JP2009510531A - 1以上の生物学的構造体のマルチモダリティ顕微鏡画像生成を実行する装置及び方法 - Google Patents
1以上の生物学的構造体のマルチモダリティ顕微鏡画像生成を実行する装置及び方法 Download PDFInfo
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Abstract
【選択図】図10
Description
(関連出願の相互参照)
を用いることができる。代表的な画像は、X−Y走査機構835の位置毎にデータを取得して生成することができる。サンプルからの蛍光は、二色性ミラー又はフィルタ853及び第二検出器865を用いて更に検出することができる。
(実施例)
代表的な方法
卓上型典型OCTシステムとOFDIシステム
代表的FFOCMシステム
代表的なSECMシステム
検体作成、エタノール処理および組織学
代表的な結果
生体OFDI技法を用いた胎児心臓の四次元画像化
生体胎児心臓に対する高分解能OFDI手法
体外FFOCM手法を用いた胎児心臓の高分解能三次元画像の生成
生体SECM手法による胎児心臓の高速画像生成
アフリカツメガエル幼生の動脈瘤壁拡張
エタノール暴露による心異常
代表的結果の考察
of mesenchymal stem cell in myocardial infarction”(心筋梗塞に対する間充織幹細胞の 生体磁気共鳴画像の生成)、Circulation 107、 pp.2290−2293)、)、及びF.Wiesmann et al.、“Developmental changes of cardiac function and mass assessed with MRI innenotal、juvenile、and adult mice”(MRIに関わる新生、若年性および成体ハツカネズミの心機能および主要部位の発育上の変化)、AmJ Physiol Heart Circ Physiol 278、pp.H652−657(2000)参照)、マイクロCT(M.Malyar et al.、“Relationship between arterial diameter and perfused 組織 volume in myocardial microcirculation: a micro―CT−based analysis(心筋微小循環における動脈径と分散組織の体積との相関:マイクロCTを用いた分析)”、Am J Physiol heart Circ Physiol 286、pp。H2386−2392(2004)、and C.T.Badea et al.“4−D micro―CT of the mouse heart”(マウス心臓の4−DマイクローCT)、Mo I Imaging 4、pp.130−135(2005)、超音波(S.Srinivasan et al., “Noninvasive, in utero imaging of mouse embryonic heart development with 40−MHz echocardiography”, Circulation 98, pp.912−918 (1998)参照)及びPET(L.W.Dobrucki et al., “Molecular cardiovascular imaging”, Curr Cardiol Rep 7, pp.130−135 (2005), and L.Stegger et al., “Monitoring left ventricular dilation in mice with PET,”(PETを用いたマウスの左心室肥大のモニタリング)JNucl Med 46, pp.1516− 1521 (2005)を参照)を用いて実証してきた。
表1.胎児心臓の光学的画像生成を目的とした内因性コントラストモダリティの比較
灰色の陰影を付けた細胞は、最大の横方向分解能特性、軸方向分解能特性及びフレームレート特性を持つ画像生成技術を表す。
Claims (31)
- 第1のモダリティに基づいて少なくとも一つのサンプルの少なくとも一つの部位から受信した第1の信号に関連する第1のデータと、前記第1のモダリティとは異なる第2のモダリティに基づいて前記少なくとも一つのサンプルから受信した第2の信号に関連する第2のデータとを送出し、更に、基準に関連する第3のデータを受信するように構成された少なくとも一つの第1の機器と、
前記第1のデータ、前記第2のデータ及び前記第3のデータに基づいて更なるデータを生成するように構成された、少なくとも一つの第2の機器を備える、装置。 - 前記第1のモダリティがスペクトル符号化共焦点顕微鏡技術である、請求項1記載の装置。
- 前記第2のモダリティが、蛍光画像生成技術である、請求項1記載の装置。
- 前記少なくとも一つの第1の機器及び前記少なくとも一つの第2の機器と関連付けられた顕微鏡装置を更に備える、請求項1記載の装置。
- 電磁放射を前記少なくとも一つの部位に照射するように構成されたビーム走査機構を備える、請求項1記載の装置。
- 前記少なくとも一つの第2の機器は、(i)二次元画像又は(ii)三次元画像の少なくとも一つを前記更なるデータの関数として生成する、請求項1記載の装置。
- 前記第1のデータ及び前記第2のデータは、ほぼ同時に取得される、請求項1記載の装置。
- 前記第1のデータ及び前記第2のデータは、前記少なくとも一つのサンプルの略同一の位置に関連付けられる、請求項1記載の装置。
- 前記第1のデータ及び前記第2のデータの少なくとも一つは、前記第1のデータ及び前記第2のデータの他方に基づいて取得する、請求項1記載の装置。
- 前記少なくとも一つの第1の機器及び前記少なくとも一つの第2の機器は、一つのプローブ又は単一の筐体に設けられる、請求項1記載の装置。
- 前記少なくとも一つの第1の機器及び前記少なくとも一つの第2の機器は、共通の部品を有する、請求項1記載の装置。
- 前記共通の部品は、光学装置又は検出器装置の少なくとも一つに存在する、請求項11記載の装置。
- 前記少なくとも一つの第1の機器は、スペクトル符号化顕微鏡検査情報を取得するように構成された、請求項1記載の装置。
- 前記少なくとも一つの第1の機器は、明視野、暗視野、位相コントラスト、偏光、上部反射性(epirefiectance)又は反射顕微鏡検査情報の少なくとも一つを取得するように構成された、請求項1記載の装置。
- 前記第1のモダリティを前記第2のモダリティに変更するように構成された更なる装置を備える、請求項1記載の装置。
- 前記少なくとも一つの第1の機器は、複数の波長を有する光源装置から提供される信号に関連する光コヒーレンストモグラフィー情報を取得するように構成され、
前記第2の信号と第3の信号の間のスペクトル干渉を前記複数の波長の関数として検出するように構成された複数の検出器を更に備える、請求項1記載の装置。 - 前記少なくとも一つの第1の機器は、波長が時間とともに変化する光源装置から提供される信号に関連する光コヒーレンストモグラフィー情報を取得するように構成された、請求項1記載の装置。
- 前記少なくとも一つの機器は、基準に関連する第3のデータを受信するように構成され、前記少なくとも一つの第2の機器は更に、前記第3のデータを生成するように構成された、請求項1記載の装置。
- 前記第1のデータ及び前記第2のデータの少なくとも一つに基づいて前記少なくとも一つの第1の機器を制御するように構成された少なくとも一つの第3の機器を備える、請求項1記載の装置。
- 前記第1のデータ及び前記第2のデータに基づいて画像を生成するように構成された少なくとも一つの第4の機器を備える、請求項1記載の装置。
- 前記第1のデータに基づいて少なくとも一つの第1の画像を、前記第2のデータに基づいて少なくとも一つの第2の画像を生成するように構成された少なくとも一つの第5の機器を備え、前記第1の画像と前記第2の画像は、前記第1のデータと前記第2のデータの関数としてお互いに関連付けられている、請求項1記載の装置。
- 前記少なくとも一つの第1の機器は、光コヒーレンストモグラフィー情報を取得するように構成された、請求項1記載の装置。
- 前記少なくとも一つの第1の機器は、光周波数領域干渉分光情報を取得するように構成された、請求項1記載の装置。
- 第1のモダリティに基づいて少なくとも一つのサンプルの少なくとも一つの部位から受信した第1の信号に関連する第1のデータと、前記第1のモダリティとは異なる第2のモダリティに基づいて前記少なくとも一つのサンプルから受信した第2の信号に関連する第2のデータと、各々が前記第1のモダリティ及び第3のモダリティとは異なる更なるモダリティに基づき、かつ、前記少なくとも一つのサンプルから受信した第2の信号に関連する、少なくとも一つの第3のデータとを送出するように構成された、少なくとも一つの第1の機器と、
前記第1のデータ、前記第2のデータ及び前記第3のデータに基づき更なるデータを生成するように構成された、少なくとも一つの第2の機器を備える、装置。 - 前記少なくとも一つの第1の機器が、光コヒーレンストモグラフィー情報を取得するように構成された、請求項24記載の装置。
- 前記少なくとも一つの第1の機器が、光コヒーレンス顕微鏡検査情報を取得するように構成された、請求項24記載の装置。
- 前記少なくとも一つの第1の機器が、全域(full field)光コヒーレンス顕微鏡検査情報を取得するように構成された、請求項24記載の装置。
- 第1のスペクトル符号化モダリティに基づいて少なくとも一つのサンプルの少なくとも一つの部位から受信した第1の信号に関連する第1のデータと、第2の非スペクトル符号化モダリティに基づいて前記少なくとも一つのサンプルから受信した第2の信号に関連する第2のデータとを送出するように構成された少なくとも一つの第1の機器と、
前記第1のデータと前記第2のデータに基づいて更なるデータを生成するように構成された少なくとも一つの第2の機器を備える、装置。 - 第1のモダリティに基づいて少なくとも一つのサンプルの少なくとも一つの部位から受信した第1の信号に関連する第1のデータと、前記第1のモダリティとは異なる第2のモダリティに基づいて前記少なくとも一つのサンプルから受信した第2の信号に関連する第2のデータと、を送出するステップと、
基準に関連する第3のデータを受信するステップと、
前記第1のデータ、前記第2のデータ及び前記第3のデータに基づいて更なるデータを生成するステップと、を備える方法。 - 第1のモダリティに基づいて少なくとも一つのサンプルの少なくとも一つの部位から受信した第1の信号に関連する第1のデータと、前記第1のモダリティとは異なる第2のモダリティに基づいて前記少なくとも一つのサンプルから受信した第2の信号に関連する第2のデータと、各々が、前記第1のモダリティ及び前記第2のモダリティとは異なる更なるモダリティに基づき、前記少なくとも一つのサンプルから受信した第2の信号に関連する、少なくとも一つの第3のデータと、を送出するステップと、
前記第1のデータ、前記第2のデータ及び前記少なくとも一つの第3のデータに基づいて更なるデータを生成するステップと、を備える方法。 - 第1のスペクトル符号化モダリティに基づいて少なくとも一つのサンプルの少なくとも一つの部位から受信した第1の信号に関連する第1のデータと、第2の非スペクトル符号化モダリティに基づいて前記少なくとも一つのサンプルから受信した第2の信号に関連する第2のデータと、を送出するステップと、
前記第1のデータと前記第2のデータに基づいて更なるデータを生成するステップと、を備える方法。
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