NZ730667A - Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription - Google Patents
Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcriptionInfo
- Publication number
- NZ730667A NZ730667A NZ730667A NZ73066713A NZ730667A NZ 730667 A NZ730667 A NZ 730667A NZ 730667 A NZ730667 A NZ 730667A NZ 73066713 A NZ73066713 A NZ 73066713A NZ 730667 A NZ730667 A NZ 730667A
- Authority
- NZ
- New Zealand
- Prior art keywords
- rna
- present disclosure
- target dna
- methods
- directed
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 4
- 239000000203 mixture Substances 0.000 title abstract 2
- 238000013518 transcription Methods 0.000 title abstract 2
- 230000035897 transcription Effects 0.000 title abstract 2
- 230000008836 DNA modification Effects 0.000 title 1
- 229920001184 polypeptide Polymers 0.000 abstract 5
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 5
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 5
- 108020004414 DNA Proteins 0.000 abstract 4
- 108091033409 CRISPR Proteins 0.000 abstract 3
- 108091032973 (ribonucleotides)n+m Proteins 0.000 abstract 2
- 238000012986 modification Methods 0.000 abstract 2
- 230000004048 modification Effects 0.000 abstract 2
- 108020004707 nucleic acids Proteins 0.000 abstract 2
- 102000039446 nucleic acids Human genes 0.000 abstract 2
- 150000007523 nucleic acids Chemical class 0.000 abstract 2
- 230000008685 targeting Effects 0.000 abstract 1
- 230000009261 transgenic effect Effects 0.000 abstract 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
Abstract
The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms.
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261652086P | 2012-05-25 | 2012-05-25 | |
| US201261716256P | 2012-10-19 | 2012-10-19 | |
| US201361757640P | 2013-01-28 | 2013-01-28 | |
| US201361765576P | 2013-02-15 | 2013-02-15 | |
| NZ728024A NZ728024A (en) | 2012-05-25 | 2013-03-15 | Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| NZ730667A true NZ730667A (en) | 2019-05-31 |
Family
ID=66674269
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NZ730667A NZ730667A (en) | 2012-05-25 | 2013-03-15 | Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription |
| NZ753426A NZ753426A (en) | 2012-05-25 | 2013-03-15 | Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NZ753426A NZ753426A (en) | 2012-05-25 | 2013-03-15 | Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription |
Country Status (1)
| Country | Link |
|---|---|
| NZ (2) | NZ730667A (en) |
-
2013
- 2013-03-15 NZ NZ730667A patent/NZ730667A/en unknown
- 2013-03-15 NZ NZ753426A patent/NZ753426A/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| NZ753426A (en) | 2021-12-24 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PSEA | Patent sealed | ||
| RENW | Renewal (renewal fees accepted) |
Free format text: PATENT RENEWED FOR 1 YEAR UNTIL 15 MAR 2021 BY COMPUTER PACKAGES INC Effective date: 20200218 |
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| RENW | Renewal (renewal fees accepted) |
Free format text: PATENT RENEWED FOR 1 YEAR UNTIL 15 MAR 2022 BY COMPUTER PACKAGES INC Effective date: 20210306 |
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| RENW | Renewal (renewal fees accepted) |
Free format text: PATENT RENEWED FOR 1 YEAR UNTIL 15 MAR 2023 BY COMPUTER PACKAGES INC Effective date: 20220217 |
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| RENW | Renewal (renewal fees accepted) |
Free format text: PATENT RENEWED FOR 1 YEAR UNTIL 15 MAR 2024 BY COMPUTER PACKAGES INC Effective date: 20230217 |
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| RENW | Renewal (renewal fees accepted) |
Free format text: PATENT RENEWED FOR 1 YEAR UNTIL 15 MAR 2025 BY COMPUTER PACKAGES INC Effective date: 20240217 |