BR0211111A - Molécula de ácido nucleico isolada, vetor, célula hospedeira, transgene, precursor de rna engenheirado, animal transgênico não humano, e, método de induzir a interferência de ácido ribonucleico de um gene alvo em uma célula - Google Patents

Molécula de ácido nucleico isolada, vetor, célula hospedeira, transgene, precursor de rna engenheirado, animal transgênico não humano, e, método de induzir a interferência de ácido ribonucleico de um gene alvo em uma célula

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Publication number
BR0211111A
BR0211111A BR0211111-0A BR0211111A BR0211111A BR 0211111 A BR0211111 A BR 0211111A BR 0211111 A BR0211111 A BR 0211111A BR 0211111 A BR0211111 A BR 0211111A
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Brazil
Prior art keywords
cell
engineered rna
nucleic acid
transgene
vector
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BR0211111-0A
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English (en)
Inventor
Phillip D Zamore
Gyorgy Hutvagner
Juanita Mclachlan
Craig C Mello
Alla Grishok
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Univ Massachusetts
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Application filed by Univ Massachusetts filed Critical Univ Massachusetts
Publication of BR0211111A publication Critical patent/BR0211111A/pt

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • C12N2310/00—Structure or type of the nucleic acid
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Abstract

"MOLéCULA DE áCIDO NUCLEICO ISOLADA, VETOR, CéLULA HOSPEDEIRA, TRANSGENE, PRECURSOR DE RNA ENGENHEIRADO, ANIMAL TRANSGêNICO NãO HUMANO, E, MéTODO DE INDUZIR A INTERFERêNCIA DE áCIDO RIBONUCLEICO DE UM GENE ALVO EM UMA CéLULA". A invenção fornece precursores de RNA engenheirados que quando expressados em uma célula são processados pela célula para produzir RNAs interferentes pequenos alvejados (siRNAs) que seletivamente silenciam genes alvejados (pela limpeza de mRNAs específicos) usando o caminho de interferência de RNA (RNAi) da própria célula. Introduzindo-se moléculas de ácido nucleico que codificam estes precursores de RNA engenheirados em células in vivo com seq³ências reguladoras apropriadas, a expressão dos precursores de RNA engenheirados pode ser seletivamente controlada tanto temporal quanto espacialmente, isto é, em tempos particulares e/ou em tecidos, órgãos ou células particulares.
BR0211111-0A 2001-07-12 2002-07-12 Molécula de ácido nucleico isolada, vetor, célula hospedeira, transgene, precursor de rna engenheirado, animal transgênico não humano, e, método de induzir a interferência de ácido ribonucleico de um gene alvo em uma célula BR0211111A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US30518501P 2001-07-12 2001-07-12
PCT/US2002/022010 WO2003006477A1 (en) 2001-07-12 2002-07-12 IN VIVO PRODUCTION OF SMALL INTERFERING RNAs THAT MEDIATE GENE SILENCING

Publications (1)

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BR0211111A true BR0211111A (pt) 2004-06-22

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BR0211111-0A BR0211111A (pt) 2001-07-12 2002-07-12 Molécula de ácido nucleico isolada, vetor, célula hospedeira, transgene, precursor de rna engenheirado, animal transgênico não humano, e, método de induzir a interferência de ácido ribonucleico de um gene alvo em uma célula

Country Status (9)

Country Link
US (8) US7691995B2 (pt)
EP (3) EP1412371B1 (pt)
JP (1) JP4210737B2 (pt)
KR (2) KR20040022449A (pt)
BR (1) BR0211111A (pt)
CA (2) CA2921821A1 (pt)
ES (3) ES2566561T3 (pt)
IL (3) IL159756A0 (pt)
WO (1) WO2003006477A1 (pt)

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