NZ753426A - Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription - Google Patents

Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription

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Publication number
NZ753426A
NZ753426A NZ753426A NZ75342613A NZ753426A NZ 753426 A NZ753426 A NZ 753426A NZ 753426 A NZ753426 A NZ 753426A NZ 75342613 A NZ75342613 A NZ 75342613A NZ 753426 A NZ753426 A NZ 753426A
Authority
NZ
New Zealand
Prior art keywords
rna
dna
directed
nucleotides
complementary
Prior art date
Application number
NZ753426A
Inventor
Emmanuelle Charpentier
Jennifer A Doudna
Martin Jinek
Krzysztof Chylinski
Cate James Harrison Doudna
Wendell Lim
Lei Qi
Original Assignee
Univ California
Univ Vienna
Emmanuelle Charpentier
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ California, Univ Vienna, Emmanuelle Charpentier filed Critical Univ California
Priority claimed from NZ728024A external-priority patent/NZ728024A/en
Publication of NZ753426A publication Critical patent/NZ753426A/en

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Abstract

The present disclosure provides a genetically modified eukaryotic cell comprising a DNA-targeting RNA and/or one or more nucleic acids that encode the DNA targeting RNA, wherein the DNA-targeting RNA comprises: (i) a DNA-targeting segment comprising a nucleotide sequence that is complementary to a sequence in the target DNA, and (ii) a protein-binding segment that interacts with a Cas9 polypeptide, wherein the protein-binding segment comprises two complementary stretches of nucleotides that hybridize to form a double stranded RNA (dsRNA) duplex, wherein said two complementary stretches of nucleotides are not linked to one another by intervening nucleotides, wherein said dsRNA duplex comprises complementary nucleotides of a tracrRNA and a CRISPR RNA (crRNA). The cell may further comprise a a Cas9 polypeptide and/or a nucleic acid comprising a nucleotide sequence that encodes the Cas9 polypeptide.
NZ753426A 2012-05-25 2013-03-15 Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription NZ753426A (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261652086P 2012-05-25 2012-05-25
US201261716256P 2012-10-19 2012-10-19
US201361757640P 2013-01-28 2013-01-28
US201361765576P 2013-02-15 2013-02-15
NZ728024A NZ728024A (en) 2012-05-25 2013-03-15 Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription

Publications (1)

Publication Number Publication Date
NZ753426A true NZ753426A (en) 2021-12-24

Family

ID=66674269

Family Applications (2)

Application Number Title Priority Date Filing Date
NZ753426A NZ753426A (en) 2012-05-25 2013-03-15 Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription
NZ730667A NZ730667A (en) 2012-05-25 2013-03-15 Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription

Family Applications After (1)

Application Number Title Priority Date Filing Date
NZ730667A NZ730667A (en) 2012-05-25 2013-03-15 Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription

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NZ (2) NZ753426A (en)

Also Published As

Publication number Publication date
NZ730667A (en) 2019-05-31

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