EP4349980A3 - Methods and compositions for the targeted modification of a genome - Google Patents
Methods and compositions for the targeted modification of a genome Download PDFInfo
- Publication number
- EP4349980A3 EP4349980A3 EP24157885.5A EP24157885A EP4349980A3 EP 4349980 A3 EP4349980 A3 EP 4349980A3 EP 24157885 A EP24157885 A EP 24157885A EP 4349980 A3 EP4349980 A3 EP 4349980A3
- Authority
- EP
- European Patent Office
- Prior art keywords
- methods
- compositions
- genome
- human
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title abstract 4
- 239000000203 mixture Substances 0.000 title abstract 3
- 238000012986 modification Methods 0.000 title 1
- 230000004048 modification Effects 0.000 title 1
- 210000004962 mammalian cell Anatomy 0.000 abstract 2
- 238000010453 CRISPR/Cas method Methods 0.000 abstract 1
- 210000003527 eukaryotic cell Anatomy 0.000 abstract 1
- 238000012239 gene modification Methods 0.000 abstract 1
- 230000005017 genetic modification Effects 0.000 abstract 1
- 235000013617 genetically modified food Nutrition 0.000 abstract 1
- 210000004602 germ cell Anatomy 0.000 abstract 1
- 210000005260 human cell Anatomy 0.000 abstract 1
- 150000007523 nucleic acids Chemical group 0.000 abstract 1
- 230000008685 targeting Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
- A01K67/0275—Genetically modified vertebrates, e.g. transgenic
- A01K67/0276—Knock-out vertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
- A01K67/0275—Genetically modified vertebrates, e.g. transgenic
- A01K67/0278—Knock-in vertebrates, e.g. humanised vertebrates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1024—In vivo mutagenesis using high mutation rate "mutator" host strains by inserting genetic material, e.g. encoding an error prone polymerase, disrupting a gene for mismatch repair
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/07—Animals genetically altered by homologous recombination
- A01K2217/072—Animals genetically altered by homologous recombination maintaining or altering function, i.e. knock in
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/07—Animals genetically altered by homologous recombination
- A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/10—Mammal
- A01K2227/105—Murine
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2267/00—Animals characterised by purpose
- A01K2267/03—Animal model, e.g. for test or diseases
- A01K2267/035—Animal model for multifactorial diseases
- A01K2267/0362—Animal model for lipid/glucose metabolism, e.g. obesity, type-2 diabetes
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2267/00—Animals characterised by purpose
- A01K2267/03—Animal model, e.g. for test or diseases
- A01K2267/035—Animal model for multifactorial diseases
- A01K2267/0387—Animal model for diseases of the immune system
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/40—Systems of functionally co-operating vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2810/00—Vectors comprising a targeting moiety
- C12N2810/10—Vectors comprising a non-peptidic targeting moiety
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2999/00—Further aspects of viruses or vectors not covered by groups C12N2710/00 - C12N2796/00 or C12N2800/00
- C12N2999/007—Technological advancements, e.g. new system for producing known virus, cre-lox system for production of transgenic animals
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Veterinary Medicine (AREA)
- Environmental Sciences (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Mycology (AREA)
- Animal Behavior & Ethology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Applications Claiming Priority (8)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201361914768P | 2013-12-11 | 2013-12-11 | |
US201462017416P | 2014-06-26 | 2014-06-26 | |
US201462029261P | 2014-07-25 | 2014-07-25 | |
US201462052906P | 2014-09-19 | 2014-09-19 | |
US201462059527P | 2014-10-03 | 2014-10-03 | |
EP14790457.7A EP3080279B1 (en) | 2013-12-11 | 2014-10-15 | Methods and compositions for the targeted modification of a genome |
PCT/US2014/060788 WO2015088643A1 (en) | 2013-12-11 | 2014-10-15 | Methods and compositions for the targeted modification of a genome |
EP18188401.6A EP3460063B1 (en) | 2013-12-11 | 2014-10-15 | Methods and compositions for the targeted modification of a genome |
Related Parent Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP18188401.6A Division EP3460063B1 (en) | 2013-12-11 | 2014-10-15 | Methods and compositions for the targeted modification of a genome |
EP14790457.7A Division EP3080279B1 (en) | 2013-12-11 | 2014-10-15 | Methods and compositions for the targeted modification of a genome |
Publications (2)
Publication Number | Publication Date |
---|---|
EP4349980A2 EP4349980A2 (en) | 2024-04-10 |
EP4349980A3 true EP4349980A3 (en) | 2024-06-26 |
Family
ID=56885593
Family Applications (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP18188401.6A Active EP3460063B1 (en) | 2013-12-11 | 2014-10-15 | Methods and compositions for the targeted modification of a genome |
EP24157885.5A Pending EP4349980A3 (en) | 2013-12-11 | 2014-10-15 | Methods and compositions for the targeted modification of a genome |
EP14790457.7A Revoked EP3080279B1 (en) | 2013-12-11 | 2014-10-15 | Methods and compositions for the targeted modification of a genome |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP18188401.6A Active EP3460063B1 (en) | 2013-12-11 | 2014-10-15 | Methods and compositions for the targeted modification of a genome |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP14790457.7A Revoked EP3080279B1 (en) | 2013-12-11 | 2014-10-15 | Methods and compositions for the targeted modification of a genome |
Country Status (11)
Country | Link |
---|---|
EP (3) | EP3460063B1 (en) |
CY (1) | CY1121367T1 (en) |
DK (1) | DK3080279T3 (en) |
ES (1) | ES2700596T3 (en) |
HR (1) | HRP20181632T1 (en) |
HU (1) | HUE041331T2 (en) |
LT (1) | LT3080279T (en) |
PL (1) | PL3080279T3 (en) |
PT (1) | PT3080279T (en) |
RS (1) | RS58159B1 (en) |
SI (1) | SI3080279T1 (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU2018274769B2 (en) * | 2017-05-25 | 2024-05-02 | Eggxyt Ltd | Methods for gender determination of avian embryos in unhatched eggs and means thereof |
CN112522206A (en) * | 2020-12-15 | 2021-03-19 | 苏州恒康生命科学有限公司 | Construction method and application of ROR1 gene knockout tumor cell strain |
Citations (3)
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WO2006044962A1 (en) * | 2004-10-19 | 2006-04-27 | Regeneron Pharmaceuticals, Inc. | Method for generating an animal homozygous for a genetic modification |
WO2007117410A2 (en) * | 2006-03-31 | 2007-10-18 | Medarex, Inc. | Transgenic animals expressing chimeric antibodies for use in preparing human antibodies |
WO2014172489A2 (en) * | 2013-04-16 | 2014-10-23 | Regeneron Pharmaceuticals, Inc. | Targeted modification of rat genome |
Family Cites Families (44)
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US3605750A (en) | 1969-04-07 | 1971-09-20 | David S Sheridan | X-ray tip catheter |
AU8587598A (en) | 1997-07-26 | 1999-02-16 | Wisconsin Alumni Research Foundation | Trans-species nuclear transfer |
US6599692B1 (en) | 1999-09-14 | 2003-07-29 | Sangamo Bioscience, Inc. | Functional genomics using zinc finger proteins |
US20030104526A1 (en) | 1999-03-24 | 2003-06-05 | Qiang Liu | Position dependent recognition of GNN nucleotide triplets by zinc fingers |
US7105348B2 (en) | 2000-10-31 | 2006-09-12 | Regeneron Pharmaceuticals, Inc. | Methods of modifying eukaryotic cells |
US6596541B2 (en) | 2000-10-31 | 2003-07-22 | Regeneron Pharmaceuticals, Inc. | Methods of modifying eukaryotic cells |
US20050144655A1 (en) | 2000-10-31 | 2005-06-30 | Economides Aris N. | Methods of modifying eukaryotic cells |
US6586251B2 (en) | 2000-10-31 | 2003-07-01 | Regeneron Pharmaceuticals, Inc. | Methods of modifying eukaryotic cells |
AU2884102A (en) | 2000-12-07 | 2002-06-18 | Sangamo Biosciences Inc | Regulation of angiogenesis with zinc finger proteins |
AU2002225187A1 (en) | 2001-01-22 | 2002-07-30 | Sangamo Biosciences, Inc. | Zinc finger polypeptides and their use |
CA2435394C (en) | 2001-01-22 | 2018-01-09 | Sangamo Biosciences, Inc. | Modified zinc finger binding proteins |
AUPR451401A0 (en) | 2001-04-20 | 2001-05-24 | Monash University | A method of nuclear transfer |
AU2003251286B2 (en) | 2002-01-23 | 2007-08-16 | The University Of Utah Research Foundation | Targeted chromosomal mutagenesis using zinc finger nucleases |
US20060078552A1 (en) | 2002-03-15 | 2006-04-13 | Sylvain Arnould | Hybrid and single chain meganucleases and use thereof |
US20030232410A1 (en) | 2002-03-21 | 2003-12-18 | Monika Liljedahl | Methods and compositions for using zinc finger endonucleases to enhance homologous recombination |
US7612250B2 (en) | 2002-07-29 | 2009-11-03 | Trustees Of Tufts College | Nuclear transfer embryo formation method |
CA2497913C (en) | 2002-09-05 | 2014-06-03 | California Institute Of Technology | Use of chimeric nucleases to stimulate gene targeting |
AU2003290518A1 (en) | 2002-09-06 | 2004-04-23 | Fred Hutchinson Cancer Research Center | Methods and compositions concerning designed highly-specific nucleic acid binding proteins |
WO2005001087A2 (en) | 2003-06-11 | 2005-01-06 | Regeneron Pharmaceuticals, Inc. | Methods of modifying genes in eukaryotic cells |
US8409861B2 (en) | 2003-08-08 | 2013-04-02 | Sangamo Biosciences, Inc. | Targeted deletion of cellular DNA sequences |
US7888121B2 (en) | 2003-08-08 | 2011-02-15 | Sangamo Biosciences, Inc. | Methods and compositions for targeted cleavage and recombination |
EP1591521A1 (en) | 2004-04-30 | 2005-11-02 | Cellectis | I-Dmo I derivatives with enhanced activity at 37 degrees C and use thereof |
CA2579677A1 (en) | 2004-09-16 | 2006-03-30 | Sangamo Biosciences, Inc. | Compositions and methods for protein production |
AU2006224248B2 (en) | 2005-03-15 | 2011-01-06 | Cellectis | I-Crei meganuclease variants with modified specificity, method of preparation and uses thereof |
WO2006097784A1 (en) | 2005-03-15 | 2006-09-21 | Cellectis | I-crei meganuclease variants with modified specificity, method of preparation and uses thereof |
CN101117633B (en) | 2006-08-03 | 2011-07-20 | 上海交通大学附属儿童医院 | Nucleus transplantation method |
JP5632610B2 (en) | 2006-12-14 | 2014-11-26 | ダウ アグロサイエンシィズ エルエルシー | Optimized non-canonical zinc finger protein |
US20110239315A1 (en) | 2009-01-12 | 2011-09-29 | Ulla Bonas | Modular dna-binding domains and methods of use |
EP2206723A1 (en) | 2009-01-12 | 2010-07-14 | Bonas, Ulla | Modular DNA-binding domains |
JP5932632B2 (en) | 2009-03-20 | 2016-06-15 | サンガモ バイオサイエンシーズ, インコーポレイテッド | Modification of CXCR4 using modified zinc finger protein |
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US9637739B2 (en) | 2012-03-20 | 2017-05-02 | Vilnius University | RNA-directed DNA cleavage by the Cas9-crRNA complex |
PT2800811T (en) | 2012-05-25 | 2017-08-17 | Univ California | Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription |
CN110643600A (en) | 2012-10-23 | 2020-01-03 | 基因工具股份有限公司 | System for cutting target DNA and use thereof |
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PL3152312T3 (en) | 2014-06-06 | 2020-08-10 | Regeneron Pharmaceuticals, Inc. | Methods and compositions for modifying a targeted locus |
-
2014
- 2014-10-15 EP EP18188401.6A patent/EP3460063B1/en active Active
- 2014-10-15 EP EP24157885.5A patent/EP4349980A3/en active Pending
- 2014-10-15 HU HUE14790457A patent/HUE041331T2/en unknown
- 2014-10-15 SI SI201430912T patent/SI3080279T1/en unknown
- 2014-10-15 ES ES14790457T patent/ES2700596T3/en active Active
- 2014-10-15 DK DK14790457.7T patent/DK3080279T3/en active
- 2014-10-15 RS RS20181418A patent/RS58159B1/en unknown
- 2014-10-15 PL PL14790457T patent/PL3080279T3/en unknown
- 2014-10-15 EP EP14790457.7A patent/EP3080279B1/en not_active Revoked
- 2014-10-15 PT PT14790457T patent/PT3080279T/en unknown
- 2014-10-15 LT LTEP14790457.7T patent/LT3080279T/en unknown
-
2018
- 2018-10-10 HR HRP20181632TT patent/HRP20181632T1/en unknown
- 2018-12-05 CY CY20181101296T patent/CY1121367T1/en unknown
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2006044962A1 (en) * | 2004-10-19 | 2006-04-27 | Regeneron Pharmaceuticals, Inc. | Method for generating an animal homozygous for a genetic modification |
WO2007117410A2 (en) * | 2006-03-31 | 2007-10-18 | Medarex, Inc. | Transgenic animals expressing chimeric antibodies for use in preparing human antibodies |
WO2014172489A2 (en) * | 2013-04-16 | 2014-10-23 | Regeneron Pharmaceuticals, Inc. | Targeted modification of rat genome |
Non-Patent Citations (6)
Title |
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J. A. DOUDNA ET AL: "The new frontier of genome engineering with CRISPR-Cas9", SCIENCE, vol. 346, no. 6213, 28 November 2014 (2014-11-28), pages 1258096 - 1258096, XP055162699, ISSN: 0036-8075, DOI: 10.1126/science.1258096 * |
L. CONG ET AL: "Multiplex Genome Engineering Using CRISPR/Cas Systems", SCIENCE, vol. 339, no. 6121, 15 February 2013 (2013-02-15), pages 819 - 823, XP055067741, ISSN: 0036-8075, DOI: 10.1126/science.1231143 * |
L. CONG ET AL: "Supplementary Material to : Multiplex Genome Engineering Using CRISPR/Cas Systems", SCIENCE, vol. 339, no. 6121, 3 January 2013 (2013-01-03), pages 819 - 823, XP055067744, ISSN: 0036-8075, DOI: 10.1126/science.1231143 * |
P. MALI ET AL: "RNA-Guided Human Genome Engineering via Cas9", SCIENCE, vol. 339, no. 6121, 15 February 2013 (2013-02-15), pages 823 - 826, XP055159412, ISSN: 0036-8075, DOI: 10.1126/science.1232033 * |
TAO XU ET AL: "Cas9-Based Tools for Targeted Genome Editing and Transcriptional Control", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 80, no. 5, 3 January 2014 (2014-01-03), US, pages 1544 - 1552, XP055540934, ISSN: 0099-2240, DOI: 10.1128/AEM.03786-13 * |
WOONG Y HWANG ET AL: "Efficient genome editing in zebrafish using a CRISPR-Cas system", NATURE BIOTECHNOLOGY, vol. 31, no. 3, 1 March 2013 (2013-03-01), pages 227 - 229, XP055540933, ISSN: 1087-0156, DOI: 10.1038/nbt.2501 * |
Also Published As
Publication number | Publication date |
---|---|
SI3080279T1 (en) | 2019-01-31 |
EP3080279B1 (en) | 2018-09-26 |
HUE041331T2 (en) | 2019-05-28 |
RS58159B1 (en) | 2019-03-29 |
EP3080279A1 (en) | 2016-10-19 |
ES2700596T3 (en) | 2019-02-18 |
EP4349980A2 (en) | 2024-04-10 |
PT3080279T (en) | 2018-12-17 |
EP3460063A1 (en) | 2019-03-27 |
DK3080279T3 (en) | 2018-11-05 |
CY1121367T1 (en) | 2020-05-29 |
EP3460063B1 (en) | 2024-03-13 |
LT3080279T (en) | 2018-10-25 |
PL3080279T3 (en) | 2019-03-29 |
HRP20181632T1 (en) | 2019-01-11 |
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