NZ753426A - Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription - Google Patents
Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcriptionInfo
- Publication number
- NZ753426A NZ753426A NZ753426A NZ75342613A NZ753426A NZ 753426 A NZ753426 A NZ 753426A NZ 753426 A NZ753426 A NZ 753426A NZ 75342613 A NZ75342613 A NZ 75342613A NZ 753426 A NZ753426 A NZ 753426A
- Authority
- NZ
- New Zealand
- Prior art keywords
- rna
- dna
- directed
- nucleotides
- complementary
- Prior art date
Links
- 230000008836 DNA modification Effects 0.000 title 1
- 238000000034 method Methods 0.000 title 1
- 239000000203 mixture Substances 0.000 title 1
- 238000013518 transcription Methods 0.000 title 1
- 230000035897 transcription Effects 0.000 title 1
- 108091032973 (ribonucleotides)n+m Proteins 0.000 abstract 6
- 239000002773 nucleotide Substances 0.000 abstract 6
- 125000003729 nucleotide group Chemical group 0.000 abstract 6
- 230000000295 complement effect Effects 0.000 abstract 4
- 102000040650 (ribonucleotides)n+m Human genes 0.000 abstract 3
- 108091033409 CRISPR Proteins 0.000 abstract 3
- 229920001184 polypeptide Polymers 0.000 abstract 3
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 3
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 3
- 108091079001 CRISPR RNA Proteins 0.000 abstract 2
- 108020004414 DNA Proteins 0.000 abstract 2
- 108020004707 nucleic acids Proteins 0.000 abstract 2
- 102000039446 nucleic acids Human genes 0.000 abstract 2
- 150000007523 nucleic acids Chemical class 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 2
- 108091028113 Trans-activating crRNA Proteins 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 abstract 1
- 210000003527 eukaryotic cell Anatomy 0.000 abstract 1
- 230000008685 targeting Effects 0.000 abstract 1
Landscapes
- Saccharide Compounds (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present disclosure provides a genetically modified eukaryotic cell comprising a DNA-targeting RNA and/or one or more nucleic acids that encode the DNA targeting RNA, wherein the DNA-targeting RNA comprises: (i) a DNA-targeting segment comprising a nucleotide sequence that is complementary to a sequence in the target DNA, and (ii) a protein-binding segment that interacts with a Cas9 polypeptide, wherein the protein-binding segment comprises two complementary stretches of nucleotides that hybridize to form a double stranded RNA (dsRNA) duplex, wherein said two complementary stretches of nucleotides are not linked to one another by intervening nucleotides, wherein said dsRNA duplex comprises complementary nucleotides of a tracrRNA and a CRISPR RNA (crRNA). The cell may further comprise a a Cas9 polypeptide and/or a nucleic acid comprising a nucleotide sequence that encodes the Cas9 polypeptide.
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261652086P | 2012-05-25 | 2012-05-25 | |
| US201261716256P | 2012-10-19 | 2012-10-19 | |
| US201361757640P | 2013-01-28 | 2013-01-28 | |
| US201361765576P | 2013-02-15 | 2013-02-15 | |
| NZ728024A NZ728024A (en) | 2012-05-25 | 2013-03-15 | Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| NZ753426A true NZ753426A (en) | 2021-12-24 |
Family
ID=66674269
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NZ753426A NZ753426A (en) | 2012-05-25 | 2013-03-15 | Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription |
| NZ730667A NZ730667A (en) | 2012-05-25 | 2013-03-15 | Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NZ730667A NZ730667A (en) | 2012-05-25 | 2013-03-15 | Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription |
Country Status (1)
| Country | Link |
|---|---|
| NZ (2) | NZ753426A (en) |
-
2013
- 2013-03-15 NZ NZ753426A patent/NZ753426A/en unknown
- 2013-03-15 NZ NZ730667A patent/NZ730667A/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| NZ730667A (en) | 2019-05-31 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| WO2018015936A3 (en) | Methods and compositions for modifying genomic dna | |
| PE20190843A1 (en) | RNA ADDRESSING TO GENERIC DNA | |
| EA201892810A1 (en) | HYBRID SEQUENCE OF NUCLEIC ACIDS FOR GENOMIC ENGINEERING | |
| MX2024007924A (en) | Multiplexed genome editing. | |
| MX2021009235A (en) | Improved methods for modification of target nucleic acids. | |
| GB2569733A (en) | RNA-guided nucleic acid modifying enzymes and methods of use thereof | |
| EP3699280A3 (en) | Novel cas9 systems and methods of use | |
| EA201691581A1 (en) | METHODS AND COMPOSITIONS FOR IMPROVING THE EFFICIENCY OF DIRECTED MODIFICATION OF GENES WITH THE APPLICATION OF MEDIATED OLIGONUCLEOTIDE REPAIR GENES | |
| WO2016183438A8 (en) | Self-targeting genome editing system | |
| CA2884084C (en) | Fad2 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks | |
| CA3010891A1 (en) | Recombinogenic nucleic acid strands in situ | |
| WO2011101811A3 (en) | Improved meganuclease recombination system | |
| NZ601247A (en) | Targeted genomic alteration | |
| MX2018010529A (en) | Compositions and methods for the improved production and delivery of rna by efficient transcription termination. | |
| WO2014186585A3 (en) | Methods and compositions for treatment of a genetic condition | |
| WO2014022702A3 (en) | Methods and compositions for controlling gene expression by rna processing | |
| EP4397759A3 (en) | Composition for cleaving a target dna comprising a guide rna specific for the target dna and cas protein-encoding nucleic acid or cas protein, and use thereof | |
| EA201291024A1 (en) | COMPOSITIONS OF ENDORIBONUCLEAS AND METHODS OF THEIR USE | |
| WO2014172470A3 (en) | Methods of mutating, modifying or modulating nucleic acid in a cell or nonhuman mammal | |
| EP4282970A3 (en) | Rna-guided human genome engineering | |
| MX2015017110A (en) | Targeted integration. | |
| NZ705745A (en) | Fad3 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks | |
| GEP201606544B (en) | Excision of transgenes in genetically modified organisms | |
| EA201891629A1 (en) | METHODS AND COMPOSITIONS FOR IMPROVING THE EFFICIENCY OF TARGETED MODIFICATION OF GENES WITH THE APPLICATION OF MEDIATED OLIGONUCLEOTIDE REPAIR GENES | |
| IN2015MN00436A (en) |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PSEA | Patent sealed | ||
| RENW | Renewal (renewal fees accepted) |
Free format text: PATENT RENEWED FOR 1 YEAR UNTIL 15 MAR 2024 BY COMPUTER PACKAGES INC Effective date: 20230217 |
|
| RENW | Renewal (renewal fees accepted) |
Free format text: PATENT RENEWED FOR 1 YEAR UNTIL 15 MAR 2025 BY COMPUTER PACKAGES INC Effective date: 20240217 |