ATE286136T1 - Transformationsystem in filamentösen fungiziden chrysosporium-wirtszellen - Google Patents

Transformationsystem in filamentösen fungiziden chrysosporium-wirtszellen

Info

Publication number
ATE286136T1
ATE286136T1 AT99949459T AT99949459T ATE286136T1 AT E286136 T1 ATE286136 T1 AT E286136T1 AT 99949459 T AT99949459 T AT 99949459T AT 99949459 T AT99949459 T AT 99949459T AT E286136 T1 ATE286136 T1 AT E286136T1
Authority
AT
Austria
Prior art keywords
chrysosporium
filamentous
transformation system
sequences
fungicide
Prior art date
Application number
AT99949459T
Other languages
English (en)
Inventor
Mark Aaron Emalfarb
Richard Paul Burlingame
Philip Terry Olson
Arkady Panteleimonovi Sinitsyn
Martine Parriche
Jean Christophe Bousson
Christine Marie Pynnonen
Peter Jan Punt
Zeijl Cornelia Maria Johan Van
Original Assignee
Mark Aaron Emalfarb
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mark Aaron Emalfarb filed Critical Mark Aaron Emalfarb
Application granted granted Critical
Publication of ATE286136T1 publication Critical patent/ATE286136T1/de

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2434Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2462Lysozyme (3.2.1.17)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477Hemicellulases not provided in a preceding group
    • C12N9/248Xylanases
    • C12N9/2482Endo-1,4-beta-xylanase (3.2.1.8)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008Endo-1,4-beta-xylanase (3.2.1.8)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091Cellulose 1,4-beta-cellobiosidase (3.2.1.91)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Radio Relay Systems (AREA)
  • Peptides Or Proteins (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
AT99949459T 1998-10-06 1999-10-06 Transformationsystem in filamentösen fungiziden chrysosporium-wirtszellen ATE286136T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EPPCT/EP98/06496 1998-10-06
PCT/NL1999/000618 WO2000020555A2 (en) 1998-10-06 1999-10-06 Transformation system in the field of filamentous fungal hosts: in chrysosporium

Publications (1)

Publication Number Publication Date
ATE286136T1 true ATE286136T1 (de) 2005-01-15

Family

ID=8167095

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99949459T ATE286136T1 (de) 1998-10-06 1999-10-06 Transformationsystem in filamentösen fungiziden chrysosporium-wirtszellen

Country Status (13)

Country Link
US (4) US6573086B1 (de)
EP (1) EP1117808B1 (de)
KR (1) KR100618495B1 (de)
CN (1) CN1230546C (de)
AT (1) ATE286136T1 (de)
AU (1) AU771539C (de)
BR (1) BRPI9914278B1 (de)
CA (1) CA2345356C (de)
DE (1) DE69922978T2 (de)
DK (1) DK1117808T3 (de)
EA (1) EA005682B1 (de)
ES (1) ES2237159T3 (de)
WO (1) WO2000020555A2 (de)

Families Citing this family (102)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7883872B2 (en) 1996-10-10 2011-02-08 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
ATE286136T1 (de) 1998-10-06 2005-01-15 Mark Aaron Emalfarb Transformationsystem in filamentösen fungiziden chrysosporium-wirtszellen
ES2345075T3 (es) * 1998-10-23 2010-09-14 Meiji Seika Kaisha Ltd. Endoglucanasas y preparaciones de celulasa que las contienen.
KR100312070B1 (ko) * 1999-03-15 2001-11-03 박호군 고속 선별법을 포함하는 개선된 형질을 갖는 효소 변이체의 제조방법
US7122330B2 (en) 2000-04-13 2006-10-17 Mark Aaron Emalfarb High-throughput screening of expressed DNA libraries in filamentous fungi
AU782105B2 (en) * 2000-04-13 2005-07-07 Dyadic International (Usa), Inc. Novel expression-regulating sequences and expression products in the field of filamentous fungi
DE60125534T2 (de) 2000-05-22 2007-04-19 Meiji Seika Kaisha Ltd. Endoglukanase nce5
DK2279755T3 (da) 2001-10-10 2014-05-26 Ratiopharm Gmbh Remodellering og glycokonjugering af fibroblastvækstfaktor (FGF)
WO2004099231A2 (en) 2003-04-09 2004-11-18 Neose Technologies, Inc. Glycopegylation methods and proteins/peptides produced by the methods
FI118339B (fi) * 2004-09-21 2007-10-15 Ab Enzymes Oy Uusi lakkaasientsyymi ja sen käyttö
EP2292747B1 (de) * 2004-12-30 2017-01-25 Danisco US Inc. Cbh2-cellulasevarianten aus hypocrea jecorina
MX2008008225A (es) * 2005-12-22 2008-10-01 Ab Enzymes Oy Nuevas enzimas.
US7256032B2 (en) 2005-12-22 2007-08-14 Ab Enzymes Oy Enzymes
FI119325B (fi) * 2005-12-22 2008-10-15 Ab Enzymes Oy Uusia endoglukanaasi polypeptidejä ja niiden tuotto ja käyttö
DK1999257T3 (da) 2006-03-20 2011-10-31 Novozymes Inc Polypeptider, der har endo-glucosidaseaktivitet og polynukleotider, der koder for samme
DK2004819T3 (en) * 2006-04-05 2015-10-05 Danisco Us Inc FILAMENTOUS FUNGI WITH REDUCED CONTENT OF UDP-GALACTOFURANOSE
WO2008008793A2 (en) * 2006-07-10 2008-01-17 Dyadic International Inc. Methods and compositions for degradation of lignocellulosic material
US20080026376A1 (en) 2006-07-11 2008-01-31 Huaming Wang KEX2 cleavage regions of recombinant fusion proteins
EP2102366A4 (de) 2006-12-10 2010-01-27 Dyadic International Inc Expression und hochdurchsatz-screening komplexer exprimierter dna-bibliotheken bei fadenpilzen
US9862956B2 (en) 2006-12-10 2018-01-09 Danisco Us Inc. Expression and high-throughput screening of complex expressed DNA libraries in filamentous fungi
DK2069491T3 (en) 2007-05-10 2018-04-09 Novozymes Inc COMPOSITIONS AND PROCEDURES FOR IMPROVING THE DECOMPOSITION OR CONVERSION OF CELLULOSE SUBSTANCES
US7923236B2 (en) 2007-08-02 2011-04-12 Dyadic International (Usa), Inc. Fungal enzymes
EP2197893B1 (de) * 2007-09-07 2013-07-24 Dyadic International, Inc. Neue pilzenzyme
WO2010107303A2 (en) 2009-03-16 2010-09-23 Dyadic Nederland B.V. New fungal production system
WO2010120557A1 (en) 2009-03-31 2010-10-21 Codexis, Inc. Improved endoglucanases
WO2010129287A2 (en) 2009-04-27 2010-11-11 The Board Of Trustees Of The University Of Illinois Hemicellulose-degrading enzymes
FI121712B (fi) * 2009-04-30 2011-03-15 Ab Enzymes Oy Uusi sieniperäinen proteaasi ja sen käyttö
FI121711B (fi) 2009-04-30 2011-03-15 Ab Enzymes Oy Sieniperäinen seriiniproteaasi ja sen käyttö
CA2765715A1 (en) 2009-06-16 2010-12-23 Codexis, Inc. .beta.-glucosidase variants
FI121851B (fi) 2009-07-08 2011-05-13 Ab Enzymes Oy Sieniperäinen proteaasi ja sen käyttö
JP5932648B2 (ja) 2009-09-23 2016-06-15 ダニスコ・ユーエス・インク 新規なグリコシルヒドロラーゼ酵素及びその用途
DK2483415T3 (en) 2009-09-30 2015-02-09 Codexis Inc RECOMBINANT C1 B-glucosidase FOR MANUFACTURE OF sugars from cellulosic BIOMASS
US8652813B2 (en) 2009-11-25 2014-02-18 Codexis, Inc. Recombinant Thermoascus aurantiacus β-glucosidase variants
MX2012006016A (es) 2009-11-25 2012-06-25 Codexis Inc Variantes de beta-glucosidasa recombinante para produccion de azucares solubles de biomasa celulosica.
DE102009055015B4 (de) * 2009-12-18 2013-12-24 Technische Universität Dresden Hefe-Sekretionssystem auf Basis einer Signalsequenz aus der Hydrophobin 1-Gensequenz von Trichoderma reesei
PH12012500999A1 (en) 2009-12-23 2013-02-11 Danisco Us Inc Methods for improving the efficiency of simultaneous saccharification and fermentation reactions
FI122937B (fi) 2009-12-30 2012-09-14 Roal Oy Menetelmä selluloosamateriaalin käsittelemiseksi sekä tässä käyttökelpoiset CBH II/Cel6A entsyymit
CN103080306B (zh) 2010-08-20 2015-02-18 科德克希思公司 糖苷水解酶61家族蛋白在纤维素加工中的用途
WO2012024662A2 (en) * 2010-08-20 2012-02-23 Codexis, Inc. Expression constructs comprising fungal promoters
KR20130132416A (ko) * 2010-10-20 2013-12-04 다니스코 유에스 인크. 열안정성 트리코데르마 셀룰라아제
FI123942B (fi) 2010-10-29 2013-12-31 Ab Enzymes Oy Sieniperäisen seriiniproteaasin variantteja
ES2570382T3 (es) 2010-11-02 2016-05-18 Codexis Inc Composiciones y métodos para la producción de azúcares fermentables
US9068235B2 (en) 2010-11-02 2015-06-30 Codexis, Inc. Fungal strains
WO2012068236A2 (en) * 2010-11-16 2012-05-24 Dyadic International (Usa) Inc. Novel fungal oxidoreductases
CN102154358B (zh) * 2011-01-04 2013-07-10 深圳市绿微康生物工程有限公司 一种高效表达的产脂肪酶基因质粒的构建方法
CN102154340B (zh) * 2011-01-04 2013-07-10 深圳市绿微康生物工程有限公司 一种获得高效表达脂肪酶菌株的方法
CN102154238A (zh) * 2011-01-04 2011-08-17 深圳市绿微康生物工程有限公司 一种有效降低人体胆固醇、血脂水平的脂肪酶及其应用
CN102154237A (zh) * 2011-01-04 2011-08-17 深圳市绿微康生物工程有限公司 一种有效降低动物体内胆固醇、血脂水平的脂肪酶及其应用
US20140073017A1 (en) 2011-03-17 2014-03-13 Danisco Us Inc. Cellulase compositions and methods of using the same for improved conversion of lignocellulosic biomass into fermentable sugars
JP6290626B2 (ja) 2011-03-17 2018-03-14 ダニスコ・ユーエス・インク 糖化プロセスにおける粘度を低下させる方法
FI123425B (fi) 2011-03-31 2013-04-30 Ab Enzymes Oy Proteaasientsyymi ja sen käytöt
CN103014036A (zh) * 2011-07-01 2013-04-03 深圳市绿微康生物工程有限公司 改型扩展青霉脂肪酶基因及其构建和表达方法
EP2748317B1 (de) 2011-08-22 2017-04-19 Codexis, Inc. Gh61-glykosidhydrolase-proteinvarianten und kofaktoren zur verstärkung der gh61-aktivität
US20130084608A1 (en) 2011-09-30 2013-04-04 Codexis, Inc. Fungal proteases
WO2013067026A1 (en) 2011-10-31 2013-05-10 Bp Corporation North America Inc. Use of plant promoters in filamentous fungi
US20130109055A1 (en) 2011-10-31 2013-05-02 Bp Corporation North America Inc. Use of mammalian promoters in filamentous fungi
US20140234897A1 (en) * 2011-11-04 2014-08-21 Novozymes Inc. Polypeptides Having Cellobiohydrolase Activity and Polynucleotides Encoding Same
EP2794871A4 (de) 2011-12-20 2015-12-30 Codexis Inc Varianten von endoglucanase-1b (eg1b)
US8980578B2 (en) 2012-06-11 2015-03-17 Codexis, Inc. Fungal beta-xylosidase variants
US20150376590A1 (en) 2012-11-02 2015-12-31 Bp Corporation North America Inc. Thermotolerant Beta-Glucosidase Variants
WO2014145768A2 (en) 2013-03-15 2014-09-18 Bp Corporation North America Inc. Use of non-fungal 5' utrs in filamentous fungi
ES2521940B1 (es) 2013-05-10 2015-09-09 Abengoa Bioenergía Nuevas Tecnologías, S.A. Variantes de beta-glucosidasa con actividad de transglicosilación reducida
ES2727390T3 (es) 2013-05-20 2019-10-15 Abengoa Bioenergia Nuevas Tecnologias Sa Expresión de enzimas beta-xilosidasas recombinantes
ES2527368B1 (es) 2013-07-22 2015-10-27 Abengoa Bioenergía Nuevas Tecnologías, S.A. Célula hospedadora de Myceliophthora thermophila que expresa una enzima alfa-xilosidasa heteróloga y su uso en un procedimiento de degradación de biomasa
EP3074513A1 (de) 2013-11-26 2016-10-05 Novozymes A/S Enzymzusammensetzungen und verwendungen davon
ES2542621B1 (es) 2014-02-07 2016-08-16 Abengoa Bioenergía Nuevas Tecnologías, S.A. Polipéptidos con actividad polisacárido monooxigenasa y su uso para la producción de azúcares fermentables
EP3152315B1 (de) 2014-06-06 2018-08-15 Novozymes A/S Enzymzusammensetzungen und ihre verwendungen
CN108699578A (zh) 2015-03-11 2018-10-23 杰能科国际有限公司 裂解性多糖单加氧酶的酶活性
HUE053863T2 (hu) 2015-05-07 2021-07-28 Codexis Inc Penicillin-G acilázok
US20190002846A1 (en) 2015-06-24 2019-01-03 Genencor International B.V. Polypeptides having demethylating activity
BR112018001855A2 (pt) 2015-07-29 2018-09-25 Abengoa Bioenergia Nuevas Tecnologias Sa expressão de enzimas beta-xilosidase recombinantes
JP2019503168A (ja) 2015-12-02 2019-02-07 ビーエーエスエフ ソシエタス・ヨーロピアBasf Se Clr2活性が低減した糸状菌においてタンパク質を生産する方法
CN108291191A (zh) 2015-12-02 2018-07-17 巴斯夫欧洲公司 在具有降低的clr1活性的丝状真菌中生产蛋白质的方法
US11293029B2 (en) 2015-12-07 2022-04-05 Zymergen Inc. Promoters from Corynebacterium glutamicum
US11208649B2 (en) 2015-12-07 2021-12-28 Zymergen Inc. HTP genomic engineering platform
US9988624B2 (en) 2015-12-07 2018-06-05 Zymergen Inc. Microbial strain improvement by a HTP genomic engineering platform
DK3419992T3 (da) 2016-02-22 2021-02-15 Danisco Us Inc Svampesystem til proteinproduktion med højt udbytte
DK3452587T3 (da) 2016-05-05 2021-10-11 Codexis Inc Penicillin-g-acylaser
ES2647322B1 (es) * 2016-05-20 2018-10-05 Abengoa Bioenergía Nuevas Tecnologías, S.A. Célula hospedadora Myceliophthora thermophila con actividad celulolítica mejorada y composiciones enzimáticas obtenidas por la misma
CN109642226A (zh) 2016-06-30 2019-04-16 丹尼斯科美国公司 天冬氨酸蛋白酶
WO2018005655A2 (en) 2016-06-30 2018-01-04 Zymergen Inc. Methods for generating a bacterial hemoglobin library and uses thereof
EP3478845A4 (de) 2016-06-30 2019-07-31 Zymergen, Inc. Verfahren zur erzeugung einer glucose-permease-bibliothek und verwendungen davon
WO2018129130A1 (en) 2017-01-05 2018-07-12 Codexis, Inc. Penicillin-g acylases
EP3378936B1 (de) * 2017-03-24 2021-05-12 Clariant International Ltd Cellulase zur verwendung in waschmittelzusammensetzungen
WO2018226900A2 (en) 2017-06-06 2018-12-13 Zymergen Inc. A htp genomic engineering platform for improving fungal strains
CN111032682B (zh) 2017-06-14 2024-04-12 科德克希思公司 用于工业生物催化的工程化转氨酶多肽
EP3645713A4 (de) 2017-06-27 2021-06-30 Codexis, Inc. Penicillin-g-acylasen
JP2020533966A (ja) 2017-08-21 2020-11-26 ダイアディク インターナショナル インコーポレイテッド Myceliophthora thermophila中でのフルワクチンの産生
JP2021502068A (ja) 2017-11-07 2021-01-28 コデクシス, インコーポレイテッド トランスグルタミナーゼバリアント
CN109988715B (zh) * 2017-12-29 2022-03-01 青岛蔚蓝生物集团有限公司 一种高产木聚糖酶的突变菌株及其应用
CN110093332B (zh) * 2018-01-30 2021-12-28 青岛蔚蓝生物集团有限公司 一种纤维素酶突变体及其高产菌株
KR20210018219A (ko) 2018-06-06 2021-02-17 지머젠 인코포레이티드 발효 및 생산 중 곰팡이 형태를 제어하기 위한 신호 전달에 포함된 유전자의 조작
US11274286B2 (en) 2018-07-09 2022-03-15 Codexis, Inc. Engineered deoxyribose-phosphate aldolases
CN108913713A (zh) * 2018-07-24 2018-11-30 中国农业科学院饲料研究所 提高里氏木霉转化效率的重组表达载体及应用
EP3636735B1 (de) 2018-10-12 2024-03-27 AB Enzymes Oy Variante einer protease und die verwendung davon
WO2021207200A2 (en) 2020-04-10 2021-10-14 Codexis, Inc. Engineered transaminase polypeptides
WO2021220209A1 (en) 2020-04-30 2021-11-04 Dyadic International (Usa), Inc. Nicotinamide riboside production in filamentous fungi
US11479779B2 (en) * 2020-07-31 2022-10-25 Zymergen Inc. Systems and methods for high-throughput automated strain generation for non-sporulating fungi
CN113403209B (zh) * 2021-07-30 2022-08-26 西南大学 天冬氨酸蛋白酶基因在改良球孢白僵菌菌株中的应用
CN121127575A (zh) 2022-12-19 2025-12-12 巴斯夫欧洲公司 用于真菌蛋白生产的新型纤维素酶启动子
WO2024156593A1 (en) 2023-01-27 2024-08-02 Basf Se New genomic integration site for fungal protein and fine chemicals production
WO2025262103A1 (en) 2024-06-19 2025-12-26 Basf Se Superior promoters for fungal protein production

Family Cites Families (111)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2974001A (en) * 1957-07-20 1961-03-07 Kalle Ag Process and compositions for enzymatic desizing and bleaching of textiles
GB1368599A (en) 1970-09-29 1974-10-02 Unilever Ltd Softening compositions
JPS5028515B2 (de) * 1971-09-30 1975-09-16
US3966543A (en) * 1972-10-30 1976-06-29 Baxter Laboratories, Inc. Enzyme-treated paper
US4081328A (en) * 1975-10-23 1978-03-28 Stanford Research Institute Production of cellulase by a thermophilic thielavia terrestris
DK187280A (da) * 1980-04-30 1981-10-31 Novo Industri As Ruhedsreducerende middel til et fuldvaskemiddel fuldvaskemiddel og fuldvaskemetode
GB2094826B (en) 1981-03-05 1985-06-12 Kao Corp Cellulase enzyme detergent composition
JPS591598A (ja) * 1982-06-25 1984-01-06 花王株式会社 洗浄剤組成物
US4486533A (en) * 1982-09-02 1984-12-04 St. Louis University Filamentous fungi functional replicating extrachromosomal element
NL8300698A (nl) * 1983-02-24 1984-09-17 Univ Leiden Werkwijze voor het inbouwen van vreemd dna in het genoom van tweezaadlobbige planten; agrobacterium tumefaciens bacterien en werkwijze voor het produceren daarvan; planten en plantecellen met gewijzigde genetische eigenschappen; werkwijze voor het bereiden van chemische en/of farmaceutische produkten.
GB8306645D0 (en) * 1983-03-10 1983-04-13 Unilever Plc Detergent compositions
US4462307A (en) * 1983-05-23 1984-07-31 Pet Incorporated Humpback oven-broiler
GB8421800D0 (en) * 1984-08-29 1984-10-03 Unilever Plc Detergent compositions
GB8422238D0 (en) 1984-09-03 1984-10-10 Neuberger M S Chimeric proteins
US4816405A (en) * 1984-10-24 1989-03-28 The Regents Of The University Of California Vectors for transformation by ascomycetes
US4885249A (en) * 1984-12-05 1989-12-05 Allelix, Inc. Aspergillus niger transformation system
US4610800A (en) * 1985-01-25 1986-09-09 Genex Corporation Method for unclogging drainage pipes
US5198345A (en) * 1985-04-15 1993-03-30 Gist-Brocades N.V. Vectors in use in filamentous fungi
US5364770A (en) * 1985-08-29 1994-11-15 Genencor International Inc. Heterologous polypeptides expressed in aspergillus
EP0215594B2 (de) 1985-08-29 2003-10-15 Genencor International, Inc. Expression von heterologen Polypeptiden in filamentösen Pilzen, Verfahren zu deren Herstellung und Vektoren zu deren Herstellung
DK163591C (da) 1985-10-08 1992-08-24 Novo Nordisk As Fremgangsmaade til behandling af et tekstilstof med en cellulase
US4935349A (en) * 1986-01-17 1990-06-19 Zymogenetics, Inc. Expression of higher eucaryotic genes in aspergillus
US5536661A (en) * 1987-03-10 1996-07-16 Novo Nordisk A/S Process for the production of protein products in aspergillus
US5766912A (en) * 1986-03-17 1998-06-16 Novo Nordisk A/S Humicola lipase produced in aspergillus
GB8607679D0 (en) 1986-03-27 1986-04-30 Winter G P Recombinant dna product
US5763192A (en) * 1986-11-20 1998-06-09 Ixsys, Incorporated Process for obtaining DNA, RNA, peptides, polypeptides, or protein, by recombinant DNA technique
US5252726A (en) * 1987-09-04 1993-10-12 Novo Nordisk A/S Promoters for use in aspergillus
US4832864A (en) * 1987-09-15 1989-05-23 Ecolab Inc. Compositions and methods that introduce variations in color density into cellulosic fabrics, particularly indigo dyed denim
US5223409A (en) * 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
US5122159A (en) * 1988-09-15 1992-06-16 Ecolab Inc. Cellulase compositions and methods that introduce variations in color density into cellulosic fabrics, particularly indigo dyed denim
US5006126A (en) * 1988-09-15 1991-04-09 Ecolab Inc. Cellulase compositions and methods that introduce variations in color density into cellulosic fabrics, particularly indigo dyed denim
IL162181A (en) 1988-12-28 2006-04-10 Pdl Biopharma Inc A method of producing humanized immunoglubulin, and polynucleotides encoding the same
US5571697A (en) * 1989-05-05 1996-11-05 Baylor College Of Medicine Texas Medical Center Expression of processed recombinant lactoferrin and lactoferrin polypeptide fragments from a fusion product in Aspergillus
AU6355190A (en) 1989-06-13 1991-01-17 Smithkline Beecham Corporation Inhibition of interleukin-1 and tumor necrosis factor production by monocytes and/or macrophages
ES2150188T3 (es) 1989-07-07 2000-11-16 Unilever Nv Procedimiento para la preparacion de una proteina mediante un hongo transformado por integracion multicopia de un vector de expresion.
US5120463A (en) * 1989-10-19 1992-06-09 Genencor International, Inc. Degradation resistant detergent compositions based on cellulase enzymes
GB8928874D0 (en) 1989-12-21 1990-02-28 Celltech Ltd Humanised antibodies
JPH05505308A (ja) * 1990-03-13 1993-08-12 ハワイ・バイオテクノロジー・グループ・インコーポレイテツド アオパンカビ発現システム
DK115890D0 (da) * 1990-05-09 1990-05-09 Novo Nordisk As Enzym
GB9015198D0 (en) * 1990-07-10 1990-08-29 Brien Caroline J O Binding substance
KR930701625A (ko) * 1990-07-13 1993-06-12 리처드 에프. 셀든 라이브러리 스크리닝 방법
EP0542810A1 (de) * 1990-08-02 1993-05-26 B.R. Centre Limited Verfahren zur herstellung von proteinen mit einer gewünschten funktion
JP3133774B2 (ja) * 1990-10-04 2001-02-13 日本食品化工株式会社 新規な宿主−ベクター系
US5290474A (en) * 1990-10-05 1994-03-01 Genencor International, Inc. Detergent composition for treating cotton-containing fabrics containing a surfactant and a cellulase composition containing endolucanase III from trichoderma ssp
US5780279A (en) * 1990-12-03 1998-07-14 Genentech, Inc. Method of selection of proteolytic cleavage sites by directed evolution and phagemid display
JPH06503960A (ja) * 1990-12-10 1994-05-12 ジェネンコア インターナショナル インコーポレーテッド TRICHODERMA REESEIのβ−グルコシダーゼ遺伝子のクローニングおよび増幅によるセルロースの改良糖化
GB9102635D0 (en) 1991-02-07 1991-03-27 British Bio Technology Compounds
US5858657A (en) * 1992-05-15 1999-01-12 Medical Research Council Methods for producing members of specific binding pairs
DE69230142T2 (de) * 1991-05-15 2000-03-09 Cambridge Antibody Technology Ltd. Verfahren zur herstellung von spezifischen bindungspaargliedern
ES2136092T3 (es) * 1991-09-23 1999-11-16 Medical Res Council Procedimientos para la produccion de anticuerpos humanizados.
US5516670A (en) * 1991-09-30 1996-05-14 Kuehnle; Adelheid R. Magnetophoretic particle delivery method and apparatus for the treatment of cells
BE1005432A4 (nl) 1991-10-01 1993-07-20 Dsm Nv Expressiecassette voor heterologe eiwitten.
EP0672149B1 (de) * 1991-12-09 2001-08-08 Unilever Plc Verfahren zur Herstellung/Sekretion eines Proteins durch einen transformiertenSchimmelpilz unter Verwendung von Expressions-/Sekretions- Regulationsregionenaus Aspergillus
CA2062023A1 (en) * 1992-02-10 1993-08-11 Jagroop S. Dahiya Novel fungal strains and use thereof in antibiotic production
JPH08500733A (ja) 1992-08-19 1996-01-30 アルコ グループ リミティド グルコースの存在下で活性的な真菌プロモーター
PT663953E (pt) * 1992-09-04 2002-05-31 Scripps Research Inst Fagemideos que co-expressam um receptor de superficie e uma proteina heterologa de superficie
DE69332030T2 (de) 1992-12-10 2002-10-02 Dsm N.V., Heerlen Herstellung von heterologen proteinen in filamentösen fungi
GB9316883D0 (en) * 1993-08-13 1993-09-29 Univ Leeds Production of heterologous peptices
AU8095594A (en) * 1993-11-02 1995-05-23 Merck & Co., Inc. Dna encoding triol polyketide synthase
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5820866A (en) * 1994-03-04 1998-10-13 National Jewish Center For Immunology And Respiratory Medicine Product and process for T cell regulation
GB2289218A (en) 1994-05-06 1995-11-15 Merck & Co Inc Inhibition of TNFalpha production with agonists of the A2b subtype of the adenosine receptor
AU2733895A (en) * 1994-06-17 1996-01-15 Novo Nordisk A/S A fungus wherein the area gene has been modified and an area gene from aspergillus oryzae
US6060305A (en) * 1994-06-30 2000-05-09 Novo Nordisk Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic Fusarium expression system
CN101659926A (zh) * 1994-06-30 2010-03-03 诺沃奇梅兹有限公司 非毒性、非产毒性、非致病性镰孢属表达系统及所用启动子和终止子
WO1996002563A1 (en) 1994-07-13 1996-02-01 Cornell Research Foundation, Inc. Epstein-barr virus nuclear antigen 1 protein and its expression and recovery
US5602004A (en) * 1994-07-20 1997-02-11 Novo Nordisk Biotech, Inc. Thermophilic fungal expression system
US5861280A (en) 1995-03-20 1999-01-19 Novo Nordisk A/S Host cell expressing reduced levels of a metalloprotease and methods using the host cell in protein production
US6046021A (en) * 1995-04-12 2000-04-04 Biolog, Inc. Comparative phenotype analysis of two or more microorganisms using a plurality of substrates within a multiwell testing device
KR100501838B1 (ko) * 1995-04-24 2005-07-21 큐비스트 파마슈티컬즈 인코포레이티드 새로운 대사 경로를 만들고 이를 스크리닝하는 방법
US5958672A (en) * 1995-07-18 1999-09-28 Diversa Corporation Protein activity screening of clones having DNA from uncultivated microorganisms
US6030779A (en) * 1995-07-18 2000-02-29 Diversa Corporation Screening for novel bioactivities
US6057103A (en) * 1995-07-18 2000-05-02 Diversa Corporation Screening for novel bioactivities
FI103133B (fi) 1995-09-01 1999-04-30 Valtion Teknillinen Menetelmä glukoosirepression modifioimiseksi
EP0796328A2 (de) 1995-10-13 1997-09-24 Gist-Brocades B.V. Protein-detektion
US5830696A (en) * 1996-12-05 1998-11-03 Diversa Corporation Directed evolution of thermophilic enzymes
US5939250A (en) * 1995-12-07 1999-08-17 Diversa Corporation Production of enzymes having desired activities by mutagenesis
US5776730A (en) * 1995-12-15 1998-07-07 University Of Hawaii Neurospora hosts for the production of recombinant proteins, and methods for producing same
WO1997026330A2 (en) * 1996-01-19 1997-07-24 Novo Nordisk Biotech, Inc. Morphological mutants of filamentous fungi
AU1437997A (en) 1996-01-26 1997-08-20 Novo Nordisk A/S Production of sanitary paper
US5753477A (en) * 1996-03-19 1998-05-19 University Technology Corporation Magneto-biolistic methods
US5783431A (en) * 1996-04-24 1998-07-21 Chromaxome Corporation Methods for generating and screening novel metabolic pathways
US7883872B2 (en) * 1996-10-10 2011-02-08 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
US5811381A (en) * 1996-10-10 1998-09-22 Mark A. Emalfarb Cellulase compositions and methods of use
US5879921A (en) * 1996-11-07 1999-03-09 Novo Nordisk A/S Recombinant expression of a glucose oxidase from a cladosporium strain
US6017731A (en) * 1996-12-13 2000-01-25 Chiron Corporation Method for expression of heterologous proteins in yeast
US5989814A (en) * 1997-04-01 1999-11-23 Reagents Of The University Of California Screening methods in eucaryotic cells
US6573068B1 (en) * 1997-11-13 2003-06-03 Genset, S. A. Claudin-50 protein
ES2143408B1 (es) 1998-04-02 2000-12-01 Urquima Sa Promotor y construcciones para expresion de proteinas recombinantes en hongos filamentosos.
US5914245A (en) 1998-04-20 1999-06-22 Kairos Scientific Inc. Solid phase enzyme kinetics screening in microcolonies
US6413776B1 (en) 1998-06-12 2002-07-02 Galapagos Geonomics N.V. High throughput screening of gene function using adenoviral libraries for functional genomics applications
WO1999067639A1 (en) 1998-06-25 1999-12-29 Caliper Technologies Corporation High throughput methods, systems and apparatus for performing cell based screening assays
IL140100A0 (en) 1998-06-29 2002-02-10 Phylos Inc Methods of generating highly diverse libraries
ATE286136T1 (de) * 1998-10-06 2005-01-15 Mark Aaron Emalfarb Transformationsystem in filamentösen fungiziden chrysosporium-wirtszellen
US6518042B1 (en) * 1999-02-24 2003-02-11 Novozymes A/S Process for making DNA libraries in filamentous fungal cells using a novel cloned gene involved in the mismatch repair system of filamentous fungal cells
EP2278016B1 (de) 1999-03-22 2012-09-26 Novozymes Inc. Promotorsequenzen von Fusarium Venenatum und deren Verwendungen
US6121034A (en) * 1999-05-13 2000-09-19 Her Majesty The Queen In Right Of Canada, As Represented By The Minister Of Agriculture And Agri-Food Coniothyrium minitans xylanase gene Cxy1
AU4352700A (en) 1999-10-06 2001-05-10 Mark Aaron Emalfarb High-throughput screening of expressed dna libraries in filamentous fungi
US7122330B2 (en) * 2000-04-13 2006-10-17 Mark Aaron Emalfarb High-throughput screening of expressed DNA libraries in filamentous fungi
CN1380905A (zh) 2000-04-13 2002-11-20 马克·阿龙·埃马尔法尔布 丝状真菌中dna表达文库的高通量筛选
AU782105B2 (en) * 2000-04-13 2005-07-07 Dyadic International (Usa), Inc. Novel expression-regulating sequences and expression products in the field of filamentous fungi
AU2002243972A1 (en) * 2001-02-13 2002-08-28 Diadexus, Inc. Compositions and methods relating to breast specific genes and proteins
US7205110B2 (en) 2002-10-07 2007-04-17 Zymogenetics, Inc. Uses of human Zven antagonists
ES2331067T5 (es) * 2002-12-20 2013-12-26 Novozymes A/S Polipéptidos que tienen actividad celobiohidrolasa II y polinucleótidos para su codificación
ES2468366T3 (es) * 2004-01-30 2014-06-16 Novozymes Inc. Polip�ptidos con actividad de mejora celulol�tica y polinucle�tidos que los codifican
WO2005074656A2 (en) * 2004-02-06 2005-08-18 Novozymes, Inc. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
US7332319B2 (en) * 2004-05-27 2008-02-19 Genencor International, Inc. Heterologous alpha amylase expression in Aspergillus
US20060105361A1 (en) * 2004-08-24 2006-05-18 Rothstein Rodney J Donor yeast strain for transfer of genetic material
CN101137750B (zh) * 2005-01-06 2014-05-21 诺维信股份有限公司 具有纤维二糖水解酶活性的多肽和编码它的多核苷酸
BRPI0616721A2 (pt) * 2005-09-30 2011-06-28 Novozymes Inc métodos para degradar ou converter um material celulósico e para produzir uma substáncia, e, composição detergente
US7923236B2 (en) * 2007-08-02 2011-04-12 Dyadic International (Usa), Inc. Fungal enzymes

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EP1117808A2 (de) 2001-07-25
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US6573086B1 (en) 2003-06-03
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AU6232699A (en) 2000-04-26
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US7399627B2 (en) 2008-07-15
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