KR100618495B1 - 섬유상의 진균성 숙주 영역에서의 형질전환 시스템:크리소스포륨속에서 - Google Patents
섬유상의 진균성 숙주 영역에서의 형질전환 시스템:크리소스포륨속에서 Download PDFInfo
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- 0 *CCCC**(CC(C(*)CC(*)C(*)CCOCCO)N=O)C(C1*)(C(*)C1N=C)O Chemical compound *CCCC**(CC(C(*)CC(*)C(*)CCOCCO)N=O)C(C1*)(C(*)C1N=C)O 0.000 description 3
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Abstract
Description
샘플 | > 50% 효소적 활성을 유지하는 pH 범위 | > 70% 효소적 활성을 보유하는 pH 범위 | 최대값에서 안정성 % (20시간,50℃) | ||||
CMC ase | RBB- CMC ase | 기타의 기질 | CMC- ase | RBB- CMD ase | 기타의 기질 | pH 7.5/8 | |
30 Kd 프로테아제(알칼리성) 30 kD | - | - | 12.5 | - | - | 12.0 | - |
Xyl (알칼리성) | - | - | 10.0 | - | - | 8.5 | 80 |
51 kD Xyl | - | - | 8.0 | - | - | 7.5 | - |
60 kD Xyl | - | - | 9.5 | - | - | 9.0 | 58 |
45 kD Xyl | 7.0 | 8.0 | - | 6.5 | 7.0 | - | 75 |
55 kD 엔도 | 8.0 | 8.0 | - | 7.0 | 7.0 | - | 55 |
25 kD(21.8 kD*)엔도 | 7,5 | 10.0 | - | 6.5 | 9.0 | - | 80 |
43 kD(39.6 kD*)엔도 | 8.0 | 8.0 | - | 7.2 | 7.2 | - | - |
45 kD α,β-Gal/β-Gluc | - | - | 6.8 | - | - | 5.7 | - |
β-Glue 흔적이 있는 48 kD CBH | 5.2 | 7.5 | 8.0 | 5.0 | 6.8 | - | - |
55 kD CBH | 8.0 | 9.0 | - | 7.4 | 8.5 | - | 70 |
65 kD PGU | - | - | 8.0 | - | - | 7.3 | - |
90 kD 프로테아제 | - | - | 9.0 | - | - | 9.0 | - |
100 kD 에스테라제 | - | - | 9.0 | - | - | 9.0 | - |
도27: 55 kD CBH (pI 4.4)용 셀로비오스에 의한 MUF-셀로비오시드에 대한 활성 억제: pH 4.5, 40℃.
도28: 아비셀에 대한 25 kD 엔도 (pI 4.1) 및 55 kD CBH (pI 4.4) 사이이 상승 효과(40℃, pH 5, 25 분).
도29: F-60-8 UF-conc. 샘플의 결합된 분절로부터 분리된 효소에 의한 (a) CMC 및 (b) avicel의 완벽한 가수분해 (50℃, pH 5): CMC 및 아비셀의 농도- 5 g/l, 25kD 엔도의 농도 = 0.01 g/l, 43 kD 엔도의 농도 = 0.02 g/l; 1-25 kD 엔도 (pI 4.1), 2-43 kD 엔도 (pI 4.2).
도30: 55 kD CBH (pI 4.4)에 의한 (1) CMC 및 (2) 아비셀의 완벽한 가수분해, (a) 글루코노-δ-락톤 (50℃, pH 4.5) 부재하에서, (b) 글루코노-δ-락톤 (50℃, pH 4.5) 존재하에서: CMC 및 아비셀의 농도 = 5 g/l, 단백질의 농도 = 0.1 g/l, 글루코노-δ-락톤의 농도 = 5 g/l.
도31: F-60-8 UF-conc. 샘플에서 분리된 효소의 CMCase 및 RBB-CMCase 활성의 pH-의존성: 1-25 kD 엔도(pI 4.1), 2-43 kD 엔도(pI 4.2)
도32: 55 kD CBH (pI 4.4)의 CMCase 및 RBB-CMCase 활성의 pH-의존성.
도33: F-60-8 UF-conc. 샘플의 결합된 분절로부터 분리된 효소의 CMCase 활성 (pH 4.5)의 온도 의존성: 1-55 kD CBH(pI 4.4), 2-25 kD 엔도(pI 4.1), 3-43 kD 엔도(pI 4.2)
도34: F-60-8 UF-conc. 샘플의 결합된 분절로부터 분리된 효소의 pH-안정성(50℃): 1-55 kD CBH (pI 4.4), 2-25 kD 엔도 (pI 4.1), 3-43 kD 엔도 (pI 4.2).
도35: F-60-8 Uf-conc. 샘플의 결합된 분절로부터 분리된 효소의 흡수.
Gs (pH 6.8) | Pridham Agar (PA, pH 6.8) | |||||
NG7C-19 | UV 18-25 | T. r. 11D5 | NG7C-19 | UV 18-25 | T. r. 11D5 | |
플레오마이신 | 7.5 ㎍/ml | 10 ㎍/ml | 5-7.5 ㎍/ml | 2.5 ㎍/ml | 10 ㎍/ml | 2.5 ㎍/ml |
하이그로마이신 | 7.5-10 ㎍/ml | 10 ㎍/ml | 10 ㎍/ml | 15 ㎍/ml | 25㎍/ml | 15 ㎍/ml |
T. 리세이 | NG7C-19 | UV18-25 | |
유효성 | 106/200 ㎕ | 5 106/200 ㎕ | 5 106/200 ㎕ |
200㎕ 당 형질전환체 | 2500 | 104 | 104 |
106 의 생육가능한 셀 당 형질전환체 | 2500 | 2000 | 2000 |
벡터 | 균주 | 형질전환 | 형질전환이 없음. | 배양액에서 시험 |
PUT1150 | UV 18-24 | 플레오마이신 선택 | 285 | 5 |
T.geodes | 플레오마이신 선택 | 144 | 5 | |
PUT1152 | UV 18-24 | 형질전환 pAN8.1 | 398 | 5 |
T.geodes | 형질전환 pAN8.1 | 45 | 4 | |
PF6g | UV 18-24 | 형질전환 pAN8.1 | 252 | 6 |
T.geodes | 형질전환 pAN8.1 | 127 | 5 | |
PUT1162 | UV 18-24 | 플레오마이신 선택 | >400 | |
T.geodes | 아직 미실시 |
Western 블롯에서 추정된 Sh ble 량 | 생산 배지내에서 추정된 Sh ble 농도 | |
미형질전환된 NG7C-19 | 검출 불가 | |
Ng7C-19::720 클론 4-1 | 25 ng | 0.25 mg/l |
Ng7C-19::720 클론 5-1 | 25 ng | 0.25 mg/l |
Ng7C-19::720 클론 2-2 | 250 ng | 2.5 mg/l |
미형질전환된 NG7C-19 | 검출 불가 | |
UV18-25::720 클론 1-2 | 500 ng | 5 mg/l |
UV18-25::720 클론 3-1 | 250 ng | 2.5 mg/l |
배양배지에서 활성 HLZ 농도 | |
미형질전환된 NG7C-19 | 0 mg/l |
Ng7C-19::970G 클론 4 | 4 mg/l |
Ng7C-19::970G 클론 5 | 11 mg/l |
미형질전환된 UV 18-25 | 0 mg/l |
UV18-25::970 클론 1 | 8 mg/l |
UV18-25::970 클론 2 | 4 mg/l |
UV18-25::970 클론 3 | 2 mg/l |
UV18-25::970 클론 2 | 2.5 mg/l |
배양배지에서 활성 크실라나제 II | 배양배지에서 크실라나제 II 특정 활성 | |
미형질전환된 UV 18-25 | 3.9 U./ml | 3.8 U./mg 총 단백질 |
UV18-25::1064 클론 7-1 | 4.7 U./ml | 4.7 U./mg 총 단백질 |
UV18-25::1064 크론 7-2 | 4.4 U./ml | 4.3 U./mg 총 단백질 |
UV18-25::1065 클론 1-1 | 29.7 U./ml | 25.6 U./mg 총 단백질 |
UV18-25::1065 클론 1-2 | 30.8 U./ml | 39.4 U./mg 총 단백질 |
배양물 | 총 단백질 | CMCase | β-글루카나제 | pH 값 | ||
mg/ml | u/ml | u/mg | u/ml | u/mg | ||
*UV 18-25 | 100% | 100% | 100% | 100% | 100% | 7.90 |
1150-23 | 94% | 105% | 111% | 140% | 149% | 7.90 |
-30 | 96% | 105% | 110% | 145% | 151% | 8.10 |
1152-3 | 94% | 112% | 120% | 147% | 156% | 7.85 |
-4 | 100% | 105% | 105% | 132% | 132% | 7.90 |
1160-2 | 69% | 81% | 118% | 90% | 131% | 7.90 |
-4 | 73% | 72% | 98% | 83% | 114% | 8.35 |
-1 | 92% | 95% | 103% | 120% | 130% | 8.45 |
1162-1 | 102% | 105% | 103% | 145% | 142% | 8.20 |
-11 | 112% | 109% | 98% | 115% | 103% | 8.20 |
F6g-20 | 104% | 102% | 98% | 130% | 125% | 7.90 |
-25 | - | - | - | - | - | - |
Claims (29)
- 시험관내 돌연변이 유발법 또는 재조합 방법에 의하여 수득되는 돌연변이체 크리소스포륨(Chrysosporium) 균주로서, 상기 균주는 관심있는 폴리펩티드를 암호화하는 핵산 서열을 포함하며, 상기 핵산 서열은 작동적으로 발현-조절 영역에 연결되어 있고 선택적으로 분비 시그널 서열에 연결되어 있으며, 동일한 조건에서 상기 돌연변이체 균주가 상기 균주에 상응하는 비-돌연변이체 균주보다도 고농도로 상기 폴리펩티드를 발현시키는 돌연변이체 크리소스포륨 균주.
- 제1항에 있어서, 상기 돌연변이체가 이종 폴리펩티드-암호화 핵산 서열에서 선택된 1 이상의 이종 핵산 서열의 안정한 도입을 포함하는 재조합 방법에 의하여 수득되는 것인 돌연변이체 크리소스포륨 균주.
- 제2항에 있어서, 상기 폴리펩티드가 식물, 동물(인간 포함), 조류(藻類), 세균, 고세균 또는 진균 기원의 이종 폴리펩티드인 돌연변이체 크리소스포륨 균주.
- 제1항 또는 제2항에 있어서, 상기 폴리펩티드가 동종의 폴리펩티드로서, 동일한 조건하에서 상응하는 비-돌연변이 균주에서 보다 고농도로 발현되는 것인 돌연변이체 크리소스포륨 균주.
- 제1항 또는 제2항에 있어서, 상기 폴리펩티드가 탄수화물-분해 효소, 프로테아제, 리파제, 에스테라제, 기타의 하이드롤라제, 옥시도리덕타제 및 트랜스퍼라제로부터 선택되는 것인 돌연변이체 크리소스포륨 균주.
- 제1항 또는 제2항에 있어서, 상기 폴리펩티드가 유기산을 비롯한 제1 대사 산물과 항생제를 비롯한 제2 대사산물을 (과)생산하게 하는 진균성 효소로부터 선택되는 돌연변이체 크리소스포륨 균주.
- 제1항 또는 제2항에 있어서, 상기 폴리펩티드가 6 이상의 pH에서 최적의 활성 및/또는 안정성을 나타내거나, 및/또는 6 이상의 pH에서 활성 및/또는 안정성의 70 % 이상을 나타내는 것인 돌연변이체 크리소스포륨 균주.
- 제1항 또는 제2항에 있어서, 상기 돌연변이체 크리소스포륨 균주가 이종의 시그널 서열을 포함하는 것인 돌연변이체 크리소스포륨 균주.
- 제8항에 있어서, 상기 돌연변이체 크리소스포륨 균주가 진균성, 예컨대 자낭균(ascomycete)의 시그널 서열을 포함하는 것인 돌연변이체 크리소스포륨 균주.
- 제9항에 있어서, 진균성 시그널 서열이 셀룰라제, β-갈락토시다제, 크실라나제, 펙티나제, 에스테라제, 프로테아제, 아밀라제, 폴리갈락투로나제 또는 하이 드로포빈의 시그널 서열인 돌연변이체 크리소스포륨 균주.
- 제1항 또는 제2항에 있어서, 상기 돌연변이 크리소스포륨 균주가 약물에 대해 내성을 공여하거나 또는 영양 부족을 경감시키는 마아커와 같은 선택적 마아커를 더 포함하는 것인 돌연변이체 크리소스포륨 균주.
- 제1항 또는 제2항에 있어서, 상기 돌연변이체 크리소스포륨 균주가 이종의 발현-조절 영역, 바람직하게는 진균성 발현-조절 서열을 포함하는 것인 돌연변이체 크리소스포륨 균주.
- 제12항에 있어서, 상기 발현-조절 영역이 유도 프로모터를 포함하는 것인, 돌연변이체 크리소스포륨 균주.
- 제12항에 있어서, 상기 발현-조절 영역이 진균성 셀로비오하이드롤라제, 글루코-아밀라제, 글리세르알데히드 포스페이트 데하이드로제나제, 알콜 데하이드로제나제 A, 알콜 데하이드로제나제 R, 포스포글리세레이트, 아스파르틱 프로테이나제, 리파제, 베타-갈락토시다제, 하이드로포빈, 프로테아제, 아밀라제, 크실라나제, 펙티나제, 에스테라제, 엔도-글로카나제 또는 폴리갈라투로나제 프로모터를 포함하는 것인 돌연변이체 크리소스포륨 균주.
- 제1항에 있어서, 상기 돌연변이체가 자외선 조사 및 화학적 돌연변이 유발 중 1 이상, 바람직하게는 제1차 자외선 조사 단계, N-메틸-N'-니트로-N-니트로소구아니딘 처리 단계 및 제2차 자외선 조사 단계를 포함하는 돌연변이 유발 단계에 의하여 수득되는 것인, 돌연변이체 크리소스포륨 균주.
- 제1항 또는 제2항에 있어서, 상기 돌연변이체가 크리소스포륨 루크노웬스(Chrysosporium lucknowense) , 특히 C. 루크노웬스 균주 C1(VKM F-3500 D)로부터 유래되는 것인, 돌연변이체 크리소스포륨 균주.
- 제16항에 있어서, 상기 돌연변이체가 크리소스포륨 루크노웬스 돌연변이체 균주 UV13-6(NKM F-3532 D), NG7C-19(VKM F-3633 D) 및 UV18-25(VKM F-3631 D) 중 하나에 상응하거나 이로부터 유래되는 것인, 돌연변이체 크리소스포륨 균주.
- 제1항 또는 제2항에 있어서, 상기 균주가 트리코데르마 리세이(Trichoderma reesai) 생물체량(biomass)의 절반 이하의 생물체량을 나타내며, 배양물 내의 상기 트리코데르마는 같은 최적 조건에서 배양되면 200 ∼ 600 cP의 점성도를 나타내는 것인, 돌연변이체 크리소스포륨 균주.
- 제1항 또는 제2항에 있어서, 상기 균주가 크리소스포륨 루크노웬스 돌연변이체 균주 C1, (VKM F-3500 D), UV13-6(NKM F-3532 D), NG7C-19(VKM F-3633 D) 및 UV18-25(VKM F-3631 D) 중 임의의 것에 의하여 생산된 셀룰라제 함량(몰/리터) 이상을 생산하는 것인 돌연변이체 크리소스포륨 균주.
- 제1항 또는 제2항에 있어서, 상기 균주가 크리소스포륨 루크노웬스 균주 C1 (VKM F-3500 D)에 의하여 생산된 프로테아제 보다 적은 양을 생산하는 것인, 바람직하게는 상기 C1 균주에 의하여 생산된 양의 반 이하를 생산하는 것인 돌연변이체 크리소스포륨 균주.
- 시험관내 돌연변이 유발법 또는 재조합 방법에 의하여 수득되는 돌연변이체 크리소스포륨 균주, 바람직하게는 C. 루크노웬스 C1 (VKM F-3500 D) 또는 UV18-25(VKM F-3631 D)로부터 유래한 핵산 발현-조절 영역을 포함하며, 이러한 영역이 작동적으로 폴리펩티드-암호화 핵산 서열에 연결되어 있는 핵산 구조체 (construct).
- 제21항에 있어서, 상기 발현-조절 영역이 셀룰라제 발현 또는 크실라나제 발현과 관련된 프로모터 서열, 바람직하게는 셀로비오하이드롤라제(CBH1) 프로모터 서열을 포함하는 것인 핵산 구조체.
- 제21항 또는 제22항에 따른 핵산 구조체를 함유하며, 코딩 핵산 서열에 의하여 암호화되는 폴리펩티드를 발현시킬 수 있는 재조합 미생물 균주, 바람직하게는 진균성 균주,
- 단백질 또는 폴리펩티드를 발현하게 하고 바람직하게는 분비하게 하는 조건하에서 제1항 또는 제2항의 균주를 배양하는 단계 및 생산된 폴리펩티드를 회수하는 단계를 포함하는 폴리펩티드 생산 방법.
- 제24항에 있어서, 상기 방법이 상기 폴리펩티드의 전구체를 폴피펩티드 또는 관심있는 전구체로의 절단 단계를 더 포함하는 방법으로서, 바람직하게는 Kex-2 유사 프로테아제, 임의의 염기성 아미노산 짝을 이룬 프로테아제 또는 Kex-2로 절단하는 것인 방법.
- 제24항에 있어서, pH 6∼9, 및/또는 25 ℃ 내지 43 ℃ 사이의 온도에서 배양시키는 방법.
- 이종 또는 동종의 폴리펩티드를 암호화하는 핵산 서열을 크리소스포륨 균주에 안정적으로 도입하는 단계를 포함하는, 제1항 또는 제2항의 돌연변이체 크리소스포륨 균주를 생산하는 방법으로서, 상기 핵산 서열은 발현 조절 영역에 작동적으로 연결되고, 상기 도입은 섬유상의 진균을 형질전환시키기 위해 그 자체가 공지된 방식으로 실시되는 것인 방법.
- 제27항에 있어서, 상기 형질전환 방법이 원형질체(protoplast) 형질전환 방법인 방법.
- SDS PAGE에 의하여 약 30 kD의 분자량, pI 9.1을 가지며, 서열 번호 5번에 나타낸 아미노산 서열을 갖는 120 아미노산의 스트레치와 75 % 이상의 아미노산 동일성을 갖는 것인 크실라나제 F 군의 크리소스포륨 크실라나제.
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Families Citing this family (98)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7883872B2 (en) | 1996-10-10 | 2011-02-08 | Dyadic International (Usa), Inc. | Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose |
EP1117808B1 (en) | 1998-10-06 | 2004-12-29 | Mark Aaron Emalfarb | Transformation system in the field of filamentous fungal hosts: in chrysosporium |
DK1123974T3 (da) | 1998-10-23 | 2010-08-23 | Meiji Seika Kaisha | Endoglucanaser og cellulasefremstillinger indeholdende dem |
KR100312070B1 (ko) * | 1999-03-15 | 2001-11-03 | 박호군 | 고속 선별법을 포함하는 개선된 형질을 갖는 효소 변이체의 제조방법 |
KR20020026456A (ko) | 2000-04-13 | 2002-04-10 | 에말파브 마크 아론 | 사상균에서 발현된 dna 라이브러리의 고산출량 스크리닝 |
DK1276876T3 (da) * | 2000-04-13 | 2007-07-16 | Mark Aaron Emalfarb | Ekspressionsregulerende sekvenser fra den filamentöse svamp Chrysosporium |
JP4230149B2 (ja) * | 2000-05-22 | 2009-02-25 | 明治製菓株式会社 | エンドグルカナーゼ酵素nce5及びそれを含んでなるセルラーゼ調製物 |
EP2305311A3 (en) | 2001-10-10 | 2011-07-20 | BioGeneriX AG | Glycoconjugation of peptides |
PT2279753E (pt) | 2001-10-10 | 2016-01-15 | Novo Nordisk As | Remodelação e glicoconjugação de péptidos |
CA2522345A1 (en) | 2003-04-09 | 2004-11-18 | Neose Technologies, Inc. | Glycopegylation methods and proteins/peptides produced by the methods |
FI118339B (fi) * | 2004-09-21 | 2007-10-15 | Ab Enzymes Oy | Uusi lakkaasientsyymi ja sen käyttö |
CA2592550C (en) * | 2004-12-30 | 2015-05-19 | Genencor International, Inc. | Novel variant hypocrea jecorina cbh2 cellulases |
US7256032B2 (en) | 2005-12-22 | 2007-08-14 | Ab Enzymes Oy | Enzymes |
FI119325B (fi) * | 2005-12-22 | 2008-10-15 | Ab Enzymes Oy | Uusia endoglukanaasi polypeptidejä ja niiden tuotto ja käyttö |
DK1969123T3 (en) * | 2005-12-22 | 2017-08-28 | Ab Enzymes Oy | Hitherto UNKNOWN ENZYMS |
ATE513907T1 (de) | 2006-03-20 | 2011-07-15 | Novozymes Inc | Polypeptide mit endoglucanase-aktivität und polynukleotide zu ihrer kodierung |
WO2007123801A1 (en) * | 2006-04-05 | 2007-11-01 | Genencor International, Inc. | Filamentous fungi having reduced udp-galactofuranose content |
WO2008008793A2 (en) * | 2006-07-10 | 2008-01-17 | Dyadic International Inc. | Methods and compositions for degradation of lignocellulosic material |
US20080026376A1 (en) | 2006-07-11 | 2008-01-31 | Huaming Wang | KEX2 cleavage regions of recombinant fusion proteins |
WO2008073914A2 (en) | 2006-12-10 | 2008-06-19 | Dyadic International Inc. | Expression and high-throughput screening of complex expressed dna libraries in filamentous fungi |
US9862956B2 (en) | 2006-12-10 | 2018-01-09 | Danisco Us Inc. | Expression and high-throughput screening of complex expressed DNA libraries in filamentous fungi |
CN101855345A (zh) | 2007-05-10 | 2010-10-06 | 诺维信股份有限公司 | 用于增强含纤维素材料的降解或转化的组合物和方法 |
US7923236B2 (en) * | 2007-08-02 | 2011-04-12 | Dyadic International (Usa), Inc. | Fungal enzymes |
CA2736661A1 (en) | 2007-09-07 | 2009-03-12 | Dyadic International, Inc. | Novel fungal enzymes |
BRPI1009488B1 (pt) | 2009-03-16 | 2020-09-15 | Danisco Us Inc | Cepa de fungo hospedeiro e métodos para produção homóloga e/ou heteróloga de uma proteína pura com uma pureza superior a 75%, para produção de misturas artificiais de proteínas e para triagem simplificada de cepas que expressam 5 funcionalmente uma enzima desejada |
CA2757040C (en) | 2009-03-31 | 2016-02-09 | Codexis, Inc. | Improved endoglucanases |
US9012186B2 (en) | 2009-04-27 | 2015-04-21 | The Board Of Trustees Of The University Of Illinois | Hemicellulose-degrading enzymes |
FI121712B (fi) * | 2009-04-30 | 2011-03-15 | Ab Enzymes Oy | Uusi sieniperäinen proteaasi ja sen käyttö |
FI121711B (fi) * | 2009-04-30 | 2011-03-15 | Ab Enzymes Oy | Sieniperäinen seriiniproteaasi ja sen käyttö |
BRPI1010696A2 (pt) | 2009-06-16 | 2016-10-11 | Codexis Inc | método de produção de um polipeptídeo de b-glicosidade com termoatividade aperfeiçoada, polipeptídeo, proteína e seus usos |
FI121851B (fi) * | 2009-07-08 | 2011-05-13 | Ab Enzymes Oy | Sieniperäinen proteaasi ja sen käyttö |
CA2774776A1 (en) | 2009-09-23 | 2011-03-31 | Danisco Us Inc. | Novel glycosyl hydrolase enzymes and uses thereof |
CA2776170C (en) | 2009-09-30 | 2016-01-05 | Codexis, Inc. | Recombinant c1 .beta.-glucosidase for production of sugars from cellulosic biomass |
RU2012126131A (ru) | 2009-11-25 | 2013-12-27 | Кодексис, Инк. | РЕКОМБИНАНТНЫЕ ВАРИАНТЫ β-ГЛЮКОЗИДАЗЫ ДЛЯ ПРОДУКЦИИ РАСТВОРИМЫХ САХАРОВ ИЗ ЦЕЛЛЮЛОЗНОЙ БИОМАССЫ |
BR112012012192A2 (pt) | 2009-11-25 | 2017-07-04 | Codexis Inc | b-glicosidades variantes recombinantes thermoascus aurantiacus para produção de açúcares fermentáveis a partir da biomassa celulósica |
DE102009055015B4 (de) * | 2009-12-18 | 2013-12-24 | Technische Universität Dresden | Hefe-Sekretionssystem auf Basis einer Signalsequenz aus der Hydrophobin 1-Gensequenz von Trichoderma reesei |
US10138499B2 (en) | 2009-12-23 | 2018-11-27 | Danisco Us Inc. | Methods for improving the efficiency of simultaneous saccharification and fermentation reactions |
FI122937B (fi) | 2009-12-30 | 2012-09-14 | Roal Oy | Menetelmä selluloosamateriaalin käsittelemiseksi sekä tässä käyttökelpoiset CBH II/Cel6A entsyymit |
US9493802B2 (en) | 2010-08-20 | 2016-11-15 | Codexis, Inc. | Use of glycohydrolase 61 protein variants with improved thermostability for processing cellulose |
WO2012024662A2 (en) * | 2010-08-20 | 2012-02-23 | Codexis, Inc. | Expression constructs comprising fungal promoters |
CN103562402A (zh) * | 2010-10-20 | 2014-02-05 | 丹尼斯科美国公司 | 热稳定性木霉属纤维素酶 |
FI123942B (fi) | 2010-10-29 | 2013-12-31 | Ab Enzymes Oy | Sieniperäisen seriiniproteaasin variantteja |
US20130344541A1 (en) | 2010-11-02 | 2013-12-26 | Codexis, Inc. | Compositions and methods for production of fermentable sugars |
HUE031303T2 (hu) | 2010-11-02 | 2017-07-28 | Codexis Inc | Javított gombatörzsek |
WO2012068236A2 (en) * | 2010-11-16 | 2012-05-24 | Dyadic International (Usa) Inc. | Novel fungal oxidoreductases |
CN102154237A (zh) * | 2011-01-04 | 2011-08-17 | 深圳市绿微康生物工程有限公司 | 一种有效降低动物体内胆固醇、血脂水平的脂肪酶及其应用 |
CN102154340B (zh) * | 2011-01-04 | 2013-07-10 | 深圳市绿微康生物工程有限公司 | 一种获得高效表达脂肪酶菌株的方法 |
CN102154238A (zh) * | 2011-01-04 | 2011-08-17 | 深圳市绿微康生物工程有限公司 | 一种有效降低人体胆固醇、血脂水平的脂肪酶及其应用 |
CN102154358B (zh) * | 2011-01-04 | 2013-07-10 | 深圳市绿微康生物工程有限公司 | 一种高效表达的产脂肪酶基因质粒的构建方法 |
DK2686434T3 (da) | 2011-03-17 | 2021-10-11 | Danisco Us Inc | Fremgangsmåde til reduktion af viskositet i saccharificeringsproces |
CN103492561A (zh) | 2011-03-17 | 2014-01-01 | 丹尼斯科美国公司 | 纤维素酶组合物和使用相同组合物用于改善木质纤维素生物质转化成可发酵糖的方法 |
FI123425B (fi) | 2011-03-31 | 2013-04-30 | Ab Enzymes Oy | Proteaasientsyymi ja sen käytöt |
CN103014036A (zh) * | 2011-07-01 | 2013-04-03 | 深圳市绿微康生物工程有限公司 | 改型扩展青霉脂肪酶基因及其构建和表达方法 |
WO2013028701A1 (en) | 2011-08-22 | 2013-02-28 | Codexis, Inc. | Gh61 glycoside hydrolase protein variants and cofactors that enhance gh61 activity |
US20130084608A1 (en) | 2011-09-30 | 2013-04-04 | Codexis, Inc. | Fungal proteases |
WO2013067026A1 (en) | 2011-10-31 | 2013-05-10 | Bp Corporation North America Inc. | Use of plant promoters in filamentous fungi |
CA2851308A1 (en) | 2011-10-31 | 2013-05-10 | Bp Corporation North America Inc. | Use of mammalian promoters in filamentous fungi |
US20140234897A1 (en) * | 2011-11-04 | 2014-08-21 | Novozymes Inc. | Polypeptides Having Cellobiohydrolase Activity and Polynucleotides Encoding Same |
WO2013096244A1 (en) | 2011-12-20 | 2013-06-27 | Codexis, Inc. | Endoglucanase 1b (eg1b) variants |
BR112014031024A2 (pt) | 2012-06-11 | 2017-06-27 | Codexis Inc | xilanases e xilosidases fúngicas. |
WO2014070856A2 (en) | 2012-11-02 | 2014-05-08 | Bp Corporation North America Inc. | Thermotolerant beta-glucosidase variants |
WO2014145768A2 (en) | 2013-03-15 | 2014-09-18 | Bp Corporation North America Inc. | Use of non-fungal 5' utrs in filamentous fungi |
ES2521940B1 (es) | 2013-05-10 | 2015-09-09 | Abengoa Bioenergía Nuevas Tecnologías, S.A. | Variantes de beta-glucosidasa con actividad de transglicosilación reducida |
DK3000880T3 (en) | 2013-05-20 | 2019-04-15 | Abengoa Bioenergia Nuevas Tecnologias Sa | EXPRESSION OF RECOMBINANT BETA-XYLOSIDASE ENZYMERS |
ES2527368B1 (es) | 2013-07-22 | 2015-10-27 | Abengoa Bioenergía Nuevas Tecnologías, S.A. | Célula hospedadora de Myceliophthora thermophila que expresa una enzima alfa-xilosidasa heteróloga y su uso en un procedimiento de degradación de biomasa |
US20170166939A1 (en) | 2013-11-26 | 2017-06-15 | Novozymes A/S | Enzyme Compositions and Uses Thereof |
ES2542621B1 (es) | 2014-02-07 | 2016-08-16 | Abengoa Bioenergía Nuevas Tecnologías, S.A. | Polipéptidos con actividad polisacárido monooxigenasa y su uso para la producción de azúcares fermentables |
WO2015187935A1 (en) | 2014-06-06 | 2015-12-10 | Novozymes A/S | Enzyme compositions and uses thereof |
BR112017019280A2 (pt) | 2015-03-11 | 2018-05-02 | Genencor Int Bv | atividade enzimática de mono-oxigenase de polissacarídeo lítica |
CN107531762A (zh) | 2015-05-07 | 2018-01-02 | 科德克希思公司 | 青霉素g酰化酶 |
DK3313999T3 (da) | 2015-06-24 | 2021-08-02 | Genencor Int Bv | Polypeptider med demethyleringsaktivitet |
BR112018001855A2 (pt) | 2015-07-29 | 2018-09-25 | Abengoa Bioenergia Nuevas Tecnologias Sa | expressão de enzimas beta-xilosidase recombinantes |
MX2018006536A (es) | 2015-12-02 | 2018-08-15 | Basf Se | Metodo para producir proteinas en hongos filamentosos con accion clr1 reducida. |
MX2018006535A (es) | 2015-12-02 | 2018-08-15 | Basf Se | Metodo para producir proteinas en hongos filamentosos con accion clr2 reducida. |
JP6821598B2 (ja) | 2015-12-07 | 2021-01-27 | ザイマージェン インコーポレイテッド | Corynebacterium glutamicum由来のプロモーター |
US9988624B2 (en) | 2015-12-07 | 2018-06-05 | Zymergen Inc. | Microbial strain improvement by a HTP genomic engineering platform |
US11208649B2 (en) | 2015-12-07 | 2021-12-28 | Zymergen Inc. | HTP genomic engineering platform |
DK3419992T3 (da) | 2016-02-22 | 2021-02-15 | Danisco Us Inc | Svampesystem til proteinproduktion med højt udbytte |
CA3021009A1 (en) | 2016-05-05 | 2017-11-09 | Codexis, Inc. | Penicillin-g acylases |
ES2647322B1 (es) | 2016-05-20 | 2018-10-05 | Abengoa Bioenergía Nuevas Tecnologías, S.A. | Célula hospedadora Myceliophthora thermophila con actividad celulolítica mejorada y composiciones enzimáticas obtenidas por la misma |
KR102345899B1 (ko) | 2016-06-30 | 2021-12-31 | 지머젠 인코포레이티드 | 박테리아 헤모글로빈 라이브러리를 생성하는 방법 및 이의 용도 |
US10544411B2 (en) | 2016-06-30 | 2020-01-28 | Zymergen Inc. | Methods for generating a glucose permease library and uses thereof |
RU2019102378A (ru) | 2016-06-30 | 2020-07-30 | ДАНИСКО ЮЭс ИНК. | Аспарагиновые протеазы |
MX2019008096A (es) | 2017-01-05 | 2019-09-04 | Codexis Inc | Penicilina g acilasas. |
DK3378936T3 (da) * | 2017-03-24 | 2021-08-16 | Clariant Int Ltd | Cellulase, som er egnet til anvendelse i detergentsammensætninger |
CN110719956A (zh) | 2017-06-06 | 2020-01-21 | 齐默尔根公司 | 用于改良真菌菌株的高通量基因组工程改造平台 |
JP7122009B2 (ja) | 2017-06-14 | 2022-08-19 | コデクシス, インコーポレイテッド | 産業上の生体触媒作用のための操作されたトランスアミナーゼポリペプチド |
CN111051506B (zh) | 2017-06-27 | 2023-11-24 | 科德克希思公司 | 青霉素g酰化酶 |
CN111315408A (zh) * | 2017-08-21 | 2020-06-19 | 二进国际有限公司 | 在嗜热毁丝霉(myceliophthora thermophila)中生产流感疫苗 |
US11319531B2 (en) | 2017-11-07 | 2022-05-03 | Codexis, Inc. | Transglutaminase variants |
CN109988715B (zh) * | 2017-12-29 | 2022-03-01 | 青岛蔚蓝生物集团有限公司 | 一种高产木聚糖酶的突变菌株及其应用 |
CN110093332B (zh) * | 2018-01-30 | 2021-12-28 | 青岛蔚蓝生物集团有限公司 | 一种纤维素酶突变体及其高产菌株 |
US11028401B2 (en) | 2018-06-06 | 2021-06-08 | Zymergen Inc. | Manipulation of genes involved in signal transduction to control fungal morphology during fermentation and production |
KR20210032417A (ko) | 2018-07-09 | 2021-03-24 | 코덱시스, 인코포레이티드 | 조작된 데옥시리보스-포스페이트 알돌라아제 |
CN108913713A (zh) * | 2018-07-24 | 2018-11-30 | 中国农业科学院饲料研究所 | 提高里氏木霉转化效率的重组表达载体及应用 |
JP2023521772A (ja) | 2020-04-10 | 2023-05-25 | コデクシス, インコーポレイテッド | 操作されたトランスアミナーゼポリペプチド |
US11479779B2 (en) | 2020-07-31 | 2022-10-25 | Zymergen Inc. | Systems and methods for high-throughput automated strain generation for non-sporulating fungi |
CN113403209B (zh) * | 2021-07-30 | 2022-08-26 | 西南大学 | 天冬氨酸蛋白酶基因在改良球孢白僵菌菌株中的应用 |
Family Cites Families (111)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2974001A (en) * | 1957-07-20 | 1961-03-07 | Kalle Ag | Process and compositions for enzymatic desizing and bleaching of textiles |
GB1368599A (en) | 1970-09-29 | 1974-10-02 | Unilever Ltd | Softening compositions |
JPS5028515B2 (ko) * | 1971-09-30 | 1975-09-16 | ||
US3966543A (en) * | 1972-10-30 | 1976-06-29 | Baxter Laboratories, Inc. | Enzyme-treated paper |
US4081328A (en) * | 1975-10-23 | 1978-03-28 | Stanford Research Institute | Production of cellulase by a thermophilic thielavia terrestris |
DK187280A (da) * | 1980-04-30 | 1981-10-31 | Novo Industri As | Ruhedsreducerende middel til et fuldvaskemiddel fuldvaskemiddel og fuldvaskemetode |
GB2094826B (en) | 1981-03-05 | 1985-06-12 | Kao Corp | Cellulase enzyme detergent composition |
JPS591598A (ja) * | 1982-06-25 | 1984-01-06 | 花王株式会社 | 洗浄剤組成物 |
US4486533A (en) * | 1982-09-02 | 1984-12-04 | St. Louis University | Filamentous fungi functional replicating extrachromosomal element |
NL8300698A (nl) * | 1983-02-24 | 1984-09-17 | Univ Leiden | Werkwijze voor het inbouwen van vreemd dna in het genoom van tweezaadlobbige planten; agrobacterium tumefaciens bacterien en werkwijze voor het produceren daarvan; planten en plantecellen met gewijzigde genetische eigenschappen; werkwijze voor het bereiden van chemische en/of farmaceutische produkten. |
GB8306645D0 (en) * | 1983-03-10 | 1983-04-13 | Unilever Plc | Detergent compositions |
US4462307A (en) * | 1983-05-23 | 1984-07-31 | Pet Incorporated | Humpback oven-broiler |
GB8421800D0 (en) * | 1984-08-29 | 1984-10-03 | Unilever Plc | Detergent compositions |
GB8422238D0 (en) | 1984-09-03 | 1984-10-10 | Neuberger M S | Chimeric proteins |
US4816405A (en) * | 1984-10-24 | 1989-03-28 | The Regents Of The University Of California | Vectors for transformation by ascomycetes |
US4885249A (en) * | 1984-12-05 | 1989-12-05 | Allelix, Inc. | Aspergillus niger transformation system |
US4610800A (en) * | 1985-01-25 | 1986-09-09 | Genex Corporation | Method for unclogging drainage pipes |
US5198345A (en) * | 1985-04-15 | 1993-03-30 | Gist-Brocades N.V. | Vectors in use in filamentous fungi |
US5364770A (en) * | 1985-08-29 | 1994-11-15 | Genencor International Inc. | Heterologous polypeptides expressed in aspergillus |
DE3650202T3 (de) | 1985-08-29 | 2004-06-03 | Genencor International, Inc., South San Francisco | Expression von heterologen Polypeptiden in filamentösen Pilzen, Verfahren zu deren Herstellung und Vektoren zu deren Herstellung. |
DK163591C (da) | 1985-10-08 | 1992-08-24 | Novo Nordisk As | Fremgangsmaade til behandling af et tekstilstof med en cellulase |
US4935349A (en) * | 1986-01-17 | 1990-06-19 | Zymogenetics, Inc. | Expression of higher eucaryotic genes in aspergillus |
US5536661A (en) * | 1987-03-10 | 1996-07-16 | Novo Nordisk A/S | Process for the production of protein products in aspergillus |
US5766912A (en) * | 1986-03-17 | 1998-06-16 | Novo Nordisk A/S | Humicola lipase produced in aspergillus |
GB8607679D0 (en) | 1986-03-27 | 1986-04-30 | Winter G P | Recombinant dna product |
US5763192A (en) * | 1986-11-20 | 1998-06-09 | Ixsys, Incorporated | Process for obtaining DNA, RNA, peptides, polypeptides, or protein, by recombinant DNA technique |
US5252726A (en) * | 1987-09-04 | 1993-10-12 | Novo Nordisk A/S | Promoters for use in aspergillus |
US4832864A (en) * | 1987-09-15 | 1989-05-23 | Ecolab Inc. | Compositions and methods that introduce variations in color density into cellulosic fabrics, particularly indigo dyed denim |
US5223409A (en) * | 1988-09-02 | 1993-06-29 | Protein Engineering Corp. | Directed evolution of novel binding proteins |
US5006126A (en) * | 1988-09-15 | 1991-04-09 | Ecolab Inc. | Cellulase compositions and methods that introduce variations in color density into cellulosic fabrics, particularly indigo dyed denim |
US5122159A (en) * | 1988-09-15 | 1992-06-16 | Ecolab Inc. | Cellulase compositions and methods that introduce variations in color density into cellulosic fabrics, particularly indigo dyed denim |
IL162181A (en) | 1988-12-28 | 2006-04-10 | Pdl Biopharma Inc | A method of producing humanized immunoglubulin, and polynucleotides encoding the same |
US5571697A (en) * | 1989-05-05 | 1996-11-05 | Baylor College Of Medicine Texas Medical Center | Expression of processed recombinant lactoferrin and lactoferrin polypeptide fragments from a fusion product in Aspergillus |
WO1991000092A1 (en) | 1989-06-13 | 1991-01-10 | Smithkline Beecham Corporation | Inhibition of interleukin-1 and tumor necrosis factor production by monocytes and/or macrophages |
IE81016B1 (en) | 1989-07-07 | 1999-09-22 | Unilever Plc | Process for preparing a protein by a fungus transformed by a multicopy integration of an expression vector |
US5120463A (en) * | 1989-10-19 | 1992-06-09 | Genencor International, Inc. | Degradation resistant detergent compositions based on cellulase enzymes |
GB8928874D0 (en) | 1989-12-21 | 1990-02-28 | Celltech Ltd | Humanised antibodies |
AU7493491A (en) * | 1990-03-13 | 1991-10-10 | Hawaii Biotechnology Group, Inc. | (neurospora) expression system |
DK115890D0 (da) | 1990-05-09 | 1990-05-09 | Novo Nordisk As | Enzym |
GB9015198D0 (en) * | 1990-07-10 | 1990-08-29 | Brien Caroline J O | Binding substance |
ATE130049T1 (de) * | 1990-07-13 | 1995-11-15 | Transkaryotic Therapies Inc | Verfahren zum absuchen einer bibliothek. |
EP0542810A1 (en) * | 1990-08-02 | 1993-05-26 | B.R. Centre Limited | Methods for the production of proteins with a desired function |
JP3133774B2 (ja) * | 1990-10-04 | 2001-02-13 | 日本食品化工株式会社 | 新規な宿主−ベクター系 |
US5290474A (en) * | 1990-10-05 | 1994-03-01 | Genencor International, Inc. | Detergent composition for treating cotton-containing fabrics containing a surfactant and a cellulase composition containing endolucanase III from trichoderma ssp |
US5780279A (en) * | 1990-12-03 | 1998-07-14 | Genentech, Inc. | Method of selection of proteolytic cleavage sites by directed evolution and phagemid display |
DE69133100T3 (de) * | 1990-12-10 | 2015-09-03 | Danisco Us Inc. | Verbesserte saccharifizierung von zellulose durch klonierung und vervielfältigung des beta-glukosidase genes aus trichoderma reesei |
GB9102635D0 (en) | 1991-02-07 | 1991-03-27 | British Bio Technology | Compounds |
US5858657A (en) * | 1992-05-15 | 1999-01-12 | Medical Research Council | Methods for producing members of specific binding pairs |
DE69230142T2 (de) * | 1991-05-15 | 2000-03-09 | Cambridge Antibody Tech | Verfahren zur herstellung von spezifischen bindungspaargliedern |
US5565332A (en) * | 1991-09-23 | 1996-10-15 | Medical Research Council | Production of chimeric antibodies - a combinatorial approach |
US5516670A (en) * | 1991-09-30 | 1996-05-14 | Kuehnle; Adelheid R. | Magnetophoretic particle delivery method and apparatus for the treatment of cells |
BE1005432A4 (nl) | 1991-10-01 | 1993-07-20 | Dsm Nv | Expressiecassette voor heterologe eiwitten. |
WO1993012237A1 (en) * | 1991-12-09 | 1993-06-24 | Unilever N.V. | Process for producing/secreting a protein by a transformed mould using expression/secretion regulating regions derived from an aspergillus endoxylanase ii gene |
CA2062023A1 (en) * | 1992-02-10 | 1993-08-11 | Jagroop S. Dahiya | Novel fungal strains and use thereof in antibiotic production |
CA2142602A1 (en) | 1992-08-19 | 1994-03-03 | Tiina Hannele Nakari | Fungal promoters active in the presence of glucose |
PT663953E (pt) * | 1992-09-04 | 2002-05-31 | Scripps Research Inst | Fagemideos que co-expressam um receptor de superficie e uma proteina heterologa de superficie |
JPH08504327A (ja) | 1992-12-10 | 1996-05-14 | ギスト ブロカデス ナムローゼ フェンノートシャップ | 糸状菌における異種タンパクの生産 |
GB9316883D0 (en) * | 1993-08-13 | 1993-09-29 | Univ Leeds | Production of heterologous peptices |
EP0726940A4 (en) * | 1993-11-02 | 2001-05-23 | Merck & Co Inc | DNA ENCODING A TRIOL-POLYCETIDE-SYNTHASE |
US5605793A (en) * | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
US5820866A (en) * | 1994-03-04 | 1998-10-13 | National Jewish Center For Immunology And Respiratory Medicine | Product and process for T cell regulation |
GB2289218A (en) | 1994-05-06 | 1995-11-15 | Merck & Co Inc | Inhibition of TNFalpha production with agonists of the A2b subtype of the adenosine receptor |
WO1995035385A1 (en) * | 1994-06-17 | 1995-12-28 | Novo Nordisk A/S | A FUNGUS WHEREIN THE areA GENE HAS BEEN MODIFIED AND AN areA GENE FROM ASPERGILLUS ORYZAE |
US6060305A (en) * | 1994-06-30 | 2000-05-09 | Novo Nordisk Biotech, Inc. | Non-toxic, non-toxigenic, non-pathogenic Fusarium expression system |
ATE294871T1 (de) * | 1994-06-30 | 2005-05-15 | Novozymes Biotech Inc | Nicht-toxisches, nicht-toxigenes, nicht- pathogenes fusarium expressionssystem und darin zu verwendende promotoren und terminatoren |
EP0770090A1 (en) | 1994-07-13 | 1997-05-02 | Cornell Research Foundation, Inc. | Epstein-barr virus nuclear antigen 1 protein and its expression and recovery |
US5602004A (en) * | 1994-07-20 | 1997-02-11 | Novo Nordisk Biotech, Inc. | Thermophilic fungal expression system |
JP3974171B2 (ja) | 1995-03-20 | 2007-09-12 | ノボザイムス アクティーゼルスカブ | 低下したレベルのメタロプロテアーゼを発見する宿主細胞及びその細胞をタンパク質の生産に用いる方法 |
US6046021A (en) * | 1995-04-12 | 2000-04-04 | Biolog, Inc. | Comparative phenotype analysis of two or more microorganisms using a plurality of substrates within a multiwell testing device |
US5824485A (en) * | 1995-04-24 | 1998-10-20 | Chromaxome Corporation | Methods for generating and screening novel metabolic pathways |
US6057103A (en) * | 1995-07-18 | 2000-05-02 | Diversa Corporation | Screening for novel bioactivities |
US6030779A (en) * | 1995-07-18 | 2000-02-29 | Diversa Corporation | Screening for novel bioactivities |
US5958672A (en) * | 1995-07-18 | 1999-09-28 | Diversa Corporation | Protein activity screening of clones having DNA from uncultivated microorganisms |
FI103133B1 (fi) | 1995-09-01 | 1999-04-30 | Valtion Teknillinen | Menetelmä glukoosirepression modifioimiseksi |
AU7294396A (en) | 1995-10-13 | 1997-04-30 | Gist-Brocades B.V. | Protein detection |
US5830696A (en) * | 1996-12-05 | 1998-11-03 | Diversa Corporation | Directed evolution of thermophilic enzymes |
US5939250A (en) * | 1995-12-07 | 1999-08-17 | Diversa Corporation | Production of enzymes having desired activities by mutagenesis |
US5776730A (en) * | 1995-12-15 | 1998-07-07 | University Of Hawaii | Neurospora hosts for the production of recombinant proteins, and methods for producing same |
EP1605050A3 (en) * | 1996-01-19 | 2006-03-22 | Novozymes Biotech, Inc. | Morphological mutants of filamentous fungi |
JP2000504939A (ja) | 1996-01-26 | 2000-04-25 | ノボ ノルディスク アクティーゼルスカブ | 衛生紙の製造 |
US5753477A (en) * | 1996-03-19 | 1998-05-19 | University Technology Corporation | Magneto-biolistic methods |
US5783431A (en) * | 1996-04-24 | 1998-07-21 | Chromaxome Corporation | Methods for generating and screening novel metabolic pathways |
US7883872B2 (en) * | 1996-10-10 | 2011-02-08 | Dyadic International (Usa), Inc. | Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose |
US5811381A (en) * | 1996-10-10 | 1998-09-22 | Mark A. Emalfarb | Cellulase compositions and methods of use |
US5879921A (en) * | 1996-11-07 | 1999-03-09 | Novo Nordisk A/S | Recombinant expression of a glucose oxidase from a cladosporium strain |
AU5794598A (en) * | 1996-12-13 | 1998-07-03 | Patricia Tekamp-Olson | Method for expression of heterologous proteins in yeast |
US5989814A (en) * | 1997-04-01 | 1999-11-23 | Reagents Of The University Of California | Screening methods in eucaryotic cells |
US6573068B1 (en) * | 1997-11-13 | 2003-06-03 | Genset, S. A. | Claudin-50 protein |
ES2143408B1 (es) | 1998-04-02 | 2000-12-01 | Urquima Sa | Promotor y construcciones para expresion de proteinas recombinantes en hongos filamentosos. |
US5914245A (en) | 1998-04-20 | 1999-06-22 | Kairos Scientific Inc. | Solid phase enzyme kinetics screening in microcolonies |
US6413776B1 (en) | 1998-06-12 | 2002-07-02 | Galapagos Geonomics N.V. | High throughput screening of gene function using adenoviral libraries for functional genomics applications |
EP1088229A4 (en) | 1998-06-25 | 2002-05-08 | Caliper Techn Corp | HIGH THROUGHPUT METHODS, SYSTEMS AND APPARATUS FOR CELL SCREENING TEST |
AU761570B2 (en) | 1998-06-29 | 2003-06-05 | Bristol-Myers Squibb Company | Methods for generating highly diverse libraries |
EP1117808B1 (en) * | 1998-10-06 | 2004-12-29 | Mark Aaron Emalfarb | Transformation system in the field of filamentous fungal hosts: in chrysosporium |
US6518042B1 (en) * | 1999-02-24 | 2003-02-11 | Novozymes A/S | Process for making DNA libraries in filamentous fungal cells using a novel cloned gene involved in the mismatch repair system of filamentous fungal cells |
JP4620253B2 (ja) | 1999-03-22 | 2011-01-26 | ノボザイムス,インコーポレイティド | 菌類細胞中で遺伝子を発現させるためのプロモーター |
US6121034A (en) * | 1999-05-13 | 2000-09-19 | Her Majesty The Queen In Right Of Canada, As Represented By The Minister Of Agriculture And Agri-Food | Coniothyrium minitans xylanase gene Cxy1 |
AU4352700A (en) | 1999-10-06 | 2001-05-10 | Mark Aaron Emalfarb | High-throughput screening of expressed dna libraries in filamentous fungi |
DK1276876T3 (da) * | 2000-04-13 | 2007-07-16 | Mark Aaron Emalfarb | Ekspressionsregulerende sekvenser fra den filamentöse svamp Chrysosporium |
MXPA01012905A (es) | 2000-04-13 | 2002-11-22 | Mark Aaron Emalfarb | Clasificacion de alto rendimiento de bibliotecas de adn expresado en hongo filamentos. |
KR20020026456A (ko) * | 2000-04-13 | 2002-04-10 | 에말파브 마크 아론 | 사상균에서 발현된 dna 라이브러리의 고산출량 스크리닝 |
WO2002064741A2 (en) * | 2001-02-13 | 2002-08-22 | Diadexus, Inc. | Compositions and methods relating to breast specific genes and proteins |
AU2003279841A1 (en) | 2002-10-07 | 2004-05-04 | Zymogenetics, Inc. | Uses of human zven antagonists |
DK1578964T4 (da) * | 2002-12-20 | 2013-12-02 | Novozymes As | Polypeptider med cellobiohydrolase II-aktivitet og polynukleotider, der koder for samme |
CN108728428A (zh) * | 2004-01-30 | 2018-11-02 | 诺维信股份有限公司 | 具有分解纤维增强活性的多肽及其编码多核苷酸 |
US7271244B2 (en) * | 2004-02-06 | 2007-09-18 | Novozymes, Inc. | Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same |
US7332319B2 (en) * | 2004-05-27 | 2008-02-19 | Genencor International, Inc. | Heterologous alpha amylase expression in Aspergillus |
US20060105361A1 (en) * | 2004-08-24 | 2006-05-18 | Rothstein Rodney J | Donor yeast strain for transfer of genetic material |
DK1836299T4 (da) * | 2005-01-06 | 2014-03-31 | Novozymes Inc | Polypeptider, der har cellobiohydrolaseaktivitet, og polynukleotider, der koder for disse |
RU2441912C2 (ru) * | 2005-09-30 | 2012-02-10 | Новозаймз, Инк. | Способы усиления деградации или превращения целлюлозного материала |
US7923236B2 (en) * | 2007-08-02 | 2011-04-12 | Dyadic International (Usa), Inc. | Fungal enzymes |
-
1999
- 1999-10-06 EP EP99949459A patent/EP1117808B1/en not_active Expired - Lifetime
- 1999-10-06 KR KR1020017004377A patent/KR100618495B1/ko not_active IP Right Cessation
- 1999-10-06 CN CNB99814133XA patent/CN1230546C/zh not_active Expired - Lifetime
- 1999-10-06 DE DE69922978T patent/DE69922978T2/de not_active Expired - Lifetime
- 1999-10-06 EA EA200100419A patent/EA005682B1/ru not_active IP Right Cessation
- 1999-10-06 BR BRPI9914278A patent/BRPI9914278B1/pt not_active IP Right Cessation
- 1999-10-06 AU AU62326/99A patent/AU771539C/en not_active Ceased
- 1999-10-06 ES ES99949459T patent/ES2237159T3/es not_active Expired - Lifetime
- 1999-10-06 DK DK99949459T patent/DK1117808T3/da active
- 1999-10-06 WO PCT/NL1999/000618 patent/WO2000020555A2/en active IP Right Grant
- 1999-10-06 CA CA2345356A patent/CA2345356C/en not_active Expired - Lifetime
- 1999-10-06 AT AT99949459T patent/ATE286136T1/de not_active IP Right Cessation
-
2000
- 2000-04-13 US US09/548,938 patent/US6573086B1/en not_active Expired - Lifetime
-
2003
- 2003-03-21 US US10/394,568 patent/US7399627B2/en not_active Expired - Fee Related
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2008
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US7399627B2 (en) | 2008-07-15 |
AU771539B2 (en) | 2004-03-25 |
WO2000020555A2 (en) | 2000-04-13 |
BRPI9914278B1 (pt) | 2016-03-08 |
ES2237159T3 (es) | 2005-07-16 |
CN1230546C (zh) | 2005-12-07 |
ATE286136T1 (de) | 2005-01-15 |
WO2000020555A3 (en) | 2000-10-05 |
BR9914278A (pt) | 2001-06-19 |
US20040002136A1 (en) | 2004-01-01 |
DE69922978D1 (de) | 2005-02-03 |
US20080194005A1 (en) | 2008-08-14 |
DE69922978T2 (de) | 2005-12-08 |
EA200100419A1 (ru) | 2001-10-22 |
US20140127788A1 (en) | 2014-05-08 |
CA2345356A1 (en) | 2000-04-13 |
US8268585B2 (en) | 2012-09-18 |
CN1330717A (zh) | 2002-01-09 |
KR20010103596A (ko) | 2001-11-23 |
AU6232699A (en) | 2000-04-26 |
US8871493B2 (en) | 2014-10-28 |
EP1117808B1 (en) | 2004-12-29 |
US6573086B1 (en) | 2003-06-03 |
EA005682B1 (ru) | 2005-04-28 |
CA2345356C (en) | 2012-10-02 |
AU771539C (en) | 2005-01-13 |
EP1117808A2 (en) | 2001-07-25 |
DK1117808T3 (da) | 2005-04-25 |
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