AR087423A1 - Celula capaz de fermentar azucares pentosas - Google Patents
Celula capaz de fermentar azucares pentosasInfo
- Publication number
- AR087423A1 AR087423A1 ARP120102815A ARP120102815A AR087423A1 AR 087423 A1 AR087423 A1 AR 087423A1 AR P120102815 A ARP120102815 A AR P120102815A AR P120102815 A ARP120102815 A AR P120102815A AR 087423 A1 AR087423 A1 AR 087423A1
- Authority
- AR
- Argentina
- Prior art keywords
- cell
- pentous
- ferment
- sugars
- xylose
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
- C12N9/92—Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P35/00—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
- C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
- C12P5/026—Unsaturated compounds, i.e. alkenes, alkynes or allenes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
- C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/16—Butanols
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
- C12P7/20—Glycerol
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/42—Hydroxy-carboxylic acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/44—Polycarboxylic acids
- C12P7/46—Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/44—Polycarboxylic acids
- C12P7/48—Tricarboxylic acids, e.g. citric acid
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/54—Acetic acid
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/56—Lactic acid
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y503/00—Intramolecular oxidoreductases (5.3)
- C12Y503/01—Intramolecular oxidoreductases (5.3) interconverting aldoses and ketoses (5.3.1)
- C12Y503/01005—Xylose isomerase (5.3.1.5)
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Célula que comprende una secuencia de nucleótidos que codifica una xilosa isomerasa, donde la secuencia de aminoácidos de la xilosa isomerasa presenta una identidad de al menos 75% con la secuencia de aminoácidos que se detalla en SEQ ID Nº 2 y donde la secuencia de nucleótidos es heteróloga con relación al huésped. Una célula de acuerdo con la solicitud puede emplearse en un proceso para obtener un producto de la fermentación, tal como el etanol. Este proceso puede comprender fermentar un medio que contiene una fuente de xilosa con una célula de acuerdo con la presente, de manera tal que la célula fermente la xilosa para generar el producto de la fermentación.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201161515014P | 2011-08-04 | 2011-08-04 | |
EP11176601 | 2011-08-04 |
Publications (1)
Publication Number | Publication Date |
---|---|
AR087423A1 true AR087423A1 (es) | 2014-03-19 |
Family
ID=47628656
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP120102815A AR087423A1 (es) | 2011-08-04 | 2012-08-01 | Celula capaz de fermentar azucares pentosas |
Country Status (16)
Country | Link |
---|---|
US (2) | US9701972B2 (es) |
EP (1) | EP2739729B1 (es) |
JP (1) | JP6048980B2 (es) |
KR (1) | KR20140042888A (es) |
CN (1) | CN103717733A (es) |
AR (1) | AR087423A1 (es) |
AU (1) | AU2012292023A1 (es) |
CA (1) | CA2843064A1 (es) |
DK (1) | DK2739729T3 (es) |
EA (1) | EA201400192A1 (es) |
ES (1) | ES2645963T3 (es) |
IN (1) | IN2014DN00094A (es) |
MX (1) | MX2014001396A (es) |
MY (1) | MY185050A (es) |
PL (1) | PL2739729T3 (es) |
WO (1) | WO2013017644A1 (es) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US10604773B2 (en) * | 2012-10-16 | 2020-03-31 | Dsm Ip Assets B.V. | Cells with improved pentose conversion |
CN107849514A (zh) | 2015-07-13 | 2018-03-27 | 玛拉可再生能源公司 | 增强木糖的微藻代谢 |
AU2016301365A1 (en) | 2015-08-05 | 2018-03-08 | Cargill, Incorporated | Xylose isomerase-modified yeast strains and methods for bioproduct production |
US11692187B2 (en) | 2016-12-21 | 2023-07-04 | Vib Vzw | Xylose isomerases that confer efficient xylose fermentation capability to yeast |
FR3062134B1 (fr) * | 2017-01-24 | 2023-07-21 | Lesaffre & Cie | Obtention de souches de levure performantes pour la metabolisation de l'arabinose |
JP2019068788A (ja) * | 2017-10-11 | 2019-05-09 | トヨタ自動車株式会社 | 組換え酵母及びこれを用いたエタノールの製造方法 |
US20210017526A1 (en) * | 2018-03-27 | 2021-01-21 | Basf Se | Xylose metabolizing yeast |
CN114317515B (zh) * | 2021-12-31 | 2024-04-09 | 内蒙古金达威药业有限公司 | 一种以改性硅藻土为载体的固定化全细胞催化制备β-烟酰胺单核苷酸的方法 |
Family Cites Families (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1990014423A1 (en) | 1989-05-18 | 1990-11-29 | The Infergene Company | Microorganism transformation |
JP3273609B2 (ja) | 1989-07-07 | 2002-04-08 | ユニリーバー・ナームローゼ・ベンノートシヤープ | 発現ベクターのマルチコピー組込みにより形質転換した真菌による蛋白質の製法 |
FR2679920A1 (fr) | 1991-08-02 | 1993-02-05 | Rhone Poulenc Rorer Sa | Levures recombinantes hautement stables pour la production de proteines recombinantes, leur preparation et leur utilisation. |
ES2198410T3 (es) | 1993-07-23 | 2004-02-01 | Dsm Ip Assets B.V. | Cepas recombinantes de hongos filamentosos libres de gen marcador de seleccion: un metodo para obtener dichas cepas y su utilizacion. |
PL336345A1 (en) | 1997-04-11 | 2000-06-19 | Dsm Nv | Genic conversion as a tool for constructing recombined filiform fungi |
US6265186B1 (en) | 1997-04-11 | 2001-07-24 | Dsm N.V. | Yeast cells comprising at least two copies of a desired gene integrated into the chromosomal genome at more than one non-ribosomal RNA encoding domain, particularly with Kluyveromyces |
ID27054A (id) | 1998-05-19 | 2001-02-22 | Dsm Nv | Perbaikan produksi sepalosporin di dalam organisme yang hidup |
EP1141372A2 (en) | 1998-12-22 | 2001-10-10 | Dsm N.V. | Improved in vivo production of cephalosporins |
SE0202090D0 (sv) | 2002-05-08 | 2002-07-04 | Forskarpatent I Syd Ab | A modifierd yeast consuming L-arabinose |
DK2410048T3 (en) | 2005-01-24 | 2016-11-28 | Dsm Ip Assets Bv | A process for the preparation of a compound of interest in a filamentous fungal cell |
EA015925B1 (ru) | 2006-06-29 | 2011-12-30 | ДСМ АйПи АССЕТС Б.В. | Способ получения полипептидов |
MX2010009465A (es) * | 2008-03-07 | 2010-09-22 | Dsm Ip Assets Bv | Una celula fermentadora de azucar pentosa. |
DK2367928T3 (en) * | 2008-12-24 | 2018-06-14 | Dsm Ip Assets Bv | XYLOSE ISOMERAS SIGNS AND THEIR USE IN THE FERMENTATION OF PENTOSET SUGAR |
-
2012
- 2012-08-01 AR ARP120102815A patent/AR087423A1/es unknown
- 2012-08-02 PL PL12741015T patent/PL2739729T3/pl unknown
- 2012-08-02 AU AU2012292023A patent/AU2012292023A1/en not_active Abandoned
- 2012-08-02 WO PCT/EP2012/065088 patent/WO2013017644A1/en active Application Filing
- 2012-08-02 EA EA201400192A patent/EA201400192A1/ru unknown
- 2012-08-02 CN CN201280037246.5A patent/CN103717733A/zh active Pending
- 2012-08-02 KR KR1020147002785A patent/KR20140042888A/ko not_active Application Discontinuation
- 2012-08-02 DK DK12741015.7T patent/DK2739729T3/da active
- 2012-08-02 MX MX2014001396A patent/MX2014001396A/es unknown
- 2012-08-02 ES ES12741015.7T patent/ES2645963T3/es active Active
- 2012-08-02 EP EP12741015.7A patent/EP2739729B1/en active Active
- 2012-08-02 CA CA2843064A patent/CA2843064A1/en not_active Abandoned
- 2012-08-02 US US14/236,191 patent/US9701972B2/en active Active
- 2012-08-02 IN IN94DEN2014 patent/IN2014DN00094A/en unknown
- 2012-08-02 JP JP2014523327A patent/JP6048980B2/ja not_active Expired - Fee Related
- 2012-08-02 MY MYPI2014700064A patent/MY185050A/en unknown
-
2017
- 2017-06-02 US US15/612,728 patent/US10100320B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
MX2014001396A (es) | 2014-05-27 |
IN2014DN00094A (es) | 2015-05-15 |
US9701972B2 (en) | 2017-07-11 |
KR20140042888A (ko) | 2014-04-07 |
JP6048980B2 (ja) | 2016-12-21 |
EA201400192A1 (ru) | 2014-06-30 |
AU2012292023A1 (en) | 2014-01-23 |
MY185050A (en) | 2021-04-30 |
DK2739729T3 (da) | 2017-11-13 |
EP2739729B1 (en) | 2017-08-02 |
ES2645963T3 (es) | 2017-12-11 |
US10100320B2 (en) | 2018-10-16 |
CA2843064A1 (en) | 2013-02-07 |
JP2014521341A (ja) | 2014-08-28 |
WO2013017644A1 (en) | 2013-02-07 |
US20170268012A1 (en) | 2017-09-21 |
US20140162312A1 (en) | 2014-06-12 |
EP2739729A1 (en) | 2014-06-11 |
PL2739729T3 (pl) | 2018-01-31 |
CN103717733A (zh) | 2014-04-09 |
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