JPH0438394B2 - - Google Patents

Info

Publication number
JPH0438394B2
JPH0438394B2 JP63212641A JP21264188A JPH0438394B2 JP H0438394 B2 JPH0438394 B2 JP H0438394B2 JP 63212641 A JP63212641 A JP 63212641A JP 21264188 A JP21264188 A JP 21264188A JP H0438394 B2 JPH0438394 B2 JP H0438394B2
Authority
JP
Japan
Prior art keywords
lipase
recombinant
promoter
humicola
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
JP63212641A
Other languages
English (en)
Japanese (ja)
Other versions
JPH01157383A (ja
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DK450087A external-priority patent/DK450087D0/da
Priority claimed from DK656087A external-priority patent/DK656087D0/da
Priority claimed from DK205488A external-priority patent/DK205488D0/da
Application filed filed Critical
Publication of JPH01157383A publication Critical patent/JPH01157383A/ja
Publication of JPH0438394B2 publication Critical patent/JPH0438394B2/ja
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP63212641A 1987-08-28 1988-08-29 組換え体ヒュミコーラリパーゼおよびその調製方法 Granted JPH01157383A (ja)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
DK450087A DK450087D0 (da) 1987-08-28 1987-08-28 Fremgangsmaade til fremstilling af proteiner
DK4500/87 1987-08-28
DK656087A DK656087D0 (da) 1987-12-15 1987-12-15 Enzym
DK6560/87 1987-12-15
DK2054/88 1988-04-15
DK205488A DK205488D0 (da) 1988-04-15 1988-04-15 Enzym

Publications (2)

Publication Number Publication Date
JPH01157383A JPH01157383A (ja) 1989-06-20
JPH0438394B2 true JPH0438394B2 (OSRAM) 1992-06-24

Family

ID=27221490

Family Applications (1)

Application Number Title Priority Date Filing Date
JP63212641A Granted JPH01157383A (ja) 1987-08-28 1988-08-29 組換え体ヒュミコーラリパーゼおよびその調製方法

Country Status (5)

Country Link
EP (1) EP0305216B1 (OSRAM)
JP (1) JPH01157383A (OSRAM)
AT (1) ATE125865T1 (OSRAM)
DE (1) DE3854249T2 (OSRAM)
ES (1) ES2076939T3 (OSRAM)

Families Citing this family (831)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5278066A (en) * 1985-08-09 1994-01-11 Gist-Brocades Nv Molecular cloning and expression of gene encoding lipolytic enzyme
US5766912A (en) * 1986-03-17 1998-06-16 Novo Nordisk A/S Humicola lipase produced in aspergillus
US5536661A (en) * 1987-03-10 1996-07-16 Novo Nordisk A/S Process for the production of protein products in aspergillus
US5830735A (en) * 1987-03-06 1998-11-03 Gist-Brocades Nv Method for producing lipolytic enzymes using transformed Pseudomonas
GB8810954D0 (en) * 1988-05-09 1988-06-15 Unilever Plc Enzymatic detergent & bleaching composition
US5292448A (en) * 1988-05-10 1994-03-08 Lever Brothers Company, Division Of Conopco, Inc. Enzymatic detergent composition
GB8915658D0 (en) * 1989-07-07 1989-08-23 Unilever Plc Enzymes,their production and use
US5658871A (en) * 1989-07-07 1997-08-19 Lever Brothers Company, Division Of Conopco, Inc. Microbial lipase muteins and detergent compositions comprising same
DK173590D0 (da) * 1990-06-06 1990-07-19 Novo Nordisk As Rekombinante terapeutiske lipaser
US5869438A (en) * 1990-09-13 1999-02-09 Novo Nordisk A/S Lipase variants
US5892013A (en) * 1990-09-13 1999-04-06 Novo Nordisk A/S Lipase variants
EP0548228B1 (en) * 1990-09-13 1998-08-12 Novo Nordisk A/S Lipase variants
DK46693D0 (OSRAM) * 1993-04-23 1993-04-23 Novo Nordisk As
ATE176498T1 (de) * 1991-04-02 1999-02-15 Novo Nordisk As Xylanase, entsprechende rekombinante dna-sequenz, xylanase enthaltendes agens, und seine verwendung
DK0585285T3 (da) 1991-05-01 1999-05-10 Novo Nordisk As Stabiliserede enzymer
CA2124316A1 (en) * 1991-11-26 1993-06-10 Allan Svendsen Protease-stable proteins
DK88892D0 (da) * 1992-07-06 1992-07-06 Novo Nordisk As Forbindelse
DK104592D0 (da) * 1992-08-21 1992-08-21 Novo Nordisk As Fremgangsmaade
DK154292D0 (da) * 1992-12-23 1992-12-23 Novo Nordisk As Nyt enzym
DK39693D0 (da) † 1993-04-02 1993-04-02 Novo Nordisk As Enzym
DK47493D0 (da) * 1993-04-27 1993-04-27 Novo Nordisk As Fremgangsmaade til fremstilling af ost
DE4329463A1 (de) * 1993-09-01 1995-03-02 Cognis Bio Umwelt Mehrenzymgranulate
AU690617B2 (en) * 1993-12-01 1998-04-30 Novo Nordisk Biotech, Inc. (Aspergillus foetidus) expression system
CA2178007A1 (en) * 1993-12-01 1995-06-08 Randy M. Berka Aspergillus expression system
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US5714373A (en) * 1995-02-27 1998-02-03 Recombinant Biocatalysis, Inc. Thermococcus AV4 and enzymes produced by the same
DE19515072A1 (de) * 1995-04-28 1996-10-31 Cognis Bio Umwelt Cellulasehaltiges Waschmittel
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MY129458A (en) 1995-06-07 2007-04-30 Danisco A method of improving the properties of a flour dough, a flour dough improving composition and improved food products
US6936289B2 (en) 1995-06-07 2005-08-30 Danisco A/S Method of improving the properties of a flour dough, a flour dough improving composition and improved food products
WO1997007202A1 (en) * 1995-08-11 1997-02-27 Novo Nordisk A/S Novel lipolytic enzymes
BE1009650A5 (fr) * 1995-10-12 1997-06-03 Genencor Int Systeme d'expression, vecteur et cellule transformee par ce vecteur.
JP3792725B2 (ja) * 1996-01-19 2006-07-05 ノボザイムス，インコーポレイティド 糸状菌の形態変異体
ATE373493T1 (de) 1996-07-05 2007-10-15 Novozymes As Alpha-amylase transkriptionsfaktor
CN1136311C (zh) 1996-11-04 2004-01-28 诺沃奇梅兹有限公司 枯草杆菌酶变异体和组合物
JP4044143B2 (ja) 1996-11-04 2008-02-06 ノボザイムス アクティーゼルスカブ ズブチラーゼ変異体及び組成物
DE69835111T2 (de) 1997-04-09 2007-01-04 Danisco A/S Verwendung von Lipase zur Verbesserung von Teigen und Backwaren
DK1525798T3 (da) 1997-12-22 2012-03-05 Novozymes As Carbohydrat-oxidase og anvendelse deraf i bagning
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US6156552A (en) * 1998-02-18 2000-12-05 Novo Nordisk A/S Lipase variants
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CN100482801C (zh) 1999-03-22 2009-04-29 诺沃奇梅兹有限公司 用于在真菌细胞中表达基因的启动子
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DE60040285D1 (de) 1999-05-20 2008-10-30 Novozymes As Subtilase enzyme der i-s1 und i-s2 untergruppen mit mindestens einer zusätzlichen aminosäure zwischen position 127 und 128
AU4392800A (en) 1999-05-20 2000-12-12 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additionalamino acid residue between positions 126 and 127
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AU4392700A (en) 1999-05-20 2000-12-12 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additionalamino acid residue between positions 129 and 130
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ATE125865T1 (de) 1995-08-15
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