TR201806812T4 - Rna-yönlendirmeli hedef dna modifikasyonu için ve rna-yönlendirmeli transkripsiyon modifikasyonu için yöntemler ve bileşimler. - Google Patents

Rna-yönlendirmeli hedef dna modifikasyonu için ve rna-yönlendirmeli transkripsiyon modifikasyonu için yöntemler ve bileşimler. Download PDF

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Publication number
TR201806812T4
TR201806812T4 TR2018/06812T TR201806812T TR201806812T4 TR 201806812 T4 TR201806812 T4 TR 201806812T4 TR 2018/06812 T TR2018/06812 T TR 2018/06812T TR 201806812 T TR201806812 T TR 201806812T TR 201806812 T4 TR201806812 T4 TR 201806812T4
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Turkey
Prior art keywords
present
rna
target dna
methods
modification
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TR2018/06812T
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English (en)
Inventor
Charpentier Emmanuelle
Jinek Martin
Harrison Doudna Cate James
Lim Wendell
Qi Lei
Chylinski Krzysztof
A Doudna Jennifer
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Charpentier Emmanuelle
Univ California
Univ Vienna
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=49624232&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=TR201806812(T4) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Charpentier Emmanuelle, Univ California, Univ Vienna filed Critical Charpentier Emmanuelle
Publication of TR201806812T4 publication Critical patent/TR201806812T4/tr

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Abstract

Bu buluş, bir hedefleme sekansı içeren ve bir modifiye edici polipeptid ile birlikte, bir hedef DNA'da ve/veya o hedef DNA ile ilişkili bir polipeptidde yer-spesifik modifikasyon yapılmasına olanak sağlayan bir DNA-hedefleyen RNA ortaya koyar. Bu buluş, yer-spesifik modifiye edici polipeptidler de ortaya koyar. Bu buluş, bir hedef DNA'da ve/veya o hedef DNA ile ilişkili bir polipeptidde yer-spesifik modifikasyon yapmaya yarayan yöntemler de ortaya koyar. Bu buluş, bir hedef hücrede yer alan bir hedef nükleik asidin transkripsiyonunu modüle etmeyi sağlayan ve genel itibarıyla hedef nükleik asidin bir enzimatik olarak inaktif Cas9 polipeptidi ve bir DNA-hedefleyen RNA ile temas ettirilmesine dayanan yöntemler ortaya koyar. Bu yöntemleri gerçekleştirmek için kullanılabilecek kitler ve bileşimler de bu buluş kapsamında sunulmaktadır. Bu buluş, insan olmayan Cas9 transgenik çokhücreli organizmalar ve ayrıca, Cas9 üreten genetiği değiştirilmiş hücreler ortaya koyar.
TR2018/06812T 2012-05-25 2013-03-15 Rna-yönlendirmeli hedef dna modifikasyonu için ve rna-yönlendirmeli transkripsiyon modifikasyonu için yöntemler ve bileşimler. TR201806812T4 (tr)

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