BRPI0921966A8 - Célula hospedeira de levedura termotolerante, método de fermentar celulose e cultura - Google Patents
Célula hospedeira de levedura termotolerante, método de fermentar celulose e culturaInfo
- Publication number
- BRPI0921966A8 BRPI0921966A8 BRPI0921966A BRPI0921966A BRPI0921966A8 BR PI0921966 A8 BRPI0921966 A8 BR PI0921966A8 BR PI0921966 A BRPI0921966 A BR PI0921966A BR PI0921966 A BRPI0921966 A BR PI0921966A BR PI0921966 A8 BRPI0921966 A8 BR PI0921966A8
- Authority
- BR
- Brazil
- Prior art keywords
- cellulose
- culture
- cellulases
- host cell
- yeast host
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
- C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2445—Beta-glucosidase (3.2.1.21)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P39/00—Processes involving microorganisms of different genera in the same process, simultaneously
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/14—Multiple stages of fermentation; Multiple types of microorganisms or re-use of microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01021—Beta-glucosidase (3.2.1.21)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
LEVEDURA EXPRESSANDO CELULASES PARA SACARIFICAÇÃO E FERMENTAÇÃO SIMULTÂNEAS USANDO CELULOSE Células hospedeiras compreendendo Kluveryomyces expressando celulases heterólogas produzem etanol a partir de celulose. Além disso, múltiplas células hospedeiras que expressam celulases heterólogas diferentes podem ser cocultivadas e usadas para produzir etanol a partir de celulose. As cepas de levedura e coculturas de cepas de levedura da invenção podem ser usadas para produzir etanol por si só, ou também podem ser usadas em combinação com celulases externamente adicionadas para aumentar a eficácia dos processos de sacarificação e fermentação.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US11698108P | 2008-11-21 | 2008-11-21 | |
US61/116,981 | 2008-11-21 | ||
PCT/US2009/065571 WO2010060056A2 (en) | 2008-11-21 | 2009-11-23 | Yeast expressing cellulases for simultaneous saccharification and fermentation using cellulose |
Publications (3)
Publication Number | Publication Date |
---|---|
BRPI0921966A2 BRPI0921966A2 (pt) | 2017-10-24 |
BRPI0921966A8 true BRPI0921966A8 (pt) | 2017-12-12 |
BRPI0921966B1 BRPI0921966B1 (pt) | 2019-05-07 |
Family
ID=42198846
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BRPI0921966-8A BRPI0921966B1 (pt) | 2008-11-21 | 2009-11-23 | Célula hospedeira de levedura termotolerante, método de fermentar celulose e cultura |
Country Status (10)
Country | Link |
---|---|
US (4) | US9102955B2 (pt) |
EP (1) | EP2361311B1 (pt) |
CN (2) | CN108315272A (pt) |
AU (1) | AU2009316309B2 (pt) |
BR (1) | BRPI0921966B1 (pt) |
CA (3) | CA3092340A1 (pt) |
DK (1) | DK2361311T3 (pt) |
ES (1) | ES2621181T3 (pt) |
NZ (1) | NZ593469A (pt) |
WO (1) | WO2010060056A2 (pt) |
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US9994835B2 (en) | 2008-05-11 | 2018-06-12 | Lallemand Hungary Liquidity Management Llc | Construction of protrophic/celluloytic yeast strains expressing tethered and secreted cellulases |
CA3092340A1 (en) | 2008-11-21 | 2010-05-27 | Universiteit Stellenbosch | Yeast expressing cellulases for simultaneous saccharification and fermentation using cellulose |
BR112012011355A2 (pt) | 2009-10-26 | 2015-09-15 | Universiteit Stellenbosch Also Konown As Stellenbosch University | expressao heterologa de genes de celobiohidrolase 2 fungicos em levedura |
US20130071884A1 (en) | 2009-11-06 | 2013-03-21 | Agrivida, Inc. | Plants expressing cell wall degrading enzymes and expression vectors |
US9605269B2 (en) | 2010-05-05 | 2017-03-28 | Lallemand Hungary Liquidity Management Llc | Detoxification of biomass derived acetate via metabolic conversion to ethanol, acetone, isopropanol, or ethyl acetate |
WO2011153516A2 (en) | 2010-06-03 | 2011-12-08 | Mascoma Corporation | Yeast expressing saccharolytic enzymes for consolidated bioprocessing using starch and cellulose |
US10443068B2 (en) | 2010-06-25 | 2019-10-15 | Agrivida, Inc. | Plants with engineered endogenous genes |
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CN103409333B (zh) * | 2013-05-20 | 2015-01-28 | 山东大学 | 一株持续高效分泌β-葡萄糖苷酶的重组酿酒酵母菌株及其应用 |
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WO2010124000A2 (en) | 2009-04-21 | 2010-10-28 | The Trustees Of Dartmouth College | Modified cipa gene from clostridium thermocellum for enhanced genetic stability |
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DK2361311T3 (en) | 2017-04-24 |
US20120129229A1 (en) | 2012-05-24 |
BRPI0921966A2 (pt) | 2017-10-24 |
US20210388398A1 (en) | 2021-12-16 |
US9988652B2 (en) | 2018-06-05 |
NZ593469A (en) | 2012-12-21 |
EP2361311B1 (en) | 2017-01-18 |
AU2009316309A1 (en) | 2011-10-20 |
EP2361311A4 (en) | 2013-03-27 |
WO2010060056A3 (en) | 2010-07-15 |
ES2621181T3 (es) | 2017-07-03 |
US9102955B2 (en) | 2015-08-11 |
AU2009316309B2 (en) | 2016-07-07 |
CA2744495C (en) | 2017-05-30 |
US20160010117A1 (en) | 2016-01-14 |
CA3092340A1 (en) | 2010-05-27 |
CN108315272A (zh) | 2018-07-24 |
CA2964245C (en) | 2020-10-27 |
EP2361311A2 (en) | 2011-08-31 |
US20180258449A1 (en) | 2018-09-13 |
CA2744495A1 (en) | 2010-05-27 |
WO2010060056A2 (en) | 2010-05-27 |
CN102272303A (zh) | 2011-12-07 |
CA2964245A1 (en) | 2010-05-27 |
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