ATE160176T1 - Verfahren zur herstellung eines proteins mittels eines durch mehrfachkopieintegrierung eines expressionsvektors transformierten pilzes - Google Patents

Verfahren zur herstellung eines proteins mittels eines durch mehrfachkopieintegrierung eines expressionsvektors transformierten pilzes

Info

Publication number
ATE160176T1
ATE160176T1 AT90911878T AT90911878T ATE160176T1 AT E160176 T1 ATE160176 T1 AT E160176T1 AT 90911878 T AT90911878 T AT 90911878T AT 90911878 T AT90911878 T AT 90911878T AT E160176 T1 ATE160176 T1 AT E160176T1
Authority
AT
Austria
Prior art keywords
protein
expression vector
integration
producing
yeast
Prior art date
Application number
AT90911878T
Other languages
English (en)
Inventor
Marco Luigi Frederic Giuseppin
Maria Teresa Santos Lopes
Roelf Johannes Planta
Johannes Maria Antoni Verbakel
Cornelis Theodorus Verrips
Original Assignee
Unilever Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB898915659A external-priority patent/GB8915659D0/en
Application filed by Unilever Nv filed Critical Unilever Nv
Application granted granted Critical
Publication of ATE160176T1 publication Critical patent/ATE160176T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2465—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on alpha-galactose-glycoside bonds, e.g. alpha-galactosidase (3.2.1.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
AT90911878T 1989-07-07 1990-07-09 Verfahren zur herstellung eines proteins mittels eines durch mehrfachkopieintegrierung eines expressionsvektors transformierten pilzes ATE160176T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB898915659A GB8915659D0 (en) 1989-07-07 1989-07-07 Enzymes,their production and use
EP90201007 1990-04-20

Publications (1)

Publication Number Publication Date
ATE160176T1 true ATE160176T1 (de) 1997-11-15

Family

ID=26125849

Family Applications (2)

Application Number Title Priority Date Filing Date
AT90911878T ATE160176T1 (de) 1989-07-07 1990-07-09 Verfahren zur herstellung eines proteins mittels eines durch mehrfachkopieintegrierung eines expressionsvektors transformierten pilzes
AT97200631T ATE196505T1 (de) 1989-07-07 1990-07-09 Verfahren zur herstellung eines proteins mittels eines durch mehrfachkopie-integrierung eines expressionsvektors tranformierten pilzes

Family Applications After (1)

Application Number Title Priority Date Filing Date
AT97200631T ATE196505T1 (de) 1989-07-07 1990-07-09 Verfahren zur herstellung eines proteins mittels eines durch mehrfachkopie-integrierung eines expressionsvektors tranformierten pilzes

Country Status (10)

Country Link
US (1) US6090574A (de)
EP (2) EP0481008B1 (de)
JP (1) JP3273609B2 (de)
AT (2) ATE160176T1 (de)
CA (1) CA2063592C (de)
DE (2) DE69033633T2 (de)
DK (2) DK0778348T3 (de)
ES (2) ES2150188T3 (de)
IE (1) IE81016B1 (de)
WO (1) WO1991000920A2 (de)

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JP3273609B2 (ja) 2002-04-08
EP0481008A1 (de) 1992-04-22
IE81016B1 (en) 1999-09-22
DE69033633D1 (de) 2000-10-26
CA2063592A1 (en) 1991-01-08
EP0481008B1 (de) 1997-11-12
IE902496A1 (en) 1991-02-13
CA2063592C (en) 2003-01-21
DE69031710D1 (de) 1997-12-18
DK0778348T3 (da) 2000-12-04
ES2150188T3 (es) 2000-11-16
WO1991000920A3 (en) 1991-03-07
WO1991000920A2 (en) 1991-01-24
ES2109238T3 (es) 1998-01-16
DK0481008T3 (da) 1998-05-04
US6090574A (en) 2000-07-18
ATE196505T1 (de) 2000-10-15
EP0778348B1 (de) 2000-09-20
EP0778348A1 (de) 1997-06-11
DE69033633T2 (de) 2001-05-03
DE69031710T2 (de) 1998-03-05
JPH05501949A (ja) 1993-04-15

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