, liquids, gels, syrups, slurries, suspension and lozenges for the oral ingestion by a subject.
• a pharmaceutically acceptable carrier can be a sterile aqueous solution.
• “Pharmaceutically acceptable derivative” encompasses pharmaceutically acceptable salts, conjugates, prodrugs or isomers of the compounds described herein.
• “Pharmaceutically acceptable salts” means physiologically and pharmaceutically acceptable salts of antisense compounds, i.e., salts that retain the desired biological activity of the parent oligonucleotide and do not impart undesired toxicological effects thereto.
• Phosphorothioate linkage means a linkage between nucleosides where the phosphodiester bond is modified by replacing one of the non-bridging oxygen atoms with a sulfur atom.
• a phosphorothioate linkage is a modified internucleoside linkage.
• Portion means a defined number of contiguous (i.e. linked) nucleobases of a nucleic acid. In certain embodiments, a portion is a defined number of contiguous nucleobases of a target nucleic acid. In certain embodiments, a portion is a defined number of contiguous nucleobases of an antisense compound.
• Prevent refers to delaying or forestalling the onset or development of a disease, disorder, or condition for a period of time from minutes to indefinitely. Prevent also means reducing risk of developing a disease, disorder, or condition.
• Prodrug means a therapeutic agent that is prepared in an inactive form that is converted to an active form within the body or cells thereof by the action of endogenous enzymes or other chemicals or conditions.
• Protein tyrosine phosphatase IB or "PTPIB” (also known as PTPN1 ; protein tyrosine phosphatase, non-receptor type 1 ; PTP-1B; RKPTP) means any nucleic acid or protein of PTPIB.
• PTPIB expression means the level of mRNA transcribed from the gene encoding PTPIB or the level of protein translated from the mRNA. PTPIB expression can be determined by art known methods such as a Northern or Western blot.
• PTPIB nucleic acid means any nucleic acid encoding PTPIB. For example, in certain embodiments
• a PTPIB nucleic acid includes a DNA sequence encoding PTPIB, a RNA sequence transcribed from DNA encoding PTP1B (including genomic DNA comprising introns and exons), and a mPvNA sequence encoding PTP1B.
• PTP1B mRNA means a m NA encoding a PTP1B protein.
• Side effects means physiological responses attributable to a treatment other than the desired effects.
• side effects include injection site reactions, liver function test abnormalities, renal function abnormalities, liver toxicity, renal toxicity, central nervous system abnormalities, myopathies, and malaise.
• increased aminotransferase levels in serum can indicate liver toxicity or liver function abnormality.
• increased bilirubin can indicate liver toxicity or liver function abnormality.
• Single-stranded oligonucleotide means an oligonucleotide which is not hybridized to a
• Specifically hybridizable refers to an antisense compound having a sufficient degree of complementarity between an antisense oligonucleotide and a target nucleic acid to induce a desired effect, while exhibiting minimal or no effects on non-target nucleic acids under conditions in which specific binding is desired, i.e. under physiological conditions in the case of in vivo assays and therapeutic treatments.
• Sttatin means an agent that inhibits the activity of HMG-CoA reductase.
• Subcutaneous administration means administration just below the skin.
• sugar moiety means a naturally occurring sugar moiety or a modified sugar moiety of a nucleoside.
• substituted sugar moiety means a furanosyl that is not a naturally occurring sugar moiety.
• Substituted sugar moieties include, but are not limited to furanosyls comprising substituents at the 2'-position, the 3'-position, the 5'-position and/or the 4'-position.
• Certain substituted sugar moieties are bicyclic sugar moieties.
• sucrose surrogate means a structure that does not comprise a furanosyl and that is capable of replacing the naturally occurring sugar moiety of a nucleoside, such that the resulting nucleoside sub-units are capable of linking together and/or linking to other nucleosides to form an oligomeric compound which is capable of hybridizing to a complementary oligomeric compound.
• Such structures include rings comprising a different number of atoms than furanosyl (e.g., 4, 6, or 7-membered rings); replacement of the oxygen of a furanosyl with a non- oxygen atom (e.g., carbon, sulfur, or nitrogen); or both a change in the number of atoms and a replacement of the oxygen.
• Such structures may also comprise substitutions corresponding to those described for substituted sugar moieties (e.g., 6-membered carbocyclic bicyclic sugar surrogates optionally comprising additional substituents).
• Sugar surrogates also include more complex sugar replacements (e.g., the non-ring systems of peptide nucleic acid).
• Sugar surrogates include without limitation morpholinos, cyclohexenyls and cyclohexitols. "Targeting" or “targeted” means the process of design and selection of an antisense compound that will specifically hybridize to a target nucleic acid and induce a desired effect.
• Target nucleic acid “Target nucleic acid,” “target RNA,” and “target RNA transcript” all refer to a nucleic acid capable of being targeted by antisense compounds.
• Target segment means the sequence of nucleotides of a target nucleic acid to which an antisense compound is targeted.
• 5' target site refers to the 5'-most nucleotide of a target segment.
• 3' target site refers to the 3 '-most nucleotide of a target segment.
• “Therapeutically effective amount” means an amount of an agent that provides a therapeutic benefit to an individual.
• “Therapeutic lifestyle change” means dietary and lifestyle changes intended to lower fat /adipose tissue mass and/or cholesterol. Such change can reduce the risk of developing heart disease, and may includes recommendations for dietary intake of total daily calories, total fat, saturated fat, polyunsaturated fat, monounsaturated fat, carbohydrate, protein, cholesterol, insoluble fiber, as well as recommendations for physical activity.
• “Triglyceride” or “TG” means a lipid or neutral fat consisting of glycerol combined with three fatty acid molecules.
• Type 2 diabetes (also known as “type 2 diabetes mellitus” or “diabetes mellitus, type 2”, and formerly called “diabetes mellitus type 2” , “non-insulin-dependent diabetes (NIDDM)", “obesity related diabetes”, or “adult-onset diabetes”) is a metabolic disorder that is primarily characterized by insulin resistance, relative insulin deficiency, and hyperglycemia.
• NIDDM non-insulin-dependent diabetes
• Treat refers to administering a pharmaceutical composition to an animal to effect an alteration or improvement of a disease, disorder, or condition.
• unmodified nucleobase or “naturally occurring nucleobase” means the naturally occurring heterocyclic nucleobases of RNA or DNA: the purine bases adenine (A) and guanine (G), and the pyrimidine bases thymine (T), cytosine (C) (including 5-methyl C), and uracil (U).
• Unmodified nucleotide means a nucleotide composed of naturally occurring nucleobases, sugar moieties, and internucleoside linkages.
• an unmodified nucleotide is an RNA nucleotide (i.e. ⁇ -D-ribonucleosides) or a DNA nucleotide (i.e. ⁇ -D-deoxyribonucleoside).
• 2'-substituted nucleoside means a nucleoside comprising a substituent at the 2'- position other than H or OH. Unless otherwise indicated, a 2'-substituted nucleoside is not a bicyclic nucleoside.
• deoxynucleoside means a nucleoside comprising 2'-H furanosyl sugar moiety, as found in naturally occurring deoxyribonucleosides (DNA).
• a 2 '-deoxynucleoside may comprise a modified nucleobase or may comprise an RNA nucleobase (e.g., uracil).
• oligonucleotide means a compound comprising a plurality of linked nucleosides.
• an oligonucleotide comprises one or more unmodified ribonucleosides (RNA) and/or unmodified deoxyribonucleosides (DNA) and/or one or more modified nucleosides.
• oligonucleoside means an oligonucleotide in which none of the internucleoside linkages contains a phosphorus atom.
• oligonucleotides include oligonucleosides.
• modified oligonucleotide means an oligonucleotide comprising at least one modified nucleoside and/or at least one modified internucleoside linkage.
• linkage means a group of atoms that link together two or more other groups of atoms.
• nucleoside linkage means a covalent linkage between adjacent nucleosides in an oligonucleotide.
• naturally occurring internucleoside linkage means a 3' to 5' phosphodiester linkage.
• modified internucleoside linkage means any internucleoside linkage other than a naturally occurring internucleoside linkage.
• terminal internucleoside linkage means the linkage between the last two nucleosides of an oligonucleotide or defined region thereof.
• phosphorus linking group means a linking group comprising a phosphorus atom.
• Phosphorus linking groups include without limitation groups having the formula: I
• R a and R d are each, independently, 0, S, CH 3 ⁇ 4 NH, or Ji wherein Ji is Ci-C 6 alkyl or substituted Q- C 6 alkyl;
• R b is 0 or S
• R c is OH, SH, Ci-C 6 alkyl, substituted Ci-C 6 alkyl, Ci-C 6 alkoxy, substituted Ci-C 6 alkoxy, amino or substituted amino;
• Phosphorus linking groups include without limitation, phosphodiester, phosphorothioate, phosphorodithioate, phosphonate, phosphoramidate, phosphorothioamidate, thionoalkylphosphonate, phosphotriesters, thionoalkylphosphotriester and boranophosphate.
• nucleoside phosphorus linking group means a phosphorus linking group that directly links two nucleosides.
• non-internucleoside phosphorus linking group means a phosphorus linking group that does not directly link two nucleosides.
• a non-internucleoside phosphorus linking group links a nucleoside to a group other than a nucleoside.
• a non- internucleoside phosphorus linking group links two groups, neither of which is a nucleoside.
• neutral linking group means a linking group that is not charged.
• Further neutral linking groups include nonionic linkages comprising siloxane (dialkylsiloxane), carboxylate ester, carboxamide, sulfide, sulfonate ester and amides (See for example: Carbohydrate Modifications in Antisense Research; Y.S. Sanghvi and P.D. Cook Eds. ACS Symposium Series 580; Chapters 3 and 4, (pp. 40-65)).
• Further neutral linking groups include nonionic linkages comprising mixed N, O, S and CH 2 component parts.
• nucleoside neutral linking group means a neutral linking group that directly links two nucleosides.
• non-internucleoside neutral linking group means a neutral linking group that does not directly link two nucleosides.
• a non-internucleoside neutral linking group links a nucleoside to a group other than a nucleoside.
• a non-internucleoside neutral linking group links two groups, neither of which is a nucleoside.
• oligomeric compound means a polymeric structure comprising two or more sub- structures.
• an oligomeric compound comprises an oligonucleotide.
• an oligomeric compound comprises one or more conjugate groups and/or terminal groups.
• an oligomeric compound consists of an oligonucleotide. Oligomeric compounds also include naturally occurring nucleic acids.
• an oligomeric compound comprises a backbone of one or more linked monomeric subunits where each linked monomeric subunit is directly or indirectly attached to a heterocyclic base moiety.
• oligomeric compounds may also include monomeric subunits that are not linked to a heterocyclic base moiety, thereby providing abasic sites.
• the linkages joining the monomeric subunits, the sugar moieties or surrogates and the heterocyclic base moieties can be independently modified.
• the linkage-sugar unit, which may or may not include a heterocyclic base may be substituted with a mimetic such as the monomers in peptide nucleic acids.
• terminal group means one or more atom attached to either, or both, the 3 ' end or the 5' end of an oligonucleotide. In certain embodiments a terminal group is a conjugate group. In certain embodiments, a terminal group comprises one or more terminal group nucleosides.
• conjugate means an atom or group of atoms bound to an oligonucleotide or oligomeric compound.
• conjugate groups modify one or more properties of the compound to which they are attached, including, but not limited to pharmacodynamic, pharmacokinetic, binding, absorption, cellular distribution, cellular uptake, charge and/or clearance properties.
• conjugate linker or “linker” in the context of a conjugate group means a portion of a conjugate group comprising any atom or group of atoms and which covalently link (1) an oligonucleotide to another portion of the conjugate group or (2) two or more portions of the conjugate group.
• Conjugate groups are shown herein as radicals, providing a bond for forming covalent attachment to an oligomeric compound such as an antisense oligonucleotide.
• the point of attachment on the oligomeric compound is the 3'-oxygen atom of the 3'-hydroxyl group of the 3' terminal nucleoside of the oligomeric compound.
• the point of attachment on the oligomeric compound is the 5'-oxygen atom of the 5'-hydroxyl group of the 5' terminal nucleoside of the oligomeric compound.
• the bond for forming attachment to the oligomeric compound is a cleavable bond. In certain such embodiments, such cleavable bond constitutes all or part of a cleavable moiety.
• conjugate groups comprise a cleavable moiety (e.g., a cleavable bond or cleavable nucleoside) and a carbohydrate cluster portion, such as a GalNAc cluster portion.
• carbohydrate cluster portion comprises: a targeting moiety and, optionally, a conjugate linker.
• the carbohydrate cluster portion is identified by the number and identity of the ligand. For example, in certain embodiments, the carbohydrate cluster portion comprises 3 GalNAc groups and is designated "GalNAc 3 ". In certain embodiments, the carbohydrate cluster portion comprises 4 GalNAc groups and is designated "GalNAc i".
• carbohydrate cluster portions having specific tether, branching and conjugate linker groups
• GalNac3-l a refers to a specific carbohydrate cluster portion of a conjugate group having 3 GalNac groups and specifically identified tether, branching and linking groups.
• Such carbohydrate cluster fragment is attached to an oligomeric compound via a cleavable moiety, such as a cleavable bond or cleavable nucleoside.
• cleavable moiety means a bond or group that is capable of being split under physiological conditions.
• a cleavable moiety is cleaved inside a cell or sub-cellular compartments, such as a lysosome.
• a cleavable moiety is cleaved by endogenous enzymes, such as nucleases.
• a cleavable moiety comprises a group of atoms having one, two, three, four, or more than four cleavable bonds.
• cleavable bond means any chemical bond capable of being split.
• a cleavable bond is selected from among: an amide, a polyamide, an ester, an ether, one or both esters of a phosphodiester, a phosphate ester, a carbamate, a di-sulfide, or a peptide.
• carbohydrate cluster means a compound having one or more carbohydrate residues attached to a scaffold or linker group, (see, e.g., Maier et al., “Synthesis of Antisense Oligonucleotides Conjugated to a Multivalent Carbohydrate Cluster for Cellular Targeting,” Bioconjugate Chemistry, 2003, (14): 18-29, which is incorporated herein by reference in its entirety, or Rensen et al., “Design and Synthesis of Novel N-Acetylgalactosamine-Terminated Glycolipids for Targeting of Lipoproteins to the Hepatic Asiaglycoprotein Receptor," J. Med. Chem. 2004, (47): 5798-5808, for examples of carbohydrate conjugate clusters).
• modified carbohydrate means any carbohydrate having one or more chemical modifications relative to naturally occurring carbohydrates.
• carbohydrate derivative means any compound which may be synthesized using a carbohydrate as a starting material or intermediate.
• carbohydrate means a naturally occurring carbohydrate, a modified carbohydrate, or a carbohydrate derivative.
• protecting group means any compound or protecting group known to those having skill in the art. Non-limiting examples of protecting groups may be found in "Protective Groups in Organic Chemistry", T. W. Greene, P. G. M. Wuts, ISBN 0-471-62301-6, John Wiley & Sons, Inc, New York, which is incorporated herein by reference in its entirety.
• single-stranded means an oligomeric compound that is not hybridized to its complement and which lacks sufficient self-complementarity to form a stable self-duplex.
• double stranded means a pair of oligomeric compounds that are hybridized to one another or a single self-complementary oligomeric compound that forms a hairpin structure.
• a double-stranded oligomeric compound comprises a first and a second oligomeric compound.
• antisense compound means a compound comprising or consisting of an oligonucleotide at least a portion of which is complementary to a target nucleic acid to which it is capable of hybridizing, resulting in at least one antisense activity.
• antisense activity means any detectable and/or measurable change attributable to the hybridization of an antisense compound to its target nucleic acid.
• antisense activity includes modulation of the amount or activity of a target nucleic acid transcript (e.g. mRNA).
• antisense activity includes modulation of the splicing of pre-mRNA.
• RNase H based antisense compound means an antisense compound wherein at least some of the antisense activity of the antisense compound is attributable to hybridization of the antisense compound to a target nucleic acid and subsequent cleavage of the target nucleic acid by RNase H.
• RISC based antisense compound means an antisense compound wherein at least some of the antisense activity of the antisense compound is attributable to the RNA Induced Silencing Complex (RISC).
• RISC RNA Induced Silencing Complex
• detecting or “measuring” means that a test or assay for detecting or measuring is performed. Such detection and/or measuring may result in a value of zero. Thus, if a test for detection or measuring results in a finding of no activity (activity of zero), the step of detecting or measuring the activity has nevertheless been performed.
• detecttable and/or measureable activity means a statistically significant activity that is not zero.
• essentially unchanged means little or no change in a particular parameter, particularly relative to another parameter which changes much more.
• a parameter is essentially unchanged when it changes less than 5%.
• a parameter is essentially unchanged if it changes less than two-fold while another parameter changes at least ten-fold.
• an antisense activity is a change in the amount of a target nucleic acid.
• the amount of a non-target nucleic acid is essentially unchanged if it changes much less than the target nucleic acid does, but the change need not be zero.
• expression means the process by which a gene ultimately results in a protein.
• Expression includes, but is not limited to, transcription, post-transcriptional modification (e.g., splicing, polyadenlyation, addition of 5 '-cap), and translation.
• target nucleic acid means a nucleic acid molecule to which an antisense compound is intended to hybridize to result in a desired antisense activity.
• Antisense oligonucleotides have sufficient complementarity to their target nucleic acids to allow hybridization under physiological conditions.
• nucleobase complementarity or “complementarity” when in reference to nucleobases means a nucleobase that is capable of base pairing with another nucleobase.
• adenine (A) is com lementary to thymine (T).
• adenine (A) is complementary to uracil (U).
• complementary nucleobase means a nucleobase of an antisense compound that is capable of base pairing with a nucleobase of its target nucleic acid.
• nucleobases at a certain position of an antisense compound are capable of hydrogen bonding with a nucleobase at a certain position of a target nucleic acid
• the position of hydrogen bonding between the oligonucleotide and the target nucleic acid is considered to be complementary at that nucleobase pair.
• Nucleobases comprising certain modifications may maintain the ability to pair with a counterpart nucleobase and thus, are still capable of nucleobase complementarity.
• non-complementary in reference to nucleobases means a pair of nucleobases that do not form hydrogen bonds with one another.
• complementary in reference to oligomeric compounds (e.g., linked nucleosides, oligonucleotides, or nucleic acids) means the capacity of such oligomeric compounds or regions thereof to hybridize to another oligomeric compound or region thereof through nucleobase complementarity.
• Complementary oligomeric compounds need not have nucleobase complementarity at each nucleoside. Rather, some mismatches are tolerated.
• complementary oligomeric compounds or regions are complementary at 70% of the nucleobases (70% complementary).
• complementary oligomeric compounds or regions are 80% complementary.
• complementary oligomeric compounds or regions are 90%> complementary.
• complementary oligomeric compounds or regions are 95% complementary.
• complementary oligomeric compounds or regions are 100% complementary.
• mismatch means a nucleobase of a first oligomeric compound that is not capable of pairing with a nucleobase at a corresponding position of a second oligomeric compound, when the first and second oligomeric compound are aligned.
• first and second oligomeric compounds may be oligonucleotides.
• hybridization means the pairing of complementary oligomeric compounds (e.g., an antisense compound and its target nucleic acid). While not limited to a particular mechanism, the most common mechanism of pairing involves hydrogen bonding, which may be Watson-Crick, Hoogsteen or reversed Hoogsteen hydrogen bonding, between complementary nucleobases.
• oligonucleotide or portion thereof means that each nucleobase of the oligonucleotide or portion thereof is capable of pairing with a nucleobase of a complementary nucleic acid or contiguous portion thereof. Thus, a fully complementary region comprises no mismatches or unhybridized nucleobases in either strand.
• percent complementarity means the percentage of nucleobases of an oligomeric compound that are complementary to an equal-length portion of a target nucleic acid. Percent complementarity is calculated by dividing the number of nucleobases of the oligomeric compound that are complementary to nucleobases at corresponding positions in the target nucleic acid by the total length of the oligomeric compound.
• percent identity means the number of nucleobases in a first nucleic acid that are the same type (independent of chemical modification) as nucleobases at corresponding positions in a second nucleic acid, divided by the total number of nucleobases in the first nucleic acid.
• modulation means a change of amount or quality of a molecule, function, or activity when compared to the amount or quality of a molecule, function, or activity prior to modulation.
• modulation includes the change, either an increase (stimulation or induction) or a decrease (inhibition or reduction) in gene expression.
• modulation of expression can include a change in splice site selection of pre-mRNA processing, resulting in a change in the absolute or relative amount of a particular splice-variant compared to the amount in the absence of modulation.
• chemical motif means a pattern of chemical modifications in an oligonucleotide or a region thereof. Motifs may be defined by modifications at certain nucleosides and/or at certain linking groups of an oligonucleotide.
• nucleoside motif means a pattern of nucleoside modifications in an oligonucleotide or a region thereof.
• the linkages of such an oligonucleotide may be modified or unmodified.
• motifs herein describing only nucleosides are intended to be nucleoside motifs. Thus, in such instances, the linkages are not limited.
• sugar motif means a pattern of sugar modifications in an oligonucleotide or a region thereof.
• linkage motif means a pattern of linkage modifications in an oligonucleotide or region thereof.
• the nucleosides of such an oligonucleotide may be modified or unmodified.
• motifs herein describing only linkages are intended to be linkage motifs. Thus, in such instances, the nucleosides are not limited.
• nucleobase modification motif means a pattern of modifications to nucleobases along an oligonucleotide. Unless otherwise indicated, a nucleobase modification motif is independent of the nucleobase sequence.
• sequence motif means a pattern of nucleobases arranged along an oligonucleotide or portion thereof. Unless otherwise indicated, a sequence motif is independent of chemical modifications and thus may have any combination of chemical modifications, including no chemical modifications.
• nucleoside having a modification of a first type may be an unmodified nucleoside.
• telomeres As used herein, “differently modified” mean chemical modifications or chemical substituents that are different from one another, including absence of modifications. Thus, for example, a MOE nucleoside and an unmodified DNA nucleoside are “differently modified,” even though the DNA nucleoside is unmodified. Likewise, DNA and RNA are “differently modified,” even though both are naturally- occurring unmodified nucleosides. Nucleosides that are the same but for comprising different nucleobases are not differently modified.
• nucleoside comprising a 2'-OMe modified sugar and an unmodified adenine nucleobase and a nucleoside comprising a 2'-OMe modified sugar and an unmodified thymine nucleobase are not differently modified.
• the same type of modifications refers to modifications that are the same as one another, including absence of modifications.
• two unmodified DNA nucleosides have “the same type of modification,” even though the DNA nucleoside is unmodified.
• Such nucleosides having the same type modification may comprise different nucleobases.
• separate regions means portions of an oligonucleotide wherein the chemical modifications or the motif of chemical modifications of any neighboring portions include at least one difference to allow the separate regions to be distinguished from one another.
• pharmaceutically acceptable carrier or diluent means any substance suitable for use in administering to an animal.
• a pharmaceutically acceptable carrier or diluent is sterile saline.
• such sterile saline is pharmaceutical grade saline.
• metabolic disorder means a disease or condition principally characterized by dysregulation of metabolism - the complex set of chemical reactions associated with breakdown of food to produce energy.
• cardiovascular disorder means a disease or condition principally characterized by impaired function of the heart or blood vessels.
• mono or polycyclic ring system is meant to include all ring systems selected from single or polycyclic radical ring systems wherein the rings are fused or linked and is meant to be inclusive of single and mixed ring systems individually selected from aliphatic, alicyclic, aryl, heteroaryl, aralkyl, arylalkyl, heterocyclic, heteroaryl, heteroaromatic and heteroarylalkyl.
• Such mono and poly cyclic structures can contain rings that each have the same level of saturation or each, independently, have varying degrees of saturation including fully saturated, partially saturated or fully unsaturated.
• Each ring can comprise ring atoms selected from C, N, 0 and S to give rise to heterocyclic rings as well as rings comprising only C ring atoms which can be present in a mixed motif such as for example benzimidazole wherein one ring has only carbon ring atoms and the fused ring has two nitrogen atoms.
• Mono or polycyclic ring systems can be attached to parent molecules using various strategies such as directly through a ring atom, fused through multiple ring atoms, through a substituent group or through a bifunctional linking moiety.
• prodrug means an inactive or less active form of a compound which, when administered to a subject, is metabolized to form the active, or more active, compound (e.g., drug).
• substituted nucleoside and “substituent group,” means an atom or group that replaces the atom or group of a named parent compound.
• a substituent of a modified nucleoside is any atom or group that differs from the atom or group found in a naturally occurring nucleoside (e.g., a modified 2'- substuent is any atom or group at the 2'-position of a nucleoside other than H or OH).
• Substituent groups can be protected or unprotected.
• compounds of the present disclosure have substituents at one or at more than one position of the parent compound. Substituents may also be further substituted with other substituent groups and may be attached directly or via a linking group such as an alkyl or hydrocarbyl group to a parent compound.
• substituted in reference to a chemical functional group means an atom or group of atoms that differs from the atom or a group of atoms normally present in the named functional group.
• a substituent replaces a hydrogen atom of the functional group (e.g., in certain embodiments, the substituent of a substituted methyl group is an atom or group other than hydrogen which replaces one of the hydrogen atoms of an unsubstituted methyl group).
• each R ⁇ , R bb and R cc is, independently, H, an optionally linked chemical functional group or a further substituent group with a preferred list including without limitation, alkyl, alkenyl, alkynyl, aliphatic, alkoxy, acyl, aryl, aralkyl, heteroaryl, alicyclic, heterocyclic and heteroarylalkyl. Selected substituents within the compounds described herein are present to a recursive degree.
• alkyl means a saturated straight or branched hydrocarbon radical containing up to twenty four carbon atoms.
• alkyl groups include without limitation, methyl, ethyl, propyl, butyl, isopropyl, n-hexyl, octyl, decyl, dodecyl and the like.
• Alkyl groups typically include from 1 to about 24 carbon atoms, more typically from 1 to about 12 carbon atoms (C 1 -C 12 alkyl) with from 1 to about 6 carbon atoms being more preferred.
• alkenyl means a straight or branched hydrocarbon chain radical containing up to twenty four carbon atoms and having at least one carbon-carbon double bond.
• alkenyl groups include without limitation, ethenyl, propenyl, butenyl, l-methyl-2-buten-l-yl, dienes such as 1,3 -butadiene and the like.
• Alkenyl groups typically include from 2 to about 24 carbon atoms, more typically from 2 to about 12 carbon atoms with from 2 to about 6 carbon atoms being more preferred.
• Alkenyl groups as used herein may optionally include one or more further substituent groups.
• alkynyl means a straight or branched hydrocarbon radical containing up to twenty four carbon atoms and having at least one carbon-carbon triple bond.
• alkynyl groups include, without limitation, ethynyl, 1-propynyl, 1-butynyl, and the like.
• Alkynyl groups typically include from 2 to about 24 carbon atoms, more typically from 2 to about 12 carbon atoms with from 2 to about 6 carbon atoms being more preferred.
• Alkynyl groups as used herein may optionally include one or more further substituent groups.
• acyl means a radical formed by removal of a hydroxyl group from an organic acid and has the general Formula -C(0)-X where X is typically aliphatic, alicyclic or aromatic. Examples include aliphatic carbonyls, aromatic carbonyls, aliphatic sulfonyls, aromatic sulfmyls, aliphatic sulfmyls, aromatic phosphates, aliphatic phosphates and the like. Acyl groups as used herein may optionally include further substituent groups.
• alicyclic means a cyclic ring system wherein the ring is aliphatic.
• the ring system can comprise one or more rings wherein at least one ring is aliphatic.
• Preferred alicyclics include rings having from about 5 to about 9 carbon atoms in the ring.
• Alicyclic as used herein may optionally include further substituent groups.
• aliphatic means a straight or branched hydrocarbon radical containing up to twenty four carbon atoms wherein the saturation between any two carbon atoms is a single, double or triple bond.
• An aliphatic group preferably contains from 1 to about 24 carbon atoms, more typically from 1 to about 12 carbon atoms with from 1 to about 6 carbon atoms being more preferred.
• the straight or branched chain of an aliphatic group may be interrupted with one or more heteroatoms that include nitrogen, oxygen, sulfur and phosphorus.
• Such aliphatic groups interrupted by heteroatoms include without limitation, polyalkoxys, such as polyalkylene glycols, polyamines, and polyimines. Aliphatic groups as used herein may optionally include further substituent groups.
• alkoxy means a radical formed between an alkyl group and an oxygen atom wherein the oxygen atom is used to attach the alkoxy group to a parent molecule.
• alkoxy groups include without limitation, methoxy, ethoxy, propoxy, isopropoxy, n-butoxy, sec-butoxy, feri-butoxy, n- pentoxy, neopentoxy, n-hexoxy and the like.
• Alkoxy groups as used herein may optionally include further substituent groups.
• aminoalkyl means an amino substituted Ci-Ci2 alkyl radical. The alkyl portion of the radical forms a covalent bond with a parent molecule. The amino group can be located at any position and the aminoalkyl group can be substituted with a further substituent group at the alkyl and/or amino portions.
• aralkyl and arylalkyl mean an aromatic group that is covalently linked to a C1-C12 alkyl radical.
• the alkyl radical portion of the resulting aralkyl (or arylalkyl) group forms a covalent bond with a parent molecule. Examples include without limitation, benzyl, phenethyl and the like.
• Aralkyl groups as used herein may optionally include further substituent groups attached to the alkyl, the aryl or both groups that form the radical group.
• aryl and aromatic mean a mono- or polycyclic carbocyclic ring system radicals having one or more aromatic rings.
• aryl groups include without limitation, phenyl, naphthyl, tetrahydronaphthyl, indanyl, idenyl and the like.
• Preferred aryl ring systems have from about 5 to about 20 carbon atoms in one or more rings.
• Aryl groups as used herein may optionally include further substituent groups.
• heteroaryl and “heteroaromatic,” mean a radical comprising a mono- or polycyclic aromatic ring, ring system or fused ring system wherein at least one of the rings is aromatic and includes one or more heteroatoms. Heteroaryl is also meant to include fused ring systems including systems where one or more of the fused rings contain no heteroatoms. Heteroaryl groups typically include one ring atom selected from sulfur, nitrogen or oxygen.
• heteroaryl groups include without limitation, pyridinyl, pyrazinyl, pyrimidinyl, pyrrolyl, pyrazolyl, imidazolyl, thiazolyl, oxazolyl, isooxazolyl, thiadiazolyl, oxadiazolyl, thiophenyl, furanyl, quinolinyl, isoquinolinyl, benzimidazolyl, benzooxazolyl, quinoxalinyl and the like.
• Heteroaryl radicals can be attached to a parent molecule directly or through a linking moiety such as an aliphatic group or hetero atom.
• Heteroaryl groups as used herein may optionally include further substituent groups.
• conjugate compound means any atoms, group of atoms, or group of linked atoms suitable for use as a conjugate group.
• conjugate compounds may possess or impart one or more properties, including, but not limited to pharmacodynamic, pharmacokinetic, binding, absorption, cellular distribution, cellular uptake, charge and/or clearance properties.
• double-stranded refers to two separate oligomeric compounds that are hybridized to one another. Such double stranded compounds may have one or more or non-hybridizing nucleosides at one or both ends of one or both strands (overhangs) and'or one or more internal non-hybridizing nucleosides (mismatches) provided there is sufficient complementarity to maintain hybridization under physiologically relevant conditions.
• the invention provides conjugated antisense compounds comprising antisense oligonucleoitdes and a conjugate. a. Certain Antisense Oligonucleotides
• the invention provides antisense oligonucleotides.
• antisense oligonucleotides comprise linked nucleosides, each nucleoside comprising a sugar moiety and a nucleobase.
• the structure of such antisense oligonucleotides may be considered in terms of chemical features (e.g., modifications and patterns of modifications) and nucleobase sequence (e.g., sequence of antisense oligonucleotide, idenity and sequence of target nucleic acid),
• antisense oligonucleotide comprise one or more modification.
• antisense oligonucleotides comprise one or more modified nucleosides and/or modified internucleoside linkages.
• modified nucleosides comprise a modifed sugar moirty and/or modifed nucleobase.
• compounds of the disclosure comprise one or more modifed nucleosides comprising a modifed sugar moiety.
• Such compounds comprising one or more sugar-modified nucleosides may have desirable properties, such as enhanced nuclease stability or increased binding affinity with a target nucleic acid relative to an oligonucleotide comprising only nucleosides comprising naturally occurring sugar moieties.
• modified sugar moieties are substitued sugar moieties.
• modified sugar moieties are sugar surrogates. Such sugar surrogates may comprise one or more substitutions corresponding to those of substituted sugar moieties.
• modified sugar moieties are substituted sugar moieties comprising one or more non-bridging sugar substituent, including but not limited to substituents at the 2' and/or 5' positions.
• sugar substituents suitable for the 2'-position include, but are not limited to: 2'-F, 2'-OCH 3 ("OMe” or "O-methyl"), and 2'-0(CH 2 ) 2 OCH 3 (“MOE").
• sugar substituents at the 5'- position include, but are not limited to:, 5'-methyl (R or S); 5'-vinyl, and 5'-methoxy.
• substituted sugars comprise more than one non-bridging sugar substituent, for example, 2'-F- 5'-methyl sugar moieties (see,e.g., PCT International Application WO 2008/101157, for additional 5', 2'-bis substituted sugar moieties and nucleosides).
• Nucleosides comprising 2 '-substituted sugar moieties are referred to as 2'-substituted nucleosides.
• These 2'-substituent groups can be further substituted with one or more substituent groups independently selected from hydroxyl, amino, alkoxy, carboxy, benzyl, phenyl, nitro (N0 2 ), thiol, thioalkoxy (S-alkyl), halogen, alkyl, aryl, alkenyl and alkynyl.
• a 2'- substituted nucleoside comprises a sugar moiety comprising a 2'- substituent group selected from F, 0-CH 3 , and OCH 2 CH 2 OCH 3 .
• Certain modifed sugar moieties comprise a bridging sugar substituent that forms a second ring resulting in a bicyclic sugar moiety.
• the bicyclic sugar moiety comprises a bridge between the 4' and the 2' furanose ring atoms.
• Examples of such 4' to 2' sugar substituents include, but are not limited to: -[C(R a )(R b )] n -, -[C(R a )(R b )] n -0-, -C(R a R b )-N(R)-0- or, -C(R a R b )-0-N(R)-; 4'-CH 2 -2', 4'-(CH 2 ) 2 -2', 4'-(CH 2 ) 3 -2',.
• Patent 7,427,672 issued on September 23, 2008
• 4'-C3 ⁇ 4- C(H)(CH 3 )-2' see, e.g., Chattopadhyaya, et al, J. Org. Chem.,2009, 74, 118-134
• x 0, 1, or 2;
• n 1, 2, 3, or 4;
• Bicyclic nucleosides include, but are not limited to, (A) o.-L-Methyleneoxy (4'-CH 2 -0-2') BNA , (B) ⁇ -D- Methyleneoxy (4'-CH 2 -0-2') BNA (also referred to as locked nucleic acid or LNA) , (C) Ethyleneoxy (4'- (CH 2 ) 2 -0-2') BNA , (D) Aminooxy (4'-CH 2 -0-N(R)-2') BNA, (E) Oxyamino (4'-CH 2 -N(R)-0-2') BNA, (F) Methyl(methyleneoxy) (4'-CH(CH 3 )-0-2') BNA (also referred to as constrained ethyl or cEt), (G) methylene-thio (4'-CH
• Bx is a nucleobase moiety and R is, independently, H, a protecting group, or C1-C12 alkyl.
• bicyclic sugar moieties and nucleosides incorporating such bicyclic sugar moieties are further defined by isomeric configuration.
• a nucleoside comprising a 4'-2' methylene-oxy bridge may be in the a-L configuration or in the ⁇ -D configuration.
• a-L- methyleneoxy (4'-CH 2 -0-2') bicyclic nucleosides have been incorporated into antisense oligonucleotides that showed antisense activity (Frieden et al, Nucleic Acids Research, 2003, 21, 6365-6372).
• substituted sugar moieties comprise one or more non-bridging sugar substituent and one or more bridging sugar substituent (e.g., 5 '-substituted and 4'-2' bridged sugars), (see, PCT International Application WO 2007/134181, published on 11/22/07, wherein LNA is substituted with, for example, a 5 '-methyl or a 5 '-vinyl group).
• bridging sugar substituent e.g., 5 '-substituted and 4'-2' bridged sugars
• modified sugar moieties are sugar surrogates.
• the oxygen atom of the naturally occuring sugar is substituted, e.g., with a sulfer, carbon or nitrogen atom.
• such modified sugar moiety also comprises bridging and/or non-bridging substituents as described above.
• certain sugar surrogates comprise a 4'-sulfer atom and a substitution at the 2'-position (see,Q.g., published U.S. Patent Application US2005/0130923, published on June 16, 2005) and/or the 5' position.
• carbocyclic bicyclic nucleosides having a 4'-2' bridge have been described (see, e.g., Freier et al, Nucleic Acids Research, 1997, 25(22), 4429-4443 and Albaek ei fl/., J. Org. Chem., 2006, 71, 7731-7740).
• sugar surrogates comprise rings having other than 5-atoms.
• a sugar surrogate comprises a morphlino. Morpholino compounds and their use in oligomeric compounds has been reported in numerous patents and published articles (see for example:
• morpholino means a sugar surrogate having the following structure:
• morpholinos may be modified, for example by adding or altering various substituent groups from the above morpholino structure.
• sugar surrogates are refered to herein as "modifed morpholinos.”
• a sugar surrogate comprises a six-membered tetrahydropyran.
• Such tetrahydropyrans may be further modified or substituted.
• Nucleosides comprising such modified tetrahydropyrans include, but are not limited to, hexitol nucleic acid (HNA), anitol nucleic acid (ANA), manitol nucleic acid (MNA) (see Leumann, CJ. Bioorg. & Med. Chem. (2002) 10:841-854), fluoro HNA (F-HNA), and those compounds having Formula VI:
• Bx is a nucleobase moiety
• T3 and T4 are each, independently, an intemucleoside linking group linking the tetrahydropyran nucleoside analog to the antisense compound or one of T 3 and T 4 is an intemucleoside linking group linking the tetrahydropyran nucleoside analog to the antisense compound and the other of T3 and T4 is H, a hydroxyl protecting group, a linked conjugate group, or a 5' or 3'-terminal group;
• the modified THP nucleosides of Formula VI are provided wherein qi, q 2 , q 3 , q 4 , q 5 , q 6 and q 7 are each H. In certain embodiments, at least one of qi, q 2 , q 3 , q 4 , qs, q6 and q 7 is other than H. In certain embodiments, at least one of qi, q 2 , q 3 , q 4 , qs, qe and q 7 is methyl. In certain embodiments, THP nucleosides of Formula VI are provided wherein one of Ri and R 2 is F. In certain embodiments, Ri is fluoro and R 2 is H, Ri is methoxy and R 2 is H, and Ri is methoxyethoxy and R 2 is H.
• Patent Application US2005-0130923, published on June 16, 2005) or alternatively 5'-substitution of a bicyclic nucleic acid see PCT International Application WO 2007/134181, published on 11/22/07 wherein a 4'-CH 2 -0-2' bicyclic nucleoside is further substituted at the 5' position with a 5'-methyl or a 5'-vinyl group).
• PCT International Application WO 2007/134181 published on 11/22/07 wherein a 4'-CH 2 -0-2' bicyclic nucleoside is further substituted at the 5' position with a 5'-methyl or a 5'-vinyl group.
• carbocyclic bicyclic nucleosides along with their oligomerization and biochemical studies have also been described (see, e.g., Srivastava et al, J. Am. Chem. Soc. 2007, 129(26), 8362-8379).
• the present disclosure provides oligonucleotides comprising modified nucleosides.
• modified nucleotides may include modified sugars, modified nucleobases, and/or modified linkages. The specific modifications are selected such that the resulting oligonucleotides possess desireable characteristics.
• oligonucleotides comprise one or more RNA-like nucleosides. In certain embodiments, oligonucleotides comprise one or more DNA-like nucleotides.
• nucleosides of the present disclosure comprise one or more unmodified nucleobases. In certain embodiments, nucleosides of the present disclosure comprise one or more modifed nucleobases.
• modified nucleobases are selected from: universal bases, hydrophobic bases, promiscuous bases, size-expanded bases, and fluorinated bases as defined herein.
• nucleobases include tricyclic pyrimidines such as phenoxazine cytidine( [5,4-b][l,4]benzoxazin- 2(3H)-one), phenothiazine cytidine (lH-pyrimido[5,4-b][l,4]benzothiazin-2(3H)-one), G-clamps such as a substituted phenoxazine cytidine (e.g.
• nucleobases may also include those in which the purine or pyrimidine base is replaced with other heterocycles, for example 7-deaza-adenine, 7-deazaguanosine, 2-aminopyridine and 2- pyridone.
• nucleobases include those disclosed in United States Patent No. 3,687,808, those disclosed in The Concise Encyclopedia Of Polymer Science And Engineering, Kroschwitz, J.I., Ed., John Wiley & Sons, 1990, 858-859; those disclosed by Englisch et al, Angewandte Chemie, International Edition, 1991, 30, 613; and those disclosed by Sanghvi, Y.S., Chapter 15, Antisense Research and Applications, Crooke, S.T. and Lebleu, B., Eds., CRC Press, 1993, 273-288.
• the present disclosure provides oligonucleotides comprising linked nucleosides.
• nucleosides may be linked together using any internucleoside linkage.
• the two main classes of internucleoside linking groups are defined by the presence or absence of a phosphorus atom.
• Representative phosphorus containing internucleoside linkages include, but are not limited to, phosphodiesters (PO), phosphotriesters, methylphosphonates, phosphoramidate, and phosphorothioates (PS).
• Non-phosphorus containing internucleoside linking groups include, but are not limited to, methylenemethylimino (-CH2-N(CH 3 )-0-CH 2 -), thiodiester (-O-C(O)-S-), thionocarbamate (-0- C(0)(NH)-S-); siloxane (-0-Si(H) 2 -0-); and ⁇ , ⁇ '-dimethylhydrazine (-CH 2 -N(CH 3 )-N(CH 3 )-).
• Modified linkages compared to natural phosphodiester linkages, can be used to alter, typically increase, nuclease resistance of the oligonucleotide.
• internucleoside linkages having a chiral atom can be prepared as a racemic mixture, or as separate enantiomers.
• Representative chiral linkages include, but are not limited to, alkylphosphonates and phosphorothioates. Methods of preparation of phosphorous-containing and non-phosphorous-containing internucleoside linkages are well known to those skilled in the art.
• oligonucleotides described herein contain one or more asymmetric centers and thus give rise to enantiomers, diastereomers, and other stereoisomeric configurations that may be defined, in terms of absolute stereochemistry, as (R) or (S), a or ⁇ such as for sugar anomers, or as (D) or (L) such as for amino acids etc. Included in the antisense compounds provided herein are all such possible isomers, as well as their racemic and optically pure forms.
• Further neutral internucleoside linkages include nonionic linkages comprising siloxane (dialkylsiloxane), carboxylate ester, carboxamide, sulfide, sulfonate ester and amides (See for example: Carbohydrate Modifications in Antisense Research; Y.S. Sanghvi and P.D. Cook, Eds., ACS Symposium Series 580; Chapters 3 and 4, 40-65). Further neutral internucleoside linkages include nonionic linkages comprising mixed N, 0, S and CH 2 component parts.
• antisense oligonucleotides comprise one or more modified nucleoside (e.g., nucleoside comprising a modified sugar and/or modified nucleobase) and/or one or more modified internucleoside linkage.
• modified nucleoside e.g., nucleoside comprising a modified sugar and/or modified nucleobase
• internucleoside linkage e.g., a modified internucleoside linkage.
• the pattern of such modifications on an oligonucleotide is referred to herein as a motif.
• sugar, nucleobase, and linkage motifs are independent of one another.
• oligonucleotides comprise one or more type of modified sugar moieties and/or naturally occurring sugar moieties arranged along an oligonucleotide or region thereof in a defined pattern or sugar modification motif.
• Such motifs may include any of the sugar modifications discussed herein and/or other known sugar modifications.
• the oligonucleotides comprise or consist of a region having a gapmer sugar motif, which comprises two external regions or "wings" and a central or internal region or "gap."
• the three regions of a gapmer sugar motif (the 5 '-wing, the gap, and the 3 '-wing) form a contiguous sequence of nucleosides wherein at least some of the sugar moieties of the nucleosides of each of the wings differ from at least some of the sugar moieties of the nucleosides of the gap.
• the sugar moieties of the nucleosides of each wing that are closest to the gap differ from the sugar moiety of the neighboring gap nucleosides, thus defining the boundary between the wings and the gap.
• the sugar moieties within the gap are the same as one another.
• the gap includes one or more nucleoside having a sugar moiety that differs from the sugar moiety of one or more other nucleosides of the gap.
• the sugar motifs of the two wings are the same as one another (symmetric sugar gapmer).
• the sugar motifs of the 5'-wing differs from the sugar motif of the 3'-wing (asymmetric sugar gapmer).
• the 5'- wing of a gapmer consists of 1 to 8 linked nucleosides. In certain embodiments, the 5'- wing of a gapmer consists of 1 to 7 linked nucleosides. In certain embodiments, the 5'- wing of a gapmer consists of 1 to 6 linked nucleosides. In certain embodiments, the 5'- wing of a gapmer consists of 1 to 5 linked nucleosides. In certain embodiments, the 5'- wing of a gapmer consists of 2 to 5 linked nucleosides. In certain embodiments, the 5'- wing of a gapmer consists of 3 to 5 linked nucleosides.
• the 5'- wing of a gapmer consists of 4 or 5 linked nucleosides. In certain embodiments, the 5'- wing of a gapmer consists of 1 to 4 linked nucleosides. In certain embodiments, the 5'- wing of a gapmer consists of 1 to 3 linked nucleosides. In certain embodiments, the 5'- wing of a gapmer consists of 1 or 2 linked nucleosides. In certain embodiments, the 5'- wing of a gapmer consists of 2 to 4 linked nucleosides. In certain embodiments, the 5'- wing of a gapmer consists of 2 or 3 linked nucleosides.
• the 5'- wing of a gapmer consists of 3 or 4 linked nucleosides. In certain embodiments, the 5'- wing of a gapmer consists of 1 nucleoside. In certain embodiments, the 5'- wing of a gapmer consists of 2 linked nucleosides. In certain embodiments, the 5'- wing of a gapmer consists of 3 linked nucleosides. In certain embodiments, the 5'- wing of a gapmer consists of 4 linked nucleosides. In certain embodiments, the 5'- wing of a gapmer consists of 5 linked nucleosides. In certain embodiments, the 5'- wing of a gapmer consists of 6 linked nucleosides.
• the 5'- wing of a gapmer comprises at least one bicyclic nucleoside. In certain embodiments, the 5'- wing of a gapmer comprises at least two bicyclic nucleosides. In certain embodiments, the 5'- wing of a gapmer comprises at least three bicyclic nucleosides. In certain
• the 5'- wing of a gapmer comprises at least four bicyclic nucleosides. In certain embodiments, the 5'- wing of a gapmer comprises at least one constrained ethyl nucleoside. In certain embodiments, the 5'- wing of a gapmer comprises at least one LNA nucleoside. In certain embodiments, each nucleoside of the 5'- wing of a gapmer is a bicyclic nucleoside. In certain embodiments, each nucleoside of the 5'- wing of a gapmer is a constrained ethyl nucleoside. In certain embodiments, each nucleoside of the 5'- wing of a gapmer is a LNA nucleoside.
• the 5'- wing of a gapmer comprises at least one non-bicyclic modified nucleoside. In certain embodiments, the 5'- wing of a gapmer comprises at least one 2'-substituted nucleoside. In certain embodiments, the 5'- wing of a gapmer comprises at least one 2'-MOE nucleoside. In certain embodiments, the 5'- wing of a gapmer comprises at least one 2'-OMe nucleoside. In certain embodiments, each nucleoside of the 5'- wing of a gapmer is a non-bicyclic modified nucleoside.
• each nucleoside of the 5'- wing of a gapmer is a 2'-substituted nucleoside. In certain embodiments, each nucleoside of the 5'- wing of a gapmer is a 2'-MOE nucleoside. In certain embodiments, each nucleoside of the 5'- wing of a gapmer is a 2'-OMe nucleoside.
• the 5'- wing of a gapmer comprises at least one 2'-deoxynucleoside. In certain embodiments, each nucleoside of the 5'- wing of a gapmer is a 2'-deoxynucleoside. In a certain embodiments, the 5'- wing of a gapmer comprises at least one ribonucleoside. In certain embodiments, each nucleoside of the 5'- wing of a gapmer is a ribonucleoside. In certain embodiments, one, more than one, or each of the nucleosides of the 5'- wing is an RNA-like nucleoside.
• the 5'-wing of a gapmer comprises at least one bicyclic nucleoside and at least one non-bicyclic modified nucleoside. In certain embodiments, the 5'-wing of a gapmer comprises at least one bicyclic nucleoside and at least one 2 '-substituted nucleoside. In certain embodiments, the 5 '-wing of a gapmer comprises at least one bicyclic nucleoside and at least one 2'-MOE nucleoside. In certain embodiments, the 5'-wing of a gapmer comprises at least one bicyclic nucleoside and at least one 2'-OMe nucleoside. In certain embodiments, the 5'-wing of a gapmer comprises at least one bicyclic nucleoside and at least one 2'-deoxynucleoside.
• the 5'-wing of a gapmer comprises at least one constrained ethyl nucleoside and at least one non-bicyclic modified nucleoside. In certain embodiments, the 5 '-wing of a gapmer comprises at least one constrained ethyl nucleoside and at least one 2 '-substituted nucleoside. In certain embodiments, the 5'-wing of a gapmer comprises at least one constrained ethyl nucleoside and at least one 2'-MOE nucleoside. In certain embodiments, the 5'-wing of a gapmer comprises at least one constrained ethyl nucleoside and at least one 2'-OMe nucleoside. In certain embodiments, the 5'-wing of a gapmer comprises at least one constrained ethyl nucleoside and at least one 2'-deoxynucleoside.
• the 3'- wing of a gapmer consists of 1 to 8 linked nucleosides. In certain embodiments, the 3'- wing of a gapmer consists of 1 to 7 linked nucleosides. In certain embodiments, the 3'- wing of a gapmer consists of 1 to 6 linked nucleosides. In certain embodiments, the 3'- wing of a gapmer consists of 1 to 5 linked nucleosides. In certain embodiments, the 3'- wing of a gapmer consists of 2 to 5 linked nucleosides. In certain embodiments, the 3'- wing of a gapmer consists of 3 to 5 linked nucleosides.
• the 3'- wing of a gapmer consists of 4 or 5 linked nucleosides. In certain embodiments, the 3'- wing of a gapmer consists of 1 to 4 linked nucleosides. In certain embodiments, the 3'- wing of a gapmer consists of 1 to 3 linked nucleosides. In certain embodiments, the 3'- wing of a gapmer consists of 1 or 2 linked nucleosides. In certain embodiments, the 3'- wing of a gapmer consists of 2 to 4 linked nucleosides. In certain embodiments, the 3'- wing of a gapmer consists of 2 or 3 linked nucleosides.
• the 3'- wing of a gapmer consists of 3 or 4 linked nucleosides. In certain embodiments, the 3'- wing of a gapmer consists of 1 nucleoside. In certain embodiments, the 3'- wing of a gapmer consists of 2 linked nucleosides. In certain embodiments, the 3'- wing of a gapmer consists of 31inked nucleosides. In certain embodiments, the 3'- wing of a gapmer consists of 4 linked nucleosides. In certain embodiments, the 3'- wing of a gapmer consists of 5 linked nucleosides. In certain embodiments, the 3'- wing of a gapmer consists of 6 linked nucleosides.
• the 3'- wing of a gapmer comprises at least one bicyclic nucleoside. In certain embodiments, the 3'- wing of a gapmer comprises at least one constrained ethyl nucleoside. In certain embodiments, the 3'- wing of a gapmer comprises at least one LNA nucleoside. In certain embodiments, each nucleoside of the 3'- wing of a gapmer is a bicyclic nucleoside. In certain embodiments, each nucleoside of the 3'- wing of a gapmer is a constrained ethyl nucleoside. In certain embodiments, each nucleoside of the 3'- wing of a gapmer is a LNA nucleoside.
• the 3'- wing of a gapmer comprises at least one non-bicyclic modified nucleoside. In certain embodiments, the 3'- wing of a gapmer comprises at least two non-bicyclic modified nucleosides. In certain embodiments, the 3'- wing of a gapmer comprises at least three non-bicyclic modified nucleosides. In certain embodiments, the 3'- wing of a gapmer comprises at least four non-bicyclic modified nucleosides. In certain embodiments, the 3'- wing of a gapmer comprises at least one 2 '-substituted nucleoside.
• the 3'- wing of a gapmer comprises at least one 2'-MOE nucleoside. In certain embodiments, the 3'- wing of a gapmer comprises at least one 2'-OMe nucleoside. In certain embodiments, each nucleoside of the 3'- wing of a gapmer is a non-bicyclic modified nucleoside. In certain embodiments, each nucleoside of the 3'- wing of a gapmer is a 2'-substituted nucleoside. In certain embodiments, each nucleoside of the 3'- wing of a gapmer is a 2'-MOE nucleoside. In certain embodiments, each nucleoside of the 3'- wing of a gapmer is a 2'-OMe nucleoside.
• the 3'- wing of a gapmer comprises at least one 2'-deoxynucleoside. In certain embodiments, each nucleoside of the 3'- wing of a gapmer is a 2'-deoxynucleoside. In a certain embodiments, the 3'- wing of a gapmer comprises at least one ribonucleoside. In certain embodiments, each nucleoside of the 3'- wing of a gapmer is a ribonucleoside. In certain embodiments, one, more than one, or each of the nucleosides of the 5'- wing is an NA-like nucleoside.
• the 3 '-wing of a gapmer comprises at least one bicyclic nucleoside and at least one non-bicyclic modified nucleoside. In certain embodiments, the 3 '-wing of a gapmer comprises at least one bicyclic nucleoside and at least one 2 '-substituted nucleoside. In certain embodiments, the 3'-wing of a gapmer comprises at least one bicyclic nucleoside and at least one 2'-MOE nucleoside. In certain embodiments, the 3'-wing of a gapmer comprises at least one bicyclic nucleoside and at least one 2'-OMe nucleoside. In certain embodiments, the 3 '-wing of a gapmer comprises at least one bicyclic nucleoside and at least one 2'-deoxynucleoside.
• the 3 '-wing of a gapmer comprises at least one constrained ethyl nucleoside and at least one non-bicyclic modified nucleoside. In certain embodiments, the 3 '-wing of a gapmer comprises at least one constrained ethyl nucleoside and at least one 2 '-substituted nucleoside. In certain embodiments, the 3'-wing of a gapmer comprises at least one constrained ethyl nucleoside and at least one
• the 3'-wing of a gapmer comprises at least one constrained ethyl nucleoside and at least one 2'-OMe nucleoside. In certain embodiments, the 3 '-wing of a gapmer comprises at least one constrained ethyl nucleoside and at least one 2'-deoxynucleoside.
• the 3 '-wing of a gapmer comprises at least one LNA nucleoside and at least one non-bicyclic modified nucleoside. In certain embodiments, the 3 '-wing of a gapmer comprises at least one LNA nucleoside and at least one 2 '-substituted nucleoside. In certain embodiments, the 3 '-wing of a gapmer comprises at least one LNA nucleoside and at least one 2'-MOE nucleoside. In certain embodiments, the 3 '-wing of a gapmer comprises at least one LNA nucleoside and at least one 2'-OMe nucleoside. In certain embodiments, the 3 '-wing of a gapmer comprises at least one LNA nucleoside and at least one 2'- deoxynucleoside.
• the 3 '-wing of a gapmer comprises at least one bicyclic nucleoside, at least one non-bicyclic modified nucleoside, and at least one 2'-deoxynucleoside. In certain embodiments, the 3'- wing of a gapmer comprises at least one constrained ethyl nucleoside, at least one non-bicyclic modified nucleoside, and at least one 2'-deoxynucleoside. In certain embodiments, the 3'-wing of a gapmer comprises at least one LNA nucleoside, at least one non-bicyclic modified nucleoside, and at least one 2'- deoxynucleoside.
• the 3 '-wing of a gapmer comprises at least one bicyclic nucleoside, at least one 2 '-substituted nucleoside, and at least one 2'-deoxynucleoside. In certain embodiments, the 3'-wing of a gapmer comprises at least one constrained ethyl nucleoside, at least one 2 '-substituted nucleoside, and at least one 2'-deoxynucleoside. In certain embodiments, the 3'-wing of a gapmer comprises at least one LNA nucleoside, at least one 2'-substituted nucleoside, and at least one 2'-deoxynucleoside.
• the 3 '-wing of a gapmer comprises at least one bicyclic nucleoside, at least one 2'-MOE nucleoside, and at least one 2'-deoxynucleoside. In certain embodiments, the 3 '-wing of a gapmer comprises at least one constrained ethyl nucleoside, at least one 2'-MOE nucleoside, and at least one 2'-deoxynucleoside. In certain embodiments, the 3'-wing of a gapmer comprises at least one LNA nucleoside, at least one 2'-MOE nucleoside, and at least one 2'-deoxynucleoside.
• the 3 '-wing of a gapmer comprises at least one bicyclic nucleoside, at least one 2'-OMe nucleoside, and at least one 2'-deoxynucleoside. In certain embodiments, the 3 '-wing of a gapmer comprises at least one constrained ethyl nucleoside, at least one 2'-OMe nucleoside, and at least one 2'-deoxynucleoside. In certain embodiments, the 3'-wing of a gapmer comprises at least one LNA nucleoside, at least one 2'-OMe nucleoside, and at least one 2'-deoxynucleoside.
• the gap of a gapmer consists of 6 to 20 linked nucleosides. In certain embodiments, the gap of a gapmer consists of 6 to 15 linked nucleosides. In certain embodiments, the gap of a gapmer consists of 6 to 12 linked nucleosides. In certain embodiments, the gap of a gapmer consists of 6 to
• the gap of a gapmer consists of 6 to 9 linked nucleosides. In certain embodiments, the gap of a gapmer consists of 6 to 8 linked nucleosides. In certain embodiments, the gap of a gapmer consists of 6 or 7 linked nucleosides. In certain embodiments, the gap of a gapmer consists of 7 to 10 linked nucleosides. In certain embodiments, the gap of a gapmer consists of 7 to 9 linked nucleosides. In certain embodiments, the gap of a gapmer consists of 7 or 8 linked nucleosides. In certain embodiments, the gap of a gapmer consists of 8 to 10 linked nucleosides.
• the gap of a gapmer consists of 8 or 9 linked nucleosides. In certain embodiments, the gap of a gapmer consists of 6 linked nucleosides. In certain embodiments, the gap of a gapmer consists of 7 linked nucleosides. In certain embodiments, the gap of a gapmer consists of 8 linked nucleosides. In certain embodiments, the gap of a gapmer consists of 9 linked nucleosides. In certain embodiments, the gap of a gapmer consists of 10 linked nucleosides. In certain embodiments, the gap of a gapmer consists of 11 linked nucleosides. In certain embodiments, the gap of a gapmer consists of 12 linked nucleosides.
• each nucleoside of the gap of a gapmer is a 2'-deoxynucleoside.
• the gap comprises one or more modified nucleosides.
• each nucleoside of the gap of a gapmer is a 2'-deoxynucleoside or is a modified nucleoside that is "DNA-like.”
• DNA-like means that the nucleoside has similar characteristics to DNA, such that a duplex comprising the gapmer and an RNA molecule is capable of activating RNase H. For example, under certain conditions, 2'-(ara)-F have been shown to support RNase H activation, and thus is DNA-like.
• one or more nucleosides of the gap of a gapmer is not a 2'-deoxynucleoside and is not DNA- like. In certain such embodiments, the gapmer nonetheless supports RNase H activation (e.g., by virtue of the number or placement of the non-DNA nucleosides).
• gaps comprise a stretch of unmodified 2'-deoxynucleoside interrupted by one or more modified nucleosides, thus resulting in three sub-regions (two stretches of one or more - deoxynucleosides and a stretch of one or more interrupting modified nucleosides).
• no stretch of unmodified 2'-deoxynucleosides is longer than 5, 6, or 7 nucleosides.
• such short stretches is achieved by using short gap regions.
• short stretches are achieved by interrupting a longer gap region.
• the gap comprises one or more modified nucleosides.
• the gap comprises one or more modified nucleosides selected from among cEt, FHNA, LNA, and 2-thio-thymidine. In certain embodiments, the gap comprises one modified nucleoside. In certain embodiments, the gap comprises a 5 '-substituted sugar moiety selected from among 5'-Me, and 5'-(R)-MQ. In certain embodiments, the gap comprises two modified nucleosides. In certain embodiments, the gap comprises three modified nucleosides. In certain embodiments, the gap comprises four modified nucleosides. In certain embodiments, the gap comprises two or more modified nucleosides and each modified nucleoside is the same. In certain embodiments, the gap comprises two or more modified nucleosides and each modified nucleoside is different.
• the gap comprises one or more modified linkages. In certain embodiments, the gap comprises one or more methyl phosphonate linkages. In certain embodiments the gap comprises two or more modified linkages. In certain embodiments, the gap comprises one or more modified linkages and one or more modified nucleosides. In certain embodiments, the gap comprises one modified linkage and one modified nucleoside. In certain embodiments, the gap comprises two modified linkages and two or more modified nucleosides.
• oligonucleotides comprise modified internucleoside linkages arranged along the oligonucleotide or region thereof in a defined pattern or modified internucleoside linkage motif. In certain embodiments, oligonucleotides comprise a region having an alternating internucleoside linkage motif. In certain embodiments, oligonucleotides of the present disclosure comprise a region of uniformly modified internucleoside linkages. In certain such embodiments, the oligonucleotide comprises a region that is uniformly linked by phosphorothioate internucleoside linkages. In certain embodiments, the oligonucleotide is uniformly linked by phosphorothioate internucleoside linkages.
• each internucleoside linkage of the oligonucleotide is selected from phosphodiester and phosphorothioate. In certain embodiments, each internucleoside linkage of the oligonucleotide is selected from phosphodiester and phosphorothioate and at least one internucleoside linkage is phosphorothioate.
• the oligonucleotide comprises at least 6 phosphorothioate internucleoside linkages. In certain embodiments, the oligonucleotide comprises at least 7 phosphorothioate internucleoside linkages. In certain embodiments, the oligonucleotide comprises at least 8 phosphorothioate internucleoside linkages. In certain embodiments, the oligonucleotide comprises at least 9 phosphorothioate internucleoside linkages. In certain embodiments, the oligonucleotide comprises at least 10 phosphorothioate internucleoside linkages.
• the oligonucleotide comprises at least 11 phosphorothioate internucleoside linkages. In certain embodiments, the oligonucleotide comprises at least 12 phosphorothioate internucleoside linkages. In certain embodiments, the oligonucleotide comprises at least 13 phosphorothioate internucleoside linkages. In certain embodiments, the oligonucleotide comprises at least 14 phosphorothioate internucleoside linkages.
• the oligonucleotide comprises at least one block of at least 6 consecutive phosphorothioate internucleoside linkages. In certain embodiments, the oligonucleotide comprises at least one block of at least 7 consecutive phosphorothioate internucleoside linkages. In certain embodiments, the oligonucleotide comprises at least one block of at least 8 consecutive phosphorothioate internucleoside linkages. In certain embodiments, the oligonucleotide comprises at least one block of at least 9 consecutive phosphorothioate internucleoside linkages.
• the oligonucleotide comprises at least one block of at least 10 consecutive phosphorothioate internucleoside linkages. In certain embodiments, the oligonucleotide comprises at least block of at least one 12 consecutive phosphorothioate internucleoside linkages. In certain such embodiments, at least one such block is located at the 3' end of the oligonucleotide. In certain such embodiments, at least one such block is located within 3 nucleosides of the 3' end of the oligonucleotide. In certain embodiments, the oligonucleotide comprises less than 15 phosphorothioate internucleoside linkages.
• the oligonucleotide comprises less than 14 phosphorothioate internucleoside linkages. In certain embodiments, the oligonucleotide comprises less than 13 phosphorothioate internucleoside linkages. In certain embodiments, the oligonucleotide comprises less than 12 phosphorothioate internucleoside linkages. In certain embodiments, the oligonucleotide comprises less than 11 phosphorothioate internucleoside linkages. In certain embodiments, the oligonucleotide comprises less than 10 phosphorothioate internucleoside linkages.
• the oligonucleotide comprises less than 9 phosphorothioate internucleoside linkages. In certain embodiments, the oligonucleotide comprises less than 8 phosphorothioate internucleoside linkages. In certain embodiments, the oligonucleotide comprises less than 7 phosphorothioate internucleoside linkages. In certain embodiments, the oligonucleotide comprises less than 6 phosphorothioate internucleoside linkages. In certain embodiments, the oligonucleotide comprises less than 5 phosphorothioate internucleoside linkages.
• oligonucleotides comprise chemical modifications to nucleobases arranged along the oligonucleotide or region thereof in a defined pattern or nucleobases modification motif.
• nucleobase modifications are arranged in a gapped motif.
• nucleobase modifications are arranged in an alternating motif.
• each nucleobase is modified.
• none of the nucleobases is chemically modified.
• oligonucleotides comprise a block of modified nucleobases.
• the block is at the 3 '-end of the oligonucleotide.
• the block is within 3 nucleotides of the 3'-end of the oligonucleotide.
• the block is at the 5'-end of the oligonucleotide.
• the block is within 3 nucleotides of the 5 '-end of the oligonucleotide.
• nucleobase modifications are a function of the natural base at a particular position of an oligonucleotide.
• each purine or each pyrimidine in an oligonucleotide is modified.
• each adenine is modified.
• each guanine is modified.
• each thymine is modified.
• each cytosine is modified.
• each uracil is modified.
• some, all, or none of the cytosine moieties in an oligonucleotide are 5- methyl cytosine moieties.
• 5-methyl cytosine is not a "modified nucleobase.”
• unmodified nucleobases include both cytosine residues having a 5-methyl and those lacking a 5 methyl.
• the methylation state of all or some cytosine nucleobases is specified.
• chemical modifications to nucleobases comprise attachment of certain conjugate groups to nucleobases.
• each purine or each pyrimidine in an oligonucleotide may be optionally modified to comprise a conjugate group.
• oligonucleotides of any of a variety of ranges of lengths.
• oligonucleotides consist of X to Y linked nucleosides, where X represents the fewest number of nucleosides in the range and Y represents the largest number of nucleosides in the range.
• X and Y are each independently selected from 8, 9, 10, 11, 12, 13,
• oligonucleotide may consist of 8 to 9, 8 to 10, 8 to 11, 8 to 12, 8 to 13, 8 to 14, 8 to 15, 8 to 16, 8 to 17, 8 to 18, 8 to 19, 8 to 20, 8 to 21, 8 to 22, 8 to 23, 8 to 24, 8 to 25, 8 to 26, 8 to 27, 8 to 28, 8 to 29, 8 to 30, 9 to 10,
• an oligonucleotide comprising 8-30 nucleosides excludes oligonucleotides having 31 nucleosides, but, unless otherwise indicated, such an oligonucleotide may further comprise, for example one or more conjugate groups, terminal groups, or other substituents.
• an oligonucleotide is described by an overall length range and by regions having specified lengths, and where the sum of specified lengths of the regions is less than the upper limit of the overall length range, the oligonucleotide may have additional nucleosides, beyond those of the specified regions, provided that the total number of nucleosides does not exceed the upper limit of the overall length range.
• the chemical structural features of antisense oligonucleotides are characterized by their sugar motif, internucleoside linkage motif, nucleobase modification motif and overall length. In certain embodiments, such parameters are each independent of one another. Thus, each internucleoside linkage of an oligonucleotide having a gapmer sugar motif may be modified or unmodified and may or may not follow the gapmer modification pattern of the sugar modifications. Thus, the internucleoside linkages within the wing regions of a sugar-gapmer may be the same or different from one another and may be the same or different from the internucleoside linkages of the gap region.
• sugar-gapmer oligonucleotides may comprise one or more modified nucleobase independent of the gapmer pattern of the sugar modifications.
• modified nucleobase independent of the gapmer pattern of the sugar modifications.
• One of skill in the art will appreciate that such motifs may be combined to create a variety of oligonucleotides.
• the selection of internucleoside linkage and nucleoside modification are not independent of one another. i. Certain Sequences and Targets
• the invention provides antisense oligonucleotides having a sequence complementary to a target nucleic acid.
• antisense compounds are capable of hybridizing to a target nucleic acid, resulting in at least one antisense activity.
• antisense compounds specifically hybridize to one or more target nucleic acid.
• a specifically hybridizing antisense compound has a nucleobase sequence comprising a region having sufficient complementarity to a target nucleic acid to allow hybridization and result in antisense activity and insufficient complementarity to any non-target so as to avoid or reduce non-specific hybridization to non-target nucleic acid sequences under conditions in which specific hybridization is desired (e.g., under physiological conditions for in vivo or therapeutic uses, and under conditions in which assays are performed in the case of in vitro assays).
• oligonucleotides are selective between a target and non-target, even though both target and non-target comprise the target sequence. In such embodiments, selectivity may result from relative accessibility of the target region of one nucleic acid molecule compared to the other.
• the present disclosure provides antisense compounds comprising oligonucleotides that are fully complementary to the target nucleic acid over the entire length of the oligonucleotide. In certain embodiments, oligonucleotides are 99% complementary to the target nucleic acid. In certain embodiments, oligonucleotides are 95% complementary to the target nucleic acid. In certain embodiments, such oligonucleotides are 90% complementary to the target nucleic acid.
• such oligonucleotides are 85% complementary to the target nucleic acid. In certain embodiments, such oligonucleotides are 80% complementary to the target nucleic acid. In certain embodiments, an antisense compound comprises a region that is fully complementary to a target nucleic acid and is at least 80% complementary to the target nucleic acid over the entire length of the oligonucleotide. In certain such embodiments, the region of full complementarity is from 6 to 14 nucleobases in length.
• oligonucleotides comprise a hybridizing region and a terminal region.
• the hybridizing region consists of 12-30 linked nucleosides and is fully complementary to the target nucleic acid.
• the hybridizing region includes one mismatch relative to the target nucleic acid.
• the hybridizing region includes two mismatches relative to the target nucleic acid.
• the hybridizing region includes three mismatches relative to the target nucleic acid.
• the terminal region consists of 1-4 terminal nucleosides.
• the terminal nucleosides are at the 3' end. In certain embodiments, one or more of the terminal nucleosides are not complementary to the target nucleic acid.
• Antisense mechanisms include any mechanism involving the hybridization of an oligonucleotide with target nucleic acid, wherein the hybridization results in a biological effect. In certain embodiments, such hybridization results in either target nucleic acid degradation or occupancy with concomitant inhibition or stimulation of the cellular machinery involving, for example, translation, transcription, or splicing of the target nucleic acid.
• RNase H is a cellular endonuclease which cleaves the RNA strand of an RNA:DNA duplex. It is known in the art that single-stranded antisense compounds which are "DNA-like" elicit RNase H activity in mammalian cells. Activation of RNase H, therefore, results in cleavage of the RNA target, thereby greatly enhancing the efficiency of DNA-like oligonucleotide-mediated inhibition of gene expression.
• a conjugate group comprises a cleavable moiety. In certain embodiments, a conjugate group comprises one or more cleavable bond. In certain embodiments, a conjugate group comprises a linker. In certain embodiments, a linker comprises a protein binding moiety. In certain embodiments, a conjugate group comprises a cell-targeting moiety (also referred to as a cell-targeting group). In certain embodiments a cell-targeting moiety comprises a branching group. In certain embodiments, a cell- targeting moiety comprises one or more tethers. In certain embodiments, a cell-targeting moiety comprises a carbohydrate or carbohydrate cluster.
• a cleavable moiety is a cleavable bond. In certain embodiments, a cleavable moiety comprises a cleavable bond. In certain embodiments, the conjugate group comprises a cleavable moiety. In certain such embodiments, the cleavable moiety attaches to the antisense
• the cleavable moiety attaches directly to the cell-targeting moiety. In certain such embodiments, the cleavable moiety attaches to the conjugate linker. In certain embodiments, the cleavable moiety comprises a phosphate or phosphodiester. In certain embodiments, the cleavable moiety is a cleavable nucleoside or nucleoside analog. In certain embodiments, the nucleoside or nucleoside analog comprises an optionally protected heterocyclic base selected from a purine, substituted purine, pyrimidine or substituted pyrimidine.
• the cleavable moiety is a nucleoside comprising an optionally protected heterocyclic base selected from uracil, thymine, cytosine, 4-N- benzoylcytosine, 5-methylcytosine, 4-N-benzoyl-5-methylcytosine, adenine, 6-N-benzoyladenine, guanine and 2-N-isobutyrylguanine.
• the cleavable moiety is 2'-deoxy nucleoside that is attached to the 3' position of the antisense oligonucleotide by a phosphodiester linkage and is attached to the linker by a phosphodiester or phosphorothioate linkage.
• the cleavable moiety is 2'- deoxy adenosine that is attached to the 3' position of the antisense oligonucleotide by a phosphodiester linkage and is attached to the linker by a phosphodiester or phosphorothioate linkage.
• the cleavable moiety is 2'-deoxy adenosine that is attached to the 3' position of the antisense oligonucleotide by a phosphodiester linkage and is attached to the linker by a phosphodiester linkage.
• the cleavable moiety is attached to the 3' position of the antisense oligonucleotide. In certain embodiments, the cleavable moiety is attached to the 5' position of the antisense oligonucleotide. In certain embodiments, the cleavable moiety is attached to a 2' position of the antisense oligonucleotide. In certain embodiments, the cleavable moiety is attached to the antisense oligonucleotide by a phosphodiester linkage. In certain embodiments, the cleavable moiety is attached to the linker by either a phosphodiester or a phosphorothioate linkage. In certain embodiments, the cleavable moiety is attached to the linker by a phosphodiester linkage. In certain embodiments, the conjugate group does not include a cleavable moiety.
• the cleavable moiety is cleaved after the complex has been administered to an animal only after being internalized by a targeted cell. Inside the cell the cleavable moiety is cleaved thereby releasing the active antisense oligonucleotide. While not wanting to be bound by theory it is believed that the cleavable moiety is cleaved by one or more nucleases within the cell. In certain embodiments, the one or more nucleases cleave the phosphodiester linkage between the cleavable moiety and the linker. In certain embodiments, the cleavable moiety has a structure selected from among the following:
• each of Bx, Bxi, Bx 2 , and Bx 3 is independently a heterocyclic base moiety.
• the cleavable moiety has a structure selected from amon the following:
• the conjugate groups comprise a linker.
• the linker is covalently bound to the cleavable moiety.
• the linker is covalently bound to the antisense oligonucleotide.
• the linker is covalently bound to a cell- targeting moiety.
• the linker further comprises a covalent attachment to a solid support.
• the linker further comprises a covalent attachment to a protein binding moiety.
• the linker further comprises a covalent attachment to a solid support and further comprises a covalent attachment to a protein binding moiety.
• the linker includes multiple positions for attachment of tethered ligands. In certain embodiments, the linker includes multiple positions for attachment of tethered ligands and is not attached to a branching group. In certain embodiments, the linker further comprises one or more cleavable bond. In certain embodiments, the conjugate group does not include a linker.
• the linker includes at least a linear group comprising groups selected from alkyl, amide, disulfide, polyethylene glycol, ether, thioether (-S-) and hydroxylamino (-O-N(H)-) groups.
• the linear group comprises groups selected from alkyl, amide and ether groups.
• the linear group comprises groups selected from alkyl and ether groups.
• the linear group comprises at least one phosphorus linking group.
• the linear group comprises at least one phosphodiester group.
• the linear group includes at least one neutral linking group.
• the linear group is covalently attached to the cell- targeting moiety and the cleavable moiety.
• the linear group is covalently attached to the cell-targeting moiety and the antisense oligonucleotide. In certain embodiments, the linear group is covalently attached to the cell-targeting moiety, the cleavable moiety and a solid support. In certain embodiments, the linear group is covalently attached to the cell-targeting moiety, the cleavable moiety, a solid support and a protein binding moiety. In certain embodiments, the linear group includes one or more cleavable bond.
• the linker includes the linear group covalently attached to a scaffold group.
• the scaffold includes a branched aliphatic group comprising groups selected from alkyl, amide, disulfide, polyethylene glycol, ether, thioether and hydroxylamino groups.
• the scaffold includes a branched aliphatic group comprising groups selected from alkyl, amide and ether groups.
• the scaffold includes at least one mono or polycyclic ring system.
• the scaffold includes at least two mono or polycyclic ring systems.
• the linear group is covalently attached to the scaffold group and the scaffold group is covalently attached to the cleavable moiety and the linker.
• the linear group is covalently attached to the scaffold group and the scaffold group is covalently attached to the cleavable moiety, the linker and a solid support. In certain embodiments, the linear group is covalently attached to the scaffold group and the scaffold group is covalently attached to the cleavable moiety, the linker and a protein binding moiety. In certain embodiments, the linear group is covalently attached to the scaffold group and the scaffold group is covalently attached to the cleavable moiety, the linker, a protein binding moiety and a solid support. In certain embodiments, the scaffold group includes one or more cleavable bond.
• the linker includes a protein binding moiety.
• the protein binding moiety is a lipid such as for example including but not limited to cholesterol, cholic acid, adamantane acetic acid, 1-pyrene butyric acid, dihydrotestosterone, l,3-Bis-0(hexadecyl)glycerol, geranyloxyhexyl group, hexadecylglycerol, borneol, menthol, 1,3-propanediol, heptadecyl group, palmitic acid, myristic acid, 03-(oleoyl)lithocholic acid, 03-(oleoyl)cholenic acid, dimethoxytrityl, or phenoxazine), a vitamin (e.g., folate, vitamin A, vitamin E, biotin, pyridoxal), a peptide, a carbohydrate (e.g., monos
• a linker has a structure selected from among:
• n is, independently, from 1 to 20; and p is from
• a linker has a structure selected from among:
• n is from 1 to 20.
• each L is, independently, a phosphorus linking group or a neutral linking group; and each n is, independently, from 1 to 20.
• a linker has a structure selected from among:
• n is from 1 to 20.
• a linker has a structure selected from among
• a linker has a structure selected from among
• the conjugate linker has the structure:
• the conjugate linker has the structure:
• a linker has a structure selected from among:
• a linker has a structure selected from among:
• conjugate groups comprise cell-targeting moieties. Certain such cell-targeting moieties increase cellular uptake of antisense compounds.
• cell- targeting moieties comprise a branching group, one or more tether, and one or more ligand. In certain embodiments, cell-targeting moieties comprise a branching group, one or more tether, one or more ligand and one or more cleavable bond.
• the conjugate groups comprise a targeting moiety comprising a branching group and at least two tethered ligands.
• the branching group attaches the conjugate linker.
• the branching group attaches the cleavable moiety.
• the branching group attaches the antisense oligonucleotide.
• the branching group is covalently attached to the linker and each of the tethered ligands.
• the branching group comprises a branched aliphatic group comprising groups selected from alkyl, amide, disulfide, polyethylene glycol, ether, thioether and hydroxylamino groups.
• the branching group comprises groups selected from alkyl, amide and ether groups. In certain embodiments, the branching group comprises groups selected from alkyl and ether groups. In certain embodiments, the branching group comprises a mono or polycvclic ring system. In certain embodiments, the branching group comprises one or more cleavable bond. In certain embodiments, the conjugate group does not include a branching group. In certain embodiments, a branching group has a structure selected from among:
• n is, independently, from 1 to 20;
• j is from 1 to 3;
• n 2 to 6.
• a branching group has a structure selected from among:
• n is, independently, from 1 to 20;
• n 2 to 6.
• a branching group has a structure selected from among:
• each n is, independently, from 1 to 20.
• a branching group has a structure selected from
• a branching group has a structure selected from
• each n is, independently, from 1 to 20.
• a branching group has a structure selected from
• a branching group has a structure selected from among:
• a branching group has a structure selected from among:
• conjugate groups comprise one or more tethers covalently attached to the branching group. In certain embodiments, conjugate groups comprise one or more tethers covalently attached to the linking group. In certain embodiments, each tether is a linear aliphatic group comprising one or more groups selected from alkyl, ether, thioether, disulfide, amide and polyethylene glycol groups in any combination. In certain embodiments, each tether is a linear aliphatic group comprising one or more groups selected from alkyl, substituted alkyl, ether, thioether, disulfide, amide, phosphodiester and polyethylene glycol groups in any combination.
• each tether is a linear aliphatic group comprising one or more groups selected from alkyl, ether and amide groups in any combination. In certain embodiments, each tether is a linear aliphatic group comprising one or more groups selected from alkyl, substituted alkyl, phosphodiester, ether and amide groups in any combination. In certain embodiments, each tether is a linear aliphatic group comprising one or more groups selected from alkyl and phosphodiester in any combination. In certain embodiments, each tether comprises at least one phosphorus linking group or neutral linking group.
• the tether includes one or more cleavable bond. In certain embodiments, the tether is attached to the branching group through either an amide or an ether group. In certain
• the tether is attached to the branching group through a phosphodiester group. In certain embodiments, the tether is attached to the branching group through a phosphorus linking group or neutral linking group. In certain embodiments, the tether is attached to the branching group through an ether group. In certain embodiments, the tether is attached to the ligand through either an amide or an ether group. In certain embodiments, the tether is attached to the ligand through an ether group. In certain embodiments, the tether is attached to the ligand through either an amide or an ether group. In certain embodiments, the tether is attached to the ligand through an ether group.
• each tether comprises from about 8 to about 20 atoms in chain length between the ligand and the branching group. In certain embodiments, each tether group comprises from about 10 to about 18 atoms in chain length between the ligand and the branching group. In certain embodiments, each tether group comprises about 13 atoms in chain length.
• a tether has a structure selected from among:
• n is, independently, from 1 to 20;
• each p is from 1 to about 6.
• a tether has a structure selected from among: In certain embodiments, a tether has a structure selected from among: wherein each n is, independently, from 1 to 20.
• a tether has a structure selected from among:
• L is either a phosphorus linking group or a neutral linking group
• Z 2 is H, C1-C6 alkyl or substituted C1-C6 alky
• R2 is H, C1-C6 alkyl or substituted C1-C6 alky
• each mi is, independently, from 0 to 20 wherein at least one mi is greater than 0 for each tether.
• a tether has a structure selected from among:
• a tether has a structure selected from among:
• Z 2 is H or CH 3 ;
• each mi is, independently, from 0 to 20 wherein at least one mi is greater than 0 for each tether.
• a tether has a structure selected from among: ; wherein each n is independently, 0, 1, 2, 3, 4, 5, 6, or 7.
• a tether comprises a phosphorus linking group. In certain embodiments, a tether does not comprise any amide bonds. In certain embodiments, a tether comprises a phosphorus linking group and does not comprise any amide bonds.
• each ligand is covalently attached to a tether.
• each ligand is selected to have an affinity for at least one type of receptor on a target cell.
• ligands are selected that have an affinity for at least one type of receptor on the surface of a mammalian liver cell.
• ligands are selected that have an affinity for the hepatic asialoglycoprotein receptor (ASGP-R).
• ASGP-R hepatic asialoglycoprotein receptor
• each ligand is a carbohydrate.
• each ligand is, independently selected from galactose, N-acetyl galactoseamine, mannose, glucose, glucosamone and fucose. In certain embodiments, each ligand is N-acetyl galactoseamine (GalNAc). In certain embodiments, the targeting moiety comprises 2 to 6 ligands. In certain embodiments, the targeting moiety comprises 3 ligands. In certain embodiments, the targeting moiety comprises 3 N-acetyl galactoseamine ligands.
• the ligand is a carbohydrate, carbohydrate derivative, modified carbohydrate, multivalent carbohydrate cluster, polysaccharide, modified polysaccharide, or polysaccharide derivative. In certain embodiments, the ligand is an amino sugar or a thio sugar.
• amino sugars may be selected from any number of compounds known in the art, for example glucosamine, sialic acid, a-D- galactosamine, N-Acetylgalactosamine, 2-acetamido-2-deoxy-D-galactopyranose (GalNAc), 2-Amino-3-0- [( ?)-l-carboxyethyl]-2-deoxy-P-D-glucopyranose ( ⁇ -muramic acid), 2-Deoxy-2-methylamino-L- glucopyranose, 4,6-Dideoxy-4-formamido-2,3-di-0-methyl-D-mannopyranose, 2-Deoxy-2-sulfoamino-D- glucopyranose and , ⁇ -sulfo-D-glucosamine, and N-Glycoloyl- -neuraminic acid.
• glucosamine sialic acid
• a-D- galactosamine N-Acetyl
• thio sugars may be selected from the group consisting of 5-Thio- -D-glucopyranose, Methyl 2,3,4-tri-0-acetyl-l-thio-6- O-trityl- -D-glucopyranoside, 4-Thio- -D-galactopyranose, and ethyl 3,4,6,7-tetra-Oacetyl-2-deoxy-l,5- dithio-a-D-g i/co-heptopyranoside.
• GalNac or “Gal-NAc” refers to 2-(Acetylamino)-2-deoxy-D- galactopyranose, commonly referred to in the literature as N-acetyl galactosamine.
• N-acetyl galactosamine refers to 2-(Acetylamino)-2-deoxy-D-galactop5Tanose.
• GalNac or “Gal-NAc” refers to 2-(Acetylamino)-2-deoxy-D-galactopyranose.
• GalNac or “Gal-NAc” refers to 2-(Acetylamino)-2-deoxy-D-galactopyranose, which includes both the ⁇ - form: 2-(Acetylamino)-2-deoxy- -D-galactopyranose and a-form: 2-(Acetylamino)-2-deoxy-D- galactopyranose.
• both the ⁇ -form: 2-(Acetylarnino)-2-deoxy-P-D-galactopyranose and a-form: 2-(Acetylamino)-2-deoxy-D-galactopyranose may be used interchangeably.
• these structures are intended to include the other form as well.
• this structure is intended to include the other form as well.
• the ⁇ -form 2-(Acetylamino)-2-deoxy-D-galactopyranose is the preferred embodiment.
• one or more ligand has a structure selected from amonj In certain embodiments one or more ligand has a structure selected from among:
• conjugate groups comprise the structural features above. In certain such embodiments, conjugate groups have the following structure:
• conjugate groups have the following structure:
• conjugate groups have the following structure:
• n is, independently, from 1 to 20;
• Z is H or a linked solid support
• Q is an antisense compound
• X is 0 or S
• Bx is a heterocyclic base moiety.
• conjugate groups have the following structure:
• conjugate groups have the following structure: In certain such embodiments, conjugate groups have the following structure:
• conjugate groups have the following structure: In certain such embodiments, conjugate groups have the following structure:
• conjugate groups have the following structure: In certain such embodiments, conjugate groups have the following structure:
• conjugates do not comprise a pyrrolidine.
• conjugate groups have the following structure:
• conjugate groups have the following structure: In certain such embodiments, conjugate groups have the following structure:
• conjugate groups have the following structure:
• conjugate groups have the following structure: In certain such embodiments, conjugate groups have the following structure:
• conjugate groups have the following structure:
• conjugate groups have the following structure: In certain such embodiments, conjugate groups have the following structure:
• conjugate groups have the following structure:
• the cell-targeting moiety of the conjugate group has the following structure:
• X is a substituted or unsubstituted tether of six to eleven consecutively bonded atoms.
• the cell-targeting moiety of the conjugate group has the following structure:
• X is a substituted or unsubstituted tether of ten consecutively bonded atoms.
• the cell-targeting moiety of the conjugate group has the following
• the cell-targeting moiety of the conjugate group has the following structure:
• Y and Z are independently selected from a C 1 -C 12 substituted or unsubstituted alkyl, alkenyl, or alkynyl group, or a group comprising an ether, a ketone, an amide, an ester, a carbamate, an amine, a piperidine, a phosphate, a phosphodiester, a phosphorothioate, a triazole, a pyrrolidine, a disulfide, or a thioether.
• the cell-targeting moiety of the conjugate group has the following structure:
• Y and Z are independently selected from a C 1 -C 12 substituted or unsubstituted alkyl group, or a group comprising exactly one ether or exactly two ethers, an amide, an amine, a piperidine, a phosphate, phosphodiester, or a phosphorothioate.
• the cell-targeting moiety of the conjugate group has the following structure:
• Y and Z are independently selected from a Ci-Ci 2 substituted or unsubstituted alkyl group.
• the cell-targeting moiety of the conjugate group has the following structure:
• n and n are independently selected from 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, and 12.
• the cell-targeting moiety of the conjugate group has the following structure:
• the cell-targeting moiety of the conjugate group has the following structure:
• X is a substituted or unsubstituted tether of four to thirteen consecutively bonded atoms, and wherein X does not comprise an ether group.
• the cell-targeting moiety of the conjugate group has the following structure:
• X is a substituted or unsubstituted tether of eight consecutively bonded atoms, and wherein X does not comprise an ether group.
• the cell-targeting moiety of the conjugate group has the following structure:
• X is a substituted or unsubstituted tether of four to thirteen consecutively bonded atoms, and wherein the tether comprises exactly one amide bond, and wherein X does not comprise an ether group.
• the cell-targeting moiety of the conjugate group has the following structure:
• X is a substituted or unsubstituted tether of four to thirteen consecutively bonded atoms and wherein the tether consists of an amide bond and a substituted or unsubstituted C 2 -Cn alkyl group.
• the cell-targeting moiety of the conjugate group has the following structure:
• Y is selected from a C1-C12 substituted or unsubstituted alkyl, alkenyl, or alkynyl group, or a group comprising an ether, a ketone, an amide, an ester, a carbamate, an amine, a piperidine, a phosphate, a phosphodiester, a phosphorothioate, a triazole, a pyrrolidine, a disulfide, or a thioether.
• the cell-targeting moiety of the conjugate group has the following structure:
• Y is selected from a C1-C12 substituted or unsubstituted alkyl group, or a group comprising an ether, an amine, a piperidine, a phosphate, a phosphodiester, or a phosphorothioate.
• the cell-targeting moiety of the conjugate group has the following structure:
• Y is selected from a C1-C12 substituted or unsubstituted alkyl group.
• the cell-targeting moiety of the conjugate group has the following structure:
• n 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12.
• the cell-targeting moiety of the conjugate group has the following structure:
• n 4, 5, 6, 7, or 8.
• conjugates do not comprise a pyrrolidine.
• conjugates are bound to a nucleoside of the antisense oligonucleotide at the 2', 3', of 5' position of the nucleoside.
• a conjugated antisense compound has the following structure:
• A is the antisense oligonucleotide
• each E is a tether
• each F is a ligand
• q is an integer between 1 and 5.
• a conjugated antisense compound has the following structure:
• A is the antisense oligonucleotide
• each E is a tether
• each F is a ligand
• q is an integer between 1 and 5.
• the conjugate linker comprises at least one cleavable bond.
• the branching group comprises at least one cleavable bond.
• each tether comprises at least one cleavable bond.
• the conjugates are bound to a nucleoside of the antisense oligonucleotide at the 2',
• a conjugated antisense compound has the following structure:
• A is the antisense oligonucleotide
• each E is a tether
• each F is a ligand
• q is an integer between 1 and 5.
• the conjugates are bound to a nucleoside of the antisense oligonucleotide at the V, 3', of 5' position of the nucleoside.
• a conjugated antisense compound has the following structure:
• A is the antisense oligonucleotide
• each E is a tether
• each F is a ligand
• q is an integer between 1 and 5.
• a conjugated antisense compound has the following structure:
• A is the antisense oligonucleotide
• each E is a tether
• each F is a ligand
• q is an integer between 1 and 5.
• a conjugated antisense compound has the following structure:
• A is the antisense oligonucleotide
• each E is a tether
• each F is a ligand
• q is an integer between 1 and 5.
• the conjugate linker comprises at least one cleavabie bond.
• each tether comprises at least one cleavabie bond.
• a conjugated antisense compound has a structure selected from among the following:
• a conjugated antisense compound has a structure selected from among the following:
• a conjugated antisense compound has a structure selected from among following:
• conjugated antisense compounds comprise an RNase H based oligonucleotide (such as a gapmer) or a splice modulating oligonucleotide (such as a fully modified oligonucleotide) and any conjugate group comprising at least one, two, or three GalNAc groups.
• a conjugated antisense compound comprises any conjugate group found in any of the following references: Lee, Carbohydr Res, 1978, 67, 509-514; Connolly et al., J Biol Chem, 1982, 257, 939-945; Pavia et al., bit J Pep Protein Res, 1983, 22, 539-548; Lee et al., Biochem, 1984, 23, 4255-4261; Lee et al., Gfycoconjugate J, 1987, 4, 317-328; Toyokuni et al., Tetrahedron Lett, 1990, 31, 2673-2676; Biessen et al., J Med Chem, 1995, 38, 1538-1546; Valentijn et al., Tetrahedron, 1997, 53, 759-770; Kim et al, Tetrahedron Lett, 1997, 38, 3487-3490; Lee et al., Bioconjug Chem, 1997, 8, 762-765; Kato
• WO2013/166121 WO2013/165816; U.S. Patents 4,751,219; 8,552,163; 6,908,903 7,262,177; 5,994,517 6,300,319; 8,106,022; 7,491,805; 7,491,805; 7,582,744; 8,137,695; 6,383,812; 6,525,031; 6,660,720 7,723,509; 8,541,548; 8,344,125; 8,313,772; 8,349,308; 8,450,467; 8,501,930; 8,158,601; 7,262,177 6,906,182; 6,620,916; 8,435,491 ; 8,404,862; 7,851,615; Published U.S. Patent Application Publications
• conjugated antisense compounds exhibit potent target RNA reduction in vivo.
• unconjugated antisense compounds accumulate in the kidney.
• conjugated antisense compounds accumulate in the liver.
• conjugated antisense compounds are well tolerated. Such properties render conjugated antisense compounds particularly useful for inhibition of many target RNAs, including, but not limited to those involved in metabolic, cardiovascular and other diseases, disorders or conditions.
• methods of treating such diseases, disorders or conditions by contacting liver tissues with the conjugated antisense compounds targeted to RNAs associated with such diseases, disorders or conditions.
• conjugated antisense compounds are more potent than unconjugated counterpart at a particular tissue concentration.
• the conjugate may allow the conjugated antisense compound to enter the cell more efficiently or to enter the cell more productively.
• conjugated antisense compounds may exhibit greater target reduction as compared to its unconjugated counterpart wherein both the conjugated antisense compound and its unconjugated counterpart are present in the tissue at the same concentrations.
• conjugated antisense compounds may exhibit greater target reduction as compared to its unconjugated counterpart wherein both the conjugated antisense compound and its unconjugated counterpart are present in the liver at the same concentrations.
• the conjugate groups described herein may further improve potency by increasing the affinity of the conjugated antisense compound for a particular type of cell or tissue. In certain embodiments, the conjugate groups described herein may further improve potency by increasing recognition of the conjugated antisense compound by one or more cell-surface receptors. . In certain embodiments, the conjugate groups described herein may further improve potency by facilitating endocytosis of the conjugated antisense compound. In certain embodiments, the cleavable moiety may further improve potency by allowing the conjugate to be cleaved from the antisense oligonucleotide after the conjugated antisense compound has entered the cell. Accordingly, in certain embodiments, conjugated antisense compounds can be administed at doses lower than would be necessary for unconjugated antisense oligonucleotides.
• Phosphorothioate linkages have been incorporated into antisense oligonucleotides previously. Such phosphorothioate linkages are resistant to nucleases and so improve stability of the oligonucleotide. Further, phosphorothioate linkages also bind certain proteins, which results in accumulation of antisense
• Oligonucleotides with fewer phosphorothioate linkages accumulate less in the liver and more in the kidney (see, for example, Geary, R., "Pharmacokinetic Properties of 2'-0-(2- Methoxyethyl)-Modified Oligonucleotide Analogs in Rats," Journal of Pharmacology and Experimental Therapeutics, Vol. 296, No.
• phosphodiester internucleoside linkages accumulate less in the liver and more in the kidney. When treating diseases in the liver, this is undesibable for several reasons (1) less drug is getting to the site of desired action (liver); (2) drug is escaping into the urine; and (3) the kidney is exposed to relatively high concentration of drug which can result in toxicities in the kidney. Thus, for liver diseases, phosphorothioate linkages provide important benefits. In certain embodiments, however, administration of oligonucleotides uniformly linked by phosphorothioate internucleoside linkages induces one or more proinflammatory reactions, (see for example: J Lab Clin Med. 1996 Sep;128(3):329-38.
• oligonucleotides wherein most of the internucleoside linkages comprise phosphorothioate internucleoside linkages induces one or more proinflammatory reactions.
• the degree of proinflammatory effect may depend on several variables (e.g. backbone modification, off-target effects, nucleobase modifications, and/or nucleoside modifications) see for example: Toxicologic Properties in Antisense a Drug Technology, Chapter 12, pages 342-351, Crooke, S.T., ed., 2008).
• the degree of proinflammatory effect may be mitigated by adjusting one or more variables. For example the degree of proinflammatory effect of a given oligonucleotide may be mitigated by replacing any number of phosphorothioate internucleoside linkages with phosphodiester internucleoside linkages and thereby reducing the total number of phosphorothioate internucleoside linkages.
• the number of phosphorothioate linkages may be reduced by replacing phosphorothioate linkages with phosphodiester linkages.
• the antisense compound having fewer phosphorothioate linkages and more phosphodiester linkages may induce less proinflammatory reactions or no proinflammatory reaction.
• the antisense compound having fewer phosphorothioate linkages and more phosphodiester linkages may induce fewer proinflammatory reactions
• the antisense compound having fewer phosphorothioate linkages and more phosphodiester linkages may not accumulate in the liver and may be less efficacious at the same or similar dose as compared to an antisense compound having more phosphorothioate linkages.
• conjugated antisense compounds accumulate more in the liver and less in the kidney than unconjugated counterparts, even when some of the phosporothioate linkages are replaced with less proinflammatory phosphodiester internucleoside linkages. In certain embodiments, conjugated antisense compounds accumulate more in the liver and are not excreted as much in the urine compared to its unonjugated counterparts, even when some of the phosporothioate linkages are replaced with less proinflammatory phosphodiester internucleoside linkages. In certain embodiments, the use of a conjugate allows one to design more potent and better tolerated antisense drugs.
• conjugated antisense compounds have larger therapeutic indexes than unconjugated counterparts. This allows the conjugated antisense compound to be administered at a higher absolute dose, because there is less risk of proinflammatory response and less risk of kidney toxicity. This higher dose, allows one to dose less frequently, since the clearance (metabolism) is expected to be similar. Further, because the compound is more potent, as described above, one can allow the concentration to go lower before the next dose without losing therapeutic activity, allowing for even longer periods between dosing. In certain embodiments, the inclusion of some phosphorothioate linkages remains desirable.
• terminal linkages are vulnerable to exonucleoases and so in certain embodiments, those linkages are phosphorothioate or other modified linkage.
• Internucleoside linkages linking two deoxynucleosides are vulnerable to endonucleases and so in certain embodiments those those linkages are phosphorothioate or other modified linkage.
• Internucleoside linkages between a modified nucleoside and a deoxynucleoside where the deoxynucleoside is on the 5' side of the linkage deoxynucleosides are vulnerable to endonucleases and so in certain embodiments those those linkages are phosphorothioate or other modified linkage.
• Internucleoside linkages between two modified nucleosides of certain types and between a deoxynucleoside and a modified nucleoside of certain typ where the modified nucleoside is at the 5' side of the linkage are sufficiently resistant to nuclease digestion, that the linkage can be phosphodiester.
• the antisense oligonucleotide of a conjugated antisense compound comprises fewer than 16 phosphorthioate linkages. In certain embodiments, the antisense oligonucleotide of a conjugated antisense compound comprises fewer than 15 phosphorthioate linkages. In certain
• the antisense oligonucleotide of a conjugated antisense compound comprises fewer than 14 phosphorthioate linkages. In certain embodiments, the antisense oligonucleotide of a conjugated antisense compound comprises fewer than 13 phosphorthioate linkages. In certain embodiments, the antisense oligonucleotide of a conjugated antisense compound comprises fewer than 12 phosphorthioate linkages. In certain embodiments, the antisense oligonucleotide of a conjugated antisense compound comprises fewer than 11 phosphorthioate linkages.
• the antisense oligonucleotide of a conjugated antisense compound comprises fewer than 10 phosphorthioate linkages. In certain embodiments, the antisense oligonucleotide of a conjugated antisense compound comprises fewer than 9 phosphorthioate linkages. In certain embodiments, the antisense oligonucleotide of a conjugated antisense compound comprises fewer than 8 phosphorthioate linkages.
• antisense compounds comprsing one or more conjugae group described herein has increased activity and/or potency and/or tolerability compared to a parent antisense compound lacking such one or more conjugate group. Accordingly, in certain embodiments, attachment of such conjugate groups to an oligonucleotide is desirable. Such conjugate groups may be attached at the 5'-, and/or 3'- end of an oligonucleotide. In certain instances, attachment at the 5'-end is synthetically desireable.
• oligonucleietides are synthesized by attachment of the 3' terminal nucleoside to a solid support and sequential coupling of nucleosides from 3' to 5' using techniques that are well known in the art. Accordingly if a conjugate group is desred at the 3'-terminus, one may (1) attach the conjugate group to the 3'-terminal nucleoside and attach that conjugated nucleoside to the solid support for subsequent preparation of the oligonucleotide or (2) attach the conjugate group to the 3 '-terminal nucleoside of a completed oligonucleotide after synthesis. Niether of these approaches is very efficient and thus both are costly.
• attachment of the conjugated nucleoside to the solid support is an inefficient process.
• attaching a conjugate group to the 5 '-terminal nucleoside is synthetically easier than attachment at the 3'-end.
• conjugated antisense compounds are administered to a subject.
• antisense compounds comprsing one or more conjugae group described herein has increased activity and/or potency and/or tolerability compared to a parent antisense compound lacking such one or more conjugate group. Without being bound by mechanism, it is believed that the conjugate group helps with distribution, delivery, and/or uptake into a target cell or tissue.
• the conjugate group once inside the target cell or tissue, it is desirable that all or part of the conjugate group to be cleaved to release the active oligonucleotide. In certain embodiments, it is not necessary that the entire conjugate group be cleaved from the oligonucleotide.
• a conjugated oligonucleotide was administered to mice and a number of different chemical species, each comprising a different portion of the conjugate group remaining on the oligonucleotide, were detected (Table 10a). This conjugated antisense compound demonstrated good potency (Table 10).
• such metabolite profile of multiple partial cleavage of the conjugate group does not interfere with activity/potency.
• a prodrug conjugated oligonucleotide
• a prodrug conjugated oligonucleotide
• multiple forms of the active compound may be necessary to determine relative amounts and activities for each one.
• regulatory review e.g., USFDA or counterpart
• it is desirable that such single active species be the antisense oligonucleotide lacking any portion of the conjugate group.
• conjugate groups at the 5'-end are more likely to result in complete metabolism of the conjugate group.
• endogenous enzymes responsible for metabolism at the 5' end are more active/efficient than the 3' counterparts.
• the specific conjugate groups are more amenable to metabolism to a single active species.
• certain conjugate groups are more amenable to metabolism to the oligonucleotide.
• oligomeric compounds of the present invention are antisense compounds.
• the oligomeric compound is complementary to a target nucleic acid.
• a target nucleic acid is an RNA.
• a target nucleic acid is a non-coding RNA.
• a target nucleic acid encodes a protein.
• a target nucleic acid is selected from a mRNA, a pre-mRNA, a microRNA, a non-coding RNA, including small non- coding RNA, and a promoter-directed RNA.
• oligomeric compounds are at least partially complementary to more than one target nucleic acid.
• oligomeric compounds of the present invention may be microRNA mimics, which typically bind to multiple targets.
• antisense compounds comprise a portion having a nucleobase sequence at least 70% complementary to the nucleobase sequence of a target nucleic acid. In certain embodiments, antisense compounds comprise a portion having a nucleobase sequence at least 80% complementary to the nucleobase sequence of a target nucleic acid. In certain embodiments, antisense compounds comprise a portion having a nucleobase sequence at least 90% complementary to the nucleobase sequence of a target nucleic acid. In certain embodiments, antisense compounds comprise a portion having a nucleobase sequence at least 95% complementary to the nucleobase sequence of a target nucleic acid.
• antisense compounds comprise a portion having a nucleobase sequence at least 98% complementary to the nucleobase sequence of a target nucleic acid. In certain embodiments, antisense compounds comprise a portion having a nucleobase sequence that is 100% complementary to the nucleobase sequence of a target nucleic acid. In certain embodiments, antisense compounds are at least 70%, 80%, 90%, 95%, 98%, or 100% complementary to the nucleobase sequence of a target nucleic acid over the entire length of the antisense compound. Antisense mechanisms include any mechanism involving the hybridization of an oligomeric compound with target nucleic acid, wherein the hybridization results in a biological effect.
• such hybridization results in either target nucleic acid degradation or occupancy with concomitant inhibition or stimulation of the cellular machinery involving, for example, translation, transcription, or polyadenylation of the target nucleic acid or of a nucleic acid with which the target nucleic acid may otherwise interact.
• RNase H is a cellular endonuclease which cleaves the RNA strand of an RNADNA duplex. It is known in the art that single-stranded antisense compounds which are "DNA-like" elicit RNase H activity in mammalian cells. Activation of RNase H, therefore, results in cleavage of the RNA target, thereby greatly enhancing the efficiency of DNA-like oligonucleotide-mediated inhibition of gene expression.
• Antisense mechanisms also include, without limitation RNAi mechanisms, which utilize the RISC pathway.
• RNAi mechanisms include, without limitation siRNA, ssRNA and microRNA mechanisms.
• Such mechanisms include creation of a microRNA mimic and/or an anti- microRNA.
• Antisense mechanisms also include, without limitation, mechanisms that hybridize or mimic non- coding RNA other than microRNA or mRNA.
• non-coding RNA includes, but is not limited to promoter-directed RNA and short and long RNA that effects transcription or translation of one or more nucleic acids.
• oligonucleotides comprising conjugates described herein are RNAi compounds. In certain embodiments, oligomeric oligonucleotides comprising conjugates described herein are ssRNA compounds. In certain embodiments, oligonucleotides comprising conjugates described herein are paired with a second oligomeric compound to form an siRNA. In certain such embodiments, the second oligomeric compound also comprises a conjugate. In certain embodiments, the second oligomeric compound is any modified or unmodified nucleic acid. In certain embodiments, the oligonucleotides comprising conjugates described herein is the antisense strand in an siRNA compound.
• the oligonucleotides comprising conjugates described herein is the sense strand in an siRNA compound.
• the conjugated oligomeric compound is double-stranded siRnA
• the conjugate may be on the sense strand, the antisense strand or both the sense strand and the antisense strand.
• conjugated antisense compounds target any nucleic acid.
• the target nucleic acid encodes a target protein that is clinically relevant. In such
• modulation of the target nucleic acid results in clinical benefit.
• Certain target nucleic acids include, but are not limited to, the target nucleic acids illustrated in Table 1.
• the targeting process usually includes determination of at least one target region, segment, or site within the target nucleic acid for the antisense interaction to occur such that the desired effect will result.
• a target region is a structurally defined region of the nucleic acid.
• a target region may encompass a 3' UTR, a 5' UTR, an ex on, an intron, a coding region, a translation initiation region, translation termination region, or other defined nucleic acid region or target segment.
• a target segment is at least about an 8-nucleobase portion of a target region to which a conjugated antisense compound is targeted.
• Target segments can include DNA or RNA sequences that comprise at least 8 consecutive nucleobases from the 5'-terminus of one of the target segments (the remaining nucleobases being a consecutive stretch of the same DNA or RNA beginning immediately upstream of the 5'-terminus of the target segment and continuing until the DNA or RNA comprises about 8 to about 30 nucleobases).
• Target segments are also represented by DNA or RNA sequences that comprise at least 8 consecutive nucleobases from the 3 '-terminus of one of the target segments (the remaining nucleobases being a consecutive stretch of the same DNA or RNA beginning immediately downstream of the 3'-terminus of the target segment and continuing until the DNA or RNA comprises about 8 to about 30 nucleobases).
• Target segments can also be represented by DNA or RNA sequences that comprise at least 8 consecutive nucleobases from an internal portion of the sequence of a target segment, and may extend in either or both directions until the conjugated antisense compound comprises about 8 to about 30 nucleobases.
• antisense compounds targeted to the nucleic acids listed in Table 1 can be modified as described herein.
• the antisense compounds can have a modified sugar moiety, an unmodified sugar moiety or a mixture of modified and unmodified sugar moieties as described herein.
• the antisense compounds can have a modified internucleoside linkage, an unmodified internucleoside linkage or a mixture of modified and unmodified internucleoside linkages as described herein.
• the antisense compounds can have a modified nucleobase, an unmodified nucleobase or a mixture of modified and unmodified nucleobases as described herein.
• the antisense compounds can have a motif as described herein.
• antisense compounds targeted to the nucleic acids listed in Table 1 can be conjugated as described herein.
• Protein tyrosine phosphatase IB is a member of a family of PTPs (Barford, et al., Science 1994. 263: 1397-1404) and is a cytosolic enzyme (Neel and Tonks, Curr. Opin. Cell Biol. 1997. 9: 193-204). PTPIB is expressed ubiquitously including tissues that are key regulators of insulin metabolism such as liver, muscle and fat (Goldstein, Receptor 1993. 3: 1-15), where it is the main PTP enzyme.
• PTPIB is considered to be a negative regulator of insulin signaling.
• PTPIB interacts with and dephosphorylates the insulin receptor, thus attenuating and potentially terminating the insulin signaling transduction (Goldstein et al., J. Biol. Chem. 2000. 275: 4383-4389).
• the physiological role of PTPIB in insulin signaling has been demonstrated in knockout mice models. Mice lacking the PTPIB gene were protected against insulin resistance and obesity (Elchebly et al., Science 1999. 283: 1544-1548).
• PTP1B- deficient mice had low adiposity, increased basal metabolic rate as well as total energy expenditure and were protected from diet-induced obesity.
• Insulin-stimulated glucose uptake was elevated in skeletal muscle, whereas adipose tissue was unaffected providing evidence that increased insulin sensitivity in PTP IB- deficient mice was tissue-specific (Klaman et al, Mol. Cell. Biol. 2000. 20: 5479-5489). These mice were phenotypically normal and were also resistant to diet-induced obesity, insulin resistance and had significantly lower triglyceride levels on a high-fat diet. Therefore, inhibition of PTPIB in patients suffering from Type II diabetes, metabolic syndrome, diabetic dyslipidemia, or related metabolic diseases would be beneficial.
• Antisense inhibition of PTPIB provides a unique advantage over traditional small molecule inhibitors in that antisense inhibitors do not rely on competitive binding of the compound to the protein and inhibit activity directly by reducing the expression of PTPIB.
• Antisense technology is emerging as an effective means for reducing the expression of certain gene products and may therefore prove to be uniquely useful in a number of therapeutic, diagnostic, and research applications for the modulation of PTP1B.
• conjugated antisense compounds are targeted to a PTP1B nucleic acid having the sequence of GENBANK® Accession No. NM 002827.2, incorporated herein as SEQ ID NO: 1 or GENBANK Accession No. NT 011362.9 truncated from nucleotides 14178000 to 14256000, incorporated herein as SEQ ID NO: 2.
• a conjugated antisense compound targeted to SEQ ID NO: 1 is at least 90%, at least 95%, or 100% complementary to SEQ ID NOs: 1 and/or 2.
• a conjugated antisense compound targeted to SEQ ID NO: 1 comprises an at least 8 consecutive nucleobase sequence of SEQ ID NO: 54. In certain embodiments, a conjugated antisense compound targeted to SEQ ID NO: 1 comprises a nucleobase sequence of SEQ ID NO: 54.
• a conjugated antisense compound targeted to SEQ ID NO: 1 comprises an at least 8 consecutive nucleobase sequence of SEQ ID NO: 55. In certain embodiments, a conjugated antisense compound targeted to SEQ ID NO: 1 comprises a nucleobase sequence of SEQ ID NO: 55. In certain embodiments, a conjugated antisense compound targeted to SEQ ID NO: 1 comprises an at least 8 consecutive nucleobase sequence of SEQ ID NO: 56. In certain embodiments, a conjugated antisense compound targeted to SEQ ID NO: 1 comprises a nucleobase sequence of SEQ ID NO: 56.
• a conjugated antisense compound targeted to SEQ ID NO: 1 comprises an at least 8 consecutive nucleobase sequence of SEQ ID NO: 57. In certain embodiments, a conjugated antisense compound targeted to SEQ ID NO: 1 comprises a nucleobase sequence of SEQ ID NO: 57.
• a compound comprises or consists of ISIS 142082 and a conjugate group.
• ISIS 142082 is a modified oligonucleotide having the formula: Aes Aes Aes Tes Ges Gds Tds Tds Tds Ads Tds Tds mCds mCds Ads Tes Ges Ges mCes mCe, wherein,
• A an adenine
• mC a 5'-methylcytosine
• G a guanine
• T a thymine
• e a 2'-0-methoxyethyl modified nucleoside
• d a 2'-deoxynucleoside
• s a phosphorothioate internucleoside linkage
• a compound comprises or consists of ISIS 404173 and a conjugate group.
• ISIS 404173 is a modified oligonucleotide having the formula: Aes Aes Tes Ges Ges Tds Tds Tds Ads Tds Tds mCds mCds Ads Tds Ges Ges mCes mCes Ae, wherein,
• A an adenine
• mC a 5'-methylcytosine
• G a guanine
• T a thymine
• e a 2'-0-methoxyethyl modified nucleoside
• d a 2'-deoxynucleoside
• s a phosphorothioate internucleoside linkage
• a compound comprises or consists of ISIS 409826 and a conjugate group.
• a compound comprises or consists of ISIS 446431 and a conjugate group.
• ISIS 446431 is a modified oligonucleotide having the formula: Aes Aes Tes Ges Gds Tds Tds Tds Ads Tds Tds mCds mCds Ads Tes Ges Ges mCe, wherein,
• A an adenine
• mC a 5'-methylcytosine
• G a guanine
• T a thymine
• e a 2'-0-methoxyethyl modified nucleoside
• d a 2'-deoxynucleoside
• s a phosphorothioate internucleoside linkage
• a compound comprises an antisense oligonucleotide disclosed in US 7,563,884 and WO 2007/131237, which is incorporated by reference in its entirety herein, and a conjugate group described herein.
• a compound comprises an antisense oligonucleotide having a nucleobase sequence of any of SEQ ID NOs 17-96 and 244-389 disclosed in US 7,563,884 and a conjugate group described herein.
• a compound comprises an antisense oligonucleotide having a nucleobase sequence of any of SEQ ID NOs 886-1552 of SEQ ID Nos WO 2007/131237 and a conjugate group described hereinJn certain embodiments, a compound comprises an antisense oligonucleotide disclosed in US 7,563,884 and WO 2007/131237, which is incorporated by reference in its entirety herein, and a conjugate group described herein. In certain embodiments, a compound comprises an antisense oligonucleotide having a nucleobase sequence of any of SEQ ID NOs disclosed in US 7,563,884 and WO 2007/131237 and a conjugate group described herein.
• PTP1B Therapeutic Indications In certain embodiments, provided herein are methods of treating an individual comprising administering one or more conjugated pharmaceutical compositions as described herein. In certain embodiments, the individual has metabolic related disease.
• conjugated compounds targeted to PTPIB have been shown to reduce the severity of physiological symptoms of metabolic related diseases, including metabolic syndrome, diabetes mellitus, insulin resistance, diabetic dyslipidemia, hypertriglyceridemia, obesity and weight gain.
• metabolic syndrome including metabolic syndrome, diabetes mellitus, insulin resistance, diabetic dyslipidemia, hypertriglyceridemia, obesity and weight gain.
• the conjugated compounds reduced blood glucose levels, e.g., the animals continued to experience symptoms, but the symptoms were less severe compared to untreated animals.
• the conjugated compounds appear to reduce the symptoms of diabetes; e.g., animals treated for a longer period of time experienced less severe symptoms than those administered the compounds for a shorter period of time.
• the conjugated compounds appear to inhibit weight gain; e.g., animals treated for a longer period of time experienced less severe symptoms than those administered the compounds for a shorter period of time. In other of the experiments, however, the conjugated compounds appear to inhibit hypertriglyceridemia; e.g., animals treated for a longer period of time experienced less severe symptoms than those administered the compounds for a shorter period of time.
• the ability of the conjugated compounds exemplified below to restore function therefore demonstrates that symptoms of the disease may be reversed by treatment with a compound as described herein.
• Diabetes mellitus is characterized by numerous physical and physiological symptoms. Any symptom known to one of skill in the art to be associated with Type 2 diabetes can be ameliorated or otherwise modulated as set forth above in the methods described above.
• the symptom is a physical symptom selected from the group consisting of increased glucose levels, increased weight gain, frequent urination, unusual thirst, extreme hunger, extreme fatigue, blurred vision, frequent infections, tingling or numbness at the extremities, dry and itchy skin, weight loss, slow-healing sores, and swollen gums.
• the symptom is a physiological symptom selected from the group consisting of increased insulin resistance, increased glucose levels, increased fat mass, decreased metabolic rate, decreased glucose clearance, decreased glucose tolerance, decreased insulin sensitivity, decreased hepatic insulin sensitivity, increased adipose tissue size and weight, increased body fat, and increased body weight.
• PTPIB binds to and serves as a substrate for the epidermal growth factor receptor (EGFR) (Liu and Chemoff, Biochem. J., 1997, 327, 139-145). Furthermore, in A431 human epidermoid carcinoma cells, PT1B was found to be inactivated by the presence of H2O2 generated by the addition of EGF. These studies indicate that PTPIB can be negatively regulated by the oxidation state of the cell, which is often deregulated during tumorigenesis (Lee et al., J. Biol. Chem., 1998, 273, 15366-15372).
• PTP1B has been shown to suppress transformation in NIH3T3 cells induced by the neu oncogene (Brown-Shimer et al., Cancer Res., 1992, 52, 478-482), as well as in rat 3Y1 fibroblasts induced by v-srk, v- src, and v-ras (Liu et al., Mol. Cell. Biol, 1998, 18, 250-259) and rat-1 fibroblasts induced by bcr-abl (LaMontagne et al., Proc. Natl. Acad. Sci. U. S. A., 1998, 95, 14094-14099).
• PTP1B promotes differentiation of 562 cells, a chronic myelogenous leukemia cell line, in a similar manner as does an inhibitor of the bcr-abl oncoprotein.
• the hy erproliferative disorder is cancer.
• provided herein are methods for ameliorating a symptom associated with cancer.
• provided is a method for reducing the rate of onset of a symptom associated with hyperproliferative disorders.
• provided is a method for reducing the rate of onset of a symptom associated with cancer.
• provided is a method for reducing the severity of a symptom associated with hyperproliferative disorders.
• provided is a method for reducing the severity of a symptom associated with cancer.
• the methods comprise administering to an individual in need thereof a therapeutically effective amount of a compound targeted to a PTP1B nucleic acid.
• provided are methods of treating an individual comprising administering one or more conjugated pharmaceutical compositions as described herein.
• the individual has metabolic related disease.
• administration of a conjugated antisense compound targeted to a PTP1B nucleic acid results in reduction of PTP1B expression by at least about 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95 or 99%, or a range defined by any two of these values.
• compositions comprising a conjugated antisense compound targeted to PTP1B are used for the preparation of a medicament for treating a patient suffering or susceptible to metabolic related disease.
• the methods described herein include administering a compound comprising a conjugate group and a modified oligonucleotide having a contiguous nucleobases portion as described herein of a sequence recited in SEQ ID NOs: 54-57. It will be understood that any of the compounds described herein can be used in the aforementioned methods and uses.
• a conjugated antisense compound targeted to a PTPIB nucleic acid in the aforementioned methods and uses can include, but is not limited to, a conjugated antisense compound targeted to SEQ ID NO: 1 comprising an at least 8 consecutive nucleobase sequence of any of SEQ ID NOs: 54-57; a conjugated antisense compound targeted to SEQ ID NO: 1 comprising a nucleobase sequence of any of SEQ ID NOs: 54-57; a compound comprising or consisting of ISIS 142082, ISIS 404173, ISIS 409826, or ISIS 446431 and a conjugate group; a compound comprising an antisense oligonucleotide disclosed in US 7,563,884 and WO 2007/131237, which is incorporated by reference in its entirety herein, and a conjugate group; a compound comprising an antisense oligonucleotide having a nucleobase sequence of any of SEQ ID NOs 17-96 and 244-3
• Obesity is considered a long-term metabolic disease. There are several serious medical sequelae related to obesity. There are over 1 billion overweight individuals worldwide with 100 million clinically obese. The increasing health care costs of treating obesity related diseases in the US alone are estimated at over $100 billion annually. Current methods for treating obesity include behavioral modification, diet, surgery
• Diabetes is a significant risk factor for both heart disease and stroke and is the leading cause of blindness and end-stage renal failure.
• Diabetes is a disorder characterized by hyperglycemia due to deficient insulin action that may result from reduced insulin production or insulin resistance or both.
• Diabetes mellitus is a polygenic disorder affecting a significant portion of the people in the world. It is divided into two types. In type I diabetes, or insulin- dependent diabetes mellitus (IDDM), patients produce little or no insulin, the hormone that regulates glucose utilization.
• IDDM insulin- dependent diabetes mellitus
• Type 2 diabetes or noninsulin-dependent diabetes mellitus (NIDDM)
• NIDDM noninsulin-dependent diabetes mellitus
• patients often have plasma insulin levels that are the same compared to nondiabetic humans; however, these patients have developed a resistance to the insulin stimulating effect of glucose and lipid metabolism in the main insulin- sensitive tissues, i.e., muscle, liver and adipose tissues, and the plasma insulin levels are insufficient to overcome the pronounced insulin resistance.
• glucotoxicity which results from long-term hyperglycemia, induces tissue-dependent insulin resistance (Nawano et al, Am. J. Physiol. Endocrinol. Metab., 278, E535-543) exacerbating the disease.
• Type 2 diabetes accounts for over 90% of all diabetes cases. It is a metabolic disorder characterized by hyperglycemia leading to secondary complications such as neuropathy, nephropathy, retinopathy, hypertriglyceridemia, obesity, and other cardiovascular diseases generally referred
• Metabolic syndrome is a combination of medical disorders that increase one's risk for
• Metabolic syndrome is known under various other names, such as (metabolic) syndrome X, insulin resistance syndrome or Reaven's syndrome.
• Diabetes and obesity are interrelated in that obesity is known to exacerbate the pathology of diabetes and greater than 60% of diabetics are obese. Most human obesity is associated with insulin resistance and leptin resistance. In fact, it has been suggested that obesity may have an even greater impact on insulin action than diabetes itself (Sindelka et al., Physiol Res., 51, 85-91). Additionally, several compounds on the market for the treatment of diabetes are known to induce weight gain, a very undesirable side effect to the treatment of this disease. Therefore, a compound that has the potential to treat both diabetes and obesity would provide a significant improvement over current treatments.
• Fibroblast growth factor receptor 4 also known as FGF receptor-4, TKF; tyrosine kinase related to fibroblast growth factor receptor; hydroxyaryl-protein kinase; tyrosylprotein kinase; Fgfr4; FGFR-4; FGFR4; CD334, FGFR4 HUMAN and JTK2
• FGF receptor-4 FGF receptor-4
• TKF tyrosine kinase related to fibroblast growth factor receptor
• hydroxyaryl-protein kinase tyrosylprotein kinase
• Fgfr4 FGFR-4
• FGFR4 FGFR4
• CD334, FGFR4 HUMAN and JTK2 FGFR4 HUMAN and JTK2
• FGFR4 Although FGFRs generally have been shown to have wide distribution throughout the bod ⁇ ', to date, FGFR4 has only been found in a few tissues. Among a wide variety of cells and tissues tested, including human lymphocytes and macrophages, FGFR4 was found to be expressed in the lung and in some tumors of lung origin as well as in malignancies not derived from lung tissues. (Holtrich et al., Proc. Nat. Acad. Sci.,. 88, 10411-10415). FGFR4 has also been found to be expressed in the liver and in adipose tissues. (Patel et al., JCEM, 90(2), 1226-1232). FGFR4 has also been found to be expressed in certain carcinoma cell lines. (Bange et al, Cancer Res., 62, 840-847).
• FGFR4 has been shown to play a role in systemic lipid and glucose homeostasis.
• FGFR4-deficient mice on a normal diet exhibited features of metabolic syndrome that include increase mass of insulin resistance, in addition to hypercholesterolemia.
• FGFR4 deficiency was shown to alleviate high-fat diet-induced fatty liver in a certain obese mouse model, which is also a correlate of metabolic syndrome.
• Antisense inhibition of FGFR4 provides a unique advantage over traditional small molecule inhibitors in that antisense inhibitors do not rely on competitive binding of the compound to the protein and inhibit activity directly by reducing the expression of FGFR4.
• a representative United States patent that teaches FGFR4 antisense inhibitors includes US. Pat. Publication No. US2010/0292140, of which is herein incorporated by reference in its entirety.
• Antisense technology is emerging as an effective means for reducing the expression of certain gene products and may therefore prove to be uniquely useful in a number of therapeutic, diagnostic, and research applications for the modulation of FGFR4.
• This invention relates to the discovery of novel, highly potent inhibitors of FGFR4 gene expression.
• conjugated antisense compounds are targeted to a FGFR4 nucleic acid having the sequence GENBANK Accession No. NM 002011.3 (incorporated herein as SEQ ID NO: 3), GENBANK Accession No: NT_023133.11 truncated from nucleosides 21323018 to 21335213 (incorporated herein as SEQ ID NO: 4); and GENBANK Accession No. AB209631.1 (incorporated herein as SEQ ID NO: 5); and GENBANK Accession No NM_022963.2 (incorporated herein as SEQ ID NO: 6).
• a conjugated antisense compound targeted to SEQ ID NOs: 3-6 is at least 90%, at least 95%, or 100% complementary to SEQ ID NOs: 3-6.
• a conjugated antisense compound targeted to SEQ ID NO: 3 comprises an at least 8 consecutive nucleobase sequence of any one of SEQ ID NOs: 58-65.
• a conjugated antisense compound targeted to SEQ ID NO: 3 comprises a nucleobase sequence of any one of SEQ ID NO: 12-19.
• a conjugated antisense compound targeted to SEQ ID NO: 3 comprises an at least 8 consecutive nucleobase sequence of SEQ ID NO: 58.
• a conjugated antisense compound targeted to SEQ ID NO: 3 comprises a nucleobase sequence of SEQ ID NO: 59.
• a conjugated antisense compound targeted to SEQ ID NO: 3 comprises an at least 8 consecutive nucleobase sequence of SEQ ID NO: 59. In certain embodiments, a conjugated antisense compound targeted to SEQ ID NO: 3 comprises a nucleobase sequence of SEQ ID NO: 59.
• a conjugated antisense compound targeted to SEQ ID NO: 3 comprises an at least 8 consecutive nucleobase sequence of SEQ ID NO: 60. In certain embodiments, a conjugated antisense compound targeted to SEQ ID NO: 3 comprises a nucleobase sequence of SEQ ID NO: 60.
• a conjugated antisense compound targeted to SEQ ID NO: 3 comprises an at least 8 consecutive nucleobase sequence of SEQ ID NO: 61. In certain embodiments, a conjugated antisense compound targeted to SEQ ID NO: 3 comprises a nucleobase sequence of SEQ ID NO: 61.
• a conjugated antisense compound targeted to SEQ ID NO: 3 comprises an at least 8 consecutive nucleobase sequence of SEQ ID NO: 62. In certain embodiments, a conjugated antisense compound targeted to SEQ ID NO: 3 comprises a nucleobase sequence of SEQ ID NO: 62. In certain embodiments, a conjugated antisense compound targeted to SEQ ID NO: 3 comprises an at least 8 consecutive nucleobase sequence of SEQ ID NO: 63. In certain embodiments, a conjugated antisense compound targeted to SEQ ID NO: 3 comprises a nucleobase sequence of SEQ ID NO: 63.
• a conjugated antisense compound targeted to SEQ ID NO: 3 comprises an at least 8 consecutive nucleobase sequence of SEQ ID NO: 64. In certain embodiments, a conjugated antisense compound targeted to SEQ ID NO: 3 comprises a nucleobase sequence of SEQ ID NO: 64.
• a conjugated antisense compound targeted to SEQ ID NO: 3 comprises an at least 8 consecutive nucleobase sequence of SEQ ID NO: 65. In certain embodiments, a conjugated antisense compound targeted to SEQ ID NO: 3 comprises a nucleobase sequence of SEQ ID NO: 65.
• a compound comprises or consists of ISIS 299005 and a conjugate group.
• ISIS 299005 is a modified oligonucleotide having the formula: Ges Ges mCes Aes mCes Ads mCds Tds mCds Ads Gds mCds Ads Gds Gds Aes mCes mCes mCes mCe, wherein
• A an adenine
• mC a 5'-methylcytosine
• G a guanine
• T a thymine
• e a 2'-0-methoxyethyl modified nucleoside
• d a 2'-deoxynucleoside
• s a phosphorothioate internucleoside linkage
• a compound comprises or consists of ISIS 463588 and a conjugate group.
• ISIS 463588 is a modified oligonucleotide having the formula: Ges mCes Aes mCes Aes mCds Tds mCds Ads Gds mCds Ads Gds Gds Ads mCes mCes mCes mCes mCe, wherein
• A an adenine
• mC a 5'-methylcytosine
• G a guanine
• T a thymine
• e a 2'-0-methoxyethyl modified nucleoside
• d a 2'-deoxynucleoside
• s a phosphorothioate intemucleoside linkage
• a compound comprises or consists of ISIS 463589 and a conjugate group.
• ISIS 463589 is a modified oligonucleotide having the formula: Aes Ges Ges mCes Aes mCds Ads mCds Tds mCds Ads Gds mCds Ads Gds Ges Aes mCes mCes mCe, wherein
• A an adenine
• mC a 5 1 -methylcytosine
• G a guanine
• T a thymine
• e a 2'-0-methoxyethyl modified nucleoside
• d a 2'-deoxynucleoside
• s a phosphorothioate intemucleoside linkage
• a compound comprises or consists of ISIS 463690 and a conjugate group.
• ISIS 463690 is a modified oligonucleotide having the formula: Ges mCes mCes Aes Ges Gds mCds Gds Ads mCds Tds Gds mCds mCds Tes mCes mCes Tes Te, wherein
• A an adenine
• mC a 5'-methylcytosine
• G a guanine
• T a thymine
• e a 2'-0-methoxyethyl modified nucleoside
• d a 2'-deoxynucleoside
• s a phosphorothioate intemucleoside linkage
• a compound comprises or consists of ISIS 463691 and a conjugate group.
• ISIS 463691 is a modified oligonucleotide having the formula: Tes Ges mCes mCes Aes Gds Gds mCds Gds Ads mCds Tds Gds mCds mCds mCes Tes mCes mCes Te, wherein
• A an adenine
• mC a 5'-methylcytosine
• G a guanine
• T a thymine
• e a 2'-0-methoxyethyl modified nucleoside
• d a 2'-deoxynucleoside
• s a phosphorothioate internucleoside linkage.
• a compound comprises or consists of ISIS 463835 and a conjugate group.
• ISIS 463835 is a modified oligonucleotide having the formula: mCes Ges mCes Tes mCes Tds mCds mCds Ads Tds mCds Ads mCds Gds Ads Ges Aes mCes Tes mCe, wherein
• A an adenine
• mC a 5'-methylcytosine
• G a guanine
• T a thymine
• e a 2'-0-methoxyethyl modified nucleoside
• d a 2'-deoxynucleoside
• s a phosphorothioate internucleoside linkage
• a compound comprises or consists of ISIS 463837 and a conjugate group.
• ISIS 463837 is a modified oligonucleotide having the formula: mCes Aes mCes Ges mCes Tds mCds Tds mCds mCds Ads Tds mCds Ads mCds Ges Aes Ges Aes mCe, wherein
• A an adenine
• mC a 5'-methylcytosine
• G a guanine
• T a thymine
• e a 2'-0-methoxyethyl modified nucleoside
• d a 2'-deoxynucleoside
• s a phosphorothioate internucleoside linkage
• a compound comprises or consists of ISIS 464225 and a conjugate group.
• ISIS 464225 is a modified oligonucleotide having the formula: mCes Tes Tes mCes mCes Ads Gds mCds Tds Tds mCds Tds mCds Tds Gds Ges Ges mCes Tes mCe, wherein
• A an adenine
• mC a 5'-methylcytosine
• G a guanine
• T a thymine
• e a 2'-0-methoxyethyl modified nucleoside
• d a 2'-deoxynucleoside
• s a phosphorothioate internucleoside linkage.
• a compound comprises an antisense oligonucleotide disclosed in WO 2009/046141, which are incoiporated by reference in their entireties herein, and a conjugate group.
• a compound comprises an antisense oligonucleotide having a nucleobase sequence of any of SEQ ID NOs 21-24, 28, 29, 36, 38, 39, 43, 48, 51, 54-56, 58-60, 64-66, 92-166 disclosed in WO 2009/046141 and a conjugate group described herein.
• a compound comprises an antisense oligonucleotide having a nucleobase sequence complementary to a preferred target segment of any of SEQ ID NOs 21-24, 28, 29, 36, 38, 39, 43, 48, 51, 54-56, 58-60, 64-66, 92-166 disclosed in WO 2009/046141 and a conjugate group described herein.
• the nucleobase sequences of all of the aforementioned referenced SEQ ID NOs are incorporated by reference herein.
• provided herein are methods of treating an individual comprising administering one or more pharmaceutical compositions as described herein.
• the individual has a metabolic disease.
• conjugated compounds targeted to FGFR4 have been shown to reduce the severity of physiological symptoms of a metabolic disease, including obesity or adiposity, metabolic syndrome, diabetes mellitus, insulin resistance, diabetic dyslipidemia, and hypertriglyceridemia.
• the conjugated compounds reduced body weight, e.g., the animals continued to experience symptoms, but the symptoms were less severe compared to untreated animals.
• the conjugated compounds reduced body fat, e.g., the animals continued to experience symptoms, but the symptoms were less severe compared to untreated animals.
• the conjugated compounds reduced adipose tissue, e.g., the animals continued to experience symptoms, but the symptoms were less severe compared to untreated animals.
• the conjugated compounds appear to reduce the symptoms of obesity; e.g., animals treated for a longer period of time experienced less severe symptoms than those administered the compounds for a shorter period of time.
• the conjugated compounds appear to reduce the symptoms of diabetes; e.g., animals treated for a longer period of time experienced less severe symptoms than those administered the compounds for a shorter period of time.
• the conjugated compounds appear to inhibit weight gain; e.g., animals treated for a longer period of time experienced less severe symptoms than those administered the compounds for a shorter period of time. In other of the experiments, however, the conjugated compounds appear to reduce glucose levels; e.g., animals treated for a longer period of time experienced less severe symptoms than those administered the compounds for a shorter period of time. In other of the experiments, however, the conjugated compounds appear to increase fatty acid oxidation; e.g., animals treated for a longer period of time experienced less severe symptoms than those administered the compounds for a shorter period of time.
• the ability of the conjugated compounds exemplified below to restore function therefore demonstrates that symptoms of the disease may be reversed by treatment with a compound as described herein.
• Obesity is characterized by numerous physical and physiological symptoms. Any symptom known to one of skill in the art to be associated with obesity can be ameliorated or otherwise modulated as set forth above in the methods described above.
• the symptom is a physical symptom selected from the group consisting of increased adipose tissue mass or weight, increased weight gain, increased fat pad weight, imbalance with caloric intake and energy expenditure, increase in body fat, increase in body mass, having a body mass index (BMI) of 30 or higher, increase in body frame, increased sweating, sleep apnea , difficulty in sleeping, inability to cope with sudden physical activity, lethargy, back and joint problems, increase in breathlessness, increase in breast region adiposity, increase in abdomen size or fat, extreme hunger, or extreme fatigue.
• BMI body mass index
• the symptom is a physiological symptom selected from the group consisting of high blood pressure, hypertension, high cholesterol levels, type 2 diabetes, stroke, cardiac insufficiency, heart disease, coronary artery obstruction, breast cancer in women, gastro-oesophageal reflux disease, hip and knee arthrosis, and reduced life expectancy.
• the physical symptom is excess body weight. In certain embodiments, the symptom is excess fat mass. In certain embodiments, the symptom is a body mass index of 30 or higher. In certain embodiments, the symptom is breathlessness. In certain embodiments, the symptom is increased sweating. In certain embodiments, the symptom is sleep apnea. In certain embodiments, the symptom is difficulty in sleeping. In certain embodiments, the symptom is inability to cope with sudden physical activity. In certain embodiments, the symptom is lethargy. In certain embodiments, the symptom is back and joint problems.
• the physiological symptom is high blood pressure. In certain embodiments, the symptom is hypertension. In certain embodiments, the symptom is high cholesterol levels. In certain embodiments, the symptom is type 2 diabetes. In certain embodiments, the symptom is stroke. In certain embodiments, the symptom is cardiac insufficiency. In certain embodiments, the symptom is heart disease. In certain embodiments, the symptom is coronary artery obstruction. In certain embodiments, the symptom is breast cancer in women. In certain embodiments, the symptom is gastro-oesophageal reflux disease. In certain embodiments, the symptom is hip and knee arthrosis. In certain embodiments, the symptom is reduced life expectancy.
• Diabetes mellitus is characterized by numerous physical and physiological symptoms. Any symptom known to one of skill in the art to be associated with Type 2 diabetes can be ameliorated or otherwise modulated as set forth above in the methods described above.
• the symptom is a physical symptom selected from the group consisting of increased glucose levels, increased weight gain, frequent urination, unusual thirst, extreme hunger, extreme fatigue, blurred vision, frequent infections, tingling or numbness at the extremities, dry and itchy skin, weight loss, slow-healing sores, and swollen gums.
• the symptom is a physiological symptom selected from the group consisting of increased insulin resistance, increased glucose levels, increased fat mass, decreased metabolic rate, decreased glucose clearance, decreased glucose tolerance, decreased insulin sensitivity, decreased hepatic insulin sensitivity, increased adipose tissue size and weight, increased body fat, and increased body weight.
• the physical symptom is increased weight gain. In certain embodiments, the symptom is frequent urination. In certain embodiments, the symptom is unusual thirst. In certain
• the symptom is extreme hunger. In certain embodiments, the symptom is extreme fatigue. In certain embodiments, the symptom is blurred vision. In certain embodiments, the symptom is frequent infections. In certain embodiments, the symptom is tingling or numbness at the extremities. In certain embodiments, the symptom is dry and itchy skin. In certain embodiments, the symptom is weight loss. In certain embodiments, the symptom is slow-healing sores. In certain embodiments, the symptom is swollen gums. In certain embodiments, the symptom is increased insulin resistance. In certain embodiments, the symptom is increased fat mass. In certain embodiments, the symptom is decreased metabolic rate. In certain embodiments, the symptom is decreased glucose clearance.
• the symptom is decreased glucose tolerance. In certain embodiments, the symptom is decreased insulin sensitivity. In certain embodiments, the symptom is decreased hepatic insulin sensitivity. In certain embodiments, the symptom is increased adipose tissue size and weight. In certain embodiments, the symptom is increased body fat. In certain embodiments, the symptom is increased body weight.
• provided are methods of treating an individual comprising administering one or more pharmaceutical compositions as described herein.
• the individual has metabolic related disease.
• administration of a conjugated antisense compound targeted to a FGFR4 nucleic acid results in reduction of FGFR4 expression by at least about 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95 or 99%, or a range defined by any two of these values.
• pharmaceutical compositions comprising a conjugated antisense compound targeted to FGFR4 are used for the preparation of a medicament for treating a patient suffering or susceptible to a metabolic disease.
• the methods described herein include administering a conjugated compound comprising a modified oligonucleotide having a contiguous nucleobases portion as described herein of a sequence recited in SEQ ID NO: 58-65.
• a conjugated antisense compound targeted to a FGFR4 nucleic acid in the aforementioned methods and uses can include, but is not limited to, a conjugated antisense compound targeted to SEQ ID NO: 3 comprising an at least 8 consecutive nucleobase sequence of any one of SEQ ID NOs: 58-65; a conjugated antisense compound targeted to SEQ ID NO: 3 comprising a nucleobase sequence of any one of SEQ ID NO: 58-65; a compound comprising or consisting of ISIS 299005, ISIS 463588, ISIS 463589, ISIS 463690, ISIS 463691, ISIS 463835, ISIS 463837, or ISIS 464225 and a conjugate group; a compound comprising an antisense oligonucleotide disclosed in WO 2009/046141, which are incorporated by reference in their entireties herein, and a
• Diabetes is a chronic metabolic disorder characterized by impaired insulin secretion and/or action.
• type 2 diabetes T2DM
• insulin resistance leads to an inability of insulin to control the activity of gluconeogenic enzymes, and many subjects also exhibit inappropriate levels of circulating glucagon (GC) in the fasting and postprandial state.
• GC circulating glucagon
• Glucagon is secreted from the a-cells of the pancreatic islets and regulates glucose homeostasis through modulation of hepatic glucose production (Quesada et al., J. Endocrinol. 2008. 199: 5-19).
• Glucagon exerts its action on target tissues via the activation of glucocorticoid receptor (GCCR).
• GCCR glucocorticoid receptor
• the glucocorticoid receptor is a 62 kDa protein that is a member of the class B G-protein coupled family of receptors (Brubaker et al., Recept. Channels. 2002. 8: 179-88).
• GCCR activation leads to signal transduction by G proteins (G s a and G q ), whereby G s a activates adenylate cyclase, which causes cAMP production, resulting in an increase in levels of protein kinase A.
• G proteins G proteins
• G proteins G proteins
• G s a activates adenylate cyclase, which causes cAMP production, resulting in an increase in levels of protein kinase A.
• GCCR signaling in the liver results in increased hepatic glucose production by induction of glycogenolysis and gluconeogenesis along with inhibition of glycogenesis
• GCCR is also expressed in extrahepatic tissues, which includes heart, intestinal smooth muscle, kidney, brain, and adipose tissue (Hansen et al., Peptides. 1995. 16: 1163-1166). Development of GCCR inhibitors have been hampered by the unfavorable side effects associated with systemic GCCR inhibition, including activation of the hypothalamic-pituitary adrenal (HP A) axis.
• HP A hypothalamic-pituitary adrenal
• Inhibition of GCCR activity in the brain can lead to an increase in circulating adrenocorticotropic hormone due to feedback regulation and a consequent increase in secretion of adrenal steroids (Philibert et al, Front. Horm. Res. 1991. 19: 1-17). This, in turn, can produce a myriad of negative chronic steroid-related side- effects.
• Other studies have demonstrated that specific inactivation of GCCR resulted in hypoglycemia upon prolonged fasting (Opherk et al., Mol. Endocronol. 2004. 18: 1346-1353).
• GCCR antisense oligonucleotides results in tissue-specific accumulation and reduced GCCR expression in liver and adipose tissue (PCT Pub. No. WO2005/071080; PCT Pub. No. WO2007/035759) without affecting GCCR mRNA levels in the CNS or adrenal glands.
• antisense inhibition of GCCR mRNA expression has be shown to improve hyperglycemia and hyperlipidemia without activating the HPA axis.
• the present invention provides compositions and methods for modulating GCCR expression. Antisense compounds for modulating expression of GCCR are disclosed in the aforementioned published patent applications. However, there remains a need for additional improved compounds.
• conjugated antisense compounds are targeted to a GCCR nucleic acid having the sequence the complement of GENBANK Accession No. NT 029289.10 truncated from nucleotides 3818000 to 3980000 (incorporated herein as SEQ ID NO: 7).
• a conjugated antisense compound targeted to SEQ ID NO: 7 is at least 90%, at least 95%, or 100% complementary to SEQ ID NO: 7.
• a conjugated antisense compound targeted to SEQ ID NO: 7 comprises an at least 8 consecutive nucleobase sequence of any one of SEQ ID NOs: 66-77.
• a conjugated antisense compound targeted to SEQ ID NO: 7 comprises a nucleobase sequence of any one of SEQ ID NO: 66-77. In certain embodiments, a conjugated antisense compound targeted to SEQ ID NO: 7 comprises an at least 8 consecutive nucleobase sequence of SEQ ID NO: 66. In certain embodiments, a conjugated antisense compound targeted to SEQ ID NO: 7 comprises a nucleobase sequence of SEQ ID NO: 66.
• a conjugated antisense compound targeted to SEQ ID NO: 7 comprises an at least 8 consecutive nucleobase sequence of SEQ ID NO: 67. In certain embodiments, a conjugated antisense compound targeted to SEQ ID NO: 7 comprises a nucleobase sequence of SEQ ID NO: 67.
• a conjugated antisense compound targeted to SEQ ID NO: 7 comprises an at least 8 consecutive nucleobase sequence of SEQ ID NO: 68. In certain embodiments, a conjugated antisense compound targeted to SEQ ID NO: 7 comprises a nucleobase sequence of SEQ ID NO: 68. In certain embodiments, a conjugated antisense compound targeted to SEQ ID NO: 7 comprises an at least 8 consecutive nucleobase sequence of SEQ ID NO: 69. In certain embodiments, a conjugated antisense compound targeted to SEQ ID NO: 7 comprises a nucleobase sequence of SEQ ID NO: 69.
• a conjugated antisense compound targeted to SEQ ID NO: 7 comprises an at least 8 consecutive nucleobase sequence of SEQ ID NO: 70. In certain embodiments, a conjugated antisense compound targeted to SEQ ID NO: 7 comprises a nucleobase sequence of SEQ ID NO: 70.
• a conjugated antisense compound targeted to SEQ ID NO: 7 comprises an at least 8 consecutive nucleobase sequence of SEQ ID NO: 71. In certain embodiments, a conjugated antisense compound targeted to SEQ ID NO: 7 comprises a nucleobase sequence of SEQ ID NO: 71.
• a conjugated antisense compound targeted to SEQ ID NO: 7 comprises an at least 8 consecutive nucleobase sequence of SEQ ID NO: 72. In certain embodiments, a conjugated antisense compound targeted to SEQ ID NO: 7 comprises a nucleobase sequence of SEQ ID NO: 72.
• a conjugated antisense compound targeted to SEQ ID NO: 7 comprises an at least 8 consecutive nucleobase sequence of SEQ ID NO: 73. In certain embodiments, a conjugated antisense compound targeted to SEQ ID NO: 7 comprises a nucleobase sequence of SEQ ID NO: 73. In certain embodiments, a conjugated antisense compound targeted to SEQ ID NO: 7 comprises an at least 8 consecutive nucleobase sequence of SEQ ID NO: 74. In certain embodiments, a conjugated antisense compound targeted to SEQ ID NO: 7 comprises a nucleobase sequence of SEQ ID NO: 74.
• a conjugated antisense compound targeted to SEQ ID NO: 7 comprises an at least 8 consecutive nucleobase sequence of SEQ ID NO: 75. In certain embodiments, a conjugated antisense compound targeted to SEQ ID NO: 7 comprises a nucleobase sequence of SEQ ID NO: 75. In certain embodiments, a coniugated antisense compound targeted to SEQ ID NO: 7 comprises an at least S consecutive nucleobase sequence of SEQ ID NO: 76. In certain embodiments, a conjugated antisense compound targeted to SEQ ID NO: 7 comprises a nucleobase sequence of SEQ ID NO: 76.
• a coniugated antisense compound targeted to SEQ ID NO: 7 comprises an at least 8 consecutive nucleobase sequence of SEQ ID NO: 77.
• a conjugated antisense compound targeted to SEQ ID NO: 7 comprises a nucleobase sequence of SEQ ID NO: 77.
• a compound comprises or consists of ISIS 420470 and a conjugate group.
• ISIS 420470 is a modified oligonucleotide having the formula: Ges Ges Tes Aes Ges Ads Ads Ads Ids Ads Ids Ads Gds Ids Ids Ges Tes Tes mCes mCe, wherein
• A :: an adenine
• mC a S'-methyleytosine
• G a guanine
• a compound comprises or consists of ISIS 420476 and a conjugate group.
• ISIS 420476 is a modified oligonucleotide having the formula: Tes Tes mCes Aes Tes Gds Ids Gds Ids mCds Ids Gds mCds Ads Ids mCes Aes Tes Ges Te, wherein
• A an adenine
• mC a 5'-methylcytosine
• G a guanine
• e a 2'-0-methoxyethyl modified nucleoside
• s a phosphorothioate internucleoside linkage.
• a compound comprises or consists of ISIS 426115 and a conjugate group.
• ISIS 426115 is a modified oligonucleotide having the formula: Gee mCes Aes Ges mCes mCds Ads Ids Gds Gds Ids Gds Ads Ids mCds Aes Ges Ges Aes Ge, wherein
• A an adenine
• mC a 5'-methylcytosine
• G a guanine
• e a 2'-0-methoxyethyl modified nucleoside
• s a phosphorothioate internucleoside linkage.
• a compound comprises or consists of ISIS 426130 and a conjugate group.
• ISIS 426130 is a modified oligonucleotide having the formula: Ges mCes Aes Tes mCes mCds Ads Gds mCds Gds Ads Gds mCds Ads mCds mCes Aes Aes Ge, wherein
• A :: an adenine
• G ::: a guanine
• T ::: a thymine
• a compound comprises or consists of ISIS 426168 and a conjugate group.
• ISIS 426168 is a modified oligonucleotide having the formula: Ges Tes mCes Tes Ges Gds Ads Ids Ids Ads mCds Ads Gds mCds Ads Tes Aes Aes Aes mCe. wherein
• A an adenine
• mC a 5'-methylcytosine
• G a guanine
• e a 2'-0-methoxyethyl modified nucleoside
• s a phosphorothioate internucleoside linkage.
• a compound comprises or consists of ISIS 426172 and a conjugate group.
• ISIS 426172 is a modified oligonucleotide having the formula: roCes mCes Tes Tes Ges Gds Ids mCds Id Gds Gds Ads Tds Ids Ads mCes Aes Ges mCes Ae, wherein
• A an adenine
• mC a 5'-methylcytosine
• G a guanine
• e a 2'-0-methoxyethyl modified nucleoside
• s a phosphorothioate internucleoside linkage.
• a compound comprises or consists of ISIS 426183 and a conjugate group.
• ISIS 426183 is a modified oligonucleotide having the formula: Aes Ges mCes mCds Ads Tds Gds Gds Tds Gds Ads Ids mCds Ads Gds Gds Ads Ges Ges roCe. wherein
• A :: an adenine
• e :::::: a 2 , -0-methoxyethyl modified nucleoside.
• a compound comprises or consists of ISIS 426246 and a conjugate group.
• ISIS 426246 is a modified oligonucleotide having the formula: Ges Ges Tes mCds Ids Gds Gds Ads Ids Ids Ads mCds Ads Gds mCds Ads Ids Aes Aes Ae, wherein
• A an adenine
• mC a 5'-methylcytosine
• G a guanine
• e a 2'-0-methoxyethyl modified nucleoside
• s a phosphorothioate intemucleoside linkage.
• a compound comprises or consists of ISIS 426261 and a conjugate group.
• 426261 is a modified oligonucleotide having the formula: Aes Ges mCds mCds Ads Ids Gds Gds Ids Gds Ads Ids mCds Ads Gds Ges Aes Ges Ges mCe. wherein
• A an adenine
• mC a 5 '-methyIcytosine
• G a guanine
• e a 2'-Q-methoxyethyl modified nucleoside
• s a phosphorothioate intemucleoside linkage.
• a compound comprises or consists of ISIS 426262 and a conjugate group.
• 426262 is a modified oligonucleotide having the formula: mCes Aes Gds mCds mCds Ads Ids Gds Gds Td Gds Ads Ids mCds Ads Ges Ges Aes Ges Ge, wherein
• s a phosphorothioate internucleoside linkage.
• a compound comprises or consists of ISIS 426267 and a conjugate group.
• ISIS 426267 is a modified oligonucleotide having the formula: Ges Tes Gds mCds Ids Ids Gds Ids mCds mCds Ads Gds Gds Ads Tds Ges Aes Tes Ges mCe, wherein
• A ⁇ an adenine.
• mC a 5'-methylcytosine
• G a guanine
• e a -O-methoxyethyl modified nucleoside
• s a phosphorothioate internucleoside linkage.
• a compound comprises or consists of ISIS 426325 and a conjugate group.
• ISIS 426325 is a modified oligonucleotide having the formula: mCes mCes Ids Ids Gds Gds Ids mCds Ids Gds Gds Ads ' Ids Ids Ads mCes Aes Ges mCes Ae, wherein
• A an adenine
• mC a 5 '-methylcytosine
• G a guanine
• e a 2'-Q-methoxvethyl modified nucleoside
• s a phosphorothioate internucleoside linkage.
• a compound comprises an antisense oligonucleotide disclosed in WO 2005/071080., WO 2007/035759, or WO 2007/136988, which are incorporated by reference in their entireties herein, and a conjugate group.
• a compound comprises an antisense oligonucleotide having a nucleobase sequence of any of SEQ ID NOs 30-216, and 306-310 disclosed in WO 2005/071080 and a conjugate group described herein.
• a compound comprises an antisense oligonucleotide having a nucleobase sequence of any of SEQ ID NOs 26-113 disclosed in WO 2007/035759 and a conjugate group described herein.
• a compound comprises an antisense oligonucleotide having a nucleobase sequence of any of SEQ ID NOs 413-485 disclosed in WO 2007/136988 and a conjugate group described herein.
• a compound comprises an antisense oligonucleotide having a nucleobase sequence complementary to a preferred target segment of any of SEQ ID NOs 30-216, and 306-310 disclosed in WO 2005/071080, 26-113 disclosed in WO 2007/035759., and 413- 485 disclosed in WO 2007/136988, and a conjugate group described herein.
• the nucleobase sequences of all of the aforementioned referenced SEQ ID N Os are incorporated by reference herein.

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