DE69517750T2 - Herstellung eines natürlichen Süssungsmittel-Proteins - Google Patents

Herstellung eines natürlichen Süssungsmittel-Proteins

Info

Publication number
DE69517750T2
DE69517750T2 DE69517750T DE69517750T DE69517750T2 DE 69517750 T2 DE69517750 T2 DE 69517750T2 DE 69517750 T DE69517750 T DE 69517750T DE 69517750 T DE69517750 T DE 69517750T DE 69517750 T2 DE69517750 T2 DE 69517750T2
Authority
DE
Germany
Prior art keywords
thaumatin
expression
genes
aspergillus niger
artificial
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69517750T
Other languages
English (en)
Other versions
DE69517750D1 (de
Inventor
Juan Uriach-Marsal
Victor Rubio-Susan
Cristina Patino-Martin
Kalo-Koenova Eliza Iossif
Catalina Del-Moral-Juarez
Ignacio Faus-Santasusana
Jose-Luis Del-Rio-Pericacho
Joan Blade-Pique
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Urquima SA
Original Assignee
Urquima SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Urquima SA filed Critical Urquima SA
Application granted granted Critical
Publication of DE69517750D1 publication Critical patent/DE69517750D1/de
Publication of DE69517750T2 publication Critical patent/DE69517750T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C07K14/43—Sweetening agents, e.g. thaumatin, monellin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
DE69517750T 1994-04-21 1995-04-21 Herstellung eines natürlichen Süssungsmittel-Proteins Expired - Fee Related DE69517750T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES09400836A ES2080689B1 (es) 1994-04-21 1994-04-21 Procedimiento de obtencion de un edulcorante natural proteico.

Publications (2)

Publication Number Publication Date
DE69517750D1 DE69517750D1 (de) 2000-08-10
DE69517750T2 true DE69517750T2 (de) 2001-03-01

Family

ID=8285957

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69517750T Expired - Fee Related DE69517750T2 (de) 1994-04-21 1995-04-21 Herstellung eines natürlichen Süssungsmittel-Proteins

Country Status (11)

Country Link
US (1) US5932438A (de)
EP (1) EP0684312B1 (de)
JP (1) JPH0847396A (de)
AT (1) ATE194386T1 (de)
CA (1) CA2147541C (de)
DE (1) DE69517750T2 (de)
DK (1) DK0684312T3 (de)
ES (2) ES2080689B1 (de)
FI (1) FI112794B (de)
GR (1) GR3034500T3 (de)
PT (1) PT684312E (de)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2143408B1 (es) * 1998-04-02 2000-12-01 Urquima Sa Promotor y construcciones para expresion de proteinas recombinantes en hongos filamentosos.
BRPI0213813B1 (pt) 2001-10-31 2018-11-21 Cornell Res Foundation Inc método para aprimorar o valor nutricional de uma ração consumida por um animal monogástrico e ração
WO2003070957A2 (en) * 2002-02-20 2003-08-28 Novozymes A/S Plant polypeptide production
WO2004050695A2 (en) * 2002-12-05 2004-06-17 Novozymes A/S Expression of polypeptides in filamentous fungi
WO2005073372A1 (ja) * 2004-01-28 2005-08-11 Mitsukan Group Corporation 新規味覚改変ポリペプチドnas、そのdna及びその用途
WO2023021220A1 (en) * 2021-08-20 2023-02-23 Nomad Bioscience Gmbh Sweetening composition comprising thaumatin and mogrosides

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3175357D1 (en) * 1980-12-12 1986-10-23 Unilever Nv Dna sequences encoding various allelic forms of mature thaumatin, recombinant plasmids comprising said dna's and a process for their preparation, bacterial cultures comprising said recombinant plasmids, and method for producing mature thaumatin
EP0054331B1 (de) * 1980-12-12 1992-06-03 Unilever N.V. Strukturgene, die für die verschiedenen allelischen und Reifungsformen von Präprothaumatin und deren Mutanten kodieren, diese Strukturgene enthaltende Klonierungsvektoren und deren Expression in mikrobiellen Wirtzellen
HU204097B (en) * 1982-05-19 1991-11-28 Gist Brocades Nv Process for producing cloning system relating to kluyveromyces species
JPS61501186A (ja) * 1983-10-11 1986-06-19 インターナシヨナル　ジネテイツク　エンジニアリング　インコーポレイテツド タウマチン（Ｔｈａｕｍａｔｉｎ）遺伝子の作製と発現
AU606033B2 (en) * 1985-04-15 1991-01-31 Dsm Ip Assets B.V. Promotors of filamentous fungi and use thereof
CA1310923C (en) * 1985-11-13 1992-12-01 Joachim Ludwig Weickmann Dna encoding [asp --] and [lys -, asp --] thaumatin i
GB2200118A (en) * 1987-01-23 1988-07-27 Allelix Inc Synthetic chymosin-and prochymosin-encoding DNA segments
ATE140731T1 (de) * 1988-01-11 1996-08-15 Xoma Corp Plasmidvektor mit pectatlyase-signalsequenz
US5221624A (en) * 1988-11-08 1993-06-22 International Genetic Engineering, Inc. DNA encoding (Lys46, Asp97, Asp113) and (Lys46, Asp.sup.137) thaumatin I polypeptides
JP3986929B2 (ja) * 2002-08-27 2007-10-03 富士通株式会社 電力線搬送通信における漏洩電磁界抑圧送信方法及び漏洩電磁界抑圧送信装置

Also Published As

Publication number Publication date
US5932438A (en) 1999-08-03
PT684312E (pt) 2000-12-29
FI951868A0 (fi) 1995-04-19
EP0684312B1 (de) 2000-07-05
CA2147541C (en) 2000-10-24
ES2080689A1 (es) 1996-02-01
EP0684312A2 (de) 1995-11-29
EP0684312A3 (de) 1996-07-31
JPH0847396A (ja) 1996-02-20
CA2147541A1 (en) 1995-10-22
GR3034500T3 (en) 2000-12-29
DE69517750D1 (de) 2000-08-10
ES2080689B1 (es) 1996-09-01
FI112794B (fi) 2004-01-15
DK0684312T3 (da) 2000-11-13
ES2149901T3 (es) 2000-11-16
ATE194386T1 (de) 2000-07-15
FI951868A (fi) 1995-10-22

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee