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Priority claimed from NZ716606Aexternal-prioritypatent/NZ716606B2/en
Publication of NZ754901ApublicationCriticalpatent/NZ754901A/en
Micro-Organisms Or Cultivation Processes Thereof
(AREA)
Abstract
The present invention relates to improved methods of genome engineering in cells using a TALEN lacking repeat sequences or Cas9. More specifically, the present invention provides an isolated stem cell including a first foreign nucleic acid integrated into the genome of the cell and encoding for a Cas9 enzyme that forms a co-localization complex with guide RNA complementary to target DNA and that cleaves the target DNA in a site specific manner, with the proviso that the isolated stem cell excludes a human totipotent stem cell.
Composition for cleaving a target dna comprising a guide rna specific for the target dna and cas protein-encoding nucleic acid or cas protein, and use thereof
Telomerase and Retrotransposons: Reverse Transcriptases That Shaped Genomes Special Feature Sackler Colloquium: Similarities between long interspersed element-1 (LINE-1) reverse transcriptase and telomerase