JP2020536542A - 安定化されたステビオール配糖体組成物及びその使用 - Google Patents
安定化されたステビオール配糖体組成物及びその使用 Download PDFInfo
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- JP2020536542A JP2020536542A JP2020519669A JP2020519669A JP2020536542A JP 2020536542 A JP2020536542 A JP 2020536542A JP 2020519669 A JP2020519669 A JP 2020519669A JP 2020519669 A JP2020519669 A JP 2020519669A JP 2020536542 A JP2020536542 A JP 2020536542A
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- JP
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- Prior art keywords
- acid
- steviol glycoside
- composition
- quinic
- ester
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
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Abstract
Description
本出願は、2017年10月6日に出願され、名称が「Steviol Glycoside Solubility Enhancers」の米国特許仮出願第62/569,279号、及び2018年5月25日に出願され、名称が「Methods for Making Yerba Mate Extract Composition」の米国特許仮出願第62/676,722号の利益を主張するものであり、これら両出願は、その全体が完全に本明細書中に記載されているかのように参照により本明細書に組み込まれる。
(a)イェルバマテバイオマスを水性組成物と接触させて、初期の抽出物を得ること;
(b)初期の抽出物から固形物を除去して、第2の初期の抽出物を得ること;
(c)水性組成物で第2の初期の抽出物の体積を調節して、調節された第2の初期の抽出物を得ること;
(d)調節された第2の初期の抽出物をイオン交換クロマトグラフィー固定相においてクロマトグラフィーにより分離すること;
(e)イオン交換クロマトグラフィー固定相を溶出して、溶媒を含む第1の溶離液を得ること;
(f)溶媒を除去して、濃縮物を形成すること;及び
(g)濃縮物の脱色及び脱塩のうちの少なくとも1つを行って、ろ液及び保持液のうちの少なくとも1つを得ること。
(a)イェルバマテバイオマスを水性組成物と接触させて、初期の抽出物を得ること;
(b)初期の抽出物から固形物を除去して、第2の初期の抽出物を得ること;
(c)水性組成物で第2の初期の抽出物の体積を調節して、調節された第2の初期の抽出物を得ること;
(d)調節された初期の抽出物をイオン交換クロマトグラフィー固定相においてクロマトグラフィーにより分離すること;
(e)イオン交換固定相を溶出して、溶媒を含む第1の溶離液を得ること;
(f)溶媒を除去して、濃縮物を形成すること;
(g)濃縮物の脱色及び脱塩のうちの少なくとも1つを行って、ろ液及び保持液のうちの少なくとも1つを得ること;及び
(h)ろ液及び保持液のうちの少なくとも1つを乾燥して、1つ以上のステビオール配糖体安定化化合物、及びその塩を含む組成物を得ること。
(i)イェルバマテバイオマスを水性組成物と接触させて、初期の抽出物を得ること;
(ii)初期の抽出物から固形物を除去して、第2の初期の抽出物を得ること;
(iii)第2の初期の抽出物を酸性化酢酸エチルと接触させて、酸性の酢酸エチル抽出物を得ること;
(iv)酸性の酢酸エチル抽出物を中和して、中和された酢酸エチル及び水性抽出物を得ること;
(v)水性抽出物を脱色して、脱色された水性抽出物を得ること;及び
(vi)脱色された水性抽出物を乾燥して、1つ以上のステビオール配糖体安定化化合物、及びその塩を含む組成物を得ること。
イェルバマテバイオマスを水性組成物と接触させて、初期の抽出物を得ること;
初期の抽出物から固形物を除去して、第2の初期の抽出物を得ること;
第2の初期の抽出物のpHを約4〜約7のpHに調節して、第1のpH調節された、第2の初期の抽出物を得ること;
第1のpH調節された、第2の初期の抽出物を酢酸エチルと接触させて、第1の酢酸エチル抽出物及び第2の水性抽出物を得ること;
第2の水性抽出物のpHを2未満のpHに調節して、pH調節された第2の水性抽出物を得ること;
pH調節された第2の水性抽出物を酢酸エチルと接触させて、第2の酢酸エチル抽出物を得ること;
酢酸エチルを第2の酢酸エチル抽出物から除去して、精製された組成物を得ること;
粗製組成物を水で再構成して、第3の水性抽出物を得ること;及び
第3の水性抽出物を脱色して、脱色された水性抽出物を得ること。「精製された組成物」が、初期の抽出物及び第2の初期の抽出物中のある程度の不純物を含まないことになるが、イェルバマテバイオマスに天然に存在するか、または本明細書に記載されているステップの1つ以上で生じ、脱色ステップにおいて除去される色の濃い高分子物質を含むという点で、「精製された組成物」は、対象の化合物(例えば、1つ以上のステビオール配糖体安定化化合物、及びその塩)を含むことになり、少なくとも初期の抽出物及び第2の初期の抽出物と比べて精製されている。
イェルバマテバイオマスを水性組成物と接触させて、初期の抽出物を得ること;
初期の抽出物から固形物を除去して、第2の初期の抽出物を得ること;
第2の初期の抽出物のpHを約2未満のpHに調節して、第2のpH調節された第2の初期の抽出物を得ること;
第2のpH調節された第2の初期の抽出物を酢酸エチルと接触させて、第3の酢酸エチル抽出物を得ること;
第3の酢酸エチル抽出物を中和して、第1の中和された酢酸エチル抽出物及び第3の水性抽出物を得ること;及び
第3の水性抽出物を脱色して、脱色された水性抽出物を得ること。
サンプル1:1mLの水中に10mgのReb M − H2O中で加熱される
サンプル2:1mLの水中に10mgのSE − H2O中で加熱される
サンプル3:1mLの水中に10mgのReb M+10mgのSE − H2O中で加熱される
サンプル4:3mLの水中の10mgのReb M+10mgのSE − H2O中で加熱されない
ステビオール配糖体と、
前記ステビオール配糖体の分解を低減するのに有効な量のステビオール配糖体安定化化合物と、
を含む組成物であって、前記ステビオール配糖体安定化化合物が、
カフェー酸、カフェー酸のエステル、カフェー酸とキナ酸のエステル、1つのカフェー酸部分を含むカフェー酸とキナ酸のエステル(例えば、クロロゲン酸、クリプトクロロゲン酸、及びネオクロロゲン酸;それぞれの構造が本明細書中で示されている)、2つ以上のカフェー酸部分を含むカフェー酸とキナ酸のエステル(例えば、1,3−ジカフェオイルキナ酸、1,4−ジカフェオイルキナ酸、1,5−ジカフェオイルキナ酸、3,4−ジカフェオイルキナ酸、3,5−ジカフェオイルキナ酸、及び4,5−ジカフェオイルキナ酸;それぞれの構造が本明細書中で示されている);
フェルラ酸、フェルラ酸のエステル、フェルラ酸とキナ酸のエステル、1つのフェルラ酸部分を含むフェルラ酸とキナ酸のエステル、2つ以上のフェルラ酸部分を含むフェルラ酸とキナ酸のエステル;
3−(3,4−ジヒドロキシフェニル)乳酸、3−(3,4−ジヒドロキシフェニル)乳酸誘導体、3−(3,4−ジヒドロキシフェニル)乳酸のエステル、3−(3,4−ジヒドロキシフェニル)乳酸誘導体のエステル、
キナ酸、キナ酸誘導体、キナ酸のエステル、キナ酸誘導体のエステル;
p−クマル酸、p−クマル酸のエステル、p−クマル酸とキナ酸のエステル、1つのp−クマル酸部分を含むp−クマル酸とキナ酸のエステル、2つ以上のp−クマル酸部分を含むp−クマル酸とキナ酸のエステル;
シナピン酸、シナピン酸のエステル、シナピン酸とキナ酸のエステル、1つのシナピン酸部分を含むシナピン酸とキナ酸のエステル、2つ以上のシナピン酸部分を含むシナピン酸とキナ酸のエステル;
酒石酸、酒石酸誘導体、酒石酸のエステル、酒石酸誘導体のエステル、及び
3−O−フェルロイルキナ酸、4−O−フェルロイルキナ酸、5−O−フェルロイルキナ酸、3,4−ジフェルロイルキナ酸、3,5−ジフェルロイルキナ酸、4,5−ジフェルロイルキナ酸からなる群から選択される少なくとも1つの化合物、及びその異性体である、組成物に関する。
Claims (14)
- ステビオール配糖体と、
前記ステビオール配糖体の分解を低減するのに有効な量のステビオール配糖体安定化化合物と、
を含む組成物であって、前記ステビオール配糖体安定化化合物は、
カフェー酸、カフェー酸のエステル、カフェー酸とキナ酸のエステル、1つのカフェー酸部分を含むカフェー酸とキナ酸のエステル(例えば、クロロゲン酸、クリプトクロロゲン酸、及びネオクロロゲン酸;それぞれの構造が本明細書中で示されている)、2つ以上のカフェー酸部分を含むカフェー酸とキナ酸のエステル(例えば、1,3−ジカフェオイルキナ酸、1,4−ジカフェオイルキナ酸、1,5−ジカフェオイルキナ酸、3,4−ジカフェオイルキナ酸、3,5−ジカフェオイルキナ酸、及び4,5−ジカフェオイルキナ酸;それぞれの構造が本明細書中で示されている);
フェルラ酸、フェルラ酸のエステル、フェルラ酸とキナ酸のエステル、1つのフェルラ酸部分を含むフェルラ酸とキナ酸のエステル、2つ以上のフェルラ酸部分を含むフェルラ酸とキナ酸のエステル;
3−(3,4−ジヒドロキシフェニル)乳酸、3−(3,4−ジヒドロキシフェニル)乳酸誘導体、3−(3,4−ジヒドロキシフェニル)乳酸のエステル、3−(3,4−ジヒドロキシフェニル)乳酸誘導体のエステル、
キナ酸、キナ酸誘導体、キナ酸のエステル、キナ酸誘導体のエステル;
p−クマル酸、p−クマル酸のエステル、p−クマル酸とキナ酸のエステル、1つのp−クマル酸部分を含むp−クマル酸とキナ酸のエステル、2つ以上のp−クマル酸部分を含むp−クマル酸とキナ酸のエステル;
シナピン酸、シナピン酸のエステル、シナピン酸とキナ酸のエステル、1つのシナピン酸部分を含むシナピン酸とキナ酸のエステル、2つ以上のシナピン酸部分を含むシナピン酸とキナ酸のエステル;
酒石酸、酒石酸誘導体、酒石酸のエステル、酒石酸誘導体のエステル、及び
3−O−フェルロイルキナ酸、4−O−フェルロイルキナ酸、5−O−フェルロイルキナ酸、3,4−ジフェルロイルキナ酸、3,5−ジフェルロイルキナ酸、4,5−ジフェルロイルキナ酸からなる群から選択される少なくとも1つの化合物、及びその異性体である、前記組成物。 - 前記組成物は、水性組成物である、請求項1に記載の組成物。
- 前記ステビオール配糖体の分解を低減するのに有効なステビオール配糖体安定化化合物の量は、安定化された前記ステビオール配糖体組成物が5%リン酸中、40℃で7日間保存される場合に、最初のステビオール配糖体のうちの少なくとも約10重量%が残っているような量である、請求項1に記載の組成物。
- 前記ステビオール配糖体は、少なくとも約0.03重量%のステビオール配糖体を含む、請求項1に記載の組成物。
- 前記ステビオール配糖体は、少なくとも約0.6重量%のステビオール配糖体を含む、請求項1に記載の組成物。
- 前記組成物は、1:0.3〜1:3の重量比のステビオール配糖体対ステビオール配糖体安定化化合物を含む、請求項1に記載の組成物。
- 前記組成物は、約4未満のpHを有する、請求項1に記載の組成物。
- 前記組成物は、約1未満のpHを有する、請求項1に記載の組成物。
- 前記組成物は、室温で保存される、請求項1に記載の組成物。
- 前記組成物は、4℃で保存される、請求項1に記載の組成物。
- 前記ステビオール配糖体は、レバウジオシドAまたはレバウジオシドMである、請求項1に記載の組成物。
- 前記ステビオール配糖体は、約1,800ppm〜約10,000ppmの間のステビオール配糖体を含む、請求項1に記載の組成物を含む飲料濃縮物製品。
- 前記ステビオール配糖体は、約1.5重量%〜約3.5重量%の間のステビオール配糖体を含む、請求項1に記載の組成物を含むリキットウォーターエンハンサー製品。
- 前記ステビオール配糖体は、約1.0重量%〜約10重量%の間のステビオール配糖体を含む、請求項1に記載の組成物を含む液体甘味料。
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US62/676,722 | 2018-05-25 | ||
PCT/US2018/054696 WO2019071187A1 (en) | 2017-10-06 | 2018-10-05 | STABILIZED GLYCOSIDE COMPOSITIONS OF STEVIOL AND USES THEREOF |
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