EA200100419A1 - Трансформационная система в области грибов гифомицетов-хозяев - Google Patents

Трансформационная система в области грибов гифомицетов-хозяев

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Publication number
EA200100419A1
EA200100419A1 EA200100419A EA200100419A EA200100419A1 EA 200100419 A1 EA200100419 A1 EA 200100419A1 EA 200100419 A EA200100419 A EA 200100419A EA 200100419 A EA200100419 A EA 200100419A EA 200100419 A1 EA200100419 A1 EA 200100419A1
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Eurasian Patent Office
Prior art keywords
chrysosporium
hosts
sequences
field
transformation system
Prior art date
Application number
EA200100419A
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English (en)
Other versions
EA005682B1 (ru
Inventor
Марк Аарон Эмалфарб
Ричард Пол Берлингейм
Филип Терри Олсон
Аркадий Пантелеймонович Синицин
Мартин Парриш
Жан Кристоф Буссон
Кристин Мари Пиннонен
Питер Ян Пант
Корнелия Мария Йоханна Ван Зейл
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Марк Аарон Эмалфарб
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by Марк Аарон Эмалфарб filed Critical Марк Аарон Эмалфарб
Publication of EA200100419A1 publication Critical patent/EA200100419A1/ru
Publication of EA005682B1 publication Critical patent/EA005682B1/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2462—Lysozyme (3.2.1.17)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C12N9/2482—Endo-1,4-beta-xylanase (3.2.1.8)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
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    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
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  • Biomedical Technology (AREA)
  • Microbiology (AREA)
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  • Medicinal Chemistry (AREA)
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  • Plant Pathology (AREA)
  • Biophysics (AREA)
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  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Radio Relay Systems (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

Описана новая трансформационная система в области использования грибков-гифомицетов в качестве хозяев для экспрессии и секреции гетерологичных белков или полипептидов. Также настоящее изобретение охватывает способ получения больших количеств полипептида или белка в промышленном масштабе. Данная система включает трансформированный или трансфицированный грибковый штамм рода Chrysosporium, более конкретно Chrysosporium lucknowense, и производные от него мутанты. Также оно охватывает трансформанты, включающие кодирующие последовательности Chrysosporium, а также регулирующие экспрессию последовательности генов Chrysosporium.Отчет о международном поиске был опубликован 2000.10.05.
EA200100419A 1998-10-06 1999-10-06 Трансформированные грибы, в частности рода chrysosporium, способные к синтезу гетерологичных полипептидов EA005682B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EPPCT/EP98/06496 1998-10-06
PCT/NL1999/000618 WO2000020555A2 (en) 1998-10-06 1999-10-06 Transformation system in the field of filamentous fungal hosts: in chrysosporium

Publications (2)

Publication Number Publication Date
EA200100419A1 true EA200100419A1 (ru) 2001-10-22
EA005682B1 EA005682B1 (ru) 2005-04-28

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
EA200100419A EA005682B1 (ru) 1998-10-06 1999-10-06 Трансформированные грибы, в частности рода chrysosporium, способные к синтезу гетерологичных полипептидов

Country Status (13)

Country Link
US (4) US6573086B1 (ru)
EP (1) EP1117808B1 (ru)
KR (1) KR100618495B1 (ru)
CN (1) CN1230546C (ru)
AT (1) ATE286136T1 (ru)
AU (1) AU771539C (ru)
BR (1) BRPI9914278B1 (ru)
CA (1) CA2345356C (ru)
DE (1) DE69922978T2 (ru)
DK (1) DK1117808T3 (ru)
EA (1) EA005682B1 (ru)
ES (1) ES2237159T3 (ru)
WO (1) WO2000020555A2 (ru)

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US8268585B2 (en) 2012-09-18
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CA2345356A1 (en) 2000-04-13
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ATE286136T1 (de) 2005-01-15
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