DE69517750D1 - Herstellung eines natürlichen Süssungsmittel-Proteins - Google Patents

Herstellung eines natürlichen Süssungsmittel-Proteins

Info

Publication number
DE69517750D1
DE69517750D1 DE69517750T DE69517750T DE69517750D1 DE 69517750 D1 DE69517750 D1 DE 69517750D1 DE 69517750 T DE69517750 T DE 69517750T DE 69517750 T DE69517750 T DE 69517750T DE 69517750 D1 DE69517750 D1 DE 69517750D1
Authority
DE
Germany
Prior art keywords
thaumatin
expression
genes
aspergillus niger
artificial
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69517750T
Other languages
English (en)
Other versions
DE69517750T2 (de
Inventor
Juan Uriach-Marsal
Victor Rubio-Susan
Cristina Patino-Martin
Kalo-Koenova Eliza Iossif
Catalina Del-Moral-Juarez
Ignacio Faus-Santasusana
Jose-Luis Del-Rio-Pericacho
Joan Blade-Pique
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Urquima SA
Original Assignee
Urquima SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Urquima SA filed Critical Urquima SA
Application granted granted Critical
Publication of DE69517750D1 publication Critical patent/DE69517750D1/de
Publication of DE69517750T2 publication Critical patent/DE69517750T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C07K14/43—Sweetening agents, e.g. thaumatin, monellin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Seasonings (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
DE69517750T 1994-04-21 1995-04-21 Herstellung eines natürlichen Süssungsmittel-Proteins Expired - Fee Related DE69517750T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES09400836A ES2080689B1 (es) 1994-04-21 1994-04-21 Procedimiento de obtencion de un edulcorante natural proteico.

Publications (2)

Publication Number Publication Date
DE69517750D1 true DE69517750D1 (de) 2000-08-10
DE69517750T2 DE69517750T2 (de) 2001-03-01

Family

ID=8285957

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69517750T Expired - Fee Related DE69517750T2 (de) 1994-04-21 1995-04-21 Herstellung eines natürlichen Süssungsmittel-Proteins

Country Status (11)

Country Link
US (1) US5932438A (de)
EP (1) EP0684312B1 (de)
JP (1) JPH0847396A (de)
AT (1) ATE194386T1 (de)
CA (1) CA2147541C (de)
DE (1) DE69517750T2 (de)
DK (1) DK0684312T3 (de)
ES (2) ES2080689B1 (de)
FI (1) FI112794B (de)
GR (1) GR3034500T3 (de)
PT (1) PT684312E (de)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2143408B1 (es) * 1998-04-02 2000-12-01 Urquima Sa Promotor y construcciones para expresion de proteinas recombinantes en hongos filamentosos.
ES2597503T3 (es) 2001-10-31 2017-01-19 Huvepharma Eood Alimento para animales que contiene fitasa y método
AU2003206684A1 (en) * 2002-02-20 2003-09-09 Novozymes A/S Plant polypeptide production
AU2003286128A1 (en) * 2002-12-05 2004-06-23 Novozymes A/S Expression of polypeptides in filamentous fungi
US20080305522A1 (en) * 2004-01-28 2008-12-11 Keiko Abe Novel Taste-Modifying Polypeptide Nas, Dna Thereof and Use Thereof
WO2023021220A1 (en) * 2021-08-20 2023-02-23 Nomad Bioscience Gmbh Sweetening composition comprising thaumatin and mogrosides

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IE52417B1 (en) * 1980-12-12 1987-10-28 Unilever Plc Dna sequences encoding various allelic forms of mature thaumatin,recombinant plasmids comprising said dnas and a process for their preparation,bacterial cultures comprising said recombinant plasmids,and method for producing mature thaumatin
EP0054331B1 (de) * 1980-12-12 1992-06-03 Unilever N.V. Strukturgene, die für die verschiedenen allelischen und Reifungsformen von Präprothaumatin und deren Mutanten kodieren, diese Strukturgene enthaltende Klonierungsvektoren und deren Expression in mikrobiellen Wirtzellen
AR241796A1 (es) * 1982-05-19 1992-12-30 Gist Brocades Nv Procedimiento para preparar levadura klyveromyces que contienen vectores que regulan la expresion de proteina y/o capaces de la expresion de proteinas homologas o heterologas.
WO1985001746A1 (en) * 1983-10-11 1985-04-25 Beatrice Companies, Inc. The manufacture and expression of genes for thaumatin
ATE161881T1 (de) * 1985-04-15 1998-01-15 Gist Brocades Nv Verwendung von promotoren von filamentösen pilzen
CA1310923C (en) * 1985-11-13 1992-12-01 Joachim Ludwig Weickmann Dna encoding [asp --] and [lys -, asp --] thaumatin i
GB2200118A (en) * 1987-01-23 1988-07-27 Allelix Inc Synthetic chymosin-and prochymosin-encoding DNA segments
DE68926882T2 (de) * 1988-01-11 1997-02-13 Xoma Corp Plasmidvektor mit pectatlyase-signalsequenz
US5221624A (en) * 1988-11-08 1993-06-22 International Genetic Engineering, Inc. DNA encoding (Lys46, Asp97, Asp113) and (Lys46, Asp.sup.137) thaumatin I polypeptides
JP3986929B2 (ja) * 2002-08-27 2007-10-03 富士通株式会社 電力線搬送通信における漏洩電磁界抑圧送信方法及び漏洩電磁界抑圧送信装置

Also Published As

Publication number Publication date
FI951868A (fi) 1995-10-22
ATE194386T1 (de) 2000-07-15
EP0684312B1 (de) 2000-07-05
CA2147541A1 (en) 1995-10-22
CA2147541C (en) 2000-10-24
ES2149901T3 (es) 2000-11-16
DE69517750T2 (de) 2001-03-01
GR3034500T3 (en) 2000-12-29
DK0684312T3 (da) 2000-11-13
FI951868A0 (fi) 1995-04-19
FI112794B (fi) 2004-01-15
PT684312E (pt) 2000-12-29
ES2080689A1 (es) 1996-02-01
EP0684312A2 (de) 1995-11-29
ES2080689B1 (es) 1996-09-01
EP0684312A3 (de) 1996-07-31
JPH0847396A (ja) 1996-02-20
US5932438A (en) 1999-08-03

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee