ES2080689B1 - Procedimiento de obtencion de un edulcorante natural proteico. - Google Patents

Procedimiento de obtencion de un edulcorante natural proteico.

Info

Publication number
ES2080689B1
ES2080689B1 ES09400836A ES9400836A ES2080689B1 ES 2080689 B1 ES2080689 B1 ES 2080689B1 ES 09400836 A ES09400836 A ES 09400836A ES 9400836 A ES9400836 A ES 9400836A ES 2080689 B1 ES2080689 B1 ES 2080689B1
Authority
ES
Spain
Prior art keywords
thaumatin
expression
genes
aspergillus niger
artificial
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES09400836A
Other languages
English (en)
Other versions
ES2080689A1 (es
Inventor
Marsal Juan Uriach
Susan Victor Rubio
Martin Cristina Patino
Kalo-Koenova Eliza Iossif
Moral Juarez Catalina Del
Santasusana Igancio Faus
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Urquima SA
Original Assignee
Urquima SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Urquima SA filed Critical Urquima SA
Priority to ES09400836A priority Critical patent/ES2080689B1/es
Priority to FI951868A priority patent/FI112794B/fi
Priority to US08/426,599 priority patent/US5932438A/en
Priority to DK95105973T priority patent/DK0684312T3/da
Priority to CA002147541A priority patent/CA2147541C/en
Priority to ES95105973T priority patent/ES2149901T3/es
Priority to AT95105973T priority patent/ATE194386T1/de
Priority to JP7120674A priority patent/JPH0847396A/ja
Priority to EP95105973A priority patent/EP0684312B1/en
Priority to DE69517750T priority patent/DE69517750T2/de
Priority to PT95105973T priority patent/PT684312E/pt
Publication of ES2080689A1 publication Critical patent/ES2080689A1/es
Application granted granted Critical
Publication of ES2080689B1 publication Critical patent/ES2080689B1/es
Priority to GR20000402190T priority patent/GR3034500T3/el
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C07K14/43—Sweetening agents, e.g. thaumatin, monellin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Botany (AREA)
  • Seasonings (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

PROCEDIMIENTO DE OBTENCION DE UN EDULCORANTE NATURAL PROTEICO. SE POSIBILITA LA OBTENCION DE TAUMATINA II O DE TAUMATINA I MEDIANTE LA EXPRESION, NO DE SECUENCIAS DE SUS GENES NATURALES, SINO DE GENES ARTIFICIALES, SINTETICOS Y SUBSTANCIALMENTE OPTIMIZADOS SIGUIENDO UNAS REGLAS DETERMINADAS. PREFERIBLEMENTE, DICHA EXPRESION SE REALIZA EN HONGOS FILAMENTOSOS, ESPECIALMENTE DEL TIPO GRAS, Y PARTICULARMENTE DE LAS ESPECIES CVAPENICILLIUM ROQUEFORTII, ASPERGILLUS NIGER Y CVAASPERGILLUS NIGER VARIANTE CVAAWAMORI. LA OBTENCION DE GENES ARTIFICIALES SUBSTANCIALMENTE OPTIMIZADOS PARA HONGOS FILAMENTOSOS, REALIZADA AQUI POR PRIMERA VEZ EN EL CASO DE LA TAUMATINA, PERMITE EXPRESIONES ELEVADAS DE PROTEINA, LO QUE HACE QUE EL PROCEDIMIENTO SEA UTIL PARA LA PRODUCCION INDUSTRIAL DE ESTE VALIOSO EDULCORANTE. LAS TAUMATINAS PUEDEN OBTENERSE TANTO EXTRACELULARMENTE, USANDO UN PLASMIDO CON UNA SEÑAL DE EXCRECION, COMO INTRACELULARMENTE, PERMITIENDO ESTO ULTIMO SU UTILIZACION EN ALIMENTACION-ANIMAL SIN SEPARACION PREVIA DEL MICELIO DEL HONGO.
ES09400836A 1994-04-21 1994-04-21 Procedimiento de obtencion de un edulcorante natural proteico. Expired - Fee Related ES2080689B1 (es)

Priority Applications (12)

Application Number Priority Date Filing Date Title
ES09400836A ES2080689B1 (es) 1994-04-21 1994-04-21 Procedimiento de obtencion de un edulcorante natural proteico.
FI951868A FI112794B (fi) 1994-04-21 1995-04-19 Menetelmä luonnollisen proteiinimakeuttamisaineen valmistamiseksi
DE69517750T DE69517750T2 (de) 1994-04-21 1995-04-21 Herstellung eines natürlichen Süssungsmittel-Proteins
CA002147541A CA2147541C (en) 1994-04-21 1995-04-21 Preparation process of a natural protein sweetener
ES95105973T ES2149901T3 (es) 1994-04-21 1995-04-21 Procedimiento de obtencion de un edulcorante natural proteico.
AT95105973T ATE194386T1 (de) 1994-04-21 1995-04-21 Herstellung eines natürlichen süssungsmittel- proteins
US08/426,599 US5932438A (en) 1994-04-21 1995-04-21 Preparation of thaumatin sweeteners
EP95105973A EP0684312B1 (en) 1994-04-21 1995-04-21 Preparation process of a natural protein sweetener
DK95105973T DK0684312T3 (da) 1994-04-21 1995-04-21 Fremgangsmåde til fremstilling af et naturligt sødemiddelprotein
PT95105973T PT684312E (pt) 1994-04-21 1995-04-21 Processo para a preparacao de um edulcorante proteico natural
JP7120674A JPH0847396A (ja) 1994-04-21 1995-04-21 天然タンパク質甘味料の製造方法
GR20000402190T GR3034500T3 (en) 1994-04-21 2000-09-28 Preparation process of a natural protein sweetener.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES09400836A ES2080689B1 (es) 1994-04-21 1994-04-21 Procedimiento de obtencion de un edulcorante natural proteico.

Publications (2)

Publication Number Publication Date
ES2080689A1 ES2080689A1 (es) 1996-02-01
ES2080689B1 true ES2080689B1 (es) 1996-09-01

Family

ID=8285957

Family Applications (2)

Application Number Title Priority Date Filing Date
ES09400836A Expired - Fee Related ES2080689B1 (es) 1994-04-21 1994-04-21 Procedimiento de obtencion de un edulcorante natural proteico.
ES95105973T Expired - Lifetime ES2149901T3 (es) 1994-04-21 1995-04-21 Procedimiento de obtencion de un edulcorante natural proteico.

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES95105973T Expired - Lifetime ES2149901T3 (es) 1994-04-21 1995-04-21 Procedimiento de obtencion de un edulcorante natural proteico.

Country Status (11)

Country Link
US (1) US5932438A (es)
EP (1) EP0684312B1 (es)
JP (1) JPH0847396A (es)
AT (1) ATE194386T1 (es)
CA (1) CA2147541C (es)
DE (1) DE69517750T2 (es)
DK (1) DK0684312T3 (es)
ES (2) ES2080689B1 (es)
FI (1) FI112794B (es)
GR (1) GR3034500T3 (es)
PT (1) PT684312E (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2143408B1 (es) * 1998-04-02 2000-12-01 Urquima Sa Promotor y construcciones para expresion de proteinas recombinantes en hongos filamentosos.
AU2002356880A1 (en) 2001-10-31 2003-05-12 Phytex, Llc Phytase-containing animal food and method
WO2003070957A2 (en) * 2002-02-20 2003-08-28 Novozymes A/S Plant polypeptide production
WO2004050695A2 (en) * 2002-12-05 2004-06-17 Novozymes A/S Expression of polypeptides in filamentous fungi
US20080305522A1 (en) * 2004-01-28 2008-12-11 Keiko Abe Novel Taste-Modifying Polypeptide Nas, Dna Thereof and Use Thereof
CN117835832A (zh) * 2021-08-20 2024-04-05 诺麦生物科学有限公司 包含索马甜和罗汉果苷的甜味组合物

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1192151A (en) * 1980-12-12 1985-08-20 Cornelis T. Verrips Dna sequences encoding the various allelic forms of mature thaumatin, and cloning vehicles comprising said dna's and their use in transformed microorganisms
BR8108073A (pt) * 1980-12-12 1982-09-21 Unilever Nv Sequencias de dna;plasmidios recombinantes;cultura bacteriana;e processo para a producao de preprotaumatina,pretaumatina ou protaumatina
IL68733A (en) * 1982-05-19 1989-01-31 Gist Brocades Nv Kluyveromyces cells transformed by a dna sequence encoding a polypeptide,expression vectors containing said dna sequence and process for the transformation of kluyveromyces cells by said vector
WO1985001746A1 (en) * 1983-10-11 1985-04-25 Beatrice Companies, Inc. The manufacture and expression of genes for thaumatin
DE3650783T2 (de) * 1985-04-15 2004-07-15 Dsm Ip Assets B.V. Verwendung des Glucoamylasepromotors aus Apergillus
CA1310923C (en) * 1985-11-13 1992-12-01 Joachim Ludwig Weickmann Dna encoding [asp --] and [lys -, asp --] thaumatin i
GB2200118A (en) * 1987-01-23 1988-07-27 Allelix Inc Synthetic chymosin-and prochymosin-encoding DNA segments
EP0396612B1 (en) * 1988-01-11 1996-07-24 Xoma Corporation Novel plasmid vector with pectate lyase signal sequence
US5221624A (en) * 1988-11-08 1993-06-22 International Genetic Engineering, Inc. DNA encoding (Lys46, Asp97, Asp113) and (Lys46, Asp.sup.137) thaumatin I polypeptides
JP3986929B2 (ja) * 2002-08-27 2007-10-03 富士通株式会社 電力線搬送通信における漏洩電磁界抑圧送信方法及び漏洩電磁界抑圧送信装置

Also Published As

Publication number Publication date
ES2080689A1 (es) 1996-02-01
FI112794B (fi) 2004-01-15
DE69517750T2 (de) 2001-03-01
CA2147541A1 (en) 1995-10-22
ES2149901T3 (es) 2000-11-16
FI951868A (fi) 1995-10-22
JPH0847396A (ja) 1996-02-20
DE69517750D1 (de) 2000-08-10
PT684312E (pt) 2000-12-29
EP0684312A3 (en) 1996-07-31
EP0684312B1 (en) 2000-07-05
US5932438A (en) 1999-08-03
FI951868A0 (fi) 1995-04-19
EP0684312A2 (en) 1995-11-29
CA2147541C (en) 2000-10-24
ATE194386T1 (de) 2000-07-15
DK0684312T3 (da) 2000-11-13
GR3034500T3 (en) 2000-12-29

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