EP0331376B1 - Rekombinante DNA, sie enthaltendes Bakterium der Gattung Pseudomonas und ihre Verwendung zur Herstellung von Lipase - Google Patents
Rekombinante DNA, sie enthaltendes Bakterium der Gattung Pseudomonas und ihre Verwendung zur Herstellung von Lipase Download PDFInfo
- Publication number
- EP0331376B1 EP0331376B1 EP89301872A EP89301872A EP0331376B1 EP 0331376 B1 EP0331376 B1 EP 0331376B1 EP 89301872 A EP89301872 A EP 89301872A EP 89301872 A EP89301872 A EP 89301872A EP 0331376 B1 EP0331376 B1 EP 0331376B1
- Authority
- EP
- European Patent Office
- Prior art keywords
- lipase
- bacterium
- dna
- recombinant dna
- pseudomonas
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
- 239000001052 yellow pigment Substances 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
- C12N9/20—Triglyceride splitting, e.g. by means of lipase
Definitions
- the present invention relates to a recombinant DNA, a bacterium of the genus Pseudomonas containing it, and a process for preparing lipase by using it.
- Lipase is an enzyme capable of hydrolyzing a lipid, and utilized in an extensive field such as application in fats and oils processing, diagnostic reagents, detergents, etc.
- the present inventors considered that it may be possible to accumulate lipase in a large quantity in the culture medium if DNA fractions containing lipase genes, and plasmids are used, and also transformants comprised of bacteria of the genus Pseudomonas as a host are obtained, and they made intensive studies to accomplish the present invention.
- the present invention provides a recombinant DNA, a bacterium of the genus Pseudomonas containing it, and a process for preparing lipase by using it, which process comprises separating a DNA fraction originating from the bacterium of the genus Pseudomonas and containing a lipase gene to obtain plasmids having said DNA fraction, inserting DNA into the bacterium of the genus Pseudomonas by use of these plasmids to obtain a transformant, culturing said transformant to accumulate lipase outside the bacterial body in a large quantity, and collecting the accumulated lipase.
- a novel recombinant DNA capable of being replicated in a bacterium of the genus Pseudomonas, and in which a DNA containing a gene that codes for lipase has been inserted into a wide host range plasmid vector.
- a novel bacterium of the genus Pseudomonas inserted with a novel recombinant DNA in which a DNA that originates from a bacterium of the genus Pseudomonas and carries genetic information of the lipase has been inserted into a vector.
- a novel DNA in which a DNA that originates from a bacterium of the genus Pseudomonas and carries genetic information of the lipase comprises a structural gene of the lipase and a gene participating in the production of the lipase.
- a novel DNA in which a structural gene of the lipase comprises the amino acid sequence shown in Fig. 3A.
- Fig. 1 shows as an example a restriction map prepared by separating by various restriction enzymes a plasmid pLiPl having the DNA arrangement that codes for lipase of Pseudomonas cepacia M-12-33 (FERM BP-2293) and on the basis of the measurement by use of an agarose gel electrophorasis.
- Fig. 2 shows a result of examination on whether or not a halo is formed in a triolein culture medium in respect of a product resulted from transformation of a Pseudomonas cepacia HW10 strain by subcloning to pFL100, of each DNA fraction obtained by cleavage of said plasmid pLiPl with various restriction enzymes.
- Figs. 3A and 3B each show the amino acid sequence of the DNA that carries the genetic information of the lipase of Pseudomonas cepacia, in which Fig. 3A shows an amino acid sequence of lipase and Fig. 3B shows an amino acid sequence of the DNA that participates in the occurrence of lipase although extensive errors have been identified in this figure.
- the underlined portion in Fig. 3A indicates the region at which the N-terminal amino acid sequence of a purified lipase protein has been determined.
- a source of the chromosomal DNA containing the lipase gene of the present invention is supplied from bacteria of the genus Pseudomonas having the ability of producing lipase.
- bacteria of the genus Pseudomonas having the ability of producing lipase includes the strain named Pseudomonas sp. M-12-33 isolated by Arima et al [Agr. Biol. Chem., 30 , 515 (1966)] .
- This strain has the following bacteriological properties.
- the chromosomal DNA and transformant according to the present invention can be prepared by the following procedures.
- the Pseudomonas cepacia M-12-33 strain ( FERM BP-2293 ) is cultured overnight at 30°C under aerobic conditions, using an LB medium (tryptone: 1.0 %; yeast extract: 0.5 %; sodium chloride: 1.0 %). Bacterial bodies are collected, followed by extraction and purification of the chromosomal DNA by utilizing a known method such as the Saito and Miura's method [Biochem. Biophys. Acta., 72 , 619-629 (1963)] .
- Plasmid vectors may preferably be vectors capable of being replicated in a host, having a known restriction enzyme incision portion, and having a selective marker for chemical resistance and the like.
- they include wide host range plasmid vectors such as RSF1010, R1b679 and R1162. More specifically, there can be used pFL100 obtained by incorporating a kanamycin-resistant gene into RSF1010.
- the vector DNA thus obtained is cleaved with a restriction enzyme such as EcoRI, to which the chromosomal DNA is cleaved with the like restriction enzyme and purified is joined according to a known method using ligase or the like, thus obtaining a recombinant plasmid.
- a restriction enzyme such as EcoRI
- bacteria of the genus Pseudomonas as exemplified by Pseudomonas cepacia, Pseudomonas putida, etc. are subjected to transformation.
- the transformation is carried out by utilizing a calcium chloride method, a rubidium chloride method, an electroporation method.
- Transformed bacterium having the ability of producing lipase may be selectively separated with simplicity by using an agar medium containing triglycerides such as tributyrin and triolein emulsified with polyvinyl alcohol, and having an antibiotic substance in a predetermined concentration.
- triglycerides such as tributyrin and triolein emulsified with polyvinyl alcohol
- the bacteria containing lipase genes can be selectively separated by separating from among the transformed bacteria a strain that forms a large clearing zone in the agar medium.
- a plasmid DNA containing the lipase gene is obtained by using a known method such as an alkali method or a boiling method.
- the lipase is further produced by using the bacteria of the genus Pseudomonas obtained by insertion of the thus obtained plasmid DNA according to the present invention.
- the Pseudomonas cepacia M-12-33 strain having the novel recombinant plasmid is cultured by using a known medium, and the lipase can be obtained by separating it from the culture supernatant and purifying the separated one.
- a substrate solution emulsified by mixing 75 ml of olibe oil, in 225 ml of 2 % polyvinyl alcohol (PVA) solution, and 4 ml of a 0.2 M Mcllvaine's buffer solution (pH 7) are charge and the mixture is pre-warmed at 37°C for 5 minutes.
- PVA polyvinyl alcohol
- pH 7 a 0.2 M Mcllvaine's buffer solution
- Pseudomonas cepacia M-12-33 ( FERM BP-2293) was cultured overnight at 30°C under aerobic conditions with shaking by use of the LB medium, and, after collection of bacteria, the chromosomal DNA was extracted and purified according to a DNA extraction method by the Saito and Miura's method, thus obtaining 8.4 mg of the chromosomal DNA.
- a wide host range plasmid vector RSF1010 (330 ng) was cleaved with a restriction enzyme PstI to extract an 8.1 Kb fraction.
- a 1.4 Kb fraction containing a kanamycin resistant gene was prepared from 480 ng of pUC-4K (available from Pharmacia Fine Chemicals, Inc.) according to the similar operation. Both were joined with a T4 DNA ligase, thereby obtaining a plasmid vector pFL100.
- the pFL100 was purified from an Escherichia coil C600 strain according to Maniatis et al's method [Maniatis et al. Molecular Cloning, a laboratory manual, 92 - 94 (1981) ].
- the above chromosomal DNA (8.4 ⁇ g) was taken up, and, with addition of a restriction enzyme EcoRI, reacted at 37 °C for 15 minutes, to cleave the DNA in part.
- EcoRI was added to 2.5 ⁇ g of the plasmid pFL100 to carry out the reaction at 37 °C for 2 hours, thus completely cleaving the DNA.
- alkaline phosphatase was added to effect dephosphorylation.
- the cleaved chromosomal DNA and the vector plasmid DNA were mixed, and reacted with addition of DNA ligase to carry out the reaction to join DNA fractions.
- a halo-formation defective host is advantageous. Accordingly, the Pseudomonas cepacia M-12-33 strain ( FERM P No. 9871 ) was treated with nitrosoguanidine, and a variant strain HW10 that forms no halo was obtained using Antibiotic Medium 3 (available from Difco Co.) containing 0.2 % of triolein.
- the present inventors found that the electroporation method is very effective for the transformation of said strain. More specifically, said strain was cultured at 30°C using 20 ml of the LB medium until it entered the logarithmic growth phase (OD 660 0.4), and then cooled, followed by centrifugal separation at 3,500 rpm for 5 minutes. The bacterial bodies were washed with 10 ml of a buffer solution (pH 7.4) comprised of 272 mM sucrose and 7 mM sodium phosphate. Centrifugal separation was again carried out, followed by suspension in 0.8 ml of the like buffer solution.
- a buffer solution pH 7.4
- the recombinant DNA thus joined was subjected to the electroporation method to effect the transformation of the HW10 strain, and then the transformants were spreaded onto a selective medium containing 500 ⁇ g/ml of kanamycin and 0.2 % of triolein. As a result, 8 halo-forming strains were obtained.
- Plasmids were extracted from the above 8 halo-forming strains, and cleaved with the restriction enzyme EcoRI.
- the inserted DNA fractions were analyzed by agarose electrophoresis to find that the strains were divided into those into which about 10 Kb of the EcoRI fraction was inserted and those containing 6.0 Kb and 2.7 Kb of EcoRI fractions.
- An experiment using an antilipase antiserum revealed that the desired lipase gene was present on the 10 Kb EcoRI fraction. It is considered that in fact the halo-formation defective strain HW10 did not undergo the variation on the lipase gene, but turned to the halo-formation defective strain because of the variation of other genes.
- the plasmid vector containing 10 Kb of the EcoRI fraction was named pLiPl, and its restriction enzyme cleavage map is shown in Fig. 1.
- the pLiPl plasmid was cleaved with various restriction enzymes, where each DNA fraction was subcloned to pFL100 to effect transformation of the Pseudomonas cepacia HW10 strain, and whether or not the halo were formed in the triolein medium was examined in the same manner as the above. Results obtained are shown in Fig. 2.
- the plasmid pLip10 obtained by the subcloning of a 3 Kb ClaI-EcoRI fraction also produced lipase. However, no production of lipase was observed as to the plasmid containing a 2 Kb ClaI-KpnI fraction.
- the size of the main body of lipase gene presumable from the fact that the lipase has a molecular weight of 34,000, is considered to be about 1.1 Kb, but the result of the subcloning shows that 2 Kb or more of the DNA fraction is required for the production of the lipase. Then, the whole base sequence of the 3 Kb ClaI-EcoRI fraction was determined according to the Mizusawa et al's method [Nucleic Acids Res., 1 4(3), 1319-1324 (1986)].
- the 3 Kb ClaI-EcoRI fraction comprises a structural gene of lipase and a gene of the protein essential to the production of lipase.
- the amino acid sequence of these genes is shown in Figs. 3A and 3B, although figure 3B contains extensive errors.
- the Pseudomonas cepacia M-12-33 was transformed with the plasmid pLiP10 containing the above lipase gene.
- the present strain was cultured with shaking at 30°C for 3 days, using a liquid medium comprising 2.0 % of soybean oil, 0.5 % of peptone (available from Difco Co.), 0.3 % of meat extract (available from Difco Co.), 0.1 % of KH 2 PO 4 , 0.02 % of MgSO 4 ⁇ 7H 2 O, 0.001 % of FeSO 4 ⁇ 7H 2 O, and one drop of Adecanol (available from Asahi Denka).
- a culture supernatant with a lipase activity of 600 U/ml was obtained by centrifugal separation. This productivity was about 40 times the lipase productivity of the host bacterium Pseudomonas capacia M-12-33 strain.
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Claims (10)
- Isoliertes DNA-Fragment, umfassend ein Strukturgen, das für eine Lipase kodiert und ein Gen, das für ein Protein kodiert, das an der Herstellung der Lipase mitwirkt.
- Isoliertes DNA-Fragment nach Anspruch 1, das von einem Bakterium der Gattung Pseudomonas abstammt.
- Isoliertes DNA-Fragment nach Anspruch 2, wobei das Strukturgen die in Figur 3A dargestellte Aminosäuresequenz kodiert.
- Rekombinante DNA, die fähig ist, in einem Bakterium der Gattung Pseudomonas repliziert zu werden, umfassend einen Plasmidvektor mit einem breiten Wirtsspektrum und ein DNA-Fragment nach einem oder mehreren der Ansprüche 1 bis 3.
- Rekombinante DNA nach Anspruch 4, worin der Plasmidvektor RSF1010 oder ein davon abgeleitetes Plasmid ist.
- Rekombinante DNA nach Anspruch 5, worin der Plasmidvektor pFL100 ist, erhalten durch Inkorporation eines Kanamycinresistenten Gens in den Plasmidvektor RSF1010.
- Bakterium der Gattung Pseudomonas, transformiert mit einer rekombinanten DNA nach einem oder mehreren der Ansprüche 4-6.
- Bakterium nach Anspruch 7, worin das Bakterium Pseudomonas cepacia oder Pseudomonas putida ist.
- Verfahren zur Herstellung von Lipase, umfassend die Schritte der Transformation eines Bakteriums mit einer rekombinanten DNA nach Anspruch 4;Kultivierung des transformierten Bakteriums, so daß die Lipase sich in dem Kulturmedium anreichert; undGewinnung der Lipase aus dem Kulturmedium.
- Verfahren nach Anspruch 9, wobei die Transformation durch Elektroporation durchgeführt wird.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP45557/88 | 1988-02-28 | ||
| JP4555788 | 1988-02-28 | ||
| JP63300386A JP3079276B2 (ja) | 1988-02-28 | 1988-11-28 | 組換え体dna、それを含むシュードモナス属菌及びそれを用いたリパーゼの製造法 |
| JP300386/88 | 1988-11-28 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| EP0331376A2 EP0331376A2 (de) | 1989-09-06 |
| EP0331376A3 EP0331376A3 (de) | 1990-05-16 |
| EP0331376B1 true EP0331376B1 (de) | 1997-10-08 |
Family
ID=26385570
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP89301872A Expired - Lifetime EP0331376B1 (de) | 1988-02-28 | 1989-02-24 | Rekombinante DNA, sie enthaltendes Bakterium der Gattung Pseudomonas und ihre Verwendung zur Herstellung von Lipase |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US5290694A (de) |
| EP (1) | EP0331376B1 (de) |
| JP (1) | JP3079276B2 (de) |
| DE (1) | DE68928368T2 (de) |
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| US9045514B2 (en) | 2010-01-22 | 2015-06-02 | Dupont Nutrition Biosciences Aps | Methods for producing amino-substituted glycolipid compounds |
| US10570352B2 (en) | 2015-01-08 | 2020-02-25 | Stepan Company | Cold-water laundry detergents |
| US11920170B2 (en) | 2015-12-09 | 2024-03-05 | Danisco Us Inc. | Alpha-amylase combinatorial variants |
| US12480110B2 (en) | 2015-12-09 | 2025-11-25 | Danisco Us Inc. | Alpha-amylase combinatorial variants |
Also Published As
| Publication number | Publication date |
|---|---|
| DE68928368D1 (de) | 1997-11-13 |
| US5290694A (en) | 1994-03-01 |
| DE68928368T2 (de) | 1998-04-09 |
| EP0331376A3 (de) | 1990-05-16 |
| JPH0387187A (ja) | 1991-04-11 |
| EP0331376A2 (de) | 1989-09-06 |
| JP3079276B2 (ja) | 2000-08-21 |
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