US20040259247A1 - Rna interference mediating small rna molecules - Google Patents

Rna interference mediating small rna molecules Download PDF

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Publication number
US20040259247A1
US20040259247A1 US10/433,050 US43305004A US2004259247A1 US 20040259247 A1 US20040259247 A1 US 20040259247A1 US 43305004 A US43305004 A US 43305004A US 2004259247 A1 US2004259247 A1 US 2004259247A1
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Prior art keywords
target
rna
gene
cell
sirna
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US10/433,050
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English (en)
Inventor
Thomas Tuschl
Sayda Elbashir
Winfried Lendeckel
Matthias Wilm
Reinhard Luhrmann
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Massachusetts Institute of Technology
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Europaisches Laboratorium fuer Molekularbiologie EMBL
Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
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Priority claimed from PCT/US2001/010188 external-priority patent/WO2001075164A2/en
Application filed by Europaisches Laboratorium fuer Molekularbiologie EMBL, Max Planck Gesellschaft zur Foerderung der Wissenschaften eV filed Critical Europaisches Laboratorium fuer Molekularbiologie EMBL
Priority to US10/433,050 priority Critical patent/US20040259247A1/en
Assigned to MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN E. V., EUROPAISCHES LABORATORIUM FUR MOLEKULARBIOLOGIE (EMBL) reassignment MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN E. V. ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: TUSCHL, THOMAS, WILM, MATTHIAS, LUHRMANN, REINHARD
Priority to US10/832,257 priority patent/US20050026278A1/en
Publication of US20040259247A1 publication Critical patent/US20040259247A1/en
Priority to US11/142,865 priority patent/US20050234006A1/en
Priority to US11/142,866 priority patent/US20050234007A1/en
Priority to US11/634,138 priority patent/US20080269147A1/en
Priority to US11/634,129 priority patent/US20070093445A1/en
Priority to US12/260,443 priority patent/US20090155174A1/en
Assigned to MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN. E.V. reassignment MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN. E.V. ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: ELBASHIR, SAYDA, LENDECKEL, WINFRIED
Priority to US12/537,632 priority patent/US20100010207A1/en
Priority to US12/537,602 priority patent/US8372968B2/en
Assigned to MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V. reassignment MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V. ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: EUROPAEISCHES LABORATORIUM FUER MOLEKULARBIOLOGIE (EMBL)
Priority to US12/591,829 priority patent/US8853384B2/en
Priority to US12/683,070 priority patent/US8933044B2/en
Priority to US12/683,081 priority patent/US8362231B2/en
Priority to US12/794,071 priority patent/US8765930B2/en
Priority to US12/819,444 priority patent/US8796016B2/en
Priority to US12/834,311 priority patent/US8445237B2/en
Priority to US12/835,086 priority patent/US8778902B2/en
Priority to US12/838,786 priority patent/US8329463B2/en
Priority to US12/879,300 priority patent/US8993745B2/en
Priority to US12/897,374 priority patent/US8895718B2/en
Assigned to UNIVERSITY OF MASSACHUSETTS, MASSACHUSETTS INSTITUTE OF TECHNOLOGY, WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH reassignment UNIVERSITY OF MASSACHUSETTS ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN E.V.
Assigned to UNIVERSITY OF MASSACHUSETTS, MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN E.V., WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH, MASSACHUSETTS INSTITUTE OF TECHNOLOGY reassignment UNIVERSITY OF MASSACHUSETTS CORRECTIVE ASSIGNMENT TO CORRECT THE OMISSION OF ASSIGNEE, MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN E.V., PREVIOUSLY RECORDED ON REEL 026583 FRAME 0614. ASSIGNOR(S) HEREBY CONFIRMS THE ASSIGNMENT. Assignors: MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN E.V.
Priority to US13/725,262 priority patent/US8895721B2/en
Priority to US14/476,465 priority patent/US9567582B2/en
Priority to US15/388,681 priority patent/US10633656B2/en
Priority to US16/805,072 priority patent/US20200299693A1/en
Abandoned legal-status Critical Current

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Definitions

  • RNA interference was coined after the discovery that injection of dsRNA into the nematode C. elegans leads to specific silencing of genes highly homologous in sequence to the delivered dsRNA (Fire et al., 1998). RNAi was subsequently also observed in insects, frogs (Oelgeschlager et al., 2000), and other animals including mice (Svoboda et al., 2000; Wianny and Zernicka-Goetz, 2000) and is likely to also exist in human.
  • RNAi is closely linked to the post-transcriptional gene-silencing (PTGS) mechanism of co-suppression in plants and quelling in fungi (Catalanotto et al., 2000; Cogoni and Macino, 1999; Dalmay et al., 2000; Ketting and Plasterk, 2000; Mourrain et al., 2000; Smardon et al., 2000) and some components of the RNAi machinery are also necessary for post-transcriptional silencing by co-suppression (Catalanotto et al., 2000; Dernburg et al., 2000; Ketting and Plasterk, 2000).
  • PTGS post-transcriptional gene-silencing
  • transgenes can also lead to transcriptional gene silencing via RNA-directed DNA methylation of cytosines (see references in Wassenegger, 2000). Genomic targets as short as 30 bp are methylated in plants in an RNA-directed manner (Pelissier, 2000). DNA methylation is also present in mammals.
  • RNAi and co-suppression appear to be protection of the genome against invasion by mobile genetic elements such as retro-transposons and viruses which produce aberrant RNA or dsRNA in the host cell when they become active (Jensen et al, 1999; Ketting et al., 1999; Ratcliff et al., 1999; Tabara et al., 1999).
  • Specific mRNA degradation prevents transposon and virus replication although some viruses are able to overcome or prevent this process by expressing proteins that suppress PTGS (Lucy et al., 2000; Voinnet et al., 2000).
  • RNA molecules of similar size also accumulate in plant tissue that exhibits PTGS (Hamilton and Baulcombe, 1999).
  • siRNAs short interfering RNAs
  • the siRNAs may also be important tools for transcriptional modulating, e.g. silencing of mammalian genes by guiding DNA methylation.
  • the object underlying the present invention is to provide novel agents capable of mediating target-specific RNA interference or other target-specific nucleic acid modifications such as DNA methylation, said agents having an improved efficacy and safety compared to prior art agents.
  • each RNA strand has a length from 19-25, particularly from 19-23 nucleotides, wherein said RNA molecule is capable of mediating target-specific nucleic acid modifications, particularly RNA interference and/or DNA methylation.
  • at least one strand has a 3′-overhang from 1-5 nucleotides, more preferably from 1-3 nucleotides and most preferably 2 nucleotides.
  • the other strand may be blunt-ended or has up to 6 nucleotides 3′ overhang.
  • the RNA molecule is preferably a synthetic RNA molecule which is substantially free from contaminants occurring in cell extracts, e.g. from Drosophila embryos. Further, the RNA molecule is preferably substantially free from any non-target-specific contaminants, particularly non-target-specific RNA molecules e.g. from contaminants occuring in cell extracts.
  • the invention relates to the use of isolated double-stranded RNA molecules, wherein each RNA strand has a length from 19-25 nucleotides, for mediating, target-specific nucleic acid modifications, particularly RNAi, in mammalian cells, particularly in human cells.
  • each strand of the RNA molecule has a length from 20-22 nucleotides (or 20-25 nucleotides in mammalian cells), wherein the length of each strand may be the same or different.
  • the length of the 3′-overhang reaches from 1-3 nucleotides, wherein the length of the overhang may be the same or different for each strand.
  • the RNA-strands preferably have 3′-hydroxyl groups.
  • the 5′-terminus preferably comprises a phosphate, diphosphate, triphosphate or hydroxyl group.
  • the most effective dsRNAs are composed of two 21 nt strands which are paired such that 1-3, particularly 2 nt 3′ overhangs are present on both ends of the dsRNA.
  • the target RNA cleavage reaction guided by siRNAs is highly sequence-specific. However, not all positions of a siRNA contribute equally to target recognition. Mismatches in the center of the siRNA duplex are most critical and essentially abolish target RNA cleavage. In contrast, the 3′ nucleotide of the siRNA strand (e.g. position 21) that is complementary to the single-stranded target RNA, does not contribute to specificity of the target recognition. Further, the sequence of the unpaired 2-nt 3′ overhang of the siRNA strand with the same polarity as the target RNA is not critical for target RNA cleavage as only the antisense siRNA strand guides target recognition. Thus, from the single-stranded overhanging nucleotides only the penultimate position of the antisense siRNA (e.g. position 20) needs to match the targeted sense mRNA.
  • the double-stranded RNA molecules of the present invention exhibit a high in vivo stability in serum or in growth medium for cell cultures.
  • the 3′-overhangs may be stablized against degradation, e.g. they may be selected such that they consist of purine nucleotides, particularly adenosine or guanosine nucleotides.
  • substitution of pyrimidine nucleotides by modified analogues e.g. substitution of uridine 2 nt 3′ overhangs by 2′-deoxythymidine is tolerated and does not affect the efficiency of RNA interference.
  • the absence of a 2′ hydroxyl significantly enhances the nuclease resistance of the overhang in tissue culture medium.
  • the RNA molecule may contain at least one modified nucleotide analogue.
  • the nucleotide analogues may be located at positions where the target-specific activity, e.g. the RNAi mediating activity is not substantially effected, e.g. in a region at the 5′-end and/or the 3′-end of the double-stranded RNA molecule.
  • the overhangs may be stabilized by incorporating modified nucleotide analogues.
  • O- and N-alkylated nucleotides e.g. N6-methyl adenosine are suitable.
  • the 2′OH-group is replaced by a group selected from H, OR, R, halo, SH, SR, NH 2 , NHR, NR 2 or CN, wherein R is C 1 -C 6 alkyl, alkenyl or alkynyl and halo is F, Cl, Br or I.
  • R is C 1 -C 6 alkyl, alkenyl or alkynyl and halo is F, Cl, Br or I.
  • the phosphoester group connecting to adjacent ribonucleotides is replaced by a modified group, e.g. of phosphothioate group. It should be noted that the above modifications may be combined.
  • the sequence of the double-stranded RNA molecule of the present invention has to have a sufficient identity to a nucleic acid target molecule in order to mediate target-specific RNAi and/or DNA methylation.
  • the sequence has an identity of at least 50%, particularly of at least 70% to the desired target molecule in the double-stranded portion of the RNA molecule. More preferably, the identity is at least 85% and most preferably 100% in the double-stranded portion of the RNA molecule.
  • I is the identity in percent
  • n is the number of identical nucleotides in the double-stranded portion of the ds RNA and the target
  • L is the length of the sequence overlap of the double-stranded portion of the dsRNA and the target.
  • the identity of the double-stranded RNA molecule to the target sequence may also be defined including the 3′ overhang, particularly an overhang having a length from 1-3 nucleotides.
  • the sequence identity is preferably at least 50%, more preferably at least 70% and most preferably at least 85% to the target sequence.
  • the nucleotides from the 3′ overhang and up to 2 nucleotides from the 5′ and/or 3′ terminus of the double strand may be modified without significant loss of activity.
  • the double-stranded RNA molecule of the invention may be prepared by a method comprising the steps:
  • RNA molecules Methods of synthesizing RNA molecules are known in the art. In this context, it is particularly referred to chemical synthesis methods as described in Verma and Eckstein (1998).
  • the single-stranded RNAs can also be prepared by enzymatic transcription from synthetic DNA templates or from DNA plasmids isolated from recombinant bacteria.
  • phage RNA polymerases are used such as T7, T3 or SP6 RNA polymerase (Milligan and Uhlenbeck (1989)).
  • a further aspect of the present invention relates to a method of mediating target-specific nucleic acid modifications, particularly RNA interference and/or DNA methylation in a cell or an organism comprising the steps:
  • the contacting step (a) comprises introducing the double-stranded RNA molecule into a target cell, e.g. an isolated target cell, e.g. in cell culture, a unicellular microorganism or a target cell or a plurality of target cells within a multicellular organism.
  • a target cell e.g. an isolated target cell, e.g. in cell culture, a unicellular microorganism or a target cell or a plurality of target cells within a multicellular organism.
  • the introducing step comprises a carrier-mediated delivery, e.g. by liposomal carriers or by injection.
  • the method of the invention may be used for determining the function of a gene in a cell or an organism or even for modulating the function of a gene in a cell or an organism, being capable of mediating RNA interference.
  • the cell is preferably a eukaryotic cell or a cell line, e.g. a plant cell or an animal cell, such as a mammalian cell, e.g. an embryonic cell, a pluripotent stem cell, a tumor cell, e.g. a teratocarcinoma cell or a virus-infected cell.
  • the organism is preferably a eukaryotic organism, e.g. a plant or an animal, such as a mammal, particularly a human.
  • the target gene to which the RNA molecule of the invention is directed may be associated with a pathological condition.
  • the gene may be a pathogen-associated gene, e.g. a viral gene, a tumor-associated gene or an autoimmune disease-associated gene.
  • the target gene may also be a heterologous gene expressed in a recombinant cell or a genetically altered organism. By determinating or modulating, particularly, inhibiting the function of such a gene valuable information and therapeutic benefits in the agricultural field or in the medicine or veterinary medicine field may be obtained.
  • the dsRNA is usually administered as a pharmaceutical composition.
  • the administration may be carried out by known methods, wherein a nucleic acid is introduced into a desired target cell in vitro or in vivo.
  • Commonly used gene transfer techniques include calcium phosphate, DEAE-dextran, electroporation and microinjection and viral methods (Graham, F. L. and van der Eb, A. J. (1973) Virol. 52, 456; McCutchan, J. H. and Pagano, J. S. (1968), J. Natl. Cancer Inst. 41, 351; Chu, G. et al (1987), Nucl. Acids Res. 15, 1311; Fraley, R. et al. (1980), J. Biol. Chem.
  • the invention also relates to a pharmaceutical composition containing as an active agent at least one double-stranded RNA molecule as described above and a pharmaceutical carrier.
  • the composition may be used for diagnostic and for therapeutic applications in human medicine or in veterinary medicine.
  • the composition may be in form of a solution, e.g. an injectable solution, a cream, ointment, tablet, suspension or the like.
  • the composition may be administered in any suitable way, e.g. by injection, by oral, topical, nasal, rectal application etc.
  • the carrier may be any suitable pharmaceutical carrier.
  • a carrier is used, which is capable of increasing the efficacy of the RNA molecules to enter the target-cells. Suitable examples of such carriers are liposomes, particularly cationic liposomes.
  • a further preferred administration method is injection.
  • a further subject matter of the invention is a eukaryotic cell or a eukaryotic non-human organism exhibiting a target gene-specific knockout phenotype comprising an at least partially deficient expression of at least one endogeneous target gene wherein said cell or organism is transfected with at least one double-stranded RNA molecule capable of inhibiting the expression of at least one endogeneous target gene or with a DNA encoding at least one double stranded RNA molecule capable of inhibiting the expression of at least one endogeneous target gene.
  • the present invention allows a target-specific knockout of several different endogeneous genes due to the specificity of RNAi.
  • RNAi based knockout technologies the expression of an endogeneous target gene may be inhibited in a target cell or a target organism.
  • the endogeneous gene may be complemented by an exogeneous target nucleic acid coding for the target protein or a variant or mutated form of the target protein, e.g. a gene or a cDNA, which may optionally be fused to a further nucleic acid sequence encoding a detectable peptide or polypeptide, e.g. an affinity tag, particularly a multiple affinity tag.
  • Variants or mutated forms of the target gene differ from the endogeneous target gene in that they encode a gene product which differs from the endogeneous gene product on the amino acid level by substitutions, insertions and/or deletions of single or multiple amino acids.
  • the variants or mutated forms may have the same biological activity as the endogeneous target gene.
  • the variant or mutated target gene may also have a biological activity, which differs from the biological activity of the endogeneous target gene, e.g. a partially deleted activity, a completely deleted activity, an enhanced activity etc.
  • the complementation may be accomplished by coexpressing the polypeptide encoded by the exogeneous nucleic acid, e.g. a fusion protein comprising the target protein and the affinity tag and the double stranded RNA molecule for knocking out the endogeneous gene in the target cell.
  • This coexpression may be accomplished by using a suitable expression vector expressing both the polypeptide encoded by the exogeneous nucleic acid, e.g. the tag-modified target protein and the double stranded RNA molecule or alternatively by using a combination of expression vectors. Proteins and protein complexes which are synthesized de novo in the target cell will contain the exogeneous gene product, e.g. the modified fusion protein.
  • the nucleotide sequence encoding the exogeneous nucleic acid may be altered on the DNA level (with or without causing mutations on the amino acid level) in the part of the sequence which is homologous to the double stranded RNA molecule.
  • the endogeneous target gene may be complemented by corresponding nucleotide sequences from other species, e.g. from mouse.
  • the method and cell of the invention are also suitable in a procedure for identifying and/or characterizing pharmacological agents, e.g. identifying new pharmacological agents from a collection of test substances and/or characterizing mechanisms of action and/or side effects of known pharmacological agents.
  • test substance or a collection of test substances wherein pharmacological properties of said test substance or said collection are to be identified and/or characterized.
  • the preparative method may be employed for the purification of high molecular weight protein complexes which preferably have a mass of ⁇ 150 kD and more preferably of ⁇ 500 kD and which optionally may contain nucleic acids such as RNA.
  • heterotrimeric protein complex consisting of the 20 kD, 60 kD and 90 kD proteins of the U4/U6 snRNP particle, the splicing factor SF3b from the 17S U2 snRNP consisting of 5 proteins having molecular weights of 14, 49, 1 20, 145 and 155 kD and the 25S U41U6/U5 tri-snRNP particle containing the U4, U5 and U6 snRNA molecules and about 30 proteins, which has a molecular weight of about 1.7 MD.
  • FIG. 2 A 29 bp dsRNA is no longer processed to 21-23 nt fragments.
  • FIG. 4 21 and 22 nt RNA fragments are generated by an RNase III-like mechanism.
  • RNAs were generated by incubation of internally radiolabeled 504 bp Pp-luc dsRNA in Drosophila lysate, gel-purified, and then digested to mononucleotides with nuclease P1 (top row) or ribonuclease T2 (bottom row).
  • the dsRNA was internally radiolabeled by transcription in the presence of one of the indicated ⁇ - 32 P nucleoside triphosphates. Radioactivity was detected by phosphorimaging.
  • Nucleoside 5′-monophosphates, nucleoside 3′-mono-phosphates, nucleoside 5′,3′-diphosphates, and inorganic phosphate are indicated as pN, Np, pNp, and p i , respectively.
  • Black circles indicate UV-absorbing spots from non-radioactive carrier nucleotides.
  • the 3′,5′-bis-phosphates (red circles) were identified by co-migration with radiolabeled standards prepared by 5′-phosphorylation of nucleoside 3′-mono-phosphates with T4 polynucleotide kinase and ⁇ - 32 P-ATP.
  • the sense strand of 21 and 22 nt short interfering RNAs (siRNAs) is shown blue, the antisense strand in red.
  • the sequences of the siRNAs were derived from the cloned fragments of 52 and 111 bp dsRNAs (FIG. 4A), except for the 22 nt antisense strand of duplex 5.
  • the siRNAs in duplex 6 and 7 were unique to the 111 bp dsRNA processing reaction.
  • the two 3′ overhanging nucleotides indicated in green are present in the sequence of the synthetic antisense strand of duplexes 1 and 3.
  • the stable 5′ cleavage products were resolved on the gel.
  • the cleavage sites are indicated in FIG. 5A.
  • the region targeted by the 52 bp dsRNA or the sense (s) or antisense (as) strands are indicated by the black bars to the side of the gel.
  • the cleavage sites are all located within the region of identity of the dsRNAs. For precise determination of the cleavage sites of the antisense strand, a lower percentage gel was used.
  • RNAi is predicted to begin with processing of dsRNA (sense strand in black, antisense strand in red) to predominantly 21 and 22 nt short interfering RNAs (siRNAs). Short overhanging 3′ nucleotides, if present on the dsRNA, may be beneficial for processing of short dsRNAs.
  • the dsRNA-processing proteins which remain to be characterized, are represented as green and blue ovals, and assembled on the dsRNA in asymmetric fashion. In our model, this is illustrated by binding of a hypothetical blue protein or protein domain with the siRNA strand in 3′ to 5′ direction while the hypothetical green protein or protein domain is always bound to the opposing siRNA strand.
  • siRNA duplex proteins remain associated with the siRNA duplex and preserve its orientation as determined by the direction of the dsRNA processing reaction. Only the siRNA sequence associated with the blue protein is able to guide target RNA cleavage.
  • the endonuclease complex is referred to as small interfering ribonucleoprotein complex or siRNP. It is presumed here, that the endonuclease that cleaves the dsRNA may also cleave the target RNA, probably by temporarily displacing the passive siRNA strand not used for target recognition. The target RNA is then cleaved in the center of the region recognized by the sequence-complementary guide siRNA.
  • FIG. 8 Reporter constructs and siRNA duplexes.
  • the region targeted by the siRNA duplexes is indicated as black bar below the coding region of the luciferase genes.
  • the sense (top) and antisense (bottom) sequences of the siRNA duplexes targeting GL2, GL3 and RL luciferase are shown.
  • the GL2 and GL3 siRNA duplexes differ by only 3 single nucleotide substitutions (boxed in gray).
  • a duplex with the inverted GL2 sequence, invGL2 was synthesized.
  • the 2 nt 3′ overhang of 2′-deoxythymidine is indicated as TT; uGL2 is similar to GL2 siRNA but contains ribo-uridine 3′ overhangs.
  • FIG. 9 RNA interference by siRNA duplexes.
  • Ratios of target control luciferase were normalized to a buffer control (bu, black bars); gray bars indicate ratios of Photinus pyralis (Pp-luc) GL2 or GL3 luciferase to Renilla reniformis (Rr-luc) RL luciferase (left axis), white bars indicate RL to GL2 or GL3 ratios (right axis).
  • Panels a, c, e, g and i describe experiments performed with the combination of pGL2-Control and pRL-TK reporter plasmids, panels b, d, f, h and j with pGL3-Control and pRL-TK reporter plasmids.
  • the cell line used for the interference experiment is indicated at the top of each plot.
  • the ratios of Pp-luc/Rr-luc for the buffer control (bu) varied between 0.5 and 10 for pGL2/pRL and between 0.03 and 1 for pGL3/pRL, respectively, before normalization and between the various cell lines tested.
  • the plotted data were averaged from three independent experiments ⁇ S.D.
  • FIG. 10 Effects of 21 nt siRNA, 50 bp and 500 bp dsRNAs on luciferase expression in HeLa cells.
  • the ratios of luciferase activity for siRNA duplexes were normalized to a buffer control (bu, black bars); the luminescence ratios for 50 or 500 bp dsRNAs were normalized to the respective ratios observed for 50 and 500 bp dsRNA from humanized GFP (hG, black bars). It should be noted that the overall differences in sequences between the 49 and 484 bp dsRNAs targeting GL2 and GL3 are not sufficient to confer specificity between GL2 and GL3 targets (43 nt uninterrupted identity in 49 bp segment, 239 nt longest uninterrupted identity in 484 bp segment).
  • FIG. 11 Variation of the 3′ overhang of duplexes of 21-nt siRNAs.
  • the luminescence ratios determined in the presence of dsRNA were normalized to the ratio obtained for a buffer control (bu, black bar). Normalized ratios less than 1 indicate specific interference.
  • C-J Normalized interference ratios for eight series of 21-nt siRNA duplexes. The sequences of siRNA duplexes are depicted above the bar graphs. Each panel shows the interference ratio for a set of duplexes formed with a given antisense guide siRNA and 5 different sense siRNAs. The number of overhanging nucleotides (3′ overhang, positive numbers; 5′ overhangs, negative numbers) is indicated on the x-axis. Data points were averaged from at least 3 independent experiments, error bars represent standard deviations.
  • FIG. 12 Variation of the length of the sense strand of siRNA duplexes.
  • FIG. 13 Variation of the length of siRNA duplexes with preserved 2-nt 3′ overhangs.
  • FIG. 11H or 12 C The siRNA duplexes were extended to the 3′ side of the sense siRNA (B) or the 5′ side of the sense siRNA (C). The siRNA duplex sequences and the respective interference ratios are indicated.
  • FIG. 14 Substitution of the 2′-hydroxyl groups of the siRNA ribose residues.
  • FIG. 15 Mapping of sense and antisense target RNA cleavage by 21-nt siRNA duplexes with 2-nt 3′ overhangs.
  • the antisense siRNA was the same in all siRNA duplexes, but the sense strand was varied between 18 to 25 nt by changing the 3′ end (A) or 18 to 23 nt by changing the 5′ end (B).
  • the position of sense and antisense target RNA cleavage is indicated by triangles on top and below the siRNA duplexes, respectively.
  • C, D Analysis of target RNA cleavage using cap-labelled sense (top panel) or antisense (bottom panel) target RNAs. Only the cap-labelled 5′ cleavage products are shown. The sequences of the siRNA duplexes are indicated, and the length of the sense siRNA strands is marked on top of the panel.
  • the control lane marked with a dash in panel (C) shows target RNA incubated in absence of siRNAs. Markers were as described in FIG. 15.
  • the arrows in (D), bottom panel, indicate the target RNA cleavage sites that differ by 1 nt.
  • FIG. 17 Sequence variation of the 3′ overhang of siRNA duplexes.
  • the 2-nt 3′ overhang (NN, in gray) was changed in sequence and composition as indicated (T, 2′-deoxythymidine, dG, 2′-deoxyguanosine; asterisk, wild-type siRNA duplex). Normalized interference ratios were determined as described in FIG. 11. The wild-type sequence is the same as depicted in FIG. 14.
  • FIG. 18 Sequence specificity of target recognition.
  • the sequences of the mismatched siRNA duplexes are shown, modified sequence segments or single nucleotides are underlayed in gray.
  • the reference duplex (ref) and the siRNA duplexes 1 to 7 contain 2′-deoxythymidine 2-nt overhangs.
  • the silencing efficiency of the thymidine-modified reference duplex was comparable to the wild-type sequence (FIG. 17). Normnalized interference ratios were determined as described in FIG. 11.
  • FIG. 19 Variation of the length of siRNA duplexes with preserved 2-nt 3′ overhangs.
  • siRNA duplexes were extended to the 3′ side of the sense siRNA (A) or the 5′ side of the sense siRNA (B).
  • the siRNA duplex sequences and the respective interference ratios are indicated.
  • siRNA duplexes (0.84 ⁇ g) targeting GL2 luciferase were transfected together with pGL2-Control and pRL-TK plasmids.
  • the in vitro RNAi activities of siRNA duplexes tested in D. melanogaster lysate are indicated.
  • RNAi translation assays were performed with dsRNA concentrations of 5 nM and an-extended pre-incubation period of 15 min at 25° C. prior to the addition of in vitro transcribed, capped and polyadenylated Pp-luc and Rr-luc reporter mRNAs. The incubation was continued for 1 h and the relative amount of Pp-luc and Rr-luc protein was analyzed using the dual luciferase assay (Promega) and a Monolight 3010C luminometer (PharMingen).
  • RNA was prepared using Expedite RNA phosphoramidites (Proligo).
  • the 3′ adapter oligonucleotide was synthesized using dimethoxytrityl-1,4-benzenedimethanol-succinyl-aminopropyl-CPG.
  • the oligoribonucleotides were deprotected in 3 ml of 32% ammonia/ethanol (3/1) for 4 h at 55° C. (Expedite RNA) or 16 h at 55° C.
  • RNA transcripts for dsRNA preparation including long 3′ overhangs were generated from PCR templates that contained a T7 promoter in sense and an SP6 promoter in antisense direction.
  • the transcription template for sense and antisense target RNA was PCR-amplified with GCG TAATACGACTCACTATA GAACAATTGCTTTTACAG (underlined, T7 promoter) as 5′ primer and ATTTAGGTGACACTATA GGCATAAAGAATTGAAGA (underlined, SP6 promoter) as 3′ primer and the linearized Pp-luc plasmid (pGEM-luc sequence) (Tuschl et al., 1999) as template; the T7-transcribed sense RNA was 177 nt long with the Pp-luc sequence between pos. 113-273 relative to the start codon and followed by 17 nt of the complement of the SP6 promoter sequence at the 3′ end. Transcripts for blunt-ended dsRNA formation were prepared by transcription from two different PCR products which only contained a single promoter sequence.
  • DsRNA annealing was carried out using a phenol/chloroform extraction.
  • Equimolar concentration of sense and antisense RNA 50 nM to 10 ⁇ M, depending on the length and amount available
  • 0.3 M NaOAc pH 6
  • the resulting dsRNA was precipitated by addition of 2.5-3 volumes of ethanol.
  • the pellet was dissolved in lysis buffer (100 mM KCl, 30 mM HEPES-KOH, pH 7.4, 2 mM Mg(OAc) 2 ) and the quality of the dsRNA was verified by standard agarose gel electrophoreses in 1 ⁇ TAE-buffer.
  • the 52 bp dsRNAs with the 17 nt and 20 nt 3′ overhangs (FIG. 6) were annealed by incubating for 1 min at 95 ° C., then rapidly cooled to 70° C. and followed by slow cooling to room temperature over a 3 h period (50 ⁇ l annealing reaction, 1 ⁇ M strand concentration, 300 mM NaCl, 10 mM Tris-HCl, pH 7.5).
  • the dsRNAs were then phenol/chloroform extracted, ethanol-precipitated and dissolved in lysis buffer.
  • RNA used for dsRNA preparation was performed using 1 mM ATP, CTP, GTP, 0.1 or 0.2 mM UTP, and 0.2-0.3 ⁇ M- 32 P-UTP (3000 Ci/mmol), or the respective ratio for radiolabeled nucleoside triphosphates other than UTP. Labeling of the cap of the target RNAs was performed as described previously. The target RNAs were gel-purified after cap-labeling.
  • RNAi reactions were performed by pre-incubating 10 nM dsRNA for 15 min followed by addition of 10 nM cap-labeled target RNA. The reaction was stopped after a further 2 h (FIG. 2A) or 2.5 h incubation (FIGS. 5B and 6B) by proteinase K treatment (Tuschl et al., 1999). The samples were then analyzed on 8 or 10% sequencing gels. The 21 and 22 nt synthetic RNA duplexes were used at 100 nM final concentration (FIG. 5B).
  • the 21 nt RNAs were produced by incubation of radiolabeled dsRNA in Drosophila lysate in absence of target RNA (200 ⁇ l reaction, 1 h incubation, 50 nM dsP111, or 100 nM dsP52 or dsP39).
  • the reaction mixture was subsequently treated with proteinase K (Tuschl et al., 1999) and the dsRNA-processing products were separated on a denaturing 15% polyacrylamide gel.
  • A-band including a size range of at least 18 to 24 nt, was excised, eluted into 0.3 M NaCl overnight at 4° C. and in siliconized tubes.
  • RNA was recovered by ethanol-precipitation and dephosphorylated (30 ⁇ l reaction, 30 min, 50° C., 10 U alkaline phosphatase, Roche). The reaction was stopped by phenol/chloroform extraction and the RNA was ethanol-precipitated.
  • the 3′ adapter oligonucleotide (pUUUaaccgcatccttctcx: uppercase, RNA; lowercase, DNA; p, phosphate; x, 4-hydroxymethylbenzyl) was then ligated to the dephosphorylated ⁇ 21 nt RNA (20 ⁇ l reaction, 30 min, 37° C., 5 ⁇ M 3′ adapter, 50 mM Tris-HCl, pH 7.6, 10 mM MgCl 2 , 0.2 mM ATP, 0.1 mg/ml acetylated BSA, 15% DMSO, 25 U T4 RNA ligase, Amersham-Pharmacia) (Pan and Uhlenbeck, 1992).
  • the ligation reaction was stopped by the addition of an equal volume of 8 M urea/50 mM EDTA stopmix and directly loaded on a 15% gel. Ligation yields were greater 50%.
  • the ligation product was recovered from the gel and 5′-phosphorylated (20 ⁇ l reaction, 30 min, 37° C., 2 mM ATP, 5 U T4 polynucleotide kinase, NEB).
  • the phosphorylation reaction was stopped by phenol/chloroform extraction and RNA was recovered by ethanol-precipitation.
  • the 5′ adapter (tactaatacgactcactAAA: uppercase, RNA; lowercase, DNA) was ligated to the phosphorylated ligation product as described above.
  • the new ligation product was gel-purified and eluted from the gel slice in the presence of reverse transcription primer (GACTAGCTGGAATTCAAGGATGCGGTTAAA: bold, Eco RI site) used as carrier.
  • Reverse transcription (15 ⁇ l reaction, 30 min, 42° C., 150 U Superscript 11 reverse transcriptase, Life Technologies) was followed by PCR using as 5′ primer CAGCCAACGGAATTCATACGACTCACTAAA (bold, Eco RI site) and the 3′ RT primer.
  • the PCR product was purified by phenol/chloroform extraction and ethanol-precipitated.
  • PCR product was then digested with Eco RI (NEB) and concatamerized using T4 DNA ligase (high conc., NEB). Concatamers of a size range of 200 to 800 bp were separated on a low-melt agarose gel, recovered from the gel by a standard melting and phenol extraction procedure, and ethanol-precipitated. The unpaired ends were filled in by incubation with Taq polymerase under standard conditions for 1 5 min at 72° C. and the DNA product was directly ligated into the pCR2.1-TOPO vector using the TOPO TA cloning kit (Invitrogen). Colonies were screened using PCR and M13-20 and M13 Reverse sequencing primers. PCR products were directly submitted for custom sequencing (Sequence Laboratories Göttingen GmbH, Germany). On average, four to five 21 mer sequences were obtained per clone.
  • Nuclease P1 digestion of radiolabeled, gel-purified siRNAs and 2D-TLC was carried out as described (Zamore et al., 2000). Nuclease T2 digestion was performed in 10 ⁇ l reactions for 3 h at 50° C. in 10 mM ammonium acetate (pH 4.5) using 2 ⁇ g/ ⁇ l carrier tRNA and 30 U ribonuclease T2 (Life Technologies). The migration of non-radioactive standards was determined by UV shadowing.
  • nucleoside-3′,5′-disphosphates The identity of nucleoside-3′,5′-disphosphates was confirmed by co-migration of the T2 digestion products with standards prepared by 5′- 32 P-phosphorylation of commercial nucleoside 3′-monophosphates using ⁇ -32P-ATP and T4 polynucleotide kinase (data not shown).
  • Lysate prepared from D. melanogaster syncytial embryos recapitulates RNAi in vitro providing a novel tool for biochemical analysis of the mechanism of RNAi (Tuschl et al., 1999; Zamore et al., 2000).
  • In vitro and in vivo analysis of the length requirements of dsRNA for RNAi has revealed that short dsRNA ( ⁇ 1 50 bp) are less effective than longer dsRNAs in degrading target mRNA (Caplen et al., 2000; Hammond et al., 2000; Ngo et al., 1998); Tuschl et al., 1999). The reasons for reduction in mRNA degrading efficiency are not understood.
  • 21-23 nt RNA fragments generated by processing of dsRNAs are the mediators of RNA interference and co-suppression (Hamilton and Baulcombe, 1999; Hammond et al., 2000; Zamore et al., 2000).
  • Formation of 21-23 nt fragments in Drosophila lysate (FIG. 2) was readily detectable for 39 to 501 bp long dsRNAs but was significantly delayed for the 29 bp dsRNA.
  • the 52 bp dsRNA which shares the same 5′ end with the 39 bp dsRNA, produces the same cleavage site on the sense target, located 10 nt from the 5′ end of the region of identity with the dsRNA, in addition to two weaker cleavage sites 23 and 24 nt downstream of the first site.
  • the antisense target was only cleaved once, again 10 nt from the 5′ end of the region covered by its respective dsRNA. Mapping of the cleavage sites for the 38 to 49 bp dsRNAs shown in FIG. 1 showed that the first and predominant cleavage site was always located 7 to 10 nt downstream of the region covered by the dsRNA (data not shown). This suggests that the point of target RNA cleavage is determined by the end of the dsRNA and could imply that processing to 21-23 mers starts from the ends of the duplex.
  • Cleavage sites on sense and antisense target for the longer 111 bp dsRNA were much more frequent than anticipated and most of them appear in clusters separated by 20 to 23 nt (FIGS. 3A and 3B).
  • the first cleavage site on the sense target is 10 nt from the 5′ end of the region spanned by the dsRNA
  • the first cleavage site on the antisense target is located 9 nt from the 5′ end of region covered by the dsRNA.
  • RNAi in transposon silencing.
  • 3′ nucleotides that are derived from untemplated addition of nucleotides during RNA synthesis using T7 RNA polymerase.
  • endogenous Drosophila ⁇ 21 nt RNAs were also cloned, some of them from LTR and non-LTR retrotransposons (data not shown). This is consistent with a possible role for RNAi in transposon silencing.
  • the ⁇ 21 nt RNAs appear in clustered groups (FIG. 4A) which cover the entire dsRNA sequences. Apparently, the processing reaction cuts the dsRNA by leaving staggered 3′ ends, another characteristic of RNase III cleavage. For the 39 bp dsRNA, two clusters of ⁇ 21 nt RNAs were found from each dsRNA-constituting strand including overhanging 3′ ends, yet only one cleavage site was detected on the sense and antisense target (FIGS. 3A and 3B).
  • ⁇ 21 nt fragments were present as single-stranded guide RNAs in a complex that mediates mRNA degradation, it could be assumed that at least two target cleavage sites exist, but this was not the case. This suggests that the ⁇ 21 nt RNAs may be present in double-stranded form in the endonuclease complex but that only one of the strands can be used for target RNA recognition and cleavage.
  • the use of only one of the ⁇ 21 nt strands for target cleavage may simply be determined by the orientation in which the ⁇ 21 nt duplex is bound to the nuclease complex. This orientation is defined by the direction in which the original dsRNA was processed.
  • the ⁇ 21 mer clusters for the 52 bp and 111 bp dsRNA are less well defined when compared to the 39 bp dsRNA.
  • the clusters are spread over regions of 25 to 30 nt most likely representing several distinct subpopulations of ⁇ 21 nt duplexes and therefore guiding target cleavage at several nearby sites. These cleavage regions are still predominantly separated by 20 to. 23 nt intervals.
  • the rules determining how regular dsRNA can be processed to ⁇ 21 nt fragments are not yet understood, but it was previously observed that the approx. 21-23 nt spacing of cleavage sites could be altered by a run of uridines (Zamore et al., 2000).
  • dsRNA cleavage by E. coli RNase III appears to be mainly controlled by antideterminants, i.e. excluding some specific base-pairs at given positions relative to the cleavage site (Zhang and Nicholson, 1997).
  • nucleoside 3′,5′-diphosphates All four nucleoside 3′,5′-diphosphates were detected and suggest that the internucleotidic linkage was cleaved with little or no sequence-specificity.
  • the ⁇ 21 nt fragments are unmodified and were generated from dsRNA such that 5′-monophosphates and 3′-hydroxyls were present at the 5′-end.
  • the 21 and 22 nt RNA duplexes were incubated at 100 nM concentrations in the lysate, a 10-fold higher concentrations-than the 52 bp control dsRNA. Under these conditions, target RNA cleavage is readily detectable. Reducing the concentration of 21 and 22 nt duplexes from 100 to 10 nM does still cause target RNA cleavage. Increasing the duplex concentration from 100 nM to 1000 nM however does not further increase target cleavage, probably due to a limiting protein factor within the lysate.
  • the 21 and 22 nt dsRNAs with overhanging 3′ ends of 2 to 4 nt mediated efficient degradation of target RNA (duplexes 1, 3, 4, 6, FIGS. 5A and 5B).
  • Blunt-ended 21 or 22 nt dsRNAs (duplexes 2, 5, and 7, FIGS. 5A and 5B) were reduced in their ability to degrade the target and indicate that overhanging 3′ ends are critical for reconstitution of the RNA-protein nuclease complex.
  • the single-stranded overhangs may be required for high affinity binding of the ⁇ 21 nt duplex to the protein components.
  • the target cleavage site is located 11 or 1 2 nt downstream of the first nucleotide that is complementary to the 21 or 22 nt guide sequence, i.e. the cleavage site is near center of the region covered by the 21 or 22 nt RNAs (FIGS. 4A and 4B).
  • Displacing the sense strand of a 22 nt duplex by two nucleotides (compare duplexes 1 and 3 in FIG. 5A) displaced the cleavage site of only the antisense target by two nucleotides.
  • Displacing both sense and antisense strand by two nucleotides shifted both cleavage sites by two nucleotides (compare duplexes 1 and 4).
  • siRNAs short interfering RNAs
  • siRNP small interfering ribonucleoprotein particle
  • long dsRNAs may also contain internal dsRNA-processing signals or may get processed cooperatively due to the association of multiple cleavage factors.
  • Double-stranded RNA is first processed to short RNA duplexes of predominantly 21 and 22 nt in length and with staggered 3′ ends similar to an RNase III-like reaction (Dunn, 1982; Nicholson, 1999; Robertson, 1982). Based on the 21-23 nt length of the processed RNA fragments it has already been speculated that an RNase III-like activity may be involved in RNAi (Bass, 2000).
  • RNase III homologs Little is known about the biochemistry of RNase III homologs from plants, animals or human.
  • Two families of RNase III enzymes have been identified predominantly by database-guided sequence analysis or cloning of cDNAs.
  • the first RNase-III family is represented by the 1327 amino acid long D. melanogaster protein drosha (Acc. AF116572).
  • the C-terminus is composed of two RNase III and one dsRNA-binding domain and the N-terminus is of unknown function. Close homologs are also found in C. elegans (Acc. AF160248) and human (Acc. AF18901 1) (Filippov et al., 2000; Wu et al., 2000).
  • the drosha-like human RNase III was recently cloned and characterized (Wu et al., 2000).
  • the gene is ubiquitously expressed in human tissues and cell lines, and the protein is localized in the nucleus and the nucleolus of the cell. Based on results inferred from antisense inhibition studies, a role of this protein for rRNA processing was suggested.
  • the second class is represented by the C. elegans gene K12H4.8 (Acc. S44849) coding for a 1822 amino acid long protein.
  • This protein has an N-terminal RNA helicase motif which is followed by 2 RNase III catalytic domains and a dsRNA-binding motif, similar to the drosha RNase III family. There are close homologs in S.
  • pombe (Acc. Q09884), A. thaliana (Acc. AF187317), D. melanogaster (Acc. AE003740), and human (Acc. AB028449) (Filippov et al., 2000; Jacobsen et al., 1 999; Matsuda et al., 2000). Possibly the K12H4.8 RNase III/helicase is the likely candidate to be involved in RNAi.
  • Processing to the siRNA duplexes appears to start from the ends of both blunt-ended dsRNAs or dsRNAs with short (1-5 nt) 3′ overhangs, and proceeds in approximately 21-23 nt steps.
  • Long ( ⁇ 20 nt) 3′ staggered ends on short dsRNAs suppress RNAi, possibly through interaction with single-stranded RNA-binding proteins.
  • the suppression of RNAi by single-stranded regions flanking short dsRNA and the lack of siRNA formation from short 30 bp dsRNAs may explain why structured regions frequently encountered in mRNAs do not lead to activation of RNAi.
  • siRNA duplexes guide cleavage of sense as well as antisense target RNA as they are able to associate with the protein components in either of the two possible orientation.
  • siRNA duplexes represent a new alternative to antisense or ribozyme therapeutics.
  • RNAs were chemically synthesized using Expedite RNA phosphoramidites and thymidine phosphoramidite (Proligo, Germany). Synthetic oligonucleotides were deprotected and gel-purified (Example 1), followed by Sep-Pak C18 cartridge (Waters, Milford, Mass., USA) purification (Tuschl, 1993).
  • the siRNA sequences targeting GL2 (Acc. X65324) and GL3 luciferase (Acc. U47296) corresponded to the coding regions 153-173 relative to the first nucleotide of the start codon, siRNAs targeting RL (Acc.
  • AF025846 corresponded to region 119-129 after the start codon.
  • Longer RNAs were transcribed with T7 RNA polymerase from PCR products, followed by gel and Sep-Pak purification.
  • the 49 and 484 bp GL2 or GL3 dsRNAs corresponded to position 113-161 and 113-596, respectively, relative to the start of translation; the 50 and 501 bp RL dsRNAs corresponded to position 118-167 and 118-618, respectively.
  • PCR templates for dsRNA synthesis targeting humanized GFP were amplified from pAD3 (Kehlenbach, 1998), whereby 50 and 501 bp hG dsRNA corresponded to position 118-167 and 118-618, respectively, to the start codon.
  • annealing buffer 100 mM potassium acetate, 30 mM HEPES-KOH at pH 7.4, 2 mM magnesium acetate
  • annealing buffer 100 mM potassium acetate, 30 mM HEPES-KOH at pH 7.4, 2 mM magnesium acetate
  • the 37° C. incubation step was extended overnight for the 50 and 500 bp dsRNAs and these annealing reactions were performed at 8.4 ⁇ M and 0.84 ⁇ M strand concentrations, respectively.
  • S2 cells were propagated in Schneider's Drosophila medium (Life Technologies) supplemented with 10% FBS, 100 units/ml penicillin and 100 ⁇ g/ml streptomycin at 25° C. 293, NIH/3T3, HeLa S3, COS-7 cells were grown at 37° C. in Dulbecco's modified Eagle's medium supplemented with 10% FBS, 100 units/ml penicillin and 100 ⁇ g/ml streptomycin. Cells were regularly passaged to maintain exponential growth. 24 h before transfection at approx.
  • mammalian cells were trypsinized and diluted 1:5 with fresh medium without antibiotics (1-3 ⁇ 10 5 cells/ml) and transferred to 24-well plates (500 ⁇ l/well). S2 cells were not trypsinized before splitting. Transfection was carried out with Lipofectamine 2000 reagent (Life Technologies) as described by the manufacturer for adherent cell lines. Per well, 1.0 ⁇ g pGL2-Control (Promega) or pGL3-Control (Promega), 0.1 ⁇ g pRL-TK (Promega) and 0.28 ⁇ g siRNA duplex or dsRNA, formulated into liposomes, were applied; the final volume was 600 ⁇ l per well.
  • siRNA duplexes were co-transfected with the reporter plasmid combinations pGL2/pRL or pGL3/pRL into D. melanogaster Schneider S2 cells or mammalian cells using cationic liposomes. Luciferase activities were determined 20 h after transfection.
  • FIG. 9 a - j In all cell lines tested, we observed specific reduction of the expression of the reporter genes in the presence of cognate siRNA duplexes (FIG. 9 a - j ). Remarkably, the absolute luciferase expression levels were unaffected by non-cognate siRNAs, indicating the absence of harmful side effects by 21 nt RNA duplexes (e.g. FIG. 10 a - d for HeLa cells). In D. melanogaster S2 cells (FIG. 9 a, b ), the specific inhibition of luciferases was complete. In mammalian cells, where the reporter genes were 50- to 100-fold stronger expressed, the specific suppression was less complete (FIG. 9 c - j ).
  • GL2 expression was reduced 3- to 12-fold, GL3 expression 9- to 25-fold and RL expression 1- to 3-fold, in response to the cognate siRNAs.
  • targeting of RL luciferase by RL siRNAs was ineffective, although GL2 and GL3 targets responded specifically (FIG. 9 i, j ).
  • the lack of reduction of RL expression in 293 cells may be due to its 5- to 20-fold higher expression compared to any other mammalian cell line tested and/or to limited accessibility of the target sequence due to RNA secondary structure or associated proteins. Nevertheless, specific targeting of GL2 and GL3 luciferase by the cognate siRNA duplexes indicated that RNAi is also functioning in 293 cells.
  • the thymidine overhang was chosen, because it is supposed to enhance nuclease resistance of siRNAs in the tissue culture medium and within transfected cells. Indeed, the thymidine-modified GL2 siRNA was slightly more potent than the unmodified uGL2 siRNA in all cell lines tested (FIG. 9 a, c, e, g, i ). It is conceivable that further modifications of the 3′ overhanging nucleotides may provide additional benefits to the delivery and stability of siRNA duplexes.
  • siRNA duplexes were used (FIG. 9, 10).
  • Increasing the siRNA concentration to 100 nM did not enhance the specific silencing effects, but started to affect transfection efficiencies due to competition for liposome encapsulation between plasmid DNA and siRNA (data not shown).
  • Decreasing the siRNA concentration to 1.5 nM did not reduce the specific silencing effect (data not shown), even though the siRNAs were now only 2- to 20-fold more concentrated than the DNA plasmids. This indicates that siRNAs are extraordinarily powerful reagents for mediating gene silencing and that siRNAs are effective at concentrations that are several orders of magnitude below the concentrations applied in conventional antisense or ribozyme gene targeting experiments.
  • dsRNAs In order to monitor the effect of longer dsRNAs on mammalian cells, 50 and 500 bp dsRNAs cognate to the reporter genes were prepared. As non-specific control, dsRNAs from humanized GFP (hG) (Kehlenbach, 1998) was used. When dsRNAs were co-transfected, in identical amounts (not concentrations) to the siRNA duplexes, the reporter gene expression was strongly and unspecifically reduced. This effect is illustrated for HeLa cells as a representative example (FIG. 10 a - d ).
  • the absolute luciferase activities were decreased unspecifically 10- to 20-fold by 50 bp dsRNA and 20- to 200-fold by 500 bp dsRNA co-transfection, respectively. Similar unspecific effects were observed for COS-7 and NIH/3T3 cells. For 293 cells, a 10- to 20-fold unspecific reduction was observed only for 500 bp dsRNAs. Unspecific reduction in reporter gene expression by dsRNA >30 bp was expected as part of the interferon response.
  • CHO-K1 cells appear to be deficient in the interferon response.
  • 293, NIH/3T3 and BHK-21 cells were tested for RNAi using luciferase/lacZ reporter combinations and 829 bp specific lacZ or 717 bp unspecific GFP dsRNA (Caplen, 2000).
  • the failure of detecting RNAi in this case may be due to the less sensitive luciferase/lacZ reporter assay and the length differences of target and control dsRNA.
  • our results indicate that RNAi is active in mammalian cells, but that the silencing effect is difficult to detect, if the interferon system is activated by dsRNA >30 bp.
  • siRNA-mediated gene silencing in mammalian cells.
  • the use of short siRNAs holds great promise for inactivation of gene function in human tissue culture and the development of gene-specific therapeutics.
  • RNA synthesis, annealing, and luciferase-based RNAi assays were performed as described in Examples 1 or 2 or in previous publications (Tuschl et al., 1999; Zamore et al., 2000). All siRNA duplexes were directed against firefly luciferase, and the luciferase mRNA sequence was derived from pGEM-luc (GenBank acc. X65316) as described (Tuschl et al., 1999). The siRNA duplexes were incubated in D. melanogaster RNAi/translation reaction for 15 min prior to addition of mRNAs. Translation-based RNAi assays were performed at least in triplicates.
  • mapping of sense target RNA cleavage a 177-nt transcript was generated, corresponding to the firefly luciferase sequence between positions 113-273 relative to the start codon, followed by the 17-nt complement of the SP6 promoter sequence.
  • mapping of antisense target RNA cleavage a 166-nt transcript was produced from a template, which was amplified from plasmid sequence by PCR using 5′ primer TAATACGACTCACTATAGA GCCCATATCGTTTCATA (T7 promoter underlined) and 3! primer AGAGGATGGAACCGCTGG.
  • the target sequence corresponds to the complement of the firefly luciferase sequence between positions 50-215 relative to the start codon.
  • Guanylyl transferase labelling was performed as previously described (Zamore et al., 2000).
  • 100 nM siRNA duplex was incubated with 5 to 10 nM target RNA in D. melanogaster embryo lysate under standard conditions (Zamore et al., 2000) for 2 h at 25° C.
  • the reaction was stopped by the addition of 8 volumes of proteinase K buffer (200 mM Tris-HCl pH 7.5, 25 mM EDTA, 300 mM NaCl, 2% w/v sodium dodecyl sulfate). Proteinase K (E.M.
  • siRNA duplexes As described above, 2 or 3 unpaired nucleotides at the 3′ end of siRNA duplexes were more efficient in target RNA degradation than the respective blunt-ended duplexes.
  • sense and antisense siRNAs eight series of siRNA duplexes with synthetic overhanging ends were generated covering a range of 7-nt 3′ overhang to 4-nt 5′ overhang.
  • siRNA duplexes The interference of siRNA duplexes was measured using the dual luciferase assay system (Tuschl et al., 1999; Zamore et al., 2000). siRNA duplexes were directed against firefly luciferase mRNA, and sea pansy luciferase mRNA was used as internal control. The luminescence ratio of target to control luciferase activity was determined in the presence of siRNA duplex and was normalized to the ratio observed in the absence of dsRNA. For comparison, the interference ratios of long dsRNAs (39 to 504 pb) are shown in FIG. 11B. The interference ratios were determined at concentrations of 5 nM for long dsRNAs (FIG.
  • FIG. 13A Two series of siRNA duplexes were prepared including the 21-nt siRNA duplex of FIG. 11H as reference. The length of the duplexes was varied between 20 to 25 bp by extending the base-paired segment at the 3′ end of the sense siRNA (FIG. 13B) or at the 3′ end of the antisense siRNA (FIG. 13C). Duplexes of 20 to 23 bp caused specific repression of target luciferase activity, but the 21-nt siRNA duplex was at least 8-fold more efficient than any of the other duplexes. 24- and 25-nt siRNA duplexes did not result in any detectable interference. Sequence-specific effects were minor as variations on both ends of the duplex produced similar effects.
  • siRNA ribose residues were examined (FIG. 14).
  • Substitution of the 2-nt 3′ overhangs by 2′-deoxy nucleotides had no effect, and even the replacement of two additional riboncleotides adjacent to the overhangs in the paired region, produced significantly active siRNAs.
  • 8 out of 42 nt of a siRNA duplex were replaced by DNA residues without loss of activity.
  • Complete substitution of one or both siRNA strands by 2′-deoxy residues abolished RNAi, as did substitution by 2′-O-methyl residues.
  • Target RNA cleavage positions were previously determined for 22-nt siRNA duplexes and for a 21-nt/22-nt duplex. It was found that the position of the target RNA cleavage was located in the centre of the region covered by the siRNA duplex, 11 or 12 nt downstream of the first nucleotide that was complementary to the 21- or 22-nt siRNA guide sequence.
  • Five distinct 21-nt siRNA duplexes with 2-nt 3′ overhang (FIG. 1 5 A) were incubated with 5′ cap-labelled sense or antisense target RNA in D. melanogaster lysate (Tuschl et al., 1999; Zamore et al., 2000).
  • the 5′ cleavage products were resolved on sequencing gels (FIG. 15B).
  • the amount of sense target RNA cleaved correlates with the efficiency of siRNA duplexes determined in the translation-based assay, and siRNA duplexes 1, 2 and 4 (FIGS. 15B and 11H, G, E) cleave target RNA faster than duplexes 3 and 5 (FIGS. 15B and 11F, D).
  • the sum of radioactivity of the 5′ cleavage product and the input target RNA were not constant over time, and the 5′ cleavage products did not accumulate.
  • the cleavage products, once released from the siRNA-endonuclease complex are rapidly degraded due to the lack of either of the poly(A) tail of the 5′-cap.
  • the cleavage sites for both, sense and antisense target RNAs were located in the middle of the region spanned by the siRNA duplexes.
  • the cleavage sites for each target produced by the 5 different duplexes varied by 1-nt according to the 1-nt displacement of the duplexes along the target sequences.
  • the targets were cleaved precisely 11 nt downstream of the target position complementary to the 3′-most nucleotide of the sequence-complementary guide siRNA (FIG. 15A, B).
  • a 2-nt 3′ overhang is preferred for siRNA function.
  • RISC or siRNP endonuclease complex
  • sequence changes were introduced by inverting short segments of 3- or 4-nt length or as point mutations (FIG. 18).
  • the sequence changes in one siRNA strand were compensated in the complementary siRNA strand to avoid pertubing the base-paired siRNA duplex structure.
  • the sequence of all 2-nt 3′ overhangs was TT (T, 2′-deoxythymidine) to reduce costs of synthesis.
  • the TT/TT reference siRNA duplex was comparable in RNAi to the wild-type siRNA duplex AA/UG (FIG. 17).
  • Efficiently silencing siRNA duplexes are preferably composed of 21-nt antisense siRNAs, and should be selected to form a 19 bp double helix with 2-nt 3′ overhanging ends.
  • 2′-deoxy substitutions of the 2-nt 3′ overhanging ribonucleotides do not affect RNAi, but help to reduce the costs of RNA synthesis and may enhance RNAse resistance of siRNA duplexes. More extensive 2′-deoxy or 2′-O-methyl modifications, however, reduce the ability of siRNAs to mediate RNAi, probably by interfering with protein association for siRNAP assembly.
  • Target recognition is a highly sequence-specific process, mediated by the siRNA complementary to the target.
  • the 3′-most nucleotide of the guide siRNA does not contribute to specificity of target recognition, while the penultimate nucleotide of the 3′ overhang affects target RNA cleavage, and a mismatch reduces RNAi 2- to 4-fold.
  • the 5′ end of a guide siRNA also appears more permissive for mismatched target RNA recognition when compared to the 3′ end.
  • Nucleotides in the centre of the siRNA, located opposite the target RNA cleavage site are important specificity determinants and even single nucleotide changes reduce RNAi to undetectable level. This suggests that siRNA duplexes may be able to discriminate mutant or polymorphic alleles in gene targeting experiments, which may become an important feature for future therapeutic developments.
  • siRNA sequences with identical 3′ overhanging sequences.
  • Asymmetry in reconstitution of sense and antisense-cleaving siRNPs could be (partially) responsible for the variation in RNAi efficiency observed for various 21-nt siRNA duplexes with 2-nt 3′ overhangs used in this study (FIG. 14).
  • the nucleotide sequence at the target site and/or the accessibility of the target RNA structure may be responsible for the variation in efficiency for these siRNA duplexes.
  • PKR a protein kinase regulated by double-stranded RNA. Int. J. Biochem. Cell Biol. 29, 945-949.
  • EGO-1 is related to RNA-directed RNA polymerase and functions in germ-line development and RNA interference in C. elegans. Curr. Biol. 10, 169-178.
  • RNAi Double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals. Cell 101, 25-33.
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Cited By (467)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020086356A1 (en) * 2000-03-30 2002-07-04 Whitehead Institute For Biomedical Research RNA sequence-specific mediators of RNA interference
US20030139363A1 (en) * 2001-07-23 2003-07-24 Kay Mark A. Methods and compositions for RNAi mediated inhibition of viral gene expression in mammals
US20030139585A1 (en) * 2001-07-12 2003-07-24 Eugen Uhlmann Synthetic double-stranded oligonucleotides for specific inhibition of gene expression
US20030153519A1 (en) * 2001-07-23 2003-08-14 Kay Mark A. Methods and compositions for RNAi mediated inhibition of gene expression in mammals
US20030157030A1 (en) * 2001-11-02 2003-08-21 Insert Therapeutics, Inc. Methods and compositions for therapeutic use of rna interference
US20040005593A1 (en) * 2002-03-06 2004-01-08 Rigel Pharmaceuticals, Inc. Novel method for delivery and intracellular synthesis of siRNA molecules
US20040014956A1 (en) * 2002-02-01 2004-01-22 Sequitur, Inc. Double-stranded oligonucleotides
US20040053411A1 (en) * 2002-05-03 2004-03-18 Duke University Method of regulating gene expression
US20040063654A1 (en) * 2001-11-02 2004-04-01 Davis Mark E. Methods and compositions for therapeutic use of RNA interference
US20040086845A1 (en) * 1999-10-20 2004-05-06 Tzyy-Choou Wu Superior molecular vaccine linking the translocation domain of a bacterial toxin to an antigen
US20040091457A1 (en) * 2001-10-12 2004-05-13 Ribopharma Ag Compositions and methods for inhibiting viral replication
US20040096843A1 (en) * 2002-02-14 2004-05-20 Rossi John J. Methods for producing interfering RNA molecules in mammalian cells and therapeutic uses for such molecules
US20040096882A1 (en) * 2002-08-21 2004-05-20 Martin Gleave RNAi probes targeting cancer-related proteins
US20040147027A1 (en) * 2003-01-28 2004-07-29 Troy Carol M. Complex for facilitating delivery of dsRNA into a cell and uses thereof
US20040180357A1 (en) * 2002-11-01 2004-09-16 The Trustees Of The University Of Pennsylvania Compositions and methods for siRNA inhibition of HIF-1 alpha
US20040192626A1 (en) * 2002-02-20 2004-09-30 Mcswiggen James RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US20040191905A1 (en) * 2002-11-22 2004-09-30 University Of Massachusetts Modulation of HIV replication by RNA interference
US20040203145A1 (en) * 2002-08-07 2004-10-14 University Of Massachusetts Compositions for RNA interference and methods of use thereof
US20040219671A1 (en) * 2002-02-20 2004-11-04 Sirna Therapeutics, Inc. RNA interference mediated treatment of parkinson disease using short interfering nucleic acid (siNA)
US20040221337A1 (en) * 1999-10-27 2004-11-04 Baulcombe David C. Gene silencing
US20040242518A1 (en) * 2002-09-28 2004-12-02 Massachusetts Institute Of Technology Influenza therapeutic
US20040248174A1 (en) * 2003-04-18 2004-12-09 Thetrustees Of The University Of Pennsylvania Compositions and methods for siRNA inhibition of angiopoietin 1and 2 and their receptor Tie2
US20040248296A1 (en) * 2002-03-20 2004-12-09 Beresford Paul J. HIV therapeutic
US20050004064A1 (en) * 2001-11-21 2005-01-06 Mitsubishi Chemical Corporation Method of inhibiting gene expression
US20050009042A1 (en) * 2003-02-10 2005-01-13 Aventis Pharma S.A. Oligonucleotides which inhibit expression of the OB-RGRP protein and method for detecting compounds which modify the interaction between proteins of the OB-RGRP family and the leptin receptor
US20050019918A1 (en) * 2003-06-03 2005-01-27 Hidetoshi Sumimoto Treatment of cancer by inhibiting BRAF expression
US20050037988A1 (en) * 2003-06-02 2005-02-17 University Of Massachusetts Methods and compositions for controlling efficacy of RNA silencing
US20050043524A1 (en) * 2003-08-18 2005-02-24 Isis Pharmaceuticals, Inc. Modulation of diacylglycerol acyltransferase 2 expression
US20050096289A1 (en) * 2002-02-07 2005-05-05 Hans Prydz Methods and compositions for modulating tissue factor
US20050136430A1 (en) * 2003-07-15 2005-06-23 California Institute Of Technology Inhibitor nucleic acids
US20050136437A1 (en) * 2003-08-25 2005-06-23 Nastech Pharmaceutical Company Inc. Nanoparticles for delivery of nucleic acids and stable double-stranded RNA
US20050142578A1 (en) * 2002-02-20 2005-06-30 Sirn Therapeutics, Inc. RNA interference mediated target discovery and target validation using short interfering nucleic acid (siNA)
US20050148526A1 (en) * 2002-01-23 2005-07-07 Kisielow Malgorzata A. Methods of obtaining isoform specific expression in mammalian cells
US20050159381A1 (en) * 2001-05-18 2005-07-21 Sirna Therapeutics, Inc. RNA interference mediated inhibition of chromosome translocation gene expression using short interfering nucleic acid (siNA)
US20050164212A1 (en) * 2003-03-06 2005-07-28 Todd Hauser Modulation of gene expression using DNA-RNA hybrids
US20050176024A1 (en) * 2001-05-18 2005-08-11 Sirna Therapeutics, Inc. RNA interference mediated inhibition of epidermal growth factor receptor (EGFR) gene expression using short interfering nucleic acid (siNA)
US20050176025A1 (en) * 2001-05-18 2005-08-11 Sirna Therapeutics, Inc. RNA interference mediated inhibition of B-cell CLL/Lymphoma-2 (BCL-2) gene expression using short interfering nucleic acid (siNA)
US20050181382A1 (en) * 2003-06-02 2005-08-18 University Of Massachusetts Methods and compositions for enhancing the efficacy and specificity of RNAi
US20050202075A1 (en) * 2004-03-12 2005-09-15 Pardridge William M. Delivery of genes encoding short hairpin RNA using receptor-specific nanocontainers
US20050208658A1 (en) * 2003-11-21 2005-09-22 The University Of Maryland RNA interference mediated inhibition of 11beta hydroxysteriod dehydrogenase-1 (11beta HSD-1) gene expression
US20050227256A1 (en) * 2003-11-26 2005-10-13 Gyorgy Hutvagner Sequence-specific inhibition of small RNA function
US20050234000A1 (en) * 2003-12-12 2005-10-20 Mitchell Gordon S SiRNA delivery into mammalian nerve cells
US20050239731A1 (en) * 2001-05-18 2005-10-27 Sirna Therapeutics, Inc. RNA interference mediated inhibition of MAP kinase gene expression using short interfering nucleic acid (siNA)
US20050246794A1 (en) * 2002-11-14 2005-11-03 Dharmacon Inc. Functional and hyperfunctional siRNA
US20050256076A1 (en) * 2004-03-26 2005-11-17 Curis, Inc. RNA interference modulators of hedgehog signaling and uses thereof
US20050256071A1 (en) * 2003-07-15 2005-11-17 California Institute Of Technology Inhibitor nucleic acids
US20050260214A1 (en) * 2004-05-12 2005-11-24 Simon Michael R Composition and method for introduction of RNA interference sequences into targeted cells and tissues
US20050272682A1 (en) * 2004-03-22 2005-12-08 Evers Bernard M SiRNA targeting PI3K signal transduction pathway and siRNA-based therapy
US20050273868A1 (en) * 2004-02-17 2005-12-08 University Of Massachusetts Methods and compositions for enhancing RISC activity in vitro and in vivo
US20050277610A1 (en) * 2004-03-15 2005-12-15 City Of Hope Methods and compositions for the specific inhibition of gene expression by double-stranded RNA
US20060003915A1 (en) * 2002-04-18 2006-01-05 Karina Drumm Means and methods for the specific modulation of target genes in the cns and the eye and methods for their identification
US20060008907A1 (en) * 2004-06-09 2006-01-12 The Curators Of The University Of Missouri Control of gene expression via light activated RNA interference
US20060009409A1 (en) * 2002-02-01 2006-01-12 Woolf Tod M Double-stranded oligonucleotides
US20060008910A1 (en) * 2004-06-07 2006-01-12 Protiva Biotherapeuties, Inc. Lipid encapsulated interfering RNA
US20060014289A1 (en) * 2004-04-20 2006-01-19 Nastech Pharmaceutical Company Inc. Methods and compositions for enhancing delivery of double-stranded RNA or a double-stranded hybrid nucleic acid to regulate gene expression in mammalian cells
US20060025366A1 (en) * 2004-07-02 2006-02-02 Protiva Biotherapeutics, Inc. Immunostimulatory siRNA molecules and uses therefor
US20060030003A1 (en) * 2004-05-12 2006-02-09 Simon Michael R Composition and method for introduction of RNA interference sequences into targeted cells and tissues
WO2006020557A2 (en) * 2004-08-10 2006-02-23 Immusol, Inc. Methods of using or identifying agents that inhibit cancer growth
WO2005044976A3 (en) * 2003-06-20 2006-02-23 Isis Pharmaceuticals Inc Oligomeric compounds for use in gene modulation
US20060040882A1 (en) * 2004-05-04 2006-02-23 Lishan Chen Compostions and methods for enhancing delivery of nucleic acids into cells and for modifying expression of target genes in cells
US20060051815A1 (en) * 2004-06-25 2006-03-09 The J. David Gladstone Institutes Methods of treating smooth muscle cell disorders
US20060058252A1 (en) * 2002-06-26 2006-03-16 Clawson Gary A Methods and materials for treating human papillomavirus infections
US20060063181A1 (en) * 2004-08-13 2006-03-23 Green Pamela J Method for identification and quantification of short or small RNA molecules
WO2006033965A2 (en) * 2004-09-16 2006-03-30 The Trustees Of The University Of Pennsylvania Nadph oxidase inhibition pharmacotherapies for obstructive sleep apnea syndrome and its associated morbidities
US20060069050A1 (en) * 2004-02-17 2006-03-30 University Of Massachusetts Methods and compositions for mediating gene silencing
US20060078902A1 (en) * 2004-04-15 2006-04-13 Michaeline Bunting Method and compositions for RNA interference
US20060083780A1 (en) * 2004-06-07 2006-04-20 Protiva Biotherapeutics, Inc. Cationic lipids and methods of use
US20060089323A1 (en) * 2004-10-22 2006-04-27 Sailen Barik RNAi modulation of RSV, PIV and other respiratory viruses and uses thereof
US20060094676A1 (en) * 2004-10-29 2006-05-04 Ronit Lahav Compositions and methods for treating cancer using compositions comprising an inhibitor of endothelin receptor activity
US20060128650A1 (en) * 2002-11-04 2006-06-15 University Of Massachusetts Allele-specific RNA interference
US20060134787A1 (en) * 2004-12-22 2006-06-22 University Of Massachusetts Methods and compositions for enhancing the efficacy and specificity of single and double blunt-ended siRNA
US20060134189A1 (en) * 2004-11-17 2006-06-22 Protiva Biotherapeutics, Inc siRNA silencing of apolipoprotein B
US20060166921A1 (en) * 2005-01-07 2006-07-27 Rachel Meyers RNAi modulation of RSV and therapeutic uses thereof
US20060172965A1 (en) * 2005-02-01 2006-08-03 Alcon, Inc. RNAi-mediated inhibition of ocular targets
US20060178328A1 (en) * 2002-11-26 2006-08-10 Medtronic Inc. Devices, systems and methods for improving memory and/or cognitive function through brain delivery of siRNA
US20060178297A1 (en) * 2003-01-28 2006-08-10 Troy Carol M Systems and methods for silencing expression of a gene in a cell and uses thereof
US20060189564A1 (en) * 2004-10-22 2006-08-24 Medtronic, Inc. Methods and sequences to suppress pro-inflamatory cytokine actions locally to treat pain
US20060205635A1 (en) * 2005-03-14 2006-09-14 Board Of Regents, The University Of Texas System Antigene oligomers inhibit transcription
US20060211637A1 (en) * 2002-08-06 2006-09-21 Intradigm Corporation Methods of down regulating target gene expression in vivo by introduction of interfering rna
US20060217324A1 (en) * 2005-01-24 2006-09-28 Juergen Soutschek RNAi modulation of the Nogo-L or Nogo-R gene and uses thereof
US20060223773A1 (en) * 2005-03-11 2006-10-05 Alcon, Inc. RNAi-mediated inhibition of Frizzled Related Protein-1 for treatment of glaucoma
US20060223749A1 (en) * 2001-09-19 2006-10-05 University Of South Florida Inhibition of SHIP to enhance stem cell harvest and transplantation
US20060240425A1 (en) * 2002-09-30 2006-10-26 Oncotherapy Science, Inc Genes and polypeptides relating to myeloid leukemia
US20060240093A1 (en) * 2003-07-16 2006-10-26 Protiva Biotherapeutics, Inc. Lipid encapsulated interfering rna
US20060239971A1 (en) * 2003-02-21 2006-10-26 Mohapatra Shyam S Vectors for regulating gene expression
US20060257380A1 (en) * 2002-09-19 2006-11-16 Inst.Nat. De La Sante Et De La Recherche MED Use of sirnas for gene silencing in antigen presenting cells
US20060269530A1 (en) * 2003-02-21 2006-11-30 The Penn State Research Foundation RNA interference compositions and methods
US20060269519A1 (en) * 2004-07-19 2006-11-30 Baylor College Of Medicine Modulation of cytokine signaling regulators and applications for immunotherapy
US20060276635A1 (en) * 2002-09-05 2006-12-07 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US20060287269A1 (en) * 2002-09-09 2006-12-21 The Regents Of The University Of California Short interfering nucleic acid hybrids and methods thereof
US20060287259A1 (en) * 2003-12-23 2006-12-21 The Trustees Of The University Of Pennsylvania Compositions and methods for combined therapy of disease
US20060293256A1 (en) * 2002-08-06 2006-12-28 Masateru Yamada Remedy or preventive for kidney disease and method of diagnosing kidney disease
US20060292119A1 (en) * 2005-06-23 2006-12-28 Baylor College Of Medicine Modulation of negative immune regulators and applications for immunotherapy
US20070003575A1 (en) * 2004-05-26 2007-01-04 Itzhak Bentwich Viral and viral associated MiRNAs and uses thereof
US20070010468A1 (en) * 2003-04-23 2007-01-11 Georgetown University Methods and compositions for the inhibition of stat5 in prostate cancer cells
US20070037762A1 (en) * 2002-07-24 2007-02-15 Tolentino Michael J COMPOSITIONS AND METHODS FOR siRNA INHIBITION OF ANGIOGENESIS
US20070054873A1 (en) * 2005-08-26 2007-03-08 Protiva Biotherapeutics, Inc. Glucocorticoid modulation of nucleic acid-mediated immune stimulation
US20070087991A1 (en) * 2002-02-13 2007-04-19 Axordia Limited Pluripotential stem cells
US20070111227A1 (en) * 2005-07-28 2007-05-17 Green Pamela J Small regulatory RNAs and methods of use
US20070111963A1 (en) * 2005-11-17 2007-05-17 Board Of Regents, The University Of Texas System Modulation of gene expression by oligomers targeted to chromosomal DNA
US20070128640A1 (en) * 2002-11-14 2007-06-07 Dharmacon, Inc. siRNA targeting ras-related nuclear protein
US20070135372A1 (en) * 2005-11-02 2007-06-14 Protiva Biotherapeutics, Inc. Modified siRNA molecules and uses thereof
US20070135370A1 (en) * 2005-10-20 2007-06-14 Protiva Biotherapeutics, Inc. siRNA silencing of filovirus gene expression
US20070141601A1 (en) * 2004-05-12 2007-06-21 Dharmacon, Inc. siRNA targeting cAMP-specific phosphodiesterase 4D
US20070141009A1 (en) * 2003-01-03 2007-06-21 Shaharyar Khan Sirna mediated post-transriptional gene silencing of genes involved in alopecia
US20070149470A1 (en) * 2004-09-10 2007-06-28 Kaspar Roger L Inhibition of viral gene expression using small interfering RNA
US20070149468A1 (en) * 2003-05-16 2007-06-28 Jackson Aimee L Methods and compositions for rna interference
US20070160586A1 (en) * 2005-06-15 2007-07-12 Children's Medical Center Corporation Methods for extending the replicative lifespan of cells
US20070161586A1 (en) * 2004-01-16 2007-07-12 Takeda Pharmaceutical Company Limited Drug for preventing and treating atherosclerosis
US20070161547A1 (en) * 2003-06-03 2007-07-12 Balkrishen Bhat Modulation of survivin expression
US20070180242A1 (en) * 2006-01-30 2007-08-02 Nagaraj Thadi M GSM authentication in a CDMA network
US20070202134A1 (en) * 2004-02-23 2007-08-30 Kufe Donald W Muc1 Antagonist Enhancement of Death Receptor Ligand-Induced Apoptosis
US20070232555A1 (en) * 2004-03-26 2007-10-04 Nariyoshi Shinomiya C-Met Sirna Adenovirus Vectors Inhibit Cancer Cell Growth, Invasion and Tumorigenicity
US20070238676A1 (en) * 2003-12-04 2007-10-11 Mohapatra Shyam S Polynucleotides for Reducing Respiratory Syncytial Virus Gene Expression
US20070238691A1 (en) * 2006-03-29 2007-10-11 Senesco Technologies, Inc. Inhibition of HIV replication and expression of p24 with eIF-5A
US20070244311A1 (en) * 2002-11-14 2007-10-18 Dharmacon, Inc. siRNA targeting coatomer protein complex, subunit beta 2 (CPOB2)
US20070243570A1 (en) * 2003-05-19 2007-10-18 Genecare Research Institute Co., Ltd Apoptosis Inducer for Cancer Cell
US20070258993A1 (en) * 2003-11-12 2007-11-08 The Austin Research Institute Dna-Carrier Conjugate
US20070259827A1 (en) * 2006-01-25 2007-11-08 University Of Massachusetts Compositions and methods for enhancing discriminatory RNA interference
US20070265220A1 (en) * 2004-03-15 2007-11-15 City Of Hope Methods and compositions for the specific inhibition of gene expression by double-stranded RNA
US20070269892A1 (en) * 2006-05-18 2007-11-22 Nastech Pharmaceutical Company Inc. FORMULATIONS FOR INTRACELLULAR DELIVERY dsRNA
US20070270369A1 (en) * 2002-09-18 2007-11-22 The Burnham Institute Use of hepatitis b x-interacting protein (hbxip) in modulation of apoptosis
US20070275923A1 (en) * 2006-05-25 2007-11-29 Nastech Pharmaceutical Company Inc. CATIONIC PEPTIDES FOR siRNA INTRACELLULAR DELIVERY
US20070275913A1 (en) * 2006-04-12 2007-11-29 Monia Brett P Compositions and their uses directed to hepcidin
US20080014191A1 (en) * 2006-05-19 2008-01-17 The Scripps Research Institute Treatment of Protein Misfolding
US20080039411A1 (en) * 2004-02-23 2008-02-14 Ulf Smith Use Of Resistin Antisense Oligonucleotides And/Or Sirna Molecules In The Treatment Of Rheumatoid Arthritis
US20080039412A1 (en) * 2004-02-10 2008-02-14 Sirna Therapeutics, Inc. Rna Interference Mediated Inhibition of Gene Expression Using Multifunctional Short Interfering Nucleic Acid (Multifunctional Sina)
US20080039415A1 (en) * 2006-08-11 2008-02-14 Gregory Robert Stewart Retrograde transport of sirna and therapeutic uses to treat neurologic disorders
US20080057062A1 (en) * 2006-07-18 2008-03-06 National Institute Of Advanced Industrial Science And Technology Agent for Inducing senescence and apoptosis of cancer cell
US20080069840A1 (en) * 2005-01-06 2008-03-20 Tzyy-Choou Wu RNA Interference That Blocks Expression of Pro-Apoptotic Proteins Potentiates Immunity Induced by DNA and Transfected Dendritic Cell Vaccines
US20080070857A1 (en) * 2005-02-14 2008-03-20 Jun Nishihira Pharmaceutical Agents for Preventing Metastasis of Cancer
US20080076731A1 (en) * 2000-09-19 2008-03-27 University Of South Florida Control of NK cell function and survival by modulation of ship activity
US20080102084A1 (en) * 2005-01-26 2008-05-01 Tzyy-Choou Wu Anti-cancer DNA Vaccine Employing Plasmids Encoding Mutant Oncoprotein Antigen and Calreticulin
US20080108584A1 (en) * 2006-05-22 2008-05-08 De Fougerolles Antonin Compositions and methods for inhibiting expression of ikk-b gene
US20080125384A1 (en) * 2005-11-21 2008-05-29 Shuewi Yang Simultaneous silencing and restoration of gene function
US20080152654A1 (en) * 2006-06-12 2008-06-26 Exegenics, Inc., D/B/A Opko Health, Inc. COMPOSITIONS AND METHODS FOR siRNA INHIBITION OF ANGIOGENESIS
US20080153765A1 (en) * 2004-03-22 2008-06-26 Gewirtz Alan M Methods of Use of Bcl-6-Derived Nucleotides to Induce Apoptosis
US20080167256A1 (en) * 2004-03-31 2008-07-10 Hidetoshi Sumimoto Cancer Therapy Via the Inhibition of Skp-2 Expression
US20080167265A1 (en) * 2007-01-09 2008-07-10 Isis Pharmaceuticals Inc Modulation of fr-alpha expression
US20080171051A1 (en) * 2003-11-26 2008-07-17 Patrick Gerard Johnston Cancer Treatment
US20080171719A1 (en) * 2006-11-28 2008-07-17 Alcon Manufacturing, Ltd. RNAi-MEDIATED INHIBITION OF AQUAPORIN 1 FOR TREATMENT OF IOP-RELATED CONDITIONS
US20080171715A1 (en) * 2004-11-12 2008-07-17 David Brown Methods and compositions involving mirna and mirna inhibitor molecules
US20080171906A1 (en) * 2007-01-16 2008-07-17 Everaerts Frank J L Tissue performance via hydrolysis and cross-linking
US20080177051A1 (en) * 2002-11-14 2008-07-24 Dharmacon, Inc. siRNA targeting cyclin-dependent kinase inhibitor 1B (p27, Kip1) (CDKN1B)
US20080176293A1 (en) * 2005-07-25 2008-07-24 Jacques Rohayem RNA-Dependent RNA Polymerase, Methods And Kits For The Amplification And/Or Labelling Of RNA
US7422853B1 (en) * 2002-10-04 2008-09-09 Myriad Genetics, Inc. RNA interference using a universal target
US20080221059A1 (en) * 2008-04-03 2008-09-11 National Taiwan University Novel treatment tool for cancer: rna interference of bcas2
US20080227967A1 (en) * 2002-11-14 2008-09-18 Dharmacon, Inc. siRNA targeting ribonucleotide reductase M2 polypeptide (RRM2 or RNR-R2)
US20080242627A1 (en) * 2000-08-02 2008-10-02 University Of Southern California Novel rna interference methods using dna-rna duplex constructs
US20080249038A1 (en) * 2003-10-07 2008-10-09 Quark Biotech, Inc. Bone Morphogenetic Protein (Bmp) 2A and Uses Thereof
US20080249046A1 (en) * 2006-06-09 2008-10-09 Protiva Biotherapeutics, Inc. MODIFIED siRNA MOLECULES AND USES THEREOF
WO2008002678A3 (en) * 2006-06-29 2008-10-16 Univ Texas Structural-based inhibitors of the glutathione binding site in aldose reductase, methods of screening therefor and methods of use
WO2008088836A3 (en) * 2007-01-16 2008-10-16 Burnham Inst Medical Research Compositions and methods for treatment of colorectal cancer
US20080255345A1 (en) * 2006-11-21 2008-10-16 Alnylam Pharmaceuticals, Inc. IRNA Agents Targeting CCR5 Expressing Cells And Uses Thereof
US20080253989A1 (en) * 2004-10-22 2008-10-16 Neuregenix Limited Neuron Regeneration
US20080260765A1 (en) * 2007-03-15 2008-10-23 Johns Hopkins University HPV DNA Vaccines and Methods of Use Thereof
WO2007127919A3 (en) * 2006-04-28 2008-10-30 Alnylam Pharmaceuticals Inc Compositions and methods for inhibiting expression of a gene from the jc virus
US20080268457A1 (en) * 2002-11-14 2008-10-30 Dharmacon, Inc. siRNA targeting forkhead box P3 (FOXP3)
US20080279844A1 (en) * 2006-10-18 2008-11-13 Kapil Mehta Methods for Treating Cancer Targeting Transglutaminase
US20080287386A1 (en) * 2004-01-30 2008-11-20 Quark Biotech, Inc. Oligoribonucleotides and methods of use thereof for treatment of fibrotic conditions and other diseases
US20080299659A1 (en) * 2007-03-02 2008-12-04 Nastech Pharmaceutical Company Inc. Nucleic acid compounds for inhibiting apob gene expression and uses thereof
US20080319180A1 (en) * 2002-11-14 2008-12-25 Dharmacon, Inc. siRNA targeting protein kinase N-3 (PKN-3)
US20090005330A1 (en) * 2004-08-31 2009-01-01 Sylentis S.A. Methods and Compositions to Inhibit P2x7 Receptor Expression
US20090005548A1 (en) * 2002-11-14 2009-01-01 Dharmacon, Inc. siRNA targeting nuclear receptor interacting protein 1 (NRIP1)
US20090023675A1 (en) * 2002-02-20 2009-01-22 Sirna Therapeutics, Inc. RNA Interference Mediated Inhibition of Gene Expression Using Chemically Modified Short Interfering Nucleic Acid (siNA)
US20090022667A1 (en) * 2007-05-15 2009-01-22 Marco Peters METHODS OF TREATING COGNITIVE DISORDERS BY INHIBITION OF Gpr12
US20090028862A1 (en) * 2004-09-30 2009-01-29 Arndt Gregory M Emmprin antagonists and uses thereof
US20090042826A1 (en) * 2002-02-12 2009-02-12 Quark Pharmaceuticals, Inc. Use of the AXL receptor for diagnosis and treatment of renal disease
US20090053298A1 (en) * 2007-07-31 2009-02-26 Sumitomo Chemical Company, Limited Compositions and methods for inhibiting car gene expression by rna interference
US20090061487A1 (en) * 2006-09-08 2009-03-05 Samuel Jotham Reich Sirna and methods of manufacture
US20090081789A1 (en) * 2007-08-31 2009-03-26 Greenville Hospital System Activation of nuclear factor kappa B
US20090093435A1 (en) * 2002-02-20 2009-04-09 Sirna Therapeutics, Inc. RNA INTERFERENCE MEDIATED INHIBITION OF GRB2 ASSOCIATED BINDING PROTEIN (GAB2) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
US20090092988A1 (en) * 2007-10-04 2009-04-09 Schwartz Jacob C Modulating Gene Expression with agRNA and Gapmers Targeting Antisense Transcripts
US20090093437A1 (en) * 2002-02-20 2009-04-09 Sirna Therapeutics, Inc. RNA INTERFERENCE MEDIATED INHIBITION OF CHECKPOINT KINASE-1 (CHK-1) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
US20090093439A1 (en) * 2002-02-20 2009-04-09 Sirna Therapeutics, Inc. RNA INTERFERENCE MEDIATED INHIBITION OF CHROMOSOME TRANSLOCATION GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
US20090099112A1 (en) * 2004-08-23 2009-04-16 Sylentis S.A.U. Methods and Compositions for the Treatment of Eye Disorders with Increased Intraocular Pressure
US20090099116A1 (en) * 2002-02-20 2009-04-16 Sirna Therapeutics, Inc. RNA INTERFERENCE MEDIATED INHIBITION OF FOS GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
US20090098127A1 (en) * 2004-07-12 2009-04-16 Medical Research Fund Of Tel Aviv Sourasky Medical Center Agents Capable of Downregulating an MSF-A DEPENDENT HIF-1ALPHA and Use Thereof in Cancer Treatment
US20090118206A1 (en) * 2003-09-12 2009-05-07 University Of Massachusetts Rna interference for the treatment of gain-of-function disorders
US20090118489A1 (en) * 2002-11-14 2009-05-07 Dharmacon, Inc. siRNA targeting nucleoporin 62kDa (Nup62)
US20090118214A1 (en) * 2007-11-07 2009-05-07 Beeologics, Llc Compositions for conferring tolerance to viral disease in social insects, and the use thereof
US20090123572A1 (en) * 2005-03-18 2009-05-14 Shiseido Company, Ltd. Method and Pharmaceutical Composition for Treating Psoriasis, Squamous Cell Carcinoma and/or Parakeratosis by Inhibiting Expression of Squamous Cell Carcinoma-Related Antigen
US20090130212A1 (en) * 2006-05-15 2009-05-21 Physical Pharmaceutica, Llc Composition and improved method for preparation of small particles
US20090137509A1 (en) * 2002-02-20 2009-05-28 Sirna Therapeutics, Inc. RNA INTERFERENCE MEDIATED INHIBITION OF PROLIFERATION CELL NUCLEAR ANTIGEN (PCNA) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
US20090137508A1 (en) * 2002-02-20 2009-05-28 Sirna Therapeutics, Inc. RNA INTERFERENCE MEDIATED INHIBITION OF POLYCOMB GROUP PROTEIN EZH2 GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
US20090143321A1 (en) * 2005-07-07 2009-06-04 Avraham Hochberg Nucleic acid agents for downregulating h19 and methods of using same
US20090148471A1 (en) * 2000-08-03 2009-06-11 The Johns Hopkins University Molecular Vaccine Linking an Endoplasmic Reticulum Chaperone Polypeptide to an Antigen
US20090149377A1 (en) * 2005-09-30 2009-06-11 St. Jude Children's Research Hospital METHODS FOR REGULATION OF p53 TRANSLATION AND FUNCTION
US20090149403A1 (en) * 2006-05-26 2009-06-11 Protiva Biotherapeutics, Inc. siRNA silencing of genes expressed in cancer
US20090149407A1 (en) * 2000-08-30 2009-06-11 Sirna Therapeutics, Inc. RNA INTERFERENCE MEDIATED TREATMENT OF ALZHEIMER'S DISEASE USING SHORT INTERFERING NUCLEIC ACID (siNA)
US20090156531A1 (en) * 2005-12-30 2009-06-18 Institut Gustave Roussy Use of Inhibitors of Scinderin and/or Ephrin-A1 for Treating Tumors
US20090163407A1 (en) * 2004-11-15 2009-06-25 Mount Sinai School Of Medicine Of New York University Compositions and methods for altering wnt autocrine signaling
US20090192113A1 (en) * 2003-08-28 2009-07-30 Jan Weiler Interfering RNA Duplex Having Blunt-Ends and 3`-Modifications
US20090203055A1 (en) * 2005-04-18 2009-08-13 Massachusetts Institute Of Technology Compositions and methods for RNA interference with sialidase expression and uses thereof
US20090203138A1 (en) * 2008-02-12 2009-08-13 University Of Tennessee Research Foundation, Inc. Small Interfering RNAs Targeting Feline Herpes Virus
US20090203135A1 (en) * 2007-04-23 2009-08-13 Alnylam Pharmaceuticals, Inc. Glycoconjugates of RNA Interference Agents
US7579451B2 (en) 2004-07-21 2009-08-25 Alnylam Pharmaceuticals, Inc. Oligonucleotides comprising a modified or non-natural nucleobase
US20090226446A1 (en) * 2006-04-06 2009-09-10 Deutsches Krebsforschungszentrum Stiftung Des Offentilchen Rechts Method to Inhibit the Propagation of an Undesired Cell Population
US20090227780A1 (en) * 2002-11-14 2009-09-10 Dharmacon, Inc. siRNA targeting connexin 43
US20090233983A1 (en) * 2002-02-20 2009-09-17 Sirna Therapeutics Inc. RNA Interference Mediated Inhibition of Protein Tyrosine Phosphatase-1B (PTP-1B) Gene Expression Using Short Interfering RNA
US20090238772A1 (en) * 2007-12-13 2009-09-24 Alnylam Pharmaceuticals, Inc. Methods and compositions for prevention or treatment of rsv infection
US20090239814A1 (en) * 2007-12-04 2009-09-24 Alnylam Pharmaceuticals, Inc. Carbohydrate Conjugates as Delivery Agents for Oligonucleotides
US20090247613A1 (en) * 2002-02-20 2009-10-01 Sirna Therapeutics, Inc. RNA INTERFERENCE MEDIATED INHIBITION OF B-CELL CLL/LYMPHOMA-2 (BCL2) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
US20090247606A1 (en) * 2001-08-28 2009-10-01 Sirna Therapeutics, Inc. RNA Interference Mediated Inhibition of Adenosine A1 Receptor (ADORA1) Gene Expression Using Short Interfering Nucleic Acid (siNA)
US20090247607A1 (en) * 2006-03-24 2009-10-01 John Benson dsRNA COMPOSITIONS AND METHODS FOR TREATING HPV INFECTION
US20090247614A1 (en) * 2007-12-04 2009-10-01 Alnylam Pharmaceuticals, Inc. Folate Conjugates
US20090253772A1 (en) * 2002-02-20 2009-10-08 Sirna Therapeutics, Inc. RNA INTERFERENCE MEDIATED INHIBITION OF CXCR4 GENE EXPRESSION USING SHORT INTERFERING NUCELEIC ACID (siNA)
US20090258934A1 (en) * 2005-11-04 2009-10-15 Alnylam Pharmaceuticals, Inc. COMPOSITIONS AND METHODS FOR INHIBITING EXPRESSION OF Nav1.8 GENE
US7605249B2 (en) 2002-11-26 2009-10-20 Medtronic, Inc. Treatment of neurodegenerative disease through intracranial delivery of siRNA
US20090275638A1 (en) * 2008-04-17 2009-11-05 Kevin Fitzgerald Compositions and Methods for Inhibiting Expression of XBP-1 Gene
US20090275635A1 (en) * 2006-05-26 2009-11-05 Medical Research Council Screening method
US7615618B2 (en) 2004-06-30 2009-11-10 Alnylam Pharmaceuticals, Inc. Oligonucleotides comprising a non-phosphate backbone linkage
US20090285861A1 (en) * 2008-04-17 2009-11-19 Tzyy-Choou Wu Tumor cell-based cancer immunotherapeutic compositions and methods
US7626014B2 (en) 2004-04-27 2009-12-01 Alnylam Pharmaceuticals Single-stranded and double-stranded oligonucleotides comprising a 2-arylpropyl moiety
US20090306182A1 (en) * 2002-02-20 2009-12-10 Sirna Therapeutics, Inc. RNA INTERFERENCE MEDIATED INHIBITION OF MAP KINASE GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
US20090306356A1 (en) * 2002-11-14 2009-12-10 Dharmacon,Inc. siRNA Targeting TNFalpha
US7632932B2 (en) 2004-08-04 2009-12-15 Alnylam Pharmaceuticals, Inc. Oligonucleotides comprising a ligand tethered to a modified or non-natural nucleobase
US20100003317A1 (en) * 2008-03-27 2010-01-07 Akin Akinc Compositions and methods for mediating rnai in vivo
US20100010066A1 (en) * 2008-01-31 2010-01-14 Kevin Fitzgerald Optimized Methods For Delivery Of DSRNA Targeting The PCSK9 Gene
US20100015707A1 (en) * 2006-05-04 2010-01-21 Francois Jean-Charles Natt SHORT INTERFERING RIBONUCLEIC ACID (siRNA) FOR ORAL ADMINISTRATION
US20100016405A1 (en) * 2006-07-10 2010-01-21 Alnylam Pharmaceuticals, Inc Compositions and Methods for Inhibiting Expression of the MYC Gene
US20100035966A1 (en) * 2006-06-14 2010-02-11 Rosetta Inpharmatics Llc Methods and compositions for regulating cell cycle progression
US7674778B2 (en) 2004-04-30 2010-03-09 Alnylam Pharmaceuticals Oligonucleotides comprising a conjugate group linked through a C5-modified pyrimidine
US20100069461A1 (en) * 2005-11-09 2010-03-18 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of factor v leiden mutant gene
US20100087508A1 (en) * 2008-03-05 2010-04-08 David Bumcrot Compositions and Methods for Inhibiting Expression of Eg5 and VEGF Genes
US7695902B2 (en) 1996-06-06 2010-04-13 Isis Pharmaceuticals, Inc. Oligoribonucleotides and ribonucleases for cleaving RNA
US20100093831A1 (en) * 2003-03-12 2010-04-15 Vasgene Therapeutics, Inc. Nucleic acid compounds for inhibiting angiogenesis and tumor growth
US20100098664A1 (en) * 2007-11-28 2010-04-22 Mathieu Jean-Francois Desclaux Lentiviral vectors allowing RNAi mediated inhibition of GFAP and vimentin expression
US20100105759A1 (en) * 2007-01-16 2010-04-29 Abraham Hochberg H19 silencing nucleic acid agents for treating rheumatoid arthritis
US7709616B2 (en) 2004-05-14 2010-05-04 Rosetta Genomics Inc. Micrornas and uses thereof
US20100113307A1 (en) * 2002-11-14 2010-05-06 Dharmacon, Inc. siRNA targeting vascular endothelial growth factor (VEGF)
US20100113564A1 (en) * 2002-02-20 2010-05-06 Mcswiggen James RNA Interference Mediated Inhibition of Interleukin and Interleukin Receptor Gene Expression Using Short Interfering Nucleic Acid (siNA)
US20100112686A1 (en) * 2008-10-15 2010-05-06 Qing Ge Short hairpin rnas for inhibition of gene expression
US20100120893A1 (en) * 2008-10-20 2010-05-13 Dinah Wen-Yee Sah Compositions and Methods for Inhibiting Expression of Transthyretin
US20100130595A1 (en) * 2008-08-25 2010-05-27 Dean Nicholas M Antisense oligonucleotides directed against connective tissue growth factor and uses thereof
US20100129429A1 (en) * 2005-11-07 2010-05-27 British Columbia Cancer Agency Inhibition of autophagy genes in cancer chemotherapy
US20100136026A1 (en) * 2007-09-26 2010-06-03 Kerr William G Ship Inhibition to Direct Hematopoietic Stem Cells and Induce Extramedullary Hematopoiesis
US7732591B2 (en) 2003-11-25 2010-06-08 Medtronic, Inc. Compositions, devices and methods for treatment of huntington's disease through intracranial delivery of sirna
US20100144851A1 (en) * 2002-02-20 2010-06-10 Sirna Therapeutics, Inc. RNA Interference Mediated Inhibition of Platelet-Derived Endothelial Cell Growth Factor (ECGF1) Gene Expression Using Short Interfering Nucleic Acid (siNA)
US20100144833A1 (en) * 2004-10-22 2010-06-10 Sailen Barik RNAi Modulation Of RSV, PIV And Other Respiratory Viruses And Uses Thereof
US20100144842A1 (en) * 2002-02-20 2010-06-10 Sirna Therapeutics, Inc. RNA Interference Mediated Inhibition of NOGO and NOGO Receptor Gene Expression Using Short Interfering Nucleic Acid (siNA)
US20100151470A1 (en) * 2007-05-01 2010-06-17 University Of Massachusetts Methods and compositions for locating snp heterozygosity for allele specific diagnosis and therapy
US7745418B2 (en) 2001-10-12 2010-06-29 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting viral replication
US20100166661A1 (en) * 2008-12-11 2010-07-01 Bojian Zheng siRNA COMPOSITIONS AND METHODS FOR POTENTLY INHIBITING VIRAL INFECTION
US20100168206A1 (en) * 2008-12-10 2010-07-01 Jared Gollob GNAQ Targeted dsRNA Compositions And Methods For Inhibiting Expression
US20100168205A1 (en) * 2008-10-23 2010-07-01 Alnylam Pharmaceuticals, Inc. Methods and Compositions for Prevention or Treatment of RSV Infection Using Modified Duplex RNA Molecules
US7750144B2 (en) 2003-06-02 2010-07-06 University Of Massachusetts Methods and compositions for enhancing the efficacy and specificity of RNA silencing
US20100184824A1 (en) * 2002-02-20 2010-07-22 Sirna Therapeutics, Inc. RNA Interference Mediated Inhibition of Interleukin and Interleukin Receptor Gene Expression Using Short Interfering Nucleic Acid (siNA)
US20100183696A1 (en) * 2007-01-30 2010-07-22 Allergan, Inc Treating Ocular Diseases Using Peroxisome Proliferator-Activated Receptor Delta Antagonists
US20100184825A1 (en) * 2002-02-20 2010-07-22 Merck Sharp & Dohme Corp. RNA Interference Mediated Inhibition of Protein Tyrosine Phosphatase-1B (PTP-1B) Gene Expression Using Short Interfering Nucleic Acid (siNA)
US7763592B1 (en) 2003-11-20 2010-07-27 University Of South Florida SHIP-deficiency to increase megakaryocyte progenitor production
US20100190714A1 (en) * 2007-06-15 2010-07-29 Novatis Ag RNAi Inhibition of Alpha-ENaC Expression
US20100196403A1 (en) * 2007-01-29 2010-08-05 Jacob Hochman Antibody conjugates for circumventing multi-drug resistance
US20100197773A1 (en) * 2009-02-03 2010-08-05 Birgit Bramlage Compositions and methods for inhibiting expression of ptp1b genes
US20100204307A1 (en) * 2006-09-21 2010-08-12 Tomoko Nakayama Compositions And Methods For Inhibiting Expression Of The HAMP Gene
US20100204306A1 (en) * 2007-12-14 2010-08-12 Alnylam Pharmaceuticals, Inc. Method of Treating Neurodegenerative Disease
US7781575B2 (en) 2002-11-14 2010-08-24 Dharmacon, Inc. siRNA targeting tumor protein 53 (p53)
US20100216866A1 (en) * 2004-09-24 2010-08-26 Alnylam Pharmaceuticals, Inc. RNAi Modulation of APOB and Uses Thereof
US20100234446A1 (en) * 2004-11-24 2010-09-16 Philipp Hadwiger RNAi Modulation of the BCR-ABL Fusion Gene and Uses Thereof
US20100240730A1 (en) * 2002-02-20 2010-09-23 Merck Sharp And Dohme Corp. RNA Interference Mediated Inhibition of Gene Expression Using Chemically Modified Short Interfering Nucleic Acid (siNA)
US20100240441A1 (en) * 2007-09-14 2010-09-23 Konami Digital Entertainment Co., Ltd Game system, and game apparatus and challenge notifying apparatus constituting the game system
US20100249052A1 (en) * 2007-03-26 2010-09-30 Alnylam Pharmaceuticals, Inc. Dsrna compositions and methods for treating hpv infections
US20100249212A1 (en) * 2000-08-02 2010-09-30 University Of Southern California Gene Silencing Using mRNA-cDNA Hybrids
US7807646B1 (en) 2003-11-20 2010-10-05 University Of South Florida SHIP-deficiency to increase megakaryocyte progenitor production
US20100256218A1 (en) * 2004-12-14 2010-10-07 Olaf Heidenreich RNAi MODULATION OF MLL-AF4 AND USES THEREOF
US20100255023A1 (en) * 2007-11-30 2010-10-07 Si-Yi Chen Dendritic cell vaccine compositions and uses of same
US7812149B2 (en) 1996-06-06 2010-10-12 Isis Pharmaceuticals, Inc. 2′-Fluoro substituted oligomeric compounds and compositions for use in gene modulations
US20100267810A1 (en) * 2005-08-18 2010-10-21 University Of Massachusetts Methods and compositions for treating neurological disease
US7819842B2 (en) 2006-11-21 2010-10-26 Medtronic, Inc. Chronically implantable guide tube for repeated intermittent delivery of materials or fluids to targeted tissue sites
US20100273863A1 (en) * 2009-04-24 2010-10-28 Board Of Regents, The University Of Texas System Modulation of Gene Expression Using Oligomers That Target Gene Regions Downstream of 3' Untranslated Regions
US20100278871A1 (en) * 2003-05-05 2010-11-04 Johns Hopkins University Anti-cancer dna vaccine employing plasmids encoding signal sequence, mutant oncoprotein antigen, and heat shock protein
US20100280102A1 (en) * 2003-06-13 2010-11-04 Alnylam Pharmaceuticals Double-stranded ribonucleic acid with increased effectiveness in an organism
US7829694B2 (en) 2002-11-26 2010-11-09 Medtronic, Inc. Treatment of neurodegenerative disease through intracranial delivery of siRNA
US20100286230A1 (en) * 2005-10-20 2010-11-11 Sylentis S.A.U. Modulation of trpv expression levels
US20100292305A1 (en) * 2005-06-27 2010-11-18 Akin Akinc RNAi MODULATION OF HIF-1 AND THERAPUTIC USES THEREOF
US20100291188A1 (en) * 2008-12-04 2010-11-18 Musc Foundation For Research Development Periostin Inhibitory Compositions for Myocardial Regeneration, Methods of Delivery, and Methods of Using Same
US20100298405A1 (en) * 2005-10-28 2010-11-25 Dinah Wen-Yee Sah Compositions And Methods For Inhibiting Expression Of Huntingtin Gene
US20100316703A1 (en) * 2000-12-01 2010-12-16 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V. Rna interference mediating small rna molecules
US20100330105A1 (en) * 2006-08-22 2010-12-30 John Hopkins University Anticancer Combination Therapies
US20110003882A1 (en) * 2006-05-19 2011-01-06 Alnylam Pharmaceuticals, Inc. RNAi Modulation of AHA and Therapeutic Uses Thereof
US20110003879A1 (en) * 2005-03-11 2011-01-06 Vincent Mark D Antisense oligonucleotides targeted to the coding region of thymidylate synthase and uses thereof
US20110015252A1 (en) * 2009-06-15 2011-01-20 Kevin Fitzgerald Lipid formulated dsrna targeting the pcsk9 gene
US20110020300A1 (en) * 2009-05-15 2011-01-27 Genentech, Inc. Compositions and methods for inhibiting expression of glucocorticoid receptor (gcr) genes
US7884086B2 (en) * 2004-09-08 2011-02-08 Isis Pharmaceuticals, Inc. Conjugates for use in hepatocyte free uptake assays
US20110034537A1 (en) * 2008-02-12 2011-02-10 De Fougerolles Antonin Compositions and methods for inhibiting expression of cd45 gene
US7888010B2 (en) 2004-05-28 2011-02-15 Asuragen, Inc. Methods and compositions involving microRNA
US20110038922A1 (en) * 2005-06-16 2011-02-17 Faron Pharmaceuticals Oy (A Finnish Company) Compounds for treating or preventing amine oxidase related diseases or disorders
US20110053226A1 (en) * 2008-06-13 2011-03-03 Riboxx Gmbh Method for enzymatic synthesis of chemically modified rna
US7902352B2 (en) 2005-05-06 2011-03-08 Medtronic, Inc. Isolated nucleic acid duplex for reducing huntington gene expression
US20110060031A1 (en) * 2007-03-29 2011-03-10 Alnylam Pharmaceuticals, Inc. Compositions And Methods For Inhibiting Expression Of A Gene From The Ebola Virus
WO2011035065A1 (en) 2009-09-17 2011-03-24 Nektar Therapeutics Monoconjugated chitosans as delivery agents for small interfering nucleic acids
US7923206B2 (en) * 2004-11-22 2011-04-12 Dharmacon, Inc. Method of determining a cellular response to a biological agent
US20110092565A1 (en) * 2003-06-09 2011-04-21 Alnylam Pharmaceuticals, Inc. Method of treating neurodegenerative disease
US20110098460A1 (en) * 2001-07-23 2011-04-28 Senesco Technologies, Inc. SiRNA Useful to Suppress expression of eIF-5A1
US7935811B2 (en) 2004-11-22 2011-05-03 Dharmacon, Inc. Apparatus and system having dry gene silencing compositions
US20110110483A1 (en) * 2009-11-06 2011-05-12 Searete Llc, A Limited Liability Corporation Of The State Of Delaware Methods and systems for migrating fuel assemblies in a nuclear fission reactor
US20110117088A1 (en) * 2004-05-12 2011-05-19 Simon Michael R Composition and method for introduction of rna interference sequences into targeted cells and tissues
US7951935B2 (en) 2002-11-14 2011-05-31 Dharmacon, Inc. siRNA targeting v-myc myelocytomatosis viral oncogene homolog (MYC)
US20110142917A1 (en) * 2008-06-06 2011-06-16 Egvenia Alpert Compositions and methods for treatment of ear disorders
US20110150897A1 (en) * 2006-10-11 2011-06-23 Meyer Thomas F Influenza targets
US20110160277A1 (en) * 2005-10-25 2011-06-30 Sylentis S.A.U. Modulation of 11 beta-hydroxysteriod dehydrogenase 1 expression for the treatment of ocular diseases
US20110166199A1 (en) * 2010-01-07 2011-07-07 National Cheng Kung University RNAi COMPOUND TARGETED TO THROMBOSPONDIN-1 AND APPLICATIONS THEREOF
US20110172286A1 (en) * 2007-07-10 2011-07-14 Neurim Pharmaceuticals (1991) Ltd. Cd44 splice variants in neurodegenerative diseases
US20110172291A1 (en) * 2003-09-12 2011-07-14 University Of Massachusetts Rna interference for the treatment of gain-of-function disorders
US20110184046A1 (en) * 2008-07-11 2011-07-28 Dinah Wen-Yee Sah Compositions And Methods For Inhibiting Expression Of GSK-3 Genes
US20110184041A1 (en) * 2007-08-17 2011-07-28 Richard Kremer PTHrP, ITS ISOFORMS AND ANTAGONIST THERETO IN THE DIAGNOSIS AND TREATMENT OF DISEASE
US7988668B2 (en) 2006-11-21 2011-08-02 Medtronic, Inc. Microsyringe for pre-packaged delivery of pharmaceuticals
US20110190380A1 (en) * 2008-10-23 2011-08-04 Elena Feinstein Methods for delivery of sirna to bone marrow cells and uses thereof
US20110201667A1 (en) * 2009-07-20 2011-08-18 Protiva Biotherapeutics, Inc. Compositions and methods for silencing ebola virus gene expression
US20110207796A1 (en) * 2008-02-13 2011-08-25 Elan Pharma International Limited Alpha-synuclein kinase
US20110213328A1 (en) * 2004-03-18 2011-09-01 Medtronic, Inc. Methods and Systems for Treatment of Neurological Diseases of the Central Nervous System
US20110230542A1 (en) * 2006-05-11 2011-09-22 Pamela Tan Compositions and Methods for Inhibiting Expression of the PCSK9 Gene
US20110245325A1 (en) * 2008-12-12 2011-10-06 Kureha Corporation Pharmaceutical composition for treatment of cancer and asthma
US8058069B2 (en) 2008-04-15 2011-11-15 Protiva Biotherapeutics, Inc. Lipid formulations for nucleic acid delivery
US8058255B2 (en) 2004-12-23 2011-11-15 Applied Biosystems, Llc Methods and compositions concerning siRNA's as mediators of RNA interference
US8058448B2 (en) 2004-04-05 2011-11-15 Alnylam Pharmaceuticals, Inc. Processes and reagents for sulfurization of oligonucleotides
US8071562B2 (en) 2007-12-01 2011-12-06 Mirna Therapeutics, Inc. MiR-124 regulated genes and pathways as targets for therapeutic intervention
US20120004403A1 (en) * 2002-02-20 2012-01-05 Leonid Beigelman RNA INTERFERENCE MEDIATED INHIBITION OF TNF AND TNF RECEPTOR GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
US8198427B1 (en) 2002-11-14 2012-06-12 Dharmacon, Inc. SiRNA targeting catenin, beta-1 (CTNNB1)
US8222221B2 (en) 2008-06-04 2012-07-17 The Board Of Regents Of The University Of Texas System Modulation of gene expression through endogenous small RNA targeting of gene promoters
US20120202215A1 (en) * 2005-11-24 2012-08-09 Jichi Medical University Mitochondrial function of prohibitin 2 (phb2)
US8258111B2 (en) 2008-05-08 2012-09-04 The Johns Hopkins University Compositions and methods related to miRNA modulation of neovascularization or angiogenesis
US8258112B2 (en) 2005-05-06 2012-09-04 Medtronic, Inc Methods and sequences to suppress primate huntington gene Expression
US8283333B2 (en) 2009-07-01 2012-10-09 Protiva Biotherapeutics, Inc. Lipid formulations for nucleic acid delivery
US8288354B2 (en) * 2005-12-28 2012-10-16 The Scripps Research Institute Natural antisense and non-coding RNA transcripts as drug targets
US8293719B2 (en) 2004-03-12 2012-10-23 Alnylam Pharmaceuticals, Inc. iRNA agents targeting VEGF
US8324366B2 (en) 2008-04-29 2012-12-04 Alnylam Pharmaceuticals, Inc. Compositions and methods for delivering RNAI using lipoproteins
US8324367B2 (en) 2006-11-03 2012-12-04 Medtronic, Inc. Compositions and methods for making therapies delivered by viral vectors reversible for safety and allele-specificity
US8334273B2 (en) 2007-12-10 2012-12-18 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of factor VII gene
US8361714B2 (en) 2007-09-14 2013-01-29 Asuragen, Inc. Micrornas differentially expressed in cervical cancer and uses thereof
DE102011118024A1 (de) 2011-08-01 2013-02-07 Technische Universität Dresden Inhibitor der Expression der Pro-Caspase 1
US8394947B2 (en) 2004-06-03 2013-03-12 Isis Pharmaceuticals, Inc. Positionally modified siRNA constructs
US20130065939A1 (en) * 2009-09-23 2013-03-14 Protiva Biotherapeutics, Inc. Compositions and methods for silencing genes expressed in cancer
US8444983B2 (en) 2009-03-23 2013-05-21 Quark Pharmaceuticals, Inc. Composition of anti-ENDO180 antibodies and methods of use for the treatment of cancer and fibrotic diseases
US20130131331A1 (en) * 2004-11-19 2013-05-23 Genecare Research Institute Co., Ltd. Cancer-cell-specific cytostatic agent
US8455455B1 (en) 2010-03-31 2013-06-04 Protiva Biotherapeutics, Inc. Compositions and methods for silencing genes involved in hemorrhagic fever
US8470792B2 (en) 2008-12-04 2013-06-25 Opko Pharmaceuticals, Llc. Compositions and methods for selective inhibition of VEGF
US20130224311A1 (en) * 2010-03-19 2013-08-29 University Of South Alabama Methods and compositions for the treatment of cancer
US8524680B2 (en) * 2002-02-01 2013-09-03 Applied Biosystems, Llc High potency siRNAS for reducing the expression of target genes
US8546554B2 (en) 2008-09-25 2013-10-01 Alnylam Pharmaceuticals, Inc. Lipid formulated compositions and methods for inhibiting expression of Serum Amyloid A gene
US8569474B2 (en) 2004-03-09 2013-10-29 Isis Pharmaceuticals, Inc. Double stranded constructs comprising one or more short strands hybridized to a longer strand
US8569256B2 (en) 2009-07-01 2013-10-29 Protiva Biotherapeutics, Inc. Cationic lipids and methods for the delivery of therapeutic agents
US8592570B2 (en) 2008-10-06 2013-11-26 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of an RNA from West Nile virus
US8604183B2 (en) 2002-11-05 2013-12-10 Isis Pharmaceuticals, Inc. Compositions comprising alternating 2′-modified nucleosides for use in gene modulation
US20130331435A1 (en) * 2002-05-23 2013-12-12 Isis Pharmaceuticals, Inc. Antisense modulation of kinesin-like 1 expression
US8618277B2 (en) 2002-02-20 2013-12-31 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US20140010893A1 (en) * 2010-05-12 2014-01-09 University Of South Carolina Methods for Affecting Homology-Directed DNA Double Stranded Break Repair
US8648185B2 (en) 2002-02-20 2014-02-11 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US20140057962A1 (en) * 2011-03-11 2014-02-27 Surinder K. Batra Compositions and Methods for the Treatment of Cancer
US20140057960A1 (en) * 2009-05-05 2014-02-27 Medical Diagnostic Laboratories, Llc Novel repressor on ifn-lambda promoter and sirna against zeb1 and blimp-1 to increase ifn-lambda gene activity
US8664189B2 (en) 2008-09-22 2014-03-04 Rxi Pharmaceuticals Corporation RNA interference in skin indications
US8703930B2 (en) 2004-04-09 2014-04-22 Genecare Research Institute Co., Ltd. Cancer cell-specific apoptosis-inducing agents that target chromosome stabilization-associated genes
US8722641B2 (en) 2010-01-29 2014-05-13 St. Jude Children's Research Hospital Oligonucleotides which inhibit p53 induction in response to cellular stress
US20140135379A1 (en) * 2007-06-29 2014-05-15 Niigata University Method of fixing and expressing physiologically active substance
US20140179768A1 (en) * 2011-06-21 2014-06-26 Alnylam Pharmaceuticals, Inc. ANGIOPOIETIN-LIKE 3 (ANGPTL3) iRNA COMPOSITIONS AND METHODS OF USE THEREOF
US8815818B2 (en) 2008-07-18 2014-08-26 Rxi Pharmaceuticals Corporation Phagocytic cell delivery of RNAI
US8822426B2 (en) 2009-05-05 2014-09-02 Beeologics Inc. Prevention and treatment of nosema disease in bees
US8859516B2 (en) 2009-09-15 2014-10-14 Alnylam Pharmaceuticals, Inc. Lipid formulated compositions and methods for inhibiting expression of Eg5 and VEGF genes
US20140315973A1 (en) * 2010-10-07 2014-10-23 Agency For Science, Technology And Research Parp-1 inhibitors
US8871730B2 (en) 2009-07-13 2014-10-28 Somagenics Inc. Chemical modification of short small hairpin RNAs for inhibition of gene expression
US20140364485A1 (en) * 2012-01-06 2014-12-11 University Of South Alabama Methods and compositions for the treatment of cancer
US8957198B2 (en) 2003-02-03 2015-02-17 Medtronic, Inc. Compositions, devices and methods for treatment of Huntington's disease through intracranial delivery of sirna
US8962584B2 (en) 2009-10-14 2015-02-24 Yissum Research Development Company Of The Hebrew University Of Jerusalem, Ltd. Compositions for controlling Varroa mites in bees
US9006417B2 (en) 2010-06-30 2015-04-14 Protiva Biotherapeutics, Inc. Non-liposomal systems for nucleic acid delivery
US9018187B2 (en) 2009-07-01 2015-04-28 Protiva Biotherapeutics, Inc. Cationic lipids and methods for the delivery of therapeutic agents
US9023820B2 (en) 2009-01-26 2015-05-05 Protiva Biotherapeutics, Inc. Compositions and methods for silencing apolipoprotein C-III expression
US9029338B2 (en) 2009-08-14 2015-05-12 Alnylam Pharmaceuticals, Inc. Lipid formulated compositions and methods for inhibiting expression of a gene from the ebola virus
US9051570B2 (en) 2007-05-22 2015-06-09 Arcturus Therapeutics, Inc. UNA oligomers for therapeutics
US9051567B2 (en) 2009-06-15 2015-06-09 Tekmira Pharmaceuticals Corporation Methods for increasing efficacy of lipid formulated siRNA
US9057069B2 (en) 2006-03-31 2015-06-16 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of Eg5 gene
US9068184B2 (en) 2011-06-21 2015-06-30 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibition of expression of protein C (PROC) genes
US9074211B2 (en) 2008-11-19 2015-07-07 Rxi Pharmaceuticals Corporation Inhibition of MAP4K4 through RNAI
US9080171B2 (en) 2010-03-24 2015-07-14 RXi Parmaceuticals Corporation Reduced size self-delivering RNAi compounds
US9085638B2 (en) 2007-03-07 2015-07-21 The Johns Hopkins University DNA vaccine enhancement with MHC class II activators
US20150202223A1 (en) * 2012-10-02 2015-07-23 The General Hospital Corporation Methods relating to dna-sensing pathway related conditions
US9089610B2 (en) 2008-08-19 2015-07-28 Nektar Therapeutics Complexes of small-interfering nucleic acids
US9095504B2 (en) 2010-03-24 2015-08-04 Rxi Pharmaceuticals Corporation RNA interference in ocular indications
US9096636B2 (en) 1996-06-06 2015-08-04 Isis Pharmaceuticals, Inc. Chimeric oligomeric compounds and their use in gene modulation
US9101643B2 (en) 2009-11-03 2015-08-11 Alnylam Pharmaceuticals, Inc. Lipid formulated compositions and methods for inhibiting expression of transthyretin (TTR)
US9133517B2 (en) 2005-06-28 2015-09-15 Medtronics, Inc. Methods and sequences to preferentially suppress expression of mutated huntingtin
US9139554B2 (en) 2008-10-09 2015-09-22 Tekmira Pharmaceuticals Corporation Amino lipids and methods for the delivery of nucleic acids
US9173894B2 (en) 2011-02-02 2015-11-03 Excaliard Pharamaceuticals, Inc. Method of treating keloids or hypertrophic scars using antisense compounds targeting connective tissue growth factor (CTGF)
US9181546B2 (en) 2004-12-17 2015-11-10 Beth Israel Deaconess Medical Center Compositions for bacterial mediated gene silencing and methods of using same
US9181551B2 (en) 2002-02-20 2015-11-10 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US9187746B2 (en) 2009-09-22 2015-11-17 Alnylam Pharmaceuticals, Inc. Dual targeting siRNA agents
US9200276B2 (en) 2009-06-01 2015-12-01 Halo-Bio Rnai Therapeutics, Inc. Polynucleotides for multivalent RNA interference, compositions and methods of use thereof
US9228186B2 (en) 2002-11-14 2016-01-05 Thermo Fisher Scientific Inc. Methods and compositions for selecting siRNA of improved functionality
US9228188B2 (en) 2011-06-21 2016-01-05 Alnylam Pharmaceuticals, Inc. Compositions and method for inhibiting hepcidin antimicrobial peptide (HAMP) or HAMP-related gene expression
US20160024504A1 (en) * 2013-03-15 2016-01-28 Constellation Pharmaceuticals, Inc. Treating th2-mediated diseases by inhibition of bromodomains
US20160032402A1 (en) * 2013-03-15 2016-02-04 Novartis Ag Biomarkers associated with brm inhibition
US9260715B2 (en) 2007-01-16 2016-02-16 The University Of Queensland Method of inducing an immune response
US9260471B2 (en) 2010-10-29 2016-02-16 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using short interfering nucleic acids (siNA)
US9273356B2 (en) 2006-05-24 2016-03-01 Medtronic, Inc. Methods and kits for linking polymorphic sequences to expanded repeat mutations
US9315813B2 (en) 2011-06-21 2016-04-19 Alnylam Pharmaceuticals, Inc Compositions and methods for inhibition of expression of apolipoprotein C-III (APOC3) genes
US9340786B2 (en) 2010-03-24 2016-05-17 Rxi Pharmaceuticals Corporation RNA interference in dermal and fibrotic indications
US9375440B2 (en) 2006-11-03 2016-06-28 Medtronic, Inc. Compositions and methods for making therapies delivered by viral vectors reversible for safety and allele-specificity
US9399775B2 (en) 2011-11-18 2016-07-26 Alnylam Pharmaceuticals, Inc. RNAi agents, compositions and methods of use thereof for treating transthyretin (TTR) associated diseases
US20160215285A1 (en) * 2012-12-30 2016-07-28 The Regents Of The University Of California Methods of modulating compliance of the trabecular meshwork
US9493774B2 (en) 2009-01-05 2016-11-15 Rxi Pharmaceuticals Corporation Inhibition of PCSK9 through RNAi
US9492386B2 (en) 2002-06-28 2016-11-15 Protiva Biotherapeutics, Inc. Liposomal apparatus and manufacturing methods
US9587240B2 (en) 2001-01-09 2017-03-07 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of a target gene
WO2017050848A1 (en) * 2015-09-21 2017-03-30 Oommen Oommen P Nucleic acid molecules with enhanced activity
US9611478B2 (en) 2011-02-03 2017-04-04 Mirna Therapeutics, Inc. Synthetic mimics of miR-124
US9644241B2 (en) 2011-09-13 2017-05-09 Interpace Diagnostics, Llc Methods and compositions involving miR-135B for distinguishing pancreatic cancer from benign pancreatic disease
US9642872B2 (en) 2010-09-30 2017-05-09 University Of Zurich Treatment of B-cell lymphoma with microRNA
US9657294B2 (en) 2002-02-20 2017-05-23 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US20170152513A1 (en) * 2008-09-15 2017-06-01 Children's Medical Center Corporation Modulation of bcl11a for treatment of hemoglobinopathies
US9719092B2 (en) 2002-11-14 2017-08-01 Thermo Fisher Scientific Inc. RNAi targeting CNTD2
US9719094B2 (en) 2002-11-14 2017-08-01 Thermo Fisher Scientific Inc. RNAi targeting SEC61G
US9745574B2 (en) 2009-02-04 2017-08-29 Rxi Pharmaceuticals Corporation RNA duplexes with single stranded phosphorothioate nucleotide regions for additional functionality
US9771586B2 (en) 2002-11-14 2017-09-26 Thermo Fisher Scientific Inc. RNAi targeting ZNF205
US9808479B2 (en) 2012-09-05 2017-11-07 Sylentis Sau SiRNA and their use in methods and compositions for the treatment and / or prevention of eye conditions
US9839649B2 (en) 2002-11-14 2017-12-12 Thermo Fisher Scientific Inc. Methods and compositions for selecting siRNA of improved functionality
US9856475B2 (en) 2014-03-25 2018-01-02 Arcturus Therapeutics, Inc. Formulations for treating amyloidosis
US9879266B2 (en) 2002-11-14 2018-01-30 Thermo Fisher Scientific Inc. Methods and compositions for selecting siRNA of improved functionality
US9982259B2 (en) 2014-03-25 2018-05-29 Arcturus Therapeutics, Inc. Transthyretin allele selective UNA oligomers for gene silencing
US10011837B2 (en) 2014-03-04 2018-07-03 Sylentis Sau SiRNAs and their use in methods and compositions for the treatment and/or prevention of eye conditions
US10011832B2 (en) 2012-09-05 2018-07-03 Sylentis Sau SiRNA and their use in methods and compositions for the treatment and/or prevention of eye conditions
US10011836B2 (en) 2002-11-14 2018-07-03 Thermo Fisher Scientific Inc. Methods and compositions for selecting siRNA of improved functionality
US20180195064A1 (en) * 2015-06-30 2018-07-12 Tadamitsu Kishimoto Novel therapeutic agent for a lung disease and/or method for screening for the same
US10060921B2 (en) 2014-08-29 2018-08-28 Alnylam Pharmaceuticals, Inc. Methods of treating transthyretin (TTR) mediated amyloidosis
US10131904B2 (en) 2008-02-11 2018-11-20 Rxi Pharmaceuticals Corporation Modified RNAi polynucleotides and uses thereof
US10208307B2 (en) 2015-07-31 2019-02-19 Alnylam Pharmaceuticals, Inc. Transthyretin (TTR) iRNA compositions and methods of use thereof for treating or preventing TTR-associated diseases
US10214745B2 (en) 2012-03-15 2019-02-26 The Scripps Research Institute Treatment of brain derived neurotrophic factor (BDNF) related diseases by inhibition of natural antisense transcript to BDNF
US10246709B2 (en) 2016-03-07 2019-04-02 Arrowhead Pharmaceuticals, Inc. Targeting ligands for therapeutic compounds
US10287588B2 (en) 2014-04-25 2019-05-14 The Childrens's Medical Center Corporation Compositions and methods to treating hemoglobinopathies
US10294474B2 (en) 2016-09-02 2019-05-21 Arrowhead Pharmaceuticals, Inc. Targeting ligands
US10378012B2 (en) 2014-07-29 2019-08-13 Monsanto Technology Llc Compositions and methods for controlling insect pests
US10421964B2 (en) 2015-07-23 2019-09-24 Arcturus Therapeutics, Inc. UNA oligomers and compositions for treating amyloidosis
US10478500B2 (en) 2014-10-10 2019-11-19 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibition of HAO1 (Hydroxyacid Oxidase 1 (Glycolate Oxidase)) gene expression
US10513703B2 (en) 2014-11-10 2019-12-24 Alnylam Pharmaceuticals, Inc. Hepatitis B virus (HBV) iRNA compositions and methods of use thereof
US10519447B2 (en) 2015-04-01 2019-12-31 Arcturus Therapeutics, Inc. Therapeutic UNA oligomers and uses thereof
US10570393B2 (en) 2015-04-13 2020-02-25 Alnylam Pharmaceuticals, Inc. Angiopoietin-like 3 (ANGPTL3) iRNA compositions and methods of use thereof
US10590416B2 (en) 2017-07-06 2020-03-17 Arrowhead Pharmaceuticals, Inc. RNAi agents for inhibiting expression of alpha-ENaC and methods of use
US10597676B2 (en) 2013-07-19 2020-03-24 Monsanto Technology Llc Compositions and methods for controlling Leptinotarsa
US10597657B2 (en) 2017-09-11 2020-03-24 Arrowhead Pharmaceuticals, Inc. RNAi agents and compositions for inhibiting expression of apolipoprotein C-III (APOC3)
US10653619B2 (en) 2009-03-23 2020-05-19 Medtronic, Inc. Drug depots for treatment of pain and inflammation
US10683500B2 (en) 2014-03-25 2020-06-16 Arcturus Therapeutics, Inc. UNA oligomers having reduced off-target effects in gene silencing
US10731157B2 (en) 2015-08-24 2020-08-04 Halo-Bio Rnai Therapeutics, Inc. Polynucleotide nanoparticles for the modulation of gene expression and uses thereof
US10808247B2 (en) 2015-07-06 2020-10-20 Phio Pharmaceuticals Corp. Methods for treating neurological disorders using a synergistic small molecule and nucleic acids therapeutic approach
US10900039B2 (en) 2014-09-05 2021-01-26 Phio Pharmaceuticals Corp. Methods for treating aging and skin disorders using nucleic acids targeting Tyr or MMP1
US10934550B2 (en) 2013-12-02 2021-03-02 Phio Pharmaceuticals Corp. Immunotherapy of cancer
US10968449B2 (en) 2015-01-22 2021-04-06 Monsanto Technology Llc Compositions and methods for controlling Leptinotarsa
US10982212B2 (en) 2003-06-12 2021-04-20 Alnylam Pharmaceuticals, Inc. Conserved HBV and HCV sequences useful for gene silencing
US11001845B2 (en) 2015-07-06 2021-05-11 Phio Pharmaceuticals Corp. Nucleic acid molecules targeting superoxide dismutase 1 (SOD1)
US11021707B2 (en) 2015-10-19 2021-06-01 Phio Pharmaceuticals Corp. Reduced size self-delivering nucleic acid compounds targeting long non-coding RNA
US11053500B2 (en) * 2013-08-28 2021-07-06 lonis Pharmaceuticals, Inc. Modulation of prekallikrein (PKK) expression
US20210238590A1 (en) * 2018-06-14 2021-08-05 University Of Utah Research Foundation Staufen1 regulating agents and associated methods
US11091770B2 (en) 2014-04-01 2021-08-17 Monsanto Technology Llc Compositions and methods for controlling insect pests
US11142766B2 (en) 2014-11-17 2021-10-12 Alnylam Pharmaceuticals, Inc. Apolipoprotein C3 (APOC3) iRNA compositions and methods of use thereof
US11198869B2 (en) * 2017-12-01 2021-12-14 The Texas A&M University System Angelman syndrome antisense treatment
US11261447B2 (en) 2017-07-13 2022-03-01 Alnylam Pharmaceuticals, Inc. Methods for inhibition of HAO1 (hydroxyacid oxidase 1 (glycolate oxidase)) gene expression
USRE48948E1 (en) 2008-04-18 2022-03-01 Warsaw Orthopedic, Inc. Clonidine compounds in a biodegradable polymer
US11261441B2 (en) 2017-03-29 2022-03-01 Bluebird Bio, Inc. Vectors and compositions for treating hemoglobinopathies
US11279934B2 (en) 2014-04-28 2022-03-22 Phio Pharmaceuticals Corp. Methods for treating cancer using nucleic acids targeting MDM2 or MYCN
US11312962B2 (en) 2015-07-10 2022-04-26 Ionis Pharmaceuticals, Inc. Modulators of diacyglycerol acyltransferase 2 (DGAT2)
US11324820B2 (en) 2017-04-18 2022-05-10 Alnylam Pharmaceuticals, Inc. Methods for the treatment of subjects having a hepatitis b virus (HBV) infection
US11492623B2 (en) 2018-08-13 2022-11-08 Alnylam Pharmaceuticals, Inc. Hepatitis B virus (HBV) dsRNA agent compositions and methods of use thereof
US11542493B2 (en) 2012-11-27 2023-01-03 The Children's Medical Center Corporation Targeting BCL11A distal regulatory elements for fetal hemoglobin reinduction
US11572543B2 (en) 2015-05-08 2023-02-07 The Children's Medical Center. Corporation Targeting BCL11A enhancer functional regions for fetal hemoglobin reinduction
US11591544B2 (en) 2020-11-25 2023-02-28 Akagera Medicines, Inc. Ionizable cationic lipids
US11613752B2 (en) 2014-05-01 2023-03-28 Ionis Pharmaceuticals, Inc. Compositions and methods for modulating PKK expression
US11613751B2 (en) 2021-03-04 2023-03-28 Alnylam Pharmaceuticals, Inc. Angiopoietin-like 3 (ANGPTL3) iRNA compositions and methods of use thereof
US11723912B2 (en) 2016-12-08 2023-08-15 University Of Utah Research Foundation Staufen1 agents and associated methods
US11788087B2 (en) 2017-05-25 2023-10-17 The Children's Medical Center Corporation BCL11A guide delivery
US11806360B2 (en) 2017-09-19 2023-11-07 Alnylam Pharmaceuticals, Inc. Compositions and methods for treating transthyretin (TTR) mediated amyloidosis
US11959081B2 (en) 2021-08-03 2024-04-16 Alnylam Pharmaceuticals, Inc. Transthyretin (TTR) iRNA compositions and methods of use thereof

Families Citing this family (732)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993023569A1 (en) * 1992-05-11 1993-11-25 Ribozyme Pharmaceuticals, Inc. Method and reagent for inhibiting viral replication
US20030206887A1 (en) * 1992-05-14 2003-11-06 David Morrissey RNA interference mediated inhibition of hepatitis B virus (HBV) using short interfering nucleic acid (siNA)
US5639647A (en) * 1994-03-29 1997-06-17 Ribozyme Pharmaceuticals, Inc. 2'-deoxy-2'alkylnucleotide containing nucleic acid
US20040219569A1 (en) * 1999-07-06 2004-11-04 Fruma Yehiely Gene identification method
CA2487328A1 (en) 1998-03-20 1999-09-30 Benitec Australia Ltd. Sirna for control of gene expression
AUPP249298A0 (en) 1998-03-20 1998-04-23 Ag-Gene Australia Limited Synthetic genes and genetic constructs comprising same I
JP2003525017A (ja) * 1998-04-20 2003-08-26 リボザイム・ファーマシューティカルズ・インコーポレーテッド 遺伝子発現を調節しうる新規な化学組成を有する核酸分子
EP2314700A1 (en) 1999-01-28 2011-04-27 Medical College of Georgia Research Institute, Inc Composition and method for in vivo and in vitro attenuation of gene expression using double stranded RNA
DE19956568A1 (de) 1999-01-30 2000-08-17 Roland Kreutzer Verfahren und Medikament zur Hemmung der Expression eines vorgegebenen Gens
US7601494B2 (en) 1999-03-17 2009-10-13 The University Of North Carolina At Chapel Hill Method of screening candidate compounds for susceptibility to biliary excretion
PT1914244E (pt) 1999-04-09 2013-07-26 Kyowa Hakko Kirin Co Ltd Processo para regular a actividade de moléculas funcionais sob o ponto de vista imunológico
US6656698B1 (en) * 1999-06-30 2003-12-02 Millennium Pharmaceuticals, Inc. 12832, a novel human kinase-like molecule and uses thereof
US6423885B1 (en) 1999-08-13 2002-07-23 Commonwealth Scientific And Industrial Research Organization (Csiro) Methods for obtaining modified phenotypes in plant cells
DE10160151A1 (de) * 2001-01-09 2003-06-26 Ribopharma Ag Verfahren zur Hemmung der Expression eines vorgegebenen Zielgens
US7829693B2 (en) * 1999-11-24 2010-11-09 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of a target gene
DE10100586C1 (de) 2001-01-09 2002-04-11 Ribopharma Ag Verfahren zur Hemmung der Expression eines Ziegens
US7179796B2 (en) 2000-01-18 2007-02-20 Isis Pharmaceuticals, Inc. Antisense modulation of PTP1B expression
WO2002081628A2 (en) * 2001-04-05 2002-10-17 Ribozyme Pharmaceuticals, Incorporated Modulation of gene expression associated with inflammation proliferation and neurite outgrowth, using nucleic acid based technologies
US20050032733A1 (en) * 2001-05-18 2005-02-10 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (SiNA)
US20080039414A1 (en) * 2002-02-20 2008-02-14 Sima Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US20030084471A1 (en) * 2000-03-16 2003-05-01 David Beach Methods and compositions for RNA interference
US8202846B2 (en) 2000-03-16 2012-06-19 Cold Spring Harbor Laboratory Methods and compositions for RNA interference
AU2001245793A1 (en) 2000-03-16 2001-09-24 Cold Spring Harbor Laboratory Methods and compositions for rna interference
DK2028278T3 (da) * 2000-03-30 2014-06-23 Univ Massachusetts RNA-sekvensspecifikke formidlere af RNA-interferens
US20030190635A1 (en) * 2002-02-20 2003-10-09 Mcswiggen James A. RNA interference mediated treatment of Alzheimer's disease using short interfering RNA
US6946292B2 (en) 2000-10-06 2005-09-20 Kyowa Hakko Kogyo Co., Ltd. Cells producing antibody compositions with increased antibody dependent cytotoxic activity
US7767802B2 (en) 2001-01-09 2010-08-03 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of anti-apoptotic genes
CA2369944A1 (en) * 2001-01-31 2002-07-31 Nucleonics Inc. Use of post-transcriptional gene silencing for identifying nucleic acid sequences that modulate the function of a cell
US20050288242A1 (en) * 2001-05-18 2005-12-29 Sirna Therapeutics, Inc. RNA interference mediated inhibition of RAS gene expression using short interfering nucleic acid (siNA)
US20050124566A1 (en) * 2001-05-18 2005-06-09 Sirna Therapeutics, Inc. RNA interference mediated inhibition of myostatin gene expression using short interfering nucleic acid (siNA)
US20080161256A1 (en) * 2001-05-18 2008-07-03 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using short interfering nucleic acid (siNA)
US20050233997A1 (en) * 2001-05-18 2005-10-20 Sirna Therapeutics, Inc. RNA interference mediated inhibition of matrix metalloproteinase 13 (MMP13) gene expression using short interfering nucleic acid (siNA)
WO2005014811A2 (en) * 2003-08-08 2005-02-17 Sirna Therapeutics, Inc. RNA INTERFERENCE MEDIATED INHIBITION OF XIAP GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
WO2003070197A2 (en) * 2002-02-20 2003-08-28 Sirna Therapeutics, Inc. RNA INTERFERENCE MEDIATED INHIBITION OF TGF-BETA AND TGF-BETA RECEPTOR GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
US20050222066A1 (en) * 2001-05-18 2005-10-06 Sirna Therapeutics, Inc. RNA interference mediated inhibition of vascular endothelial growth factor and vascular endothelial growth factor receptor gene expression using short interfering nucleic acid (siNA)
US20050159379A1 (en) * 2001-05-18 2005-07-21 Sirna Therapeutics, Inc RNA interference mediated inhibition of gastric inhibitory polypeptide (GIP) and gastric inhibitory polypeptide receptor (GIPR) gene expression using short interfering nucleic acid (siNA)
US20050233344A1 (en) * 2001-05-18 2005-10-20 Sirna Therapeutics, Inc. RNA interference mediated inhibition of platelet derived growth factor (PDGF) and platelet derived growth factor receptor (PDGFR) gene expression using short interfering nucleic acid (siNA)
US20050054596A1 (en) * 2001-11-30 2005-03-10 Mcswiggen James RNA interference mediated inhibition of vascular endothelial growth factor and vascular endothelial growth factor receptor gene expression using short interfering nucleic acid (siNA)
US20050191618A1 (en) * 2001-05-18 2005-09-01 Sirna Therapeutics, Inc. RNA interference mediated inhibition of human immunodeficiency virus (HIV) gene expression using short interfering nucleic acid (siNA)
US20050196767A1 (en) * 2001-05-18 2005-09-08 Sirna Therapeutics, Inc. RNA interference mediated inhibition of GRB2 associated binding protein (GAB2) gene expression using short interfering nucleic acis (siNA)
US20050148530A1 (en) 2002-02-20 2005-07-07 Sirna Therapeutics, Inc. RNA interference mediated inhibition of vascular endothelial growth factor and vascular endothelial growth factor receptor gene expression using short interfering nucleic acid (siNA)
US20050143333A1 (en) * 2001-05-18 2005-06-30 Sirna Therapeutics, Inc. RNA interference mediated inhibition of interleukin and interleukin receptor gene expression using short interfering nucleic acid (SINA)
US20050048529A1 (en) * 2002-02-20 2005-03-03 Sirna Therapeutics, Inc. RNA interference mediated inhibition of intercellular adhesion molecule (ICAM) gene expression using short interfering nucleic acid (siNA)
US20050176663A1 (en) * 2001-05-18 2005-08-11 Sima Therapeutics, Inc. RNA interference mediated inhibition of protein tyrosine phosphatase type IVA (PRL3) gene expression using short interfering nucleic acid (siNA)
US20050079610A1 (en) * 2001-05-18 2005-04-14 Sirna Therapeutics, Inc. RNA interference mediated inhibition of Fos gene expression using short interfering nucleic acid (siNA)
US20050136436A1 (en) * 2001-05-18 2005-06-23 Sirna Therapeutics, Inc. RNA interference mediated inhibition of G72 and D-amino acid oxidase (DAAO) gene expression using short interfering nucleic acid (siNA)
US9994853B2 (en) 2001-05-18 2018-06-12 Sirna Therapeutics, Inc. Chemically modified multifunctional short interfering nucleic acid molecules that mediate RNA interference
US20050256068A1 (en) 2001-05-18 2005-11-17 Sirna Therapeutics, Inc. RNA interference mediated inhibition of stearoyl-CoA desaturase (SCD) gene expression using short interfering nucleic acid (siNA)
EP1627061B1 (en) * 2001-05-18 2009-08-12 Sirna Therapeutics, Inc. RNA INTERFERENCE MEDIATED INHIBITION OF GENE EXPRESSION USING CHEMICALLY MODIFIED SHORT INTERFERING NUCLEIC ACID (siNA)
US7109165B2 (en) * 2001-05-18 2006-09-19 Sirna Therapeutics, Inc. Conjugates and compositions for cellular delivery
US20070042983A1 (en) * 2001-05-18 2007-02-22 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using short interfering nucleic acid (siNA)
US20050182007A1 (en) * 2001-05-18 2005-08-18 Sirna Therapeutics, Inc. RNA interference mediated inhibition of interleukin and interleukin receptor gene expression using short interfering nucleic acid (SINA)
US20050176666A1 (en) * 2001-05-18 2005-08-11 Sirna Therapeutics, Inc. RNA interference mediated inhibition of GPRA and AAA1 gene expression using short interfering nucleic acid (siNA)
US20050159380A1 (en) * 2001-05-18 2005-07-21 Sirna Therapeutics, Inc. RNA interference mediated inhibition of angiopoietin gene expression using short interfering nucleic acid (siNA)
US20050267058A1 (en) * 2001-05-18 2005-12-01 Sirna Therapeutics, Inc. RNA interference mediated inhibition of placental growth factor gene expression using short interfering nucleic acid (sINA)
US20050261219A1 (en) * 2001-05-18 2005-11-24 Sirna Therapeutics, Inc. RNA interference mediated inhibition of interleukin and interleukin receptor gene expression using short interfering nucleic acid (siNA)
US20080188430A1 (en) * 2001-05-18 2008-08-07 Sirna Therapeutics, Inc. RNA interference mediated inhibition of hypoxia inducible factor 1 (HIF1) gene expression using short interfering nucleic acid (siNA)
US20090299045A1 (en) * 2001-05-18 2009-12-03 Sirna Therapeutics, Inc. RNA Interference Mediated Inhibition Of Interleukin and Interleukin Gene Expression Using Short Interfering Nucleic Acid (siNA)
US20050282188A1 (en) * 2001-05-18 2005-12-22 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using short interfering nucleic acid (siNA)
US20050158735A1 (en) * 2001-05-18 2005-07-21 Sirna Therapeutics, Inc. RNA interference mediated inhibition of proliferating cell nuclear antigen (PCNA) gene expression using short interfering nucleic acid (siNA)
US20050196765A1 (en) * 2001-05-18 2005-09-08 Sirna Therapeutics, Inc. RNA interference mediated inhibition of checkpoint Kinase-1 (CHK-1) gene expression using short interfering nucleic acid (siNA)
US7517864B2 (en) 2001-05-18 2009-04-14 Sirna Therapeutics, Inc. RNA interference mediated inhibition of vascular endothelial growth factor and vascular endothelial growth factor receptor gene expression using short interfering nucleic acid (siNA)
US20050137155A1 (en) * 2001-05-18 2005-06-23 Sirna Therapeutics, Inc. RNA interference mediated treatment of Parkinson disease using short interfering nucleic acid (siNA)
US20050124569A1 (en) * 2001-05-18 2005-06-09 Sirna Therapeutics, Inc. RNA interference mediated inhibition of CXCR4 gene expression using short interfering nucleic acid (siNA)
US20050014172A1 (en) 2002-02-20 2005-01-20 Ivan Richards RNA interference mediated inhibition of muscarinic cholinergic receptor gene expression using short interfering nucleic acid (siNA)
US20070093437A1 (en) * 2001-05-18 2007-04-26 Sirna Therapeutics, Inc. Rna interference mediated inhibition of xiap gene expression using short interfering nucleic acid (sina)
US20050159378A1 (en) 2001-05-18 2005-07-21 Sirna Therapeutics, Inc. RNA interference mediated inhibition of Myc and/or Myb gene expression using short interfering nucleic acid (siNA)
WO2003070897A2 (en) * 2002-02-20 2003-08-28 Ribozyme Pharmaceuticals, Incorporated RNA INTERFERENCE MEDIATED INHIBITION OF TNF AND TNF RECEPTOR SUPERFAMILY GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
US20050159382A1 (en) * 2001-05-18 2005-07-21 Sirna Therapeutics, Inc. RNA interference mediated inhibition of polycomb group protein EZH2 gene expression using short interfering nucleic acid (siNA)
US20050233996A1 (en) * 2002-02-20 2005-10-20 Sirna Therapeutics, Inc. RNA interference mediated inhibition of hairless (HR) gene expression using short interfering nucleic acid (siNA)
US20060211642A1 (en) * 2001-05-18 2006-09-21 Sirna Therapeutics, Inc. RNA inteference mediated inhibition of hepatitis C virus (HVC) gene expression using short interfering nucleic acid (siNA)
US20060142225A1 (en) * 2001-05-18 2006-06-29 Sirna Therapeutics, Inc. RNA interference mediated inhibition of cyclin dependent kinase-2 (CDK2) gene expression using short interfering nucleic acid (siNA)
US20040198682A1 (en) * 2001-11-30 2004-10-07 Mcswiggen James RNA interference mediated inhibition of placental growth factor gene expression using short interfering nucleic acid (siNA)
US20050119212A1 (en) * 2001-05-18 2005-06-02 Sirna Therapeutics, Inc. RNA interference mediated inhibition of FAS and FASL gene expression using short interfering nucleic acid (siNA)
US20070270579A1 (en) * 2001-05-18 2007-11-22 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using short interfering nucleic acid (siNA)
US20050153914A1 (en) * 2001-05-18 2005-07-14 Sirna Therapeutics, Inc. RNA interference mediated inhibition of MDR P-glycoprotein gene expression using short interfering nucleic acid (siNA)
US20050203040A1 (en) * 2001-05-18 2005-09-15 Sirna Therapeutics, Inc. RNA interference mediated inhibition of vascular cell adhesion molecule (VCAM) gene expression using short interfering nucleic acid (siNA)
US20050176664A1 (en) * 2001-05-18 2005-08-11 Sirna Therapeutics, Inc. RNA interference mediated inhibition of cholinergic muscarinic receptor (CHRM3) gene expression using short interfering nucleic acid (siNA)
US20050196781A1 (en) * 2001-05-18 2005-09-08 Sirna Therapeutics, Inc. RNA interference mediated inhibition of STAT3 gene expression using short interfering nucleic acid (siNA)
US20030175950A1 (en) * 2001-05-29 2003-09-18 Mcswiggen James A. RNA interference mediated inhibition of HIV gene expression using short interfering RNA
US20050209180A1 (en) * 2001-05-18 2005-09-22 Sirna Therapeutics, Inc. RNA interference mediated inhibition of hepatitis C virus (HCV) expression using short interfering nucleic acid (siNA)
US20050164224A1 (en) * 2001-05-18 2005-07-28 Sirna Therapeutics, Inc. RNA interference mediated inhibition of cyclin D1 gene expression using short interfering nucleic acid (siNA)
US20050187174A1 (en) * 2001-05-18 2005-08-25 Sirna Therapeutics, Inc. RNA interference mediated inhibition of intercellular adhesion molecule (ICAM) gene expression using short interfering nucleic acid (siNA)
US20050287128A1 (en) * 2001-05-18 2005-12-29 Sirna Therapeutics, Inc. RNA interference mediated inhibition of TGF-beta and TGF-beta receptor gene expression using short interfering nucleic acid (siNA)
US20060148743A1 (en) * 2001-05-18 2006-07-06 Vasant Jadhav RNA interference mediated inhibition of histone deacetylase (HDAC) gene expression using short interfering nucleic acid (siNA)
US20040019001A1 (en) * 2002-02-20 2004-01-29 Mcswiggen James A. RNA interference mediated inhibition of protein typrosine phosphatase-1B (PTP-1B) gene expression using short interfering RNA
US20050164967A1 (en) * 2001-05-18 2005-07-28 Sirna Therapeutics, Inc. RNA interference mediated inhibition of platelet-derived endothelial cell growth factor (ECGF1) gene expression using short interfering nucleic acid (siNA)
US20050164968A1 (en) * 2001-05-18 2005-07-28 Sirna Therapeutics, Inc. RNA interference mediated inhibition of ADAM33 gene expression using short interfering nucleic acid (siNA)
EP1390472A4 (en) * 2001-05-29 2004-11-17 Sirna Therapeutics Inc NUCLEIC ACID TREATMENT OF DISEASES OR SIDES RELATED TO RAS, HER2 AND HIV LEVELS
US8008472B2 (en) 2001-05-29 2011-08-30 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of human immunodeficiency virus (HIV) gene expression using short interfering nucleic acid (siNA)
US20050019915A1 (en) * 2001-06-21 2005-01-27 Bennett C. Frank Antisense modulation of superoxide dismutase 1, soluble expression
CA2790034A1 (en) 2001-06-21 2003-01-03 Isis Pharmaceuticals, Inc. Antisense modulation of superoxide dismutase 1, soluble expression
BR0211111A (pt) 2001-07-12 2004-06-22 Univ Massachusetts Molécula de ácido nucleico isolada, vetor, célula hospedeira, transgene, precursor de rna engenheirado, animal transgênico não humano, e, método de induzir a interferência de ácido ribonucleico de um gene alvo em uma célula
US20030198627A1 (en) * 2001-09-01 2003-10-23 Gert-Jan Arts siRNA knockout assay method and constructs
WO2003035870A1 (de) * 2001-10-26 2003-05-01 Ribopharma Ag Medikament zur behandlung eines pankreaskarzinoms
DE10230996A1 (de) * 2001-10-26 2003-07-17 Ribopharma Ag Medikament zur Behandlung eines Pankreaskarzinoms
WO2003035876A1 (de) * 2001-10-26 2003-05-01 Ribopharma Ag Verwendung einer doppelsträngigen ribonukleinsäure zur behandlung einer infektion mit einem (+)-strang-rna-virus
WO2003035083A1 (de) * 2001-10-26 2003-05-01 Ribopharma Ag Medikament zur behandlung einer fibrotischen erkrankung durch rna interferenz
JP2005508396A (ja) * 2001-11-02 2005-03-31 イントラディグム、コーポレイション 核酸送達ビヒクルのための治療方法
US20070203333A1 (en) * 2001-11-30 2007-08-30 Mcswiggen James RNA interference mediated inhibition of vascular endothelial growth factor and vascular endothelial growth factor receptor gene expression using short interfering nucleic acid (siNA)
US20040138163A1 (en) * 2002-05-29 2004-07-15 Mcswiggen James RNA interference mediated inhibition of vascular edothelial growth factor and vascular edothelial growth factor receptor gene expression using short interfering nucleic acid (siNA)
US20050075304A1 (en) * 2001-11-30 2005-04-07 Mcswiggen James RNA interference mediated inhibition of vascular endothelial growth factor and vascular endothelial growth factor receptor gene expression using short interfering nucleic acid (siNA)
US7294504B1 (en) 2001-12-27 2007-11-13 Allele Biotechnology & Pharmaceuticals, Inc. Methods and compositions for DNA mediated gene silencing
JP4491240B2 (ja) * 2002-01-17 2010-06-30 ザ ユニバーシティ オブ ブリティッシュ コロンビア Ｉｇｆｂｐ−２およびｉｇｆｂｐ−５を阻害する二重特異性アンチセンスオリゴヌクレオチド、並びにこれらの使用方法
DE10202419A1 (de) 2002-01-22 2003-08-07 Ribopharma Ag Verfahren zur Hemmung der Expression eines durch eine Chromosomen-Aberration entstandenen Zielgens
US8067575B2 (en) 2002-02-20 2011-11-29 Merck, Sharp & Dohme Corp. RNA interference mediated inhibition of cyclin D1 gene expression using short interfering nucleic acid (siNA)
EP1430157B1 (en) * 2002-02-20 2011-08-10 Sirna Therapeutics, Inc. RNA INTERFERENCE MEDIATED INHIBITION OF HEPATITIS C VIRUS (HCV) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
US7935812B2 (en) 2002-02-20 2011-05-03 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of hepatitis C virus (HCV) expression using short interfering nucleic acid (siNA)
US8258288B2 (en) 2002-02-20 2012-09-04 Sirna Therapeutics, Inc. RNA interference mediated inhibition of respiratory syncytial virus (RSV) expression using short interfering nucleic acid (siNA)
WO2003106476A1 (en) * 2002-02-20 2003-12-24 Sirna Therapeutics, Inc Nucleic acid mediated inhibition of enterococcus infection and cytolysin toxin activity
US7893248B2 (en) 2002-02-20 2011-02-22 Sirna Therapeutics, Inc. RNA interference mediated inhibition of Myc and/or Myb gene expression using short interfering nucleic acid (siNA)
US20090192105A1 (en) 2002-02-20 2009-07-30 Sirna Therapeutics, Inc. RNA INTERFERENCE MEDIATED INHIBITION OF INTERCELLULAR ADHESION MOLECULE (ICAM) GENE EXPRESSION USING SHORT INTERFERING NUCELIC ACID (siNA)
JP2005517437A (ja) * 2002-02-20 2005-06-16 サーナ・セラピューティクス・インコーポレイテッド 短干渉核酸（ｓｉＮａ）を用いる表皮成長因子レセプター遺伝子発現のＲＮＡ干渉媒介性阻害
EP1495041A4 (en) * 2002-02-20 2006-02-01 Sirna Therapeutics Inc RNA interferon-mediated inhibition of gene expression of G72 and D-amino acid oxidase (DAAO) using short-term interfering nucleic acid (siNA)
US7897753B2 (en) 2002-02-20 2011-03-01 Sirna Therapeutics, Inc. RNA interference mediated inhibition of XIAP gene expression using short interfering nucleic acid (siNA)
US20050096284A1 (en) * 2002-02-20 2005-05-05 Sirna Therapeutics, Inc. RNA interference mediated treatment of polyglutamine (polyQ) repeat expansion diseases using short interfering nucleic acid (siNA)
US7667030B2 (en) 2002-02-20 2010-02-23 Sirna Therapeutics, Inc. RNA interference mediated inhibition of matrix metalloproteinase 13 (MMP13) gene expression using short interfering nucleic acid (siNA)
EP1463842A4 (en) * 2002-02-20 2006-01-25 Sirna Therapeutics Inc RNA-Interferent-mediated inhibition of protein gene expression for C-ALPHA (PKC-ALPHA) gene using SINA (Short Interfering Nucleic Acid)
US7897752B2 (en) 2002-02-20 2011-03-01 Sirna Therapeutics, Inc. RNA interference mediated inhibition of telomerase gene expression using short interfering nucleic acid (siNA)
US7700760B2 (en) 2002-02-20 2010-04-20 Sirna Therapeutics, Inc. RNA interference mediated inhibition of vascular cell adhesion molecule (VCAM) gene expression using short interfering nucleic acid (siNA)
US20090099117A1 (en) 2002-02-20 2009-04-16 Sirna Therapeutics, Inc. RNA INTERFERENCE MEDIATED INHIBITION OF MYOSTATIN GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
US7928220B2 (en) 2002-02-20 2011-04-19 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of stromal cell-derived factor-1 (SDF-1) gene expression using short interfering nucleic acid (siNA)
US7928219B2 (en) 2002-02-20 2011-04-19 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of placental growth factor gene expression using short interfering nucleic acid (SINA)
US20090253774A1 (en) 2002-02-20 2009-10-08 Sirna Therapeutics, Inc. RNA INTERFERENCE MEDIATED INHIBITION OF PLATELET DERIVED GROWTH FACTOR (PDGF) AND PLATELET DERIVED GROWTH FACTOR RECEPTOR (PDGFR) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
US7795422B2 (en) 2002-02-20 2010-09-14 Sirna Therapeutics, Inc. RNA interference mediated inhibition of hypoxia inducible factor 1 (HIF1) gene expression using short interfering nucleic acid (siNA)
AU2003219900A1 (en) * 2002-02-22 2003-09-09 James R. Eshleman Antigene locks and therapeutic uses thereof
JP4662714B2 (ja) * 2002-03-01 2011-03-30 セルテック アール アンド ディー インコーポレイテッド 骨密度を増減させる方法
US7274703B2 (en) * 2002-03-11 2007-09-25 3Com Corporation Stackable network units with resiliency facility
US7357928B2 (en) 2002-04-08 2008-04-15 University Of Louisville Research Foundation, Inc. Method for the diagnosis and prognosis of malignant diseases
US20040009946A1 (en) 2002-05-23 2004-01-15 Ceptyr, Inc. Modulation of PTP1B expression and signal transduction by RNA interference
WO2003099298A1 (en) * 2002-05-24 2003-12-04 Max-Planck Gesellschaft zur Förderung der Wissenschaften e.V. Rna interference mediating small rna molecules
US20040248094A1 (en) * 2002-06-12 2004-12-09 Ford Lance P. Methods and compositions relating to labeled RNA molecules that reduce gene expression
AU2003276666A1 (en) * 2002-06-12 2003-12-31 Ambion, Inc. Methods and compositions relating to polypeptides with rnase iii domains that mediate rna interference
US20100075423A1 (en) * 2002-06-12 2010-03-25 Life Technologies Corporation Methods and compositions relating to polypeptides with rnase iii domains that mediate rna interference
JP2005529959A (ja) * 2002-06-14 2005-10-06 マイラス コーポレイション 細胞へのポリヌクレオチドの伝達をするための新規な方法
US20100009856A1 (en) * 2002-06-21 2010-01-14 Sinogenomax Sompany LTD. Randomized dna libraries and double-stranded rna libraries, use and method of production thereof
US8101348B2 (en) * 2002-07-10 2012-01-24 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V. RNA-interference by single-stranded RNA molecules
PT1857547T (pt) * 2002-08-05 2018-02-08 Silence Therapeutics Gmbh Formas adicionais novas de moléculas de rna de interferência
US20080274989A1 (en) 2002-08-05 2008-11-06 University Of Iowa Research Foundation Rna Interference Suppression of Neurodegenerative Diseases and Methods of Use Thereof
EP2258847B2 (en) 2002-08-05 2020-07-01 Silence Therapeutics GmbH Futher novel forms of interfering RNA molecules
AU2012216354B2 (en) * 2002-08-05 2016-01-14 Silence Therapeutics Gmbh Further novel forms of interfering RNA molecules
EP1389637B1 (en) 2002-08-05 2012-05-30 Silence Therapeutics Aktiengesellschaft Blunt-ended interfering RNA molecules
US20050042646A1 (en) 2002-08-05 2005-02-24 Davidson Beverly L. RNA interference suppresion of neurodegenerative diseases and methods of use thereof
AU2015264957B2 (en) * 2002-08-05 2017-10-26 Silence Therapeutics Gmbh Further novel forms of interfering rna molecules
US20040241854A1 (en) 2002-08-05 2004-12-02 Davidson Beverly L. siRNA-mediated gene silencing
US20040029275A1 (en) * 2002-08-10 2004-02-12 David Brown Methods and compositions for reducing target gene expression using cocktails of siRNAs or constructs expressing siRNAs
IL166718A0 (en) * 2002-08-14 2006-01-15 Atugen Ag Further use of protein kinase n beta
US20080260744A1 (en) 2002-09-09 2008-10-23 Omeros Corporation G protein coupled receptors and uses thereof
CA2500224C (en) 2002-09-25 2015-04-28 University Of Massachusetts In vivo gene silencing by chemically modified and stable sirna
AU2003279010A1 (en) * 2002-09-28 2004-04-19 Massachusetts Institute Of Technology Compositions and methods for delivery of short interfering rna and short hairpin rna
US20060160759A1 (en) * 2002-09-28 2006-07-20 Jianzhu Chen Influenza therapeutic
EP1562971B1 (en) 2002-11-05 2014-02-12 Isis Pharmaceuticals, Inc. Polycyclic sugar surrogate-containing oligomeric compounds and compositions for use in gene modulation
WO2004043978A2 (en) * 2002-11-05 2004-05-27 Isis Pharmaceuticals, Inc. 2'-methoxy substituted oligomeric compounds and compositions for use in gene modulations
US9150606B2 (en) 2002-11-05 2015-10-06 Isis Pharmaceuticals, Inc. Compositions comprising alternating 2'-modified nucleosides for use in gene modulation
US9150605B2 (en) 2002-11-05 2015-10-06 Isis Pharmaceuticals, Inc. Compositions comprising alternating 2′-modified nucleosides for use in gene modulation
DE10322662A1 (de) * 2002-11-06 2004-10-07 Grünenthal GmbH Wirksame und stabile DNA-Enzyme
US7250496B2 (en) 2002-11-14 2007-07-31 Rosetta Genomics Ltd. Bioinformatically detectable group of novel regulatory genes and uses thereof
US10920226B2 (en) * 2002-11-14 2021-02-16 Thermo Fisher Scientific Inc. siRNA targeting LDHA
US7655785B1 (en) 2002-11-14 2010-02-02 Rosetta Genomics Ltd. Bioinformatically detectable group of novel regulatory oligonucleotides and uses thereof
US8163896B1 (en) 2002-11-14 2012-04-24 Rosetta Genomics Ltd. Bioinformatically detectable group of novel regulatory genes and uses thereof
AU2003303308B2 (en) 2002-11-18 2009-07-30 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services, Centers For Disease Control And Prevention Cell lines and host nucleic acid sequences related to infectious disease
US7064337B2 (en) 2002-11-19 2006-06-20 The Regents Of The University Of California Radiation detection system for portable gamma-ray spectroscopy
DE10254214A1 (de) * 2002-11-20 2004-06-09 Beiersdorf Ag Oligoribonukleotide zur Behandlung von degenerativen Hauterscheinungen durch RNA-Interferenz
WO2004048566A1 (ja) * 2002-11-22 2004-06-10 Bio-Think Tank Co., Ltd. Ｒｎａ干渉の標的塩基配列検索方法、ｒｎａ干渉を生じさせるポリヌクレオチドの塩基配列設計方法、２本鎖ポリヌクレオチドの作製方法、遺伝子の発現抑制方法、塩基配列処理装置、塩基配列処理方法をコンピュータに実行させるプログラム、記録媒体、および、塩基配列処理システム
JP4526228B2 (ja) * 2002-11-22 2010-08-18 隆 森田 ＲＮＡｉによる新規治療法および治療剤
US7790867B2 (en) 2002-12-05 2010-09-07 Rosetta Genomics Inc. Vaccinia virus-related nucleic acids and microRNA
EP1585756B1 (en) * 2002-11-26 2010-04-21 University of Massachusetts Delivery of sirnas
US20130130231A1 (en) 2002-11-26 2013-05-23 Isaac Bentwich Bioinformatically detectable group of novel viral regulatory genes and uses thereof
US7696334B1 (en) 2002-12-05 2010-04-13 Rosetta Genomics, Ltd. Bioinformatically detectable human herpesvirus 5 regulatory gene
CN1301263C (zh) 2002-12-18 2007-02-21 北京昭衍新药研究中心 一组抗hiv感染及防治艾滋病的核苷酸序列及其应用
NZ540730A (en) 2002-12-24 2010-09-30 Rinat Neuroscience Corp Anti-NGF antibodies and methods using same
US9498530B2 (en) 2002-12-24 2016-11-22 Rinat Neuroscience Corp. Methods for treating osteoarthritis pain by administering a nerve growth factor antagonist and compositions containing the same
EP2251434B1 (en) 2003-01-16 2014-07-30 The Trustees of The University of Pennsylvania Compositions and methods for siRNA inhibition of ICAM-1
US7629323B2 (en) * 2003-01-21 2009-12-08 Northwestern University Manipulation of neuronal ion channels
AU2004211949A1 (en) * 2003-02-05 2004-08-26 University Of Massachusetts RNAi targeting of viruses
US20070104688A1 (en) 2003-02-13 2007-05-10 City Of Hope Small interfering RNA mediated transcriptional gene silencing in mammalian cells
US20040162235A1 (en) * 2003-02-18 2004-08-19 Trubetskoy Vladimir S. Delivery of siRNA to cells using polyampholytes
ATE491444T1 (de) 2003-02-19 2011-01-15 Rinat Neuroscience Corp Verfahren zur behandlung von schmerzen durch verabreichung eines nervenwachstumsfaktor- antagonisten und eines nsaid und diese enthaltende zusammensetzung
AU2004214954A1 (en) 2003-02-27 2004-09-10 Alnylam Pharmaceuticals, Inc. Methods and constructs for evaluation of RNAi targets and effector molecules
KR20050103305A (ko) * 2003-02-27 2005-10-28 내셔날 인스티튜트 오브 어드밴스드 인더스트리얼 사이언스 앤드 테크놀로지 포유 동물 세포에 있어서의 ｄｓＲＮＡ에 의한 ＣｐＧ서열로의 메틸화 유도
CA2518475C (en) 2003-03-07 2014-12-23 Alnylam Pharmaceuticals, Inc. Irna agents comprising asymmetrical modifications
AU2004221760B2 (en) 2003-03-21 2010-03-18 Roche Innovation Center Copenhagen A/S Short interfering RNA (siRNA) analogues
US20040198640A1 (en) * 2003-04-02 2004-10-07 Dharmacon, Inc. Stabilized polynucleotides for use in RNA interference
ATE536408T1 (de) * 2003-04-02 2011-12-15 Dharmacon Inc Modifizierte polynukleotide zur verwendung bei rna-interferenz
EP1608733B1 (en) * 2003-04-02 2011-12-07 Dharmacon, Inc. Modified polynucleotides for use in rna interference
CA2521464C (en) 2003-04-09 2013-02-05 Alnylam Pharmaceuticals, Inc. Irna conjugates
EP1631669A2 (en) 2003-04-09 2006-03-08 Biodelivery Sciences International, Inc. Cochleate compositions directed against expression of proteins
WO2004092383A2 (en) * 2003-04-15 2004-10-28 Sirna Therapeutics, Inc. RNA INTERFERENCE MEDIATED INHIBITION OF SEVERE ACUTE RESPIRATORY SYNDROME (SARS) VIRUS GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
US8017762B2 (en) 2003-04-17 2011-09-13 Alnylam Pharmaceuticals, Inc. Modified iRNA agents
US8796436B2 (en) 2003-04-17 2014-08-05 Alnylam Pharmaceuticals, Inc. Modified iRNA agents
US7723509B2 (en) 2003-04-17 2010-05-25 Alnylam Pharmaceuticals IRNA agents with biocleavable tethers
US7851615B2 (en) 2003-04-17 2010-12-14 Alnylam Pharmaceuticals, Inc. Lipophilic conjugated iRNA agents
EP1620544B1 (en) 2003-04-17 2018-09-19 Alnylam Pharmaceuticals Inc. MODIFIED iRNA AGENTS
CA2522349A1 (en) 2003-04-17 2004-11-04 Alnylam Pharmaceuticals, Inc. Protected monomers
EP1623032A2 (en) 2003-05-09 2006-02-08 University of Pittsburgh of the Commonwealth System of Higher Education Small interfering rna libraries and methods of synthesis and use
ATE516047T1 (de) 2003-05-09 2011-07-15 Diadexus Inc Ovr110-antikörperzusammensetzungen und anwendungsverfahren
AU2003241409A1 (en) * 2003-05-12 2005-01-21 Potomac Pharmaceuticals, Inc. Gene expression suppression agents
US20050148531A1 (en) * 2003-05-15 2005-07-07 Todd Hauser Modulation of gene expression using DNA-DNA hybrids
KR20060013426A (ko) 2003-05-30 2006-02-09 니뽄 신야쿠 가부시키가이샤 Ｂｃｌ-2의 발현을 억제하는 올리고 이중쇄 ＲＮＡ와그것을 함유하는 의약 조성물
US20070042979A1 (en) * 2003-05-30 2007-02-22 Junichi Yano Oligonucleic acid-bearing composite and pharmaceutical composition containing the composite
EP1486564A1 (de) * 2003-06-13 2004-12-15 Ribopharma AG SiRNA mit erhöhter Stabilität in Serum
EP1644475A4 (en) * 2003-06-20 2009-06-03 Isis Pharmaceuticals Inc DOUBLE-STRAND COMPOSITIONS WITH A 3'-ENDO-MODIFIED STRING FOR USE IN GENE MODULATION
EP2371835A1 (en) * 2003-07-03 2011-10-05 The Trustees Of The University Of Pennsylvania Inhibition of syk kinase expression
US20050079614A1 (en) * 2003-07-21 2005-04-14 Reinhart Brenda J. RNAs able to modulate chromatin silencing
CA2533701A1 (en) 2003-07-31 2005-02-17 Isis Pharmaceuticals, Inc. Oligomeric compounds and compositions for use in modulation of small non-coding rnas
US20060229266A1 (en) * 2003-08-13 2006-10-12 Kumar Nalin M Silencing of tgf-beta receptor type II expression by sirna
US7888497B2 (en) 2003-08-13 2011-02-15 Rosetta Genomics Ltd. Bioinformatically detectable group of novel regulatory oligonucleotides and uses thereof
WO2005035759A2 (en) * 2003-08-20 2005-04-21 Sirna Therapeutics, Inc. RNA INTERFERENCE MEDIATED INHIBITION OF HYPOXIA INDUCIBLE FACTOR 1 (HIF1) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
US8501705B2 (en) * 2003-09-11 2013-08-06 The Board Of Regents Of The University Of Texas System Methods and materials for treating autoimmune and/or complement mediated diseases and conditions
US7425544B2 (en) 2003-09-18 2008-09-16 Eli Lilly And Company Modulation of eIF4E expression
WO2005031002A2 (en) * 2003-09-22 2005-04-07 Rosetta Inpharmatics Llc Synthetic lethal screen using rna interference
WO2005033310A1 (de) * 2003-10-01 2005-04-14 Grünenthal GmbH Pim-1-spezifische dsrna-verbindungen
CA2541970A1 (en) 2003-10-09 2005-06-09 E.I. Du Pont De Nemours And Company Gene silencing by using micro-rna molecules
WO2005045032A2 (en) * 2003-10-20 2005-05-19 Sima Therapeutics, Inc. RNA INTERFERENCE MEDIATED INHIBITION OF EARLY GROWTH RESPONSE GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
WO2005045035A2 (en) * 2003-10-23 2005-05-19 Sirna Therapeutics, Inc. RNA INTERFERENCE MEDIATED INHIBITION OF NOGO AND NOGO RECEPTOR GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
EP1694838A2 (en) * 2003-10-23 2006-08-30 Sirna Therapeutics, Inc. RNA INTERFERENCE MEDIATED INHIBITION OF GPRA AND AAA1 GENE EXPRESSION USING SHORT NUCLEIC ACID (siNA)
WO2005042708A2 (en) 2003-10-27 2005-05-12 Rosetta Inpharmatics Llc METHOD OF DESIGNING siRNAS FOR GENE SILENCING
US8227434B1 (en) 2003-11-04 2012-07-24 H. Lee Moffitt Cancer Center & Research Institute, Inc. Materials and methods for treating oncological disorders
WO2005047504A1 (en) * 2003-11-07 2005-05-26 The Board Of Trustees Of The University Of Illinois Induction of cellular senescence by cdk4 disruption for tumor suppression and regression
JP2005168485A (ja) * 2003-11-20 2005-06-30 Tsutomu Suzuki ｓｉＲＮＡの設計方法
US20100145038A1 (en) * 2003-11-24 2010-06-10 Merck & Co., Inc. RNA INTERFERENCE MEDIATED INHIBITION OF GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
JPWO2005068630A1 (ja) * 2003-12-16 2007-07-26 独立行政法人産業技術総合研究所 干渉用二重鎖ｒｎａ
EP1713912B1 (en) * 2004-01-30 2013-09-18 Santaris Pharma A/S Modified short interfering rna (modified sirna)
DE602005017362D1 (de) * 2004-02-06 2009-12-10 Dharmacon Inc Stabilisierte rnas als transfektionskontrollen und silencing-reagentien
US20060019914A1 (en) 2004-02-11 2006-01-26 University Of Tennessee Research Foundation Inhibition of tumor growth and invasion by anti-matrix metalloproteinase DNAzymes
US20070087008A1 (en) 2004-02-24 2007-04-19 The Government Of The United States Of America As Rab9a, rab11a, and modulators thereof related to infectious disease
US7691823B2 (en) * 2004-03-05 2010-04-06 University Of Massachusetts RIP140 regulation of glucose transport
JPWO2005095647A1 (ja) * 2004-03-31 2008-02-21 タカラバイオ株式会社 ｓｉＲＮＡのスクリーニング方法
KR101147147B1 (ko) * 2004-04-01 2012-05-25 머크 샤프 앤드 돔 코포레이션 Ｒｎａ 간섭의 오프 타겟 효과 감소를 위한 변형된폴리뉴클레오타이드
WO2005105157A2 (en) 2004-04-23 2005-11-10 The Trustees Of Columbia University In The City Ofnew York INHIBITION OF HAIRLESS PROTEIN mRNA
CA2564868C (en) 2004-04-28 2013-11-26 Molecules For Health, Inc. Methods for treating or preventing restenosis and other vascular proliferative disorders
WO2005110464A2 (en) * 2004-05-14 2005-11-24 Oregon Health & Science University Irx5 inhibition as treatment for hyperproliferative disorders
US7687616B1 (en) 2004-05-14 2010-03-30 Rosetta Genomics Ltd Small molecules modulating activity of micro RNA oligonucleotides and micro RNA targets and uses thereof
US10508277B2 (en) 2004-05-24 2019-12-17 Sirna Therapeutics, Inc. Chemically modified multifunctional short interfering nucleic acid molecules that mediate RNA interference
US7964196B2 (en) * 2004-05-25 2011-06-21 Chimeros, Inc. Self-assembling nanoparticle drug delivery system
EP1602926A1 (en) 2004-06-04 2005-12-07 University of Geneva Novel means and methods for the treatment of hearing loss and phantom hearing
US20090215860A1 (en) * 2004-06-17 2009-08-27 The Regents Of The University Of California Compositions and methods for regulating gene transcription
JP2008507341A (ja) * 2004-07-21 2008-03-13 メドトロニック，インコーポレイティド 限局性繊維症を低減するための医療装置及び方法
CA2573671A1 (en) * 2004-07-21 2006-02-23 Medtronic, Inc. Methods for reducing or preventing localized fibrosis using sirna
EP1782321A4 (en) 2004-07-23 2009-11-04 Univ North Carolina METHOD AND MATERIALS FOR DETERMINING PAIN SENSITIVITY AND PREDICTING AND TREATING SUFFERING INTERFERENCE
WO2006020768A2 (en) 2004-08-10 2006-02-23 Alnylam Pharmaceuticals, Inc. Chemically modified oligonucleotides
EP1791568B1 (en) 2004-08-16 2012-08-15 Quark Pharmaceuticals, Inc. Therapeutic uses of inhibitors of rtp801
US7323310B2 (en) 2004-08-31 2008-01-29 Qiagen North American Holdings, Inc. Methods and compositions for RNA amplification and detection using an RNA-dependent RNA-polymerase
FI20041204A0 (fi) 2004-09-16 2004-09-16 Riikka Lund Menetelmät immuunivälitteisiin sairauksiin liittyvien uusien kohdegeenien hyödyntämiseksi
LT1799269T (lt) 2004-09-28 2016-10-25 Quark Pharmaceuticals, Inc. Oligoribonukleotidai ir jų naudojimo būdai alopecijos, ūminio inkstų nepakankamumo ir kitų ligų gydymui
EP2336333A1 (en) 2004-10-21 2011-06-22 Venganza Inc. Methods and materials for conferring resistance to pests and pathogens of plants
US20060110440A1 (en) * 2004-10-22 2006-05-25 Kiminobu Sugaya Method and system for biasing cellular development
MX2007005069A (es) * 2004-10-27 2007-06-25 Schering Corp Composiciones y metodos para la inhibicion del acido nucleico corto de interferencia de nav1.8.
EP1812597A2 (en) 2004-10-27 2007-08-01 Vanderbilt University Mammalian genes involved in infection
WO2006050002A2 (en) * 2004-10-28 2006-05-11 Idexx Laboratories, Inc. Compositions for controlled delivery of pharmaceutically active compounds
US9492400B2 (en) 2004-11-04 2016-11-15 Massachusetts Institute Of Technology Coated controlled release polymer particles as efficient oral delivery vehicles for biopharmaceuticals
WO2008073922A2 (en) * 2006-12-08 2008-06-19 Asuragen, Inc. Functions and targets of let-7 micro rnas
US20060105052A1 (en) * 2004-11-15 2006-05-18 Acar Havva Y Cationic nanoparticle having an inorganic core
CA2587854C (en) * 2004-11-18 2014-01-21 The Board Of Trustees Of The University Of Illinois Multicistronic sirna constructs to inhibit tumors
US7923207B2 (en) 2004-11-22 2011-04-12 Dharmacon, Inc. Apparatus and system having dry gene silencing pools
US20060160110A1 (en) * 2004-12-02 2006-07-20 Takayuki Mizutani Methods of designing small interfering RNAs, antisense polynucleotides, and other hybridizing polynucleotides
US20060165667A1 (en) * 2004-12-03 2006-07-27 Case Western Reserve University Novel methods, compositions and devices for inducing neovascularization
GB0427916D0 (en) * 2004-12-21 2005-01-19 Astrazeneca Ab Method
TWI386225B (zh) 2004-12-23 2013-02-21 Alcon Inc 用於治療眼睛病症的結締組織生長因子（ＣＴＧＦ）ＲＮＡ干擾（ＲＮＡｉ）抑制技術
KR101366482B1 (ko) 2004-12-27 2014-02-21 사일런스 테라퓨틱스 아게 코팅된 지질 복합체 및 이들의 용도
BRPI0519690A2 (pt) * 2004-12-30 2009-03-03 Todd M Hauser composiÇÕes e mÉtodos para modular a expressço de genes usando oligonucleotÍdeos autoprotegidos
US7964195B2 (en) 2005-01-07 2011-06-21 Diadexus, Inc. Ovr110 antibody compositions and methods of use
US8088579B2 (en) 2005-02-14 2012-01-03 University Of Iowa Research Foundation Complement factor H for diagnosis of age-related macular degeneration
EP2157182A3 (en) * 2005-03-08 2012-04-25 Qiagen GmbH Modified short interfering RNA
GB0505081D0 (en) * 2005-03-14 2005-04-20 Genomica Sau Downregulation of interleukin-12 expression by means of rnai technology
DK1866414T3 (da) * 2005-03-31 2012-04-23 Calando Pharmaceuticals Inc Inhibitorer af ribonukleotidreduktase-underenhed 2 og anvendelser deraf.
US9505867B2 (en) * 2005-05-31 2016-11-29 Ecole Polytechmique Fédérale De Lausanne Triblock copolymers for cytoplasmic delivery of gene-based drugs
WO2006130560A2 (en) * 2005-05-31 2006-12-07 The Trustees Of The University Of Pennsylvania Manipulation of pten in t cells as a strategy to modulate immune responses
CA2610267A1 (en) * 2005-06-01 2006-12-07 Duke University Method of inhibiting intimal hyperplasia
DK1888749T3 (da) 2005-06-01 2015-01-05 Polyplus Transfection Oligonukleotider til RNA-interferens samt biologiske anvendelser deraf
CN100445381C (zh) * 2005-06-10 2008-12-24 中国人民解放军军事医学科学院基础医学研究所 带有单链polyA尾巴的siRNA分子制备方法和应用
US20100266574A1 (en) * 2005-06-10 2010-10-21 Orna Mor Oligoribonucleotides and Methods of Use Thereof for Treatment of Fibrotic Conditions and Other Diseases
WO2006138145A1 (en) 2005-06-14 2006-12-28 Northwestern University Nucleic acid functionalized nanoparticles for therapeutic applications
WO2007011702A2 (en) 2005-07-15 2007-01-25 The University Of North Carolina At Chapel Hill Use of egfr inhibitors to prevent or treat obesity
US7919583B2 (en) 2005-08-08 2011-04-05 Discovery Genomics, Inc. Integration-site directed vector systems
US20070213257A1 (en) * 2005-08-12 2007-09-13 Nastech Pharmaceutical Company Inc. Compositions and methods for complexes of nucleic acids and peptides
WO2007028065A2 (en) 2005-08-30 2007-03-08 Isis Pharmaceuticals, Inc. Chimeric oligomeric compounds for modulation of splicing
WO2007030167A1 (en) * 2005-09-02 2007-03-15 Nastech Pharmaceutical Company Inc. Modification of double-stranded ribonucleic acid molecules
US20090221673A1 (en) * 2005-09-13 2009-09-03 Rigby William F C Compositions and Methods for Regulating RNA Translation via CD154 CA-Dinucleotide Repeat
WO2007039454A1 (en) 2005-09-20 2007-04-12 Basf Plant Science Gmbh Methods for controlling gene expression using ta-siran
FR2890859B1 (fr) * 2005-09-21 2012-12-21 Oreal Oligonucleotide d'arn double brin inhibant l'expression de la tyrosinase
US8168584B2 (en) 2005-10-08 2012-05-01 Potentia Pharmaceuticals, Inc. Methods of treating age-related macular degeneration by compstatin and analogs thereof
US8080534B2 (en) 2005-10-14 2011-12-20 Phigenix, Inc Targeting PAX2 for the treatment of breast cancer
WO2007049690A1 (ja) * 2005-10-27 2007-05-03 National University Corporation NARA Institute of Science and Technology Singarの発現または機能の抑制による神経軸索の形成・伸長と神経再生への応用
US8916530B2 (en) 2005-11-18 2014-12-23 Gradalis, Inc. Individualized cancer therapy
US8603991B2 (en) 2005-11-18 2013-12-10 Gradalis, Inc. Individualized cancer therapy
US9267937B2 (en) 2005-12-15 2016-02-23 Massachusetts Institute Of Technology System for screening particles
JP2009521234A (ja) * 2005-12-22 2009-06-04 エクセジェニックス、インク．ディー／ビー／エー オプコ ヘルス、インク． 補体系を制御する組成および方法
AR057252A1 (es) * 2005-12-27 2007-11-21 Alcon Mfg Ltd Inhibicion de rho quinasa mediada por arni para el tratamiento de trastornos oculares
US8673873B1 (en) * 2005-12-28 2014-03-18 Alcon Research, Ltd. RNAi-mediated inhibition of phosphodiesterase type 4 for treatment of cAMP-related ocular disorders
AU2007205939B2 (en) * 2006-01-17 2012-12-13 Synthon Biopharmaceuticals B.V. Compositions and methods for humanization and optimization of N-glycans in plants
US20090060921A1 (en) * 2006-01-17 2009-03-05 Biolex Therapeutics, Inc. Glycan-optimized anti-cd20 antibodies
US20120208824A1 (en) 2006-01-20 2012-08-16 Cell Signaling Technology, Inc. ROS Kinase in Lung Cancer
US7825099B2 (en) 2006-01-20 2010-11-02 Quark Pharmaceuticals, Inc. Treatment or prevention of oto-pathologies by inhibition of pro-apoptotic genes
PT2671954T (pt) 2006-01-20 2018-10-08 Cell Signaling Technology Inc Translocação e ros-cinase mutante no carcinoma do pulmão de células não pequenas humano
UY30097A1 (es) 2006-01-20 2007-08-31 Atugen Ag Usos terapeuticos de inhibidores de rtp801
JP2009524419A (ja) * 2006-01-27 2009-07-02 サンタリス ファーマ アー／エス Ｌｎａ修飾したホスホロチオール化オリゴヌクレオチド
US8362229B2 (en) * 2006-02-08 2013-01-29 Quark Pharmaceuticals, Inc. Tandem siRNAS
US7910566B2 (en) 2006-03-09 2011-03-22 Quark Pharmaceuticals Inc. Prevention and treatment of acute renal failure and other kidney diseases by inhibition of p53 by siRNA
FI20060246A0 (fi) 2006-03-16 2006-03-16 Jukka Westermarck Uusi kasvua stimuloiva proteiini ja sen käyttö
US20100056441A1 (en) * 2006-03-17 2010-03-04 Costa Robert H Method for Inhibiting Angiogenesis
EP2002004B1 (en) 2006-03-23 2015-10-14 Roche Innovation Center Copenhagen A/S Small internally segmented interfering rna
FR2898908A1 (fr) 2006-03-24 2007-09-28 Agronomique Inst Nat Rech Procede de preparation de cellules aviaires differenciees et genes impliques dans le maintien de la pluripotence
EP2007435B1 (en) 2006-03-31 2019-12-18 Massachusetts Institute Of Technology System for targeted delivery of therapeutic agents
US9044461B2 (en) 2006-04-07 2015-06-02 The Research Foundation Of State University Of New York Transcobalamin receptor polypeptides, nucleic acids, and modulators thereof, and related methods of use in modulating cell growth and treating cancer and cobalamin deficiency
CA2648581A1 (en) * 2006-04-07 2008-09-12 Chimeros, Inc. Compositions and methods for treating b- cell malignancies
CA2648718A1 (en) 2006-04-07 2007-10-18 The Research Foundation Of State University Of New York Transcobalamin receptor polypeptides, nucleic acids, and modulators thereof, and related methods of use in modulating cell growth and treating cancer and cobalamin deficiency
EP2010225A4 (en) * 2006-04-13 2010-04-21 Cornell Res Foundation Inc METHODS AND COMPOSITIONS FOR TARGETING C-REL
EP2447359B1 (en) 2006-04-14 2015-11-04 Cell Signaling Technology, Inc. Gene defects and mutant ALK kinase in human solid tumors
CA2650140A1 (en) 2006-04-14 2007-10-25 Mriganka Sur Identifying and modulating molecular pathways that mediate nervous system plasticity
CA2652280C (en) 2006-05-15 2014-01-28 Massachusetts Institute Of Technology Polymers for functional particles
EP2026843A4 (en) 2006-06-09 2011-06-22 Quark Pharmaceuticals Inc THERAPEUTIC USES OF RTP801L INHIBITORS
WO2007150030A2 (en) 2006-06-23 2007-12-27 Massachusetts Institute Of Technology Microfluidic synthesis of organic nanoparticles
GB0613753D0 (en) * 2006-07-11 2006-08-23 Norwegian Radium Hospital Res Method
CA2659392A1 (en) 2006-07-13 2008-01-17 University Of Iowa Research Foundation Methods and reagents for treatment and diagnosis of vascular disorders and age-related macular degeneration
WO2008009477A2 (en) * 2006-07-21 2008-01-24 Silence Therapeutics Ag Means for inhibiting the expression of protein kinase 3
DE102006039479A1 (de) 2006-08-23 2008-03-06 Febit Biotech Gmbh Programmierbare Oligonukleotidsynthese
AU2007299748A1 (en) * 2006-09-19 2008-03-27 Asuragen, Inc. miR-15, miR-26, miR -31,miR -145, miR-147, miR-188, miR-215, miR-216 miR-331, mmu-miR-292-3p regulated genes and pathways as targets for therapeutic intervention
CA2663878A1 (en) * 2006-09-19 2008-03-27 Asuragen, Inc. Mir-200 regulated genes and pathways as targets for therapeutic intervention
JP5352462B2 (ja) 2006-09-22 2013-11-27 ダーマコン， インコーポレイテッド 二本鎖オリゴヌクレオチド複合体、ｒｎａ干渉による遺伝子サイレンシング方法、および医薬品組成物
JP2010507387A (ja) 2006-10-25 2010-03-11 クアーク・ファーマスーティカルス、インコーポレイテッド 新規のｓｉＲＮＡおよびその使用方法
EP2407558A1 (en) 2006-10-31 2012-01-18 Noxxon Pharma AG Methods for the detection of a single- or double-stranded nucleic acid molecule
WO2008053487A2 (en) 2006-11-01 2008-05-08 The Medical Research Fund At The Tel-Aviv Sourasky Medical Center Adipocyte-specific constructs and methods for inhibiting platelet-type 12 lipoxygenase expression
US8252526B2 (en) 2006-11-09 2012-08-28 Gradalis, Inc. ShRNA molecules and methods of use thereof
US8758998B2 (en) 2006-11-09 2014-06-24 Gradalis, Inc. Construction of bifunctional short hairpin RNA
US8906874B2 (en) 2006-11-09 2014-12-09 Gradalis, Inc. Bi-functional shRNA targeting Stathmin 1 and uses thereof
JP5271715B2 (ja) * 2006-11-22 2013-08-21 国立大学法人 東京大学 ジスルフィド架橋高分子ミセルを用いた環境応答性ｓｉＲＮＡキャリア
ES2476798T3 (es) 2006-11-27 2014-07-15 Patrys Limited Nueva diana de p�ptido glucosilado en células neopl�sicas
CA2670696A1 (en) 2006-11-27 2008-06-05 Diadexus, Inc. Ovr110 antibody compositions and methods of use
US20090048195A1 (en) * 2006-11-30 2009-02-19 University Of Southern California Compositions and methods of sphingosine kinase inhibitors for use thereof in cancer therapy
EP2104735A2 (en) * 2006-12-08 2009-09-30 Asuragen, INC. Mir-21 regulated genes and pathways as targets for therapeutic intervention
CN101563458A (zh) * 2006-12-14 2009-10-21 诺瓦提斯公司 治疗肌肉和心血管病症的组合物和方法
CA2671270A1 (en) * 2006-12-29 2008-07-17 Asuragen, Inc. Mir-16 regulated genes and pathways as targets for therapeutic intervention
US9896511B2 (en) 2007-01-10 2018-02-20 The United States Of America, As Represented By The Secretary, Dept. Of Health And Human Services Antibodies that bind to TL1A and methods of treating inflammatory or autoimmune disease comprising administering such antibodies
EP2121717A4 (en) * 2007-01-17 2013-05-29 Inst Rech S Cliniques De Montreal NUCLEOSIDIC AND NUCLEOTIDE ANALOGS WITH QUATERNARY CARBON CENTERS AND METHODS OF USE THEREOF
BRPI0806436A2 (pt) 2007-01-26 2011-09-06 Univ Lousville Res Foundation Inc modificação de componentes exossomais para uso como uma vacina
WO2008098165A2 (en) 2007-02-09 2008-08-14 Massachusetts Institute Of Technology Oscillating cell culture bioreactor
WO2008098248A2 (en) 2007-02-09 2008-08-14 Northwestern University Particles for detecting intracellular targets
DE102007008596B4 (de) * 2007-02-15 2010-09-02 Friedrich-Schiller-Universität Jena Biologisch wirksame Moleküle auf Grundlage von PNA und siRNA, Verfahren zu deren zellspezifischen Aktivierung sowie Applikationskit zur Verabreichung
AU2008218813B2 (en) * 2007-02-20 2014-04-17 Monsanto Technology, Llc Invertebrate microRNAs
JP2010518880A (ja) 2007-02-26 2010-06-03 クアーク・ファーマスーティカルス、インコーポレイテッド Ｒｔｐ８０１のインヒビター及びその疾患の治療における使用
WO2008104978A2 (en) * 2007-02-28 2008-09-04 Quark Pharmaceuticals, Inc. Novel sirna structures
US20100055784A1 (en) * 2007-03-02 2010-03-04 Mdrna, Inc. Nucleic acid compounds for inhibiting wnt gene expression and uses thereof
US9018163B2 (en) * 2007-03-02 2015-04-28 The Trustees Of The University Of Pennsylvania Modulating PDX-1 with PCIF1, methods and uses thereof
CA2681323A1 (en) 2007-03-21 2008-09-25 Brookhaven Science Associates, Llc Combined hairpin-antisense compositions and methods for modulating expression
US7812002B2 (en) 2007-03-21 2010-10-12 Quark Pharmaceuticals, Inc. Oligoribonucleotide inhibitors of NRF2 and methods of use thereof for treatment of cancer
WO2008124632A1 (en) 2007-04-04 2008-10-16 Massachusetts Institute Of Technology Amphiphilic compound assisted nanoparticles for targeted delivery
WO2008124634A1 (en) 2007-04-04 2008-10-16 Massachusetts Institute Of Technology Polymer-encapsulated reverse micelles
US20090226525A1 (en) * 2007-04-09 2009-09-10 Chimeros Inc. Self-assembling nanoparticle drug delivery system
WO2008122314A1 (de) 2007-04-10 2008-10-16 Qiagen Gmbh Rna-interferenz tags
AU2008242842B2 (en) * 2007-04-17 2014-06-05 Baxter Healthcare Sa Nucleic acid microparticles for pulmonary delivery
EP2155860B1 (en) 2007-05-03 2014-08-27 The Brigham and Women's Hospital, Inc. Multipotent stem cells and uses thereof
JP5296328B2 (ja) * 2007-05-09 2013-09-25 独立行政法人理化学研究所 １本鎖環状ｒｎａおよびその製造方法
KR20100017422A (ko) * 2007-05-15 2010-02-16 헬리콘 테라퓨틱스 인코퍼레이티드 작은 간섭 ＲＮＡ(ｓｉＲＮＡ)를 이용하여 기억 형성에 관여하는 유전자를 확인하는 방법
US20090131354A1 (en) * 2007-05-22 2009-05-21 Bader Andreas G miR-126 REGULATED GENES AND PATHWAYS AS TARGETS FOR THERAPEUTIC INTERVENTION
EP2160464B1 (en) 2007-05-30 2014-05-21 Northwestern University Nucleic acid functionalized nanoparticles for therapeutic applications
EP2581081A3 (en) 2007-06-01 2013-07-31 The Trustees Of Princeton University Treatment of viral infections by modulation of host cell metabolic pathways
EP3031916B1 (en) * 2007-06-11 2017-06-07 Takara Bio Inc. Method for expression of specific gene
US20100273854A1 (en) * 2007-06-15 2010-10-28 Hagar Kalinski Compositions and methods for inhibiting nadph oxidase expression
EP2170403B1 (en) 2007-06-27 2014-04-16 Quark Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of pro-apoptotic genes
EP2319926B1 (en) * 2007-07-05 2016-08-31 Arrowhead Research Corporation DSRNA for treating viral infection
US8828960B2 (en) * 2007-07-17 2014-09-09 Idexx Laboratories, Inc. Amino acid vitamin ester compositions for controlled delivery of pharmaceutically active compounds
EP2030615A3 (en) 2007-08-13 2009-12-02 ELFORD, Howard L. Ribonucleotide reductase inhibitors for use in the treatment or prevention of neuroinflammatory or autoimmune diseases
CN101842381A (zh) * 2007-08-27 2010-09-22 波士顿生物医药公司 作为微小rna模拟物或抑制剂的不对称rna双链体的组合物
AU2008291604B2 (en) * 2007-08-30 2013-12-05 Akshaya Bio Inc. Antigenic compositions and use of same in the targeted delivery of nucleic acids
WO2009033027A2 (en) 2007-09-05 2009-03-12 Medtronic, Inc. Suppression of scn9a gene expression and/or function for the treatment of pain
JP2010539245A (ja) * 2007-09-14 2010-12-16 日東電工株式会社 薬物担体
CN101849008A (zh) 2007-09-19 2010-09-29 应用生物系统有限公司 用于减少RNAi中的脱靶表型效应的siRNA的不依赖于序列的修饰形式和其稳定形式
EP2197454A4 (en) * 2007-09-25 2012-07-04 Idexx Lab Inc PHARMACEUTICAL COMPOSITIONS FOR THE ADMINISTRATION OF OLIGONUCLEOTIDES
PT2644594T (pt) 2007-09-28 2017-11-20 Pfizer Direcionamento a células cancerosas utilizando nanopartículas
US20120082659A1 (en) * 2007-10-02 2012-04-05 Hartmut Land Methods And Compositions Related To Synergistic Responses To Oncogenic Mutations
BRPI0817605A2 (pt) 2007-10-03 2017-05-09 Quark Pharmaceuticals Inc novas estruturas de sirna
JP2011500569A (ja) 2007-10-12 2011-01-06 マサチューセッツ インスティテュート オブ テクノロジー ワクチンナノテクノロジー
DK2203558T3 (da) 2007-10-18 2016-06-27 Cell Signaling Technology Inc Translokation og muteret ros-kinase i humant ikke-småcellet lungekarcinom
EP2268664B1 (en) 2007-12-03 2017-05-24 The Government of the United States of America as represented by the Secretary of the Department of Health and Human Services Doc1 compositions and methods for treating cancer
WO2009074990A2 (en) * 2007-12-12 2009-06-18 Quark Pharmaceuticals, Inc. Rtp801l sirna compounds and methods of use thereof
US8614311B2 (en) 2007-12-12 2013-12-24 Quark Pharmaceuticals, Inc. RTP801L siRNA compounds and methods of use thereof
US7845686B2 (en) * 2007-12-17 2010-12-07 S & B Technical Products, Inc. Restrained pipe joining system for plastic pipe
KR100949791B1 (ko) * 2007-12-18 2010-03-30 이동기 오프-타겟 효과를 최소화하고 ＲＮＡｉ 기구를 포화시키지않는 신규한 ｓｉＲＮＡ 구조 및 그 용도
US20090192114A1 (en) * 2007-12-21 2009-07-30 Dmitriy Ovcharenko miR-10 Regulated Genes and Pathways as Targets for Therapeutic Intervention
WO2009090639A2 (en) * 2008-01-15 2009-07-23 Quark Pharmaceuticals, Inc. Sirna compounds and methods of use thereof
EP2260110B1 (en) * 2008-02-08 2014-11-12 Asuragen, INC. miRNAs DIFFERENTIALLY EXPRESSED IN LYMPH NODES FROM CANCER PATIENTS
US8188060B2 (en) 2008-02-11 2012-05-29 Dharmacon, Inc. Duplex oligonucleotides with enhanced functionality in gene regulation
DE102009043743B4 (de) 2009-03-13 2016-10-13 Friedrich-Schiller-Universität Jena Zellspezifisch wirksame Moleküle auf Grundlage von siRNA sowie Applikationskits zu deren Herstellung und Verwendung
WO2009103067A2 (en) * 2008-02-14 2009-08-20 The Children's Hospital Of Philadelphia Compositions and methods to treat asthma
EP2268832A2 (en) * 2008-03-06 2011-01-05 Asuragen, INC. Microrna markers for recurrence of colorectal cancer
CN102036689B (zh) * 2008-03-17 2014-08-06 得克萨斯系统大学董事会 神经肌肉突触维持和再生中涉及的微小rna的鉴定
AU2009227549A1 (en) * 2008-03-20 2009-09-24 Quark Pharmaceuticals, Inc. Novel siRNA compounds for inhibiting RTP801
WO2009154835A2 (en) * 2008-03-26 2009-12-23 Asuragen, Inc. Compositions and methods related to mir-16 and therapy of prostate cancer
KR20100131509A (ko) * 2008-03-31 2010-12-15 내셔날 인스티튜트 오브 어드밴스드 인더스트리얼 사이언스 앤드 테크놀로지 Ｒｎａ 간섭 효과가 높은 2본쇄 지질 수식 ｒｎａ
WO2009126726A1 (en) * 2008-04-08 2009-10-15 Asuragen, Inc Methods and compositions for diagnosing and modulating human papillomavirus (hpv)
US8309614B2 (en) * 2008-04-11 2012-11-13 Cedars-Sinai Medical Center Poly(beta malic acid) with pendant leu-leu-leu tripeptide for effective cytoplasmic drug delivery
WO2009126933A2 (en) 2008-04-11 2009-10-15 Alnylam Pharmaceuticals, Inc. Site-specific delivery of nucleic acids by combining targeting ligands with endosomolytic components
WO2009144704A2 (en) * 2008-04-15 2009-12-03 Quark Pharmaceuticals, Inc. siRNA COMPOUNDS FOR INHIBITING NRF2
KR20170005148A (ko) 2008-04-21 2017-01-11 티슈 리제너레이션 쎄라퓨틱스, 인코포레이티드 생물학적 또는 화학적 물질에 대한 예방 또는 치료를 위한 유전적으로 변형된 인간 탯줄 혈관주위 세포
WO2009134443A2 (en) * 2008-05-02 2009-11-05 The Brigham And Women's Hospital, Inc. Rna-induced translational silencing and cellular apoptosis
US20090291073A1 (en) * 2008-05-20 2009-11-26 Ward Keith W Compositions Comprising PKC-theta and Methods for Treating or Controlling Ophthalmic Disorders Using Same
WO2009146417A1 (en) * 2008-05-30 2009-12-03 Sigma-Aldrich Co. Compositions and methods for specifically silencing a target nucleic acid
US20090305611A1 (en) * 2008-06-06 2009-12-10 Flow International Corporation Device and method for improving accuracy of a high-pressure fluid jet apparatus
WO2010005741A1 (en) * 2008-06-16 2010-01-14 Georgia Tech Research Corporation Nanogels for cellular delivery of therapeutics
TWI455944B (zh) 2008-07-01 2014-10-11 Daiichi Sankyo Co Ltd 雙股多核苷酸
US8309791B2 (en) 2008-07-16 2012-11-13 Recombinectics, Inc. Method for producing a transgenic pig using a hyper-methylated transposon
US8039658B2 (en) * 2008-07-25 2011-10-18 Air Products And Chemicals, Inc. Removal of trace arsenic impurities from triethylphosphate (TEPO)
US8212019B2 (en) * 2008-07-30 2012-07-03 University Of Massachusetts Nucleic acid silencing sequences
WO2011028218A1 (en) 2009-09-02 2011-03-10 Alnylam Pharmaceuticals, Inc. Process for triphosphate oligonucleotide synthesis
WO2010039548A2 (en) 2008-09-23 2010-04-08 Alnylam Pharmaceuticals, Inc. Chemical modifications of monomers and oligonucleotides with cycloaddition
US9388413B2 (en) 2008-10-08 2016-07-12 Trustees Of Dartmouth College Method for selectively inhibiting ACAT1 in the treatment of neurodegenerative diseases
US8802646B2 (en) * 2008-10-08 2014-08-12 Trustees Of Dartmouth College Method for selectively inhibiting the activity of ACAT1 in the treatment of alzheimer's disease
US9388414B2 (en) 2008-10-08 2016-07-12 Trustees Of Dartmouth College Method for selectively inhibiting ACAT1 in the treatment of neurodegenerative diseases
US9149492B2 (en) 2008-10-08 2015-10-06 Trustees Of Dartmouth College Method for selectively inhibiting ACAT1 in the treatment of alzheimer's disease
WO2010042292A1 (en) * 2008-10-08 2010-04-15 Trustees Of Dartmouth College Method for selectively inhibiting the activity of acat1 in the treatment of alzheimer's disease
US8343498B2 (en) 2008-10-12 2013-01-01 Massachusetts Institute Of Technology Adjuvant incorporation in immunonanotherapeutics
US8591905B2 (en) 2008-10-12 2013-11-26 The Brigham And Women's Hospital, Inc. Nicotine immunonanotherapeutics
US8343497B2 (en) 2008-10-12 2013-01-01 The Brigham And Women's Hospital, Inc. Targeting of antigen presenting cells with immunonanotherapeutics
US8277812B2 (en) 2008-10-12 2012-10-02 Massachusetts Institute Of Technology Immunonanotherapeutics that provide IgG humoral response without T-cell antigen
US9458472B2 (en) * 2008-10-15 2016-10-04 Massachusetts Institute Of Technology Detection and destruction of cancer cells using programmed genetic vectors
EP3238738B1 (en) 2008-11-10 2020-09-23 Arbutus Biopharma Corporation Novel lipids and compositions for the delivery of therapeutics
US20100179213A1 (en) * 2008-11-11 2010-07-15 Mirna Therapeutics, Inc. Methods and Compositions Involving miRNAs In Cancer Stem Cells
CA2742771A1 (en) * 2008-11-13 2010-05-20 Modgene, Llc Modification of amyloid-beta load in non-brain tissue
JP2012509331A (ja) 2008-11-21 2012-04-19 アイシス ファーマシューティカルズ， インコーポレーテッド がんの治療のための併用療法
EP3335705A1 (en) 2008-11-24 2018-06-20 Northwestern University Polyvalent rna-nanoparticle compositions
EP2191834A1 (en) * 2008-11-26 2010-06-02 Centre National De La Recherche Scientifique (Cnrs) Compositions and methods for treating retrovirus infections
KR20110097915A (ko) 2008-12-03 2011-08-31 마리나 바이오테크, 인크. ＵｓｉＲＮＡ 복합체
PE20120341A1 (es) 2008-12-09 2012-04-24 Genentech Inc Anticuerpos anti-pd-l1 y su uso para mejorar la funcion de celulas t
US20100233172A1 (en) * 2008-12-16 2010-09-16 Bristol-Myers Squibb Company Methods of inhibiting quiescent tumor proliferation
US20110288155A1 (en) 2008-12-18 2011-11-24 Elena Feinstein Sirna compounds and methods of use thereof
CA2748520C (en) 2008-12-26 2013-12-17 Se-Ho Kim Pharmaceutical composition containing an anionic drug, and a production method therefor
US20100233270A1 (en) 2009-01-08 2010-09-16 Northwestern University Delivery of Oligonucleotide-Functionalized Nanoparticles
WO2010083532A1 (en) * 2009-01-19 2010-07-22 The Research Foundaton Of State University Of New York Fatty acid binding proteins as drug targets for modulation of endocannabinoids
AU2010206143A1 (en) * 2009-01-20 2011-08-25 Life Sciences Research Partners Vzw PHD2 inhibition for blood vessel normalization, and uses thereof
CA2744236C (en) 2009-02-12 2021-03-16 Cell Signaling Technology, Inc. Mutant ros expression in human cancer
CN102333869A (zh) * 2009-02-24 2012-01-25 里博克斯艾克斯有限公司 小干扰rna的改进设计
EP2669290A1 (en) 2009-03-02 2013-12-04 Alnylam Pharmaceuticals Inc. Nucleic Acid Chemical Modifications
CN102439151A (zh) * 2009-03-19 2012-05-02 默沙东公司 使用短干扰核酸（siNA）的RNA干扰介导的BTB和CNC同系物1，碱性亮氨酸拉链转录因子1（Bach1）基因表达的抑制
WO2010120874A2 (en) 2009-04-14 2010-10-21 Chimeros, Inc. Chimeric therapeutics, compositions, and methods for using same
US8367350B2 (en) 2009-04-29 2013-02-05 Morehouse School Of Medicine Compositions and methods for diagnosis, prognosis and management of malaria
US9255266B2 (en) 2009-05-06 2016-02-09 Rutgers, The State University Of New Jersey RNA targeting in alpha-synucleinopathies
US20120114710A1 (en) * 2009-05-18 2012-05-10 Lynn Kirkpatrick Carbon nanotubes complexed with multiple bioactive agents and methods related thereto
US20120128673A1 (en) 2009-05-20 2012-05-24 Schering Corporation Modulation of pilr receptors to treat microbial infections
US20100304995A1 (en) * 2009-05-22 2010-12-02 Li Shen Arrays and Methods for Reverse Genetic Functional Analysis
US20120083519A1 (en) * 2009-06-03 2012-04-05 Djillali Sahali Methods For Diagnosing And Treating A Renal Disease In An Individual
CA2764503A1 (en) 2009-06-05 2010-12-09 University Of Florida Research Foundation, Inc. Isolation and targeted suppression of lignin biosynthetic genes from sugarcane
US8637482B2 (en) 2009-06-08 2014-01-28 Quark Pharmaceuticals, Inc. Methods for treating chronic kidney disease
EP2440666B1 (en) 2009-06-10 2017-03-01 Temasek Life Sciences Laboratory Limited Virus induced gene silencing (vigs) for functional analysis of genes in cotton
SI3431076T1 (sl) 2009-06-10 2022-04-29 Arbutus Biopharma Corporation Izboljšana lipidna formulacija
US20100324124A1 (en) * 2009-06-17 2010-12-23 Massachusetts Institute Of Technology Compositions and methods relating to DNA-based particles
US20100323018A1 (en) * 2009-06-17 2010-12-23 Massachusetts Institute Of Technology Branched DNA/RNA monomers and uses thereof
GB0910723D0 (en) 2009-06-22 2009-08-05 Sylentis Sau Novel drugs for inhibition of gene expression
DK2769737T3 (da) 2009-07-20 2017-07-24 Bristol Myers Squibb Co Kombination af et anti-ctla4-antistof med etoposid til synergistisk behandling af proliferative sygdomme
WO2011020024A2 (en) 2009-08-13 2011-02-17 The Johns Hopkins University Methods of modulating immune function
CN102481379A (zh) 2009-08-24 2012-05-30 菲吉尼克斯公司 靶向pax2用于治疗乳腺癌
WO2011031600A1 (en) 2009-09-10 2011-03-17 Schering Corporation Use of il-33 antagonists to treat fibrotic disease
WO2011032100A1 (en) 2009-09-11 2011-03-17 Government Of The U.S.A., As Represented By The Secretary, Department Of Health And Human Services Inhibitors of kshv vil6 and human il6
EP2295543A1 (en) 2009-09-11 2011-03-16 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Method for the preparation of an influenza virus
US20150025122A1 (en) 2009-10-12 2015-01-22 Larry J. Smith Methods and Compositions for Modulating Gene Expression Using Oligonucleotide Based Drugs Administered in vivo or in vitro
DK2494075T3 (da) 2009-10-30 2018-07-23 Univ Northwestern Skabelonstyrede nanokonjugater
US8901097B2 (en) 2009-11-08 2014-12-02 Quark Pharmaceuticals, Inc. Methods for delivery of siRNA to the spinal cord and therapies arising therefrom
WO2011062997A2 (en) 2009-11-17 2011-05-26 Musc Foundation For Research Development Human monoclonal antibodies to human nucleolin
KR20120102630A (ko) 2009-11-26 2012-09-18 쿠아크 파마수티칼스 인코퍼레이티드 말단 치환체를 포함하는 에스아이 알엔에이 화합물
EP3296398A1 (en) 2009-12-07 2018-03-21 Arbutus Biopharma Corporation Compositions for nucleic acid delivery
CA2781896C (en) * 2009-12-09 2021-03-30 Nitto Denko Corporation Modulation of hsp47 expression
EP2510098B1 (en) 2009-12-09 2015-02-11 Quark Pharmaceuticals, Inc. Methods and compositions for treating diseases, disorders or injury of the cns
WO2011072240A1 (en) 2009-12-10 2011-06-16 Cedars-Sinai Medical Center Drug delivery of temozolomide for systemic based treatment of cancer
EP2336171A1 (en) 2009-12-11 2011-06-22 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Novel targets for the treatment of proliferative diseases
EP2513308B1 (en) 2009-12-17 2017-01-18 Merck Sharp & Dohme Corp. Modulation of pilr to treat immune disorders
AU2010330814B2 (en) 2009-12-18 2017-01-12 Acuitas Therapeutics Inc. Methods and compositions for delivery of nucleic acids
WO2011073326A2 (en) 2009-12-18 2011-06-23 Novartis Ag Organic compositions to treat hsf1-related diseases
JP2013515697A (ja) 2009-12-23 2013-05-09 マックスプランク−ゲセルシャフト・ツール・フェーデルング・デル・ヴィッセンシャフテン・エー・ファウ インフルエンザ標的
CN102917709B (zh) 2009-12-23 2018-04-24 格兰达利斯有限公司 弗林蛋白酶敲减和gm-csf增强的（fang）癌症疫苗
US20130023578A1 (en) 2009-12-31 2013-01-24 Samyang Biopharmaceuticals Corporation siRNA for inhibition of c-Met expression and anticancer composition containing the same
WO2011084193A1 (en) 2010-01-07 2011-07-14 Quark Pharmaceuticals, Inc. Oligonucleotide compounds comprising non-nucleotide overhangs
EP2524039B1 (en) * 2010-01-11 2017-11-29 CuRNA, Inc. Treatment of sex hormone binding globulin (shbg) related diseases by inhibition of natural antisense transcript to shbg
WO2011088058A1 (en) * 2010-01-12 2011-07-21 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expressions of factor vii and pten genes
DE102010004957A1 (de) 2010-01-14 2011-07-21 Universitätsklinikum Jena, 07743 Biologisch wirksame Moleküle zur Beeinflussung von Virus-, Bakterien-, Parasiten-infizierten Zellen und/oder Tumorzellen und Verfahren zu deren Anwendung
WO2011094580A2 (en) 2010-01-28 2011-08-04 Alnylam Pharmaceuticals, Inc. Chelated copper for use in the preparation of conjugated oligonucleotides
WO2011100131A2 (en) 2010-01-28 2011-08-18 Alnylam Pharmacuticals, Inc. Monomers and oligonucleotides comprising cycloaddition adduct(s)
KR20120125357A (ko) 2010-02-10 2012-11-14 노파르티스 아게 근육 성장을 위한 방법 및 화합물
WO2011123621A2 (en) 2010-04-01 2011-10-06 Alnylam Pharmaceuticals Inc. 2' and 5' modified monomers and oligonucleotides
WO2011133658A1 (en) 2010-04-22 2011-10-27 Boston Medical Center Corporation Compositions and methods for targeting and delivering therapeutics into cells
WO2011133868A2 (en) 2010-04-22 2011-10-27 Alnylam Pharmaceuticals, Inc. Conformationally restricted dinucleotide monomers and oligonucleotides
US9725479B2 (en) 2010-04-22 2017-08-08 Ionis Pharmaceuticals, Inc. 5′-end derivatives
WO2011133876A2 (en) 2010-04-22 2011-10-27 Alnylam Pharmaceuticals, Inc. Oligonucleotides comprising acyclic and abasic nucleosides and analogs
RS56011B1 (sr) 2010-04-29 2017-09-29 Ionis Pharmaceuticals Inc Modulacija ekspresije transtiretina
EP2563359A1 (en) 2010-04-30 2013-03-06 Allergan, Inc. Novel treatment for age related macular degeneration and ocular ischemic disease associated with complement activation by targeting 5-lipoxygenase
ES2596431T3 (es) 2010-05-04 2017-01-09 The Brigham And Women's Hospital, Inc. Antagonista de cadherina-11 para el tratamiento de fibrosis
EA023397B1 (ru) 2010-05-26 2016-05-31 Селекта Байосайенсиз, Инк. Комбинированные вакцины с синтетическими наноносителями
EP2390327A1 (en) 2010-05-27 2011-11-30 Sylentis S.A. siRNA and their use in methods and compositions for the treatment and/or prevention of eye conditions
DE102010022937A1 (de) 2010-06-04 2011-12-08 Universitätsklinikum Jena Zellspezifisch aktivierbare biologisch wirksame Moleküle auf Grundlage von siRNA, Verfahren zu deren Aktivierung sowie Applikationskit zur Verabreichung
WO2011163121A1 (en) 2010-06-21 2011-12-29 Alnylam Pharmaceuticals, Inc. Multifunctional copolymers for nucleic acid delivery
SG186438A1 (en) 2010-06-24 2013-01-30 Quark Pharmaceuticals Inc Double stranded rna compounds to rhoa and use thereof
US20130323269A1 (en) 2010-07-30 2013-12-05 Muthiah Manoharan Methods and compositions for delivery of active agents
US20130202652A1 (en) 2010-07-30 2013-08-08 Alnylam Pharmaceuticals, Inc. Methods and compositions for delivery of active agents
US20120101108A1 (en) 2010-08-06 2012-04-26 Cell Signaling Technology, Inc. Anaplastic Lymphoma Kinase In Kidney Cancer
EP3372684B1 (en) 2010-08-24 2020-10-07 Sirna Therapeutics, Inc. Single-stranded rnai agents containing an internal, non-nucleic acid spacer
WO2012051491A1 (en) 2010-10-14 2012-04-19 The United States Of America, As Represented By The Secretary National Institutes Of Health Compositions and methods for controlling neurotropic viral pathogenesis by micro-rna targeting
KR101328568B1 (ko) * 2010-10-22 2013-11-13 성균관대학교산학협력단 Ｒｎａ 간섭을 유도하는 핵산 분자 및 그 용도
WO2012071436A1 (en) 2010-11-24 2012-05-31 Genentech, Inc. Method of treating autoimmune inflammatory disorders using il-23r loss-of-function mutants
AU2011338682B2 (en) 2010-12-06 2017-04-27 Quark Pharmaceuticals, Inc. Double stranded oligonucleotide compounds comprising threose modifications
WO2012102793A2 (en) 2010-12-10 2012-08-02 Zirus, Inc. Mammalian genes involved in toxicity and infection
US8575328B2 (en) * 2010-12-14 2013-11-05 The United States Of America, As Represented By The Secretary Of Agriculture Formicidae (ant) control using double-stranded RNA constructs
US9623041B2 (en) 2010-12-30 2017-04-18 Cedars-Sinai Medical Center Polymalic acid-based nanobiopolymer compositions
CN105646709A (zh) 2011-01-10 2016-06-08 密执安大学评议会 干细胞因子抑制剂
CA2824526C (en) 2011-01-11 2020-07-07 Alnylam Pharmaceuticals, Inc. Pegylated lipids and their use for drug delivery
DE102011009470A1 (de) 2011-01-21 2012-08-09 Friedrich-Schiller-Universität Jena Biologisch wirksame Nukleotid-Moleküle zur gezielten Abtötung von Zellen, Verwendung derselben sowie Applikationskit
CA2828544A1 (en) * 2011-03-03 2012-09-07 Quark Pharmaceuticals, Inc. Oligonucleotide modulators of the toll-like receptor pathway
US9796979B2 (en) 2011-03-03 2017-10-24 Quark Pharmaceuticals Inc. Oligonucleotide modulators of the toll-like receptor pathway
US20140303013A1 (en) 2011-03-15 2014-10-09 University Of Utah Research Foundation Methods of diagnosing and treating vascular associated maculopathy and symptoms thereof
WO2012135696A2 (en) * 2011-04-01 2012-10-04 University Of South Alabama Methods and compositions for the diagnosis, classification, and treatment of cancer
EP2686342A2 (en) 2011-04-12 2014-01-22 The Government of The United States of America, as represented by The Secretary, Department of Health and Human Services Peptide mimetic ligands of polo-like kinase 1 polo box domain and methods of use
CA2832972C (en) 2011-04-13 2019-04-30 Isis Pharmaceuticals, Inc. Antisense modulation of ptp1b expression
US8716257B2 (en) * 2011-04-15 2014-05-06 Sutter West Bay Hospitals CMV gene products promote cancer stem cell growth
EP2696678B1 (en) * 2011-04-15 2019-11-20 Molecular Transfer, Inc. Agents for improved delivery of nucleic acids to eukaryotic cells
CA2836898A1 (en) 2011-05-20 2012-11-29 Government Of The United States, As Represented By The Secretary, Department Of Health And Human Services Blockade of tl1a-dr3 interactions to ameliorate t cell mediated disease pathology and antibodies thereof
WO2012167173A1 (en) 2011-06-02 2012-12-06 The University Of Louisville Research Foundation, Inc. Anti-nucleolin agent-conjugated nanoparticles
US20140227293A1 (en) 2011-06-30 2014-08-14 Trustees Of Boston University Method for controlling tumor growth, angiogenesis and metastasis using immunoglobulin containing and proline rich receptor-1 (igpr-1)
WO2013001372A2 (en) 2011-06-30 2013-01-03 University Of Oslo Methods and compositions for inhibition of activation of regulatory t cells
EP2729173B1 (en) 2011-07-06 2016-06-15 Sykehuset Sorlandet HF Egfr targeted therapy
WO2013006861A1 (en) 2011-07-07 2013-01-10 University Of Georgia Research Foundation, Inc. Sorghum grain shattering gene and uses thereof in altering seed dispersal
US8853181B2 (en) 2011-07-21 2014-10-07 Albert Einstein College Of Medicine Of Yeshiva University Fidgetin-like 2 as a target to enhance wound healing
US9120858B2 (en) 2011-07-22 2015-09-01 The Research Foundation Of State University Of New York Antibodies to the B12-transcobalamin receptor
EP2753696B1 (en) 2011-09-06 2017-11-22 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. The mirna-212/132 family as a therapeutic target
US8865188B2 (en) 2011-09-09 2014-10-21 Biomed Realty, L.P. Methods and compositions for controlling assembly of viral proteins
US9701623B2 (en) 2011-09-27 2017-07-11 Alnylam Pharmaceuticals, Inc. Di-aliphatic substituted pegylated lipids
US9951349B2 (en) 2011-09-27 2018-04-24 Yale University Compositions and methods for transient expression of recombinant RNA
EP3960726A1 (en) 2011-10-18 2022-03-02 Dicerna Pharmaceuticals, Inc. Amine cationic lipids and uses thereof
EP2776565A1 (en) 2011-11-08 2014-09-17 Quark Pharmaceuticals, Inc. Methods and compositions for treating diseases, disorders or injury of the nervous system
EP2725103A3 (en) 2011-11-14 2016-01-06 Silenseed Ltd Methods and compositions for treating prostate cancer
WO2013082529A1 (en) 2011-12-02 2013-06-06 Yale University Enzymatic synthesis of poly(amine-co-esters) and methods of use thereof for gene delivery
JP2015509085A (ja) 2012-01-01 2015-03-26 キュービーアイ エンタープライゼズ リミテッドＱｂｉ Ｅｎｔｅｒｐｒｉｓｅｓ Ｌｔｄ． 治療剤および診断剤の選択的送達のためのｅｎｄｏ１８０を標的とする粒子
US10023862B2 (en) 2012-01-09 2018-07-17 Arrowhead Pharmaceuticals, Inc. Organic compositions to treat beta-catenin-related diseases
CN104080912A (zh) 2012-01-12 2014-10-01 夸克制药公司 用于治疗听力和平衡障碍的组合疗法
US20150126438A1 (en) 2012-01-24 2015-05-07 Beth Israel Deaconess Medical Center, Inc. Novel ChREBP Isoforms and Methods Using the Same
EP2825209B1 (en) 2012-03-14 2018-08-29 University of Central Florida Research Foundation, Inc. Neurofibromatoses therapeutic agents and screening for same
SI2838998T1 (en) 2012-04-18 2018-04-30 Cell Signaling Technology, Inc. EGFR AND ROS1 PRI CRAK
US20150299696A1 (en) 2012-05-02 2015-10-22 Sirna Therapeutics, Inc. SHORT INTERFERING NUCLEIC ACID (siNA) COMPOSITIONS
WO2013166043A1 (en) 2012-05-02 2013-11-07 Children's Hospital Medical Center Rejuvenation of precursor cells
EP2853597B1 (en) 2012-05-22 2018-12-26 Olix Pharmaceuticals, Inc. Rna-interference-inducing nucleic acid molecule able to penetrate into cells, and use therefor
DK2867368T3 (da) 2012-07-06 2022-01-31 Roussy Inst Gustave Samtidig detektering af kannibalisme og senescens som prognostisk markør for cancer
WO2014018375A1 (en) 2012-07-23 2014-01-30 Xenon Pharmaceuticals Inc. Cyp8b1 and uses thereof in therapeutic and diagnostic methods
WO2014036427A1 (en) * 2012-08-31 2014-03-06 The General Hospital Corporation Biotin complexes for treatment and diagnosis of alzheimer's disease
US9932578B2 (en) 2012-09-12 2018-04-03 Quark Pharmaceuticals, Inc. Double-stranded oligonucleotide molecules to P53 and methods of use thereof
DK2895608T3 (da) 2012-09-12 2019-01-21 Quark Pharmaceuticals Inc Dobbeltstrengede oligonukleotidmolekyler til p53 og fremgangsmåder til anvendelse deraf
AU2013318338B2 (en) 2012-09-21 2017-05-25 Intensity Therapeutics, Inc Method of treating cancer
WO2014055825A1 (en) 2012-10-04 2014-04-10 The United States Of America, As Represented By The Secretary, Department Of Health & Human Services A formulation of mycobacterial components as an adjuvant for inducing th17 responses
WO2014068072A1 (en) 2012-10-31 2014-05-08 Institut Gustave-Roussy Identification, assessment and therapy of essential thrombocythemia with resistance to jak2 inhibitors
CA2930339A1 (en) * 2012-11-13 2014-05-22 Jan Lotvall Delivery of therapeutic agent
DE102012022596B4 (de) 2012-11-15 2017-05-04 Friedrich-Schiller-Universität Jena Neue zellspezifisch wirksame Nukleotid-Moleküle und Applikationskit zu deren Anwendung
CA2891673A1 (en) 2012-11-15 2014-05-22 Apellis Pharmaceuticals, Inc. Cell-reactive, long-acting, or targeted compstatin analogs and related compositions and methods
WO2014093688A1 (en) 2012-12-12 2014-06-19 1Massachusetts Institute Of Technology Compositions and methods for functional nucleic acid delivery
EA037709B1 (ru) 2012-12-21 2021-05-13 Сюкехюсе Сёрланне Хф Способ лечения невропатической боли
US10258682B2 (en) 2013-01-16 2019-04-16 The United States Of America, As Represented By The Secretary, Department Of Health & Human Services Attenuated chlamydia vaccine
DE102013003869B4 (de) 2013-02-27 2016-11-24 Friedrich-Schiller-Universität Jena Verfahren zur gezielten Abtötung von Zellen durch zur mRNA-Anbindung ausgerichtete Nukleotid-Moleküle sowie Nukleotid-Moleküle und Applikationskit für solche Verwendung
KR102310921B1 (ko) 2013-03-14 2021-10-13 다이서나 파마수이티컬, 인크. 음이온성 약제를 제형화하는 방법
WO2014152391A1 (en) 2013-03-15 2014-09-25 Apellis Pharmaceuticals, Inc. Cell-penetrating compstatin analogs and uses thereof
CA2918194C (en) 2013-03-27 2022-12-06 The General Hospital Corporation Methods and agents for treating alzheimer's disease
EP2997373A1 (en) 2013-05-17 2016-03-23 MedImmune, LLC Receptors for b7-h4
AU2014302038B2 (en) 2013-06-25 2019-11-14 Epiaxis Therapeutics Pty Ltd Methods and compositions for modulating cancer stem cells
TW201534578A (zh) 2013-07-08 2015-09-16 Daiichi Sankyo Co Ltd 新穎脂質
WO2015006554A1 (en) 2013-07-10 2015-01-15 The Regents Of The University Of Michigan Therapeutic antibodies and uses thereof
US10711106B2 (en) 2013-07-25 2020-07-14 The University Of Chicago High aspect ratio nanofibril materials
WO2015015496A1 (en) 2013-07-31 2015-02-05 Qbi Enterprises Ltd. Sphingolipid-polyalkylamine-oligonucleotide compounds
EP3027223A1 (en) 2013-07-31 2016-06-08 QBI Enterprises Ltd. Methods of use of sphingolipid polyalkylamine oligonucleotide compounds
EP3043774B1 (en) 2013-09-11 2020-11-04 Eagle Biologics, Inc. Liquid protein formulations containing ionic liquids
AU2014324092B2 (en) 2013-09-18 2020-02-06 Epiaxis Therapeutics Pty Ltd Stem cell modulation II
CA2925129C (en) 2013-10-04 2023-04-04 Novartis Ag 3' end caps for rnai agents for use in rna interference
WO2015050871A2 (en) 2013-10-04 2015-04-09 Novartis Ag Organic compounds to treat hepatitis b virus
EP2865756A1 (en) 2013-10-22 2015-04-29 Sylentis, S.A.U. siRNA and their use in methods and compositions for inhibiting the expression of the FLAP gene.
EP2865758A1 (en) 2013-10-22 2015-04-29 Sylentis, S.A.U. siRNA and their use in methods and compositions for inhibiting the expression of the ORAI1 gene
EP2865757A1 (en) 2013-10-22 2015-04-29 Sylentis, S.A.U. siRNA and their use in methods and compositions for inhibiting the expression of the PDK1 gene.
US10004814B2 (en) 2013-11-11 2018-06-26 Sirna Therapeutics, Inc. Systemic delivery of myostatin short interfering nucleic acids (siNA) conjugated to a lipophilic moiety
CN105960410B (zh) 2013-11-21 2020-12-04 硒瑞恩生物科技有限公司 硒衍生的核酸复合物的结构测定方法
EP3077511A4 (en) 2013-12-06 2017-07-05 Dicerna Pharmaceuticals Inc. Methods and compositions for the specific inhibition of transthyretin (ttr) by double-stranded rna
CN104830906B (zh) 2014-02-12 2018-09-04 北京维通达生物技术有限公司 一种重编程获得功能性人肝脏实质细胞的方法
ES2782125T3 (es) 2014-03-11 2020-09-10 Cellectis Método para generar linfocitos T compatibles para trasplante alogénico
DK3119887T3 (da) * 2014-03-20 2019-05-20 Oommen Varghese Forbedrede korte interfererende ribonukleinsyremolekyler
SG11201608109TA (en) 2014-04-01 2016-10-28 Ionis Pharmaceuticals Inc Compositions for modulating sod-1 expression
CN106456771B9 (zh) 2014-05-02 2021-07-20 国家儿童医院研究所 用于抗lyst免疫调节的组合物和方法
US9937270B2 (en) 2014-05-12 2018-04-10 The John Hopkins University Engineering synthethic brain penetrating gene vectors
WO2015175545A1 (en) 2014-05-12 2015-11-19 The Johns Hopkins University Highly stable biodegradable gene vector platforms for overcoming biological barriers
WO2015184105A1 (en) 2014-05-29 2015-12-03 Trustees Of Dartmouth College Method for selectively inhibiting acat1 in the treatment of neurodegenerative diseases
TR201908550T4 (tr) 2014-06-04 2019-07-22 Exicure Inc Profilaktik veya terapötik uygulamalar için lipozomal sferik nükleik asitler tarafından immün modülatörlerin çok değerlikli teslimi.
CN104120127B (zh) * 2014-07-01 2016-09-21 清华大学 分离的寡核苷酸及其应用
US20170348402A1 (en) 2014-07-30 2017-12-07 The Research Foundation For The State University Of New York System and method for delivering genetic material or protein to cells
EP3180033B1 (de) 2014-08-14 2023-06-07 Friedrich-Schiller-Universität Jena Peptid zur verwendung in der reduktion von nebenwirkungen in form von immunstimulatorischen reaktionen/effekten
CA2958704A1 (en) 2014-08-25 2016-03-03 University Of Canberra Compositions for modulating cancer stem cells and uses therefor
JP6841753B2 (ja) 2014-09-15 2021-03-10 ザ チルドレンズ メディカル センター コーポレーション ヒストンｈ３−リジントリメチル化を除去することによって体細胞核移入（ｓｃｎｔ）効率を増加させるための方法および組成物
JP2017531629A (ja) 2014-09-25 2017-10-26 コールド スプリング ハーバー ラボラトリー レット症候群の処置
EP3200804A4 (en) 2014-10-01 2018-04-18 Eagle Biologics, Inc. Polysaccharide and nucleic acid formulations containing viscosity-lowering agents
US20170304459A1 (en) 2014-10-10 2017-10-26 Alnylam Pharmaceuticals, Inc. Methods and compositions for inhalation delivery of conjugated oligonucleotide
KR20170072244A (ko) 2014-10-10 2017-06-26 이데라 파마슈티칼즈, 인코포레이티드 관문 억제제를 가지는 tlr9 효능제를 이용한 암 치료
US10538762B2 (en) 2014-10-14 2020-01-21 The Board Of Regents Of The University Of Texas System Allele selective inhibition of mutant C9orf72 foci expression by duplex RNAS targeting the expanded hexanucleotide repeat
AU2015336954A1 (en) 2014-10-22 2017-06-08 Katholieke Universiteit Leuven Ku Leuven Research & Development Modulating adipose tissue and adipogenesis
WO2016077624A1 (en) 2014-11-12 2016-05-19 Nmc, Inc. Transgenic plants with engineered redox sensitive modulation of photosynthetic antenna complex pigments and methods for making the same
WO2016081621A1 (en) 2014-11-18 2016-05-26 Yale University Formulations for targeted release of agents under low ph conditions and methods of use thereof
US11213593B2 (en) 2014-11-21 2022-01-04 Northwestern University Sequence-specific cellular uptake of spherical nucleic acid nanoparticle conjugates
WO2016094374A1 (en) * 2014-12-09 2016-06-16 The Board Of Regents Of The University Of Texas System Compositions and mentods for treatment of friedreich's ataxia
US10264976B2 (en) 2014-12-26 2019-04-23 The University Of Akron Biocompatible flavonoid compounds for organelle and cell imaging
US20180002702A1 (en) * 2014-12-26 2018-01-04 Nitto Denko Corporation Methods and compositions for treating malignant tumors associated with kras mutation
US10792299B2 (en) 2014-12-26 2020-10-06 Nitto Denko Corporation Methods and compositions for treating malignant tumors associated with kras mutation
US20180126014A1 (en) 2015-04-15 2018-05-10 Yale University Compositions for enhancing delivery of agents across the blood brain barrier and methods of use thereof
EP3291839A1 (en) 2015-05-05 2018-03-14 The University of Louisville Research Foundation, Inc. Anti-nucleolin agent-conjugated nanoparticles as radio-sensitizers and mri and/or x-ray contrast agents
EP3302710A4 (en) 2015-06-03 2019-02-20 The University of Queensland MOBILIZERS AND USE THEREOF
JP2018520125A (ja) 2015-06-10 2018-07-26 ボード・オブ・リージエンツ，ザ・ユニバーシテイ・オブ・テキサス・システム 疾患の処置のためのエキソソームの使用
MA44908A (fr) 2015-09-08 2018-07-18 Sylentis Sau Molécules d'arnsi et leur utilisation dans des procédés et des compositions pour inhiber l'expression du gène nrarp
US10383935B2 (en) 2015-09-23 2019-08-20 Regents Of The University Of Minnesota Methods of making and using live attenuated viruses
CA2999916C (en) 2015-09-23 2021-07-20 Massachusetts Institute Of Technology Compositions and methods for modified dendrimer nanoparticle vaccine delivery
US10086063B2 (en) 2015-09-23 2018-10-02 Regents Of The University Of Minnesota Methods of making and using live attenuated viruses
CA2997444A1 (en) 2015-09-29 2017-04-06 Amgen Inc. Asgr inhibitors for reducing cholesterol levels
JOP20210043A1 (ar) * 2015-10-01 2017-06-16 Arrowhead Pharmaceuticals Inc تراكيب وأساليب لتثبيط تعبير جيني للـ lpa
CN117503905A (zh) 2015-10-07 2024-02-06 阿佩利斯制药有限公司 给药方案
WO2017085550A1 (en) 2015-11-16 2017-05-26 Olix Pharmaceuticals, Inc. Treatment of age-related macular degeneration using rna complexes that target myd88 or tlr3
WO2017095751A1 (en) 2015-12-02 2017-06-08 Partikula Llc Compositions and methods for modulating cancer cell metabolism
ES2844899T3 (es) 2015-12-10 2021-07-23 Fibrogen Inc Métodos para el tratamiento de enfermedades de las neuronas motoras
PT3391875T (pt) 2015-12-18 2021-12-20 Samyang Holdings Corp Método para preparar micelas poliméricas contendo fármaco aniónico
BR102017001164A2 (pt) 2016-01-26 2019-03-06 Embrapa - Empresa Brasileira De Pesquisa Agropecuária Composições de rna de fita dupla para controle de diaphorina citri e métodos de uso.
JP2019503394A (ja) 2016-01-31 2019-02-07 ユニバーシティ・オブ・マサチューセッツＵｎｉｖｅｒｓｉｔｙ Ｏｆ Ｍａｓｓａｃｈｕｓｅｔｔｓ 分岐オリゴヌクレオチド
JP7003044B2 (ja) 2016-02-02 2022-01-20 オリックス ファーマシューティカルズ，インコーポレーテッド Ａｎｇｐｔ２およびｐｄｇｆｂを標的化するｒｎａ複合体を用いる血管新生関連疾患の処置
CA3022874A1 (en) 2016-02-02 2017-08-10 Olix Pharmaceuticals, Inc. Treatment of atopic dermatitis and asthma using rna complexes that target il4ra, trpa1, or f2rl1
SG11201806868TA (en) 2016-02-25 2018-09-27 Applied Biological Laboratories Inc Compositions and methods for protecting against airborne pathogens and irritants
US20170360815A1 (en) 2016-02-25 2017-12-21 Applied Biological Laboratories, Inc. Compositions and methods for protecting against airborne pathogens and irritants
WO2017147594A1 (en) 2016-02-26 2017-08-31 Yale University COMPOSITIONS AND METHODS OF USING piRNAS IN CANCER DIAGNOSTICS AND THERAPEUTICS
WO2017151623A1 (en) 2016-03-01 2017-09-08 Alexion Pharmaceuticals, Inc. Biodegradable activated polymers for therapeutic delivery
EP3423568A4 (en) 2016-03-04 2019-11-13 University Of Louisville Research Foundation, Inc. METHOD AND COMPOSITIONS FOR EX-VIVO REPRODUCTION OF VERY SMALL EMBRYONAL STEM CELLS (VSELS)
MX2018011204A (es) 2016-03-15 2019-03-07 Mersana Therapeutics Inc Conjugados de anticuerpo-farmaco dirigidos a napi2b y sus metodos de uso.
MA45328A (fr) 2016-04-01 2019-02-06 Avidity Biosciences Llc Compositions acide nucléique-polypeptide et utilisations de celles-ci
MA45469A (fr) 2016-04-01 2019-02-06 Avidity Biosciences Llc Acides nucléiques de bêta-caténine et leurs utilisations
MA45349A (fr) 2016-04-01 2019-02-06 Avidity Biosciences Llc Acides nucléiques egfr et leurs utilisations
MA45470A (fr) 2016-04-01 2019-02-06 Avidity Biosciences Llc Acides nucléiques kras et leurs utilisations
WO2017173453A1 (en) 2016-04-01 2017-10-05 The Brigham And Women's Hospital, Inc. Stimuli-responsive nanoparticles for biomedical applications
EP3440090B1 (en) 2016-04-06 2022-09-28 Ohio State Innovation Foundation Rna ligand-displaying exosomes for specific delivery of therapeutics to cell by rna nanotechnology
KR102339886B1 (ko) 2016-04-11 2021-12-17 올릭스 주식회사 연결 조직 성장 인자를 표적화하는 rna 복합체를 사용한 특발성 폐 섬유증의 치료 방법
JP7236195B2 (ja) * 2016-04-14 2023-03-09 ベニテック アイピー ホールディングス インコーポレーテッド 眼咽頭筋ジストロフィー（ｏｐｍｄ）の処置のための試薬およびその使用
WO2017189870A1 (en) 2016-04-27 2017-11-02 Massachusetts Institute Of Technology Stable nanoscale nucleic acid assemblies and methods thereof
WO2017197128A1 (en) 2016-05-11 2017-11-16 Yale University Poly(amine-co-ester) nanoparticles and methods of use thereof
KR101916652B1 (ko) 2016-06-29 2018-11-08 올릭스 주식회사 작은 간섭 rna의 rna 간섭효과 증진용 화합물 및 이의 용도
AU2017290828A1 (en) 2016-06-30 2019-01-24 Virogin Biotech Canada Ltd Pseudotyped oncolytic viral delivery of therapeutic polypeptides
RU2627179C1 (ru) * 2016-07-28 2017-08-03 федеральное государственное бюджетное учреждение "Федеральный научно-исследовательский центр эпидемиологии и микробиологии имени почетного академика Н.Ф. Гамалеи" Министерства здравоохранения Российской Федерации ТЕСТ-СИСТЕМА ДЛЯ ОПРЕДЕЛЕНИЯ РНК ИНТЕРФЕРОНА λ, ИНТЕРЛЕЙКИНА IL23 И ПРОТИВОВИРУСНОГО БЕЛКА MxA
EP3519582A1 (en) 2016-07-29 2019-08-07 Danmarks Tekniske Universitet Methods for decoupling cell growth from production of biochemicals and recombinant polypeptides
US11123435B2 (en) 2016-08-03 2021-09-21 H. Lee Moffitt Cancer Center And Research Institute, Inc. TLR9 targeted therapeutics
WO2018039629A2 (en) 2016-08-25 2018-03-01 Northwestern University Micellar spherical nucleic acids from thermoresponsive, traceless templates
CN110072530A (zh) 2016-09-02 2019-07-30 迪克纳制药公司 4′-磷酸酯类似物和包含其的寡核苷酸
EP3516062A1 (en) 2016-09-21 2019-07-31 Alnylam Pharmaceuticals, Inc. Myostatin irna compositions and methods of use thereof
EP3521430A4 (en) * 2016-09-29 2020-05-20 National University Corporation Tokyo Medical and Dental University DOUBLE-STRANDED NUCLEIC ACID COMPLEX WITH OVERHEAD
WO2018098352A2 (en) 2016-11-22 2018-05-31 Jun Oishi Targeting kras induced immune checkpoint expression
US11135307B2 (en) 2016-11-23 2021-10-05 Mersana Therapeutics, Inc. Peptide-containing linkers for antibody-drug conjugates
WO2018112470A1 (en) 2016-12-16 2018-06-21 The Brigham And Women's Hospital, Inc. Co-delivery of nucleic acids for simultaneous suppression and expression of target genes
WO2018132432A1 (en) 2017-01-10 2018-07-19 Arrowhead Pharmaceuticals, Inc. Alpha-1 antitrypsin (aat) rnai agents, compositions including aat rnai agents, and methods of use
US11591600B2 (en) 2017-02-10 2023-02-28 OliX Pharmaceuticals. Inc. Long double-stranded RNA for RNA interference
DE102017103383A1 (de) 2017-02-20 2018-08-23 aReNA-Bio GbR (vertretungsberechtigter Gesellschafter: Dr. Heribert Bohlen, 50733 Köln) System und Verfahren zur Zelltyp-spezifischen Translation von RNA-Molekülen in Eukaryoten
WO2018152523A1 (en) * 2017-02-20 2018-08-23 Northwestern University Use of trinucleotide repeat rnas to treat cancer
TW201834697A (zh) 2017-02-28 2018-10-01 美商梅爾莎納醫療公司 Her2標靶抗體－藥物結合物之組合療法
CA3059304A1 (en) 2017-04-07 2018-10-11 Apellis Pharmaceuticals, Inc. Dosing regimens and related compositions and methods
WO2018191751A1 (en) 2017-04-14 2018-10-18 Arizona Board Of Regents On Behalf Of The University Of Arizonia Compositions and methods for treating pulmonary fibrosis
US11433131B2 (en) 2017-05-11 2022-09-06 Northwestern University Adoptive cell therapy using spherical nucleic acids (SNAs)
EP3642341A4 (en) * 2017-06-23 2021-06-16 University Of Massachusetts TWO-DAY SELF-RELEASING SIRNA AND RELATED PROCEDURES
US11110114B2 (en) 2017-07-17 2021-09-07 Oxford University Innovation Limited Dinucleotides
US11104700B2 (en) 2017-07-17 2021-08-31 Oxford University Innovation Limited Oligonucleotides
WO2019068326A1 (en) 2017-10-05 2019-04-11 Université D'aix-Marseille INHIBITORS OF LSD1 FOR THE TREATMENT AND PREVENTION OF CARDIOMYOPATHIES
JP7353276B2 (ja) 2017-10-20 2023-09-29 ディセルナ ファーマシューティカルズ インコーポレイテッド Ｂ型肝炎感染の治療方法
EP3713644A1 (en) 2017-11-20 2020-09-30 University of Georgia Research Foundation, Inc. Compositions and methods for modulating hif-2& x391; to improve muscle generation and repair
WO2019104289A1 (en) 2017-11-27 2019-05-31 Mersana Therapeutics, Inc. Pyrrolobenzodiazepine antibody conjugates
WO2019113393A1 (en) 2017-12-06 2019-06-13 Avidity Biosciences Llc Compositions and methods of treating muscle atrophy and myotonic dystrophy
WO2019126651A1 (en) 2017-12-21 2019-06-27 Alnylam Pharmaceuticals, Inc. Chirally-enriched double-stranded rna agents
EP3727463A1 (en) 2017-12-21 2020-10-28 Mersana Therapeutics, Inc. Pyrrolobenzodiazepine antibody conjugates
US10960086B2 (en) 2017-12-28 2021-03-30 Augusta University Research Institute, Inc. Aptamer compositions and methods of use thereof
WO2019133847A1 (en) 2017-12-29 2019-07-04 Oncorus, Inc. Oncolytic viral delivery of therapeutic polypeptides
JP2021509669A (ja) 2018-01-05 2021-04-01 ディセルナ ファーマシューティカルズ インコーポレイテッド 免疫療法を強化するためのベータ−カテニン及びｉｄｏの発現の低減
US11661603B2 (en) 2018-01-16 2023-05-30 Dicerna Pharmaceuticals, Inc. Compositions and methods for inhibiting ALDH2 expression
SG11202007093YA (en) 2018-02-09 2020-08-28 Genentech Inc Oligonucleotides for modulating tmem106b expression
JP2021522816A (ja) 2018-05-07 2021-09-02 ロシュ イノベーション センター コペンハーゲン エーエス オリゴヌクレオチド治療のための超並列的探索方法
US20210246507A1 (en) 2018-05-10 2021-08-12 The University Of Manchester Methods for assessing macular degeneration
US20210205264A1 (en) 2018-09-04 2021-07-08 H. Lee Moffitt Cancer Center & Research Institute Inc. Use of delta-tocotrienol for treating cancer
WO2020051507A1 (en) 2018-09-06 2020-03-12 The Broad Institute, Inc. Nucleic acid assemblies for use in targeted delivery
US20230021500A1 (en) 2018-10-29 2023-01-26 Mersana Therapeutics, Inc. Cysteine engineered antibody-drug conjugates with peptide-containing linkers
MX2021009754A (es) 2019-02-12 2021-09-08 Dicerna Pharmaceuticals Inc Metodos y composiciones para inhibir la expresion de cyp27a1.
US20220154189A1 (en) 2019-03-29 2022-05-19 Dicerna Pharmaceuticals, Inc. Compositions and methods for the treatment of kras associated diseases or disorders
JP2022526419A (ja) 2019-04-04 2022-05-24 ディセルナ ファーマシューティカルズ インコーポレイテッド 中枢神経系における遺伝子発現を阻害するための組成物及び方法
US11814464B2 (en) 2019-04-29 2023-11-14 Yale University Poly(amine-co-ester) polymers and polyplexes with modified end groups and methods of use thereof
CN113795581A (zh) 2019-05-03 2021-12-14 迪克纳制药公司 具有缩短的有义链的双链核酸抑制剂分子
US20200369759A1 (en) 2019-05-23 2020-11-26 Fibrogen, Inc. Methods of treatment of muscular dystrophies
EP3990028A4 (en) 2019-06-26 2023-08-09 Biorchestra Co., Ltd. MICELLAR NANOPARTICLES AND ASSOCIATED USES
KR20220101073A (ko) 2019-08-30 2022-07-19 예일 유니버시티 핵산을 세포로 전달하기 위한 조성물 및 방법
CN114616332A (zh) 2019-09-10 2022-06-10 第一三共株式会社 用于递送至肝脏的GalNAc-寡核苷酸偶联物及其制备方法
WO2021067744A1 (en) 2019-10-02 2021-04-08 Dicerna Pharmaceuticals, Inc. Chemical modifications of small interfering rna with minimal fluorine content
US11017851B1 (en) 2019-11-26 2021-05-25 Cypress Semiconductor Corporation Silicon-oxide-nitride-oxide-silicon based multi level non-volatile memory device and methods of operation thereof
JP2023509872A (ja) 2019-12-24 2023-03-10 エフ． ホフマン－ラ ロシュ アーゲー Ｈｂｖを標的とする抗ウイルス剤及び／又はｈｂｖの処置のための免疫調節剤の医薬組合せ
MX2022007908A (es) 2019-12-24 2022-07-21 Hoffmann La Roche Combinacion farmaceutica de un oligonucleotido terapeutico que actua sobre hbv y un agonista de tlr7 para el tratamiento de hbv.
CN115315273A (zh) 2020-01-14 2022-11-08 辛德凯因股份有限公司 Il-2直向同源物及其使用方法
WO2021150300A1 (en) 2020-01-22 2021-07-29 Massachusetts Institute Of Technology Inducible tissue constructs and uses thereof
WO2021173965A1 (en) 2020-02-28 2021-09-02 Massachusetts Institute Of Technology Identification of variable influenza residues and uses thereof
KR20220156880A (ko) 2020-03-18 2022-11-28 다이서나 파마수이티컬, 인크. Angptl3 발현을 저해하기 위한 조성물 및 방법
AU2021237465A1 (en) 2020-03-19 2022-10-13 Avidity Biosciences, Inc. Compositions and methods of treating Facioscapulohumeral muscular dystrophy
JP2023527638A (ja) 2020-03-27 2023-06-30 アビディティー バイオサイエンシーズ，インク． 顔面肩甲上腕型筋ジストロフィーを処置するための組成物および方法
EP4139440A1 (en) 2020-04-22 2023-03-01 Iovance Biotherapeutics, Inc. Systems and methods for coordinating manufacturing of cells for patient-specific immunotherapy
CA3183351A1 (en) 2020-06-19 2021-12-23 Yale University Poly(amine-co-ester) polymers with modified end groups and enhanced pulmonary delivery
WO2021255262A1 (en) 2020-06-19 2021-12-23 Sylentis Sau siRNA AND COMPOSITIONS FOR PROPHYLACTIC AND THERAPEUTIC TREATMENT OF VIRUS DISEASES
US20220031633A1 (en) 2020-07-28 2022-02-03 Yale University Poly(amine-co-ester) polymeric particles for selective pulmonary delivery
IL300286A (en) 2020-08-04 2023-04-01 Dicerna Pharmaceuticals Inc Compounds and methods for inhibiting PLP1 expression
TW202221120A (zh) 2020-08-04 2022-06-01 美商黛瑟納製藥公司 用於治療代謝症候群之組成物及方法
AU2021321289A1 (en) 2020-08-04 2023-03-02 Dicerna Pharmaceuticals, Inc. Systemic delivery of oligonucleotides
IL300340A (en) 2020-08-05 2023-04-01 Hoffmann La Roche Treatment with oligonucleotides in hepatitis B patients
BR112023001957A2 (pt) 2020-08-05 2023-04-25 Dicerna Pharmaceuticals Inc Composições e métodos para inibir expressão de lpa
WO2022047424A1 (en) 2020-08-31 2022-03-03 Yale University Compositions and methods for delivery of nucleic acids to cells
EP3964204A1 (en) 2020-09-08 2022-03-09 Université d'Aix-Marseille Lsd1 inhibitors for use in the treatment and prevention of fibrosis of tissues
WO2022058447A1 (en) 2020-09-16 2022-03-24 The University Of Manchester Complementome assay
CN117295753A (zh) 2020-12-04 2023-12-26 基那奥生物公司 用于将核酸递送到细胞的组合物和方法
EP4015634A1 (en) 2020-12-15 2022-06-22 Sylentis, S.A.U. Sirna and compositions for prophylactic and therapeutic treatment of virus diseases
CN117202894A (zh) 2021-03-31 2023-12-08 胭脂红治疗私人有限公司 负载至少两个不同核酸的细胞外囊泡
KR20230170732A (ko) 2021-04-12 2023-12-19 베링거 인겔하임 인터내셔날 게엠베하 케토헥소키나제 (khk)를 억제하기 위한 조성물 및 방법
EP4323519A1 (en) 2021-04-14 2024-02-21 Dicerna Pharmaceuticals, Inc. Compositions and methods for modulating pnpla3 expression
WO2022223515A2 (en) 2021-04-19 2022-10-27 Novo Nordisk A/S Compositions and methods for inhibiting nuclear receptor subfamily 1 group h member 3 (nr1h3) expression
AU2022282579A1 (en) 2021-05-28 2023-11-02 Dicerna Pharmaceuticals, Inc. Compositions and methods for inhibiting mitochondria amidoxime reducing component 1 (marc1) expression
AU2022285661A1 (en) 2021-05-29 2023-12-21 1Globe Health Institute Llc Asymmetric short duplex dna as a novel gene silencing technology and use thereof
WO2022256354A1 (en) 2021-05-29 2022-12-08 1Globe Health Institute Llc Short duplex dna as a novel gene silencing technology and use thereof
BR112023026862A2 (pt) 2021-06-23 2024-03-05 Beth Israel Deaconess Medical Ct Inc Compostos de oligonucleotídeos anti-flt1 otimizados para tratamento de pré-eclâmpsia e outros distúrbios angiogênicos
CA3229305A1 (en) 2021-08-16 2023-02-23 Vib Vzw Oligonucleotides for modulating synaptogyrin-3 expression
US20230107967A1 (en) 2021-08-25 2023-04-06 Dicerna Pharmaceuticals, Inc. Compositions and methods for inhibiting alpha-1 antitrypsin expression
CA3232054A1 (en) 2021-09-21 2023-03-30 Kannan Rangaramanujam Dendrimer conjugates of small molecule biologics for intracellular delivery
WO2023083906A2 (en) 2021-11-11 2023-05-19 F. Hoffmann-La Roche Ag Pharmaceutical combinations for treatment of hbv
WO2023092089A1 (en) 2021-11-19 2023-05-25 Kist (Korea Institute Of Science And Technology) Therapeutic compounds for red blood cell-mediated delivery of an active pharmaceutical ingredient to a target cell
AR127843A1 (es) 2021-12-01 2024-03-06 Dicerna Pharmaceuticals Inc Composiciones y métodos para modular la expresión de apoc3
WO2023118546A2 (en) 2021-12-23 2023-06-29 Boehringer Ingelheim International Gmbh METHODS AND MOLECULES FOR RNA INTERFERENCE (RNAi)
WO2023159189A1 (en) 2022-02-18 2023-08-24 Yale University Branched poly(amine-co-ester) polymers for more efficient nucleic expression
GB202203627D0 (en) 2022-03-16 2022-04-27 Univ Manchester Agents for treating complement-related disorders
WO2023192872A1 (en) 2022-03-28 2023-10-05 Massachusetts Institute Of Technology Rna scaffolded wireframe origami and methods thereof
GB202204884D0 (en) 2022-04-04 2022-05-18 Fondo Ricerca Medica S R I Sirna targeting kcna1
US20230374522A1 (en) 2022-04-15 2023-11-23 Dicerna Pharmaceuticals, Inc. Compositions and methods for modulating scap activity
TW202400792A (zh) 2022-05-12 2024-01-01 美商戴瑟納製藥股份有限公司 用於抑制ｍａｐｔ表現之組合物及方法
WO2023220351A1 (en) 2022-05-13 2023-11-16 Dicerna Pharmaceuticals, Inc. Compositions and methods for inhibiting snca expression
TW202400193A (zh) 2022-06-24 2024-01-01 丹麥商諾佛 儂迪克股份有限公司 抑制跨膜絲胺酸蛋白酶６（ｔｍｐｒｓｓ６）表現的組成物及方法
WO2024040041A1 (en) 2022-08-15 2024-02-22 Dicerna Pharmaceuticals, Inc. Regulation of activity of rnai molecules
WO2024081736A2 (en) 2022-10-11 2024-04-18 Yale University Compositions and methods of using cell-penetrating antibodies

Citations (72)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4469863A (en) * 1980-11-12 1984-09-04 Ts O Paul O P Nonionic nucleic acid alkyl and aryl phosphonates and processes for manufacture and use thereof
US5208149A (en) * 1983-10-20 1993-05-04 The Research Foundation Of State University Of New York Nucleic acid constructs containing stable stem and loop structures
US5457189A (en) * 1989-12-04 1995-10-10 Isis Pharmaceuticals Antisense oligonucleotide inhibition of papillomavirus
US5514577A (en) * 1990-02-26 1996-05-07 Isis Pharmaceuticals, Inc. Oligonucleotide therapies for modulating the effects of herpes viruses
US5576208A (en) * 1991-06-14 1996-11-19 Isis Pharmaceuticals Inc. Antisense oligonucleotide inhibition of the RAS gene
US5578716A (en) * 1993-12-01 1996-11-26 Mcgill University DNA methyltransferase antisense oligonucleotides
US5580859A (en) * 1989-03-21 1996-12-03 Vical Incorporated Delivery of exogenous DNA sequences in a mammal
US5594122A (en) * 1993-06-23 1997-01-14 Genesys Pharma Inc. Antisense oligonucleotides targeted against human immunodeficiency virus
US5624808A (en) * 1995-03-28 1997-04-29 Becton Dickinson And Company Method for identifying cells committed to apoptosis by determining cellular phosphotyrosine content
US5624803A (en) * 1993-10-14 1997-04-29 The Regents Of The University Of California In vivo oligonucleotide generator, and methods of testing the binding affinity of triplex forming oligonucleotides derived therefrom
US5670633A (en) * 1990-01-11 1997-09-23 Isis Pharmaceuticals, Inc. Sugar modified oligonucleotides that detect and modulate gene expression
US5672695A (en) * 1990-10-12 1997-09-30 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Modified ribozymes
US5712257A (en) * 1987-08-12 1998-01-27 Hem Research, Inc. Topically active compositions of mismatched dsRNAs
US5770580A (en) * 1992-04-13 1998-06-23 Baylor College Of Medicine Somatic gene therapy to cells associated with fluid spaces
US5795715A (en) * 1991-12-18 1998-08-18 Cis Bio International Process for preparing double-stranded RNA, and its applications
US5801154A (en) * 1993-10-18 1998-09-01 Isis Pharmaceuticals, Inc. Antisense oligonucleotide modulation of multidrug resistance-associated protein
US5814500A (en) * 1996-10-31 1998-09-29 The Johns Hopkins University School Of Medicine Delivery construct for antisense nucleic acids and methods of use
US5898031A (en) * 1996-06-06 1999-04-27 Isis Pharmaceuticals, Inc. Oligoribonucleotides for cleaving RNA
US5908779A (en) * 1993-12-01 1999-06-01 University Of Connecticut Targeted RNA degradation using nuclear antisense RNA
US5919722A (en) * 1987-02-25 1999-07-06 Exxon Chemical Patents Inc. Zeolite L catalyst
US5972704A (en) * 1992-05-14 1999-10-26 Ribozyme Pharmaceuticals, Inc. HIV nef targeted ribozymes
US5998203A (en) * 1996-04-16 1999-12-07 Ribozyme Pharmaceuticals, Inc. Enzymatic nucleic acids containing 5'-and/or 3'-cap structures
US6001990A (en) * 1994-05-10 1999-12-14 The General Hospital Corporation Antisense inhibition of hepatitis C virus
US6057153A (en) * 1995-01-13 2000-05-02 Yale University Stabilized external guide sequences
US6225290B1 (en) * 1996-09-19 2001-05-01 The Regents Of The University Of California Systemic gene therapy by intestinal cell transformation
US20020086356A1 (en) * 2000-03-30 2002-07-04 Whitehead Institute For Biomedical Research RNA sequence-specific mediators of RNA interference
US20020114784A1 (en) * 1999-01-28 2002-08-22 Medical College Of Georgia Research Institute, Inc. Composition and method for in vivo and in vitro attenuation of gene expression using double stranded RNA
US20020132257A1 (en) * 2001-01-31 2002-09-19 Tony Giordano Use of post-transcriptional gene silencing for identifying nucleic acid sequences that modulate the function of a cell
US20020137210A1 (en) * 1999-12-09 2002-09-26 Churikov Nikolai Andreevich Method for modifying genetic characteristics of an organism
US20020162126A1 (en) * 2000-03-16 2002-10-31 David Beach Methods and compositions for RNA interference
US20020160393A1 (en) * 2000-12-28 2002-10-31 Symonds Geoffrey P. Double-stranded RNA-mediated gene suppression
US6476205B1 (en) * 1989-10-24 2002-11-05 Isis Pharmaceuticals, Inc. 2′ Modified oligonucleotides
US6475726B1 (en) * 1998-01-09 2002-11-05 Cubist Pharmaceuticals, Inc. Method for identifying validated target and assay combinations for drug development
US6506559B1 (en) * 1997-12-23 2003-01-14 Carnegie Institute Of Washington Genetic inhibition by double-stranded RNA
US6531647B1 (en) * 1997-09-22 2003-03-11 Plant Bioscience Limited Gene silencing methods
US20030064945A1 (en) * 1997-01-31 2003-04-03 Saghir Akhtar Enzymatic nucleic acid treatment of diseases or conditions related to levels of epidermal growth factor receptors
US20030068301A1 (en) * 1992-05-14 2003-04-10 Kenneth Draper Method and reagent for inhibiting hepatitis B virus replication
US20030084471A1 (en) * 2000-03-16 2003-05-01 David Beach Methods and compositions for RNA interference
US20030140362A1 (en) * 2001-06-08 2003-07-24 Dennis Macejak In vivo models for screening inhibitors of hepatitis B virus
US20030148985A1 (en) * 2001-12-05 2003-08-07 David Morrissey Methods and reagents for the inhibition of hepatitis B virus replication
US20030153521A1 (en) * 2001-05-29 2003-08-14 Mcswiggen James Nucleic acid treatment of diseases or conditions related to levels of Ras
US20030171311A1 (en) * 1998-04-27 2003-09-11 Lawrence Blatt Enzymatic nucleic acid treatment of diseases or conditions related to hepatitis C virus infection
US20030180756A1 (en) * 2002-03-21 2003-09-25 Yang Shi Compositions and methods for suppressing eukaryotic gene expression
US20030190654A1 (en) * 2002-01-22 2003-10-09 Ribopharma Double-stranded RNA (dsRNA) and method of use for inhibiting expression of a fusion gene
US6635805B1 (en) * 1997-02-14 2003-10-21 Plant Bioscience Limited Methods and DNA constructs for gene silencing in transgenic plants
US20030206887A1 (en) * 1992-05-14 2003-11-06 David Morrissey RNA interference mediated inhibition of hepatitis B virus (HBV) using short interfering nucleic acid (siNA)
US20040002153A1 (en) * 1999-07-21 2004-01-01 Monia Brett P. Modulation of PTEN expression via oligomeric compounds
US20040001811A1 (en) * 2001-01-09 2004-01-01 Ribopharma Ag Compositions and methods for inhibiting expression of anti-apoptotic genes
US20040006035A1 (en) * 2001-05-29 2004-01-08 Dennis Macejak Nucleic acid mediated disruption of HIV fusogenic peptide interactions
US20040005593A1 (en) * 2002-03-06 2004-01-08 Rigel Pharmaceuticals, Inc. Novel method for delivery and intracellular synthesis of siRNA molecules
US20040019001A1 (en) * 2002-02-20 2004-01-29 Mcswiggen James A. RNA interference mediated inhibition of protein typrosine phosphatase-1B (PTP-1B) gene expression using short interfering RNA
US20040038921A1 (en) * 2001-10-26 2004-02-26 Ribopharma Ag Composition and method for inhibiting expression of a target gene
US20040053876A1 (en) * 2002-03-26 2004-03-18 The Regents Of The University Of Michigan siRNAs and uses therof
US20040054156A1 (en) * 1992-05-14 2004-03-18 Kenneth Draper Method and reagent for inhibiting hepatitis B viral replication
US20040053875A1 (en) * 1999-01-30 2004-03-18 Ribopharma Ag Method and medicament for inhibiting the expression of a given gene
US20040096843A1 (en) * 2002-02-14 2004-05-20 Rossi John J. Methods for producing interfering RNA molecules in mammalian cells and therapeutic uses for such molecules
US6753139B1 (en) * 1999-10-27 2004-06-22 Plant Bioscience Limited Gene silencing
US20040121348A1 (en) * 2001-10-26 2004-06-24 Ribopharma Ag Compositions and methods for treating pancreatic cancer
US20040137471A1 (en) * 2002-09-18 2004-07-15 Timothy Vickers Efficient reduction of target RNA's by single-and double-stranded oligomeric compounds
US20040175703A1 (en) * 1999-11-24 2004-09-09 Ribopharma Ag Compositions and methods for inhibiting expression of a target gene
US20040191905A1 (en) * 2002-11-22 2004-09-30 University Of Massachusetts Modulation of HIV replication by RNA interference
US20040192626A1 (en) * 2002-02-20 2004-09-30 Mcswiggen James RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US20040203145A1 (en) * 2002-08-07 2004-10-14 University Of Massachusetts Compositions for RNA interference and methods of use thereof
US20040214330A1 (en) * 1999-04-07 2004-10-28 Waterhouse Peter Michael Methods and means for obtaining modified phenotypes
US20040224328A1 (en) * 2003-01-15 2004-11-11 Hans Prydz siRNA screening method
US20040229266A1 (en) * 2000-12-01 2004-11-18 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. RNA interference mediating small RNA molecules
US20040231016A1 (en) * 2003-02-19 2004-11-18 Commonwealth Scientific And Industrial Research Organization Efficient gene silencing in plants using short dsRNA sequences
US20040241854A1 (en) * 2002-08-05 2004-12-02 Davidson Beverly L. siRNA-mediated gene silencing
US20040248296A1 (en) * 2002-03-20 2004-12-09 Beresford Paul J. HIV therapeutic
US20040248835A1 (en) * 2001-10-26 2004-12-09 Anja Krebs Use of a double-stranded ribonucleic acid for treating an infection with a positivestrand rna-virus
US20050282765A1 (en) * 1996-10-04 2005-12-22 The Corporation Of The Trustees Of The Order Of The Sisters Of Mercy In Queensland Enzyme having S-adenosyl-L-homocysteine hydrolase (AHCY) type activity
US7232806B2 (en) * 2001-09-28 2007-06-19 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V. MicroRNA molecules

Family Cites Families (64)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2003006A (en) * 1933-04-11 1935-05-28 Michelson Barnett Samuel Water tank cover
IE66830B1 (en) 1987-08-12 1996-02-07 Hem Res Inc Topically active compositions of double-stranded RNAs
DE69133405T2 (de) 1990-01-11 2005-07-07 Isis Pharmaceutical, Inc., Carlsbad Oligonukleotidderivate zur detektieren und modulation von rna aktivität und genexpression
FR2675803B1 (fr) 1991-04-25 1996-09-06 Genset Sa Oligonucleotides fermes, antisens et sens et leurs applications.
CA2131311C (en) 1992-03-05 2004-06-29 Phillip Dan Cook Covalently cross-linked oligonucleotides
ATE171210T1 (de) 1992-07-02 1998-10-15 Hybridon Inc Selbststabilisierte oligonukleotide als therapeutika
US5652355A (en) 1992-07-23 1997-07-29 Worcester Foundation For Experimental Biology Hybrid oligonucleotide phosphorothioates
AU6080294A (en) 1992-12-31 1994-08-15 Texas Biotechnology Corporation Antisense molecules directed against genes of the (raf) oncogene family
US6056704A (en) 1993-03-03 2000-05-02 Ide; Masatake Foot-pressure massage stand
EP0616026A1 (en) 1993-03-19 1994-09-21 The Procter & Gamble Company Concentrated cleaning compositions
FR2710074B1 (fr) 1993-09-15 1995-12-08 Rhone Poulenc Rorer Sa Gène GRB3-3, ses variants et leurs utilisations.
KR960705589A (ko) 1993-11-16 1996-11-08 라이오넬 엔, 사이몬 키메라형 올리고뉴클레오시드 화합물(Chimeric Oligonucleoside Compounds)
US5674683A (en) 1995-03-21 1997-10-07 Research Corporation Technologies, Inc. Stem-loop and circular oligonucleotides and method of using
IL122290A0 (en) 1995-06-07 1998-04-05 Inex Pharmaceuticals Corp Lipid-nucleic acid complex its preparation and use
AU7366296A (en) * 1995-09-20 1997-04-09 Worcester Foundation For Biomedical Research, Inc. Antisense oligonucleotide chemotherapy for benign hyperplasia or cancer of the prostate
SK109198A3 (en) 1996-02-14 1999-06-11 Isis Pharmaceuticals Inc Sugar-modified gapped oligonucleotides
EP0910634A2 (en) 1996-04-17 1999-04-28 Hoechst Marion Roussel Deutschland GmbH ANTISENSE INHIBITORS OF VASCULAR ENDOTHELIAL GROWTH FACTOR (VEgF/VPF) EXPRESSION
DE19618797C2 (de) 1996-05-10 2000-03-23 Bertling Wolf Vehikel zum Transport molekularer Substanz
US20040266706A1 (en) 2002-11-05 2004-12-30 Muthiah Manoharan Cross-linked oligomeric compounds and their use in gene modulation
DE19631919C2 (de) 1996-08-07 1998-07-16 Deutsches Krebsforsch Anti-Sinn-RNA mit Sekundärstruktur
WO1998026062A2 (en) 1996-12-12 1998-06-18 Yissum Research Development Company Of The Hebrew University Of Jerusalem Synthetic antisense oligodeoxynucleotides and pharmaceutical compositions containing them
US6218142B1 (en) * 1997-03-05 2001-04-17 Michael Wassenegger Nucleic acid molecules encoding polypeptides having the enzymatic activity of an RNA-directed RNA polymerase (RDRP)
GB9710475D0 (en) 1997-05-21 1997-07-16 Zeneca Ltd Gene silencing
CN1273476C (zh) 1997-09-12 2006-09-06 埃克西康有限公司 寡核苷酸类似物
EP2292771A3 (en) 1997-09-19 2011-07-27 Life Technologies Corporation Sense mRNA therapy
CA2487328A1 (en) 1998-03-20 1999-09-30 Benitec Australia Ltd. Sirna for control of gene expression
AUPP249298A0 (en) * 1998-03-20 1998-04-23 Ag-Gene Australia Limited Synthetic genes and genetic constructs comprising same I
CN1202246C (zh) 1998-04-08 2005-05-18 联邦科学和工业研究组织 获得修饰表型的方法和措施
JP2003525017A (ja) 1998-04-20 2003-08-26 リボザイム・ファーマシューティカルズ・インコーポレーテッド 遺伝子発現を調節しうる新規な化学組成を有する核酸分子
AR020078A1 (es) 1998-05-26 2002-04-10 Syngenta Participations Ag Metodo para alterar la expresion de un gen objetivo en una celula de planta
GB9827152D0 (en) 1998-07-03 1999-02-03 Devgen Nv Characterisation of gene function using double stranded rna inhibition
AU1408300A (en) 1998-11-24 2000-06-13 Hisamitsu Pharmaceutical Co. Inc. Hiv infection inhibitors
WO2000032619A1 (en) 1998-11-30 2000-06-08 Ribogene, Inc. Methods and compositions for identification of inhibitors of ribosome assembly
US6939712B1 (en) 1998-12-29 2005-09-06 Impedagen, Llc Muting gene activity using a transgenic nucleic acid
BR0009884A (pt) 1999-04-21 2002-01-08 American Home Prod Processos e composições para a inibição da função das sequências de polinucleotìdeos
GB9927444D0 (en) 1999-11-19 2000-01-19 Cancer Res Campaign Tech Inhibiting gene expression
DE10160151A1 (de) 2001-01-09 2003-06-26 Ribopharma Ag Verfahren zur Hemmung der Expression eines vorgegebenen Zielgens
AU2001260114A1 (en) 2000-03-14 2001-09-24 Syngenta Participations Ag Protoporphyrinogen oxidase ("protox") genes
DK2028278T3 (da) 2000-03-30 2014-06-23 Univ Massachusetts RNA-sekvensspecifikke formidlere af RNA-interferens
CA2410947A1 (en) 2000-05-30 2001-12-06 Johnson & Johnson Research Pty Limited Methods for mediating gene suppresion by using factors that enhance rnai
US7033801B2 (en) 2000-12-08 2006-04-25 Invitrogen Corporation Compositions and methods for rapidly generating recombinant nucleic acid molecules
WO2003103600A2 (en) 2002-06-05 2003-12-18 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US7423142B2 (en) * 2001-01-09 2008-09-09 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of anti-apoptotic genes
WO2003035869A1 (de) 2001-10-26 2003-05-01 Ribopharma Ag Verwendung einer doppelsträngigen ribonukleinsäure zur gezielten hemmung der expression eines vorgegebenen zielgens
DE50101770D1 (de) 2001-06-01 2004-04-29 Mobilkom Austria Ag & Co Kg Wi Verfahren zur Bestimmung des Standortes einer Mobilstation in einem Mobilfunksystem
DE10163098B4 (de) 2001-10-12 2005-06-02 Alnylam Europe Ag Verfahren zur Hemmung der Replikation von Viren
DE10154113A1 (de) 2001-11-03 2003-05-15 Opel Adam Ag Frontstruktur eines Kraftfahrzeuges
US20030166282A1 (en) 2002-02-01 2003-09-04 David Brown High potency siRNAS for reducing the expression of target genes
WO2003099298A1 (en) 2002-05-24 2003-12-04 Max-Planck Gesellschaft zur Förderung der Wissenschaften e.V. Rna interference mediating small rna molecules
AU2003276666A1 (en) 2002-06-12 2003-12-31 Ambion, Inc. Methods and compositions relating to polypeptides with rnase iii domains that mediate rna interference
US8101348B2 (en) 2002-07-10 2012-01-24 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V. RNA-interference by single-stranded RNA molecules
EP2258847B2 (en) 2002-08-05 2020-07-01 Silence Therapeutics GmbH Futher novel forms of interfering RNA molecules
CA2500224C (en) 2002-09-25 2015-04-28 University Of Massachusetts In vivo gene silencing by chemically modified and stable sirna
AU2003290598A1 (en) 2002-11-05 2004-06-03 Isis Pharmaceuticals, Inc. Modified oligonucleotides for use in rna interference
EP2314691A3 (en) 2002-11-14 2012-01-18 Dharmacon, Inc. Fuctional and hyperfunctional siRNA
WO2004046324A2 (en) 2002-11-15 2004-06-03 University Of Massachusetts Allele-targeted rna interference
WO2004063375A1 (en) 2003-01-15 2004-07-29 Hans Prydz OPTIMIZING siRNA BY RNAi ANTISENSE
EP1583832B1 (en) 2003-01-17 2010-12-01 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Inducible small interfering rna (sirna) expression constructs for targeted gene silencing
WO2004065600A2 (en) 2003-01-17 2004-08-05 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Rna interference by palindromic or modified rna molecules
JP2006519008A (ja) 2003-02-10 2006-08-24 独立行政法人産業技術総合研究所 哺乳動物細胞の調節
EP3502252B1 (en) 2003-06-02 2023-04-05 University of Massachusetts Methods and compositions for controlling efficacy of rna silencing
US6998203B2 (en) * 2003-08-01 2006-02-14 Intel Corporation Proximity correcting lithography mask blanks
CA2594334A1 (en) 2005-01-07 2006-07-13 Alnylam Pharmaceuticals, Inc. Rnai modulation of rsv and therapeutic uses thereof
DE112012002886B4 (de) 2011-07-08 2022-12-29 Inteva Products Llc. Fahrzeuginnenraumkomponentennähverfahren und mit dem Verfahren erzeugtes Fahrzeuginnenraumverkleidungsstück

Patent Citations (95)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4469863A (en) * 1980-11-12 1984-09-04 Ts O Paul O P Nonionic nucleic acid alkyl and aryl phosphonates and processes for manufacture and use thereof
US5208149A (en) * 1983-10-20 1993-05-04 The Research Foundation Of State University Of New York Nucleic acid constructs containing stable stem and loop structures
US5919722A (en) * 1987-02-25 1999-07-06 Exxon Chemical Patents Inc. Zeolite L catalyst
US5712257A (en) * 1987-08-12 1998-01-27 Hem Research, Inc. Topically active compositions of mismatched dsRNAs
US5580859A (en) * 1989-03-21 1996-12-03 Vical Incorporated Delivery of exogenous DNA sequences in a mammal
US6476205B1 (en) * 1989-10-24 2002-11-05 Isis Pharmaceuticals, Inc. 2′ Modified oligonucleotides
US5457189A (en) * 1989-12-04 1995-10-10 Isis Pharmaceuticals Antisense oligonucleotide inhibition of papillomavirus
US5670633A (en) * 1990-01-11 1997-09-23 Isis Pharmaceuticals, Inc. Sugar modified oligonucleotides that detect and modulate gene expression
US5514577A (en) * 1990-02-26 1996-05-07 Isis Pharmaceuticals, Inc. Oligonucleotide therapies for modulating the effects of herpes viruses
US5672695A (en) * 1990-10-12 1997-09-30 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Modified ribozymes
US5576208A (en) * 1991-06-14 1996-11-19 Isis Pharmaceuticals Inc. Antisense oligonucleotide inhibition of the RAS gene
US5795715A (en) * 1991-12-18 1998-08-18 Cis Bio International Process for preparing double-stranded RNA, and its applications
US5770580A (en) * 1992-04-13 1998-06-23 Baylor College Of Medicine Somatic gene therapy to cells associated with fluid spaces
US5972704A (en) * 1992-05-14 1999-10-26 Ribozyme Pharmaceuticals, Inc. HIV nef targeted ribozymes
US20030068301A1 (en) * 1992-05-14 2003-04-10 Kenneth Draper Method and reagent for inhibiting hepatitis B virus replication
US20040054156A1 (en) * 1992-05-14 2004-03-18 Kenneth Draper Method and reagent for inhibiting hepatitis B viral replication
US20030206887A1 (en) * 1992-05-14 2003-11-06 David Morrissey RNA interference mediated inhibition of hepatitis B virus (HBV) using short interfering nucleic acid (siNA)
US5594122A (en) * 1993-06-23 1997-01-14 Genesys Pharma Inc. Antisense oligonucleotides targeted against human immunodeficiency virus
US5624803A (en) * 1993-10-14 1997-04-29 The Regents Of The University Of California In vivo oligonucleotide generator, and methods of testing the binding affinity of triplex forming oligonucleotides derived therefrom
US5801154A (en) * 1993-10-18 1998-09-01 Isis Pharmaceuticals, Inc. Antisense oligonucleotide modulation of multidrug resistance-associated protein
US5908779A (en) * 1993-12-01 1999-06-01 University Of Connecticut Targeted RNA degradation using nuclear antisense RNA
US5578716A (en) * 1993-12-01 1996-11-26 Mcgill University DNA methyltransferase antisense oligonucleotides
US6001990A (en) * 1994-05-10 1999-12-14 The General Hospital Corporation Antisense inhibition of hepatitis C virus
US6057153A (en) * 1995-01-13 2000-05-02 Yale University Stabilized external guide sequences
US5624808A (en) * 1995-03-28 1997-04-29 Becton Dickinson And Company Method for identifying cells committed to apoptosis by determining cellular phosphotyrosine content
US5998203A (en) * 1996-04-16 1999-12-07 Ribozyme Pharmaceuticals, Inc. Enzymatic nucleic acids containing 5'-and/or 3'-cap structures
US6107094A (en) * 1996-06-06 2000-08-22 Isis Pharmaceuticals, Inc. Oligoribonucleotides and ribonucleases for cleaving RNA
US5898031A (en) * 1996-06-06 1999-04-27 Isis Pharmaceuticals, Inc. Oligoribonucleotides for cleaving RNA
US6225290B1 (en) * 1996-09-19 2001-05-01 The Regents Of The University Of California Systemic gene therapy by intestinal cell transformation
US20050282765A1 (en) * 1996-10-04 2005-12-22 The Corporation Of The Trustees Of The Order Of The Sisters Of Mercy In Queensland Enzyme having S-adenosyl-L-homocysteine hydrolase (AHCY) type activity
US5814500A (en) * 1996-10-31 1998-09-29 The Johns Hopkins University School Of Medicine Delivery construct for antisense nucleic acids and methods of use
US20030064945A1 (en) * 1997-01-31 2003-04-03 Saghir Akhtar Enzymatic nucleic acid treatment of diseases or conditions related to levels of epidermal growth factor receptors
US6635805B1 (en) * 1997-02-14 2003-10-21 Plant Bioscience Limited Methods and DNA constructs for gene silencing in transgenic plants
US6531647B1 (en) * 1997-09-22 2003-03-11 Plant Bioscience Limited Gene silencing methods
US20030051263A1 (en) * 1997-12-23 2003-03-13 The Carnegie Institution Of Washington Genetic inhibition by double-stranded RNA
US20030055020A1 (en) * 1997-12-23 2003-03-20 The Carnegie Institution Of Washington Genetic inhibition by double-stranded RNA
US20030056235A1 (en) * 1997-12-23 2003-03-20 The Carnegie Institution Of Washington Genetic inhibition by double-stranded RNA
US6506559B1 (en) * 1997-12-23 2003-01-14 Carnegie Institute Of Washington Genetic inhibition by double-stranded RNA
US6475726B1 (en) * 1998-01-09 2002-11-05 Cubist Pharmaceuticals, Inc. Method for identifying validated target and assay combinations for drug development
US20030171311A1 (en) * 1998-04-27 2003-09-11 Lawrence Blatt Enzymatic nucleic acid treatment of diseases or conditions related to hepatitis C virus infection
US20020114784A1 (en) * 1999-01-28 2002-08-22 Medical College Of Georgia Research Institute, Inc. Composition and method for in vivo and in vitro attenuation of gene expression using double stranded RNA
US20040053875A1 (en) * 1999-01-30 2004-03-18 Ribopharma Ag Method and medicament for inhibiting the expression of a given gene
US20040102408A1 (en) * 1999-01-30 2004-05-27 Ribopharma Ag Method and medicament for inhibiting the expression of a given gene
US20040072779A1 (en) * 1999-01-30 2004-04-15 Ribopharma Ag Method and medicament for inhibiting the expression of a given gene
US20040214330A1 (en) * 1999-04-07 2004-10-28 Waterhouse Peter Michael Methods and means for obtaining modified phenotypes
US20040002153A1 (en) * 1999-07-21 2004-01-01 Monia Brett P. Modulation of PTEN expression via oligomeric compounds
US6753139B1 (en) * 1999-10-27 2004-06-22 Plant Bioscience Limited Gene silencing
US20040221337A1 (en) * 1999-10-27 2004-11-04 Baulcombe David C. Gene silencing
US20040175703A1 (en) * 1999-11-24 2004-09-09 Ribopharma Ag Compositions and methods for inhibiting expression of a target gene
US20020137210A1 (en) * 1999-12-09 2002-09-26 Churikov Nikolai Andreevich Method for modifying genetic characteristics of an organism
US20040086884A1 (en) * 2000-03-16 2004-05-06 Genetica, Inc. Methods and compositions for RNA interference
US20020162126A1 (en) * 2000-03-16 2002-10-31 David Beach Methods and compositions for RNA interference
US20040018999A1 (en) * 2000-03-16 2004-01-29 David Beach Methods and compositions for RNA interference
US20030084471A1 (en) * 2000-03-16 2003-05-01 David Beach Methods and compositions for RNA interference
US20070003963A1 (en) * 2000-03-30 2007-01-04 Whitehead Institute For Biomedical Research RNA sequence-specific mediators of RNA interference
US20070003961A1 (en) * 2000-03-30 2007-01-04 Whitehead Institute For Biomedical Research RNA sequence-specific mediators of RNA interference
US20030108923A1 (en) * 2000-03-30 2003-06-12 Whitehead Institute For Biomedical Research RNA sequence-specific mediators of RNA interference
US20080132461A1 (en) * 2000-03-30 2008-06-05 Whitehead Institute For Biomedical Research RNA sequence-specific mediators of RNA interference
US20070003962A1 (en) * 2000-03-30 2007-01-04 Whitehead Institute For Biomedical Research RNA sequence-specific mediators of RNA interference
US20020086356A1 (en) * 2000-03-30 2002-07-04 Whitehead Institute For Biomedical Research RNA sequence-specific mediators of RNA interference
US20070003960A1 (en) * 2000-03-30 2007-01-04 Whitehead Institute For Biomedical Research RNA sequence-specific mediators of RNA interference
US20050234007A1 (en) * 2000-12-01 2005-10-20 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. RNA interference mediating small RNA molecules
US20070093445A1 (en) * 2000-12-01 2007-04-26 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E. V. RNA interference mediating small RNA molecules
US7056704B2 (en) * 2000-12-01 2006-06-06 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V. RNA interference mediating small RNA molecules
US20050234006A1 (en) * 2000-12-01 2005-10-20 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. RNA interference mediating small RNA molecules
US20050026278A1 (en) * 2000-12-01 2005-02-03 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. RNA interference mediating small RNA molecules
US20040259248A1 (en) * 2000-12-01 2004-12-23 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. RNA interference mediating small RNA molecules
US7078196B2 (en) * 2000-12-01 2006-07-18 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften, E.V. RNA interference mediating small RNA molecules
US20040229266A1 (en) * 2000-12-01 2004-11-18 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. RNA interference mediating small RNA molecules
US20020160393A1 (en) * 2000-12-28 2002-10-31 Symonds Geoffrey P. Double-stranded RNA-mediated gene suppression
US20040001811A1 (en) * 2001-01-09 2004-01-01 Ribopharma Ag Compositions and methods for inhibiting expression of anti-apoptotic genes
US20020132257A1 (en) * 2001-01-31 2002-09-19 Tony Giordano Use of post-transcriptional gene silencing for identifying nucleic acid sequences that modulate the function of a cell
US20030153521A1 (en) * 2001-05-29 2003-08-14 Mcswiggen James Nucleic acid treatment of diseases or conditions related to levels of Ras
US20040006035A1 (en) * 2001-05-29 2004-01-08 Dennis Macejak Nucleic acid mediated disruption of HIV fusogenic peptide interactions
US20030140362A1 (en) * 2001-06-08 2003-07-24 Dennis Macejak In vivo models for screening inhibitors of hepatitis B virus
US7232806B2 (en) * 2001-09-28 2007-06-19 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V. MicroRNA molecules
US20040038921A1 (en) * 2001-10-26 2004-02-26 Ribopharma Ag Composition and method for inhibiting expression of a target gene
US20040248835A1 (en) * 2001-10-26 2004-12-09 Anja Krebs Use of a double-stranded ribonucleic acid for treating an infection with a positivestrand rna-virus
US20040126791A1 (en) * 2001-10-26 2004-07-01 Ribopharma Ag Compositions and methods for treating trail-resistant cancer cells
US20040121348A1 (en) * 2001-10-26 2004-06-24 Ribopharma Ag Compositions and methods for treating pancreatic cancer
US20030148985A1 (en) * 2001-12-05 2003-08-07 David Morrissey Methods and reagents for the inhibition of hepatitis B virus replication
US20030190654A1 (en) * 2002-01-22 2003-10-09 Ribopharma Double-stranded RNA (dsRNA) and method of use for inhibiting expression of a fusion gene
US20040096843A1 (en) * 2002-02-14 2004-05-20 Rossi John J. Methods for producing interfering RNA molecules in mammalian cells and therapeutic uses for such molecules
US20040192626A1 (en) * 2002-02-20 2004-09-30 Mcswiggen James RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US20040019001A1 (en) * 2002-02-20 2004-01-29 Mcswiggen James A. RNA interference mediated inhibition of protein typrosine phosphatase-1B (PTP-1B) gene expression using short interfering RNA
US20040005593A1 (en) * 2002-03-06 2004-01-08 Rigel Pharmaceuticals, Inc. Novel method for delivery and intracellular synthesis of siRNA molecules
US20040248296A1 (en) * 2002-03-20 2004-12-09 Beresford Paul J. HIV therapeutic
US20030180756A1 (en) * 2002-03-21 2003-09-25 Yang Shi Compositions and methods for suppressing eukaryotic gene expression
US20040053876A1 (en) * 2002-03-26 2004-03-18 The Regents Of The University Of Michigan siRNAs and uses therof
US20040241854A1 (en) * 2002-08-05 2004-12-02 Davidson Beverly L. siRNA-mediated gene silencing
US20040203145A1 (en) * 2002-08-07 2004-10-14 University Of Massachusetts Compositions for RNA interference and methods of use thereof
US20040137471A1 (en) * 2002-09-18 2004-07-15 Timothy Vickers Efficient reduction of target RNA's by single-and double-stranded oligomeric compounds
US20040191905A1 (en) * 2002-11-22 2004-09-30 University Of Massachusetts Modulation of HIV replication by RNA interference
US20040224328A1 (en) * 2003-01-15 2004-11-11 Hans Prydz siRNA screening method
US20040231016A1 (en) * 2003-02-19 2004-11-18 Commonwealth Scientific And Industrial Research Organization Efficient gene silencing in plants using short dsRNA sequences

Cited By (1247)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9096636B2 (en) 1996-06-06 2015-08-04 Isis Pharmaceuticals, Inc. Chimeric oligomeric compounds and their use in gene modulation
US7812149B2 (en) 1996-06-06 2010-10-12 Isis Pharmaceuticals, Inc. 2′-Fluoro substituted oligomeric compounds and compositions for use in gene modulations
US7695902B2 (en) 1996-06-06 2010-04-13 Isis Pharmaceuticals, Inc. Oligoribonucleotides and ribonucleases for cleaving RNA
US9758551B2 (en) 1999-10-20 2017-09-12 The Johns Hopkins University Superior molecular vaccine linking the translocation domain of a bacterial toxin to an antigen
US20040086845A1 (en) * 1999-10-20 2004-05-06 Tzyy-Choou Wu Superior molecular vaccine linking the translocation domain of a bacterial toxin to an antigen
US8128922B2 (en) 1999-10-20 2012-03-06 Johns Hopkins University Superior molecular vaccine linking the translocation domain of a bacterial toxin to an antigen
US8097710B2 (en) 1999-10-27 2012-01-17 Plant Bioscience Limited Gene silencing
US20050102709A1 (en) * 1999-10-27 2005-05-12 Plant Bioscience Limited RNA molecules and vectors for gene silencing
US20090286254A1 (en) * 1999-10-27 2009-11-19 David Charles Baulcombe Gene silencing
US20040221337A1 (en) * 1999-10-27 2004-11-04 Baulcombe David C. Gene silencing
US8759102B2 (en) 1999-10-27 2014-06-24 Plant Bioscience Limited Short RNA producing gene silencing in cells
US8263569B2 (en) 1999-10-27 2012-09-11 Plant Biosciences Limited Gene silencing
US8779236B2 (en) 1999-10-27 2014-07-15 Plant Bioscience Limited Gene silencing
US8258285B2 (en) 1999-10-27 2012-09-04 Plant Bioscience Limited RNA molecules and vectors for gene silencing
US20090288182A1 (en) * 1999-10-27 2009-11-19 David Charles Baulcombe Gene silencing
US20080312176A1 (en) * 1999-10-27 2008-12-18 David Charles Baulcombe Gene silencing
US20050102710A1 (en) * 1999-10-27 2005-05-12 Plant Bioscience Limited Cells and animals produced by gene silencing
US7704688B2 (en) 1999-10-27 2010-04-27 Plant Bioscience Limited Methods of detecting silencing mammalian cells
US8349607B2 (en) 1999-10-27 2013-01-08 Plant Bioscience Limited Gene silencing
US8299235B2 (en) 1999-10-27 2012-10-30 Plant Bioscience Limited RNA molecules and vectors for gene silencing
US20060168669A1 (en) * 1999-10-27 2006-07-27 Baulcombe David C Gene silencing
US9012621B2 (en) 2000-03-30 2015-04-21 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. RNA sequence-specific mediators of RNA interference
US20070003960A1 (en) * 2000-03-30 2007-01-04 Whitehead Institute For Biomedical Research RNA sequence-specific mediators of RNA interference
US20030108923A1 (en) * 2000-03-30 2003-06-12 Whitehead Institute For Biomedical Research RNA sequence-specific mediators of RNA interference
US8790922B2 (en) 2000-03-30 2014-07-29 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. RNA sequence-specific mediators of RNA interference
US8742092B2 (en) 2000-03-30 2014-06-03 University Of Massachusetts RNA sequence-specific mediators of RNA interference
US9012138B2 (en) 2000-03-30 2015-04-21 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. RNA sequence-specific mediators of RNA interference
US20090186843A1 (en) * 2000-03-30 2009-07-23 Whitehead Institute For Biomedical Research RNA sequence-specific mediators of RNA interference
US20080132461A1 (en) * 2000-03-30 2008-06-05 Whitehead Institute For Biomedical Research RNA sequence-specific mediators of RNA interference
US20070003963A1 (en) * 2000-03-30 2007-01-04 Whitehead Institute For Biomedical Research RNA sequence-specific mediators of RNA interference
US10472625B2 (en) 2000-03-30 2019-11-12 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. RNA sequence-specific mediators of RNA interference
US8632997B2 (en) 2000-03-30 2014-01-21 University Of Massachusetts RNA sequence-specific mediators of RNA interference
US9193753B2 (en) 2000-03-30 2015-11-24 University Of Massachusetts RNA sequence-specific mediators of RNA interference
US20020086356A1 (en) * 2000-03-30 2002-07-04 Whitehead Institute For Biomedical Research RNA sequence-specific mediators of RNA interference
US20070003962A1 (en) * 2000-03-30 2007-01-04 Whitehead Institute For Biomedical Research RNA sequence-specific mediators of RNA interference
US20070003961A1 (en) * 2000-03-30 2007-01-04 Whitehead Institute For Biomedical Research RNA sequence-specific mediators of RNA interference
US8394628B2 (en) 2000-03-30 2013-03-12 University Of Massachusetts RNA sequence-specific mediators of RNA interference
US8420391B2 (en) 2000-03-30 2013-04-16 University Of Massachusetts RNA sequence-specific mediators of RNA interference
US8552171B2 (en) * 2000-03-30 2013-10-08 University Of Massachusetts RNA sequence-specific mediators of RNA interference
US20120029061A1 (en) * 2000-03-30 2012-02-02 Thomas Tuschl Rna sequence-specific mediators of rna interference
US8372969B2 (en) 2000-08-02 2013-02-12 University Of Southern California RNA interference methods using DNA-RNA duplex constructs
US20110098461A1 (en) * 2000-08-02 2011-04-28 University Of Southern California Novel RNA Interference Methods Using DNA-RNA Duplex Constructs
US8080652B2 (en) * 2000-08-02 2011-12-20 University Of Southern California Gene silencing using mRNA-cDNA hybrids
US20100249212A1 (en) * 2000-08-02 2010-09-30 University Of Southern California Gene Silencing Using mRNA-cDNA Hybrids
US20080242627A1 (en) * 2000-08-02 2008-10-02 University Of Southern California Novel rna interference methods using dna-rna duplex constructs
US20090148471A1 (en) * 2000-08-03 2009-06-11 The Johns Hopkins University Molecular Vaccine Linking an Endoplasmic Reticulum Chaperone Polypeptide to an Antigen
US8007781B2 (en) 2000-08-03 2011-08-30 The Johns Hopkins University Molecular vaccine linking an endoplasmic reticulum chaperone polypeptide to an antigen
US20090149407A1 (en) * 2000-08-30 2009-06-11 Sirna Therapeutics, Inc. RNA INTERFERENCE MEDIATED TREATMENT OF ALZHEIMER'S DISEASE USING SHORT INTERFERING NUCLEIC ACID (siNA)
US20100168208A1 (en) * 2000-08-30 2010-07-01 Sima Therapeutics, Inc. RNA INTERFERENCE MEDIATED TREATMENT OF ALZHEIMER'S DISEASE USING SHORT INTERFERING NUCLEIC ACID (siNA)
US8017765B2 (en) 2000-08-30 2011-09-13 Merck Sharp & Dohme Corp. RNA interference mediated treatment of alzheimer's disease using short interfering nucleic acid (siNA)
US7662951B2 (en) * 2000-08-30 2010-02-16 Sirna Therapeutics, Inc. RNA interference mediated treatment of Alzheimer's disease using short interfering nucleic acid (siNA)
US20100144841A1 (en) * 2000-09-19 2010-06-10 University Of South Florida Control of nk cell function and survival by modulation of ship activity
US7713945B2 (en) * 2000-09-19 2010-05-11 University Of South Florida Control of NK cell function and survival by modulation of SHIP activity
US8163710B2 (en) 2000-09-19 2012-04-24 University Of South Florida Reduction of graft-versus-host disease by modulation of SHIP activity
US20080076731A1 (en) * 2000-09-19 2008-03-27 University Of South Florida Control of NK cell function and survival by modulation of ship activity
US8372968B2 (en) 2000-12-01 2013-02-12 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. RNA interference mediating small RNA molecules
US10633656B2 (en) 2000-12-01 2020-04-28 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. RNA interference mediating small RNA molecules
US8329463B2 (en) 2000-12-01 2012-12-11 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. RNA interference mediating small RNA molecules
US8993745B2 (en) 2000-12-01 2015-03-31 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. RNA interference mediating small RNA molecules
US20110070162A1 (en) * 2000-12-01 2011-03-24 Max-Phanck-Gesellschaft Zur Foerderung Der Wissenschaften E.V. Rna interference mediating small rna molecules
US8933044B2 (en) 2000-12-01 2015-01-13 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. RNA interference mediating small RNA molecules
US8765930B2 (en) 2000-12-01 2014-07-01 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. RNA interference mediating small RNA molecules
US20110054159A1 (en) * 2000-12-01 2011-03-03 Maxplanck-Gesellschaft Zur Foerderung Der Wissenschaften E.V. Rna interference mediating small rna molecules
US8778902B2 (en) 2000-12-01 2014-07-15 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. RNA interference mediating small RNA molecules
US20100316703A1 (en) * 2000-12-01 2010-12-16 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V. Rna interference mediating small rna molecules
US20110112283A1 (en) * 2000-12-01 2011-05-12 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V. Rna interference mediating small rna molecules
US8895718B2 (en) 2000-12-01 2014-11-25 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. RNA interference mediating small RNA molecules
US8445237B2 (en) 2000-12-01 2013-05-21 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. RNA interference mediating small RNA molecules
US8362231B2 (en) 2000-12-01 2013-01-29 Max-Planck-Gesellschaft zur Föderung der Wissenschaften E.V. RNA interference mediating small RNA molecules
US8895721B2 (en) 2000-12-01 2014-11-25 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. RNA interference mediating small RNA molecules
US20110027883A1 (en) * 2000-12-01 2011-02-03 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V. Rna interference mediating small rna molecules
US20110065109A1 (en) * 2000-12-01 2011-03-17 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V. Rna interference mediating small rna molecules
US20110065773A1 (en) * 2000-12-01 2011-03-17 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V. Rna interference mediating small rna molecules
US8853384B2 (en) 2000-12-01 2014-10-07 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. RNA interference mediating small RNA molecules
US8796016B2 (en) 2000-12-01 2014-08-05 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. RNA interference mediating small RNA molecules
US20110014123A1 (en) * 2000-12-01 2011-01-20 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V. RNA interference mediating small RNA molecules
US9587240B2 (en) 2001-01-09 2017-03-07 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of a target gene
US20050159381A1 (en) * 2001-05-18 2005-07-21 Sirna Therapeutics, Inc. RNA interference mediated inhibition of chromosome translocation gene expression using short interfering nucleic acid (siNA)
US20050176024A1 (en) * 2001-05-18 2005-08-11 Sirna Therapeutics, Inc. RNA interference mediated inhibition of epidermal growth factor receptor (EGFR) gene expression using short interfering nucleic acid (siNA)
US20050176025A1 (en) * 2001-05-18 2005-08-11 Sirna Therapeutics, Inc. RNA interference mediated inhibition of B-cell CLL/Lymphoma-2 (BCL-2) gene expression using short interfering nucleic acid (siNA)
US20050239731A1 (en) * 2001-05-18 2005-10-27 Sirna Therapeutics, Inc. RNA interference mediated inhibition of MAP kinase gene expression using short interfering nucleic acid (siNA)
US8772469B2 (en) * 2001-07-12 2014-07-08 Sanofi-Aventis Deutschland Gmbh Synthetic double-stranded oligonucleotides for specific inhibition of gene expression
US20030139585A1 (en) * 2001-07-12 2003-07-24 Eugen Uhlmann Synthetic double-stranded oligonucleotides for specific inhibition of gene expression
US10517887B2 (en) 2001-07-23 2019-12-31 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for RNAi mediated inhibition of gene expression in mammals
US20130066058A1 (en) * 2001-07-23 2013-03-14 John E. Thompson Use of Antisense Oligonucleotides or siRNA to Suppress Expression of eIF-5A1
US20090264505A1 (en) * 2001-07-23 2009-10-22 Kay Mark A Methods and compositions for rnai mediated inhibition of gene expression in mammals
US20110098460A1 (en) * 2001-07-23 2011-04-28 Senesco Technologies, Inc. SiRNA Useful to Suppress expression of eIF-5A1
US20030139363A1 (en) * 2001-07-23 2003-07-24 Kay Mark A. Methods and compositions for RNAi mediated inhibition of viral gene expression in mammals
US9018179B2 (en) 2001-07-23 2015-04-28 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for RNAi mediated inhibition of gene expression in mammals
US20100099740A1 (en) * 2001-07-23 2010-04-22 Kay Mark A Methods and compositions for rnai mediated inhibition of gene expression in mammals
US8242256B2 (en) * 2001-07-23 2012-08-14 Senesco Technologies, Inc. siRNA useful to supress expression of EIF-5A1
US20030153519A1 (en) * 2001-07-23 2003-08-14 Kay Mark A. Methods and compositions for RNAi mediated inhibition of gene expression in mammals
US10590418B2 (en) * 2001-07-23 2020-03-17 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for RNAi mediated inhibition of gene expression in mammals
US20090247606A1 (en) * 2001-08-28 2009-10-01 Sirna Therapeutics, Inc. RNA Interference Mediated Inhibition of Adenosine A1 Receptor (ADORA1) Gene Expression Using Short Interfering Nucleic Acid (siNA)
US20100305191A1 (en) * 2001-08-28 2010-12-02 Mcswiggen James Rna interference mediated inhibition of adenosine a1 receptor (adora1) gene expression using short interfering rna
US7691821B2 (en) 2001-09-19 2010-04-06 University Of South Florida Inhibition of SHIP to enhance stem cell harvest and transplantation
US20060223749A1 (en) * 2001-09-19 2006-10-05 University Of South Florida Inhibition of SHIP to enhance stem cell harvest and transplantation
US20050074757A1 (en) * 2001-10-12 2005-04-07 Ribopharma Ag Compositions and methods for inhibiting expression of a mutant gene
US7348314B2 (en) * 2001-10-12 2008-03-25 Alnylam Europe Ag Compositions and methods for inhibiting viral replication
US7763590B2 (en) * 2001-10-12 2010-07-27 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of a mutant gene
US7745418B2 (en) 2001-10-12 2010-06-29 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting viral replication
US20040091457A1 (en) * 2001-10-12 2004-05-13 Ribopharma Ag Compositions and methods for inhibiting viral replication
US20030157030A1 (en) * 2001-11-02 2003-08-21 Insert Therapeutics, Inc. Methods and compositions for therapeutic use of rna interference
US20040063654A1 (en) * 2001-11-02 2004-04-01 Davis Mark E. Methods and compositions for therapeutic use of RNA interference
US20090304798A1 (en) * 2001-11-02 2009-12-10 Insert Therapeutics, Inc. Methods and compositions for therapeutic use of RNA interference
US20050004064A1 (en) * 2001-11-21 2005-01-06 Mitsubishi Chemical Corporation Method of inhibiting gene expression
US20050148526A1 (en) * 2002-01-23 2005-07-07 Kisielow Malgorzata A. Methods of obtaining isoform specific expression in mammalian cells
US9592250B2 (en) 2002-02-01 2017-03-14 Life Technologies Corporation Double-stranded oligonucleotides
US9777275B2 (en) 2002-02-01 2017-10-03 Life Technologies Corporation Oligonucleotide compositions with enhanced efficiency
US10036025B2 (en) 2002-02-01 2018-07-31 Life Technologies Corporation Oligonucleotide compositions with enhanced efficiency
US20060009409A1 (en) * 2002-02-01 2006-01-12 Woolf Tod M Double-stranded oligonucleotides
US10626398B2 (en) 2002-02-01 2020-04-21 Life Technologies Corporation Oligonucleotide compositions with enhanced efficiency
US8815821B2 (en) 2002-02-01 2014-08-26 Life Technologies Corporation Double-stranded oligonucleotides
US10196640B1 (en) 2002-02-01 2019-02-05 Life Technologies Corporation Oligonucleotide compositions with enhanced efficiency
US9796978B1 (en) 2002-02-01 2017-10-24 Life Technologies Corporation Oligonucleotide compositions with enhanced efficiency
US20040054155A1 (en) * 2002-02-01 2004-03-18 Sequitur, Inc. Oligonucleotide compositions with enhanced efficiency
US20040014956A1 (en) * 2002-02-01 2004-01-22 Sequitur, Inc. Double-stranded oligonucleotides
US10106793B2 (en) 2002-02-01 2018-10-23 Life Technologies Corporation Double-stranded oligonucleotides
US20150099797A1 (en) * 2002-02-01 2015-04-09 Life Technologies Corporation Oligonucleotide compositions with enhanced efficiency
US8524680B2 (en) * 2002-02-01 2013-09-03 Applied Biosystems, Llc High potency siRNAS for reducing the expression of target genes
US20050096289A1 (en) * 2002-02-07 2005-05-05 Hans Prydz Methods and compositions for modulating tissue factor
US20090042826A1 (en) * 2002-02-12 2009-02-12 Quark Pharmaceuticals, Inc. Use of the AXL receptor for diagnosis and treatment of renal disease
US20070087991A1 (en) * 2002-02-13 2007-04-19 Axordia Limited Pluripotential stem cells
US7820632B2 (en) * 2002-02-14 2010-10-26 City Of Hope Methods for producing interfering RNA molecules in mammalian cells and therapeutic uses for such molecules
US20040096843A1 (en) * 2002-02-14 2004-05-20 Rossi John J. Methods for producing interfering RNA molecules in mammalian cells and therapeutic uses for such molecules
US8106181B2 (en) 2002-02-14 2012-01-31 City Of Hope Methods for producing interfering RNA molecules in mammalian cells and therapeutic uses for such molecules
US8076071B2 (en) 2002-02-14 2011-12-13 City Of Hope Methods for producing interfering RNA molecules in mammalian cells and therapeutic uses for such molecules
US9181584B2 (en) 2002-02-14 2015-11-10 City Of Hope Methods for producing interfering RNA molecules in mammalian cells and therapeutic uses for such molecules
US8927519B2 (en) 2002-02-14 2015-01-06 City Of Hope Methods for producing interfering RNA molecules in mammalian cells and therapeutic uses for such molecules
US20110003307A1 (en) * 2002-02-14 2011-01-06 City Of Hope Methods for producing interfering rna molecules in mammalian cells and therapeutic uses for such molecules
US8232383B2 (en) 2002-02-20 2012-07-31 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US20090093437A1 (en) * 2002-02-20 2009-04-09 Sirna Therapeutics, Inc. RNA INTERFERENCE MEDIATED INHIBITION OF CHECKPOINT KINASE-1 (CHK-1) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
US20090093435A1 (en) * 2002-02-20 2009-04-09 Sirna Therapeutics, Inc. RNA INTERFERENCE MEDIATED INHIBITION OF GRB2 ASSOCIATED BINDING PROTEIN (GAB2) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
US20060293271A1 (en) * 2002-02-20 2006-12-28 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US20090023675A1 (en) * 2002-02-20 2009-01-22 Sirna Therapeutics, Inc. RNA Interference Mediated Inhibition of Gene Expression Using Chemically Modified Short Interfering Nucleic Acid (siNA)
US20090093439A1 (en) * 2002-02-20 2009-04-09 Sirna Therapeutics, Inc. RNA INTERFERENCE MEDIATED INHIBITION OF CHROMOSOME TRANSLOCATION GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
US20090099116A1 (en) * 2002-02-20 2009-04-16 Sirna Therapeutics, Inc. RNA INTERFERENCE MEDIATED INHIBITION OF FOS GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
US8648185B2 (en) 2002-02-20 2014-02-11 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US8202979B2 (en) 2002-02-20 2012-06-19 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid
US20060281175A1 (en) * 2002-02-20 2006-12-14 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US20100240730A1 (en) * 2002-02-20 2010-09-23 Merck Sharp And Dohme Corp. RNA Interference Mediated Inhibition of Gene Expression Using Chemically Modified Short Interfering Nucleic Acid (siNA)
US20100228018A1 (en) * 2002-02-20 2010-09-09 Mcswiggen James RNA INTERFERENCE MEDIATED INHIBITION OF PROLIFERATING CELL NUCLEAR ANTIGEN (PCNA) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
US20070167393A1 (en) * 2002-02-20 2007-07-19 Sirna Therapeutics, Inc. RNA INTERFERENCE MEDIATED INHIBITION OF GENE EXPRESSION USING CHEMICALLY MODIFIED SHORT INTERFERING NUCLEIC ACID (siNA)
US20100227911A1 (en) * 2002-02-20 2010-09-09 Mcswiggen James RNA INTERFERENCE MEDIATED INHIBITION OF CHROMOSOME TRANSLOCATION GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
US10662428B2 (en) 2002-02-20 2020-05-26 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US9738899B2 (en) 2002-02-20 2017-08-22 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US8846894B2 (en) 2002-02-20 2014-09-30 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US9732344B2 (en) 2002-02-20 2017-08-15 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US20090137509A1 (en) * 2002-02-20 2009-05-28 Sirna Therapeutics, Inc. RNA INTERFERENCE MEDIATED INHIBITION OF PROLIFERATION CELL NUCLEAR ANTIGEN (PCNA) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
US20090137508A1 (en) * 2002-02-20 2009-05-28 Sirna Therapeutics, Inc. RNA INTERFERENCE MEDIATED INHIBITION OF POLYCOMB GROUP PROTEIN EZH2 GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
US9657294B2 (en) 2002-02-20 2017-05-23 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US8618277B2 (en) 2002-02-20 2013-12-31 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US20100184825A1 (en) * 2002-02-20 2010-07-22 Merck Sharp & Dohme Corp. RNA Interference Mediated Inhibition of Protein Tyrosine Phosphatase-1B (PTP-1B) Gene Expression Using Short Interfering Nucleic Acid (siNA)
US20040192626A1 (en) * 2002-02-20 2004-09-30 Mcswiggen James RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US9957517B2 (en) 2002-02-20 2018-05-01 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US20100184824A1 (en) * 2002-02-20 2010-07-22 Sirna Therapeutics, Inc. RNA Interference Mediated Inhibition of Interleukin and Interleukin Receptor Gene Expression Using Short Interfering Nucleic Acid (siNA)
US10000754B2 (en) 2002-02-20 2018-06-19 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US7897755B2 (en) * 2002-02-20 2011-03-01 Sirna Therapeutics, Inc. RNA interference mediated inhibition of platelet-derived endothelial cell growth factor (ECGF1) gene expression using short interfering nucleic acid (siNA)
US7897756B2 (en) * 2002-02-20 2011-03-01 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of NOGO and NOGO receptor gene expression using short interfering nucleic acid (siNA)
US20040219671A1 (en) * 2002-02-20 2004-11-04 Sirna Therapeutics, Inc. RNA interference mediated treatment of parkinson disease using short interfering nucleic acid (siNA)
US10889815B2 (en) 2002-02-20 2021-01-12 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US7855284B2 (en) 2002-02-20 2010-12-21 Sirna Therapeutics, Inc. RNA interference mediated inhibition of checkpoint kinase-1 (CHK-1) gene expression using short interfering nucleic acid (siNA)
US7897757B2 (en) * 2002-02-20 2011-03-01 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of protein tyrosine phosphatase-1B (PTP-1B) gene expression using short interfering nucleic acid (siNA)
US20090233983A1 (en) * 2002-02-20 2009-09-17 Sirna Therapeutics Inc. RNA Interference Mediated Inhibition of Protein Tyrosine Phosphatase-1B (PTP-1B) Gene Expression Using Short Interfering RNA
US20090247613A1 (en) * 2002-02-20 2009-10-01 Sirna Therapeutics, Inc. RNA INTERFERENCE MEDIATED INHIBITION OF B-CELL CLL/LYMPHOMA-2 (BCL2) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
US20090253772A1 (en) * 2002-02-20 2009-10-08 Sirna Therapeutics, Inc. RNA INTERFERENCE MEDIATED INHIBITION OF CXCR4 GENE EXPRESSION USING SHORT INTERFERING NUCELEIC ACID (siNA)
US20100144842A1 (en) * 2002-02-20 2010-06-10 Sirna Therapeutics, Inc. RNA Interference Mediated Inhibition of NOGO and NOGO Receptor Gene Expression Using Short Interfering Nucleic Acid (siNA)
US7910724B2 (en) * 2002-02-20 2011-03-22 Sirna Therapeutics, Inc. RNA interference mediated inhibition of Fos gene expression using short interfering nucleic acid (siNA)
US20100144851A1 (en) * 2002-02-20 2010-06-10 Sirna Therapeutics, Inc. RNA Interference Mediated Inhibition of Platelet-Derived Endothelial Cell Growth Factor (ECGF1) Gene Expression Using Short Interfering Nucleic Acid (siNA)
US20050142578A1 (en) * 2002-02-20 2005-06-30 Sirn Therapeutics, Inc. RNA interference mediated target discovery and target validation using short interfering nucleic acid (siNA)
US20100130592A1 (en) * 2002-02-20 2010-05-27 Sirna Therapeutics, Inc. RNA Interference Mediated Inhibition of GRB2 Associated Binding Protein (GAB2) Gene Expression Using Short Interfering Nucleic Acid (siNA)
US7910725B2 (en) * 2002-02-20 2011-03-22 Sirna Therapeutics, Inc. RNA interference mediated inhibition of interleukin and interleukin receptor gene expression using short interfering nucleic acid (siNA)
US9771588B2 (en) 2002-02-20 2017-09-26 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US7923549B2 (en) * 2002-02-20 2011-04-12 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of interleukin and interleukin receptor gene expression using short interfering nucleic acid (siNA)
US7928218B2 (en) 2002-02-20 2011-04-19 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of polycomb group protein EZH2 gene expression using short interfering nucleic acid (siNA)
US20120004403A1 (en) * 2002-02-20 2012-01-05 Leonid Beigelman RNA INTERFERENCE MEDIATED INHIBITION OF TNF AND TNF RECEPTOR GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
US8013143B2 (en) * 2002-02-20 2011-09-06 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of CXCR4 gene expression using short interfering nucleic acid (siNA)
US20100113564A1 (en) * 2002-02-20 2010-05-06 Mcswiggen James RNA Interference Mediated Inhibition of Interleukin and Interleukin Receptor Gene Expression Using Short Interfering Nucleic Acid (siNA)
US20090306182A1 (en) * 2002-02-20 2009-12-10 Sirna Therapeutics, Inc. RNA INTERFERENCE MEDIATED INHIBITION OF MAP KINASE GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
US7956178B2 (en) * 2002-02-20 2011-06-07 Mcswiggen James RNA interference mediated inhibition of GRB2 associated binding protein (GAB2) gene expression using short interfering nucleic acid (siNA)
US9181551B2 (en) 2002-02-20 2015-11-10 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US7989612B2 (en) 2002-02-20 2011-08-02 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US7667029B2 (en) * 2002-02-20 2010-02-23 Sirna Therapeutics, Inc. RNA interference mediated inhibition of checkpoint kinase-1 (CHK-1) gene expression using short interfering nucleic acid (siNA)
US20100099743A1 (en) * 2002-02-20 2010-04-22 Sirna Therapeutics, Inc. RNA INTERFERENCE MEDIATED INHIBITION OF CHECKPOINT KINASE-1 (CHK-1) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
US10351852B2 (en) 2002-02-20 2019-07-16 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US7662952B2 (en) * 2002-02-20 2010-02-16 Sirna Therapeutics, Inc. RNA interference mediated inhibition of GRB2 associated binding protein (GAB2) gene expression using short interfering nucleic acid (siNA)
US20040005593A1 (en) * 2002-03-06 2004-01-08 Rigel Pharmaceuticals, Inc. Novel method for delivery and intracellular synthesis of siRNA molecules
US20040248296A1 (en) * 2002-03-20 2004-12-09 Beresford Paul J. HIV therapeutic
US8946180B2 (en) 2002-04-18 2015-02-03 Opko Pharmaceuticals, Llc Means and methods for the specific modulation of target genes in the CNS and the eye and methods for their identification
US20060003915A1 (en) * 2002-04-18 2006-01-05 Karina Drumm Means and methods for the specific modulation of target genes in the cns and the eye and methods for their identification
US20110021605A1 (en) * 2002-04-18 2011-01-27 Schulte Ralf Wilhelm Means and methods for the specific inhibition of genes in cells and tissue of the cns and/or eye
US8202845B2 (en) 2002-04-18 2012-06-19 Acuity Pharmaceuticals, Inc. Means and methods for the specific modulation of target genes in the CNS and the eye and methods for their identification
US9856476B2 (en) 2002-05-03 2018-01-02 Duke University Method of regulating gene expression
US10233451B2 (en) 2002-05-03 2019-03-19 Duke University Method of regulating gene expression
US9850485B2 (en) 2002-05-03 2017-12-26 Duke University Method of regulating gene expression
US8409796B2 (en) 2002-05-03 2013-04-02 Duke University Method of regulating gene expression
US8137910B2 (en) 2002-05-03 2012-03-20 Duke University Method of regulating gene expression
US9267145B2 (en) 2002-05-03 2016-02-23 Duke University Method of regulating gene expression
US20040053411A1 (en) * 2002-05-03 2004-03-18 Duke University Method of regulating gene expression
US8865884B2 (en) * 2002-05-23 2014-10-21 Isis Pharmaceuticals, Inc. Antisense modulation of kinesin-like 1 expression
US20130331435A1 (en) * 2002-05-23 2013-12-12 Isis Pharmaceuticals, Inc. Antisense modulation of kinesin-like 1 expression
US7704965B2 (en) * 2002-06-26 2010-04-27 The Penn State Research Foundation Methods and materials for treating human papillomavirus infections
US20060058252A1 (en) * 2002-06-26 2006-03-16 Clawson Gary A Methods and materials for treating human papillomavirus infections
US9504651B2 (en) 2002-06-28 2016-11-29 Protiva Biotherapeutics, Inc. Lipid compositions for nucleic acid delivery
US11298320B2 (en) 2002-06-28 2022-04-12 Arbutus Biopharma Corporation Liposomal apparatus and manufacturing methods
US11318098B2 (en) 2002-06-28 2022-05-03 Arbutus Biopharma Corporation Liposomal apparatus and manufacturing methods
US9492386B2 (en) 2002-06-28 2016-11-15 Protiva Biotherapeutics, Inc. Liposomal apparatus and manufacturing methods
US8546345B2 (en) 2002-07-24 2013-10-01 The Trustees Of The University Of Pennsylvania Compositions and methods for siRNA inhibition of angiogenesis
US8946403B2 (en) 2002-07-24 2015-02-03 The Trustees Of The University Of Pennsylvania Compositions and methods for siRNA inhibition of angiogenesis
US9150863B2 (en) 2002-07-24 2015-10-06 The Trustees Of The University Of Pennsylvania Compositions and methods for siRNA inhibition of angiogenesis
US8541384B2 (en) 2002-07-24 2013-09-24 The Trustees Of The University Of Pennsylvania Compositions and methods for siRNA inhibition of angiogenesis
US20070037762A1 (en) * 2002-07-24 2007-02-15 Tolentino Michael J COMPOSITIONS AND METHODS FOR siRNA INHIBITION OF ANGIOGENESIS
US20060293256A1 (en) * 2002-08-06 2006-12-28 Masateru Yamada Remedy or preventive for kidney disease and method of diagnosing kidney disease
US20060211637A1 (en) * 2002-08-06 2006-09-21 Intradigm Corporation Methods of down regulating target gene expression in vivo by introduction of interfering rna
US20080269155A1 (en) * 2002-08-06 2008-10-30 Toray Industries Inc. Remedy or preventive for kidney disease and method of diagnosing kidney disease
US20040203145A1 (en) * 2002-08-07 2004-10-14 University Of Massachusetts Compositions for RNA interference and methods of use thereof
US8729036B2 (en) 2002-08-07 2014-05-20 University Of Massachusetts Compositions for RNA interference and methods of use thereof
US9611472B2 (en) 2002-08-07 2017-04-04 University Of Massachusetts Compositions for RNA interference and methods of use thereof
US20110009472A1 (en) * 2002-08-21 2011-01-13 The University Of British Columbia RNAi Probes Targeting Cancer-Related Proteins
US20080274996A1 (en) * 2002-08-21 2008-11-06 The University Of British Columbia RNAi Probes Targeting Cancer-Related Proteins
US7820635B2 (en) 2002-08-21 2010-10-26 The University Of British Columbia RNAi probes targeting cancer-related proteins
US20060178329A1 (en) * 2002-08-21 2006-08-10 The University Of British Columbia RNAi probes targeting cancer-related proteins
US9487777B2 (en) 2002-08-21 2016-11-08 The University Of British Columbia RNAi probes targeting cancer-related proteins
US7964717B2 (en) * 2002-08-21 2011-06-21 The University Of British Columbia RNAi probes targeting cancer-related proteins
US20040096882A1 (en) * 2002-08-21 2004-05-20 Martin Gleave RNAi probes targeting cancer-related proteins
US8252918B2 (en) * 2002-08-21 2012-08-28 The University Of British Columbia RNAi probes targeting cancer-related proteins
US8759308B2 (en) 2002-08-21 2014-06-24 The University Of British Columbia RNAi probes targeting cancer-related proteins
US20060276635A1 (en) * 2002-09-05 2006-12-07 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US7923547B2 (en) * 2002-09-05 2011-04-12 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US20070004664A1 (en) * 2002-09-05 2007-01-04 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US7956176B2 (en) 2002-09-05 2011-06-07 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US20060287269A1 (en) * 2002-09-09 2006-12-21 The Regents Of The University Of California Short interfering nucleic acid hybrids and methods thereof
US7655634B2 (en) * 2002-09-18 2010-02-02 The Burnham Institute Use of hepatitis B X-interacting protein (HBXIP) in modulation of apoptosis
US20100190195A1 (en) * 2002-09-18 2010-07-29 The Burnham Institute Use of Hepatitis B X-Interacting Protein (HBXIP) in Modulation of Apoptosis
US20070270369A1 (en) * 2002-09-18 2007-11-22 The Burnham Institute Use of hepatitis b x-interacting protein (hbxip) in modulation of apoptosis
US20060257380A1 (en) * 2002-09-19 2006-11-16 Inst.Nat. De La Sante Et De La Recherche MED Use of sirnas for gene silencing in antigen presenting cells
US20090106852A1 (en) * 2002-09-28 2009-04-23 Massachusetts Institute Of Technology Influenza Therapeutic
US20090124567A1 (en) * 2002-09-28 2009-05-14 Jianzhu Chen Influenza Therapeutic
US20040242518A1 (en) * 2002-09-28 2004-12-02 Massachusetts Institute Of Technology Influenza therapeutic
US20060240425A1 (en) * 2002-09-30 2006-10-26 Oncotherapy Science, Inc Genes and polypeptides relating to myeloid leukemia
US7422853B1 (en) * 2002-10-04 2008-09-09 Myriad Genetics, Inc. RNA interference using a universal target
US20090048111A1 (en) * 2002-10-04 2009-02-19 Myriad Genetics, Incorporated Rna interference using a universal target
US7521431B2 (en) * 2002-11-01 2009-04-21 The Trustees Of The University Of Pennsylvania Compositions and methods for siRNA inhibition of HIF-1 alpha
US8236775B2 (en) 2002-11-01 2012-08-07 The Trustees Of The University Of Pennsylvania Compositions and methods for siRNA inhibition of HIF-1 α
US20100136101A1 (en) * 2002-11-01 2010-06-03 The Trustees Of The University Of Pennsylvania Compositions and methods for sirna inhibition of hif-1 alpha
US20040180357A1 (en) * 2002-11-01 2004-09-16 The Trustees Of The University Of Pennsylvania Compositions and methods for siRNA inhibition of HIF-1 alpha
US7645744B2 (en) 2002-11-01 2010-01-12 The Trustees Of The University Of Pennsylvania Compositions and methods for siRNA inhibition of HIF-1 alpha
US7892793B2 (en) 2002-11-04 2011-02-22 University Of Massachusetts Allele-specific RNA interference
US20060128650A1 (en) * 2002-11-04 2006-06-15 University Of Massachusetts Allele-specific RNA interference
US8604183B2 (en) 2002-11-05 2013-12-10 Isis Pharmaceuticals, Inc. Compositions comprising alternating 2′-modified nucleosides for use in gene modulation
US20080076908A1 (en) * 2002-11-14 2008-03-27 Dharmacon, Inc. siRNA targeting nuclear receptors
US10765695B2 (en) 2002-11-14 2020-09-08 Thermo Fisher Scientific Inc. Methods and compositions for selecting siRNA of improved functionality
US8222395B2 (en) 2002-11-14 2012-07-17 Dharmacon, Inc. siRNA targeting kinase insert domain receptor (KDR)
US9771586B2 (en) 2002-11-14 2017-09-26 Thermo Fisher Scientific Inc. RNAi targeting ZNF205
US20090082556A1 (en) * 2002-11-14 2009-03-26 Dharmacon, Inc. siRNA targeting TATA box binding protein (TBP)-associated factor (TAF1)
US8232385B2 (en) 2002-11-14 2012-07-31 Dharmacon, Inc. siRNA targeting cyclin-dependent kinase inhibitor 1B (p27, Kip1) (CDKN1B)
US7511132B2 (en) * 2002-11-14 2009-03-31 Dharmacon, Inc. Functional and hyperfunctional siRNA directed against Bcl-2
US20090088563A1 (en) * 2002-11-14 2009-04-02 Dharmacon, Inc. siRNA targeting Transducin (beta)-like 3 (TBL3)
US7514550B2 (en) * 2002-11-14 2009-04-07 Dharmacon, Inc. siRNA targeting myeloid cell leukemia sequence 1
US9777270B2 (en) 2002-11-14 2017-10-03 Thermo Fisher Scientific Inc. Methods and compositions for selecting siRNA of improved functionality
US9839649B2 (en) 2002-11-14 2017-12-12 Thermo Fisher Scientific Inc. Methods and compositions for selecting siRNA of improved functionality
US8217162B2 (en) 2002-11-14 2012-07-10 Dharmacon, Inc. siRNA targeting interleukin-1 receptor-associated kinase 4(IRAK4)
US20090005548A1 (en) * 2002-11-14 2009-01-01 Dharmacon, Inc. siRNA targeting nuclear receptor interacting protein 1 (NRIP1)
US8198427B1 (en) 2002-11-14 2012-06-12 Dharmacon, Inc. SiRNA targeting catenin, beta-1 (CTNNB1)
US8232386B2 (en) 2002-11-14 2012-07-31 Dharmacon, Inc. SiRNA targeting apolipoprotein B (APOB)
US8236942B2 (en) 2002-11-14 2012-08-07 Dharmacon, Inc. SiRNA targeting glucagon receptor (GCGR)
US8247169B2 (en) 2002-11-14 2012-08-21 Dharmacon, Inc. SiRNA targeting diacylglycerol O-acyltransferase homolog 2 (DGAT2)
US8138329B2 (en) 2002-11-14 2012-03-20 Dharmacon, Inc. siRNA targeting connective tissue growth factor (CTGF)
US20120010106A1 (en) * 2002-11-14 2012-01-12 Dharmacon, Inc. siRNA targeting myeloid differentiation primary response gene (88) (MYD88)
US8093370B2 (en) 2002-11-14 2012-01-10 Dharmacon, Inc. siRNA targeting spleen tyrosine kinase
US8090542B2 (en) 2002-11-14 2012-01-03 Dharmacon Inc. Functional and hyperfunctional siRNA
US20050246794A1 (en) * 2002-11-14 2005-11-03 Dharmacon Inc. Functional and hyperfunctional siRNA
US8293887B2 (en) 2002-11-14 2012-10-23 Dharmacon, Inc. SiRNA targeting beta secretase (BACE)
US20050245475A1 (en) * 2002-11-14 2005-11-03 Dharmacon, Inc. Functional and hyperfunctional siRNA directed against Bcl-2
US20080319180A1 (en) * 2002-11-14 2008-12-25 Dharmacon, Inc. siRNA targeting protein kinase N-3 (PKN-3)
US8071754B2 (en) 2002-11-14 2011-12-06 Dharmacon, Inc. siRNA targeting apolipoprotein B (APOB)
US20090118489A1 (en) * 2002-11-14 2009-05-07 Dharmacon, Inc. siRNA targeting nucleoporin 62kDa (Nup62)
US20080306015A1 (en) * 2002-11-14 2008-12-11 Dharmacon, Inc. siRNA targeting proprotein convertase subtilisin/kexin type 9 (PCSK9)
US8067576B2 (en) 2002-11-14 2011-11-29 Dharmacon, Inc. siRNA targeting serine/threonine kinase 12 (STK12 or aurora B kinase)
US8304528B2 (en) 2002-11-14 2012-11-06 Dharmacon, Inc. SiRNA targeting fructose-1, 6-bisphosphatase 1 (FBP1)
US9719094B2 (en) 2002-11-14 2017-08-01 Thermo Fisher Scientific Inc. RNAi targeting SEC61G
US20080293593A1 (en) * 2002-11-14 2008-11-27 Dharmacon, Inc. siRNA targeting casitas B cell lymphoma-B (CBL-B)
US20080293595A1 (en) * 2002-11-14 2008-11-27 Dharmacon, Inc. siRNA targeting protein tyrosine phosphatase-1B (PTP1B)
US8039610B2 (en) 2002-11-14 2011-10-18 Dharmacon, Inc. siRNA targeting superoxide dismutase 1 (SOD1)
US9719092B2 (en) 2002-11-14 2017-08-01 Thermo Fisher Scientific Inc. RNAi targeting CNTD2
US8314229B2 (en) 2002-11-14 2012-11-20 Dharmacon, Inc. siRNA targeting tie-2
US8030476B2 (en) 2002-11-14 2011-10-04 Dharmacon, Inc. siRNA targeting gremlin
US9879266B2 (en) 2002-11-14 2018-01-30 Thermo Fisher Scientific Inc. Methods and compositions for selecting siRNA of improved functionality
US20090149644A1 (en) * 2002-11-14 2009-06-11 Dharmacon Inc. siRNA Targeting KRAS
US20080268457A1 (en) * 2002-11-14 2008-10-30 Dharmacon, Inc. siRNA targeting forkhead box P3 (FOXP3)
US20090156797A1 (en) * 2002-11-14 2009-06-18 Dharmacon, Inc. siRNA Targeting Hypoxia-inducible Factor 1
US8030474B2 (en) 2002-11-14 2011-10-04 Dharmacon, Inc. siRNA targeting cyclin-dependent kinase 4 (CDK4)
US7550572B2 (en) * 2002-11-14 2009-06-23 Dharmacon, Inc. siRNA targeting cell division cycle 20 homolog (CDC20)
US20090163701A1 (en) * 2002-11-14 2009-06-25 Dharmacon Inc. siRNA targeting tumor necrosis factor receptor superfamily member 1A
US20050255487A1 (en) * 2002-11-14 2005-11-17 Dharmacon, Inc. Methods and compositions for selecting siRNA of improved functionality
US20090163702A1 (en) * 2002-11-14 2009-06-25 Dharmacon Inc. siRNA targeting Myeloid cell leukemia sequence 1
US8022198B2 (en) 2002-11-14 2011-09-20 Dharmacon, Inc. siRNA targeting histamine receptor H1
US8022199B2 (en) 2002-11-14 2011-09-20 Dharmacon, Inc. SiRNA targeting myeloid differentiation primary response gene (88) (MYD88)
US20090191625A1 (en) * 2002-11-14 2009-07-30 Dharmacon, Inc. siRNA targeting connective tissue growth factor (CTGF)
US8013145B2 (en) 2002-11-14 2011-09-06 Dharmacon, Inc. SiRNA targeting cyclin-dependent kinase inhibitor 1B (p27, Kip1) (CDKN1B)
US8008474B2 (en) 2002-11-14 2011-08-30 Dharmacon, Inc. siRNA targeting KRAS
US10011836B2 (en) 2002-11-14 2018-07-03 Thermo Fisher Scientific Inc. Methods and compositions for selecting siRNA of improved functionality
US7999097B2 (en) 2002-11-14 2011-08-16 Dharmacon, Inc. siRNA targeting beta secretase (BACE)
US8000902B2 (en) 2002-11-14 2011-08-16 Dharmacon, Inc. Methods and compositions for selecting siRNA of improved functionality
US7985854B2 (en) 2002-11-14 2011-07-26 Dharmacon, Inc. siRNA targeting TATA box binding protein (TBP)-associated factor (TAF1)
US7576196B2 (en) 2002-11-14 2009-08-18 Dharmacon, Inc. siRNA targeting transducin (beta)-like 3 (TBL3)
US7576197B2 (en) 2002-11-14 2009-08-18 Dharmacon, Inc. SiRNA targeting KRAS
US7977471B2 (en) 2002-11-14 2011-07-12 Dharmacon, Inc. siRNA targeting TNFα
US7582746B2 (en) 2002-11-14 2009-09-01 Dharmacon, Inc. siRNA targeting complement component 3 (C3)
US8426579B2 (en) * 2002-11-14 2013-04-23 Dharmacon, Inc. SiRNA targeting myeloid differentiation primary response gene (88) (MYD88)
US20090227780A1 (en) * 2002-11-14 2009-09-10 Dharmacon, Inc. siRNA targeting connexin 43
US7589191B2 (en) 2002-11-14 2009-09-15 Dharmacon, Inc. siRNA targeting hypoxia-inducible factor 1
US20080227967A1 (en) * 2002-11-14 2008-09-18 Dharmacon, Inc. siRNA targeting ribonucleotide reductase M2 polypeptide (RRM2 or RNR-R2)
US8461326B2 (en) 2002-11-14 2013-06-11 Dharmacon, Inc. SiRNA targeting connective tissue growth factor (CTGF)
US20130210676A1 (en) * 2002-11-14 2013-08-15 Dharmacon, Inc. siRNA Targeting Myeloid Differentiation Primary Response Gene (88) (MYD88)
US7592443B2 (en) 2002-11-14 2009-09-22 Dharmacon, Inc. siRNA targeting interleukin-1 receptor-associated kinase 4 (IRAK4)
US7592444B2 (en) 2002-11-14 2009-09-22 Dharmacon, Inc. siRNA targeting myeloid cell leukemia sequence 1
US7592442B2 (en) 2002-11-14 2009-09-22 Dharmacon, Inc. siRNA targeting ribonucleotide reductase M2 polypeptide (RRM2 or RNR-R2)
US7951935B2 (en) 2002-11-14 2011-05-31 Dharmacon, Inc. siRNA targeting v-myc myelocytomatosis viral oncogene homolog (MYC)
US20110105363A1 (en) * 2002-11-14 2011-05-05 Dharmacon, Inc. siRNA targeting TNFa
US20080177051A1 (en) * 2002-11-14 2008-07-24 Dharmacon, Inc. siRNA targeting cyclin-dependent kinase inhibitor 1B (p27, Kip1) (CDKN1B)
US7935813B2 (en) 2002-11-14 2011-05-03 Dharmacon, Inc. siRNA target hypoxia-inducible factor 1
US7595389B2 (en) 2002-11-14 2009-09-29 Dharmacon, Inc. siRNA targeting casitas B cell lymphoma-B (CBL-B)
US7897754B2 (en) 2002-11-14 2011-03-01 Dharmacon, Inc. SiRNA targeting ras-related nuclear protein RAN
US7893247B2 (en) 2002-11-14 2011-02-22 Dharmacon, Inc. siRNA targeting spleen tyrosine kinase
US8633306B2 (en) 2002-11-14 2014-01-21 Thermo Fisher Scientific Biosciences Inc. SiRNA targeting histamine receptor H1
US20070031844A1 (en) * 2002-11-14 2007-02-08 Anastasia Khvorova Functional and hyperfunctional siRNA
US20100323922A1 (en) * 2002-11-14 2010-12-23 Dharmacon, Inc. siRNA targeting TATA box binding protein (TBP)-associated factor (TAF1)
US7598369B2 (en) 2002-11-14 2009-10-06 Dharmacon, Inc. siRNA targeting histamine receptor H1
US7598370B2 (en) 2002-11-14 2009-10-06 Dharmacon, Inc. siRNA targeting polo-like kinase-1 (PLK-1)
US7855186B2 (en) 2002-11-14 2010-12-21 Dharmacon, Inc. siRNA targeting TIE-2
US20090253776A1 (en) * 2002-11-14 2009-10-08 Dharmacon, Inc. siRNA targeting gremlin
US20070072823A1 (en) * 2002-11-14 2007-03-29 Dharmacon Inc. siRNA targeting survivin
US20070088154A1 (en) * 2002-11-14 2007-04-19 Dharmacon Inc. siRNA targeting complement factor B
US7605252B2 (en) * 2002-11-14 2009-10-20 Dharmacon, Inc. siRNA targeting kinase insert domain receptor (KDR)
US11198870B2 (en) 2002-11-14 2021-12-14 Thermo Fisher Scientific Inc. Methods and compositions for selecting siRNA of improved functionality
US20070128640A1 (en) * 2002-11-14 2007-06-07 Dharmacon, Inc. siRNA targeting ras-related nuclear protein
US10233449B2 (en) 2002-11-14 2019-03-19 Thermo Fisher Scientific Inc. Methods and compositions for selecting siRNA of improved functionality
US7608706B2 (en) * 2002-11-14 2009-10-27 Dharmacon, Inc. siRNA targeting ras-related nuclear protein
US7608707B2 (en) * 2002-11-14 2009-10-27 Dharmacon, Inc. siRNA targeting survivin
US7612196B2 (en) 2002-11-14 2009-11-03 Dharmacon, Inc. siRNA targeting cyclin-dependent kinase inhibitor 1B (p27, Kip1) (CDKN1B)
US20070134698A1 (en) * 2002-11-14 2007-06-14 Dharmacon, Inc. siRNA targeting histamine receptor H1
US9228186B2 (en) 2002-11-14 2016-01-05 Thermo Fisher Scientific Inc. Methods and compositions for selecting siRNA of improved functionality
US7833989B2 (en) 2002-11-14 2010-11-16 Dharmacon, Inc. siRNA targeting connective tissue growth factor (CTGF)
US7615541B2 (en) 2002-11-14 2009-11-10 Dharmacon, Inc. siRNA targeting TIE-2
US7834170B2 (en) 2002-11-14 2010-11-16 Dharmacon, Inc. Functional and hyperfunctional siRNA
US7619081B2 (en) * 2002-11-14 2009-11-17 Dharmacon, Inc. siRNA targeting coatomer protein complex, subunit beta 2 (COPB2)
US20070134697A1 (en) * 2002-11-14 2007-06-14 Dharmacon, Inc. siRNA targeting TIE-2
US20080132691A1 (en) * 2002-11-14 2008-06-05 Dharmacon, Inc. siRNA targeting kinase insert domain receptor (KDR)
US7829696B2 (en) 2002-11-14 2010-11-09 Dharmacon, Inc. siRNA targeting amyloid beta (A4) precursor protein (APP)
US20070141602A1 (en) * 2002-11-14 2007-06-21 Dharmacon, Inc. siRNA targeting aquaporin 4
US20080113371A1 (en) * 2002-11-14 2008-05-15 Dharmacon, Inc. siRNA targeting beta secretase (BACE)
US20080113373A1 (en) * 2002-11-14 2008-05-15 Dharmacon, Inc. siRNA targeting amyloid beta (A4) precursor protein (APP)
US20090306356A1 (en) * 2002-11-14 2009-12-10 Dharmacon,Inc. siRNA Targeting TNFalpha
US7632939B2 (en) 2002-11-14 2009-12-15 Dharmacon, Inc. siRNA targeting proto-oncogene MET
US7820809B2 (en) 2002-11-14 2010-10-26 Dharmacon, Inc. Functional and hyperfunctional siRNA directed against Bcl-2
US7632938B2 (en) 2002-11-14 2009-12-15 Dharmacon, Inc. siRNA targeting superoxide dismutase 1 (SOD1)
US7635771B2 (en) 2002-11-14 2009-12-22 Dharmacon, Inc. siRNA targeting amyloid beta (A4) precursor protein (APP)
US7635770B2 (en) 2002-11-14 2009-12-22 Dharmacon, Inc. siRNA targeting protein kinase N-3 (PKN-3)
US7816512B2 (en) 2002-11-14 2010-10-19 Dharmacon, Inc. siRNA targeting proto-oncogene MET
US20090325818A1 (en) * 2002-11-14 2009-12-31 Dharmacon, Inc. siRNA targeting interleukin-1 receptor-associated kinase 4 (IRAK4)
US7807819B2 (en) 2002-11-14 2010-10-05 Dharmacon, Inc. siRNA targeting survivin
US7642349B2 (en) 2002-11-14 2010-01-05 Dharmacon, Inc. siRNA targeting TATA box binding protein (TBP)-associated factor (TAF1)
US20100248990A1 (en) * 2002-11-14 2010-09-30 Dharmacon, Inc. siRNA targeting ribonucleotide reductase M2 polypeptide (RRM2 or RNR-R2)
US7803933B2 (en) 2002-11-14 2010-09-28 Dharmacon, Inc. siRNA targeting TATA box binding protein (TBP)-associated factor (TAF1)
US7795420B2 (en) 2002-11-14 2010-09-14 Dharmacon, Inc. Functional and hyperfunctional siRNA directed against Bcl-2
US20080113376A1 (en) * 2002-11-14 2008-05-15 Dharmacon, Inc. siRNA targeting myeloid differentiation primary response gene (88) (MYD88)
US20080113378A1 (en) * 2002-11-14 2008-05-15 Dharmacon, Inc. siRNA targeting interleukin-1 receptor-associated kinase 4 (IRAK4)
US20100016176A1 (en) * 2002-11-14 2010-01-21 Dharmacon. Inc. siRNA targeting histamine receptor H1
US7795421B2 (en) 2002-11-14 2010-09-14 Dharmacon, Inc. siRNA targeting apolipoprotein B (APOB)
US20080113375A1 (en) * 2002-11-14 2008-05-15 Dharmacon, Inc. siRNA targeting superoxide dismutase 1 (SOD1)
US10696968B2 (en) 2002-11-14 2020-06-30 Thermo Fisher Scientific Inc. Methods and compositions for selecting siRNA of improved functionality
US20070185320A1 (en) * 2002-11-14 2007-08-09 Dharmacon, Inc. siRNA targeting cell division cycle 20 homolog (CDC20)
US20100022413A1 (en) * 2002-11-14 2010-01-28 Dharmacon, Inc. siRNA targeting Ras-related nuclear protein RAN
US20100022763A1 (en) * 2002-11-14 2010-01-28 Dharmacon, Inc. siRNA targeting kinase insert domain receptor (KDR)
US20080113370A1 (en) * 2002-11-14 2008-05-15 Dharmacon, Inc. siRNA targeting apolipoprotein B (APOB)
US7655789B2 (en) 2002-11-14 2010-02-02 Dharmacon, Inc. siRNA targeting transient receptor potential cation channel, subfamily V, member 1 (TRPV1)
US7781575B2 (en) 2002-11-14 2010-08-24 Dharmacon, Inc. siRNA targeting tumor protein 53 (p53)
US20080114162A1 (en) * 2002-11-14 2008-05-15 Dharmacon Inc. Functional and hyperfunctional siRNA directed against Bcl-2
US7662950B2 (en) * 2002-11-14 2010-02-16 Dharmacon, Inc. siRNA targeting myeloid differentiation primary response gene (88) (MYD88)
US20080113372A1 (en) * 2002-11-14 2008-05-15 Dharmacon, Inc. siRNA targeting glucagon receptor (GCGR)
US20080113369A1 (en) * 2002-11-14 2008-05-15 Dharmacon, Inc. siRNA targeting diacylglycerol O-acyltransferase homolog 2 (DGAT2)
US7666853B2 (en) 2002-11-14 2010-02-23 Dharmacon, Inc. siRNA targeting connective tissue growth factor (CTGF)
US8883998B2 (en) * 2002-11-14 2014-11-11 Thermo Fisher Scientific Inc. siRNA targeting myeloid differentiation primary response gene (88) (MYD88)
US20100062951A1 (en) * 2002-11-14 2010-03-11 Dharmacon, Inc. siRNA targeting TIE-2
US7678896B2 (en) 2002-11-14 2010-03-16 Dharmacon, Inc. siRNA targeting serine/threonine kinase 12 (STK12 or aurora B kinase)
US20080113374A1 (en) * 2002-11-14 2008-05-15 Dharmacon, Inc. siRNA targeting fructose-1,6-bisphosphatase 1 (FBP1)
US20100075869A1 (en) * 2002-11-14 2010-03-25 Dharmacon, Inc. siRNA targeting TATA box binding protein (TBP)-associated factor (TAF1)
US20070207974A1 (en) * 2002-11-14 2007-09-06 Dharmacon Inc. Functional and hyperfunctional siRNA
US20080113377A1 (en) * 2002-11-14 2008-05-15 Dharmacon, Inc. siRNA Targeting proto-oncogene MET
US7691997B2 (en) 2002-11-14 2010-04-06 Dharmacon, Inc. Functional and hyperfunctional siRNA
US7691998B2 (en) 2002-11-14 2010-04-06 Dharmacon, Inc. siRNA targeting nucleoporin 62kDa (Nup62)
US20080108803A1 (en) * 2002-11-14 2008-05-08 Dharmacon Inc. Functional and hyperfunctional siRNA directed against Bcl-2
US20080108802A1 (en) * 2002-11-14 2008-05-08 Dharmacon Inc. Functional and hyperfunctional siRNA directed against Bcl-2
US7696344B2 (en) 2002-11-14 2010-04-13 Dharmacon, Inc. siRNA targeting complement factor B
US20100190971A1 (en) * 2002-11-14 2010-07-29 Dharmacon, Inc. siRNA Targeting Diacylglycerol O-Acyltransferase Homolog 2 (DGAT2)
US20070219362A1 (en) * 2002-11-14 2007-09-20 Dharmacon, Inc. siRNA targeting azurocidin 1 (Cartionic Antimicrobial protein 37)
US20080097090A1 (en) * 2002-11-14 2008-04-24 Dharmacon Inc. Functional and hyperfunctional siRNA directed against Bcl-2
US20070244311A1 (en) * 2002-11-14 2007-10-18 Dharmacon, Inc. siRNA targeting coatomer protein complex, subunit beta 2 (CPOB2)
US20100099578A1 (en) * 2002-11-14 2010-04-22 Dharmacon, Inc. siRNA Targeting Fructose-1, 6-bisphosphatase 1 (FBP1)
US20080091001A1 (en) * 2002-11-14 2008-04-17 Dharmacon Inc. Functional and hyperfunctional siRNA directed against Bcl-2
US20080091003A1 (en) * 2002-11-14 2008-04-17 Dharmacon Inc. Functional and hyperfunctional siRNA directed against Bcl-2
US20080090997A1 (en) * 2002-11-14 2008-04-17 Dharmacon, Inc. siRNA targeting complement component 3 (C3)
US7709629B2 (en) 2002-11-14 2010-05-04 Dharmacon, Inc. siRNA targeting diacylglycerol O-acyltransferase homolog 2 (DGAT2)
US20070265438A1 (en) * 2002-11-14 2007-11-15 Dharmacon, Inc. siRNA targeting polo-like kinase-1 (PLK-1)
US20070276135A1 (en) * 2002-11-14 2007-11-29 Dharmacon, Inc. siRNA targeting dual specificity phosphate 5 (DUSP5)
US20100113307A1 (en) * 2002-11-14 2010-05-06 Dharmacon, Inc. siRNA targeting vascular endothelial growth factor (VEGF)
US20100113306A1 (en) * 2002-11-14 2010-05-06 Dharmacon, Inc. siRNA Targeting connective tissue growth factor (CTGF)
US20100113760A1 (en) * 2002-11-14 2010-05-06 Dharmacon, Inc. siRNA targeting myeloid differentiation primary response gene (88) (MYD88)
US20080085998A1 (en) * 2002-11-14 2008-04-10 Dharmacon, Inc. siRNA targeting transient receptor potential cation channel, subfamily V, member 1 (TRPV1)
US8222396B2 (en) 2002-11-14 2012-07-17 Dharmacon, Inc. SiRNA targeting proto-oncogene MET
US7745611B2 (en) 2002-11-14 2010-06-29 Dharmacon, Inc. siRNA targeting KRAS
US20080071073A1 (en) * 2002-11-14 2008-03-20 Dharmacon, Inc. siRNA targeting ubiquitin ligases
US7745610B2 (en) 2002-11-14 2010-06-29 Dharmacon, Inc. siRNA targeting cyclin dependent kinase 11 (CDK11)
US7745612B2 (en) 2002-11-14 2010-06-29 Dharmacon, Inc. siRNA targeting interleukin-1 receptor-associated kinase 4 (IRAK4)
US7741470B2 (en) 2002-11-14 2010-06-22 Dharmacon, Inc. siRNA targeting gremlin
US20070276136A1 (en) * 2002-11-14 2007-11-29 Dharmacon, Inc. siRNA targeting serine/threonine kinase 12 (STK12 or aurora B kinase)
US20080064865A1 (en) * 2002-11-14 2008-03-13 Dharmacon, Inc. siRNA targeting cyclin dependent kinase 11 (CDK11)
US7737267B2 (en) 2002-11-14 2010-06-15 Dharmacon, Inc. siRNA targeting hypoxia-inducible factor 1
US20100144552A1 (en) * 2002-11-14 2010-06-10 Dharmacon, Inc. siRNA targeting serine/threonine kinase 12 (STK12 or aurora B kinase)
US20040191905A1 (en) * 2002-11-22 2004-09-30 University Of Massachusetts Modulation of HIV replication by RNA interference
US7618948B2 (en) * 2002-11-26 2009-11-17 Medtronic, Inc. Devices, systems and methods for improving and/or cognitive function through brain delivery of siRNA
US8415319B2 (en) 2002-11-26 2013-04-09 Medtronic, Inc. Devices, systems and methods for improving memory and/or cognitive function through brain delivery of siRNA
US20060178328A1 (en) * 2002-11-26 2006-08-10 Medtronic Inc. Devices, systems and methods for improving memory and/or cognitive function through brain delivery of siRNA
US8058251B2 (en) 2002-11-26 2011-11-15 Kaemmerer William F Devices, systems and methods for improving memory and/or cognitive function through brain delivery of siRNA
US8618069B2 (en) 2002-11-26 2013-12-31 Medtronic, Inc. Devices, systems and methods for improving memory and/or cognitive function through brain delivery of siRNA
US8119611B2 (en) 2002-11-26 2012-02-21 Medtronic, Inc. Treatment of neurodegenerative disease through intracranial delivery of SIRNA
US7829694B2 (en) 2002-11-26 2010-11-09 Medtronic, Inc. Treatment of neurodegenerative disease through intracranial delivery of siRNA
US7605249B2 (en) 2002-11-26 2009-10-20 Medtronic, Inc. Treatment of neurodegenerative disease through intracranial delivery of siRNA
US20070141009A1 (en) * 2003-01-03 2007-06-21 Shaharyar Khan Sirna mediated post-transriptional gene silencing of genes involved in alopecia
US20060178297A1 (en) * 2003-01-28 2006-08-10 Troy Carol M Systems and methods for silencing expression of a gene in a cell and uses thereof
US20040147027A1 (en) * 2003-01-28 2004-07-29 Troy Carol M. Complex for facilitating delivery of dsRNA into a cell and uses thereof
US8957198B2 (en) 2003-02-03 2015-02-17 Medtronic, Inc. Compositions, devices and methods for treatment of Huntington's disease through intracranial delivery of sirna
US7884084B2 (en) * 2003-02-10 2011-02-08 Aventis Pharma S.A. Oligonucleotides which inhibit expression of the OB-RGRP protein
US20050009042A1 (en) * 2003-02-10 2005-01-13 Aventis Pharma S.A. Oligonucleotides which inhibit expression of the OB-RGRP protein and method for detecting compounds which modify the interaction between proteins of the OB-RGRP family and the leptin receptor
US8796235B2 (en) 2003-02-21 2014-08-05 University Of South Florida Methods for attenuating dengue virus infection
US20060269530A1 (en) * 2003-02-21 2006-11-30 The Penn State Research Foundation RNA interference compositions and methods
US20060239971A1 (en) * 2003-02-21 2006-10-26 Mohapatra Shyam S Vectors for regulating gene expression
US20050164212A1 (en) * 2003-03-06 2005-07-28 Todd Hauser Modulation of gene expression using DNA-RNA hybrids
US20080085999A1 (en) * 2003-03-06 2008-04-10 Oligoengine, Inc. Modulation of gene expression using dna-rna hybrids
US20100093831A1 (en) * 2003-03-12 2010-04-15 Vasgene Therapeutics, Inc. Nucleic acid compounds for inhibiting angiogenesis and tumor growth
US20040248174A1 (en) * 2003-04-18 2004-12-09 Thetrustees Of The University Of Pennsylvania Compositions and methods for siRNA inhibition of angiopoietin 1and 2 and their receptor Tie2
US7994305B2 (en) * 2003-04-18 2011-08-09 The Trustees Of The University Of Pennsylvania Compositions and methods for siRNA inhibition of angiopoietin 1 and 2 and their receptor Tie2
US20070010468A1 (en) * 2003-04-23 2007-01-11 Georgetown University Methods and compositions for the inhibition of stat5 in prostate cancer cells
US20100278871A1 (en) * 2003-05-05 2010-11-04 Johns Hopkins University Anti-cancer dna vaccine employing plasmids encoding signal sequence, mutant oncoprotein antigen, and heat shock protein
US9701725B2 (en) 2003-05-05 2017-07-11 The Johns Hopkins University Anti-cancer DNA vaccine employing plasmids encoding signal sequence, mutant oncoprotein antigen, and heat shock protein
US8609830B2 (en) * 2003-05-16 2013-12-17 Merck Sharp & Dohme Corp. Methods and compositions for RNA interference
US20070149468A1 (en) * 2003-05-16 2007-06-28 Jackson Aimee L Methods and compositions for rna interference
US8299044B2 (en) 2003-05-19 2012-10-30 Genecare Research Institute Co., Ltd. Apoptosis inducer for cancer cell
US20100168209A1 (en) * 2003-05-19 2010-07-01 Genecare Research Institute Co., Ltd. Apoptosis inducer for cancer cell
US20070243570A1 (en) * 2003-05-19 2007-10-18 Genecare Research Institute Co., Ltd Apoptosis Inducer for Cancer Cell
US8809296B2 (en) 2003-05-19 2014-08-19 Genecare Research Institute Co., Ltd. Apoptosis inducer for cancer cell
US20090098614A1 (en) * 2003-06-02 2009-04-16 Zamore Phillip D Methods and Compositions for controlling Efficacy of RNA Silencing
US20100184828A1 (en) * 2003-06-02 2010-07-22 University Of Massachusetts Methods and compositions for enhancing the efficacy and specificity of rna silencing
US9121018B2 (en) 2003-06-02 2015-09-01 University Of Massachusetts Methods and compositions for enhancing the efficacy and specificity of RNA silencing
US7732593B2 (en) 2003-06-02 2010-06-08 University Of Massachusetts Methods and compositions for controlling efficacy of RNA silencing
US8309704B2 (en) 2003-06-02 2012-11-13 University Of Massachusetts Methods and compositions for enhancing the efficacy and specificity of RNAi
US8309705B2 (en) 2003-06-02 2012-11-13 University Of Massachusetts Methods and compositions for enhancing the efficacy and specificity of RNA silencing
US8329892B2 (en) 2003-06-02 2012-12-11 University Of Massachusetts Methods and compositions for enhancing the efficacy and specificity of RNA silencing
US7772203B2 (en) 2003-06-02 2010-08-10 University Of Massachusetts Methods and compositions for controlling efficacy of RNA silencing
US20050037988A1 (en) * 2003-06-02 2005-02-17 University Of Massachusetts Methods and compositions for controlling efficacy of RNA silencing
US10364429B2 (en) 2003-06-02 2019-07-30 University Of Massachusetts Methods and compositions for controlling efficacy of RNA silencing
US20100317105A1 (en) * 2003-06-02 2010-12-16 University Of Massachusetts Methods and compositions for controlling efficacy of RNA silencing
US20050181382A1 (en) * 2003-06-02 2005-08-18 University Of Massachusetts Methods and compositions for enhancing the efficacy and specificity of RNAi
US8304530B2 (en) 2003-06-02 2012-11-06 University Of Massachusetts Methods and compositions for enhancing the efficacy and specificity of RNA silencing
US10604754B2 (en) 2003-06-02 2020-03-31 University Of Massachusetts Methods and compositions for enhancing the efficacy and specificity of RNA silencing
US7459547B2 (en) 2003-06-02 2008-12-02 University Of Massachusetts Methods and compositions for controlling efficacy of RNA silencing
US20100184827A1 (en) * 2003-06-02 2010-07-22 University Of Massachusetts Methods and compositions for enhancing the efficacy and specificity of rna silencing
US20080318896A1 (en) * 2003-06-02 2008-12-25 University Of Massachusetts Methods and Compositions for Controlling of Efficacy of RNA Silencing
US20110152347A1 (en) * 2003-06-02 2011-06-23 University Of Massachusetts Methods and compositions for controlling efficacy of RNA silencing
US11459562B2 (en) 2003-06-02 2022-10-04 University Of Massachusetts Methods and compositions for controlling efficacy of RNA silencing
US7750144B2 (en) 2003-06-02 2010-07-06 University Of Massachusetts Methods and compositions for enhancing the efficacy and specificity of RNA silencing
US20100184826A1 (en) * 2003-06-02 2010-07-22 University Of Massachusetts Methods and compositions for enhancing the efficacy and specificity of rna silencing
US20090203896A1 (en) * 2003-06-03 2009-08-13 Balkrishen Bhat Modulation of survivin expression
US7541344B2 (en) * 2003-06-03 2009-06-02 Eli Lilly And Company Modulation of survivin expression
US20070161547A1 (en) * 2003-06-03 2007-07-12 Balkrishen Bhat Modulation of survivin expression
US20050019918A1 (en) * 2003-06-03 2005-01-27 Hidetoshi Sumimoto Treatment of cancer by inhibiting BRAF expression
US20110092565A1 (en) * 2003-06-09 2011-04-21 Alnylam Pharmaceuticals, Inc. Method of treating neurodegenerative disease
US10982212B2 (en) 2003-06-12 2021-04-20 Alnylam Pharmaceuticals, Inc. Conserved HBV and HCV sequences useful for gene silencing
US20100280102A1 (en) * 2003-06-13 2010-11-04 Alnylam Pharmaceuticals Double-stranded ribonucleic acid with increased effectiveness in an organism
WO2005044976A3 (en) * 2003-06-20 2006-02-23 Isis Pharmaceuticals Inc Oligomeric compounds for use in gene modulation
US20050256071A1 (en) * 2003-07-15 2005-11-17 California Institute Of Technology Inhibitor nucleic acids
US20050136430A1 (en) * 2003-07-15 2005-06-23 California Institute Of Technology Inhibitor nucleic acids
US20060240093A1 (en) * 2003-07-16 2006-10-26 Protiva Biotherapeutics, Inc. Lipid encapsulated interfering rna
US7982027B2 (en) * 2003-07-16 2011-07-19 Protiva Biotherapeutics, Inc. Lipid encapsulated interfering RNA
US7732590B2 (en) * 2003-08-18 2010-06-08 Isis Pharmaceuticals, Inc. Modulation of diacylglycerol acyltransferase 2 expression
US20050272680A1 (en) * 2003-08-18 2005-12-08 Sanjay Bhanot Modulation of diacylglycerol acyltransferase 2 expression
US20100311810A1 (en) * 2003-08-18 2010-12-09 Isis Pharmaceuticals, Inc. Modulation of diacylglycerol acyltransferase 2 expression
US8258289B2 (en) 2003-08-18 2012-09-04 Isis Pharmaceuticals, Inc Modulation of diacylglycerol acyltransferase 2 expression
US20050043524A1 (en) * 2003-08-18 2005-02-24 Isis Pharmaceuticals, Inc. Modulation of diacylglycerol acyltransferase 2 expression
US7825235B2 (en) 2003-08-18 2010-11-02 Isis Pharmaceuticals, Inc. Modulation of diacylglycerol acyltransferase 2 expression
US8883997B2 (en) 2003-08-18 2014-11-11 Isis Pharmaceuticals, Inc. Modulation of diacylglycerol acyltransferase 2 expression
US20070155658A1 (en) * 2003-08-25 2007-07-05 Nastech Pharmaceutical Company Inc. Nanoparticles for delivery of nucleic acids and stable double-stranded rna
US20060160123A1 (en) * 2003-08-25 2006-07-20 Nastech Pharmaceutical Company Inc. Method of minimizing off-target effects of siRNA molecules
US20060122137A1 (en) * 2003-08-25 2006-06-08 Nastech Pharmaceutical Company Inc. 5'-methylpyrimidine and 2'-O-methyl ribonucleotide modified double-stranded ribonucleic acid molecules
US20060142230A1 (en) * 2003-08-25 2006-06-29 Nastech Pharmaceutical Company Inc. Double-stranded ribonucleic acid molecules having ribothymidine
US20050136437A1 (en) * 2003-08-25 2005-06-23 Nastech Pharmaceutical Company Inc. Nanoparticles for delivery of nucleic acids and stable double-stranded RNA
US20090192113A1 (en) * 2003-08-28 2009-07-30 Jan Weiler Interfering RNA Duplex Having Blunt-Ends and 3`-Modifications
US8097716B2 (en) 2003-08-28 2012-01-17 Novartis Ag Interfering RNA duplex having blunt-ends and 3′-modifications
US8680063B2 (en) 2003-09-12 2014-03-25 University Of Massachusetts RNA interference for the treatment of gain-of-function disorders
US9434943B2 (en) 2003-09-12 2016-09-06 University Of Massachusetts RNA interference for the treatment of gain-of-function disorders
US11299734B2 (en) 2003-09-12 2022-04-12 University Of Massachusetts RNA interference for the treatment of gain-of-function disorders
US7947658B2 (en) 2003-09-12 2011-05-24 University Of Massachusetts RNA interference for the treatment of gain-of-function disorders
US20110172291A1 (en) * 2003-09-12 2011-07-14 University Of Massachusetts Rna interference for the treatment of gain-of-function disorders
US10344277B2 (en) 2003-09-12 2019-07-09 University Of Massachusetts RNA interference for the treatment of gain-of-function disorders
US20090118206A1 (en) * 2003-09-12 2009-05-07 University Of Massachusetts Rna interference for the treatment of gain-of-function disorders
US20080249038A1 (en) * 2003-10-07 2008-10-09 Quark Biotech, Inc. Bone Morphogenetic Protein (Bmp) 2A and Uses Thereof
US20070258993A1 (en) * 2003-11-12 2007-11-08 The Austin Research Institute Dna-Carrier Conjugate
US20100260730A1 (en) * 2003-11-20 2010-10-14 University Of South Florida SHIP-Deficiency to Increase Megakaryocyte Progenitor Production
US7763592B1 (en) 2003-11-20 2010-07-27 University Of South Florida SHIP-deficiency to increase megakaryocyte progenitor production
US20110064704A1 (en) * 2003-11-20 2011-03-17 University Of South Florida Ship-deficiency to increase megakaryocyte and platelet production
US7807646B1 (en) 2003-11-20 2010-10-05 University Of South Florida SHIP-deficiency to increase megakaryocyte progenitor production
US8008273B2 (en) 2003-11-20 2011-08-30 University Of South Florida SHIP-deficiency to increase megakaryocyte progenitor production
US20050208658A1 (en) * 2003-11-21 2005-09-22 The University Of Maryland RNA interference mediated inhibition of 11beta hydroxysteriod dehydrogenase-1 (11beta HSD-1) gene expression
US7732591B2 (en) 2003-11-25 2010-06-08 Medtronic, Inc. Compositions, devices and methods for treatment of huntington's disease through intracranial delivery of sirna
US11359196B2 (en) 2003-11-26 2022-06-14 University Of Massachusetts Sequence-specific inhibition of small RNA function
US8685946B2 (en) 2003-11-26 2014-04-01 Universiy of Massachusetts Sequence-specific inhibition of small RNA function
US20050227256A1 (en) * 2003-11-26 2005-10-13 Gyorgy Hutvagner Sequence-specific inhibition of small RNA function
US9334497B2 (en) 2003-11-26 2016-05-10 University Of Massachusetts Sequence-specific inhibition of small RNA function
US20080171051A1 (en) * 2003-11-26 2008-07-17 Patrick Gerard Johnston Cancer Treatment
US20090083873A1 (en) * 2003-11-26 2009-03-26 Uinversity Of Massachusetts Sequence-specific inhibition of small rna function
US8598143B2 (en) * 2003-11-26 2013-12-03 University Of Massachusetts Sequence-specific inhibition of small RNA function
US9089590B2 (en) 2003-12-04 2015-07-28 University Of South Florida Polynucleotides for reducing respiratory syncytial virus gene expression
US20070238676A1 (en) * 2003-12-04 2007-10-11 Mohapatra Shyam S Polynucleotides for Reducing Respiratory Syncytial Virus Gene Expression
US20050234000A1 (en) * 2003-12-12 2005-10-20 Mitchell Gordon S SiRNA delivery into mammalian nerve cells
US9879253B2 (en) 2003-12-22 2018-01-30 University Of Massachusetts Methods and compositions for enhancing the efficacy and specificity of single and double blunt-ended siRNA
US10385339B2 (en) 2003-12-22 2019-08-20 University Of Massachusetts Methods and compositions for enhancing the efficacy and specificity of single and double blunt-ended siRNA
US7807814B2 (en) * 2003-12-23 2010-10-05 The Trustees Of The University Of Pennsylvania Compositions and methods for combined therapy of disease
US20060287259A1 (en) * 2003-12-23 2006-12-21 The Trustees Of The University Of Pennsylvania Compositions and methods for combined therapy of disease
US20090082303A1 (en) * 2004-01-16 2009-03-26 Takeda Pharmaceutical Company Limited Drug for preventing and treating atherosclerosis
US20070161586A1 (en) * 2004-01-16 2007-07-12 Takeda Pharmaceutical Company Limited Drug for preventing and treating atherosclerosis
US7939652B2 (en) * 2004-01-30 2011-05-10 Quark Pharmaceuticals Inc. Oligoribonucleotides and methods of use thereof for treatment of fibrotic conditions and other diseases
US20080287386A1 (en) * 2004-01-30 2008-11-20 Quark Biotech, Inc. Oligoribonucleotides and methods of use thereof for treatment of fibrotic conditions and other diseases
US8198258B2 (en) 2004-01-30 2012-06-12 Quark Pharmaceuticals Inc. Oligoribonucleotides and methods of use thereof for treatment of fibrotic conditions and other diseases
US7858769B2 (en) * 2004-02-10 2010-12-28 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using multifunctional short interfering nucleic acid (multifunctional siNA)
US20110118335A1 (en) * 2004-02-10 2011-05-19 Vasant Jadhav RNA Interference Mediated Inhibition Of Gene Expression Using Multifunctional Short Interfering Nucleic Acid (Multifunctional siNA)
US20080039412A1 (en) * 2004-02-10 2008-02-14 Sirna Therapeutics, Inc. Rna Interference Mediated Inhibition of Gene Expression Using Multifunctional Short Interfering Nucleic Acid (Multifunctional Sina)
US20060069050A1 (en) * 2004-02-17 2006-03-30 University Of Massachusetts Methods and compositions for mediating gene silencing
US20050273868A1 (en) * 2004-02-17 2005-12-08 University Of Massachusetts Methods and compositions for enhancing RISC activity in vitro and in vivo
US20080039411A1 (en) * 2004-02-23 2008-02-14 Ulf Smith Use Of Resistin Antisense Oligonucleotides And/Or Sirna Molecules In The Treatment Of Rheumatoid Arthritis
US20070202134A1 (en) * 2004-02-23 2007-08-30 Kufe Donald W Muc1 Antagonist Enhancement of Death Receptor Ligand-Induced Apoptosis
US8569474B2 (en) 2004-03-09 2013-10-29 Isis Pharmaceuticals, Inc. Double stranded constructs comprising one or more short strands hybridized to a longer strand
US8293719B2 (en) 2004-03-12 2012-10-23 Alnylam Pharmaceuticals, Inc. iRNA agents targeting VEGF
US20050202075A1 (en) * 2004-03-12 2005-09-15 Pardridge William M. Delivery of genes encoding short hairpin RNA using receptor-specific nanocontainers
US9365849B2 (en) 2004-03-15 2016-06-14 Integrated Dna Technologies, Inc. Methods and compositions for the specific inhibition of gene expression by double-stranded RNA
US20070265220A1 (en) * 2004-03-15 2007-11-15 City Of Hope Methods and compositions for the specific inhibition of gene expression by double-stranded RNA
US20050277610A1 (en) * 2004-03-15 2005-12-15 City Of Hope Methods and compositions for the specific inhibition of gene expression by double-stranded RNA
US9518262B2 (en) 2004-03-15 2016-12-13 City Of Hope Methods and compositions for the specific inhibition of gene expression by double-stranded RNA
US8796444B2 (en) 2004-03-15 2014-08-05 City Of Hope Methods and compositions for the specific inhibition of gene expression by double-stranded RNA
US9988630B2 (en) 2004-03-15 2018-06-05 City Of Hope Methods and compositions for the specific inhibition of gene expression by double-stranded RNA
US8658356B2 (en) 2004-03-15 2014-02-25 City Of Hope Methods and compositions for the specific inhibition of gene expression by double-stranded RNA
US10106792B2 (en) 2004-03-15 2018-10-23 City Of Hope Methods and compositions for the specific inhibition of gene expression by double-stranded RNA
US8809515B2 (en) 2004-03-15 2014-08-19 City Of Hope Methods and compositions for the specific inhibition of gene expression by double-stranded RNA
US20090326046A1 (en) * 2004-03-15 2009-12-31 City Of Hope Methods and compositions for the specific inhibition of gene expression by double-stranded rna
US20100004318A1 (en) * 2004-03-15 2010-01-07 Integrated Dna Technologies, Inc. Methods and compositions for the specific inhibition of gene expression by double-stranded rna
EP2514758A1 (en) * 2004-03-15 2012-10-24 City of Hope Methods and compositions for the specific inhibition of gene expression by double-stranded RNA
US20100004436A1 (en) * 2004-03-15 2010-01-07 Integrated Dna Technologies, Inc. Methods and compositions for the specific inhibition of gene expression by double-stranded rna
US8084599B2 (en) 2004-03-15 2011-12-27 City Of Hope Methods and compositions for the specific inhibition of gene expression by double-stranded RNA
US8691786B2 (en) 2004-03-15 2014-04-08 City Of Hope Methods and compositions for the specific inhibition of gene expression by double-stranded RNA
US20090325285A1 (en) * 2004-03-15 2009-12-31 City Of Hope Methods and compositions for the specific inhibition of gene expression by double-stranded rna
US20110213328A1 (en) * 2004-03-18 2011-09-01 Medtronic, Inc. Methods and Systems for Treatment of Neurological Diseases of the Central Nervous System
US20050272682A1 (en) * 2004-03-22 2005-12-08 Evers Bernard M SiRNA targeting PI3K signal transduction pathway and siRNA-based therapy
US7851452B2 (en) * 2004-03-22 2010-12-14 The Trustees Of The University Of Pennsylvania Methods of use of bcl-6-derived nucleotides to induce apoptosis
US20080153765A1 (en) * 2004-03-22 2008-06-26 Gewirtz Alan M Methods of Use of Bcl-6-Derived Nucleotides to Induce Apoptosis
US7872117B2 (en) * 2004-03-26 2011-01-18 Van Andel Research Institute c-met siRNA adenovirus vectors inhibit cancer cell growth, invasion and tumorigenicity
US20070232555A1 (en) * 2004-03-26 2007-10-04 Nariyoshi Shinomiya C-Met Sirna Adenovirus Vectors Inhibit Cancer Cell Growth, Invasion and Tumorigenicity
US20050256076A1 (en) * 2004-03-26 2005-11-17 Curis, Inc. RNA interference modulators of hedgehog signaling and uses thereof
US8802639B2 (en) * 2004-03-26 2014-08-12 Curis, Inc. RNA interference modulators of hedgehog signaling and uses thereof
US20080167256A1 (en) * 2004-03-31 2008-07-10 Hidetoshi Sumimoto Cancer Therapy Via the Inhibition of Skp-2 Expression
US8431693B2 (en) 2004-04-05 2013-04-30 Alnylam Pharmaceuticals, Inc. Process for desilylation of oligonucleotides
US8063198B2 (en) 2004-04-05 2011-11-22 Alnylam Pharmaceuticals, Inc. Processes and reagents for desilylation of oligonucleotides
US8058448B2 (en) 2004-04-05 2011-11-15 Alnylam Pharmaceuticals, Inc. Processes and reagents for sulfurization of oligonucleotides
US8703930B2 (en) 2004-04-09 2014-04-22 Genecare Research Institute Co., Ltd. Cancer cell-specific apoptosis-inducing agents that target chromosome stabilization-associated genes
US20060078902A1 (en) * 2004-04-15 2006-04-13 Michaeline Bunting Method and compositions for RNA interference
US20060014289A1 (en) * 2004-04-20 2006-01-19 Nastech Pharmaceutical Company Inc. Methods and compositions for enhancing delivery of double-stranded RNA or a double-stranded hybrid nucleic acid to regulate gene expression in mammalian cells
US7626014B2 (en) 2004-04-27 2009-12-01 Alnylam Pharmaceuticals Single-stranded and double-stranded oligonucleotides comprising a 2-arylpropyl moiety
US20100197899A1 (en) * 2004-04-27 2010-08-05 Alnylam Pharmaceuticals, Inc. Single-stranded and double-stranded oligonucleotides comprising a 2-arylpropyl moiety
US8470988B2 (en) 2004-04-27 2013-06-25 Alnylam Pharmaceuticals, Inc. Single-stranded and double-stranded oligonucleotides comprising a 2-arylpropyl moiety
US7674778B2 (en) 2004-04-30 2010-03-09 Alnylam Pharmaceuticals Oligonucleotides comprising a conjugate group linked through a C5-modified pyrimidine
US20060040882A1 (en) * 2004-05-04 2006-02-23 Lishan Chen Compostions and methods for enhancing delivery of nucleic acids into cells and for modifying expression of target genes in cells
US20050260214A1 (en) * 2004-05-12 2005-11-24 Simon Michael R Composition and method for introduction of RNA interference sequences into targeted cells and tissues
US20070141601A1 (en) * 2004-05-12 2007-06-21 Dharmacon, Inc. siRNA targeting cAMP-specific phosphodiesterase 4D
US20110117088A1 (en) * 2004-05-12 2011-05-19 Simon Michael R Composition and method for introduction of rna interference sequences into targeted cells and tissues
US20070207491A1 (en) * 2004-05-12 2007-09-06 Dharmacon, Inc. siRNA targeting minichromosome maintenance deficient 4 (MCM4)
US7605250B2 (en) 2004-05-12 2009-10-20 Dharmacon, Inc. siRNA targeting cAMP-specific phosphodiesterase 4D
US20060030003A1 (en) * 2004-05-12 2006-02-09 Simon Michael R Composition and method for introduction of RNA interference sequences into targeted cells and tissues
US7709616B2 (en) 2004-05-14 2010-05-04 Rosetta Genomics Inc. Micrornas and uses thereof
US8455633B2 (en) 2004-05-26 2013-06-04 Rosetta Genomics Ltd. Viral and viral associated mirnas and uses thereof
US20070003575A1 (en) * 2004-05-26 2007-01-04 Itzhak Bentwich Viral and viral associated MiRNAs and uses thereof
US7795419B2 (en) 2004-05-26 2010-09-14 Rosetta Genomics Ltd. Viral and viral associated miRNAs and uses thereof
US8465914B2 (en) 2004-05-28 2013-06-18 Asuragen, Inc. Method and compositions involving microRNA
US7888010B2 (en) 2004-05-28 2011-02-15 Asuragen, Inc. Methods and compositions involving microRNA
US7919245B2 (en) 2004-05-28 2011-04-05 Asuragen, Inc. Methods and compositions involving microRNA
US8003320B2 (en) 2004-05-28 2011-08-23 Asuragen, Inc. Methods and compositions involving MicroRNA
US8568971B2 (en) 2004-05-28 2013-10-29 Asuragen, Inc. Methods and compositions involving microRNA
US10047388B2 (en) 2004-05-28 2018-08-14 Asuragen, Inc. Methods and compositions involving MicroRNA
US8394947B2 (en) 2004-06-03 2013-03-12 Isis Pharmaceuticals, Inc. Positionally modified siRNA constructs
US20060083780A1 (en) * 2004-06-07 2006-04-20 Protiva Biotherapeutics, Inc. Cationic lipids and methods of use
US20060008910A1 (en) * 2004-06-07 2006-01-12 Protiva Biotherapeuties, Inc. Lipid encapsulated interfering RNA
US9926560B2 (en) 2004-06-07 2018-03-27 Protiva Biotherapeutics, Inc. Lipid encapsulating interfering RNA
US20110060032A1 (en) * 2004-06-07 2011-03-10 Protiva Biotherapeutics, Inc. Lipid encapsulating interfering rna
US9181545B2 (en) 2004-06-07 2015-11-10 Protiva Biotherapeutics, Inc. Lipid encapsulating interfering RNA
US7745651B2 (en) 2004-06-07 2010-06-29 Protiva Biotherapeutics, Inc. Cationic lipids and methods of use
US7799565B2 (en) 2004-06-07 2010-09-21 Protiva Biotherapeutics, Inc. Lipid encapsulated interfering RNA
US20060008907A1 (en) * 2004-06-09 2006-01-12 The Curators Of The University Of Missouri Control of gene expression via light activated RNA interference
US20060051815A1 (en) * 2004-06-25 2006-03-09 The J. David Gladstone Institutes Methods of treating smooth muscle cell disorders
US8114853B2 (en) 2004-06-25 2012-02-14 The J. David Gladstone Institutes Methods of treating smooth muscle cell disorders
US20100022624A1 (en) * 2004-06-25 2010-01-28 Verdin Eric M Methods of Treating Smooth Muscle Cell Disorders
US7615618B2 (en) 2004-06-30 2009-11-10 Alnylam Pharmaceuticals, Inc. Oligonucleotides comprising a non-phosphate backbone linkage
US7723512B2 (en) 2004-06-30 2010-05-25 Alnylam Pharmaceuticals Oligonucleotides comprising a non-phosphate backbone linkage
US8013136B2 (en) 2004-06-30 2011-09-06 Alnylam Pharmaceuticals, Inc. Oligonucleotides comprising a non-phosphate backbone linkage
US7807815B2 (en) 2004-07-02 2010-10-05 Protiva Biotherapeutics, Inc. Compositions comprising immunostimulatory siRNA molecules and DLinDMA or DLenDMA
US20060025366A1 (en) * 2004-07-02 2006-02-02 Protiva Biotherapeutics, Inc. Immunostimulatory siRNA molecules and uses therefor
US8143228B2 (en) * 2004-07-12 2012-03-27 Medical Research Fund Of Tel Aviv Sourasky Medical Center Agents capable of downregulating an MSF-A dependent HIF-1α and use thereof in cancer treatment
US8785373B2 (en) 2004-07-12 2014-07-22 The Medical Research, Infrastructure and Health Services Fund of the Tel Aviv Medical Center Agents capable of downregulating an MSF-A-dependent HIF-1alpha and use thereof in cancer treatment
US20090098127A1 (en) * 2004-07-12 2009-04-16 Medical Research Fund Of Tel Aviv Sourasky Medical Center Agents Capable of Downregulating an MSF-A DEPENDENT HIF-1ALPHA and Use Thereof in Cancer Treatment
US20060269519A1 (en) * 2004-07-19 2006-11-30 Baylor College Of Medicine Modulation of cytokine signaling regulators and applications for immunotherapy
US7868158B2 (en) 2004-07-19 2011-01-11 Baylor College Of Medicine Modulation of cytokine signaling regulators and applications for immunotherapy
US7579451B2 (en) 2004-07-21 2009-08-25 Alnylam Pharmaceuticals, Inc. Oligonucleotides comprising a modified or non-natural nucleobase
US7772387B2 (en) 2004-07-21 2010-08-10 Alnylam Pharmaceuticals Oligonucleotides comprising a modified or non-natural nucleobase
US7632932B2 (en) 2004-08-04 2009-12-15 Alnylam Pharmaceuticals, Inc. Oligonucleotides comprising a ligand tethered to a modified or non-natural nucleobase
US7893224B2 (en) 2004-08-04 2011-02-22 Alnylam Pharmaceuticals, Inc. Oligonucleotides comprising a ligand tethered to a modified or non-natural nucleobase
WO2006020557A3 (en) * 2004-08-10 2007-08-16 Immusol Inc Methods of using or identifying agents that inhibit cancer growth
WO2006020557A2 (en) * 2004-08-10 2006-02-23 Immusol, Inc. Methods of using or identifying agents that inhibit cancer growth
US20060063181A1 (en) * 2004-08-13 2006-03-23 Green Pamela J Method for identification and quantification of short or small RNA molecules
US20100022623A1 (en) * 2004-08-23 2010-01-28 Sylentis S.A.U. Methods and Compositions for the Treatment of Eye Disorders with Increased Intraocular Pressure
US20090099112A1 (en) * 2004-08-23 2009-04-16 Sylentis S.A.U. Methods and Compositions for the Treatment of Eye Disorders with Increased Intraocular Pressure
US8389490B2 (en) 2004-08-23 2013-03-05 Sylentis S.A.U. Methods and compositions for the treatment of eye disorders with increased intraocular pressure
US20090105175A1 (en) * 2004-08-23 2009-04-23 Sylentis S.A.U. Methods and Compositions for the Treatment of Eye Disorders with Increased Intraocular Pressure
US8030284B2 (en) 2004-08-23 2011-10-04 Sylentis S.A.U. Treatment of eye disorders characterized by an elevated intraocular pressure by siRNAs
US20090105176A1 (en) * 2004-08-23 2009-04-23 Sylentis S.A.U. Methods and Compositions for the Treatment of Eye Disorders with Increased Intraocular Pressure
US20100331394A1 (en) * 2004-08-23 2010-12-30 Sylentis S.A.U. Methods and Compositions for the Treatment of Eye Disorders with Increased Intraocular Pressure
US7902169B2 (en) 2004-08-23 2011-03-08 Sylentis S.A.U. Methods and compositions for the treatment of eye disorders with increased intraocular pressure
US20090099111A1 (en) * 2004-08-23 2009-04-16 Sylentis S.A.U. Methods and Compositions for the Treatment of Eye Disorders with Increased Intraocular Pressure
US8198250B2 (en) * 2004-08-23 2012-06-12 Sylentis S.A.U. Methods and compositions for the treatment of eye disorders with increased intraocular pressure
US8258110B2 (en) * 2004-08-23 2012-09-04 Sylentis S.A.U. Methods and compositions for the treatment of eye disorders with increased intraocular pressure
US20090099113A1 (en) * 2004-08-23 2009-04-16 Sylentis S.A.U Methods and Compositions for the Treatment of Eye Disorders with Increased Intraocular Pressure
US8252758B2 (en) * 2004-08-23 2012-08-28 Sylentis S.A.U. Methods and compositions for the treatment of eye disorders with increased intraocular pressure
US8252759B2 (en) * 2004-08-23 2012-08-28 Sylentis S.A.U. Methods and compositions for the treatment of eye disorders with increased intraocular pressure
US20090099114A1 (en) * 2004-08-23 2009-04-16 Sylentis S.A.U. Methods and Compositions for the Treatment of Eye Disorders with Increased Intraocular Pressure
US8247387B2 (en) * 2004-08-23 2012-08-21 Sylentis S.A.U. Methods and compositions for the treatment of eye disorders with increased intraocular pressure
US8247386B2 (en) * 2004-08-23 2012-08-21 Sylentis Sau Methods and compositions for the treatment of eye disorders with increased intraocular pressure
US8951982B2 (en) 2004-08-23 2015-02-10 Sylentis S.A.U. Methods and compositions for the treatment of eye disorders with increased intraocular pressure
US20090005330A1 (en) * 2004-08-31 2009-01-01 Sylentis S.A. Methods and Compositions to Inhibit P2x7 Receptor Expression
US20090264501A9 (en) * 2004-08-31 2009-10-22 Sylentis S.A. Methods and Compositions to Inhibit P2x7 Receptor Expression
US7884086B2 (en) * 2004-09-08 2011-02-08 Isis Pharmaceuticals, Inc. Conjugates for use in hepatocyte free uptake assays
US20090170794A1 (en) * 2004-09-10 2009-07-02 Somagenics Inc. Small interfering rnas that efficiently inhibit viral expression and methods of use thereof
US20070149470A1 (en) * 2004-09-10 2007-06-28 Kaspar Roger L Inhibition of viral gene expression using small interfering RNA
US8426380B2 (en) 2004-09-10 2013-04-23 Somagenics, Inc. Inhibition of viral gene expression using small interfering RNA
US7902351B2 (en) * 2004-09-10 2011-03-08 Somagenics Inc. Inhibition of viral gene expression using small interfering RNA
US8569374B2 (en) 2004-09-16 2013-10-29 The Trustees Of The University Of Pennsylvania NADPH oxidase inhibition pharmacotherapies for obstructive sleep apnea syndrome and its associated morbidities
WO2006033965A2 (en) * 2004-09-16 2006-03-30 The Trustees Of The University Of Pennsylvania Nadph oxidase inhibition pharmacotherapies for obstructive sleep apnea syndrome and its associated morbidities
WO2006033965A3 (en) * 2004-09-16 2006-12-21 Univ Pennsylvania Nadph oxidase inhibition pharmacotherapies for obstructive sleep apnea syndrome and its associated morbidities
US20060154856A1 (en) * 2004-09-16 2006-07-13 Veasey Sigrid C NADPH oxidase inhibition pharmacotherapies for Obstructive Sleep Apnea syndrome and its associated morbidities
US8188061B2 (en) 2004-09-24 2012-05-29 Alnylam Pharmaceuticals, Inc. RNAi modulation of APOB and uses thereof
US8592571B2 (en) 2004-09-24 2013-11-26 Alnylam Pharmaceuticals, Inc. RNAi modulation of APOB and uses thereof
US20100216866A1 (en) * 2004-09-24 2010-08-26 Alnylam Pharmaceuticals, Inc. RNAi Modulation of APOB and Uses Thereof
US9187747B2 (en) 2004-09-24 2015-11-17 Alnylam Pharmaceuticals, Inc. RNAi modulation of ApoB and uses thereof
US20100113332A1 (en) * 2004-09-27 2010-05-06 Nastech Pharmaceutical Company Inc. Method of treating an inflammatory disease by double stranded ribonucleic acid
US20090028862A1 (en) * 2004-09-30 2009-01-29 Arndt Gregory M Emmprin antagonists and uses thereof
US20060089323A1 (en) * 2004-10-22 2006-04-27 Sailen Barik RNAi modulation of RSV, PIV and other respiratory viruses and uses thereof
US20110021606A1 (en) * 2004-10-22 2011-01-27 South Alabama Medical Science Foundation RNAi Modulation of RSV, PIV and Other Respiratory Viruses and Uses Thereof
US7943755B2 (en) * 2004-10-22 2011-05-17 Neuregenix Limited Neuron regeneration
US8598134B2 (en) * 2004-10-22 2013-12-03 South Alabama Medical Science Foundation RNAi modulation of RSV, PIV and other respiratory viruses and uses thereof
US20080253989A1 (en) * 2004-10-22 2008-10-16 Neuregenix Limited Neuron Regeneration
US20060089324A1 (en) * 2004-10-22 2006-04-27 Sailen Barik RNAi modulation of RSV, PIV and other respiratory viruses and uses thereof
US7790878B2 (en) 2004-10-22 2010-09-07 Alnylam Pharmaceuticals, Inc. RNAi modulation of RSV, PIV and other respiratory viruses and uses thereof
US20060189564A1 (en) * 2004-10-22 2006-08-24 Medtronic, Inc. Methods and sequences to suppress pro-inflamatory cytokine actions locally to treat pain
US20100144833A1 (en) * 2004-10-22 2010-06-10 Sailen Barik RNAi Modulation Of RSV, PIV And Other Respiratory Viruses And Uses Thereof
US7592322B2 (en) 2004-10-22 2009-09-22 Alnylam Pharmaceuticals, Inc. RNAi modulation of RSV, PIV and other respiratory viruses and uses thereof
US20060094676A1 (en) * 2004-10-29 2006-05-04 Ronit Lahav Compositions and methods for treating cancer using compositions comprising an inhibitor of endothelin receptor activity
US9506061B2 (en) 2004-11-12 2016-11-29 Asuragen, Inc. Methods and compositions involving miRNA and miRNA inhibitor molecules
US8173611B2 (en) 2004-11-12 2012-05-08 Asuragen Inc. Methods and compositions involving miRNA and miRNA inhibitor molecules
US9447414B2 (en) 2004-11-12 2016-09-20 Asuragen, Inc. Methods and compositions involving miRNA and miRNA inhibitor molecules
US8058250B2 (en) 2004-11-12 2011-11-15 Asuragen, Inc. Methods and compositions involving miRNA and miRNA inhibitor molecules
US7960359B2 (en) 2004-11-12 2011-06-14 Asuragen, Inc. Methods and compositions involving miRNA and miRNA inhibitor molecules
US8765709B2 (en) 2004-11-12 2014-07-01 Asuragen, Inc. Methods and compositions involving miRNA and miRNA inhibitor molecules
US8946177B2 (en) 2004-11-12 2015-02-03 Mima Therapeutics, Inc Methods and compositions involving miRNA and miRNA inhibitor molecules
US20080171715A1 (en) * 2004-11-12 2008-07-17 David Brown Methods and compositions involving mirna and mirna inhibitor molecules
US8809287B2 (en) * 2004-11-15 2014-08-19 Icahn School Of Medicine At Mount Sinai Compositions and methods for altering Wnt autocrine signaling
US20090163407A1 (en) * 2004-11-15 2009-06-25 Mount Sinai School Of Medicine Of New York University Compositions and methods for altering wnt autocrine signaling
US20060134189A1 (en) * 2004-11-17 2006-06-22 Protiva Biotherapeutics, Inc siRNA silencing of apolipoprotein B
US20130131331A1 (en) * 2004-11-19 2013-05-23 Genecare Research Institute Co., Ltd. Cancer-cell-specific cytostatic agent
US7935811B2 (en) 2004-11-22 2011-05-03 Dharmacon, Inc. Apparatus and system having dry gene silencing compositions
US7923206B2 (en) * 2004-11-22 2011-04-12 Dharmacon, Inc. Method of determining a cellular response to a biological agent
US7994307B2 (en) 2004-11-24 2011-08-09 Alnylam Pharmaceuticals, Inc. RNAi modulation of the BCR-ABL fusion gene and uses thereof
US20100234446A1 (en) * 2004-11-24 2010-09-16 Philipp Hadwiger RNAi Modulation of the BCR-ABL Fusion Gene and Uses Thereof
US20100256218A1 (en) * 2004-12-14 2010-10-07 Olaf Heidenreich RNAi MODULATION OF MLL-AF4 AND USES THEREOF
US8034793B2 (en) 2004-12-14 2011-10-11 Alnylam Pharmaceuticals, Inc. RNAi modulation of MLL-AF4 and uses thereof
US9181546B2 (en) 2004-12-17 2015-11-10 Beth Israel Deaconess Medical Center Compositions for bacterial mediated gene silencing and methods of using same
US20060134787A1 (en) * 2004-12-22 2006-06-22 University Of Massachusetts Methods and compositions for enhancing the efficacy and specificity of single and double blunt-ended siRNA
US8058255B2 (en) 2004-12-23 2011-11-15 Applied Biosystems, Llc Methods and compositions concerning siRNA's as mediators of RNA interference
US9011866B2 (en) * 2005-01-06 2015-04-21 The Johns Hopkins University RNA interference that blocks expression of pro-apoptotic proteins potentiates immunity induced by DNA and transfected dendritic cell vaccines
US20080069840A1 (en) * 2005-01-06 2008-03-20 Tzyy-Choou Wu RNA Interference That Blocks Expression of Pro-Apoptotic Proteins Potentiates Immunity Induced by DNA and Transfected Dendritic Cell Vaccines
WO2006074346A3 (en) * 2005-01-07 2007-07-05 Alnylam Pharmaceuticals Inc RNAi MODULATION OF RSV AND THERAPEUTIC USES THEREOF
US20090240043A1 (en) * 2005-01-07 2009-09-24 Alnylam Pharmaceuticals, Inc. RNAi MODULATION OF RSV AND THERAPEUTIC USES THEREOF
US20060258608A1 (en) * 2005-01-07 2006-11-16 Rachel Meyers RNAi modulation of RSV and therapeutic uses thereof
US20060166921A1 (en) * 2005-01-07 2006-07-27 Rachel Meyers RNAi modulation of RSV and therapeutic uses thereof
US7981869B2 (en) 2005-01-07 2011-07-19 Alnylam Pharmaceuticals, Inc. RNAi modulation of RSV and therapeutic uses thereof
US8158773B2 (en) 2005-01-07 2012-04-17 Alnylam Pharmaceuticals, Inc. RNAi modulation of RSV and therapeutic uses thereof
US8859750B2 (en) 2005-01-07 2014-10-14 Alnylam Pharmaceuticals, Inc. RNAi modulation of RSV and therapeutic uses thereof
US7507809B2 (en) * 2005-01-07 2009-03-24 Alnylam Pharmaceuticals, Inc. RNAi modulation of RSV and therapeutic uses thereof
US8263572B2 (en) 2005-01-07 2012-09-11 Alnylam Pharmaceuticals, Inc. RNAi modulation of RSV and therapeutic uses thereof
US7517865B2 (en) * 2005-01-07 2009-04-14 Alnylam Pharmaceuticals, Inc. RNAi modulation of RSV and therapeutic uses thereof
US20090233984A1 (en) * 2005-01-07 2009-09-17 Alnylam Pharmaceuticals, Inc. RNAi Modulation of RSV and Therapeutic Uses Thereof
US20060217324A1 (en) * 2005-01-24 2006-09-28 Juergen Soutschek RNAi modulation of the Nogo-L or Nogo-R gene and uses thereof
US20080102084A1 (en) * 2005-01-26 2008-05-01 Tzyy-Choou Wu Anti-cancer DNA Vaccine Employing Plasmids Encoding Mutant Oncoprotein Antigen and Calreticulin
US20060172965A1 (en) * 2005-02-01 2006-08-03 Alcon, Inc. RNAi-mediated inhibition of ocular targets
US20060172963A1 (en) * 2005-02-01 2006-08-03 Alcon, Inc. RNAi-mediated inhibition of ocular hypertension targets
US20110124708A1 (en) * 2005-02-01 2011-05-26 Alcon Inc. Rnai-mediated inhibition of ocular hypertension targets
US7592324B2 (en) * 2005-02-01 2009-09-22 Alcon, Inc. RNAi-mediated inhibition of ocular targets
US20080070857A1 (en) * 2005-02-14 2008-03-20 Jun Nishihira Pharmaceutical Agents for Preventing Metastasis of Cancer
US20110190381A1 (en) * 2005-03-11 2011-08-04 Alcon Inc. Rnai-mediated inhibition of frizzled related protein-1 for treatment of glaucoma
US9550994B2 (en) 2005-03-11 2017-01-24 Arrowhead Pharmaceuticals, Inc. RNAI-mediated inhibition of frizzled related protein-1 for treatment of glaucoma
US20110003879A1 (en) * 2005-03-11 2011-01-06 Vincent Mark D Antisense oligonucleotides targeted to the coding region of thymidylate synthase and uses thereof
US20060223773A1 (en) * 2005-03-11 2006-10-05 Alcon, Inc. RNAi-mediated inhibition of Frizzled Related Protein-1 for treatment of glaucoma
US9040494B2 (en) 2005-03-11 2015-05-26 Novartis Ag RNAi-mediated inhibition of frizzled related protein-1 for treatment of glaucoma
US7947660B2 (en) * 2005-03-11 2011-05-24 Alcon, Inc. RNAi-mediated inhibition of frizzled related protein-1 for treatment of glaucoma
US8173617B2 (en) 2005-03-11 2012-05-08 Novartis Ag RNAi-mediated inhibition of frizzled related protein-1 for treatment of glaucoma
US8999943B2 (en) * 2005-03-14 2015-04-07 Board Of Regents, The University Of Texas System Antigene oligomers inhibit transcription
US20060205635A1 (en) * 2005-03-14 2006-09-14 Board Of Regents, The University Of Texas System Antigene oligomers inhibit transcription
US20090123572A1 (en) * 2005-03-18 2009-05-14 Shiseido Company, Ltd. Method and Pharmaceutical Composition for Treating Psoriasis, Squamous Cell Carcinoma and/or Parakeratosis by Inhibiting Expression of Squamous Cell Carcinoma-Related Antigen
US7829547B2 (en) * 2005-03-18 2010-11-09 Shiseido Company, Ltd. Method and pharmaceutical composition for treating psoriasis, squamous cell carcinoma and/or parakeratosis by inhibiting expression of squamous cell carcinoma-related antigen
US20090203055A1 (en) * 2005-04-18 2009-08-13 Massachusetts Institute Of Technology Compositions and methods for RNA interference with sialidase expression and uses thereof
US8258112B2 (en) 2005-05-06 2012-09-04 Medtronic, Inc Methods and sequences to suppress primate huntington gene Expression
US7902352B2 (en) 2005-05-06 2011-03-08 Medtronic, Inc. Isolated nucleic acid duplex for reducing huntington gene expression
US20070160586A1 (en) * 2005-06-15 2007-07-12 Children's Medical Center Corporation Methods for extending the replicative lifespan of cells
US7838503B2 (en) * 2005-06-15 2010-11-23 Children's Medical Center Corporation Methods for extending the replicative lifespan of cells
US20110038922A1 (en) * 2005-06-16 2011-02-17 Faron Pharmaceuticals Oy (A Finnish Company) Compounds for treating or preventing amine oxidase related diseases or disorders
US7868159B2 (en) * 2005-06-23 2011-01-11 Baylor College Of Medicine Modulation of negative immune regulators and applications for immunotherapy
US20060292119A1 (en) * 2005-06-23 2006-12-28 Baylor College Of Medicine Modulation of negative immune regulators and applications for immunotherapy
US20100292305A1 (en) * 2005-06-27 2010-11-18 Akin Akinc RNAi MODULATION OF HIF-1 AND THERAPUTIC USES THEREOF
US9133517B2 (en) 2005-06-28 2015-09-15 Medtronics, Inc. Methods and sequences to preferentially suppress expression of mutated huntingtin
US8067573B2 (en) * 2005-07-07 2011-11-29 Yissum Research Development Company Of The Hebrew University Of Jerusalem Nucleic acid agents for downregulating H19 and methods of using same
US20090203121A1 (en) * 2005-07-07 2009-08-13 Avraham Hochberg Nucleic acid agents for downregulating h19, and methods of using same
US20090143321A1 (en) * 2005-07-07 2009-06-04 Avraham Hochberg Nucleic acid agents for downregulating h19 and methods of using same
US8067574B2 (en) 2005-07-07 2011-11-29 Yissum Research Development Company Of The Hebrew University Of Jerusalem Nucleic acid agents for downregulating H19, and methods of using same
US20080176293A1 (en) * 2005-07-25 2008-07-24 Jacques Rohayem RNA-Dependent RNA Polymerase, Methods And Kits For The Amplification And/Or Labelling Of RNA
US20070111227A1 (en) * 2005-07-28 2007-05-17 Green Pamela J Small regulatory RNAs and methods of use
US20100267810A1 (en) * 2005-08-18 2010-10-21 University Of Massachusetts Methods and compositions for treating neurological disease
US9914924B2 (en) 2005-08-18 2018-03-13 University Of Massachusetts Methods and compositions for treating neurological disease
US20070054873A1 (en) * 2005-08-26 2007-03-08 Protiva Biotherapeutics, Inc. Glucocorticoid modulation of nucleic acid-mediated immune stimulation
US8309533B2 (en) 2005-09-30 2012-11-13 University Of Massachusetts Allele-specific RNA interference
US20090149377A1 (en) * 2005-09-30 2009-06-11 St. Jude Children's Research Hospital METHODS FOR REGULATION OF p53 TRANSLATION AND FUNCTION
US8933043B2 (en) * 2005-09-30 2015-01-13 St. Jude Children's Research Hospital Methods for regulation of p53 translation and function
US20110160286A1 (en) * 2005-09-30 2011-06-30 University Of Massachusetts Allele-specific rna interference
WO2007047692A3 (en) * 2005-10-19 2007-08-02 Medtronic Inc Devices, systems and methods for improving memory and/or cognitive function through brain delivery of sirna
US20070135370A1 (en) * 2005-10-20 2007-06-14 Protiva Biotherapeutics, Inc. siRNA silencing of filovirus gene expression
US20110177131A1 (en) * 2005-10-20 2011-07-21 Protiva Biotherapeutics, Inc. siRNA SILENCING OF FILOVIRUS GENE EXPRESSION
US8354385B2 (en) 2005-10-20 2013-01-15 Sylentis S.A.U. Modulation of TRPV expression levels
US20100286230A1 (en) * 2005-10-20 2010-11-11 Sylentis S.A.U. Modulation of trpv expression levels
US7838658B2 (en) * 2005-10-20 2010-11-23 Ian Maclachlan siRNA silencing of filovirus gene expression
US8188057B2 (en) 2005-10-25 2012-05-29 Sylentis S.A.U. Modulation of 11beta-hydroxysteriod dehydrogenase 1 expression for the treatment of ocular diseases
US20110160277A1 (en) * 2005-10-25 2011-06-30 Sylentis S.A.U. Modulation of 11 beta-hydroxysteriod dehydrogenase 1 expression for the treatment of ocular diseases
US8080532B2 (en) 2005-10-28 2011-12-20 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of Huntingtin gene
US8314075B2 (en) 2005-10-28 2012-11-20 Alynylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of huntingtin gene
US20100298405A1 (en) * 2005-10-28 2010-11-25 Dinah Wen-Yee Sah Compositions And Methods For Inhibiting Expression Of Huntingtin Gene
US8101741B2 (en) * 2005-11-02 2012-01-24 Protiva Biotherapeutics, Inc. Modified siRNA molecules and uses thereof
US8513403B2 (en) 2005-11-02 2013-08-20 Protiva Biotherapeutics, Inc. Modified siRNA molecules and uses thereof
US8188263B2 (en) 2005-11-02 2012-05-29 Protiva Biotherapeutics, Inc. Modified siRNA molecules and uses thereof
US9074208B2 (en) 2005-11-02 2015-07-07 Protiva Biotherapeutics, Inc. Modified siRNA molecules and uses thereof
US20070135372A1 (en) * 2005-11-02 2007-06-14 Protiva Biotherapeutics, Inc. Modified siRNA molecules and uses thereof
US7902168B2 (en) 2005-11-04 2011-03-08 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of Nav1.8 gene
US20110124711A1 (en) * 2005-11-04 2011-05-26 Alnylam Pharmaceuticals, Inc. COMPOSITIONS AND METHODS FOR INHIBITING EXPRESSION OF Nav1.8 GENE
US20090258934A1 (en) * 2005-11-04 2009-10-15 Alnylam Pharmaceuticals, Inc. COMPOSITIONS AND METHODS FOR INHIBITING EXPRESSION OF Nav1.8 GENE
US20100129429A1 (en) * 2005-11-07 2010-05-27 British Columbia Cancer Agency Inhibition of autophagy genes in cancer chemotherapy
US8076308B2 (en) * 2005-11-07 2011-12-13 British Columbia Cancer Agency Inhibition of autophagy genes in cancer chemotherapy
US20110130443A1 (en) * 2005-11-09 2011-06-02 Hans-Peter Vornlocher Compositions And Methods For Inhibiting Expression Of Factor V Leiden Mutant Gene
US10501740B2 (en) 2005-11-09 2019-12-10 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of factor V
US9441225B2 (en) 2005-11-09 2016-09-13 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of factor V
US20100069461A1 (en) * 2005-11-09 2010-03-18 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of factor v leiden mutant gene
US8658782B2 (en) 2005-11-09 2014-02-25 Alynylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of factor V
US20070111963A1 (en) * 2005-11-17 2007-05-17 Board Of Regents, The University Of Texas System Modulation of gene expression by oligomers targeted to chromosomal DNA
US8324181B2 (en) 2005-11-17 2012-12-04 Board Of Regents, The University Of Texas System Modulation of gene expression by oligomers targeted to chromosomal DNA
US7709456B2 (en) 2005-11-17 2010-05-04 Board Of Regents, The University Of Texas System Modulation of gene expression by oligomers targeted to chromosomal DNA
US20080125384A1 (en) * 2005-11-21 2008-05-29 Shuewi Yang Simultaneous silencing and restoration of gene function
US20120202215A1 (en) * 2005-11-24 2012-08-09 Jichi Medical University Mitochondrial function of prohibitin 2 (phb2)
US8288354B2 (en) * 2005-12-28 2012-10-16 The Scripps Research Institute Natural antisense and non-coding RNA transcripts as drug targets
US9803195B2 (en) 2005-12-28 2017-10-31 The Scripps Research Institute Natural antisense and non-coding RNA transcripts as drug targets
US10472627B2 (en) 2005-12-28 2019-11-12 The Scripps Research Institute Natural antisense and non-coding RNA transcripts as drug targets
US20090156531A1 (en) * 2005-12-30 2009-06-18 Institut Gustave Roussy Use of Inhibitors of Scinderin and/or Ephrin-A1 for Treating Tumors
US8058252B2 (en) * 2005-12-30 2011-11-15 Institut Gustave Roussy Use of inhibitors of scinderin and/or ephrin-A1 for treating tumors
US20070259827A1 (en) * 2006-01-25 2007-11-08 University Of Massachusetts Compositions and methods for enhancing discriminatory RNA interference
US20070180242A1 (en) * 2006-01-30 2007-08-02 Nagaraj Thadi M GSM authentication in a CDMA network
US7956177B2 (en) * 2006-03-24 2011-06-07 Alnylam Pharmaceuticals, Inc. dsRNA compositions and methods for treating HPV infection
US20090247607A1 (en) * 2006-03-24 2009-10-01 John Benson dsRNA COMPOSITIONS AND METHODS FOR TREATING HPV INFECTION
US20110230546A1 (en) * 2006-03-24 2011-09-22 John Benson dsRNA COMPOSITIONS AND METHODS FOR TREATING HPV INFECTION
US20070238691A1 (en) * 2006-03-29 2007-10-11 Senesco Technologies, Inc. Inhibition of HIV replication and expression of p24 with eIF-5A
US9057069B2 (en) 2006-03-31 2015-06-16 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of Eg5 gene
US20090226446A1 (en) * 2006-04-06 2009-09-10 Deutsches Krebsforschungszentrum Stiftung Des Offentilchen Rechts Method to Inhibit the Propagation of an Undesired Cell Population
US20070275913A1 (en) * 2006-04-12 2007-11-29 Monia Brett P Compositions and their uses directed to hepcidin
US8076306B2 (en) * 2006-04-12 2011-12-13 Isis Pharmaceuticals, Inc. Compositions and their uses directed to hepcidin
US8058257B2 (en) 2006-04-28 2011-11-15 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of a gene from the JC virus
WO2007127919A3 (en) * 2006-04-28 2008-10-30 Alnylam Pharmaceuticals Inc Compositions and methods for inhibiting expression of a gene from the jc virus
US20090062225A1 (en) * 2006-04-28 2009-03-05 Pamela Tan Compositions and methods for inhibiting expression of a gene from the jc virus
US20100227915A1 (en) * 2006-04-28 2010-09-09 Pamela Tan Compositions and methods for inhibiting expression of a gene from the jc virus
US9012624B2 (en) 2006-04-28 2015-04-21 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of a gene from the JC virus
US7691824B2 (en) 2006-04-28 2010-04-06 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of a gene from the JC virus
US8410261B2 (en) 2006-04-28 2013-04-02 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of a gene from the JC virus
US8404832B2 (en) 2006-05-04 2013-03-26 Novartis Ag Short interfering ribonucleic acid (siRNA) for oral administration
US8404831B2 (en) 2006-05-04 2013-03-26 Novartis Ag Short interfering ribonucleic acid (siRNA) for oral administration
US9493771B2 (en) 2006-05-04 2016-11-15 Novartis Ag Short interfering ribonucleic acid (siRNA) for oral administration
US8957041B2 (en) 2006-05-04 2015-02-17 Novartis Ag Short interfering ribonucleic acid (siRNA) for oral administration
US20100015707A1 (en) * 2006-05-04 2010-01-21 Francois Jean-Charles Natt SHORT INTERFERING RIBONUCLEIC ACID (siRNA) FOR ORAL ADMINISTRATION
US8344128B2 (en) 2006-05-04 2013-01-01 Novartis Ag Short interfering ribonucleic acid (siRNA) for oral administration
US8084600B2 (en) 2006-05-04 2011-12-27 Novartis Ag Short interfering ribonucleic acid (siRNA) with improved pharmacological properties
US10501742B2 (en) 2006-05-11 2019-12-10 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of the PCSK9 gene
US9822365B2 (en) 2006-05-11 2017-11-21 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of the PCSK9 gene
US8222222B2 (en) 2006-05-11 2012-07-17 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of the PCSK9 gene
US9260718B2 (en) 2006-05-11 2016-02-16 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of the PCSK9 gene
US20110230542A1 (en) * 2006-05-11 2011-09-22 Pamela Tan Compositions and Methods for Inhibiting Expression of the PCSK9 Gene
US8809292B2 (en) 2006-05-11 2014-08-19 Alnylam Pharmaceuticals, Inc Compositions and methods for inhibiting expression of the PCSK9 gene
US20090130212A1 (en) * 2006-05-15 2009-05-21 Physical Pharmaceutica, Llc Composition and improved method for preparation of small particles
US20070269892A1 (en) * 2006-05-18 2007-11-22 Nastech Pharmaceutical Company Inc. FORMULATIONS FOR INTRACELLULAR DELIVERY dsRNA
WO2007137237A3 (en) * 2006-05-19 2008-12-18 Scripps Research Inst Treatment of protein misfolding
US20110003882A1 (en) * 2006-05-19 2011-01-06 Alnylam Pharmaceuticals, Inc. RNAi Modulation of AHA and Therapeutic Uses Thereof
US20080014191A1 (en) * 2006-05-19 2008-01-17 The Scripps Research Institute Treatment of Protein Misfolding
US8114984B2 (en) 2006-05-19 2012-02-14 Alnylam Pharmaceuticals, Inc. RNAi modulation of Aha and therapeutic uses thereof
US20080108584A1 (en) * 2006-05-22 2008-05-08 De Fougerolles Antonin Compositions and methods for inhibiting expression of ikk-b gene
US7888498B2 (en) * 2006-05-22 2011-02-15 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of IKK-B gene
US20110112178A1 (en) * 2006-05-22 2011-05-12 Alnylam Pharmaceuticals, Inc. Compositions And Methods For Inhibiting Expression Of IKK-B Gene
US9000143B2 (en) * 2006-05-22 2015-04-07 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of IKK-B gene
US9273356B2 (en) 2006-05-24 2016-03-01 Medtronic, Inc. Methods and kits for linking polymorphic sequences to expanded repeat mutations
US20070275923A1 (en) * 2006-05-25 2007-11-29 Nastech Pharmaceutical Company Inc. CATIONIC PEPTIDES FOR siRNA INTRACELLULAR DELIVERY
US20090275635A1 (en) * 2006-05-26 2009-11-05 Medical Research Council Screening method
US8598333B2 (en) 2006-05-26 2013-12-03 Alnylam Pharmaceuticals, Inc. SiRNA silencing of genes expressed in cancer
US20090149403A1 (en) * 2006-05-26 2009-06-11 Protiva Biotherapeutics, Inc. siRNA silencing of genes expressed in cancer
US9074202B2 (en) * 2006-05-26 2015-07-07 Medical Research Council Method of inhibiting human Trabid
US20080249046A1 (en) * 2006-06-09 2008-10-09 Protiva Biotherapeutics, Inc. MODIFIED siRNA MOLECULES AND USES THEREOF
US7915399B2 (en) 2006-06-09 2011-03-29 Protiva Biotherapeutics, Inc. Modified siRNA molecules and uses thereof
US20080152654A1 (en) * 2006-06-12 2008-06-26 Exegenics, Inc., D/B/A Opko Health, Inc. COMPOSITIONS AND METHODS FOR siRNA INHIBITION OF ANGIOGENESIS
US20100035966A1 (en) * 2006-06-14 2010-02-11 Rosetta Inpharmatics Llc Methods and compositions for regulating cell cycle progression
US9200275B2 (en) * 2006-06-14 2015-12-01 Merck Sharp & Dohme Corp. Methods and compositions for regulating cell cycle progression
WO2008002678A3 (en) * 2006-06-29 2008-10-16 Univ Texas Structural-based inhibitors of the glutathione binding site in aldose reductase, methods of screening therefor and methods of use
US20100016405A1 (en) * 2006-07-10 2010-01-21 Alnylam Pharmaceuticals, Inc Compositions and Methods for Inhibiting Expression of the MYC Gene
US8124752B2 (en) 2006-07-10 2012-02-28 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of the MYC gene
US20080057062A1 (en) * 2006-07-18 2008-03-06 National Institute Of Advanced Industrial Science And Technology Agent for Inducing senescence and apoptosis of cancer cell
US20080039415A1 (en) * 2006-08-11 2008-02-14 Gregory Robert Stewart Retrograde transport of sirna and therapeutic uses to treat neurologic disorders
US20100330105A1 (en) * 2006-08-22 2010-12-30 John Hopkins University Anticancer Combination Therapies
US20090061487A1 (en) * 2006-09-08 2009-03-05 Samuel Jotham Reich Sirna and methods of manufacture
US7872118B2 (en) 2006-09-08 2011-01-18 Opko Ophthalmics, Llc siRNA and methods of manufacture
US20110143400A1 (en) * 2006-09-08 2011-06-16 Opko Ophthalmics, Llc Sirna and methods of manufacture
US9090895B2 (en) 2006-09-21 2015-07-28 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of the HAMP gene
US9506067B2 (en) 2006-09-21 2016-11-29 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of the HAMP gene
US8163711B2 (en) 2006-09-21 2012-04-24 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of the HAMP gene
US8268799B2 (en) 2006-09-21 2012-09-18 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of the HAMP gene
US20100204307A1 (en) * 2006-09-21 2010-08-12 Tomoko Nakayama Compositions And Methods For Inhibiting Expression Of The HAMP Gene
US8791250B2 (en) 2006-09-21 2014-07-29 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of the HAMP gene
US8470799B2 (en) 2006-09-21 2013-06-25 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of the HAMP gene
US20110150897A1 (en) * 2006-10-11 2011-06-23 Meyer Thomas F Influenza targets
US20080279844A1 (en) * 2006-10-18 2008-11-13 Kapil Mehta Methods for Treating Cancer Targeting Transglutaminase
US8299040B2 (en) * 2006-10-18 2012-10-30 Board Of Regents, The University Of Texas System Methods for treating cancer targeting transglutaminase
US9375440B2 (en) 2006-11-03 2016-06-28 Medtronic, Inc. Compositions and methods for making therapies delivered by viral vectors reversible for safety and allele-specificity
US8324367B2 (en) 2006-11-03 2012-12-04 Medtronic, Inc. Compositions and methods for making therapies delivered by viral vectors reversible for safety and allele-specificity
US8034921B2 (en) 2006-11-21 2011-10-11 Alnylam Pharmaceuticals, Inc. IRNA agents targeting CCR5 expressing cells and uses thereof
US7819842B2 (en) 2006-11-21 2010-10-26 Medtronic, Inc. Chronically implantable guide tube for repeated intermittent delivery of materials or fluids to targeted tissue sites
US7988668B2 (en) 2006-11-21 2011-08-02 Medtronic, Inc. Microsyringe for pre-packaged delivery of pharmaceuticals
US20080255345A1 (en) * 2006-11-21 2008-10-16 Alnylam Pharmaceuticals, Inc. IRNA Agents Targeting CCR5 Expressing Cells And Uses Thereof
US20080171719A1 (en) * 2006-11-28 2008-07-17 Alcon Manufacturing, Ltd. RNAi-MEDIATED INHIBITION OF AQUAPORIN 1 FOR TREATMENT OF IOP-RELATED CONDITIONS
US7754698B2 (en) * 2007-01-09 2010-07-13 Isis Pharmaceuticals, Inc. Modulation of FR-alpha expression
US20080167265A1 (en) * 2007-01-09 2008-07-10 Isis Pharmaceuticals Inc Modulation of fr-alpha expression
US7928083B2 (en) 2007-01-16 2011-04-19 Yissum Research Development Company Of The Hebrew University Of Jerusalem H19 silencing nucleic acid agents for treating rheumatoid arthritis
US20080171906A1 (en) * 2007-01-16 2008-07-17 Everaerts Frank J L Tissue performance via hydrolysis and cross-linking
WO2008088836A3 (en) * 2007-01-16 2008-10-16 Burnham Inst Medical Research Compositions and methods for treatment of colorectal cancer
US9260715B2 (en) 2007-01-16 2016-02-16 The University Of Queensland Method of inducing an immune response
US20100105759A1 (en) * 2007-01-16 2010-04-29 Abraham Hochberg H19 silencing nucleic acid agents for treating rheumatoid arthritis
US20090074785A1 (en) * 2007-01-16 2009-03-19 Smith Jeffrey W Compositions and methods for treatment of colorectal cancer
US20100196403A1 (en) * 2007-01-29 2010-08-05 Jacob Hochman Antibody conjugates for circumventing multi-drug resistance
US20100183696A1 (en) * 2007-01-30 2010-07-22 Allergan, Inc Treating Ocular Diseases Using Peroxisome Proliferator-Activated Receptor Delta Antagonists
US8729042B2 (en) * 2007-01-30 2014-05-20 Allergan, Inc. Treating ocular diseases using peroxisome proliferator—activated receptor delta antagonists
US20120136041A1 (en) * 2007-01-30 2012-05-31 Allergan, Inc. Treating Ocular Diseases Using Peroxisome Proliferator-Activated Receptor Delta Antagonists
US20080299659A1 (en) * 2007-03-02 2008-12-04 Nastech Pharmaceutical Company Inc. Nucleic acid compounds for inhibiting apob gene expression and uses thereof
US9085638B2 (en) 2007-03-07 2015-07-21 The Johns Hopkins University DNA vaccine enhancement with MHC class II activators
US20080260765A1 (en) * 2007-03-15 2008-10-23 Johns Hopkins University HPV DNA Vaccines and Methods of Use Thereof
US20100249052A1 (en) * 2007-03-26 2010-09-30 Alnylam Pharmaceuticals, Inc. Dsrna compositions and methods for treating hpv infections
US8354390B2 (en) 2007-03-29 2013-01-15 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of a gene from the ebola virus
US7973020B2 (en) 2007-03-29 2011-07-05 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of a gene from the ebola virus
US9187516B2 (en) 2007-03-29 2015-11-17 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of a gene from the Ebola virus
US8735369B2 (en) 2007-03-29 2014-05-27 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of a gene from the Ebola virus
US20110201671A1 (en) * 2007-03-29 2011-08-18 Alnylam Pharmaceuticals, Inc. Compositions And Methods For Inhibiting Expression Of A Gene From The Ebola Virus
US20110060031A1 (en) * 2007-03-29 2011-03-10 Alnylam Pharmaceuticals, Inc. Compositions And Methods For Inhibiting Expression Of A Gene From The Ebola Virus
US20090203135A1 (en) * 2007-04-23 2009-08-13 Alnylam Pharmaceuticals, Inc. Glycoconjugates of RNA Interference Agents
US10131907B2 (en) 2007-04-23 2018-11-20 Alnylam Pharmaceuticals, Inc. Glycoconjugates of RNA interference agents
US8877917B2 (en) * 2007-04-23 2014-11-04 Alnylam Pharmaceuticals, Inc. Glycoconjugates of RNA interference agents
US9873875B2 (en) 2007-05-01 2018-01-23 City Of Hope Methods and compositions for the specific inhibition of gene expression by double-stranded RNA
US20100151470A1 (en) * 2007-05-01 2010-06-17 University Of Massachusetts Methods and compositions for locating snp heterozygosity for allele specific diagnosis and therapy
US10233450B2 (en) 2007-05-01 2019-03-19 City Of Hope Methods and compositions for the specific inhibition of gene expression by double-stranded RNA
US20090029466A1 (en) * 2007-05-01 2009-01-29 City Of Hope Methods and compositions for the specific inhibition of gene expression by double-stranded rna
US8883996B2 (en) 2007-05-01 2014-11-11 City Of Hope Methods and compositions for the specific inhibition of gene expression by double-stranded RNA
US20100240734A1 (en) * 2007-05-01 2010-09-23 City Of Hope Methods and compositions for the specific inhibition of gene expression by double-stranded rna
US9441227B2 (en) 2007-05-01 2016-09-13 City Of Hope Methods and compositions for the specific inhibition of gene expression by double-stranded RNA
US20090022667A1 (en) * 2007-05-15 2009-01-22 Marco Peters METHODS OF TREATING COGNITIVE DISORDERS BY INHIBITION OF Gpr12
US7968524B2 (en) * 2007-05-15 2011-06-28 Helicon Therapeutics, Inc. Methods of enhancing long term memory formation by inhibition of Gpr12
US9297009B2 (en) 2007-05-22 2016-03-29 Arcturus Therapeutics, Inc. UNA oligomers targeting micro-RNA for therapeutics
US9051570B2 (en) 2007-05-22 2015-06-09 Arcturus Therapeutics, Inc. UNA oligomers for therapeutics
US9944929B2 (en) 2007-05-22 2018-04-17 Arcturus Therapeutics, Inc. UNA single stranded oligomers for therapeutics
US9303260B2 (en) 2007-05-22 2016-04-05 Arcturus Therapeutics, Inc. UNA duplex oligomers for therapeutics
US10457945B2 (en) 2007-05-22 2019-10-29 Arcturus Therapeutics, Inc. UNA oligomers for therapeutics with prolonged stability
US9914927B2 (en) 2007-06-15 2018-03-13 Arrowhead Pharmaceuticals, Inc. RNAi inhibition of alpha-ENaC expression
US8168606B2 (en) * 2007-06-15 2012-05-01 Novartis Ag RNAi inhibition of alpha-ENaC expression
US7943592B2 (en) * 2007-06-15 2011-05-17 Novartis Ag RNAi inhibition of alpha-ENaC expression
US20100216971A1 (en) * 2007-06-15 2010-08-26 Novartis Ag RNAi Inhibition of Alpha-ENaC Expression
US9476052B2 (en) 2007-06-15 2016-10-25 Arrowhead Pharmaceuticals, Inc. RNAi inhibition of alpha-ENaC expression
US8119612B2 (en) * 2007-06-15 2012-02-21 Novartis Ag RNAi inhibition of alpha-ENaC expression
US20100216972A1 (en) * 2007-06-15 2010-08-26 Novartis Ag RNAi Inhibition of Alpha-ENaC Expression
US20100210514A1 (en) * 2007-06-15 2010-08-19 Novartis Ag RNAi Inhibition of Alpha-ENaC Expression
US20100210513A1 (en) * 2007-06-15 2010-08-19 Novartis Ag RNAi Inhibition of Alpha-ENaC Expression
US9074212B2 (en) * 2007-06-15 2015-07-07 Arrowhead Research Corporation RNAi inhibition of alpha-ENaC expression
US20100190714A1 (en) * 2007-06-15 2010-07-29 Novatis Ag RNAi Inhibition of Alpha-ENaC Expression
US11208662B2 (en) 2007-06-15 2021-12-28 Arrowhead Pharmaceuticals, Inc. RNAi inhibition of alpha-ENaC expression
US7939508B2 (en) * 2007-06-15 2011-05-10 Novartis Ag RNAi inhibition of alpha-ENaC expression
US10544418B2 (en) 2007-06-15 2020-01-28 Arrowhead Pharmaceuticals, Inc. RNAi inhibition of alpha-ENaC expression
US10689650B2 (en) 2007-06-29 2020-06-23 Stelic Institute & Co. Method of fixing and expressing physiologically active substance
US20140135379A1 (en) * 2007-06-29 2014-05-15 Niigata University Method of fixing and expressing physiologically active substance
US11485974B2 (en) 2007-06-29 2022-11-01 Stelic Institute & Co. Method of fixing and expressing physiologically active substance
US20110172286A1 (en) * 2007-07-10 2011-07-14 Neurim Pharmaceuticals (1991) Ltd. Cd44 splice variants in neurodegenerative diseases
US9416166B2 (en) 2007-07-10 2016-08-16 Neurim Pharmaceuticals (1991) Ltd. CD44 splice variants in neurodegenerative diseases
US9018180B2 (en) * 2007-07-10 2015-04-28 Neurim Pharmaceuticals (1991) Ltd. CD44 splice variants in neurodegenerative diseases
US20090053298A1 (en) * 2007-07-31 2009-02-26 Sumitomo Chemical Company, Limited Compositions and methods for inhibiting car gene expression by rna interference
US7816338B2 (en) * 2007-07-31 2010-10-19 Sumitomo Chemical Company, Limited Compositions and methods for inhibiting car gene expression by RNA interference
US20110184041A1 (en) * 2007-08-17 2011-07-28 Richard Kremer PTHrP, ITS ISOFORMS AND ANTAGONIST THERETO IN THE DIAGNOSIS AND TREATMENT OF DISEASE
US8501929B2 (en) * 2007-08-17 2013-08-06 Biochrom Pharma Inc. PTHrP, its isoforms and antagonist thereto in the diagnosis and treatment of disease
US20090081789A1 (en) * 2007-08-31 2009-03-26 Greenville Hospital System Activation of nuclear factor kappa B
US9080215B2 (en) 2007-09-14 2015-07-14 Asuragen, Inc. MicroRNAs differentially expressed in cervical cancer and uses thereof
US8361714B2 (en) 2007-09-14 2013-01-29 Asuragen, Inc. Micrornas differentially expressed in cervical cancer and uses thereof
US20100240441A1 (en) * 2007-09-14 2010-09-23 Konami Digital Entertainment Co., Ltd Game system, and game apparatus and challenge notifying apparatus constituting the game system
US20100136026A1 (en) * 2007-09-26 2010-06-03 Kerr William G Ship Inhibition to Direct Hematopoietic Stem Cells and Induce Extramedullary Hematopoiesis
US20090092988A1 (en) * 2007-10-04 2009-04-09 Schwartz Jacob C Modulating Gene Expression with agRNA and Gapmers Targeting Antisense Transcripts
US8097712B2 (en) 2007-11-07 2012-01-17 Beelogics Inc. Compositions for conferring tolerance to viral disease in social insects, and the use thereof
US20090118214A1 (en) * 2007-11-07 2009-05-07 Beeologics, Llc Compositions for conferring tolerance to viral disease in social insects, and the use thereof
US8507457B2 (en) 2007-11-07 2013-08-13 Beeologics Inc. Compositions for conferring tolerance to viral disease in social insects, and the use thereof
US10888579B2 (en) 2007-11-07 2021-01-12 Beeologics Inc. Compositions for conferring tolerance to viral disease in social insects, and the use thereof
US20100098664A1 (en) * 2007-11-28 2010-04-22 Mathieu Jean-Francois Desclaux Lentiviral vectors allowing RNAi mediated inhibition of GFAP and vimentin expression
US20100255023A1 (en) * 2007-11-30 2010-10-07 Si-Yi Chen Dendritic cell vaccine compositions and uses of same
US8324369B2 (en) 2007-11-30 2012-12-04 Baylor College Of Medicine Dendritic cell vaccine compositions and uses of same
US8071562B2 (en) 2007-12-01 2011-12-06 Mirna Therapeutics, Inc. MiR-124 regulated genes and pathways as targets for therapeutic intervention
US11110174B2 (en) 2007-12-04 2021-09-07 Alnylam Pharmaceuticals, Inc. Carbohydrate conjugates as delivery agents for oligonucleotides
US9867882B2 (en) 2007-12-04 2018-01-16 Alnylam Pharmaceuticals, Inc. Carbohydrate conjugates as delivery agents for oligonucleotides
US8507455B2 (en) 2007-12-04 2013-08-13 Alnylam Pharmaceuticals, Inc. Folate conjugates
US8828956B2 (en) 2007-12-04 2014-09-09 Alnylam Pharmaceuticals, Inc. Carbohydrate conjugates as delivery agents for oligonucleotides
US9370582B2 (en) 2007-12-04 2016-06-21 Alnylam Pharmaceuticals, Inc. Carbohydrate conjugates as delivery agents for oligonucleotides
US9814777B2 (en) 2007-12-04 2017-11-14 Arbutus Biopharma Corporation Targeting lipids
US9370581B2 (en) 2007-12-04 2016-06-21 Alnylam Pharmaceuticals, Inc. Carbohydrate conjugates as delivery agents for oligonucleotides
US20090247614A1 (en) * 2007-12-04 2009-10-01 Alnylam Pharmaceuticals, Inc. Folate Conjugates
US9352048B2 (en) 2007-12-04 2016-05-31 Alnylam Pharmaceuticals, Inc. Carbohydrate conjugates as delivery agents for oligonucleotides
US8106022B2 (en) 2007-12-04 2012-01-31 Alnylam Pharmaceuticals, Inc. Carbohydrate conjugates as delivery agents for oligonucleotides
US11666653B2 (en) 2007-12-04 2023-06-06 Alnylam Pharmaceuticals, Inc. Carbohydrate conjugates as delivery agents for oligonucleotides
US20090239814A1 (en) * 2007-12-04 2009-09-24 Alnylam Pharmaceuticals, Inc. Carbohydrate Conjugates as Delivery Agents for Oligonucleotides
US8450467B2 (en) 2007-12-04 2013-05-28 Alnylam Pharmaceuticals, Inc. Carbohydrate conjugates as delivery agents for oligonucleotides
US20090247608A1 (en) * 2007-12-04 2009-10-01 Alnylam Pharmaceuticals, Inc. Targeting Lipids
US10806791B2 (en) 2007-12-04 2020-10-20 Alnylam Pharmaceuticals, Inc. Carbohydrate conjugates as delivery agents for oligonucleotides
US8334273B2 (en) 2007-12-10 2012-12-18 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of factor VII gene
US9062310B2 (en) 2007-12-10 2015-06-23 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of factor VII gene
US8664193B2 (en) 2007-12-10 2014-03-04 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of factor VII gene
US8410073B2 (en) 2007-12-13 2013-04-02 Alnylam Pharmaceuticals, Inc. Methods and compositions for prevention or treatment of RSV infection
US9127277B2 (en) 2007-12-13 2015-09-08 Alnylam Pharmaceuticals, Inc. Methods and compositions for prevention or treatment of RSV infection
US20090238772A1 (en) * 2007-12-13 2009-09-24 Alnylam Pharmaceuticals, Inc. Methods and compositions for prevention or treatment of rsv infection
US20100204306A1 (en) * 2007-12-14 2010-08-12 Alnylam Pharmaceuticals, Inc. Method of Treating Neurodegenerative Disease
US20100010066A1 (en) * 2008-01-31 2010-01-14 Kevin Fitzgerald Optimized Methods For Delivery Of DSRNA Targeting The PCSK9 Gene
US10633654B2 (en) 2008-02-11 2020-04-28 Phio Pharmaceuticals Corp. Modified RNAi polynucleotides and uses thereof
US10131904B2 (en) 2008-02-11 2018-11-20 Rxi Pharmaceuticals Corporation Modified RNAi polynucleotides and uses thereof
US8288525B2 (en) 2008-02-12 2012-10-16 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of CD45 gene
US20110034537A1 (en) * 2008-02-12 2011-02-10 De Fougerolles Antonin Compositions and methods for inhibiting expression of cd45 gene
US20090203138A1 (en) * 2008-02-12 2009-08-13 University Of Tennessee Research Foundation, Inc. Small Interfering RNAs Targeting Feline Herpes Virus
US7977321B2 (en) * 2008-02-12 2011-07-12 University Of Tennessee Research Foundation Small interfering RNAs targeting feline herpes virus
US8912316B2 (en) 2008-02-12 2014-12-16 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of CD45 gene
US20110207796A1 (en) * 2008-02-13 2011-08-25 Elan Pharma International Limited Alpha-synuclein kinase
US20100087508A1 (en) * 2008-03-05 2010-04-08 David Bumcrot Compositions and Methods for Inhibiting Expression of Eg5 and VEGF Genes
US9006197B2 (en) 2008-03-05 2015-04-14 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of Eg5 and VEGF genes
US8148344B2 (en) 2008-03-27 2012-04-03 Alnylam Pharmaceuticals, Inc. Compositions and methods for mediating RNAi in vivo
US20100003317A1 (en) * 2008-03-27 2010-01-07 Akin Akinc Compositions and methods for mediating rnai in vivo
US20080221059A1 (en) * 2008-04-03 2008-09-11 National Taiwan University Novel treatment tool for cancer: rna interference of bcas2
US7718631B2 (en) * 2008-04-03 2010-05-18 National Taiwan University Treatment tool for cancer: RNA interference of BCAS2
US8822668B2 (en) 2008-04-15 2014-09-02 Protiva Biotherapeutics, Inc. Lipid formulations for nucleic acid delivery
US8492359B2 (en) 2008-04-15 2013-07-23 Protiva Biotherapeutics, Inc. Lipid formulations for nucleic acid delivery
US9364435B2 (en) 2008-04-15 2016-06-14 Protiva Biotherapeutics, Inc. Lipid formulations for nucleic acid delivery
US8058069B2 (en) 2008-04-15 2011-11-15 Protiva Biotherapeutics, Inc. Lipid formulations for nucleic acid delivery
US11141378B2 (en) 2008-04-15 2021-10-12 Arbutus Biopharma Corporation Lipid formulations for nucleic acid delivery
US20090285861A1 (en) * 2008-04-17 2009-11-19 Tzyy-Choou Wu Tumor cell-based cancer immunotherapeutic compositions and methods
US8344126B2 (en) 2008-04-17 2013-01-01 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of XBP-1 gene
US8765932B2 (en) 2008-04-17 2014-07-01 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of XBP-1 gene
US20090275638A1 (en) * 2008-04-17 2009-11-05 Kevin Fitzgerald Compositions and Methods for Inhibiting Expression of XBP-1 Gene
US7875711B2 (en) 2008-04-17 2011-01-25 Alnylam Pharamaceuticals, Inc. Compositions and methods for inhibiting expression of XBP-1 gene
US20110152350A1 (en) * 2008-04-17 2011-06-23 Kevin Fitzgerald Compositions and Methods for Inhibiting Expression of XBP-1 Gene
USRE48948E1 (en) 2008-04-18 2022-03-01 Warsaw Orthopedic, Inc. Clonidine compounds in a biodegradable polymer
US8324366B2 (en) 2008-04-29 2012-12-04 Alnylam Pharmaceuticals, Inc. Compositions and methods for delivering RNAI using lipoproteins
US9365852B2 (en) 2008-05-08 2016-06-14 Mirna Therapeutics, Inc. Compositions and methods related to miRNA modulation of neovascularization or angiogenesis
US9540645B2 (en) 2008-05-08 2017-01-10 The John Hopkins University Compositions and methods related to miRNA modulation of neovascularization or angiogenesis
US8258111B2 (en) 2008-05-08 2012-09-04 The Johns Hopkins University Compositions and methods related to miRNA modulation of neovascularization or angiogenesis
US8222221B2 (en) 2008-06-04 2012-07-17 The Board Of Regents Of The University Of Texas System Modulation of gene expression through endogenous small RNA targeting of gene promoters
US8431692B2 (en) 2008-06-06 2013-04-30 Quark Pharmaceuticals, Inc. Compositions and methods for treatment of ear disorders
US20110142917A1 (en) * 2008-06-06 2011-06-16 Egvenia Alpert Compositions and methods for treatment of ear disorders
US9089591B2 (en) 2008-06-06 2015-07-28 Quark Pharmaceuticals, Inc. Compositions and methods for treatment of ear disorders
US20110053226A1 (en) * 2008-06-13 2011-03-03 Riboxx Gmbh Method for enzymatic synthesis of chemically modified rna
US20110184046A1 (en) * 2008-07-11 2011-07-28 Dinah Wen-Yee Sah Compositions And Methods For Inhibiting Expression Of GSK-3 Genes
US9029525B2 (en) 2008-07-11 2015-05-12 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of GSK-3 genes
US8815818B2 (en) 2008-07-18 2014-08-26 Rxi Pharmaceuticals Corporation Phagocytic cell delivery of RNAI
US9089610B2 (en) 2008-08-19 2015-07-28 Nektar Therapeutics Complexes of small-interfering nucleic acids
US9433684B2 (en) 2008-08-19 2016-09-06 Nektar Therapeutics Conjugates of small-interfering nucleic acids
US9096851B2 (en) 2008-08-25 2015-08-04 Excaliard Pharmaceuticals, Inc. Antisense oligonucleotides directed against connective tissue growth factor and uses thereof
US20190323013A1 (en) * 2008-08-25 2019-10-24 Excaliard Pharmaceuticals, Inc. Antisense oligonucleotides directed against connective tissue growth factor and uses thereof
US9688987B2 (en) 2008-08-25 2017-06-27 Excaliard Pharmaceuticals, Inc. Antisense oligonucleotides directed against connective tissue growth factor and uses thereof
US8252762B2 (en) * 2008-08-25 2012-08-28 Excaliard Pharmaceuticals, Inc. Antisense oligonucleotides directed against connective tissue growth factor and uses thereof
US8772260B2 (en) 2008-08-25 2014-07-08 Isis Pharmaceuticals, Inc Methods for inhibiting expression of connective tissue growth factor
US20100130595A1 (en) * 2008-08-25 2010-05-27 Dean Nicholas M Antisense oligonucleotides directed against connective tissue growth factor and uses thereof
US10570392B2 (en) 2008-09-15 2020-02-25 The Children's Medical Center Corporation Modulation of BCL11A for treatment of hemoglobinopathies
US10266826B2 (en) 2008-09-15 2019-04-23 Children's Medical Center Corporation Modulation of BCL11A for treatment of hemoglobinopathies
US9885041B2 (en) * 2008-09-15 2018-02-06 The Children's Medical Center Corporation Modulation of BCL11A for treatment of hemoglobinopathies
US20170152513A1 (en) * 2008-09-15 2017-06-01 Children's Medical Center Corporation Modulation of bcl11a for treatment of hemoglobinopathies
US10774330B2 (en) 2008-09-22 2020-09-15 Phio Pharmaceuticals Corp. Reduced size self-delivering RNAI compounds
US10041073B2 (en) 2008-09-22 2018-08-07 Rxi Pharmaceuticals Corporation Reduced size self-delivering RNAi compounds
US10138485B2 (en) 2008-09-22 2018-11-27 Rxi Pharmaceuticals Corporation Neutral nanotransporters
US9938530B2 (en) 2008-09-22 2018-04-10 Rxi Pharmaceuticals Corporation RNA interference in skin indications
US8796443B2 (en) 2008-09-22 2014-08-05 Rxi Pharmaceuticals Corporation Reduced size self-delivering RNAi compounds
US10815485B2 (en) 2008-09-22 2020-10-27 Phio Pharmaceuticals Corp. RNA interference in skin indications
US10876119B2 (en) 2008-09-22 2020-12-29 Phio Pharmaceuticals Corp. Reduced size self-delivering RNAI compounds
US9303259B2 (en) 2008-09-22 2016-04-05 Rxi Pharmaceuticals Corporation RNA interference in skin indications
US9175289B2 (en) 2008-09-22 2015-11-03 Rxi Pharmaceuticals Corporation Reduced size self-delivering RNAi compounds
US11396654B2 (en) 2008-09-22 2022-07-26 Phio Pharmaceuticals Corp. Neutral nanotransporters
US8664189B2 (en) 2008-09-22 2014-03-04 Rxi Pharmaceuticals Corporation RNA interference in skin indications
US8546554B2 (en) 2008-09-25 2013-10-01 Alnylam Pharmaceuticals, Inc. Lipid formulated compositions and methods for inhibiting expression of Serum Amyloid A gene
US9868950B2 (en) 2008-09-25 2018-01-16 Alnylam Pharmaceuticals, Inc. Lipid formulated compositions and methods for inhibiting expression of serum amyloid A gene
US9206421B2 (en) 2008-09-25 2015-12-08 Alnylam Pharmaceuticals, Inc. Lipid formulated compositions and methods for inhibiting expression of serum amyloid A gene
US11884919B2 (en) 2008-09-25 2024-01-30 Alnylam Pharmaceuticals, Inc. Lipid formulated compositions and methods for inhibiting expression of serum amyloid a gene
US10472628B2 (en) 2008-09-25 2019-11-12 Alnylam Pharmaceuticals, Inc. Lipid formulated compositions and methods for inhibiting expression of Serum Amyloid A gene
US11149273B2 (en) 2008-09-25 2021-10-19 Alnylam Pharmaceuticals, Inc. Lipid formulated compositions and methods for inhibiting expression of serum amyloid A gene
US20140051747A1 (en) * 2008-10-06 2014-02-20 Alnylam Pharmaceuticals, Inc. Compositions and Methods for Inhibiting Expression of an RNA from West Nile Virus
US9200282B2 (en) * 2008-10-06 2015-12-01 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of an RNA from west nile virus
US8592570B2 (en) 2008-10-06 2013-11-26 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of an RNA from West Nile virus
US10653780B2 (en) 2008-10-09 2020-05-19 The University Of British Columbia Amino lipids and methods for the delivery of nucleic acids
US9139554B2 (en) 2008-10-09 2015-09-22 Tekmira Pharmaceuticals Corporation Amino lipids and methods for the delivery of nucleic acids
US8779115B2 (en) 2008-10-15 2014-07-15 Somagenics Inc. Short hairpin RNAs for inhibition of gene expression
US20100112686A1 (en) * 2008-10-15 2010-05-06 Qing Ge Short hairpin rnas for inhibition of gene expression
US8283460B2 (en) 2008-10-15 2012-10-09 Somagenics, Inc. Short hairpin RNAs for inhibition of gene expression
US20100120893A1 (en) * 2008-10-20 2010-05-13 Dinah Wen-Yee Sah Compositions and Methods for Inhibiting Expression of Transthyretin
US8741866B2 (en) 2008-10-20 2014-06-03 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of transthyretin
US8168775B2 (en) 2008-10-20 2012-05-01 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of transthyretin
US9234196B2 (en) 2008-10-20 2016-01-12 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of transthyretin
US10240152B2 (en) 2008-10-20 2019-03-26 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of transthyretin
US20100168205A1 (en) * 2008-10-23 2010-07-01 Alnylam Pharmaceuticals, Inc. Methods and Compositions for Prevention or Treatment of RSV Infection Using Modified Duplex RNA Molecules
US20110190380A1 (en) * 2008-10-23 2011-08-04 Elena Feinstein Methods for delivery of sirna to bone marrow cells and uses thereof
US11254940B2 (en) 2008-11-19 2022-02-22 Phio Pharmaceuticals Corp. Inhibition of MAP4K4 through RNAi
US9074211B2 (en) 2008-11-19 2015-07-07 Rxi Pharmaceuticals Corporation Inhibition of MAP4K4 through RNAI
US8470792B2 (en) 2008-12-04 2013-06-25 Opko Pharmaceuticals, Llc. Compositions and methods for selective inhibition of VEGF
US20100291188A1 (en) * 2008-12-04 2010-11-18 Musc Foundation For Research Development Periostin Inhibitory Compositions for Myocardial Regeneration, Methods of Delivery, and Methods of Using Same
US8889644B2 (en) 2008-12-10 2014-11-18 Alnylam Pharmaceuticals, Inc. GNAQ targeted dsRNA compositions and methods for inhibiting expression
US20100168206A1 (en) * 2008-12-10 2010-07-01 Jared Gollob GNAQ Targeted dsRNA Compositions And Methods For Inhibiting Expression
US8324368B2 (en) 2008-12-10 2012-12-04 Alnylam Pharmaceuticals, Inc. GNAQ targeted dsRNA compositions and methods for inhibiting expression
US10954516B2 (en) 2008-12-10 2021-03-23 Alnylam Pharmaceuticals, Inc. GNAQ targeted dsRNA compositions and methods for inhibiting expression
US9963700B2 (en) 2008-12-10 2018-05-08 Alnylam Pharmaceuticals, Inc. GNAQ targeted dsRNA compositions and methods for inhibiting expression
US9566295B2 (en) 2008-12-10 2017-02-14 Alnylam Pharmaceuticals, Inc. GNAQ targeted dsRNA compositions and methods for inhibiting expression
US20100166661A1 (en) * 2008-12-11 2010-07-01 Bojian Zheng siRNA COMPOSITIONS AND METHODS FOR POTENTLY INHIBITING VIRAL INFECTION
US8664188B2 (en) * 2008-12-11 2014-03-04 Xiangxue Group (Hong Kong) Company Limited siRNA compositions and methods for potently inhibiting viral infection
US20110245325A1 (en) * 2008-12-12 2011-10-06 Kureha Corporation Pharmaceutical composition for treatment of cancer and asthma
US8389711B2 (en) * 2008-12-12 2013-03-05 Kureha Corporation Pharmaceutical composition for treatment of cancer and asthma
US9493774B2 (en) 2009-01-05 2016-11-15 Rxi Pharmaceuticals Corporation Inhibition of PCSK9 through RNAi
US10167471B2 (en) 2009-01-05 2019-01-01 Rxi Pharmaceuticals Corporation Inhibition of PCSK9 through RNAI
US9428751B2 (en) 2009-01-26 2016-08-30 Protiva Biotherapeutics, Inc. Compositions and methods for silencing apolipoprotein C-III expression
US9023820B2 (en) 2009-01-26 2015-05-05 Protiva Biotherapeutics, Inc. Compositions and methods for silencing apolipoprotein C-III expression
US20100197773A1 (en) * 2009-02-03 2010-08-05 Birgit Bramlage Compositions and methods for inhibiting expression of ptp1b genes
US11667915B2 (en) 2009-02-04 2023-06-06 Phio Pharmaceuticals Corp. RNA duplexes with single stranded phosphorothioate nucleotide regions for additional functionality
US9745574B2 (en) 2009-02-04 2017-08-29 Rxi Pharmaceuticals Corporation RNA duplexes with single stranded phosphorothioate nucleotide regions for additional functionality
US10479992B2 (en) 2009-02-04 2019-11-19 Phio Pharmaceuticals Corp. RNA duplexes with single stranded phosphorothioate nucleotide regions for additional functionality
US9993567B2 (en) 2009-03-23 2018-06-12 Quark Pharmaceuticals, Inc. Composition of anti-ENDO180 antibodies and methods of use for the treatment of cancer and fibrotic diseases
US8444983B2 (en) 2009-03-23 2013-05-21 Quark Pharmaceuticals, Inc. Composition of anti-ENDO180 antibodies and methods of use for the treatment of cancer and fibrotic diseases
US10653619B2 (en) 2009-03-23 2020-05-19 Medtronic, Inc. Drug depots for treatment of pain and inflammation
US20100273863A1 (en) * 2009-04-24 2010-10-28 Board Of Regents, The University Of Texas System Modulation of Gene Expression Using Oligomers That Target Gene Regions Downstream of 3' Untranslated Regions
US8815586B2 (en) 2009-04-24 2014-08-26 The Board Of Regents Of The University Of Texas System Modulation of gene expression using oligomers that target gene regions downstream of 3′ untranslated regions
US9932579B2 (en) 2009-05-05 2018-04-03 Beeologics Inc Prevention and treatment of Nosema disease in bees
US20140057960A1 (en) * 2009-05-05 2014-02-27 Medical Diagnostic Laboratories, Llc Novel repressor on ifn-lambda promoter and sirna against zeb1 and blimp-1 to increase ifn-lambda gene activity
US8822426B2 (en) 2009-05-05 2014-09-02 Beeologics Inc. Prevention and treatment of nosema disease in bees
US8933049B2 (en) * 2009-05-05 2015-01-13 Medical Diagnostic Laboratories, Llc Repressor on IFN-λ promoter and siRNA against ZEB1 and BLIMP-1 to increase IFN-λ gene activity
US20110020300A1 (en) * 2009-05-15 2011-01-27 Genentech, Inc. Compositions and methods for inhibiting expression of glucocorticoid receptor (gcr) genes
US9200276B2 (en) 2009-06-01 2015-12-01 Halo-Bio Rnai Therapeutics, Inc. Polynucleotides for multivalent RNA interference, compositions and methods of use thereof
US9957505B2 (en) 2009-06-01 2018-05-01 Halo-Bio Rnai Therapeutics, Inc. Polynucleotides for multivalent RNA interference, compositions and methods of use thereof
US10053689B2 (en) 2009-06-15 2018-08-21 Arbutus Biopharma Corporation Methods for increasing efficacy of lipid formulated siRNA
US8598139B2 (en) 2009-06-15 2013-12-03 Alnylam Pharmaceuticals, Inc. Lipid formulated dsRNA targeting the PCSK9 gene
US20110015252A1 (en) * 2009-06-15 2011-01-20 Kevin Fitzgerald Lipid formulated dsrna targeting the pcsk9 gene
US8273869B2 (en) 2009-06-15 2012-09-25 Alnylam Pharmaceuticals, Inc. Lipid formulated dsRNA targeting the PCSK9 gene
US9051567B2 (en) 2009-06-15 2015-06-09 Tekmira Pharmaceuticals Corporation Methods for increasing efficacy of lipid formulated siRNA
US8569256B2 (en) 2009-07-01 2013-10-29 Protiva Biotherapeutics, Inc. Cationic lipids and methods for the delivery of therapeutic agents
US9018187B2 (en) 2009-07-01 2015-04-28 Protiva Biotherapeutics, Inc. Cationic lipids and methods for the delivery of therapeutic agents
US11786598B2 (en) 2009-07-01 2023-10-17 Arbutus Biopharma Corporation Lipid formulations for delivery of therapeutic agents
US11446383B2 (en) 2009-07-01 2022-09-20 Arbutus Biopharma Corporation Lipid formulations for delivery of therapeutic agents
US8283333B2 (en) 2009-07-01 2012-10-09 Protiva Biotherapeutics, Inc. Lipid formulations for nucleic acid delivery
US9878042B2 (en) 2009-07-01 2018-01-30 Protiva Biotherapeutics, Inc. Lipid formulations for delivery of therapeutic agents to solid tumors
US9816091B2 (en) 2009-07-13 2017-11-14 Somagenics, Inc. Chemical modification of short small hairpin RNAs for inhibition of gene expression
US8871730B2 (en) 2009-07-13 2014-10-28 Somagenics Inc. Chemical modification of short small hairpin RNAs for inhibition of gene expression
US10870850B2 (en) 2009-07-13 2020-12-22 Somagenics, Inc. Chemical modification of short small hairpin RNAs for inhibition of gene expression
US20110201667A1 (en) * 2009-07-20 2011-08-18 Protiva Biotherapeutics, Inc. Compositions and methods for silencing ebola virus gene expression
US9187748B2 (en) 2009-07-20 2015-11-17 Protiva Biotherapeutics, Inc. Compositions and methods for silencing ebola virus gene expression
US8716464B2 (en) 2009-07-20 2014-05-06 Thomas W. Geisbert Compositions and methods for silencing Ebola virus gene expression
US9029338B2 (en) 2009-08-14 2015-05-12 Alnylam Pharmaceuticals, Inc. Lipid formulated compositions and methods for inhibiting expression of a gene from the ebola virus
US8859516B2 (en) 2009-09-15 2014-10-14 Alnylam Pharmaceuticals, Inc. Lipid formulated compositions and methods for inhibiting expression of Eg5 and VEGF genes
WO2011035065A1 (en) 2009-09-17 2011-03-24 Nektar Therapeutics Monoconjugated chitosans as delivery agents for small interfering nucleic acids
US8916693B2 (en) 2009-09-17 2014-12-23 Nektar Therapeutics Monoconjugated chitosans as delivery agents for small interfering nucleic acids
US9187746B2 (en) 2009-09-22 2015-11-17 Alnylam Pharmaceuticals, Inc. Dual targeting siRNA agents
US9222086B2 (en) * 2009-09-23 2015-12-29 Protiva Biotherapeutics, Inc. Compositions and methods for silencing genes expressed in cancer
US20130065939A1 (en) * 2009-09-23 2013-03-14 Protiva Biotherapeutics, Inc. Compositions and methods for silencing genes expressed in cancer
US10801028B2 (en) 2009-10-14 2020-10-13 Beeologics Inc. Compositions for controlling Varroa mites in bees
US8962584B2 (en) 2009-10-14 2015-02-24 Yissum Research Development Company Of The Hebrew University Of Jerusalem, Ltd. Compositions for controlling Varroa mites in bees
US9662348B2 (en) 2009-10-14 2017-05-30 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Compositions for controlling Varroa mites in bees
US9101643B2 (en) 2009-11-03 2015-08-11 Alnylam Pharmaceuticals, Inc. Lipid formulated compositions and methods for inhibiting expression of transthyretin (TTR)
US20110110483A1 (en) * 2009-11-06 2011-05-12 Searete Llc, A Limited Liability Corporation Of The State Of Delaware Methods and systems for migrating fuel assemblies in a nuclear fission reactor
US20110166199A1 (en) * 2010-01-07 2011-07-07 National Cheng Kung University RNAi COMPOUND TARGETED TO THROMBOSPONDIN-1 AND APPLICATIONS THEREOF
US8377902B2 (en) * 2010-01-07 2013-02-19 National Cheng Kung University RNAi compound targeted to thrombospondin-1 and applications thereof
US8722641B2 (en) 2010-01-29 2014-05-13 St. Jude Children's Research Hospital Oligonucleotides which inhibit p53 induction in response to cellular stress
US9540647B2 (en) 2010-03-19 2017-01-10 University Of South Alabama Methods and compositions for the treatment of cancer
US9186370B2 (en) * 2010-03-19 2015-11-17 University Of South Alabama Methods and compositions for the treatment of cancer
US20130224311A1 (en) * 2010-03-19 2013-08-29 University Of South Alabama Methods and compositions for the treatment of cancer
US9080171B2 (en) 2010-03-24 2015-07-14 RXi Parmaceuticals Corporation Reduced size self-delivering RNAi compounds
US10662430B2 (en) 2010-03-24 2020-05-26 Phio Pharmaceuticals Corp. RNA interference in ocular indications
US9963702B2 (en) 2010-03-24 2018-05-08 Rxi Pharmaceuticals Corporation RNA interference in dermal and fibrotic indications
US11118178B2 (en) 2010-03-24 2021-09-14 Phio Pharmaceuticals Corp. Reduced size self-delivering RNAI compounds
US10184124B2 (en) 2010-03-24 2019-01-22 Phio Pharmaceuticals Corp. RNA interference in ocular indications
US10913948B2 (en) 2010-03-24 2021-02-09 Phio Pharmaceuticals Corp. RNA interference in dermal and fibrotic indications
US9095504B2 (en) 2010-03-24 2015-08-04 Rxi Pharmaceuticals Corporation RNA interference in ocular indications
US11584933B2 (en) 2010-03-24 2023-02-21 Phio Pharmaceuticals Corp. RNA interference in ocular indications
US9340786B2 (en) 2010-03-24 2016-05-17 Rxi Pharmaceuticals Corporation RNA interference in dermal and fibrotic indications
US10240149B2 (en) 2010-03-24 2019-03-26 Phio Pharmaceuticals Corp. Reduced size self-delivering RNAi compounds
US8455455B1 (en) 2010-03-31 2013-06-04 Protiva Biotherapeutics, Inc. Compositions and methods for silencing genes involved in hemorrhagic fever
US20140010893A1 (en) * 2010-05-12 2014-01-09 University Of South Carolina Methods for Affecting Homology-Directed DNA Double Stranded Break Repair
US9095561B2 (en) * 2010-05-12 2015-08-04 University Of South Carolina Methods for affecting homology-directed DNA double stranded break repair
US9518272B2 (en) 2010-06-30 2016-12-13 Protiva Biotherapeutics, Inc. Non-liposomal systems for nucleic acid delivery
US9006417B2 (en) 2010-06-30 2015-04-14 Protiva Biotherapeutics, Inc. Non-liposomal systems for nucleic acid delivery
US9404127B2 (en) 2010-06-30 2016-08-02 Protiva Biotherapeutics, Inc. Non-liposomal systems for nucleic acid delivery
US11718852B2 (en) 2010-06-30 2023-08-08 Arbutus Biopharma Corporation Non-liposomal systems for nucleic acid delivery
US9642872B2 (en) 2010-09-30 2017-05-09 University Of Zurich Treatment of B-cell lymphoma with microRNA
US20140315973A1 (en) * 2010-10-07 2014-10-23 Agency For Science, Technology And Research Parp-1 inhibitors
US9260471B2 (en) 2010-10-29 2016-02-16 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using short interfering nucleic acids (siNA)
US11932854B2 (en) 2010-10-29 2024-03-19 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using short interfering nucleic acids (siNA)
US9970005B2 (en) 2010-10-29 2018-05-15 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using short interfering nucleic acids (siNA)
US11193126B2 (en) 2010-10-29 2021-12-07 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using short interfering nucleic acids (siNA)
US9173894B2 (en) 2011-02-02 2015-11-03 Excaliard Pharamaceuticals, Inc. Method of treating keloids or hypertrophic scars using antisense compounds targeting connective tissue growth factor (CTGF)
US9611478B2 (en) 2011-02-03 2017-04-04 Mirna Therapeutics, Inc. Synthetic mimics of miR-124
US9233121B2 (en) * 2011-03-11 2016-01-12 Board Of Regents Of The University Of Nebraska Compositions and methods for the treatment of cancer
US20140057962A1 (en) * 2011-03-11 2014-02-27 Surinder K. Batra Compositions and Methods for the Treatment of Cancer
US9068184B2 (en) 2011-06-21 2015-06-30 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibition of expression of protein C (PROC) genes
US11834662B2 (en) 2011-06-21 2023-12-05 Alnylam Pharmaceuticals, Inc. Angiopoietin-like 3 (ANGPTL3) iRNA compositions and methods of use thereof
US11306315B2 (en) 2011-06-21 2022-04-19 Alnylam Pharmaceuticals, Inc. Angiopoietin-like 3 (ANGPTL3) iRNA compositions and methods of use thereof
US11306316B2 (en) 2011-06-21 2022-04-19 Alnylam Pharmaceuticals, Inc. Angiopoietin-like 3 (ANGPTL3) iRNA compositions and methods of use thereof
US20140179768A1 (en) * 2011-06-21 2014-06-26 Alnylam Pharmaceuticals, Inc. ANGIOPOIETIN-LIKE 3 (ANGPTL3) iRNA COMPOSITIONS AND METHODS OF USE THEREOF
US9970006B2 (en) 2011-06-21 2018-05-15 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibition of expression of apolipoprotein C-III (APOC3) genes
US10557139B2 (en) 2011-06-21 2020-02-11 Alnylam Pharmaceuticals, Inc. Angiopoietin-like 3 (ANGPTL3) iRNA compositions and methods of use thereof
US10550390B2 (en) 2011-06-21 2020-02-04 Alnylam Pharmaceuticals, Inc. Angiopoietin-like 3 (ANGPTL3) iRNA compositions and methods of use thereof
US10337010B2 (en) 2011-06-21 2019-07-02 Alnylam Pharmaceuticals, Inc. Angiopoietin-like 3 (ANGPTL3) iRNA compositions and methods of use thereof
US11130953B2 (en) 2011-06-21 2021-09-28 Alnylam Pharmaceuticals, Inc. Angiopoietin-like 3 (ANGPTL3) iRNA compositions and methods of use thereof
US11866709B2 (en) 2011-06-21 2024-01-09 Alnylam Pharmaceuticals, Inc. Angiopoietin-like 3 (ANGPTL3) iRNA compositions and methods of use thereof
US11306314B2 (en) 2011-06-21 2022-04-19 Alnylam Pharmaceuticals, Inc. Angiopoietin-like 3 (ANGPTL3) iRNA compositions and methods of use thereof
US9725718B2 (en) 2011-06-21 2017-08-08 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibition of expression of protein C (PROC) genes
US9315813B2 (en) 2011-06-21 2016-04-19 Alnylam Pharmaceuticals, Inc Compositions and methods for inhibition of expression of apolipoprotein C-III (APOC3) genes
US11332743B2 (en) 2011-06-21 2022-05-17 Alnylam Pharmaceuticals, Inc. Angiopoietin-like 3 (ANGPTL3) iRNA compositions and methods of use thereof
US9228188B2 (en) 2011-06-21 2016-01-05 Alnylam Pharmaceuticals, Inc. Compositions and method for inhibiting hepcidin antimicrobial peptide (HAMP) or HAMP-related gene expression
US9322018B2 (en) * 2011-06-21 2016-04-26 Alnylam Pharmaceuticals, Inc. Angiopoietin-like 3 (ANGPTL3) iRNA compositions and methods of use thereof
US9771591B2 (en) 2011-06-21 2017-09-26 Alnylam Pharmaceuticals, Inc. Angiopoietin-like 3 (ANGPTL3) iRNA compositions and methods of use thereof
US11525138B2 (en) 2011-06-21 2022-12-13 Alnylam Pharmaceuticals, Inc. Angiopoietin-like 3 (ANGPTL3) iRNA compositions and methods of use thereof
US10273478B2 (en) 2011-06-21 2019-04-30 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibition of expression of protein C (PROC) genes
US10934545B2 (en) 2011-06-21 2021-03-02 Alnylam Pharmaceuticals, Inc. Angiopoietin-like 3 (ANGPTL3) iRNA compositions and methods of use thereof
US11118181B2 (en) 2011-06-21 2021-09-14 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibition of expression of protein C (PROC) genes
US11840692B2 (en) 2011-06-21 2023-12-12 Alnylam Pharmaceuticals, Inc. Angiopoietin-like 3 (ANGPTL3) iRNA compositions and methods of use thereof
DE102011118024A1 (de) 2011-08-01 2013-02-07 Technische Universität Dresden Inhibitor der Expression der Pro-Caspase 1
US10655184B2 (en) 2011-09-13 2020-05-19 Interpace Diagnostics, Llc Methods and compositions involving miR-135b for distinguishing pancreatic cancer from benign pancreatic disease
US9644241B2 (en) 2011-09-13 2017-05-09 Interpace Diagnostics, Llc Methods and compositions involving miR-135B for distinguishing pancreatic cancer from benign pancreatic disease
US10570391B2 (en) 2011-11-18 2020-02-25 Alnylam Pharmaceuticals, Inc. RNAi agents, compositions and methods of use thereof for treating transthyretin (TTR) associated diseases
US9399775B2 (en) 2011-11-18 2016-07-26 Alnylam Pharmaceuticals, Inc. RNAi agents, compositions and methods of use thereof for treating transthyretin (TTR) associated diseases
US20140364485A1 (en) * 2012-01-06 2014-12-11 University Of South Alabama Methods and compositions for the treatment of cancer
US9464291B2 (en) * 2012-01-06 2016-10-11 University Of South Alabama Methods and compositions for the treatment of cancer
US10214745B2 (en) 2012-03-15 2019-02-26 The Scripps Research Institute Treatment of brain derived neurotrophic factor (BDNF) related diseases by inhibition of natural antisense transcript to BDNF
US10011832B2 (en) 2012-09-05 2018-07-03 Sylentis Sau SiRNA and their use in methods and compositions for the treatment and/or prevention of eye conditions
US9808479B2 (en) 2012-09-05 2017-11-07 Sylentis Sau SiRNA and their use in methods and compositions for the treatment and / or prevention of eye conditions
US20150202223A1 (en) * 2012-10-02 2015-07-23 The General Hospital Corporation Methods relating to dna-sensing pathway related conditions
US9814741B2 (en) * 2012-10-02 2017-11-14 The General Hospital Corporation Methods relating to DNA-sensing pathway related conditions
US11542493B2 (en) 2012-11-27 2023-01-03 The Children's Medical Center Corporation Targeting BCL11A distal regulatory elements for fetal hemoglobin reinduction
US20160215285A1 (en) * 2012-12-30 2016-07-28 The Regents Of The University Of California Methods of modulating compliance of the trabecular meshwork
US9920320B2 (en) * 2012-12-30 2018-03-20 The Regents Of The University Of California Methods of modulating compliance of the trabecular meshwork
US20160032402A1 (en) * 2013-03-15 2016-02-04 Novartis Ag Biomarkers associated with brm inhibition
US20160024504A1 (en) * 2013-03-15 2016-01-28 Constellation Pharmaceuticals, Inc. Treating th2-mediated diseases by inhibition of bromodomains
US9850543B2 (en) * 2013-03-15 2017-12-26 Novartis Ag Biomarkers associated with BRM inhibition
US11377667B2 (en) 2013-07-19 2022-07-05 Monsanto Technology Llc Compositions and methods for controlling Leptinotarsa
US10597676B2 (en) 2013-07-19 2020-03-24 Monsanto Technology Llc Compositions and methods for controlling Leptinotarsa
US11053500B2 (en) * 2013-08-28 2021-07-06 lonis Pharmaceuticals, Inc. Modulation of prekallikrein (PKK) expression
US11840686B2 (en) 2013-08-28 2023-12-12 Ionis Pharmaceuticals, Inc. Modulation of prekallikrein (PKK) expression
US10934550B2 (en) 2013-12-02 2021-03-02 Phio Pharmaceuticals Corp. Immunotherapy of cancer
US10011837B2 (en) 2014-03-04 2018-07-03 Sylentis Sau SiRNAs and their use in methods and compositions for the treatment and/or prevention of eye conditions
US9856475B2 (en) 2014-03-25 2018-01-02 Arcturus Therapeutics, Inc. Formulations for treating amyloidosis
US10683500B2 (en) 2014-03-25 2020-06-16 Arcturus Therapeutics, Inc. UNA oligomers having reduced off-target effects in gene silencing
US9982259B2 (en) 2014-03-25 2018-05-29 Arcturus Therapeutics, Inc. Transthyretin allele selective UNA oligomers for gene silencing
US10604758B2 (en) 2014-03-25 2020-03-31 Arcturus Therapeutics, Inc. Therapeutic oligomers for treating amyloidosis
US11091770B2 (en) 2014-04-01 2021-08-17 Monsanto Technology Llc Compositions and methods for controlling insect pests
US11124794B2 (en) 2014-04-25 2021-09-21 The Children's Medical Center Corporation Compositions and methods to treating hemoglobinopathies
US10662429B2 (en) 2014-04-25 2020-05-26 The Children's Medical Center Corporation Compositions and methods to treating hemoglobinopathies
US10287588B2 (en) 2014-04-25 2019-05-14 The Childrens's Medical Center Corporation Compositions and methods to treating hemoglobinopathies
US11739329B2 (en) 2014-04-25 2023-08-29 The Children's Medical Center Corporation Compositions and methods to treating hemoglobinopathies
US11279934B2 (en) 2014-04-28 2022-03-22 Phio Pharmaceuticals Corp. Methods for treating cancer using nucleic acids targeting MDM2 or MYCN
US11613752B2 (en) 2014-05-01 2023-03-28 Ionis Pharmaceuticals, Inc. Compositions and methods for modulating PKK expression
US10378012B2 (en) 2014-07-29 2019-08-13 Monsanto Technology Llc Compositions and methods for controlling insect pests
US11124792B2 (en) 2014-07-29 2021-09-21 Monsanto Technology Llc Compositions and methods for controlling insect pests
US10060921B2 (en) 2014-08-29 2018-08-28 Alnylam Pharmaceuticals, Inc. Methods of treating transthyretin (TTR) mediated amyloidosis
US11079379B2 (en) 2014-08-29 2021-08-03 Alnylam Pharmaceuticals, Inc. Methods of treating transthyretin (TTR) mediated amyloidosis
US10900039B2 (en) 2014-09-05 2021-01-26 Phio Pharmaceuticals Corp. Methods for treating aging and skin disorders using nucleic acids targeting Tyr or MMP1
US11926828B2 (en) 2014-09-05 2024-03-12 Phio Pharmaceuticals Corp. Methods for treating aging and skin disorders using nucleic acids targeting TYR or MMP1
US11446380B2 (en) 2014-10-10 2022-09-20 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibition of HAO1 (hydroxyacid oxidase 1 (glycolate oxidase)) gene expression
US10478500B2 (en) 2014-10-10 2019-11-19 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibition of HAO1 (Hydroxyacid Oxidase 1 (Glycolate Oxidase)) gene expression
US10513703B2 (en) 2014-11-10 2019-12-24 Alnylam Pharmaceuticals, Inc. Hepatitis B virus (HBV) iRNA compositions and methods of use thereof
US11060091B2 (en) 2014-11-10 2021-07-13 Alnylam Pharmaceuticals, Inc. Hepatitis B virus (HBV) iRNA compositions and methods of use thereof
US11142766B2 (en) 2014-11-17 2021-10-12 Alnylam Pharmaceuticals, Inc. Apolipoprotein C3 (APOC3) iRNA compositions and methods of use thereof
US11408001B1 (en) 2014-11-17 2022-08-09 Alnylam Pharmaceuticals, Inc. Apolipoprotein C3 (APOC3) iRNA compositions and methods of use thereof
US10968449B2 (en) 2015-01-22 2021-04-06 Monsanto Technology Llc Compositions and methods for controlling Leptinotarsa
US10519447B2 (en) 2015-04-01 2019-12-31 Arcturus Therapeutics, Inc. Therapeutic UNA oligomers and uses thereof
US10570393B2 (en) 2015-04-13 2020-02-25 Alnylam Pharmaceuticals, Inc. Angiopoietin-like 3 (ANGPTL3) iRNA compositions and methods of use thereof
US11198872B2 (en) 2015-04-13 2021-12-14 Alnylam Pharmaceuticals, Inc. Angiopoietin-like 3 (ANGPTL3) iRNA compositions and methods of use thereof
US11572543B2 (en) 2015-05-08 2023-02-07 The Children's Medical Center. Corporation Targeting BCL11A enhancer functional regions for fetal hemoglobin reinduction
US10550387B2 (en) * 2015-06-30 2020-02-04 Chugai Seiyaku Kabushiki Kaisha Therapeutic agent for a lung disease and/or method for screening for the same
US20180195064A1 (en) * 2015-06-30 2018-07-12 Tadamitsu Kishimoto Novel therapeutic agent for a lung disease and/or method for screening for the same
US11279931B2 (en) 2015-06-30 2022-03-22 Tadamitsu Kishimoto Therapeutic agent for a lung disease and/or method for screening for the same
US11001845B2 (en) 2015-07-06 2021-05-11 Phio Pharmaceuticals Corp. Nucleic acid molecules targeting superoxide dismutase 1 (SOD1)
US10808247B2 (en) 2015-07-06 2020-10-20 Phio Pharmaceuticals Corp. Methods for treating neurological disorders using a synergistic small molecule and nucleic acids therapeutic approach
US11312962B2 (en) 2015-07-10 2022-04-26 Ionis Pharmaceuticals, Inc. Modulators of diacyglycerol acyltransferase 2 (DGAT2)
US10421964B2 (en) 2015-07-23 2019-09-24 Arcturus Therapeutics, Inc. UNA oligomers and compositions for treating amyloidosis
US10208307B2 (en) 2015-07-31 2019-02-19 Alnylam Pharmaceuticals, Inc. Transthyretin (TTR) iRNA compositions and methods of use thereof for treating or preventing TTR-associated diseases
US11286486B2 (en) 2015-07-31 2022-03-29 Alnylam Pharmaceuticals, Inc. Transthyretin (TTR) iRNA compositions and methods of use thereof for treating or preventing TTR-associated diseases
US10683501B2 (en) 2015-07-31 2020-06-16 Alnylam Pharmaceuticals, Inc. Transthyretin (TTR) iRNA compositions and methods of use thereof for treating or preventing TTR-associated diseases
US10731157B2 (en) 2015-08-24 2020-08-04 Halo-Bio Rnai Therapeutics, Inc. Polynucleotide nanoparticles for the modulation of gene expression and uses thereof
WO2017050848A1 (en) * 2015-09-21 2017-03-30 Oommen Oommen P Nucleic acid molecules with enhanced activity
US11021707B2 (en) 2015-10-19 2021-06-01 Phio Pharmaceuticals Corp. Reduced size self-delivering nucleic acid compounds targeting long non-coding RNA
US10246709B2 (en) 2016-03-07 2019-04-02 Arrowhead Pharmaceuticals, Inc. Targeting ligands for therapeutic compounds
US10294474B2 (en) 2016-09-02 2019-05-21 Arrowhead Pharmaceuticals, Inc. Targeting ligands
US11174481B2 (en) 2016-09-02 2021-11-16 Arrowhead Pharmaceuticals, Inc. Targeting ligands
US11723912B2 (en) 2016-12-08 2023-08-15 University Of Utah Research Foundation Staufen1 agents and associated methods
US11261441B2 (en) 2017-03-29 2022-03-01 Bluebird Bio, Inc. Vectors and compositions for treating hemoglobinopathies
US11324820B2 (en) 2017-04-18 2022-05-10 Alnylam Pharmaceuticals, Inc. Methods for the treatment of subjects having a hepatitis b virus (HBV) infection
US11788087B2 (en) 2017-05-25 2023-10-17 The Children's Medical Center Corporation BCL11A guide delivery
US11214802B2 (en) 2017-07-06 2022-01-04 Arrowhead Pharmaceuticals, Inc. RNAi agents for inhibiting expression of alpha-ENaC and methods of use
US10590416B2 (en) 2017-07-06 2020-03-17 Arrowhead Pharmaceuticals, Inc. RNAi agents for inhibiting expression of alpha-ENaC and methods of use
US11261447B2 (en) 2017-07-13 2022-03-01 Alnylam Pharmaceuticals, Inc. Methods for inhibition of HAO1 (hydroxyacid oxidase 1 (glycolate oxidase)) gene expression
US10597657B2 (en) 2017-09-11 2020-03-24 Arrowhead Pharmaceuticals, Inc. RNAi agents and compositions for inhibiting expression of apolipoprotein C-III (APOC3)
US11214801B2 (en) 2017-09-11 2022-01-04 Arrowhead Pharmaceuticals, Inc. RNAi agents and compositions for inhibiting expression of apolipoprotein C-III (APOC3)
US11806360B2 (en) 2017-09-19 2023-11-07 Alnylam Pharmaceuticals, Inc. Compositions and methods for treating transthyretin (TTR) mediated amyloidosis
US11739328B2 (en) 2017-12-01 2023-08-29 The Texas A&M University System Angleman syndrome antisense treatment
US11685920B2 (en) 2017-12-01 2023-06-27 The Texas A&M University System Angelman syndrome antisense treatment
US11198869B2 (en) * 2017-12-01 2021-12-14 The Texas A&M University System Angelman syndrome antisense treatment
US20210238590A1 (en) * 2018-06-14 2021-08-05 University Of Utah Research Foundation Staufen1 regulating agents and associated methods
US11946046B2 (en) * 2018-06-14 2024-04-02 University Of Utah Research Foundation Staufen1 regulating agents and associated methods
US11492623B2 (en) 2018-08-13 2022-11-08 Alnylam Pharmaceuticals, Inc. Hepatitis B virus (HBV) dsRNA agent compositions and methods of use thereof
US11591544B2 (en) 2020-11-25 2023-02-28 Akagera Medicines, Inc. Ionizable cationic lipids
US11613751B2 (en) 2021-03-04 2023-03-28 Alnylam Pharmaceuticals, Inc. Angiopoietin-like 3 (ANGPTL3) iRNA compositions and methods of use thereof
US11959081B2 (en) 2021-08-03 2024-04-16 Alnylam Pharmaceuticals, Inc. Transthyretin (TTR) iRNA compositions and methods of use thereof

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