MX339581B - Variantes de subtilasa. - Google Patents

Variantes de subtilasa.

Info

Publication number
MX339581B
MX339581B MX2012003386A MX2012003386A MX339581B MX 339581 B MX339581 B MX 339581B MX 2012003386 A MX2012003386 A MX 2012003386A MX 2012003386 A MX2012003386 A MX 2012003386A MX 339581 B MX339581 B MX 339581B
Authority
MX
Mexico
Prior art keywords
subtilase variants
relates
present
protease variants
variant
Prior art date
Application number
MX2012003386A
Other languages
English (en)
Other versions
MX2012003386A (es
Inventor
Beier Lars
Carsten Franz Knötzel Jürgen
Benie Astrid
Norman Hockauf Maria
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Publication of MX2012003386A publication Critical patent/MX2012003386A/es
Publication of MX339581B publication Critical patent/MX339581B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Detergent Compositions (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La presente invención se refiere a una variante de subtilisina 309 con SEQ ID NO. 1, caracterizada porque comprende las sustituciones 9R, 15T, 68 A, 218D y 245R, en donde la variante comprende además por lo menos una de las siguientes modificaciones: 61{D,E}, 62 {D,E}, 76 {D,E}, *97aG, 98{G, S}, 99G, 101G,120{V, Q,D}, 131{T,S},137H, 194P, 228V, 230V,261D, en donde las variantes tienen capacidades mejoradas de remoción de huevo tal como la remoción mejorada de yema de huevo hervida de superficies y en donde las posiciones corresponden a las posiciones del polipéptido maduro de SEQ ID NO:2 [BPN´].
MX2012003386A 2009-09-25 2010-09-24 Variantes de subtilasa. MX339581B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP09171308 2009-09-25
PCT/EP2010/064171 WO2011036263A1 (en) 2009-09-25 2010-09-24 Subtilase variants

Publications (2)

Publication Number Publication Date
MX2012003386A MX2012003386A (es) 2012-06-27
MX339581B true MX339581B (es) 2016-06-01

Family

ID=43033187

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2012003386A MX339581B (es) 2009-09-25 2010-09-24 Variantes de subtilasa.

Country Status (10)

Country Link
US (1) US20120172280A1 (es)
EP (1) EP2480650B1 (es)
JP (2) JP5947213B2 (es)
CN (1) CN102648273B (es)
AU (1) AU2010299799B2 (es)
BR (1) BR112012006487A8 (es)
CA (1) CA2775045A1 (es)
MX (1) MX339581B (es)
RU (1) RU2651525C2 (es)
WO (1) WO2011036263A1 (es)

Families Citing this family (291)

* Cited by examiner, † Cited by third party
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BR112012006487A2 (pt) 2016-11-22
JP2013505711A (ja) 2013-02-21
AU2010299799B2 (en) 2015-10-29
EP2480650B1 (en) 2017-03-22
CN102648273B (zh) 2017-04-26
CA2775045A1 (en) 2011-03-31
WO2011036263A1 (en) 2011-03-31
JP5947213B2 (ja) 2016-07-06
MX2012003386A (es) 2012-06-27
JP2016052334A (ja) 2016-04-14
AU2010299799A1 (en) 2012-04-05
EP2480650A1 (en) 2012-08-01
CN102648273A (zh) 2012-08-22
US20120172280A1 (en) 2012-07-05
BR112012006487A8 (pt) 2018-04-24

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