BR112013003906A2 - uso de proteínas da família glicosideo hidrolase no processamento de celulose. - Google Patents

uso de proteínas da família glicosideo hidrolase no processamento de celulose.

Info

Publication number
BR112013003906A2
BR112013003906A2 BR112013003906A BR112013003906A BR112013003906A2 BR 112013003906 A2 BR112013003906 A2 BR 112013003906A2 BR 112013003906 A BR112013003906 A BR 112013003906A BR 112013003906 A BR112013003906 A BR 112013003906A BR 112013003906 A2 BR112013003906 A2 BR 112013003906A2
Authority
BR
Brazil
Prior art keywords
proteins
glycoside hydrolase
hydrolase family
cellulose
family proteins
Prior art date
Application number
BR112013003906A
Other languages
English (en)
Inventor
Baidyaroy Dipnath
Torok Janos
Rao Kripa
Szabo Lorand
Clark Louis
Campoiano Onorato
Original Assignee
Codexis Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Codexis Inc filed Critical Codexis Inc
Publication of BR112013003906A2 publication Critical patent/BR112013003906A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C12N9/242—Fungal source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/14—Multiple stages of fermentation; Multiple types of microorganisms or re-use of microorganisms
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

uso de proteínas da família glicosideo hidrolase no processamento de celulose. a presente invenção fornece proteínas gh61 recombinantes obtidas a partir de myceliophtora thermophila e ácidos nucleicos que codificam tais proteínas. a presente invenção fornece também frações de proteínas isoladas do sobrenadante de m. thermophila com atividade de proteínas gh61. esses preparados podem ser utilizados para aumentar o rendimento de produtos de reações nas quais um substrato contendo celulose é submetido à sacarificação por uma ou mais enzimas celulase, tais cono endoglucanase, <225>-glicosidase ou celobiohidrolase. podem ser utilizadas combinações de proteínas de proteína gh61 e celulases para quebrar a biomassa celulósica em açúcares fermentáveis na produção de etanol.
BR112013003906A 2010-08-20 2011-08-22 uso de proteínas da família glicosideo hidrolase no processamento de celulose. BR112013003906A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US37578810P 2010-08-20 2010-08-20
PCT/US2011/048700 WO2012024698A1 (en) 2010-08-20 2011-08-22 Use of glycoside hydrolase 61 family proteins in processing of cellulose

Publications (1)

Publication Number Publication Date
BR112013003906A2 true BR112013003906A2 (pt) 2018-07-17

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
BR112013003906A BR112013003906A2 (pt) 2010-08-20 2011-08-22 uso de proteínas da família glicosideo hidrolase no processamento de celulose.

Country Status (9)

Country Link
US (2) US8298795B2 (pt)
EP (1) EP2606131B1 (pt)
CN (1) CN103080306B (pt)
BR (1) BR112013003906A2 (pt)
CA (1) CA2807702C (pt)
DK (1) DK2606131T3 (pt)
ES (1) ES2623288T3 (pt)
HU (1) HUE033334T2 (pt)
WO (1) WO2012024698A1 (pt)

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WO2013028701A1 (en) 2011-08-22 2013-02-28 Codexis, Inc. Gh61 glycoside hydrolase protein variants and cofactors that enhance gh61 activity
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CN109929855B (zh) * 2017-12-15 2022-12-13 中国科学院大连化学物理研究所 多糖裂解单加氧酶lpmo 9d编码基因及酶与制备和应用
CN108796026A (zh) * 2018-06-29 2018-11-13 齐齐哈尔龙江阜丰生物科技有限公司 赖氨酸的生产、提取以及纯化工艺
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CN116396868B (zh) * 2021-11-23 2023-09-22 北京工商大学 一种发酵花生壳制备高品质膳食纤维的微生物菌剂及其方法
CN114107257B (zh) * 2021-11-24 2023-08-08 广东省科学院微生物研究所(广东省微生物分析检测中心) 一种糖苷水解酶及其应用
CN114107139B (zh) * 2021-12-31 2023-11-17 武汉工程大学 一株烟管菌f21及其在生产纤维素酶中的应用
CN116376707B (zh) * 2022-10-18 2024-06-14 南京灿辰微生物科技有限公司 一种草酸青霉菌0710-26及其应用
CN117343950B (zh) * 2023-12-06 2024-02-06 中国农业科学院北京畜牧兽医研究所 一种提高里氏木霉表达外源蛋白表达水平的方法

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