DK2494075T3 - Skabelonstyrede nanokonjugater - Google Patents

Skabelonstyrede nanokonjugater Download PDF

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DK2494075T3
DK2494075T3 DK10827627.0T DK10827627T DK2494075T3 DK 2494075 T3 DK2494075 T3 DK 2494075T3 DK 10827627 T DK10827627 T DK 10827627T DK 2494075 T3 DK2494075 T3 DK 2494075T3
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nanoconjugate
polynucleotide
nanoparticle
biomolecule
biomolecules
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DK10827627.0T
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Chad A Mirkin
David A Giljohann
Joshua I Cutler
Ke Zhang
Dan Zheng
Weston L Daniel
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Univ Northwestern
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    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/69—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit
    • A61K47/6921—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a particulate, a powder, an adsorbate, a bead or a sphere
    • A61K47/6923—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a particulate, a powder, an adsorbate, a bead or a sphere the form being an inorganic particle, e.g. ceramic particles, silica particles, ferrite or synsorb
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • A61K47/02—Inorganic compounds
    • A—HUMAN NECESSITIES
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    • A—HUMAN NECESSITIES
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    • A61K49/0069—Preparation for luminescence or biological staining characterised by a special physical or galenical form, e.g. emulsions, microspheres the agent being in a particular physical galenical form
    • A61K49/0089—Particulate, powder, adsorbate, bead, sphere
    • A61K49/0091—Microparticle, microcapsule, microbubble, microsphere, microbead, i.e. having a size or diameter higher or equal to 1 micrometer
    • A61K49/0093—Nanoparticle, nanocapsule, nanobubble, nanosphere, nanobead, i.e. having a size or diameter smaller than 1 micrometer, e.g. polymeric nanoparticle
    • A—HUMAN NECESSITIES
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    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
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    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
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Claims (23)

1. Sammensætning, der omfatter et nanokonjugat, hvilket nanokonjugat har en defineret struktur og omfatter en flerhed af biomolekyler, der er krydsbundet til hinanden i et monomolekylært lag, en overflade, der tilvejebringer en skabelon, hvorpå strukturen samles, hvor hvert biomolekyle omfatter en alkyndel, hvor overfladen omfatter guld og eventuelt fjernes mindst delvist, efter at strukturen er blevet defineret, og, hvor overfladen er en katalysator for krydsbindingen.
2. Sammensætning ifølge krav 1, hvor overfladen er en nanopartikel.
3. Sammensætning ifølge krav 2, hvor nanopartiklen er valgt fra gruppen, der består af en kugle, en stav og en prisme.
4. Sammensætning ifølge et hvilket som helst af ovennævnte krav, hvor biomolekylet er valgt fra gruppen, der består af et polynukleotid, et peptid, et polypeptid, et phospholipid, et oligosaccharid, et lille molekyle, et terapeutisk middel, et kontrastmiddel og kombinationer deraf.
5. Sammensætning ifølge et hvilket som helst af ovennævnte krav, hvor flerheden af biomolekyler er monodispers.
6. Sammensætning ifølge et hvilket som helst af ovennævnte krav, hvor monodispersiteten er således, at der er ca. 25 % variation i diameteren af flerheden af nanokonjugater.
7. Sammensætning ifølge et hvilket som helst af ovennævnte krav, hvor flerheden af nanokonjugater yderligere omfatter et ekstra middel valgt fra gruppen, der består af et polynukleotid, et peptid, et polypeptid, et phospholipid, et oligosaccharid, et metalkompleks, et lille molekyle, et terapeutisk middel, et kontrastmiddel og kombinationer deraf.
8. Sammensætning ifølge et hvilket som helst af ovennævnte krav, hvor mindst ét nanokonjugat i flerheden af nanokonjugater er hult i fravær af overfladen.
9. Sammensætning ifølge krav 8, hvor et ekstra middel er indkapslet i nanokonjugatet, der ellers er hult.
10. Fremgangsmåde til krydsbinding af et struktureret nanokonjugat, hvilken fremgangsmåde omfatter trinet aktivering af et første biomolekyle ved etablering af kontakt mellem det første biomolekyle og en overflade, hvor aktiveringen åbner mulighed for, at det første biomolekyle kan krydsbinde til et andet biomolekyle, hvor det første biomolekyle og det andet biomolekyle hver omfatter mindst én alkyndel.
11. Fremgangsmåde ifølge krav 10, hvor overfladen er en nanopartikel.
12. Fremgangsmåde ifølge krav 11, hvor nanopartiklen er metallisk.
13. Fremgangsmåde ifølge krav 12, hvor nanopartiklen er en guldnanopartikel, en sølvnanopartikel, en platinnanopartikel, en aluminiumnanopartikel, en palladiumnanopartikel, en kobbernanopartikel, en koboltnanopartikel, en indiumnanopartikel og en nikkelnanopartikel.
14. Fremgangsmåde ifølge et hvilket som helst af kravene 10- 13, hvor overfladen fjernes mindst delvist efter krydsbindingen.
15. Fremgangsmåde ifølge et hvilket som helst af kravene 10- 14, hvor det første biomolekyle og det andet biomolekyle er valgt fra gruppen, der består af et polynukleotid, et peptid, et polypeptid, et phospholipid, et oligosaccharid, et lille molekyle, et terapeutisk middel, et kontrastmiddel og kombinationer deraf.
16. Fremgangsmåde ifølge et hvilket som helst af kravene 10-15, hvor det første biomolekyle og det andet biomolekyle omfatter mindst én alkyndel, hvor alkyndelen aktiveres ved kontakt med overfladen, hvor aktiveringen gør alkynen modtagelig for et nukleofil, og hvor aktiveringen forårsager krydsbinding af det første biomolekyle til det andet biomolekyle.
17. Sammensætning, der omfatter et polyvalent nanokonjugat, der omfatter en overflade, hvilket nanokonjugat yderligere omfatter en flerhed af polynukleotider, hvor en spacer-ende af hvert af polynukleotiderne i flerheden er modificeret og er krydsbundet med et kemikalie, der krydsbinder flerheden af polynukleotider, hvor overfladen eventuelt fjernes mindst delvist efter krydsbindingen.
18. Sammensætning ifølge krav 17, hvor modificeringen er valgt fra gruppen, der består af en amin, et amid, en alkohol, en ester, et aldehyd, en keton, en thiol, et disulfid, en carboxylsyre, en phenol, en imidazol, en hydrazin, en hydrazon, et azid og en alkyn.
19. Sammensætning ifølge krav 17 eller krav 18, hvor kemikaliet er valgt fra gruppen, der består af disuccinimidylglutarat, disuccinimidylsuberat, bis[sulfosuccinimidyl]suberat, tris-succinimidylaminotriacetat, succinimidyl-4-hydrazinonicotinatacetonehydrazon, succinimidyl-4-hydrazidoterephthalathydrochlorid, succinimidyl-4-formylbenzoat, dithiobis[succinimidylpropionat], 3,3'-dithiobis[sulfosuccinimidylpropionat], disuccinimidyltartarat, bis[2-(succinimidooxycarbonyloxy)ethyl]sulfon, ethylenglycol-bis[succinimidylsuccinat], ethylenglycol-bis[sulfosuccinimidylsuccinat], dimethyladipimidat»2HC1, dimethylpimelimidat»2HC1, dimethylsuberimidat»2HCl, 1,5-difluor-2,4-dinitrobenzen, β-[tris(hydroxymethyl)phosphino]propionsyre, bismaleimidoethan, 1,4-bismaleimidobutan, bismaleimidohexan, tris[2- maleimidoethyl]amin, 1,8-bismaleimidodiethylenglycol, 1,11-bismaleimidotriethylenglycol, 1,4-bismaleimidyl-2,3- dihydroxybutan, dithiobismaleimidoethan, 1,4-di-[3'-(2'- pyridyldithio)propionamido]butan, 1, 6-hexanbisvinylsulfon, bis[b-(4-azidosalicylamido)ethyl]disulfid, N-(a- maleimidoacetoxy)succinimidester, N-[β- maleimidopropyloxy]succinimidester, N-[g- maleimidobutyryloxy]succinimidester, N-[g- maleimidobutyryloxy]sulfosuccinimidester, m-maleimidobenzoyl-N-hydroxysuccinimidester, m-maleimidobenzoyl-N- hydroxysulfosuccinimidester, succinimidyl 4-[N- maleimidomethyl]cyclohexan-l-carboxylat, sulfosuccinimidyl-4-[N-maleimidomethyl]cyclohexan-l-carboxylat, N-e- maleimidocaproyloxy]succinimidester, N-e- maleimidocaproyloxy]sulfosuccinimidester, succinimidyl-4-[p-maleimidophenyl]butyrat, sulfosuccinimidyl-4-[p- maleimidophenyl]butyrat, succinimidyl-6-[β- maleimidopropionamido]hexanoat, succinimidyl-4-[N- maleimidomethyl]cyclohexan-l-carboxy-[6-amidocaproat], N-[k-maleimidoundecanoyloxy]sulfosuccinimidester, N-succinimidyl-3-(2-pyridyldithio)propionat, succinimidyl-6-(3-[2- pyridyldithio]propionamido)hexanoat, 4- succinimidyloxycarbonylmethyl-a-[2-pyridyldithio]toluen, 4- sulfosuccinimidyl-6-methyl-a-(2- pyridyldithio)toluamido]hexanoat), N-succinimidyliodacetat, succinimidyl-3-[bromacetamido]propionat, N-succinimidyl[4- iodoacetyl]aminobenzoat, N-sulfosuccinimidyl[4- iodacetyl]aminobenzoat, N-hydroxysuccinimidyl-4- azidosalicylsyre, N-5-azido-2-nitrobenzoyloxysuccinimid, N-hydroxysulfosuccinimidyl-4-azidobenzoat, sulfosuccinimidyl[4-azidosalicylamido]hexanoat, N-succinimidyl-6-(4'-azido-2'- nitrophenylamino)hexanoat, N-sulfosuccinimidyl-6- (4'-azido-2 ' -nitrophenylamino)hexanoat, sulfosuccinimidyl- (perfluorazidobenzamido)-ethyl-1,3'-dithioproprionat, sulfosuccinimidyl-2-(m-azido-o-nitrobenzamido)-ethyl-1,3'-proprionat, sulfosuccinimidyl-2-[7-amino-4-methylcoumarin-3-acetamido]ethyl-1,3'dithiopropionat, succinimidyl-4,4'- azipentanoat, succinimidyl-6-(4,4'-azipentanamido)hexanoat, succinimidyl-2-([4,4'-azipentanamido]ethyl)-1,3'-dithioproprionat, sulfosuccinimidyl-4,4'-azipentanoat, sulfosuccinimidyl-6-(4,4'-azipentanamido)hexanoat, sulfosuccinimidyl-2-([4,4'-azipentanamido]ethyl)-1,3'-dithioproprionat, dicyclohexylcarbodiimid, l-ethyl-3-[3- dimethylaminopropyl]carbodiimidhydrochlorid, N-[4-(p- azidosalicylamido)butyl]-3'-(2'-pyridyldithio)propionamid, N-[β-maleimidopropionsyre]hydrazid, trifluoreddikesyresalt, [N- e-maleimidocapronsyre]hydrazid, trifluoreddikesyresalt, 4-(4- N-maleimidophenyl)smørsyrehydrazidhydrochlorid, N-[k- maleimidoundecansyre]hydrazid, 3-(2- pyridyldithio)propionylhydrazid, p-azidobenzoylhydrazid, N-[p-maleimidophenyl]isocyanat og succinimidyl-[4-(psoralen-8-yloxy)]butyrat.
20. Sammensætning ifølge et hvilket som helst af kravene 17-19, hvor overfladen omfatter en nanopartikel.
21. Sammensætning ifølge krav 20, hvor nanopartiklen er valgt fra gruppen, der består af en kugle, en stav og en prisme.
22. Sammensætning ifølge krav 21, hvor nanopartiklen er metallisk.
23. Sammensætning ifølge krav 22, hvor nanopartiklen er en guldnanopartikel, en sølvnanopartikel, en platinnanopartikel, en aluminiumnanopartikel, en palladiumnanopartikel, en kobbernanopartikel, en koboltnanopartikel, en indiumnanopartikel og en nikkelnanopartikel.
DK10827627.0T 2009-10-30 2010-11-01 Skabelonstyrede nanokonjugater DK2494075T3 (da)

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US25664009P 2009-10-30 2009-10-30
US37455010P 2010-08-17 2010-08-17
US38684610P 2010-09-27 2010-09-27
PCT/US2010/055018 WO2011053940A2 (en) 2009-10-30 2010-11-01 Templated nanoconjugates

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EP (1) EP2494075B1 (da)
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