CN102154340B - 一种获得高效表达脂肪酶菌株的方法 - Google Patents
一种获得高效表达脂肪酶菌株的方法 Download PDFInfo
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Images
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/43—Enzymes; Proenzymes; Derivatives thereof
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- A61K38/465—Hydrolases (3) acting on ester bonds (3.1), e.g. lipases, ribonucleases
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/06—Antihyperlipidemics
Abstract
Description
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Application Number | Priority Date | Filing Date | Title |
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CN2011100005340A CN102154340B (zh) | 2011-01-04 | 2011-01-04 | 一种获得高效表达脂肪酶菌株的方法 |
PCT/CN2011/076460 WO2012092760A1 (zh) | 2011-01-04 | 2011-06-28 | 一种获得高效表达脂肪酶菌株的方法以及获得的脂肪酶的应用 |
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CN2011100005340A CN102154340B (zh) | 2011-01-04 | 2011-01-04 | 一种获得高效表达脂肪酶菌株的方法 |
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CN102154340A CN102154340A (zh) | 2011-08-17 |
CN102154340B true CN102154340B (zh) | 2013-07-10 |
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WO (1) | WO2012092760A1 (zh) |
Families Citing this family (4)
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CN102864164B (zh) * | 2011-07-04 | 2014-04-09 | 深圳市绿微康生物工程有限公司 | 扩展青霉菌脂肪酶在制备生物柴油中的应用 |
CN103757047A (zh) * | 2014-01-06 | 2014-04-30 | 上海交通大学 | 在扩展青霉细胞内表达外源蛋白的表达设备及基因工程菌 |
CN103757012A (zh) * | 2014-01-06 | 2014-04-30 | 上海交通大学 | 扩展青霉分泌表达外源蛋白的表达设备及其基因工程菌 |
CN111349648A (zh) * | 2020-02-28 | 2020-06-30 | 浙江工业大学 | 一种农杆菌介导外源基因导入蛹虫草细胞的方法 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7122330B2 (en) * | 2000-04-13 | 2006-10-17 | Mark Aaron Emalfarb | High-throughput screening of expressed DNA libraries in filamentous fungi |
US7399627B2 (en) * | 1998-10-06 | 2008-07-15 | Dyadic International (Usa), Inc. | Transformation system in the field of filamentous fungal hosts |
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CN1168821C (zh) * | 2001-10-11 | 2004-09-29 | 李宝健 | 农杆菌介导外源基因转化灵芝的方法 |
CN102154238A (zh) * | 2011-01-04 | 2011-08-17 | 深圳市绿微康生物工程有限公司 | 一种有效降低人体胆固醇、血脂水平的脂肪酶及其应用 |
CN102154358B (zh) * | 2011-01-04 | 2013-07-10 | 深圳市绿微康生物工程有限公司 | 一种高效表达的产脂肪酶基因质粒的构建方法 |
CN102154237A (zh) * | 2011-01-04 | 2011-08-17 | 深圳市绿微康生物工程有限公司 | 一种有效降低动物体内胆固醇、血脂水平的脂肪酶及其应用 |
-
2011
- 2011-01-04 CN CN2011100005340A patent/CN102154340B/zh active Active
- 2011-06-28 WO PCT/CN2011/076460 patent/WO2012092760A1/zh active Application Filing
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7399627B2 (en) * | 1998-10-06 | 2008-07-15 | Dyadic International (Usa), Inc. | Transformation system in the field of filamentous fungal hosts |
US7122330B2 (en) * | 2000-04-13 | 2006-10-17 | Mark Aaron Emalfarb | High-throughput screening of expressed DNA libraries in filamentous fungi |
Non-Patent Citations (2)
Title |
---|
邢伟.《青霉遗传转化系统的研究进展》.《广西轻工业》.2010,(第3期),全文. * |
陈丹.《扩展青霉碱性脂肪酶的高效表达与活性研究》.《中国优秀硕士论文全文数据库》.2007,(第3期),全文. * |
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CN102154340A (zh) | 2011-08-17 |
WO2012092760A1 (zh) | 2012-07-12 |
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