DE602005017362D1 - Stabilisierte rnas als transfektionskontrollen und silencing-reagentien - Google Patents

Stabilisierte rnas als transfektionskontrollen und silencing-reagentien

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Publication number
DE602005017362D1
DE602005017362D1 DE602005017362T DE602005017362T DE602005017362D1 DE 602005017362 D1 DE602005017362 D1 DE 602005017362D1 DE 602005017362 T DE602005017362 T DE 602005017362T DE 602005017362 T DE602005017362 T DE 602005017362T DE 602005017362 D1 DE602005017362 D1 DE 602005017362D1
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DE
Germany
Prior art keywords
nucleotide
terminal
sense
antisense
alkyl
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
DE602005017362T
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English (en)
Inventor
Devin Leake
Anastasia Khvorova
Yuriy Fedorov
Michael O Delaney
Barbara Robertson
Emily Anderson
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Dharmacon Inc
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Dharmacon Inc
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Application filed by Dharmacon Inc filed Critical Dharmacon Inc
Publication of DE602005017362D1 publication Critical patent/DE602005017362D1/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/321—2'-O-R Modification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/322—2'-R Modification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/50—Methods for regulating/modulating their activity
    • C12N2320/51—Methods for regulating/modulating their activity modulating the chemical stability, e.g. nuclease-resistance
DE602005017362T 2004-02-06 2005-02-04 Stabilisierte rnas als transfektionskontrollen und silencing-reagentien Active DE602005017362D1 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US54264604P 2004-02-06 2004-02-06
US54364004P 2004-02-10 2004-02-10
US57227004P 2004-05-18 2004-05-18
PCT/US2005/003365 WO2005078094A2 (en) 2004-02-06 2005-02-04 Stabilized rnas as transfection controls and silencing reagents

Publications (1)

Publication Number Publication Date
DE602005017362D1 true DE602005017362D1 (de) 2009-12-10

Family

ID=34865155

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602005017362T Active DE602005017362D1 (de) 2004-02-06 2005-02-04 Stabilisierte rnas als transfektionskontrollen und silencing-reagentien

Country Status (5)

Country Link
US (1) US20070269889A1 (de)
EP (2) EP2123759B1 (de)
AT (1) ATE447024T1 (de)
DE (1) DE602005017362D1 (de)
WO (1) WO2005078094A2 (de)

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JP2022518765A (ja) 2019-01-24 2022-03-16 ノースウエスタン ユニバーシティ 心房細動の遺伝子療法による治療

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US20070269889A1 (en) 2007-11-22
ATE447024T1 (de) 2009-11-15
EP2123759B1 (de) 2014-01-15
WO2005078094A3 (en) 2006-03-23
EP2123759A3 (de) 2011-03-09
WO2005078094A2 (en) 2005-08-25
EP1718747A2 (de) 2006-11-08
EP2123759A2 (de) 2009-11-25
EP1718747B1 (de) 2009-10-28

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