DK1117808T3 - Transformaationssystem inden jfjor området filamentöse svampeværter: I chrysosporium - Google Patents

Transformaationssystem inden jfjor området filamentöse svampeværter: I chrysosporium

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Publication number
DK1117808T3
DK1117808T3 DK99949459T DK99949459T DK1117808T3 DK 1117808 T3 DK1117808 T3 DK 1117808T3 DK 99949459 T DK99949459 T DK 99949459T DK 99949459 T DK99949459 T DK 99949459T DK 1117808 T3 DK1117808 T3 DK 1117808T3
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Denmark
Prior art keywords
chrysosporium
filamentous fungal
transformational
sequences
area
Prior art date
Application number
DK99949459T
Other languages
English (en)
Inventor
Richard Paul Burlingame
Philip Terry Olson
Arkady Panteleimonovi Sinitsyn
Martine Parriche
Jean Christophe Bousson
Christine Marie Pynnonen
Peter Jan Punt
Zeijl Cornelia Maria Johan Van
Mark Aaron Emalfarb
Original Assignee
Mark Aaron Emalfarb
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Mark Aaron Emalfarb filed Critical Mark Aaron Emalfarb
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Publication of DK1117808T3 publication Critical patent/DK1117808T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2462—Lysozyme (3.2.1.17)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C12N9/2482—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Radio Relay Systems (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
DK99949459T 1998-10-06 1999-10-06 Transformaationssystem inden jfjor området filamentöse svampeværter: I chrysosporium DK1117808T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EPPCT/EP98/06496 1998-10-06
EP99949459A EP1117808B1 (en) 1998-10-06 1999-10-06 Transformation system in the field of filamentous fungal hosts: in chrysosporium

Publications (1)

Publication Number Publication Date
DK1117808T3 true DK1117808T3 (da) 2005-04-25

Family

ID=8167095

Family Applications (1)

Application Number Title Priority Date Filing Date
DK99949459T DK1117808T3 (da) 1998-10-06 1999-10-06 Transformaationssystem inden jfjor området filamentöse svampeværter: I chrysosporium

Country Status (13)

Country Link
US (4) US6573086B1 (da)
EP (1) EP1117808B1 (da)
KR (1) KR100618495B1 (da)
CN (1) CN1230546C (da)
AT (1) ATE286136T1 (da)
AU (1) AU771539C (da)
BR (1) BRPI9914278B1 (da)
CA (1) CA2345356C (da)
DE (1) DE69922978T2 (da)
DK (1) DK1117808T3 (da)
EA (1) EA005682B1 (da)
ES (1) ES2237159T3 (da)
WO (1) WO2000020555A2 (da)

Families Citing this family (99)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7883872B2 (en) 1996-10-10 2011-02-08 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
KR100618495B1 (ko) * 1998-10-06 2006-08-31 마크 아론 에말파브 섬유상의 진균성 숙주 영역에서의 형질전환 시스템:크리소스포륨속에서
DK1123974T3 (da) * 1998-10-23 2010-08-23 Meiji Seika Kaisha Endoglucanaser og cellulasefremstillinger indeholdende dem
KR100312070B1 (ko) * 1999-03-15 2001-11-03 박호군 고속 선별법을 포함하는 개선된 형질을 갖는 효소 변이체의 제조방법
CZ303980B6 (cs) 2000-04-13 2013-07-31 Dyadic International (Usa), Inc. Expresi regulující sekvence nukleové kyseliny a produkty exprese v oblasti vláknitých plísní
KR20020026456A (ko) 2000-04-13 2002-04-10 에말파브 마크 아론 사상균에서 발현된 ｄｎａ 라이브러리의 고산출량 스크리닝
EP1291431B1 (en) * 2000-05-22 2006-12-27 Meiji Seika Kaisha Ltd. Endoglucanase nce5
EP2292271A3 (en) 2001-10-10 2011-09-14 BioGeneriX AG Remodelling and glycoconjugation of an antibody
WO2004099231A2 (en) 2003-04-09 2004-11-18 Neose Technologies, Inc. Glycopegylation methods and proteins/peptides produced by the methods
EP2322229B1 (en) 2001-10-10 2016-12-21 Novo Nordisk A/S Remodeling and glycoconjugation of Factor IX
FI118339B (fi) * 2004-09-21 2007-10-15 Ab Enzymes Oy Uusi lakkaasientsyymi ja sen käyttö
US8008056B2 (en) * 2004-12-30 2011-08-30 Danisco Us Inc. Variant Hypocrea jecorina CBH2 cellulases
WO2007071820A1 (en) * 2005-12-22 2007-06-28 Ab Enzymes Oy Novel enzymes
US7256032B2 (en) 2005-12-22 2007-08-14 Ab Enzymes Oy Enzymes
FI119325B (fi) * 2005-12-22 2008-10-15 Ab Enzymes Oy Uusia endoglukanaasi polypeptidejä ja niiden tuotto ja käyttö
CN103740674A (zh) 2006-03-20 2014-04-23 诺维信股份有限公司 具有内切葡聚糖酶活性的多肽和编码该多肽的多核苷酸
US20110104750A1 (en) * 2006-04-05 2011-05-05 Michael Ward Filamentous Fungi Having Reduced UDP-Galactofuranose Content
US8304212B2 (en) * 2006-07-10 2012-11-06 Dyadic International, Inc. Methods and compositions for degradation of lignocellulosic material
US20080026376A1 (en) 2006-07-11 2008-01-31 Huaming Wang KEX2 cleavage regions of recombinant fusion proteins
EP2102366A4 (en) 2006-12-10 2010-01-27 Dyadic International Inc EXPRESSION AND HIGH-DROP SCREENING OF COMPLEX EXPRESSED DNA LIBRARIES IN FADENED MUSHROOMS
US9862956B2 (en) 2006-12-10 2018-01-09 Danisco Us Inc. Expression and high-throughput screening of complex expressed DNA libraries in filamentous fungi
EP2069491B1 (en) 2007-05-10 2018-01-03 Novozymes Inc. Compositions and methods for enhancing the degradation or conversion of cellulose-containing material
US7923236B2 (en) * 2007-08-02 2011-04-12 Dyadic International (Usa), Inc. Fungal enzymes
WO2009033071A2 (en) 2007-09-07 2009-03-12 Dyadic International, Inc. Novel fungal enzymes
WO2010107303A2 (en) 2009-03-16 2010-09-23 Dyadic Nederland B.V. New fungal production system
BRPI1010239A2 (pt) 2009-03-31 2016-10-11 Codexis Inc endoglucanases aprimoradas, derivados e seus usos
WO2010129287A2 (en) 2009-04-27 2010-11-11 The Board Of Trustees Of The University Of Illinois Hemicellulose-degrading enzymes
FI121712B (fi) 2009-04-30 2011-03-15 Ab Enzymes Oy Uusi sieniperäinen proteaasi ja sen käyttö
FI121711B (fi) * 2009-04-30 2011-03-15 Ab Enzymes Oy Sieniperäinen seriiniproteaasi ja sen käyttö
WO2010148148A2 (en) 2009-06-16 2010-12-23 Codexis, Inc. β-GLUCOSIDASE VARIANTS
FI121851B (fi) 2009-07-08 2011-05-13 Ab Enzymes Oy Sieniperäinen proteaasi ja sen käyttö
DK2480660T5 (da) 2009-09-23 2020-11-09 Danisco Us Inc Hidtil ukendte glycosylhydrolaseenzymer og anvendelser heraf
WO2011041594A1 (en) 2009-09-30 2011-04-07 Codexis, Inc. Recombinant c1 b-glucosidase for production of sugars from cellulosic biomass
CN102666848B (zh) 2009-11-25 2014-09-24 科德克希思公司 用于从纤维素生物质产生可发酵的糖的重组嗜热子囊菌β-葡糖苷酶变体
MX355841B (es) 2009-11-25 2018-05-02 Codexis Inc Variantes de beta-glucosidasa recombinante para producción de azúcares solubles de biomasa celulósica.
DE102009055015B4 (de) * 2009-12-18 2013-12-24 Technische Universität Dresden Hefe-Sekretionssystem auf Basis einer Signalsequenz aus der Hydrophobin 1-Gensequenz von Trichoderma reesei
MX2012007224A (es) 2009-12-23 2012-07-30 Danisco Us Inc Metodos para mejorar la eficacia de las reacciones de sacarificacion y fermentacion simultaneas.
FI122937B (fi) 2009-12-30 2012-09-14 Roal Oy Menetelmä selluloosamateriaalin käsittelemiseksi sekä tässä käyttökelpoiset CBH II/Cel6A entsyymit
US9322027B2 (en) 2010-08-20 2016-04-26 Shell Oil Company Expression constructs comprising fungal promoters
CA2807702C (en) 2010-08-20 2018-07-24 Codexis, Inc. Use of glycoside hydrolase 61 family proteins in processing of cellulose
AU2011317171B2 (en) * 2010-10-20 2014-09-25 Danisco Us Inc. Thermostable Trichoderma cellulase
FI123942B (fi) 2010-10-29 2013-12-31 Ab Enzymes Oy Sieniperäisen seriiniproteaasin variantteja
US9068235B2 (en) 2010-11-02 2015-06-30 Codexis, Inc. Fungal strains
CN103189516A (zh) 2010-11-02 2013-07-03 科德克希思公司 用于产生可发酵糖类的组合物和方法
WO2012068236A2 (en) * 2010-11-16 2012-05-24 Dyadic International (Usa) Inc. Novel fungal oxidoreductases
CN102154237A (zh) * 2011-01-04 2011-08-17 深圳市绿微康生物工程有限公司 一种有效降低动物体内胆固醇、血脂水平的脂肪酶及其应用
CN102154358B (zh) * 2011-01-04 2013-07-10 深圳市绿微康生物工程有限公司 一种高效表达的产脂肪酶基因质粒的构建方法
CN102154238A (zh) * 2011-01-04 2011-08-17 深圳市绿微康生物工程有限公司 一种有效降低人体胆固醇、血脂水平的脂肪酶及其应用
CN102154340B (zh) * 2011-01-04 2013-07-10 深圳市绿微康生物工程有限公司 一种获得高效表达脂肪酶菌株的方法
CA2830508A1 (en) 2011-03-17 2012-09-20 Danisco Us Inc. Method for reducing viscosity in saccharification process
WO2012125951A1 (en) 2011-03-17 2012-09-20 Danisco Us Inc Cellulase compositions and methods of using the same for improved conversion of lignocellulosic biomass into fermentable sugars
FI123425B (fi) 2011-03-31 2013-04-30 Ab Enzymes Oy Proteaasientsyymi ja sen käytöt
CN103014036A (zh) * 2011-07-01 2013-04-03 深圳市绿微康生物工程有限公司 改型扩展青霉脂肪酶基因及其构建和表达方法
BR112014004171A2 (pt) 2011-08-22 2018-11-06 Codexis Inc variantes da proteína glicósideo hidrolase gh61 e cofatores que aumentam a atividade de gh61
WO2013048661A1 (en) 2011-09-30 2013-04-04 Codexis, Inc. Fungal proteases
WO2013067028A1 (en) 2011-10-31 2013-05-10 Bp Corporation North America Inc. Use of mammalian promoters in filamentous fungi
WO2013067026A1 (en) 2011-10-31 2013-05-10 Bp Corporation North America Inc. Use of plant promoters in filamentous fungi
US20140234897A1 (en) * 2011-11-04 2014-08-21 Novozymes Inc. Polypeptides Having Cellobiohydrolase Activity and Polynucleotides Encoding Same
BR112014015030A2 (pt) 2011-12-20 2017-06-13 Codexis Inc variantes da endoflucanase 1b (eg1b)
BR112014031024A2 (pt) 2012-06-11 2017-06-27 Codexis Inc xilanases e xilosidases fúngicas.
WO2014070856A2 (en) 2012-11-02 2014-05-08 Bp Corporation North America Inc. Thermotolerant beta-glucosidase variants
WO2014145768A2 (en) 2013-03-15 2014-09-18 Bp Corporation North America Inc. Use of non-fungal 5' utrs in filamentous fungi
ES2521940B1 (es) 2013-05-10 2015-09-09 Abengoa Bioenergía Nuevas Tecnologías, S.A. Variantes de beta-glucosidasa con actividad de transglicosilación reducida
BR112015029081A2 (pt) 2013-05-20 2017-09-19 Abengoa Bioenergia Nuevas Tecnologias Sa Célula hospedeira myceliophthora thermophila expressando uma enzima beta-xilosidase recombinante, enzima beta-xilosidase recombinante expressada por meio da mesma, composição, uso de célula hospedeira, enzima beta-xilosidase ou composição e métodos de produção de açúcares fermentescíveis e de produção de bioproduto a partir de biomassa
ES2527368B1 (es) 2013-07-22 2015-10-27 Abengoa Bioenergía Nuevas Tecnologías, S.A. Célula hospedadora de Myceliophthora thermophila que expresa una enzima alfa-xilosidasa heteróloga y su uso en un procedimiento de degradación de biomasa
EP3074513A1 (en) 2013-11-26 2016-10-05 Novozymes A/S Enzyme compositions and uses thereof
ES2542621B1 (es) 2014-02-07 2016-08-16 Abengoa Bioenergía Nuevas Tecnologías, S.A. Polipéptidos con actividad polisacárido monooxigenasa y su uso para la producción de azúcares fermentables
EP3152315B1 (en) 2014-06-06 2018-08-15 Novozymes A/S Enzyme compositions and uses thereof
WO2016142536A1 (en) 2015-03-11 2016-09-15 Genencor International B.V. Enzymatic activity of lytic polysaccharide monooxygenase
KR102604503B1 (ko) 2015-05-07 2023-11-20 코덱시스, 인코포레이티드 페니실린-g 아실라아제
EP3313999B1 (en) 2015-06-24 2021-04-28 Genencor International B.V. Polypeptides having demethylating activity
EP3330375A1 (en) 2015-07-29 2018-06-06 Abengoa Bioenergía Nuevas Tecnologías, S. A. Expression of recombinant beta-xylosidase enzymes
CN108291191A (zh) 2015-12-02 2018-07-17 巴斯夫欧洲公司 在具有降低的clr1活性的丝状真菌中生产蛋白质的方法
KR20180084135A (ko) 2015-12-02 2018-07-24 바스프 에스이 감소된 clr2 활성을 갖는 사상 진균에서 단백질을 생산하는 방법
US11208649B2 (en) 2015-12-07 2021-12-28 Zymergen Inc. HTP genomic engineering platform
US9988624B2 (en) 2015-12-07 2018-06-05 Zymergen Inc. Microbial strain improvement by a HTP genomic engineering platform
KR20180084756A (ko) 2015-12-07 2018-07-25 지머젠 인코포레이티드 코리네박테리움 글루타미컴으로부터의 프로모터
ES2855728T3 (es) 2016-02-22 2021-09-24 Danisco Us Inc Sistema fúngico de producción de proteínas de alto nivel
JP7128519B2 (ja) 2016-05-05 2022-08-31 コデクシス， インコーポレイテッド ペニシリンｇアシラーゼ
ES2647322B1 (es) 2016-05-20 2018-10-05 Abengoa Bioenergía Nuevas Tecnologías, S.A. Célula hospedadora Myceliophthora thermophila con actividad celulolítica mejorada y composiciones enzimáticas obtenidas por la misma
JP2019519241A (ja) 2016-06-30 2019-07-11 ザイマージェン インコーポレイテッド グルコース透過酵素ライブラリーを生成するための方法およびその使用
RU2019102378A (ru) 2016-06-30 2020-07-30 ДАНИСКО ЮЭс ИНК. Аспарагиновые протеазы
JP2019519242A (ja) 2016-06-30 2019-07-11 ザイマージェン インコーポレイテッド 細菌ヘモグロビンライブラリーを生成するための方法およびその使用
EP3565893A4 (en) 2017-01-05 2020-12-09 Codexis, Inc. PENICILLIN G ACYLASES
EP3378936B1 (en) * 2017-03-24 2021-05-12 Clariant International Ltd Cellulase suitable for use in detergent compositions
CN110719956A (zh) 2017-06-06 2020-01-21 齐默尔根公司 用于改良真菌菌株的高通量基因组工程改造平台
CA3064574A1 (en) 2017-06-14 2018-12-20 Codexis, Inc. Engineered transaminase polypeptides for industrial biocatalysis
US11643642B2 (en) 2017-06-27 2023-05-09 Codexis, Inc. Penicillin-g acylases
CA3073646A1 (en) * 2017-08-21 2019-02-28 Dyadic International Inc. Production of flu vaccine in myceliophthora thermophila
US11319531B2 (en) 2017-11-07 2022-05-03 Codexis, Inc. Transglutaminase variants
CN109988715B (zh) * 2017-12-29 2022-03-01 青岛蔚蓝生物集团有限公司 一种高产木聚糖酶的突变菌株及其应用
CN110093332B (zh) * 2018-01-30 2021-12-28 青岛蔚蓝生物集团有限公司 一种纤维素酶突变体及其高产菌株
WO2019236848A1 (en) 2018-06-06 2019-12-12 Zymergen Inc. Manipulation of genes involved in signal transduction to control fungal morphology during fermentation and production
CA3103721A1 (en) 2018-07-09 2020-01-16 Codexis, Inc. Engineered deoxyribose-phosphate aldolases
CN108913713A (zh) * 2018-07-24 2018-11-30 中国农业科学院饲料研究所 提高里氏木霉转化效率的重组表达载体及应用
EP4133064A2 (en) 2020-04-10 2023-02-15 Codexis, Inc. Engineered transaminase polypeptides
WO2021220209A1 (en) 2020-04-30 2021-11-04 Dyadic International (Usa), Inc. Nicotinamide riboside production in filamentous fungi
US11479779B2 (en) * 2020-07-31 2022-10-25 Zymergen Inc. Systems and methods for high-throughput automated strain generation for non-sporulating fungi
CN113403209B (zh) * 2021-07-30 2022-08-26 西南大学 天冬氨酸蛋白酶基因在改良球孢白僵菌菌株中的应用

Family Cites Families (111)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2974001A (en) * 1957-07-20 1961-03-07 Kalle Ag Process and compositions for enzymatic desizing and bleaching of textiles
GB1368599A (en) 1970-09-29 1974-10-02 Unilever Ltd Softening compositions
JPS5028515B2 (da) * 1971-09-30 1975-09-16
US3966543A (en) * 1972-10-30 1976-06-29 Baxter Laboratories, Inc. Enzyme-treated paper
US4081328A (en) * 1975-10-23 1978-03-28 Stanford Research Institute Production of cellulase by a thermophilic thielavia terrestris
DK187280A (da) * 1980-04-30 1981-10-31 Novo Industri As Ruhedsreducerende middel til et fuldvaskemiddel fuldvaskemiddel og fuldvaskemetode
GB2094826B (en) 1981-03-05 1985-06-12 Kao Corp Cellulase enzyme detergent composition
JPS591598A (ja) * 1982-06-25 1984-01-06 花王株式会社 洗浄剤組成物
US4486533A (en) * 1982-09-02 1984-12-04 St. Louis University Filamentous fungi functional replicating extrachromosomal element
NL8300698A (nl) * 1983-02-24 1984-09-17 Univ Leiden Werkwijze voor het inbouwen van vreemd dna in het genoom van tweezaadlobbige planten; agrobacterium tumefaciens bacterien en werkwijze voor het produceren daarvan; planten en plantecellen met gewijzigde genetische eigenschappen; werkwijze voor het bereiden van chemische en/of farmaceutische produkten.
GB8306645D0 (en) * 1983-03-10 1983-04-13 Unilever Plc Detergent compositions
US4462307A (en) * 1983-05-23 1984-07-31 Pet Incorporated Humpback oven-broiler
GB8421800D0 (en) * 1984-08-29 1984-10-03 Unilever Plc Detergent compositions
GB8422238D0 (en) 1984-09-03 1984-10-10 Neuberger M S Chimeric proteins
US4816405A (en) * 1984-10-24 1989-03-28 The Regents Of The University Of California Vectors for transformation by ascomycetes
US4885249A (en) * 1984-12-05 1989-12-05 Allelix, Inc. Aspergillus niger transformation system
US4610800A (en) * 1985-01-25 1986-09-09 Genex Corporation Method for unclogging drainage pipes
US6492107B1 (en) * 1986-11-20 2002-12-10 Stuart Kauffman Process for obtaining DNA, RNA, peptides, polypeptides, or protein, by recombinant DNA technique
US5198345A (en) * 1985-04-15 1993-03-30 Gist-Brocades N.V. Vectors in use in filamentous fungi
US5364770A (en) * 1985-08-29 1994-11-15 Genencor International Inc. Heterologous polypeptides expressed in aspergillus
AU607398B2 (en) * 1985-08-29 1991-03-07 Genencor Inc. Heterologous polypeptides expressed in filamentous fungi, processes for making same, and vectors for making same
DK163591C (da) 1985-10-08 1992-08-24 Novo Nordisk As Fremgangsmaade til behandling af et tekstilstof med en cellulase
US4935349A (en) * 1986-01-17 1990-06-19 Zymogenetics, Inc. Expression of higher eucaryotic genes in aspergillus
US5536661A (en) * 1987-03-10 1996-07-16 Novo Nordisk A/S Process for the production of protein products in aspergillus
US5766912A (en) * 1986-03-17 1998-06-16 Novo Nordisk A/S Humicola lipase produced in aspergillus
GB8607679D0 (en) 1986-03-27 1986-04-30 Winter G P Recombinant dna product
US5252726A (en) * 1987-09-04 1993-10-12 Novo Nordisk A/S Promoters for use in aspergillus
US4832864A (en) * 1987-09-15 1989-05-23 Ecolab Inc. Compositions and methods that introduce variations in color density into cellulosic fabrics, particularly indigo dyed denim
US5223409A (en) * 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
US5122159A (en) * 1988-09-15 1992-06-16 Ecolab Inc. Cellulase compositions and methods that introduce variations in color density into cellulosic fabrics, particularly indigo dyed denim
US5006126A (en) * 1988-09-15 1991-04-09 Ecolab Inc. Cellulase compositions and methods that introduce variations in color density into cellulosic fabrics, particularly indigo dyed denim
IL162181A (en) 1988-12-28 2006-04-10 Pdl Biopharma Inc A method of producing humanized immunoglubulin, and polynucleotides encoding the same
US5571697A (en) * 1989-05-05 1996-11-05 Baylor College Of Medicine Texas Medical Center Expression of processed recombinant lactoferrin and lactoferrin polypeptide fragments from a fusion product in Aspergillus
JPH05503919A (ja) 1989-06-13 1993-06-24 スミスクライン・ビーチャム・コーポレイション 単球および／またはマクロファージによるインターロイキン―１または腫瘍壊死因子生成の抑制
EP0481008B1 (en) 1989-07-07 1997-11-12 Unilever Plc Process for preparing a protein by a fungus transformed by multicopy integration of an expression vector
US5120463A (en) * 1989-10-19 1992-06-09 Genencor International, Inc. Degradation resistant detergent compositions based on cellulase enzymes
GB8928874D0 (en) 1989-12-21 1990-02-28 Celltech Ltd Humanised antibodies
WO1991013971A1 (en) * 1990-03-13 1991-09-19 Hawaii Biotechnology Group, Inc. Neurospora expression system
DK115890D0 (da) * 1990-05-09 1990-05-09 Novo Nordisk As Enzym
GB9015198D0 (en) * 1990-07-10 1990-08-29 Brien Caroline J O Binding substance
WO1992001069A1 (en) * 1990-07-13 1992-01-23 Transkaryotic Therapies, Inc. Library screening method
CA2090126C (en) * 1990-08-02 2002-10-22 John W. Schrader Methods for the production of proteins with a desired function
JP3133774B2 (ja) * 1990-10-04 2001-02-13 日本食品化工株式会社 新規な宿主−ベクター系
US5290474A (en) * 1990-10-05 1994-03-01 Genencor International, Inc. Detergent composition for treating cotton-containing fabrics containing a surfactant and a cellulase composition containing endolucanase III from trichoderma ssp
US5780279A (en) * 1990-12-03 1998-07-14 Genentech, Inc. Method of selection of proteolytic cleavage sites by directed evolution and phagemid display
ES2182818T5 (es) * 1990-12-10 2015-07-06 Danisco Us Inc. Sacarificación mejorada de celulosa por clonación y amplificación del gen de beta-glucosidasa de Trichoderma reesei
GB9102635D0 (en) 1991-02-07 1991-03-27 British Bio Technology Compounds
DE69230142T2 (de) * 1991-05-15 2000-03-09 Cambridge Antibody Tech Verfahren zur herstellung von spezifischen bindungspaargliedern
US5858657A (en) * 1992-05-15 1999-01-12 Medical Research Council Methods for producing members of specific binding pairs
ES2136092T3 (es) * 1991-09-23 1999-11-16 Medical Res Council Procedimientos para la produccion de anticuerpos humanizados.
US5516670A (en) * 1991-09-30 1996-05-14 Kuehnle; Adelheid R. Magnetophoretic particle delivery method and apparatus for the treatment of cells
BE1005432A4 (nl) 1991-10-01 1993-07-20 Dsm Nv Expressiecassette voor heterologe eiwitten.
DK0672149T3 (da) * 1991-12-09 2001-11-05 Unilever Nv Fremgangsmåde til fremstilling/udskilning af et protein med en transformeret skimmelsvamp ved anvendelse af ekspressions/udskilningsregulerende regioner afledt af et Aspergillus-endoxylanase II-gen
CA2062023A1 (en) * 1992-02-10 1993-08-11 Jagroop S. Dahiya Novel fungal strains and use thereof in antibiotic production
WO1994004673A1 (en) 1992-08-19 1994-03-03 Alko Group Ltd. Fungal promoters active in the presence of glucose
WO1994005781A1 (en) * 1992-09-04 1994-03-17 The Scripps Research Institute Phagemids coexpressing a surface receptor and a surface heterologous protein
WO1994013820A1 (en) 1992-12-10 1994-06-23 Gist-Brocades N.V. Production of heterologous proteins in filamentous fungi
GB9316883D0 (en) * 1993-08-13 1993-09-29 Univ Leeds Production of heterologous peptices
EP0726940A4 (en) * 1993-11-02 2001-05-23 Merck & Co Inc DNA ENCODING A TRIOL-POLYCETIDE-SYNTHASE
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5820866A (en) * 1994-03-04 1998-10-13 National Jewish Center For Immunology And Respiratory Medicine Product and process for T cell regulation
GB2289218A (en) 1994-05-06 1995-11-15 Merck & Co Inc Inhibition of TNFalpha production with agonists of the A2b subtype of the adenosine receptor
AU2733895A (en) * 1994-06-17 1996-01-15 Novo Nordisk A/S A fungus wherein the area gene has been modified and an area gene from aspergillus oryzae
US6060305A (en) * 1994-06-30 2000-05-09 Novo Nordisk Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic Fusarium expression system
EP1559776A3 (en) * 1994-06-30 2006-01-11 Novozymes Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic Fusarium expression system and promoters and terminators for use therein
WO1996002563A1 (en) 1994-07-13 1996-02-01 Cornell Research Foundation, Inc. Epstein-barr virus nuclear antigen 1 protein and its expression and recovery
US5602004A (en) * 1994-07-20 1997-02-11 Novo Nordisk Biotech, Inc. Thermophilic fungal expression system
AU4940096A (en) 1995-03-20 1996-10-08 Novo Nordisk A/S Host cell expressing reduced levels of a metalloprotease and methods using the host cell in protein production
US6046021A (en) * 1995-04-12 2000-04-04 Biolog, Inc. Comparative phenotype analysis of two or more microorganisms using a plurality of substrates within a multiwell testing device
AU723619B2 (en) * 1995-04-24 2000-08-31 Chromaxome Corporation Methods for generating and screening novel metabolic pathways
US6030779A (en) * 1995-07-18 2000-02-29 Diversa Corporation Screening for novel bioactivities
US5958672A (en) * 1995-07-18 1999-09-28 Diversa Corporation Protein activity screening of clones having DNA from uncultivated microorganisms
US6057103A (en) * 1995-07-18 2000-05-02 Diversa Corporation Screening for novel bioactivities
FI103133B (fi) 1995-09-01 1999-04-30 Valtion Teknillinen Menetelmä glukoosirepression modifioimiseksi
EP0796328A2 (en) 1995-10-13 1997-09-24 Gist-Brocades B.V. Protein detection
US5939250A (en) * 1995-12-07 1999-08-17 Diversa Corporation Production of enzymes having desired activities by mutagenesis
US5830696A (en) * 1996-12-05 1998-11-03 Diversa Corporation Directed evolution of thermophilic enzymes
US5776730A (en) * 1995-12-15 1998-07-07 University Of Hawaii Neurospora hosts for the production of recombinant proteins, and methods for producing same
AU1750697A (en) * 1996-01-19 1997-08-11 Novo Nordisk Biotech, Inc. Morphological mutants of filamentous fungi
DE69704845T2 (de) 1996-01-26 2001-12-20 Novozymes As Herstellung von toilettenpapier
US5753477A (en) * 1996-03-19 1998-05-19 University Technology Corporation Magneto-biolistic methods
US5783431A (en) * 1996-04-24 1998-07-21 Chromaxome Corporation Methods for generating and screening novel metabolic pathways
US7883872B2 (en) * 1996-10-10 2011-02-08 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
US5811381A (en) * 1996-10-10 1998-09-22 Mark A. Emalfarb Cellulase compositions and methods of use
US5879921A (en) * 1996-11-07 1999-03-09 Novo Nordisk A/S Recombinant expression of a glucose oxidase from a cladosporium strain
JP4275741B2 (ja) * 1996-12-13 2009-06-10 ノバルティス バクシンズ アンド ダイアグノスティックス， インコーポレーテッド 血小板由来増殖因子タンパク質の分析および分離
US5989814A (en) * 1997-04-01 1999-11-23 Reagents Of The University Of California Screening methods in eucaryotic cells
US6573068B1 (en) * 1997-11-13 2003-06-03 Genset, S. A. Claudin-50 protein
ES2143408B1 (es) 1998-04-02 2000-12-01 Urquima Sa Promotor y construcciones para expresion de proteinas recombinantes en hongos filamentosos.
US5914245A (en) 1998-04-20 1999-06-22 Kairos Scientific Inc. Solid phase enzyme kinetics screening in microcolonies
US6413776B1 (en) 1998-06-12 2002-07-02 Galapagos Geonomics N.V. High throughput screening of gene function using adenoviral libraries for functional genomics applications
EP1088229A4 (en) 1998-06-25 2002-05-08 Caliper Techn Corp HIGH THROUGHPUT METHODS, SYSTEMS AND APPARATUS FOR CELL SCREENING TEST
ATE547532T1 (de) 1998-06-29 2012-03-15 Bristol Myers Squibb Co Verfahren zur erzeugung von hochgradig diversen bibliotheken
KR100618495B1 (ko) * 1998-10-06 2006-08-31 마크 아론 에말파브 섬유상의 진균성 숙주 영역에서의 형질전환 시스템:크리소스포륨속에서
US6518042B1 (en) * 1999-02-24 2003-02-11 Novozymes A/S Process for making DNA libraries in filamentous fungal cells using a novel cloned gene involved in the mismatch repair system of filamentous fungal cells
JP4620253B2 (ja) 1999-03-22 2011-01-26 ノボザイムス，インコーポレイティド 菌類細胞中で遺伝子を発現させるためのプロモーター
US6121034A (en) * 1999-05-13 2000-09-19 Her Majesty The Queen In Right Of Canada, As Represented By The Minister Of Agriculture And Agri-Food Coniothyrium minitans xylanase gene Cxy1
WO2001025468A1 (en) 1999-10-06 2001-04-12 Mark Aaron Emalfarb High-throughput screening of expressed dna libraries in filamentous fungi
JP5138855B2 (ja) 2000-04-13 2013-02-06 ダイアディック・インターナショナル・（ユーエスエイ）・インコーポレーテッド 糸状菌において発現されるｄｎａライブラリーのハイスループットスクリーニング
CZ303980B6 (cs) * 2000-04-13 2013-07-31 Dyadic International (Usa), Inc. Expresi regulující sekvence nukleové kyseliny a produkty exprese v oblasti vláknitých plísní
KR20020026456A (ko) * 2000-04-13 2002-04-10 에말파브 마크 아론 사상균에서 발현된 ｄｎａ 라이브러리의 고산출량 스크리닝
WO2002064741A2 (en) * 2001-02-13 2002-08-22 Diadexus, Inc. Compositions and methods relating to breast specific genes and proteins
AU2003279841A1 (en) 2002-10-07 2004-05-04 Zymogenetics, Inc. Uses of human zven antagonists
ATE438711T1 (de) * 2002-12-20 2009-08-15 Novozymes As Polypeptide mit cellobiohydrolase ii-aktivität und dafür kodierende polynucleotide
ES2469874T3 (es) * 2004-01-30 2014-06-20 Novozymes Inc Polip�ptidos con actividad de mejora celulol�tica y polinucle�tidos que los codifican
US7271244B2 (en) * 2004-02-06 2007-09-18 Novozymes, Inc. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
US7332319B2 (en) * 2004-05-27 2008-02-19 Genencor International, Inc. Heterologous alpha amylase expression in Aspergillus
US20060105361A1 (en) * 2004-08-24 2006-05-18 Rothstein Rodney J Donor yeast strain for transfer of genetic material
MX2007008050A (es) * 2005-01-06 2007-08-21 Novozymes Inc Polipeptidos que tienen actividad de celobiohidrolasa y polinucleotidos que codifican para los mismos.
BRPI0616721A2 (pt) * 2005-09-30 2011-06-28 Novozymes Inc métodos para degradar ou converter um material celulósico e para produzir uma substáncia, e, composição detergente
US7923236B2 (en) * 2007-08-02 2011-04-12 Dyadic International (Usa), Inc. Fungal enzymes

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US8268585B2 (en) 2012-09-18
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US8871493B2 (en) 2014-10-28
AU771539C (en) 2005-01-13
ATE286136T1 (de) 2005-01-15
WO2000020555A3 (en) 2000-10-05
US20080194005A1 (en) 2008-08-14
CA2345356A1 (en) 2000-04-13
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EP1117808A2 (en) 2001-07-25
US7399627B2 (en) 2008-07-15
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