ATE196505T1 - Verfahren zur herstellung eines proteins mittels eines durch mehrfachkopie-integrierung eines expressionsvektors tranformierten pilzes - Google Patents
Verfahren zur herstellung eines proteins mittels eines durch mehrfachkopie-integrierung eines expressionsvektors tranformierten pilzesInfo
- Publication number
- ATE196505T1 ATE196505T1 AT97200631T AT97200631T ATE196505T1 AT E196505 T1 ATE196505 T1 AT E196505T1 AT 97200631 T AT97200631 T AT 97200631T AT 97200631 T AT97200631 T AT 97200631T AT E196505 T1 ATE196505 T1 AT E196505T1
- Authority
- AT
- Austria
- Prior art keywords
- protein
- expression vector
- integration
- producing
- yeast
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2465—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on alpha-galactose-glycoside bonds, e.g. alpha-galactosidase (3.2.1.22)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
- C12N9/20—Triglyceride splitting, e.g. by means of lipase
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB898915659A GB8915659D0 (en) | 1989-07-07 | 1989-07-07 | Enzymes,their production and use |
EP90201007 | 1990-04-20 |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE196505T1 true ATE196505T1 (de) | 2000-10-15 |
Family
ID=26125849
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT97200631T ATE196505T1 (de) | 1989-07-07 | 1990-07-09 | Verfahren zur herstellung eines proteins mittels eines durch mehrfachkopie-integrierung eines expressionsvektors tranformierten pilzes |
AT90911878T ATE160176T1 (de) | 1989-07-07 | 1990-07-09 | Verfahren zur herstellung eines proteins mittels eines durch mehrfachkopieintegrierung eines expressionsvektors transformierten pilzes |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT90911878T ATE160176T1 (de) | 1989-07-07 | 1990-07-09 | Verfahren zur herstellung eines proteins mittels eines durch mehrfachkopieintegrierung eines expressionsvektors transformierten pilzes |
Country Status (10)
Country | Link |
---|---|
US (1) | US6090574A (de) |
EP (2) | EP0481008B1 (de) |
JP (1) | JP3273609B2 (de) |
AT (2) | ATE196505T1 (de) |
CA (1) | CA2063592C (de) |
DE (2) | DE69033633T2 (de) |
DK (2) | DK0481008T3 (de) |
ES (2) | ES2109238T3 (de) |
IE (1) | IE81016B1 (de) |
WO (1) | WO1991000920A2 (de) |
Families Citing this family (99)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1991014781A1 (en) * | 1990-03-19 | 1991-10-03 | Henkel Research Corporation | METHOD FOR INCREASING THE OMEGA-HYDROXYLASE ACTIVITY IN $i(CANDIDA TROPICALIS) |
ATE140974T1 (de) * | 1990-10-04 | 1996-08-15 | Japan Maize Prod | Wirt-vektor-system |
JP3133774B2 (ja) * | 1990-10-04 | 2001-02-13 | 日本食品化工株式会社 | 新規な宿主−ベクター系 |
IT1251435B (it) * | 1991-09-04 | 1995-05-09 | Sclavo Spa | Vettore genetico per la integrazione multipla stabile di sequenze di dna nel genoma dei lieviti kluyveromyces lactis e saccharomyces cerevisiae e plasmidi che lo contengono. |
WO1994013821A1 (en) * | 1992-12-11 | 1994-06-23 | Quest International B.V. | The use of the kluyveromyces marxianus inulinase gene promoter for protein production |
US6936289B2 (en) | 1995-06-07 | 2005-08-30 | Danisco A/S | Method of improving the properties of a flour dough, a flour dough improving composition and improved food products |
EP0932691A1 (de) * | 1996-09-24 | 1999-08-04 | Plant Genetic Systems N.V. | Dna-konstrukte, die intergenische ribosomale dna enthalten, und deren verwendung zur proteinherstellung |
US7883872B2 (en) | 1996-10-10 | 2011-02-08 | Dyadic International (Usa), Inc. | Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose |
EP0870835A1 (de) * | 1997-04-07 | 1998-10-14 | Unilever N.V. | Transformation von Schimmeln durch Agrobacterium, insbesondere von der Gattung Aspergillus |
BR9807941A (pt) * | 1997-04-07 | 2000-02-22 | Unilever Nv | Processos para a produção e para cultivar um bolor transformado, e, bolor transformado |
US6432672B1 (en) | 1997-04-11 | 2002-08-13 | Gerardus Cornelis Maria Selten | Gene conversion as a tool for the construction of recombinant industrial filamentous fungi |
US6265186B1 (en) | 1997-04-11 | 2001-07-24 | Dsm N.V. | Yeast cells comprising at least two copies of a desired gene integrated into the chromosomal genome at more than one non-ribosomal RNA encoding domain, particularly with Kluyveromyces |
ATE398181T1 (de) * | 1997-04-11 | 2008-07-15 | Dsm Ip Assets Bv | Hefezellen welche zumindest zwei kopien von einem erwünschten gens enthalt und integrierte in dem chromosomal genom auf mehr als einem nicht- ribosomaler rns kodierende bereich, ins besonders mit kluyveromyces |
EP1015612B1 (de) * | 1997-05-27 | 2005-01-26 | Hanil Synthetic Fiber Co., Ltd. | Verfahren zur herstellung von rekombinanten eiweissen durch verwendung von hocheffizienten expressionsvektoren aus saccharomyces cerevisiae |
US6228583B1 (en) * | 1997-08-04 | 2001-05-08 | Massachusetts Institute Of Technology | Assays for compounds which extend life span |
DE69838106T2 (de) * | 1997-12-22 | 2008-04-03 | Dsm Ip Assets B.V. | Expressionsklonierung in filamentösen pilzen |
US6436643B1 (en) | 1997-12-22 | 2002-08-20 | Unilever Patent Holdings Bv | Process for site-directed integration of multiple copies of a gene in a mould |
AU3669499A (en) * | 1998-04-28 | 1999-11-16 | Axys Pharmaceuticals, Inc. | Novel serine protease capable of selective cleavage of insulin-like growth factor binding protein |
EA005682B1 (ru) | 1998-10-06 | 2005-04-28 | Марк Аарон Эмалфарб | Трансформированные грибы, в частности рода chrysosporium, способные к синтезу гетерологичных полипептидов |
US7220542B2 (en) | 2000-07-17 | 2007-05-22 | Van Den Brink Johannes Maarten | Expression cloning in filamentous fungi |
AU7398100A (en) * | 1999-09-13 | 2001-04-17 | Concordia University | A multifunctional system for the efficient manipulation of protein expression infilamentous fungi and method using same |
DE19963690A1 (de) * | 1999-11-23 | 2001-06-13 | Rhein Biotech Proz & Prod Gmbh | Vektoren und Verfahren zur Herstellung rekombinanter Proteine in Pilzen |
AU2001297819A1 (en) * | 2000-11-15 | 2002-11-25 | Kemin Industries, Inc. | Enzyme over-producing transgenic microorganisms |
DE60126148T2 (de) * | 2000-12-13 | 2007-05-31 | Unilever N.V. | Verfahren zur herstellung von heterologen proteinen in fungi |
AU2002227883A1 (en) | 2001-01-25 | 2002-08-06 | Evolva Biotech A/S | A library of a collection of cells |
WO2002059330A2 (en) * | 2001-01-25 | 2002-08-01 | Evolva Biotech A/S | Artificial chromosomes comprising concatemers of expressible nucleotide sequences |
DE60220155T2 (de) | 2001-05-18 | 2008-02-07 | Danisco A/S | Verfahren zur herstellung von teig mit einem enzym |
ITMI20011728A1 (it) * | 2001-08-06 | 2003-02-06 | Consorzio Per Le Ricerche E Lo | Vettore per l'integrazione sito-specifica di sequenze di dna eterologhe in lieviti metilotrofi |
WO2004072225A2 (en) * | 2003-02-12 | 2004-08-26 | Ramot At Tel Aviv University Ltd. | Transgenic fungi expressing bcl-2 and methods of using bcl-2 or portions thereof for improving biomass production, survival, longevity, stress resistance and pathogenicity of fungi |
AU2005263954B2 (en) | 2004-07-16 | 2011-04-07 | Dupont Nutrition Biosciences Aps | Enzymatic oil-degumming method |
EP2505651A3 (de) | 2006-12-10 | 2013-01-09 | Dyadic International, Inc. | Pilzisolate mit reduzierter Proteaseaktivität |
US8551751B2 (en) | 2007-09-07 | 2013-10-08 | Dyadic International, Inc. | BX11 enzymes having xylosidase activity |
EP2222854A1 (de) | 2007-11-20 | 2010-09-01 | DSM IP Assets B.V. | Dicarbonsäureproduktion in einem fadenpilz |
BRPI0819273B8 (pt) | 2007-11-20 | 2022-07-19 | Dsm Ip Assets Bv | Célula microbiana eucariótica recombinante e processo para a preparação de um ácido dicarboxílico |
CN101896606B (zh) | 2007-11-20 | 2016-12-21 | 帝斯曼知识产权资产管理有限公司 | 在重组酵母中生产二羧酸 |
EP2116136A1 (de) | 2008-05-08 | 2009-11-11 | Nederlandse Organisatie voor toegepast- natuurwetenschappelijk onderzoek TNO | Neue Phytasen |
BRPI0913818A2 (pt) * | 2008-10-03 | 2017-03-28 | Agrisoma Biosciences Inc | método de produção de plantas transgênicas, planta transgênica e método de produção de óleo |
US8735100B2 (en) | 2009-03-17 | 2014-05-27 | Nederlandse Organisatie Voor Toegepast -Natuurwetenschappelijk Onderzoek Tno | Cellulose and ligno-cellulose active proteins |
EP2419518A1 (de) | 2009-04-15 | 2012-02-22 | DSM IP Assets B.V. | Dicarbonsäureherstellungsverfahren |
UA108853C2 (uk) | 2009-07-10 | 2015-06-25 | Спосіб ферментації галактози | |
DE102009035671A1 (de) * | 2009-07-30 | 2011-02-03 | Dechema Gesellschaft Für Chemische Technik Und Biotechnologie E.V. | Verfahren zur Expression von homologen Proteinen und/oder Peptiden in Pilzen der Klasse Dothideomycetes |
CN102869766B (zh) | 2010-04-21 | 2015-11-25 | 帝斯曼知识产权资产管理有限公司 | 适于发酵混合的糖组合物的细胞 |
EP2388331A1 (de) | 2010-05-21 | 2011-11-23 | Nederlandse Organisatie voor toegepast -natuurwetenschappelijk onderzoek TNO | Konstitutiver Promoter |
MY160995A (en) | 2010-10-13 | 2017-03-28 | Dsm Ip Assets Bv | Polypeptides with permease activity |
US20140141473A1 (en) | 2011-04-22 | 2014-05-22 | Dsm Ip Assets B.V. | Yeast cell capable of converting sugars including arabinose and xlose |
EP2554668A1 (de) | 2011-08-04 | 2013-02-06 | DSM IP Assets B.V. | Zelle zur Fermentierung von Pentosezucker |
AR087423A1 (es) | 2011-08-04 | 2014-03-19 | Dsm Ip Assets Bv | Celula capaz de fermentar azucares pentosas |
CN104203005A (zh) | 2012-01-23 | 2014-12-10 | 帝斯曼知识产权资产管理有限公司 | 二萜的生产 |
US20130273619A1 (en) | 2012-04-16 | 2013-10-17 | Basf Se | Process for the Preparation of (3E, 7E)-Homofarnesol |
DK2909305T3 (en) | 2012-10-16 | 2019-04-15 | Dsm Ip Assets Bv | CELLS WITH IMPROVED PENTOSE CONVERSION |
EP2772545A1 (de) | 2013-03-01 | 2014-09-03 | Nederlandse Organisatie voor toegepast -natuurwetenschappelijk onderzoek TNO | Stärkeaktive Proteine |
CA2911170A1 (en) | 2013-05-02 | 2014-11-06 | Dutch Dna Biotech B.V. | Novel organic acid pathway |
EP3502264A3 (de) | 2013-05-31 | 2019-11-06 | DSM IP Assets B.V. | Mikroorganismen zur herstellung von diterpen |
EP3885443A1 (de) | 2013-07-15 | 2021-09-29 | DSM IP Assets B.V. | Production de diterpène |
US20160185813A1 (en) | 2013-07-31 | 2016-06-30 | Dsm Ip Assets B.V. | Recovery of steviol glycosides |
CN107429273A (zh) | 2015-02-16 | 2017-12-01 | 帝斯曼知识产权资产管理有限公司 | 用于在厌氧条件下生产衣康酸的方法 |
CN113683712B (zh) | 2015-04-03 | 2022-10-21 | 帝斯曼知识产权资产管理有限公司 | 甜菊醇糖苷 |
EP3286303A1 (de) | 2015-04-21 | 2018-02-28 | DSM IP Assets B.V. | Mikrobielle herstellung von terpenoiden |
ES2755680T3 (es) | 2015-05-22 | 2020-04-23 | Dsm Ip Assets Bv | Célula de levadura que consume acetato |
EP4043579A1 (de) | 2015-07-10 | 2022-08-17 | DSM IP Assets B.V. | Verfahren zur herstellung einer steviolglycosidzusammensetzung |
CN107922913B (zh) | 2015-08-13 | 2022-04-05 | 帝斯曼知识产权资产管理有限公司 | 甜菊醇糖苷转运 |
WO2017050652A1 (en) | 2015-09-25 | 2017-03-30 | Dsm Ip Assets B.V. | Asparaginase |
US11136600B2 (en) | 2015-10-06 | 2021-10-05 | Dsm Ip Assets B.V. | Eukaryotic cell with increased production of fermentation product |
EP3435770A1 (de) | 2016-03-31 | 2019-02-06 | DSM IP Assets B.V. | Herstellung von milch mit geringem laktosegehalt |
WO2017167848A1 (en) | 2016-03-31 | 2017-10-05 | Dsm Ip Assets B.V. | Enzyme composition and preparation of a dairy product with improved properties |
EP3435772A1 (de) | 2016-03-31 | 2019-02-06 | DSM IP Assets B.V. | Enzymzusammensetzung und herstellung eines milchprodukts mit verbesserten eigenschaften |
US10689670B2 (en) | 2016-06-14 | 2020-06-23 | Dsm Ip Assets B.V. | Recombinant yeast cell |
WO2018011161A1 (en) | 2016-07-13 | 2018-01-18 | Dsm Ip Assets B.V. | Malate dehyrogenases |
US11878992B2 (en) | 2016-08-09 | 2024-01-23 | Dsm Ip Assets B.V. | Crystallization of steviol glycosides |
WO2018029274A1 (en) | 2016-08-09 | 2018-02-15 | Dsm Ip Assets B.V. | Crystallization of steviol glycosides |
CN110036114B (zh) | 2016-08-26 | 2023-09-29 | 勒萨弗尔公司 | 提高的衣康酸生产 |
WO2018073107A1 (en) | 2016-10-19 | 2018-04-26 | Dsm Ip Assets B.V. | Eukaryotic cell comprising xylose isomerase |
US10918113B2 (en) | 2016-12-21 | 2021-02-16 | Dsm Ip Assets B.V. | Lipolytic enzyme variants |
WO2018114938A1 (en) | 2016-12-21 | 2018-06-28 | Dsm Ip Assets B.V. | Lipolytic enzyme variants |
EP3559221A1 (de) | 2016-12-21 | 2019-10-30 | DSM IP Assets B.V. | Varianten lipolytischer enzyme |
WO2018114912A1 (en) | 2016-12-21 | 2018-06-28 | Dsm Ip Assets B.V. | Lipolytic enzyme variants |
WO2018114995A1 (en) | 2016-12-22 | 2018-06-28 | Dsm Ip Assets B.V. | Fermentation process for producing steviol glycosides |
CA3064143A1 (en) | 2017-06-13 | 2018-12-20 | Dsm Ip Assets B.V. | Recombinant yeast cell |
WO2019006312A1 (en) * | 2017-06-30 | 2019-01-03 | Myriant Corporation | MICROORGANISM HAVING A NUMBER OF STABILIZED COPIES OF A FUNCTIONAL DNA SEQUENCE AND ASSOCIATED METHODS |
US11414683B2 (en) | 2017-09-26 | 2022-08-16 | Dsm Ip Assets B.V. | Acetic acid consuming strain |
EP3533878A1 (de) | 2018-02-28 | 2019-09-04 | Dutch DNA Biotech B.V. | Verfahren zur herstellung von 2-hydroxy-2-methylbutandisäure unter verwendung von aspergillus |
WO2019243312A1 (en) | 2018-06-19 | 2019-12-26 | Dsm Ip Assets B.V. | Lipolytic enzyme variants |
CN111254128A (zh) * | 2018-11-30 | 2020-06-09 | 康码(上海)生物科技有限公司 | 蛋白激酶a催化亚基tpk1基因改造方法及其应用 |
CN111254127B (zh) * | 2018-11-30 | 2022-06-10 | 康码(上海)生物科技有限公司 | 蛋白激酶a催化亚基tpk2基因改造方法及其应用 |
CA3198626A1 (en) | 2020-10-22 | 2022-04-28 | Dsm Ip Assets B.V. | Microorganisms for diterpene production |
FR3122436A1 (fr) * | 2021-04-30 | 2022-11-04 | IFP Energies Nouvelles | Insertion multicopies d’un gène d’intérêt dans le génome d’un champignon |
CA3225417A1 (en) | 2021-06-24 | 2022-12-29 | Fonterra Co-Operative Group Limited | Recombinant proteins |
WO2023285281A1 (en) | 2021-07-12 | 2023-01-19 | Dsm Ip Assets B.V. | Recombinant yeast cell |
WO2023285279A1 (en) | 2021-07-12 | 2023-01-19 | Dsm Ip Assets B.V. | Recombinant yeast cell |
WO2023285294A1 (en) | 2021-07-12 | 2023-01-19 | Dsm Ip Assets B.V. | Recombinant yeast cell |
WO2023285280A1 (en) | 2021-07-12 | 2023-01-19 | Dsm Ip Assets B.V. | Recombinant yeast cell |
EP4370651A1 (de) | 2021-07-12 | 2024-05-22 | Danisco Us Inc | Rekombinante hefezelle |
EP4370691A1 (de) | 2021-07-12 | 2024-05-22 | Danisco US Inc. | Rekombinante hefezelle |
CN113564206B (zh) * | 2021-07-29 | 2023-09-26 | 山东大学 | 一种目的基因多拷贝整合到酿酒酵母染色体rDNA的方法 |
WO2023079049A1 (en) | 2021-11-04 | 2023-05-11 | Dsm Ip Assets B.V. | Variant polypeptide and recombinant yeast cell |
WO2023079050A1 (en) | 2021-11-04 | 2023-05-11 | Dsm Ip Assets B.V. | Recombinant yeast cell |
WO2023079048A1 (en) | 2021-11-04 | 2023-05-11 | Dsm Ip Assets B.V. | Process for the production of ethanol and recombinant yeast cell |
CA3237662A1 (en) | 2021-11-09 | 2023-05-19 | Amgen Inc. | Production of therapeutic proteins |
WO2023222614A1 (en) | 2022-05-16 | 2023-11-23 | Dsm Ip Assets B.V. | Lipolytic enzyme variants |
Family Cites Families (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU600885B2 (en) * | 1984-05-25 | 1990-08-30 | Zymogenetics Inc. | Stable DNA constructs |
GB8415186D0 (en) * | 1984-06-14 | 1984-07-18 | Ciba Geigy Ag | Polypeptides |
EP0173378B1 (de) * | 1984-07-27 | 1991-06-12 | Unilever N.V. | Verfahren zur Herstellung von einem Polypeptid mittels eines transformierten Mikroorganismus, ein dazu geeigneter transformierter Mikroorganismus, und zur Herstellung dieser Mikroorganismen geeignete DNS Sequenzen |
JPS61134325A (ja) * | 1984-12-04 | 1986-06-21 | Teijin Ltd | ハイブリツド抗体遺伝子の発現方法 |
JPH0614865B2 (ja) * | 1985-12-13 | 1994-03-02 | 麒麟麦酒株式会社 | α―アセト乳酸脱炭酸酵素をコードするDNA鎖およびこのDNA鎖により形質転換された酵母 |
KR910002604B1 (ko) | 1986-05-28 | 1991-04-27 | 미쓰비시덴기 가부시기가이샤 | 영상 재생 장치 |
CA1339101C (en) * | 1986-06-03 | 1997-07-29 | Nicolaas Overbeeke | Production of guar alpha-galactosidase and immunologically related alpha-galactosidases by host organisms transformed with recombinant dna methods |
GB8629536D0 (en) | 1986-12-10 | 1987-01-21 | Unilever Plc | Enzymatic detergent composition |
PT88556B (pt) * | 1987-09-28 | 1992-11-30 | Smithkline Biolog | Processo de integracao genomica estavel e de expressao de uma sequencia de cadificacao estranha, em leveduras |
DK571587D0 (da) | 1987-11-02 | 1987-11-02 | Novo Industri As | Enzymatisk detergentsammensaetning |
DE3887656T2 (de) * | 1987-12-03 | 1994-06-16 | Chisso Corp | Lipasegen. |
US5001230A (en) * | 1988-02-18 | 1991-03-19 | Schering Corporation | T cell activation markers |
JP3079276B2 (ja) * | 1988-02-28 | 2000-08-21 | 天野製薬株式会社 | 組換え体dna、それを含むシュードモナス属菌及びそれを用いたリパーゼの製造法 |
JP2639677B2 (ja) * | 1988-03-14 | 1997-08-13 | 旭化成工業株式会社 | リパーゼの遺伝情報を有するdna |
-
1990
- 1990-07-09 JP JP51119190A patent/JP3273609B2/ja not_active Expired - Fee Related
- 1990-07-09 IE IE249690A patent/IE81016B1/en not_active IP Right Cessation
- 1990-07-09 AT AT97200631T patent/ATE196505T1/de not_active IP Right Cessation
- 1990-07-09 ES ES90911878T patent/ES2109238T3/es not_active Expired - Lifetime
- 1990-07-09 EP EP90911878A patent/EP0481008B1/de not_active Expired - Lifetime
- 1990-07-09 DK DK90911878.8T patent/DK0481008T3/da active
- 1990-07-09 DK DK97200631T patent/DK0778348T3/da active
- 1990-07-09 WO PCT/EP1990/001138 patent/WO1991000920A2/en active IP Right Grant
- 1990-07-09 AT AT90911878T patent/ATE160176T1/de not_active IP Right Cessation
- 1990-07-09 DE DE69033633T patent/DE69033633T2/de not_active Expired - Lifetime
- 1990-07-09 CA CA002063592A patent/CA2063592C/en not_active Expired - Lifetime
- 1990-07-09 ES ES97200631T patent/ES2150188T3/es not_active Expired - Lifetime
- 1990-07-09 EP EP97200631A patent/EP0778348B1/de not_active Expired - Lifetime
- 1990-07-09 DE DE69031710T patent/DE69031710T2/de not_active Expired - Lifetime
-
1994
- 1994-12-27 US US08/364,010 patent/US6090574A/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
DE69033633T2 (de) | 2001-05-03 |
ATE160176T1 (de) | 1997-11-15 |
CA2063592A1 (en) | 1991-01-08 |
EP0481008B1 (de) | 1997-11-12 |
EP0778348B1 (de) | 2000-09-20 |
IE81016B1 (en) | 1999-09-22 |
WO1991000920A3 (en) | 1991-03-07 |
DK0481008T3 (da) | 1998-05-04 |
EP0778348A1 (de) | 1997-06-11 |
DE69033633D1 (de) | 2000-10-26 |
WO1991000920A2 (en) | 1991-01-24 |
ES2150188T3 (es) | 2000-11-16 |
EP0481008A1 (de) | 1992-04-22 |
IE902496A1 (en) | 1991-02-13 |
DK0778348T3 (da) | 2000-12-04 |
ES2109238T3 (es) | 1998-01-16 |
JP3273609B2 (ja) | 2002-04-08 |
DE69031710T2 (de) | 1998-03-05 |
JPH05501949A (ja) | 1993-04-15 |
CA2063592C (en) | 2003-01-21 |
US6090574A (en) | 2000-07-18 |
DE69031710D1 (de) | 1997-12-18 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
ATE196505T1 (de) | Verfahren zur herstellung eines proteins mittels eines durch mehrfachkopie-integrierung eines expressionsvektors tranformierten pilzes | |
ATE125865T1 (de) | Rekombinante humicola-lipase und verfahren zur herstellung von rekombinanten humicola-lipasen. | |
DE69334327D1 (de) | Verfahren zur Herstellung sekretierter Proteine | |
DE69939947D1 (de) | Verfahren zur herstellung von polypeptiden in mutierten aspergillus zellen | |
DE69720026D1 (de) | Verfahren zur herstellung von d-aminosäuren durch direkte gärung | |
DE3584149D1 (de) | Verfahren zur herstellung von alkohol. | |
DK684388D0 (da) | Coekspression i eukaryote celler | |
DE69231137T2 (de) | Verfahren zur Herstellung von Peroxidasen in einem Aspergillusstamm | |
EP0438200A1 (de) | Verfahren zur Expression heterologischer Gene in der Hefe Pichia Pastoris, Expressionsvektoren und transformierte Mikroorganismen | |
ATE451469T1 (de) | In vivo-verfahren zur herstellung von proteinen, die nicht-konventionelle aminosäuren enthalten | |
DE3882455D1 (de) | Verfahren zur herstellung von proteinen in loeslicher form. | |
DE69409354T2 (de) | Dextranase, Herstellungsverfahren und dafür kodierende DNS | |
CA2286395A1 (en) | Production method of heterologous protein | |
EP1262551A3 (de) | Sorbitol-Dehydrogenase Gen, rekombinante DNA, und Verfahren zur Herstellung von Sorbitol-Dehydrogenase | |
ATE122097T1 (de) | Expressionsvektor und verfahren zur regulierbaren herstellung von proteinen in eukaryonten. | |
ATE212669T1 (de) | Verfahren zur herstellung einer substanz | |
KR940021729A (ko) | 효모로부터 재조합 히루딘을 생산하는 방법 | |
ES8602933A1 (es) | Un metodo para clonar por tecnicas de dna recombinante penicilinacilasas e incrementar su produccion por fermentacion |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
UEP | Publication of translation of european patent specification | ||
EELA | Cancelled due to lapse of time |