JP2015006201A5 - - Google Patents
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- JP2015006201A5 JP2015006201A5 JP2014203622A JP2014203622A JP2015006201A5 JP 2015006201 A5 JP2015006201 A5 JP 2015006201A5 JP 2014203622 A JP2014203622 A JP 2014203622A JP 2014203622 A JP2014203622 A JP 2014203622A JP 2015006201 A5 JP2015006201 A5 JP 2015006201A5
- Authority
- JP
- Japan
- Prior art keywords
- receptor
- incubator
- amplification
- temperature
- amplification incubator
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 230000003321 amplification Effects 0.000 claims 17
- 238000003199 nucleic acid amplification method Methods 0.000 claims 17
- 150000007523 nucleic acids Chemical class 0.000 claims 5
- 108020004707 nucleic acids Proteins 0.000 claims 5
- 238000000034 method Methods 0.000 claims 4
- 239000003153 chemical reaction reagent Substances 0.000 claims 2
- 238000010438 heat treatment Methods 0.000 claims 2
- 230000000087 stabilizing Effects 0.000 claims 2
- 238000001816 cooling Methods 0.000 claims 1
- 238000011105 stabilization Methods 0.000 claims 1
Claims (9)
- 所定の温度範囲内にあるインキュベーション温度において核酸増幅手順を実行するためのコンピュータ制御方法であって、前記方法は、器具内で実行され、前記器具は、前記器具の第１の位置に据えられた少なくとも１つの熱要素と、前記器具の第２の離間した位置に据えられた増幅インキュベータとを含み、
前記方法は、以下の自動化されたステップ：
（ａ）前記器具の前記第１の位置において、受容器およびその内容物を所定の時間の間、前記熱要素に曝露することであって、その結果、前記受容器の前記内容物の温度は、前記インキュベーション温度に近づくように調節される、こと；
（ｂ）前記受容器を前記第１の位置から前記増幅インキュベータの中に移動させることであって、前記増幅インキュベータは、前記受容器がその中に移動させられるときに、前記インキュベーション温度にあり、前記受容器は、前記増幅インキュベータのハウジング内の受容器アクセス開口部を通して前記増幅インキュベータの中に移動させられ、前記増幅インキュベータは、閉鎖位置と開放位置との間で移動可能なドアを含み、前記閉鎖位置は、前記受容器アクセス開口部を通した前記増幅インキュベータへのアクセスを防止し、前記開放位置は、前記受容器アクセス開口部を通した前記増幅インキュベータへのアクセスを可能にする、こと；および
（ｃ）前記受容器が前記増幅インキュベータ内にある間に、前記受容器の前記内容物に含まれる核酸に、前記核酸増幅手順を受けさせること
を含む、方法。 - 前記熱要素は、熱電性の加熱または冷却により、前記受容器の前記内容物の前記温度が、ステップ（ａ）において調節されるようにする、請求項１に記載の方法。
- 前記熱要素は、抵抗性の加熱により、前記受容器の前記内容物の前記温度が、ステップ（ａ）において調節されるようにする、請求項１に記載の方法。
- 前記第１の位置および前記第２の位置は、前記器具の処理デッキ上の異なる場所である、請求項１に記載の方法。
- 前記受容器は、複数の一体的に形成された受容器のうちの１つである、請求項１に記載の方法。
- 前記核酸増幅手順を実行するための試薬を前記受容器の中に分配する自動化されたステップをさらに含む、請求項１に記載の方法。
- 前記受容器が前記増幅インキュベータ内に配置されている間に、前記試薬が、前記受容器の中に分配される、請求項６に記載の方法。
- 前記増幅インキュベータ内の前記温度は、前記核酸増幅手順の間に、約０．１℃よりも大きく変わらない、請求項１に記載の方法。
- 前記受容器を前記第１の位置から前記増幅インキュベータの中に移動させる前に、前記受容器を安定化増幅インキュベータの中に移動させ、そして、前記受容器を所定の時間の間、前記安定化インキュベータ内で保持し、その間に、前記安定化インキュベータを安定化温度に維持する自動化されたステップ
をさらに含む、請求項１に記載の方法。
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US8392798P | 1998-05-01 | 1998-05-01 | |
| US60/083,927 | 1998-05-01 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011006319A Division JP2011078433A (ja) | 1998-05-01 | 2011-01-14 | 自動化診断用分析器および方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015006201A JP2015006201A (ja) | 2015-01-15 |
| JP2015006201A5 true JP2015006201A5 (ja) | 2015-09-17 |
| JP5936219B2 JP5936219B2 (ja) | 2016-06-22 |
Family
ID=22181564
Family Applications (12)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2000547476A Expired - Lifetime JP4511034B2 (ja) | 1998-05-01 | 1999-04-30 | 自動化診断用分析器および方法 |
| JP2007151041A Expired - Fee Related JP4662580B2 (ja) | 1998-05-01 | 2007-06-06 | 自動化診断用分析器および方法 |
| JP2008169683A Expired - Fee Related JP4550131B2 (ja) | 1998-05-01 | 2008-06-27 | 自動化診断用分析器および方法 |
| JP2008169681A Expired - Lifetime JP4518570B2 (ja) | 1998-05-01 | 2008-06-27 | 自動化診断用分析器および方法 |
| JP2008169682A Expired - Fee Related JP4550130B2 (ja) | 1998-05-01 | 2008-06-27 | 自動化診断用分析器および方法 |
| JP2008169680A Expired - Lifetime JP4526131B2 (ja) | 1998-05-01 | 2008-06-27 | 自動化診断用分析器および方法 |
| JP2008169684A Expired - Fee Related JP4942049B2 (ja) | 1998-05-01 | 2008-06-27 | 自動化診断用分析器および方法 |
| JP2011006319A Withdrawn JP2011078433A (ja) | 1998-05-01 | 2011-01-14 | 自動化診断用分析器および方法 |
| JP2011277356A Withdrawn JP2012055323A (ja) | 1998-05-01 | 2011-12-19 | 自動化診断用分析器および方法 |
| JP2013215244A Pending JP2014014374A (ja) | 1998-05-01 | 2013-10-16 | 自動化診断用分析器および方法 |
| JP2013215245A Pending JP2014006267A (ja) | 1998-05-01 | 2013-10-16 | 自動化診断用分析器および方法 |
| JP2014203622A Expired - Lifetime JP5936219B2 (ja) | 1998-05-01 | 2014-10-02 | 自動化診断用分析器および方法 |
Family Applications Before (11)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2000547476A Expired - Lifetime JP4511034B2 (ja) | 1998-05-01 | 1999-04-30 | 自動化診断用分析器および方法 |
| JP2007151041A Expired - Fee Related JP4662580B2 (ja) | 1998-05-01 | 2007-06-06 | 自動化診断用分析器および方法 |
| JP2008169683A Expired - Fee Related JP4550131B2 (ja) | 1998-05-01 | 2008-06-27 | 自動化診断用分析器および方法 |
| JP2008169681A Expired - Lifetime JP4518570B2 (ja) | 1998-05-01 | 2008-06-27 | 自動化診断用分析器および方法 |
| JP2008169682A Expired - Fee Related JP4550130B2 (ja) | 1998-05-01 | 2008-06-27 | 自動化診断用分析器および方法 |
| JP2008169680A Expired - Lifetime JP4526131B2 (ja) | 1998-05-01 | 2008-06-27 | 自動化診断用分析器および方法 |
| JP2008169684A Expired - Fee Related JP4942049B2 (ja) | 1998-05-01 | 2008-06-27 | 自動化診断用分析器および方法 |
| JP2011006319A Withdrawn JP2011078433A (ja) | 1998-05-01 | 2011-01-14 | 自動化診断用分析器および方法 |
| JP2011277356A Withdrawn JP2012055323A (ja) | 1998-05-01 | 2011-12-19 | 自動化診断用分析器および方法 |
| JP2013215244A Pending JP2014014374A (ja) | 1998-05-01 | 2013-10-16 | 自動化診断用分析器および方法 |
| JP2013215245A Pending JP2014006267A (ja) | 1998-05-01 | 2013-10-16 | 自動化診断用分析器および方法 |
Country Status (11)
| Country | Link |
|---|---|
| US (31) | US6335166B1 (ja) |
| EP (16) | EP1930078A1 (ja) |
| JP (12) | JP4511034B2 (ja) |
| KR (1) | KR100704324B1 (ja) |
| AT (7) | ATE423622T1 (ja) |
| AU (2) | AU765623B2 (ja) |
| CA (7) | CA2737980C (ja) |
| DE (14) | DE69928390T2 (ja) |
| DK (4) | DK1623764T3 (ja) |
| ES (5) | ES2322859T3 (ja) |
| WO (1) | WO1999057561A2 (ja) |
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2000
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