ES2143408B1 - Promotor y construcciones para expresion de proteinas recombinantes en hongos filamentosos. - Google Patents

Promotor y construcciones para expresion de proteinas recombinantes en hongos filamentosos.

Info

Publication number
ES2143408B1
ES2143408B1 ES9800699A ES9800699A ES2143408B1 ES 2143408 B1 ES2143408 B1 ES 2143408B1 ES 9800699 A ES9800699 A ES 9800699A ES 9800699 A ES9800699 A ES 9800699A ES 2143408 B1 ES2143408 B1 ES 2143408B1
Authority
ES
Spain
Prior art keywords
promoter
expression
recombinant proteins
gene
fungi
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES9800699A
Other languages
English (en)
Other versions
ES2143408A1 (es
Inventor
Silva Rosa Elena Cardoza
Lorenzo Fco Jose Moralejo
Martin Santiago Gutierrez
Javier Casqueiro
Maria Jose Hijarrubia
Juan Francisco Martin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Urquima SA
Original Assignee
Urquima SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Urquima SA filed Critical Urquima SA
Priority to ES9800699A priority Critical patent/ES2143408B1/es
Priority to CA002325571A priority patent/CA2325571A1/en
Priority to EP99944133A priority patent/EP1084262A1/en
Priority to PCT/EP1999/002243 priority patent/WO1999051756A2/en
Priority to JP2000542467A priority patent/JP2002510497A/ja
Priority to AU42575/99A priority patent/AU4257599A/en
Publication of ES2143408A1 publication Critical patent/ES2143408A1/es
Application granted granted Critical
Publication of ES2143408B1 publication Critical patent/ES2143408B1/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0016—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with NAD or NADP as acceptor (1.4.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C07K14/43—Sweetening agents, e.g. thaumatin, monellin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Abstract

Promotor y construcciones para expresión de proteínas recombinantes en hongos filamentosos. Se describe un nuevo promotor para la expresión de proteínas recombinantes en hongos filamentosos (1-740 en SEQ ID No. 1), que es el promotor gdhA del gen de la glutamato deshidrogenasa A de Aspergillus niger var. awamori, así como una nueva secuencia de ADN que codifica la glutamato deshidrogenasa (741-2245 en SEQ ID No. 1). Para la expresión de proteínas recombinantes resultan especialmente útiles las construcciones de ADN que comprenden el nuevo promotor, seguido de una secuencia de ADN que codifica una proteína de fusión formada por: un gen que codifique la proteína a obtener (por ejemplo la taumatina), una secuencia espaciadora tipo KEX2, y un gen altamente expresado de un hongo filamentoso. Entre las ventajas de este sistema están: altas concentraciones de proteína expresada, menores tiempos de fermentación, y utilización de una fuente de nitrógeno más económica.
ES9800699A 1998-04-02 1998-04-02 Promotor y construcciones para expresion de proteinas recombinantes en hongos filamentosos. Expired - Fee Related ES2143408B1 (es)

Priority Applications (6)

Application Number Priority Date Filing Date Title
ES9800699A ES2143408B1 (es) 1998-04-02 1998-04-02 Promotor y construcciones para expresion de proteinas recombinantes en hongos filamentosos.
CA002325571A CA2325571A1 (en) 1998-04-02 1999-04-01 Promoter and constructions for expression of recombinant proteins in filamentous fungi
EP99944133A EP1084262A1 (en) 1998-04-02 1999-04-01 Promoter and constructions for expression of recombinant proteins in filamentous fungi
PCT/EP1999/002243 WO1999051756A2 (en) 1998-04-02 1999-04-01 Promoter and constructions for expression of recombinant proteins in filamentous fungi
JP2000542467A JP2002510497A (ja) 1998-04-02 1999-04-01 糸状菌における組換え蛋白質の発現のためのプロモーターおよび組換え構築物
AU42575/99A AU4257599A (en) 1998-04-02 1999-04-01 Promoter and constructions for expression of recombinant proteins in filamentousfungi

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9800699A ES2143408B1 (es) 1998-04-02 1998-04-02 Promotor y construcciones para expresion de proteinas recombinantes en hongos filamentosos.

Publications (2)

Publication Number Publication Date
ES2143408A1 ES2143408A1 (es) 2000-05-01
ES2143408B1 true ES2143408B1 (es) 2000-12-01

Family

ID=8303317

Family Applications (1)

Application Number Title Priority Date Filing Date
ES9800699A Expired - Fee Related ES2143408B1 (es) 1998-04-02 1998-04-02 Promotor y construcciones para expresion de proteinas recombinantes en hongos filamentosos.

Country Status (6)

Country Link
EP (1) EP1084262A1 (es)
JP (1) JP2002510497A (es)
AU (1) AU4257599A (es)
CA (1) CA2325571A1 (es)
ES (1) ES2143408B1 (es)
WO (1) WO1999051756A2 (es)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7883872B2 (en) 1996-10-10 2011-02-08 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
DK1117808T3 (da) 1998-10-06 2005-04-25 Mark Aaron Emalfarb Transformaationssystem inden jfjor området filamentöse svampeværter: I chrysosporium
CN1137265C (zh) * 2000-07-06 2004-02-04 中国科学院微生物研究所 一种提高植物氮素同化效率的方法
EP1231272A3 (en) * 2001-02-08 2002-11-13 Laboratorios Ovejero S.A. Procedure for obtaining bovine chymosin (rennin), curd
WO2003070957A2 (en) * 2002-02-20 2003-08-28 Novozymes A/S Plant polypeptide production
DE102005035888A1 (de) * 2005-07-30 2007-02-01 Maltagen Forschung Gmbh Thaumatin aus transgenen monokotylen Pflanzen
US8680252B2 (en) 2006-12-10 2014-03-25 Dyadic International (Usa), Inc. Expression and high-throughput screening of complex expressed DNA libraries in filamentous fungi
WO2009033071A2 (en) 2007-09-07 2009-03-12 Dyadic International, Inc. Novel fungal enzymes
AU2012298713B2 (en) * 2011-08-24 2017-11-23 Novozymes, Inc. Methods for obtaining positive transformants of a filamentous fungal host cell
BR112014004190A2 (pt) * 2011-08-24 2017-03-01 Novozymes Inc método para construir uma cepa fúngida filamentosa, cepa fúngida filamentosa, método para produzir múltiplos polipeptídeos recombinantes, e, construto em tandem

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI872102A (fi) * 1986-06-06 1987-12-07 Panlabs Inc Expressionssystem hos filamentala fungi.
ES2080689B1 (es) * 1994-04-21 1996-09-01 Urquima Sa Procedimiento de obtencion de un edulcorante natural proteico.
DE69631127T2 (de) * 1995-05-18 2004-07-08 Genencor International, Inc., Palo Alto Glykosyltransferase expression in aspergillus
ES2127697B1 (es) * 1997-03-05 2000-03-16 Antibioticos Sau Promotores de los genes glutamato deshidrogenasa, beta-n-acetilhexosaminidasa y gamma-actina y su utilizacion en sistemas de expresion, secrecion y antisentido de hongos filamentosos.

Also Published As

Publication number Publication date
ES2143408A1 (es) 2000-05-01
EP1084262A1 (en) 2001-03-21
WO1999051756A2 (en) 1999-10-14
AU4257599A (en) 1999-10-25
WO1999051756A9 (en) 2000-02-17
JP2002510497A (ja) 2002-04-09
CA2325571A1 (en) 1999-10-14
WO1999051756A3 (en) 2001-01-18

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