ES2150188T3 - Procedimiento para la preparacion de una proteina mediante un hongo transformado por integracion multicopia de un vector de expresion. - Google Patents
Procedimiento para la preparacion de una proteina mediante un hongo transformado por integracion multicopia de un vector de expresion.Info
- Publication number
- ES2150188T3 ES2150188T3 ES97200631T ES97200631T ES2150188T3 ES 2150188 T3 ES2150188 T3 ES 2150188T3 ES 97200631 T ES97200631 T ES 97200631T ES 97200631 T ES97200631 T ES 97200631T ES 2150188 T3 ES2150188 T3 ES 2150188T3
- Authority
- ES
- Spain
- Prior art keywords
- protein
- multicopy integration
- integration
- expression
- vector
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2465—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on alpha-galactose-glycoside bonds, e.g. alpha-galactosidase (3.2.1.22)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
- C12N9/20—Triglyceride splitting, e.g. by means of lipase
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
SE DESCRIBE UN PROCEDIMIENTO PARA PREPARAR UNA PROTEINA MEDIANTE UNA EUCARIOTA TRANSFORMADA MEDIANTE INTEGRACION DE MULTIPLES COPIAS DE UN VECTOR DE EXPRESION EN EL GENOMA DE UNA LEVADURA, COMO SACCHAROMYCES, HANSENULA Y KLUYVEROMYCES, O DE UN MOLDE COMO ASPERGILLUS, RHIZOPUS Y TRICHODERMA, DICHO VECTOR DE EXPRESION CONTENIENDO TANTO UN "GEN EXPRESABLE" QUE CODIFICA DICHA PROTEINA COMO UN LLAMADO "MARCADOR DE SELECCION DEFICIENTE NECESARIO PARA LE CRECIMIENTO DE LA LEVADURA O MOLDE EN UN MEDIO ESPECIFICO", COMO EL GEN LEU2D, TRP1D O URA3D, EN COMBINACION CON UNA SECUENCIA DEL ADN RIBOSOMAL, DANDO COMO RESULTADO UNA INTEGRACION DE COPIA ELEVADA ESTABLE DE 100-300 COPIAS POR CELULA. ESTA INTEGRACION DE MULTIPLES COPIAS DA COMO RESULTADO UN AUMENTO DE PRODUCCION DE LA PROTEINA DESEADA, QUE PUEDE SER A-GALACTOSIDASA DE GUAR, UNA OXIDASA O UNA ENZIMA HIDROLITICA COMO UNA LIPASA.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB898915659A GB8915659D0 (en) | 1989-07-07 | 1989-07-07 | Enzymes,their production and use |
EP90201007 | 1990-04-20 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2150188T3 true ES2150188T3 (es) | 2000-11-16 |
Family
ID=26125849
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES97200631T Expired - Lifetime ES2150188T3 (es) | 1989-07-07 | 1990-07-09 | Procedimiento para la preparacion de una proteina mediante un hongo transformado por integracion multicopia de un vector de expresion. |
ES90911878T Expired - Lifetime ES2109238T3 (es) | 1989-07-07 | 1990-07-09 | Procedimiento para la preparacion de una proteina mediante un hongo transformado por integracion multicopia de un vector de expresion. |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES90911878T Expired - Lifetime ES2109238T3 (es) | 1989-07-07 | 1990-07-09 | Procedimiento para la preparacion de una proteina mediante un hongo transformado por integracion multicopia de un vector de expresion. |
Country Status (10)
Country | Link |
---|---|
US (1) | US6090574A (es) |
EP (2) | EP0778348B1 (es) |
JP (1) | JP3273609B2 (es) |
AT (2) | ATE196505T1 (es) |
CA (1) | CA2063592C (es) |
DE (2) | DE69033633T2 (es) |
DK (2) | DK0778348T3 (es) |
ES (2) | ES2150188T3 (es) |
IE (1) | IE81016B1 (es) |
WO (1) | WO1991000920A2 (es) |
Families Citing this family (99)
Publication number | Priority date | Publication date | Assignee | Title |
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WO1991014781A1 (en) * | 1990-03-19 | 1991-10-03 | Henkel Research Corporation | METHOD FOR INCREASING THE OMEGA-HYDROXYLASE ACTIVITY IN $i(CANDIDA TROPICALIS) |
JP3133774B2 (ja) * | 1990-10-04 | 2001-02-13 | 日本食品化工株式会社 | 新規な宿主−ベクター系 |
ATE140974T1 (de) * | 1990-10-04 | 1996-08-15 | Japan Maize Prod | Wirt-vektor-system |
IT1251435B (it) * | 1991-09-04 | 1995-05-09 | Sclavo Spa | Vettore genetico per la integrazione multipla stabile di sequenze di dna nel genoma dei lieviti kluyveromyces lactis e saccharomyces cerevisiae e plasmidi che lo contengono. |
WO1994013821A1 (en) * | 1992-12-11 | 1994-06-23 | Quest International B.V. | The use of the kluyveromyces marxianus inulinase gene promoter for protein production |
US6936289B2 (en) | 1995-06-07 | 2005-08-30 | Danisco A/S | Method of improving the properties of a flour dough, a flour dough improving composition and improved food products |
CA2265519A1 (en) * | 1996-09-24 | 1998-04-02 | Plant Genetic Systems N.V. | Dna-constructs comprising intergenic ribosomal dna and methods to produce proteins using these dna-constructs |
US7883872B2 (en) | 1996-10-10 | 2011-02-08 | Dyadic International (Usa), Inc. | Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose |
US6255115B1 (en) | 1997-04-07 | 2001-07-03 | Unilever Patent Holdings Bv | Agrobacterium mediated transformation of moulds, in particular those belonging to the genus Aspergillus |
EP0870835A1 (en) * | 1997-04-07 | 1998-10-14 | Unilever N.V. | Agrobacterium mediated transformation of moulds, in particular those belonging to the genus Aspergillus |
US6265186B1 (en) | 1997-04-11 | 2001-07-24 | Dsm N.V. | Yeast cells comprising at least two copies of a desired gene integrated into the chromosomal genome at more than one non-ribosomal RNA encoding domain, particularly with Kluyveromyces |
DK0979294T3 (en) * | 1997-04-11 | 2015-08-31 | Dsm Ip Assets Bv | Gene conversion AS A TOOL FOR CONSTRUCTION OF RECOMBINANT INDUSTRIAL filamentous fungi |
ES2308805T3 (es) * | 1997-04-11 | 2008-12-01 | Dsm Ip Assets B.V. | Celulas de levadura que comprenden al menos dos copias de un gen deseado integradas en el genoma cromosomico en mas de un domicio que codifica el arn no ribosomico particularmente con kluyveromyces. |
EP1015612B1 (en) * | 1997-05-27 | 2005-01-26 | Hanil Synthetic Fiber Co., Ltd. | Process for preparing recombinant proteins using highly efficient expression vector from saccharomyces cerevisiae |
US6228583B1 (en) * | 1997-08-04 | 2001-05-08 | Massachusetts Institute Of Technology | Assays for compounds which extend life span |
CN1284998A (zh) * | 1997-12-22 | 2001-02-21 | 尤尼利弗公司 | 多拷贝基因在霉菌中的定点整合方法 |
PL341760A1 (en) * | 1997-12-22 | 2001-05-07 | Dsm Nv | Expression cloning in mildew fungi |
WO1999055885A2 (en) * | 1998-04-28 | 1999-11-04 | Axys Pharmaceuticals, Inc. | Novel serine protease capable of selective cleavage of insulin-like growth factor binding protein |
AU771539C (en) | 1998-10-06 | 2005-01-13 | Dyadic International (Usa), Inc. | Transformation system in the field of filamentous fungal hosts |
US7220542B2 (en) | 2000-07-17 | 2007-05-22 | Van Den Brink Johannes Maarten | Expression cloning in filamentous fungi |
AU7398100A (en) * | 1999-09-13 | 2001-04-17 | Concordia University | A multifunctional system for the efficient manipulation of protein expression infilamentous fungi and method using same |
DE19963690A1 (de) * | 1999-11-23 | 2001-06-13 | Rhein Biotech Proz & Prod Gmbh | Vektoren und Verfahren zur Herstellung rekombinanter Proteine in Pilzen |
WO2002092757A2 (en) * | 2000-11-15 | 2002-11-21 | Kemin Industries, Inc. | Enzyme over-producing transgenic microorganisms |
ZA200303353B (en) * | 2000-12-13 | 2004-04-30 | Unilever Plc | Method for the production of a heterologous protein by a fungus. |
AU2002226307A1 (en) * | 2001-01-25 | 2002-08-06 | Evolva Biotech A/S | Artificial chromosomes comprising concatemers of expressible nucleotide sequences |
EP1356037B1 (en) | 2001-01-25 | 2011-03-09 | Evolva Ltd. | A library of a collection of cells |
AU2002339115B2 (en) | 2001-05-18 | 2007-03-15 | Dupont Nutrition Biosciences Aps | Method of preparing a dough with an enzyme |
ITMI20011728A1 (it) * | 2001-08-06 | 2003-02-06 | Consorzio Per Le Ricerche E Lo | Vettore per l'integrazione sito-specifica di sequenze di dna eterologhe in lieviti metilotrofi |
WO2004072225A2 (en) * | 2003-02-12 | 2004-08-26 | Ramot At Tel Aviv University Ltd. | Transgenic fungi expressing bcl-2 and methods of using bcl-2 or portions thereof for improving biomass production, survival, longevity, stress resistance and pathogenicity of fungi |
CN101818177A (zh) | 2004-07-16 | 2010-09-01 | 丹尼斯科公司 | 脂肪分解酶及其在食品工业中的应用 |
EP2102366A4 (en) | 2006-12-10 | 2010-01-27 | Dyadic International Inc | EXPRESSION AND HIGH-DROP SCREENING OF COMPLEX EXPRESSED DNA LIBRARIES IN FADENED MUSHROOMS |
EP2197893B1 (en) | 2007-09-07 | 2013-07-24 | Dyadic International, Inc. | Novel fungal enzymes |
US20110318795A1 (en) | 2007-11-20 | 2011-12-29 | Verwaal Rene | Dicarboxylic acid production in a filamentous fungus |
CN101896606B (zh) | 2007-11-20 | 2016-12-21 | 帝斯曼知识产权资产管理有限公司 | 在重组酵母中生产二羧酸 |
BRPI0819275B8 (pt) | 2007-11-20 | 2022-07-19 | Dsm Ip Assets Bv | Célula eucariótica recombinante produtora de ácido succínico e processo para a preparação de ácido succínico |
EP2116136A1 (en) | 2008-05-08 | 2009-11-11 | Nederlandse Organisatie voor toegepast- natuurwetenschappelijk onderzoek TNO | Novel phytases |
US8546645B2 (en) | 2008-10-03 | 2013-10-01 | Agrisoma Biosciences Inc. | Production of modified fatty acids in plants through rDNA targeted integration of heterologous genes |
EP2408907B8 (en) | 2009-03-17 | 2018-04-18 | Dutch DNA Biotech B.V. | Use of cellulose and ligno-cellulose active proteins |
US9353387B2 (en) | 2009-04-15 | 2016-05-31 | Dsm Ip Assets B.V. | Dicarboxylic acid production process |
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WO2011131667A1 (en) | 2010-04-21 | 2011-10-27 | Dsm Ip Assets B.V. | Cell suitable for fermentation of a mixed sugar composition |
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US9353376B2 (en) | 2010-10-13 | 2016-05-31 | Dsm Ip Assets, B.V. | Pentose and glucose fermenting yeast cell |
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EP2554668A1 (en) | 2011-08-04 | 2013-02-06 | DSM IP Assets B.V. | A pentose sugar fermenting cell |
AR087423A1 (es) | 2011-08-04 | 2014-03-19 | Dsm Ip Assets Bv | Celula capaz de fermentar azucares pentosas |
DK2806754T3 (en) | 2012-01-23 | 2019-02-18 | Dsm Ip Assets Bv | Diterpene PREPARATION |
US20130273619A1 (en) | 2012-04-16 | 2013-10-17 | Basf Se | Process for the Preparation of (3E, 7E)-Homofarnesol |
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EP2772545A1 (en) | 2013-03-01 | 2014-09-03 | Nederlandse Organisatie voor toegepast -natuurwetenschappelijk onderzoek TNO | Starch active proteins |
EP2992105A1 (en) | 2013-05-02 | 2016-03-09 | Dutch DNA Biotech B.V. | Novel organic acid pathway |
DK3004366T3 (da) | 2013-05-31 | 2019-05-20 | Dsm Ip Assets Bv | Mikroorganismer til diterpenproduktion |
CN108064135A (zh) | 2013-07-15 | 2018-05-22 | 帝斯曼知识产权资产管理有限公司 | 二萜的生产 |
DK3027632T3 (da) | 2013-07-31 | 2019-06-17 | Dsm Ip Assets Bv | Indvinding af steviolglycosider fra et fermenteringsmedium |
WO2016131818A1 (en) | 2015-02-16 | 2016-08-25 | Dsm Ip Assets B.V. | Process for producing itaconic acid under anaerobic conditions |
CA2980090A1 (en) | 2015-04-03 | 2016-10-06 | Dsm Ip Assets B.V. | Steviol glycosides |
WO2016172282A1 (en) | 2015-04-21 | 2016-10-27 | Dsm Ip Assets B.V. | Microbial production of terpenoids |
PL3298133T3 (pl) | 2015-05-22 | 2020-03-31 | Dsm Ip Assets B.V. | Komórki drożdży zużywające octan |
EP3320104B1 (en) | 2015-07-10 | 2021-11-10 | DSM IP Assets B.V. | Method for preparing a steviol glycoside composition |
CA2993744A1 (en) | 2015-08-13 | 2017-02-16 | Dsm Ip Assets B.V. | Steviol glycoside transport |
WO2017050652A1 (en) | 2015-09-25 | 2017-03-30 | Dsm Ip Assets B.V. | Asparaginase |
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US10787649B2 (en) | 2016-07-13 | 2020-09-29 | Dsm Ip Assets B.V. | Malate dehyrogenases |
BR112019002417B1 (pt) | 2016-08-09 | 2023-05-02 | Dsm Ip Assets B.V. | Cristalização de glicosídeos de esteviol |
WO2018029274A1 (en) | 2016-08-09 | 2018-02-15 | Dsm Ip Assets B.V. | Crystallization of steviol glycosides |
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WO2018073107A1 (en) | 2016-10-19 | 2018-04-26 | Dsm Ip Assets B.V. | Eukaryotic cell comprising xylose isomerase |
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EP3559222A1 (en) | 2016-12-21 | 2019-10-30 | DSM IP Assets B.V. | Lipolytic enzyme variants |
WO2018114995A1 (en) | 2016-12-22 | 2018-06-28 | Dsm Ip Assets B.V. | Fermentation process for producing steviol glycosides |
US11667886B2 (en) | 2017-06-13 | 2023-06-06 | Dsm Ip Assets B.V. | Recombinant yeast cell |
JP2020530271A (ja) * | 2017-06-30 | 2020-10-22 | ピーティーティー グローバル ケミカル パブリック カンパニー リミテッド | 安定化されたコピー数の機能性dna配列を有する微生物及び関連方法 |
US11414683B2 (en) | 2017-09-26 | 2022-08-16 | Dsm Ip Assets B.V. | Acetic acid consuming strain |
EP3533878A1 (en) | 2018-02-28 | 2019-09-04 | Dutch DNA Biotech B.V. | Process for producing citramalic acid employing aspergillus |
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CN111254127B (zh) * | 2018-11-30 | 2022-06-10 | 康码(上海)生物科技有限公司 | 蛋白激酶a催化亚基tpk2基因改造方法及其应用 |
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KR910002604B1 (ko) | 1986-05-28 | 1991-04-27 | 미쓰비시덴기 가부시기가이샤 | 영상 재생 장치 |
CA1339101C (en) * | 1986-06-03 | 1997-07-29 | Nicolaas Overbeeke | Production of guar alpha-galactosidase and immunologically related alpha-galactosidases by host organisms transformed with recombinant dna methods |
GB8629536D0 (en) | 1986-12-10 | 1987-01-21 | Unilever Plc | Enzymatic detergent composition |
PT88556B (pt) * | 1987-09-28 | 1992-11-30 | Smithkline Biolog | Processo de integracao genomica estavel e de expressao de uma sequencia de cadificacao estranha, em leveduras |
DK571587D0 (da) | 1987-11-02 | 1987-11-02 | Novo Industri As | Enzymatisk detergentsammensaetning |
EP0318775B1 (en) * | 1987-12-03 | 1994-02-02 | Chisso Corporation | A lipase gene |
US5001230A (en) * | 1988-02-18 | 1991-03-19 | Schering Corporation | T cell activation markers |
JP3079276B2 (ja) * | 1988-02-28 | 2000-08-21 | 天野製薬株式会社 | 組換え体dna、それを含むシュードモナス属菌及びそれを用いたリパーゼの製造法 |
JP2639677B2 (ja) * | 1988-03-14 | 1997-08-13 | 旭化成工業株式会社 | リパーゼの遺伝情報を有するdna |
-
1990
- 1990-07-09 AT AT97200631T patent/ATE196505T1/de not_active IP Right Cessation
- 1990-07-09 EP EP97200631A patent/EP0778348B1/en not_active Expired - Lifetime
- 1990-07-09 DK DK97200631T patent/DK0778348T3/da active
- 1990-07-09 WO PCT/EP1990/001138 patent/WO1991000920A2/en active IP Right Grant
- 1990-07-09 ES ES97200631T patent/ES2150188T3/es not_active Expired - Lifetime
- 1990-07-09 AT AT90911878T patent/ATE160176T1/de not_active IP Right Cessation
- 1990-07-09 DE DE69033633T patent/DE69033633T2/de not_active Expired - Lifetime
- 1990-07-09 EP EP90911878A patent/EP0481008B1/en not_active Expired - Lifetime
- 1990-07-09 DE DE69031710T patent/DE69031710T2/de not_active Expired - Lifetime
- 1990-07-09 IE IE249690A patent/IE81016B1/en not_active IP Right Cessation
- 1990-07-09 DK DK90911878.8T patent/DK0481008T3/da active
- 1990-07-09 ES ES90911878T patent/ES2109238T3/es not_active Expired - Lifetime
- 1990-07-09 JP JP51119190A patent/JP3273609B2/ja not_active Expired - Fee Related
- 1990-07-09 CA CA002063592A patent/CA2063592C/en not_active Expired - Lifetime
-
1994
- 1994-12-27 US US08/364,010 patent/US6090574A/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
DK0778348T3 (da) | 2000-12-04 |
IE81016B1 (en) | 1999-09-22 |
ATE160176T1 (de) | 1997-11-15 |
DE69031710T2 (de) | 1998-03-05 |
EP0481008A1 (en) | 1992-04-22 |
CA2063592A1 (en) | 1991-01-08 |
WO1991000920A3 (en) | 1991-03-07 |
WO1991000920A2 (en) | 1991-01-24 |
ES2109238T3 (es) | 1998-01-16 |
EP0778348B1 (en) | 2000-09-20 |
DE69033633D1 (de) | 2000-10-26 |
EP0481008B1 (en) | 1997-11-12 |
DE69031710D1 (de) | 1997-12-18 |
ATE196505T1 (de) | 2000-10-15 |
US6090574A (en) | 2000-07-18 |
JP3273609B2 (ja) | 2002-04-08 |
DE69033633T2 (de) | 2001-05-03 |
CA2063592C (en) | 2003-01-21 |
EP0778348A1 (en) | 1997-06-11 |
IE902496A1 (en) | 1991-02-13 |
DK0481008T3 (da) | 1998-05-04 |
JPH05501949A (ja) | 1993-04-15 |
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