IT1251435B - Vettore genetico per la integrazione multipla stabile di sequenze di dna nel genoma dei lieviti kluyveromyces lactis e saccharomyces cerevisiae e plasmidi che lo contengono. - Google Patents
Vettore genetico per la integrazione multipla stabile di sequenze di dna nel genoma dei lieviti kluyveromyces lactis e saccharomyces cerevisiae e plasmidi che lo contengono.Info
- Publication number
- IT1251435B IT1251435B ITMI912349A ITMI912349A IT1251435B IT 1251435 B IT1251435 B IT 1251435B IT MI912349 A ITMI912349 A IT MI912349A IT MI912349 A ITMI912349 A IT MI912349A IT 1251435 B IT1251435 B IT 1251435B
- Authority
- IT
- Italy
- Prior art keywords
- plasmids
- contain
- dna sequences
- gene
- kluyveromyces lactis
- Prior art date
Links
- 241001138401 Kluyveromyces lactis Species 0.000 title abstract 2
- 108091028043 Nucleic acid sequence Proteins 0.000 title abstract 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 title abstract 2
- 230000002068 genetic effect Effects 0.000 title abstract 2
- 230000010354 integration Effects 0.000 title abstract 2
- 239000013612 plasmid Substances 0.000 title abstract 2
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 title 1
- 230000006801 homologous recombination Effects 0.000 abstract 1
- 238000002744 homologous recombination Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Mycology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
E' descritto un vettore genetico per l'integrazione stabile, per ricombinazione omologa, di specifiche sequenze di DNA nel genoma dei lieviti Kluyveromyces lactis e Saccharonmyces cerevisiae che consente di ottenere eventi integrativi multipli in numero elevato e i plasmidi che lo contengono.
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ITMI912349A IT1251435B (it) | 1991-09-04 | 1991-09-04 | Vettore genetico per la integrazione multipla stabile di sequenze di dna nel genoma dei lieviti kluyveromyces lactis e saccharomyces cerevisiae e plasmidi che lo contengono. |
EP92114838A EP0537456A1 (en) | 1991-09-04 | 1992-08-31 | Genetic vector for multiple stable integration of DNA sequences into the genome of the yeasts kluyveromyces lactis and saccharomyces cerevisiae and plasmids containing the same |
CA002077489A CA2077489A1 (en) | 1991-09-04 | 1992-09-03 | Genetic vector for multiple stable integration of dna sequences into the genome of the yeasts kluyveromyces lactis and saccharomyces cerevisiae and plasmids containing the same |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ITMI912349A IT1251435B (it) | 1991-09-04 | 1991-09-04 | Vettore genetico per la integrazione multipla stabile di sequenze di dna nel genoma dei lieviti kluyveromyces lactis e saccharomyces cerevisiae e plasmidi che lo contengono. |
Publications (3)
Publication Number | Publication Date |
---|---|
ITMI912349A0 ITMI912349A0 (it) | 1991-09-04 |
ITMI912349A1 ITMI912349A1 (it) | 1993-03-04 |
IT1251435B true IT1251435B (it) | 1995-05-09 |
Family
ID=11360611
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ITMI912349A IT1251435B (it) | 1991-09-04 | 1991-09-04 | Vettore genetico per la integrazione multipla stabile di sequenze di dna nel genoma dei lieviti kluyveromyces lactis e saccharomyces cerevisiae e plasmidi che lo contengono. |
Country Status (3)
Country | Link |
---|---|
EP (1) | EP0537456A1 (it) |
CA (1) | CA2077489A1 (it) |
IT (1) | IT1251435B (it) |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0852625A2 (en) * | 1995-09-25 | 1998-07-15 | Chiron Corporation | Pichia secretory leader for protein expression |
US6265186B1 (en) | 1997-04-11 | 2001-07-24 | Dsm N.V. | Yeast cells comprising at least two copies of a desired gene integrated into the chromosomal genome at more than one non-ribosomal RNA encoding domain, particularly with Kluyveromyces |
DK0973918T3 (da) * | 1997-04-11 | 2008-09-22 | Dsm Ip Assets Bv | Gærceller, der omfatter mindst to kopier af et önsket gen integreret i det kromosomale genom ved mere end et ikke-ribosomalt RNA-kodende domæne, navnlig med Kluyveromyces |
EP1318200A3 (en) | 2001-12-07 | 2004-04-07 | Daicel Chemical Industries, Ltd. | Methods for producing optically active alcohols |
AU2003301576A1 (en) | 2002-10-22 | 2004-05-13 | Eisai Co., Ltd. | Gene expressed specifically in dopamine-producing neuron precursor cells after termination of division |
JP5030067B2 (ja) | 2006-09-29 | 2012-09-19 | 国立大学法人 奈良先端科学技術大学院大学 | 新規アルカンポリオール脱水素酵素 |
EP2023143A1 (en) | 2007-08-06 | 2009-02-11 | Boehringer Ingelheim Vetmedica Gmbh | Immunogenic streptococcus proteins |
EP2330190A1 (en) | 2008-09-01 | 2011-06-08 | Daicel Chemical Industries, Ltd. | Process for production of optically active amine derivative |
CN102197141A (zh) | 2008-09-01 | 2011-09-21 | 大赛璐化学工业株式会社 | 制备光学活性胺衍生物的方法 |
JP5787360B2 (ja) | 2009-10-30 | 2015-09-30 | 株式会社ダイセル | 1,3−ブタンジオール生産機能を付与された遺伝子組換え微生物及びその利用 |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IT1196484B (it) * | 1986-07-11 | 1988-11-16 | Sclavo Spa | Vettore ad espressione e secrezione in lieviti,utile per la preparazione di proteine eterologhe |
NZ225597A (en) * | 1987-07-28 | 1991-08-27 | Gist Brocades Nv | Use of kluyveromyces in the production of chymosin, tpa, hsa and other proteins |
DE69033633T2 (de) * | 1989-07-07 | 2001-05-03 | Unilever Nv | Verfahren zur Herstellung eines Proteins mittels eines durch Mehrfachkopie-Integrierung eines Expressionsvektors tranformierten Pilzes |
-
1991
- 1991-09-04 IT ITMI912349A patent/IT1251435B/it active IP Right Grant
-
1992
- 1992-08-31 EP EP92114838A patent/EP0537456A1/en not_active Withdrawn
- 1992-09-03 CA CA002077489A patent/CA2077489A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
EP0537456A1 (en) | 1993-04-21 |
ITMI912349A1 (it) | 1993-03-04 |
ITMI912349A0 (it) | 1991-09-04 |
CA2077489A1 (en) | 1993-03-05 |
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