DE69033633T2 - Verfahren zur Herstellung eines Proteins mittels eines durch Mehrfachkopie-Integrierung eines Expressionsvektors tranformierten Pilzes - Google Patents

Verfahren zur Herstellung eines Proteins mittels eines durch Mehrfachkopie-Integrierung eines Expressionsvektors tranformierten Pilzes

Info

Publication number
DE69033633T2
DE69033633T2 DE69033633T DE69033633T DE69033633T2 DE 69033633 T2 DE69033633 T2 DE 69033633T2 DE 69033633 T DE69033633 T DE 69033633T DE 69033633 T DE69033633 T DE 69033633T DE 69033633 T2 DE69033633 T2 DE 69033633T2
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DE
Germany
Prior art keywords
protein
expression vector
integration
production
yeast
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69033633T
Other languages
English (en)
Other versions
DE69033633D1 (de
Inventor
Marco Luigi Frederic Giuseppin
Cornelis Theodorus Verrips
Maria Teresa Santos Lopes
Roelf Johannes Planta
Johannes Maria A Verbakel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Unilever NV
Original Assignee
Unilever NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB898915659A external-priority patent/GB8915659D0/en
Application filed by Unilever NV filed Critical Unilever NV
Publication of DE69033633D1 publication Critical patent/DE69033633D1/de
Application granted granted Critical
Publication of DE69033633T2 publication Critical patent/DE69033633T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2465—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on alpha-galactose-glycoside bonds, e.g. alpha-galactosidase (3.2.1.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
DE69033633T 1989-07-07 1990-07-09 Verfahren zur Herstellung eines Proteins mittels eines durch Mehrfachkopie-Integrierung eines Expressionsvektors tranformierten Pilzes Expired - Lifetime DE69033633T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB898915659A GB8915659D0 (en) 1989-07-07 1989-07-07 Enzymes,their production and use
EP90201007 1990-04-20

Publications (2)

Publication Number Publication Date
DE69033633D1 DE69033633D1 (de) 2000-10-26
DE69033633T2 true DE69033633T2 (de) 2001-05-03

Family

ID=26125849

Family Applications (2)

Application Number Title Priority Date Filing Date
DE69033633T Expired - Lifetime DE69033633T2 (de) 1989-07-07 1990-07-09 Verfahren zur Herstellung eines Proteins mittels eines durch Mehrfachkopie-Integrierung eines Expressionsvektors tranformierten Pilzes
DE69031710T Expired - Lifetime DE69031710T2 (de) 1989-07-07 1990-07-09 Verfahren zur herstellung eines proteins mittels eines durch mehrfachkopieintegrierung eines expressionsvektors transformierten pilzes

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE69031710T Expired - Lifetime DE69031710T2 (de) 1989-07-07 1990-07-09 Verfahren zur herstellung eines proteins mittels eines durch mehrfachkopieintegrierung eines expressionsvektors transformierten pilzes

Country Status (10)

Country Link
US (1) US6090574A (de)
EP (2) EP0778348B1 (de)
JP (1) JP3273609B2 (de)
AT (2) ATE196505T1 (de)
CA (1) CA2063592C (de)
DE (2) DE69033633T2 (de)
DK (2) DK0778348T3 (de)
ES (2) ES2150188T3 (de)
IE (1) IE81016B1 (de)
WO (1) WO1991000920A2 (de)

Families Citing this family (99)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991014781A1 (en) * 1990-03-19 1991-10-03 Henkel Research Corporation METHOD FOR INCREASING THE OMEGA-HYDROXYLASE ACTIVITY IN $i(CANDIDA TROPICALIS)
JP3133774B2 (ja) * 1990-10-04 2001-02-13 日本食品化工株式会社 新規な宿主−ベクター系
ATE140974T1 (de) * 1990-10-04 1996-08-15 Japan Maize Prod Wirt-vektor-system
IT1251435B (it) * 1991-09-04 1995-05-09 Sclavo Spa Vettore genetico per la integrazione multipla stabile di sequenze di dna nel genoma dei lieviti kluyveromyces lactis e saccharomyces cerevisiae e plasmidi che lo contengono.
WO1994013821A1 (en) * 1992-12-11 1994-06-23 Quest International B.V. The use of the kluyveromyces marxianus inulinase gene promoter for protein production
US6936289B2 (en) 1995-06-07 2005-08-30 Danisco A/S Method of improving the properties of a flour dough, a flour dough improving composition and improved food products
CA2265519A1 (en) * 1996-09-24 1998-04-02 Plant Genetic Systems N.V. Dna-constructs comprising intergenic ribosomal dna and methods to produce proteins using these dna-constructs
US7883872B2 (en) 1996-10-10 2011-02-08 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
US6255115B1 (en) 1997-04-07 2001-07-03 Unilever Patent Holdings Bv Agrobacterium mediated transformation of moulds, in particular those belonging to the genus Aspergillus
EP0870835A1 (de) * 1997-04-07 1998-10-14 Unilever N.V. Transformation von Schimmeln durch Agrobacterium, insbesondere von der Gattung Aspergillus
US6265186B1 (en) 1997-04-11 2001-07-24 Dsm N.V. Yeast cells comprising at least two copies of a desired gene integrated into the chromosomal genome at more than one non-ribosomal RNA encoding domain, particularly with Kluyveromyces
DK0979294T3 (en) * 1997-04-11 2015-08-31 Dsm Ip Assets Bv Gene conversion AS A TOOL FOR CONSTRUCTION OF RECOMBINANT INDUSTRIAL filamentous fungi
ES2308805T3 (es) * 1997-04-11 2008-12-01 Dsm Ip Assets B.V. Celulas de levadura que comprenden al menos dos copias de un gen deseado integradas en el genoma cromosomico en mas de un domicio que codifica el arn no ribosomico particularmente con kluyveromyces.
EP1015612B1 (de) * 1997-05-27 2005-01-26 Hanil Synthetic Fiber Co., Ltd. Verfahren zur herstellung von rekombinanten eiweissen durch verwendung von hocheffizienten expressionsvektoren aus saccharomyces cerevisiae
US6228583B1 (en) * 1997-08-04 2001-05-08 Massachusetts Institute Of Technology Assays for compounds which extend life span
CN1284998A (zh) * 1997-12-22 2001-02-21 尤尼利弗公司 多拷贝基因在霉菌中的定点整合方法
PL341760A1 (en) * 1997-12-22 2001-05-07 Dsm Nv Expression cloning in mildew fungi
WO1999055885A2 (en) * 1998-04-28 1999-11-04 Axys Pharmaceuticals, Inc. Novel serine protease capable of selective cleavage of insulin-like growth factor binding protein
AU771539C (en) 1998-10-06 2005-01-13 Dyadic International (Usa), Inc. Transformation system in the field of filamentous fungal hosts
US7220542B2 (en) 2000-07-17 2007-05-22 Van Den Brink Johannes Maarten Expression cloning in filamentous fungi
AU7398100A (en) * 1999-09-13 2001-04-17 Concordia University A multifunctional system for the efficient manipulation of protein expression infilamentous fungi and method using same
DE19963690A1 (de) * 1999-11-23 2001-06-13 Rhein Biotech Proz & Prod Gmbh Vektoren und Verfahren zur Herstellung rekombinanter Proteine in Pilzen
WO2002092757A2 (en) * 2000-11-15 2002-11-21 Kemin Industries, Inc. Enzyme over-producing transgenic microorganisms
ZA200303353B (en) * 2000-12-13 2004-04-30 Unilever Plc Method for the production of a heterologous protein by a fungus.
AU2002226307A1 (en) * 2001-01-25 2002-08-06 Evolva Biotech A/S Artificial chromosomes comprising concatemers of expressible nucleotide sequences
EP1356037B1 (de) 2001-01-25 2011-03-09 Evolva Ltd. Zellbibliothek
AU2002339115B2 (en) 2001-05-18 2007-03-15 Dupont Nutrition Biosciences Aps Method of preparing a dough with an enzyme
ITMI20011728A1 (it) * 2001-08-06 2003-02-06 Consorzio Per Le Ricerche E Lo Vettore per l'integrazione sito-specifica di sequenze di dna eterologhe in lieviti metilotrofi
WO2004072225A2 (en) * 2003-02-12 2004-08-26 Ramot At Tel Aviv University Ltd. Transgenic fungi expressing bcl-2 and methods of using bcl-2 or portions thereof for improving biomass production, survival, longevity, stress resistance and pathogenicity of fungi
CN101818177A (zh) 2004-07-16 2010-09-01 丹尼斯科公司 脂肪分解酶及其在食品工业中的应用
EP2102366A4 (de) 2006-12-10 2010-01-27 Dyadic International Inc Expression und hochdurchsatz-screening komplexer exprimierter dna-bibliotheken bei fadenpilzen
EP2197893B1 (de) 2007-09-07 2013-07-24 Dyadic International, Inc. Neue pilzenzyme
US20110318795A1 (en) 2007-11-20 2011-12-29 Verwaal Rene Dicarboxylic acid production in a filamentous fungus
CN101896606B (zh) 2007-11-20 2016-12-21 帝斯曼知识产权资产管理有限公司 在重组酵母中生产二羧酸
BRPI0819275B8 (pt) 2007-11-20 2022-07-19 Dsm Ip Assets Bv Célula eucariótica recombinante produtora de ácido succínico e processo para a preparação de ácido succínico
EP2116136A1 (de) 2008-05-08 2009-11-11 Nederlandse Organisatie voor toegepast- natuurwetenschappelijk onderzoek TNO Neue Phytasen
US8546645B2 (en) 2008-10-03 2013-10-01 Agrisoma Biosciences Inc. Production of modified fatty acids in plants through rDNA targeted integration of heterologous genes
EP2408907B8 (de) 2009-03-17 2018-04-18 Dutch DNA Biotech B.V. Verwendung von cellulose- und lignocellulose-aktive proteine
US9353387B2 (en) 2009-04-15 2016-05-31 Dsm Ip Assets B.V. Dicarboxylic acid production process
UA108853C2 (uk) 2009-07-10 2015-06-25 Спосіб ферментації галактози
DE102009035671A1 (de) * 2009-07-30 2011-02-03 Dechema Gesellschaft Für Chemische Technik Und Biotechnologie E.V. Verfahren zur Expression von homologen Proteinen und/oder Peptiden in Pilzen der Klasse Dothideomycetes
WO2011131667A1 (en) 2010-04-21 2011-10-27 Dsm Ip Assets B.V. Cell suitable for fermentation of a mixed sugar composition
EP2388331A1 (de) 2010-05-21 2011-11-23 Nederlandse Organisatie voor toegepast -natuurwetenschappelijk onderzoek TNO Konstitutiver Promoter
US9353376B2 (en) 2010-10-13 2016-05-31 Dsm Ip Assets, B.V. Pentose and glucose fermenting yeast cell
EP2699589A2 (de) 2011-04-22 2014-02-26 DSM IP Assets B.V. Hefezelle mit fähigkeit zur umwandlung von zuckern, einschliesslich arabinose und xylose
EP2554668A1 (de) 2011-08-04 2013-02-06 DSM IP Assets B.V. Zelle zur Fermentierung von Pentosezucker
AR087423A1 (es) 2011-08-04 2014-03-19 Dsm Ip Assets Bv Celula capaz de fermentar azucares pentosas
DK2806754T3 (en) 2012-01-23 2019-02-18 Dsm Ip Assets Bv Diterpene PREPARATION
US20130273619A1 (en) 2012-04-16 2013-10-17 Basf Se Process for the Preparation of (3E, 7E)-Homofarnesol
WO2014060377A1 (en) 2012-10-16 2014-04-24 Dsm Ip Assets B.V. Cells with improved pentose conversion
EP2772545A1 (de) 2013-03-01 2014-09-03 Nederlandse Organisatie voor toegepast -natuurwetenschappelijk onderzoek TNO Stärkeaktive Proteine
EP2992105A1 (de) 2013-05-02 2016-03-09 Dutch DNA Biotech B.V. Bahn für neuartige organische säuren
DK3004366T3 (da) 2013-05-31 2019-05-20 Dsm Ip Assets Bv Mikroorganismer til diterpenproduktion
CN108064135A (zh) 2013-07-15 2018-05-22 帝斯曼知识产权资产管理有限公司 二萜的生产
DK3027632T3 (da) 2013-07-31 2019-06-17 Dsm Ip Assets Bv Indvinding af steviolglycosider fra et fermenteringsmedium
WO2016131818A1 (en) 2015-02-16 2016-08-25 Dsm Ip Assets B.V. Process for producing itaconic acid under anaerobic conditions
CA2980090A1 (en) 2015-04-03 2016-10-06 Dsm Ip Assets B.V. Steviol glycosides
WO2016172282A1 (en) 2015-04-21 2016-10-27 Dsm Ip Assets B.V. Microbial production of terpenoids
PL3298133T3 (pl) 2015-05-22 2020-03-31 Dsm Ip Assets B.V. Komórki drożdży zużywające octan
EP3320104B1 (de) 2015-07-10 2021-11-10 DSM IP Assets B.V. Verfahren zur herstellung einer steviolglycosidzusammensetzung
CA2993744A1 (en) 2015-08-13 2017-02-16 Dsm Ip Assets B.V. Steviol glycoside transport
WO2017050652A1 (en) 2015-09-25 2017-03-30 Dsm Ip Assets B.V. Asparaginase
US11136600B2 (en) 2015-10-06 2021-10-05 Dsm Ip Assets B.V. Eukaryotic cell with increased production of fermentation product
EP3435771A1 (de) 2016-03-31 2019-02-06 DSM IP Assets B.V. Enzymzusammensetzung und herstellung eines milchprodukts mit verbesserten eigenschaften
EP3435772A1 (de) 2016-03-31 2019-02-06 DSM IP Assets B.V. Enzymzusammensetzung und herstellung eines milchprodukts mit verbesserten eigenschaften
WO2017167847A1 (en) 2016-03-31 2017-10-05 Dsm Ip Assets B.V. Production of milk with low lactose content
US10689670B2 (en) 2016-06-14 2020-06-23 Dsm Ip Assets B.V. Recombinant yeast cell
US10787649B2 (en) 2016-07-13 2020-09-29 Dsm Ip Assets B.V. Malate dehyrogenases
BR112019002417B1 (pt) 2016-08-09 2023-05-02 Dsm Ip Assets B.V. Cristalização de glicosídeos de esteviol
WO2018029274A1 (en) 2016-08-09 2018-02-15 Dsm Ip Assets B.V. Crystallization of steviol glycosides
CN110036114B (zh) 2016-08-26 2023-09-29 勒萨弗尔公司 提高的衣康酸生产
WO2018073107A1 (en) 2016-10-19 2018-04-26 Dsm Ip Assets B.V. Eukaryotic cell comprising xylose isomerase
WO2018114912A1 (en) 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
CA3046153A1 (en) 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
WO2018114938A1 (en) 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
EP3559222A1 (de) 2016-12-21 2019-10-30 DSM IP Assets B.V. Varianten lipolytischer enzyme
WO2018114995A1 (en) 2016-12-22 2018-06-28 Dsm Ip Assets B.V. Fermentation process for producing steviol glycosides
US11667886B2 (en) 2017-06-13 2023-06-06 Dsm Ip Assets B.V. Recombinant yeast cell
JP2020530271A (ja) * 2017-06-30 2020-10-22 ピーティーティー グローバル ケミカル パブリック カンパニー リミテッド 安定化されたコピー数の機能性ｄｎａ配列を有する微生物及び関連方法
US11414683B2 (en) 2017-09-26 2022-08-16 Dsm Ip Assets B.V. Acetic acid consuming strain
EP3533878A1 (de) 2018-02-28 2019-09-04 Dutch DNA Biotech B.V. Verfahren zur herstellung von 2-hydroxy-2-methylbutandisäure unter verwendung von aspergillus
BR112020025589A2 (pt) 2018-06-19 2021-03-23 Dsm Ip Assets B.V. variantes de enzima lipolítica
CN111254127B (zh) * 2018-11-30 2022-06-10 康码（上海）生物科技有限公司 蛋白激酶a催化亚基tpk2基因改造方法及其应用
CN111254128A (zh) * 2018-11-30 2020-06-09 康码（上海）生物科技有限公司 蛋白激酶a催化亚基tpk1基因改造方法及其应用
AU2021367159A1 (en) 2020-10-22 2023-05-04 Cargill, Incorporated Microorganisms for diterpene production
FR3122436A1 (fr) * 2021-04-30 2022-11-04 IFP Energies Nouvelles Insertion multicopies d’un gène d’intérêt dans le génome d’un champignon
CA3225417A1 (en) 2021-06-24 2022-12-29 Fonterra Co-Operative Group Limited Recombinant proteins
EP4370690A1 (de) 2021-07-12 2024-05-22 Danisco Us Inc Rekombinante hefezelle
WO2023285279A1 (en) 2021-07-12 2023-01-19 Dsm Ip Assets B.V. Recombinant yeast cell
EP4370692A1 (de) 2021-07-12 2024-05-22 Danisco Us Inc Rekombinante hefezelle
WO2023285297A1 (en) 2021-07-12 2023-01-19 Dsm Ip Assets B.V. Recombinant yeast cell
CN117940570A (zh) 2021-07-12 2024-04-26 丹尼斯科美国公司 重组酵母细胞
EP4370689A1 (de) 2021-07-12 2024-05-22 Danisco Us Inc Rekombinante hefezelle
CN113564206B (zh) * 2021-07-29 2023-09-26 山东大学 一种目的基因多拷贝整合到酿酒酵母染色体rDNA的方法
WO2023079048A1 (en) 2021-11-04 2023-05-11 Dsm Ip Assets B.V. Process for the production of ethanol and recombinant yeast cell
WO2023079049A1 (en) 2021-11-04 2023-05-11 Dsm Ip Assets B.V. Variant polypeptide and recombinant yeast cell
WO2023079050A1 (en) 2021-11-04 2023-05-11 Dsm Ip Assets B.V. Recombinant yeast cell
AU2022388727A1 (en) 2021-11-09 2024-05-16 Amgen Inc. Production of therapeutic proteins
WO2023222614A1 (en) 2022-05-16 2023-11-23 Dsm Ip Assets B.V. Lipolytic enzyme variants

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU600885B2 (en) * 1984-05-25 1990-08-30 Zymogenetics Inc. Stable DNA constructs
GB8415186D0 (en) * 1984-06-14 1984-07-18 Ciba Geigy Ag Polypeptides
EP0173378B1 (de) * 1984-07-27 1991-06-12 Unilever N.V. Verfahren zur Herstellung von einem Polypeptid mittels eines transformierten Mikroorganismus, ein dazu geeigneter transformierter Mikroorganismus, und zur Herstellung dieser Mikroorganismen geeignete DNS Sequenzen
JPS61134325A (ja) * 1984-12-04 1986-06-21 Teijin Ltd ハイブリツド抗体遺伝子の発現方法
JPH0614865B2 (ja) * 1985-12-13 1994-03-02 麒麟麦酒株式会社 α―アセト乳酸脱炭酸酵素をコードするＤＮＡ鎖およびこのＤＮＡ鎖により形質転換された酵母
KR910002604B1 (ko) 1986-05-28 1991-04-27 미쓰비시덴기 가부시기가이샤 영상 재생 장치
CA1339101C (en) * 1986-06-03 1997-07-29 Nicolaas Overbeeke Production of guar alpha-galactosidase and immunologically related alpha-galactosidases by host organisms transformed with recombinant dna methods
GB8629536D0 (en) 1986-12-10 1987-01-21 Unilever Plc Enzymatic detergent composition
PT88556B (pt) * 1987-09-28 1992-11-30 Smithkline Biolog Processo de integracao genomica estavel e de expressao de uma sequencia de cadificacao estranha, em leveduras
DK571587D0 (da) 1987-11-02 1987-11-02 Novo Industri As Enzymatisk detergentsammensaetning
EP0318775B1 (de) * 1987-12-03 1994-02-02 Chisso Corporation Lipasegen
US5001230A (en) * 1988-02-18 1991-03-19 Schering Corporation T cell activation markers
JP3079276B2 (ja) * 1988-02-28 2000-08-21 天野製薬株式会社 組換え体ｄｎａ、それを含むシュードモナス属菌及びそれを用いたリパーゼの製造法
JP2639677B2 (ja) * 1988-03-14 1997-08-13 旭化成工業株式会社 リパーゼの遺伝情報を有するｄｎａ

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Publication number Publication date
DK0778348T3 (da) 2000-12-04
IE81016B1 (en) 1999-09-22
ATE160176T1 (de) 1997-11-15
ES2150188T3 (es) 2000-11-16
DE69031710T2 (de) 1998-03-05
EP0481008A1 (de) 1992-04-22
CA2063592A1 (en) 1991-01-08
WO1991000920A3 (en) 1991-03-07
WO1991000920A2 (en) 1991-01-24
ES2109238T3 (es) 1998-01-16
EP0778348B1 (de) 2000-09-20
DE69033633D1 (de) 2000-10-26
EP0481008B1 (de) 1997-11-12
DE69031710D1 (de) 1997-12-18
ATE196505T1 (de) 2000-10-15
US6090574A (en) 2000-07-18
JP3273609B2 (ja) 2002-04-08
CA2063592C (en) 2003-01-21
EP0778348A1 (de) 1997-06-11
IE902496A1 (en) 1991-02-13
DK0481008T3 (da) 1998-05-04
JPH05501949A (ja) 1993-04-15

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