BR9807941A - Processos para a produção e para cultivar um bolor transformado, e, bolor transformado - Google Patents

Processos para a produção e para cultivar um bolor transformado, e, bolor transformado

Info

Publication number
BR9807941A
BR9807941A BR9807941-7A BR9807941A BR9807941A BR 9807941 A BR9807941 A BR 9807941A BR 9807941 A BR9807941 A BR 9807941A BR 9807941 A BR9807941 A BR 9807941A
Authority
BR
Brazil
Prior art keywords
transformation
conidia
gene
awamori
aspergillus
Prior art date
Application number
BR9807941-7A
Other languages
English (en)
Inventor
Alida Godelieve Beijersbergen
Paul Bundok
Robertus Johannes Gouka
Marcellus Johannes Augus Groot
Paul Jan Jacob Hooykaas
Original Assignee
Unilever Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP97201022A external-priority patent/EP0870835A1/en
Application filed by Unilever Nv filed Critical Unilever Nv
Publication of BR9807941A publication Critical patent/BR9807941A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi

Abstract

"PROCESSOS PARA A PRODUçãO DE UM BOLOR TRANSFORMADO, E, BOLOR TRANSFORMADO". A invenção refere-se à transformação de bolores mediada por Agrobacterium que compreendem espécies das subdivisões fungais Ascomycotina, Basidiomucotina, Deuteromycotina, Mastigomycotina e Zygomuycotina, Exemplos demonstram a transformação de Aspergillus Awamori (tanto de protoplastos como de conídios), de Aspergillus nidulans, de Aspergillus niger, de Colletotrichum gloeosporioides, de Fusarium solanii pisi, de Neurospora crassa, de Trichoderma reesei, de Pleurotus ostreatus e de Agaricus bisporus (todos de conídios) e de Fusarium graminearum (tanto de conídios como de material ATCC seco por congelamento reidratado). Especialmente para Aspergillus awamori a freq³ência de transformação é muito mais alta do que com as técnicas convencionais de transformação de bolor. Foi ainda descoberto que não apenas um gene que pode ser expressado pode ser introduzido nestes bolores, porém até mesmo múltiplas cópias de tal gene, que, além disso, podem ser direcionadas por exemplo ao locus pyrG cromossomal, como exemplificado para A. awamori. Estas múltiplas cópias podem ser de um gene que codifica uma proteína desejada, homóloga ou heteróloga.
BR9807941-7A 1997-04-07 1998-03-24 Processos para a produção e para cultivar um bolor transformado, e, bolor transformado BR9807941A (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP97201022A EP0870835A1 (en) 1997-04-07 1997-04-07 Agrobacterium mediated transformation of moulds, in particular those belonging to the genus Aspergillus
EP97204062 1997-12-22
PCT/EP1998/001914 WO1998045455A1 (en) 1997-04-07 1998-03-24 Agrobacterium mediated transformation of moulds, in particular those belonging to the genus aspergillus

Publications (1)

Publication Number Publication Date
BR9807941A true BR9807941A (pt) 2000-02-22

Family

ID=26146337

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9807941-7A BR9807941A (pt) 1997-04-07 1998-03-24 Processos para a produção e para cultivar um bolor transformado, e, bolor transformado

Country Status (12)

Country Link
US (1) US6255115B1 (pt)
EP (1) EP0973917B1 (pt)
JP (1) JP4307563B2 (pt)
CN (1) CN1151264C (pt)
AU (1) AU7428398A (pt)
BR (1) BR9807941A (pt)
CA (1) CA2286307A1 (pt)
DE (1) DE69822142T2 (pt)
ES (1) ES2216284T3 (pt)
ID (1) ID22929A (pt)
MY (1) MY121328A (pt)
WO (1) WO1998045455A1 (pt)

Families Citing this family (65)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6015703A (en) * 1998-03-10 2000-01-18 Iogen Corporation Genetic constructs and genetically modified microbes for enhanced production of beta-glucosidase
US7220542B2 (en) 2000-07-17 2007-05-22 Van Den Brink Johannes Maarten Expression cloning in filamentous fungi
EP1238090B1 (en) 1999-12-16 2007-05-09 CropDesign N.V. Optimized t-dna transfer and vectors therefor
CA2452183C (en) 2000-06-28 2013-12-24 The Penn State Research Foundation Methods and compositions for highly effective transformation of filamentous fungi
EP2305800B1 (en) 2002-08-16 2015-05-27 Danisco US Inc. Novel variant hyprocrea jecorina CBH1 cellulases
CN1688198A (zh) * 2002-09-10 2005-10-26 金克克国际有限公司 使用高浓度糖混合物诱导基因表达
US7208585B2 (en) * 2002-09-18 2007-04-24 Genencor International, Inc. Protein purification
WO2004072225A2 (en) * 2003-02-12 2004-08-26 Ramot At Tel Aviv University Ltd. Transgenic fungi expressing bcl-2 and methods of using bcl-2 or portions thereof for improving biomass production, survival, longevity, stress resistance and pathogenicity of fungi
WO2005028636A2 (en) * 2003-03-21 2005-03-31 Genencor International, Inc. Novel cbh1 homologs and variant cbh1 cellulases
CN101410520B (zh) * 2003-04-29 2012-05-30 金克克国际有限公司 新颖的杆菌029cel纤维素酶
JP4757191B2 (ja) * 2003-04-30 2011-08-24 ジェネンコー・インターナショナル・インク 新規なバチルスｍＨＫｃｅｌセルラーゼ
WO2004099369A2 (en) * 2003-04-30 2004-11-18 Genencor International, Inc. Novel bacillus bagcel cellulase
EP1625208A4 (en) * 2003-05-12 2006-10-18 Genencor Int NEW LIPOLYTIC ENZYME LIP2
US7511005B2 (en) * 2003-05-12 2009-03-31 Danisco Us Inc., Genencor Division Lipolytic enzyme elip
EP1625202A4 (en) * 2003-05-12 2010-10-20 Genencor Int NEW LIPOLYTIC ENZYME LIP1
US20050181509A1 (en) * 2004-02-12 2005-08-18 The Penn State Research Foundation Dual selection based, targeted gene disruption method for fungi and fungus-like organisms
GB0405659D0 (en) * 2004-03-12 2004-04-21 Horticulture Res Internat Altered expression in filamentous fungi
US8097445B2 (en) 2004-03-25 2012-01-17 Danisco Us Inc. Exo-endo cellulase fusion protein
WO2005093050A2 (en) 2004-03-25 2005-10-06 Genencor International, Inc. Cellulase fusion protein and heterologous cellulase fusion construct encoding the same
WO2006071598A1 (en) * 2004-12-23 2006-07-06 Genencor International, Inc. Neutral cellulase catalytic core and method of producing same
EP1831362B1 (en) * 2004-12-30 2011-10-26 Genencor International, Inc. Acid fungal proteases
CA2592550C (en) 2004-12-30 2015-05-19 Genencor International, Inc. Novel variant hypocrea jecorina cbh2 cellulases
WO2006122023A1 (en) 2005-05-09 2006-11-16 The Samuel Roberts Noble Foundation, Inc. Agrobacterium transformation of stolons
CN100370016C (zh) * 2006-04-21 2008-02-20 贵州大学 对根癌农杆菌有抑制作用的黑曲霉菌株及其用途
US7968691B2 (en) 2006-08-23 2011-06-28 Danisco Us Inc. Pullulanase variants with increased productivity
US8216844B2 (en) * 2006-08-29 2012-07-10 Danisco Us Inc. Compositions and methods for improved protein production
US20100093061A1 (en) * 2006-12-20 2010-04-15 Bodie Elizabeth A Assays for Improved Fungal Strains
WO2008150376A1 (en) * 2007-05-21 2008-12-11 Danisco Us, Inc., Genencor Division Use of an aspartic protease (nsp24) signal sequence for heterologous protein expression
US20090221030A1 (en) * 2007-11-01 2009-09-03 Kai Bao Signal sequences and co-expressed chaperones for improving protein production in a host cell
US20120270302A1 (en) * 2011-04-25 2012-10-25 Eben Bayer Method for Making Dehydrated Mycelium Elements and Product Made Thereby
US7973215B2 (en) 2008-04-11 2011-07-05 Mycomagic Biotechnology Co., Ltd. Method for the introduction of a heterologous polynucleotide into a mushroom
EP2626422A1 (en) 2008-06-06 2013-08-14 Danisco US Inc. Compositions and methods comprising cellulase variants with reduced affinity to non-cellulosic materials
US8907165B2 (en) 2009-04-22 2014-12-09 Medicine In Need Corporation Production of provitamin A carotenoids in mushrooms and uses thereof
KR20120062643A (ko) 2009-06-03 2012-06-14 다니스코 유에스 인크. 향상된 발현, 활성 및/또는 안정성을 갖는 셀룰라아제 변이체, 및 이의 용도
IN2012DN03825A (pt) 2009-11-20 2015-08-28 Danisco Us Inc
BR112012030746A2 (pt) 2010-06-03 2016-02-16 Danisco Us Inc cepas hospedeiras fúngigas filamentosas e constructos de dna , e métodos de uso dos mesmos.
WO2011158623A1 (ja) * 2010-06-17 2011-12-22 キッコーマン株式会社 麹菌染色体における大領域重複の作製方法
BR112013008347A2 (pt) 2010-10-06 2016-06-14 Bp Corp North America Inc polipeptídeos de variantes cbh i
CN102206666B (zh) * 2011-04-15 2013-02-06 山东省农业科学院农业资源与环境研究所 一种质粒载体pLGF及其应用
JP6378089B2 (ja) 2011-12-09 2018-08-22 ダニスコ・ユーエス・インク 微生物におけるタンパク質産生のための、Ｂ．ズブチリス（Ｂ．ｓｕｂｔｉｌｉｓ）からのリボソームプロモーター
JP6081245B2 (ja) * 2012-04-07 2017-02-15 キッコーマン株式会社 麹菌染色体における任意領域の重複転座の作製方法
CN102839214A (zh) * 2012-09-06 2012-12-26 许汉鹏 筛选真核细胞基因组内特定基因调控相关机制和分子的方法
US9938551B2 (en) 2012-12-12 2018-04-10 Danisco Us Inc Variants of cellobiohydrolases
CN103834681B (zh) * 2013-11-26 2016-01-13 中国计量学院 一种农杆菌介导遗传转化稻曲菌的方法
US9914932B2 (en) 2014-11-13 2018-03-13 Battelle Memorial Institute Agrobacterium-mediated transformation of lipomyces
US20180030456A1 (en) 2015-02-19 2018-02-01 Danisco Us Inc. Enhanced protein expression
US20180148683A1 (en) 2015-07-07 2018-05-31 Danisco Us Inc. Induction of gene expression using a high concentration sugar mixture
WO2017040499A1 (en) 2015-09-02 2017-03-09 Dupont Nutrition Biosciences Aps Glycoside hydolases and their use in preventing and/or treating a pathogenic infection in an animal
EP4095152A3 (en) 2016-03-04 2022-12-28 Danisco US Inc. Engineered ribosomal promoters for protein production in microorganisms
EP3825399A1 (en) 2016-06-30 2021-05-26 Danisco US Inc. Aspartic proteases
CA3037875A1 (en) 2016-09-23 2018-03-29 Dupont Nutrition Biosciences Aps Use of low ph active alpha-1,4/1,6-glycoside hydrolases as a feed additive for ruminants to enhance starch digestion
US10876103B2 (en) 2016-10-04 2020-12-29 Danisco Us Inc Protein production in filamentous fungal cells in the absence of inducing substrates
US20190330577A1 (en) 2016-12-21 2019-10-31 Dupont Nutrition Biosciences Aps Methods of using thermostable serine proteases
US20200071685A1 (en) 2017-03-06 2020-03-05 Dupont Nutrition Biosciences Aps Novel fungal fucosidases and their use in preventing and/or treating a pathogenic infection in an animal
CN110603323A (zh) 2017-03-15 2019-12-20 杜邦营养生物科学有限公司 使用古细菌丝氨酸蛋白酶的方法
EP3583210B1 (en) 2017-03-15 2021-07-07 Danisco US Inc. Trypsin-like serine proteases and uses thereof
EP3596210A1 (en) 2017-03-15 2020-01-22 DuPont Nutrition Biosciences ApS Trypsin-like serine proteases and uses thereof cross-reference to related application
CN112654703A (zh) 2018-07-06 2021-04-13 杜邦营养生物科学有限公司 用于基于谷类的动物饲料的含木聚糖酶饲料添加剂
KR20220097451A (ko) 2019-11-08 2022-07-07 다니스코 유에스 인크. 향상된 단백질 생산성 표현형을 포함하는 진균 균주 및 이의 방법
KR20230004495A (ko) 2020-04-22 2023-01-06 다니스코 유에스 인크. 사상 진균 세포에서의 개선된 단백질 생산을 위한 조성물 및 방법
CN112680365B (zh) * 2021-02-02 2023-01-31 吉林农业大学 一种白僵菌用液体培养基及白僵菌菌剂制备方法
WO2023003683A2 (en) 2021-07-19 2023-01-26 Danisco Us Inc. Compositions and methods for enhanced protein production in fungal cells
WO2023023644A1 (en) 2021-08-20 2023-02-23 Danisco Us Inc. Polynucleotides encoding novel nucleases, compositions thereof and methods thereof for eliminating dna from protein preparations
WO2023102315A1 (en) 2021-12-01 2023-06-08 Danisco Us Inc. Compositions and methods for enhanced protein production in fungal cells
CN114875046B (zh) * 2022-05-30 2023-07-18 福州大学 一种丝状真菌复制子

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0481008B1 (en) 1989-07-07 1997-11-12 Unilever Plc Process for preparing a protein by a fungus transformed by multicopy integration of an expression vector
DK0672149T3 (da) * 1991-12-09 2001-11-05 Unilever Nv Fremgangsmåde til fremstilling/udskilning af et protein med en transformeret skimmelsvamp ved anvendelse af ekspressions/udskilningsregulerende regioner afledt af et Aspergillus-endoxylanase II-gen

Also Published As

Publication number Publication date
AU7428398A (en) 1998-10-30
JP4307563B2 (ja) 2009-08-05
ES2216284T3 (es) 2004-10-16
DE69822142T2 (de) 2005-03-03
EP0973917A1 (en) 2000-01-26
WO1998045455A1 (en) 1998-10-15
CN1151264C (zh) 2004-05-26
MY121328A (en) 2006-01-28
CA2286307A1 (en) 1998-10-15
ID22929A (id) 1999-12-16
JP2001518786A (ja) 2001-10-16
DE69822142D1 (de) 2004-04-08
EP0973917B1 (en) 2004-03-03
US6255115B1 (en) 2001-07-03
CN1259167A (zh) 2000-07-05

Similar Documents

Publication Publication Date Title
BR9807941A (pt) Processos para a produção e para cultivar um bolor transformado, e, bolor transformado
USD967615S1 (en) Shoe
USD865517S1 (en) Cardboard blank
USD934962S1 (en) Ring grip
USD901367S1 (en) Remote controller for ships
USD905559S1 (en) Bottle
USD976866S1 (en) Loudspeaker
US2016434A (en) Paper cup
USD978832S1 (en) Loudspeaker
USD957891S1 (en) Insulating sleeve for a beverage container
USD971393S1 (en) Humidifier
USD918767S1 (en) Jewelry article
USD947769S1 (en) Charger
USD929754S1 (en) Table
USD859008S1 (en) Paper product
USD872143S1 (en) Cutting blade
USD972524S1 (en) Loudspeaker
USD968875S1 (en) Food grinder
USD902605S1 (en) Paper product
USD892716S1 (en) Tire
US6053377A (en) Wooden garment hanger and method for making same
USD991220S1 (en) Loudspeaker
USD932521S1 (en) Pedestal for vacuum pumps
Reymond et al. Production by protoplast fusion of new strains of Penicillium caseicolum for use in the dairy industry
USD1003566S1 (en) Set of food particles

Legal Events

Date Code Title Description
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]
B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO PUBLICADO NA RPI 1937 DE 19/02/2008