ATE286136T1 - Transformationsystem in filamentösen fungiziden chrysosporium-wirtszellen - Google Patents

Transformationsystem in filamentösen fungiziden chrysosporium-wirtszellen

Info

Publication number
ATE286136T1
ATE286136T1 AT99949459T AT99949459T ATE286136T1 AT E286136 T1 ATE286136 T1 AT E286136T1 AT 99949459 T AT99949459 T AT 99949459T AT 99949459 T AT99949459 T AT 99949459T AT E286136 T1 ATE286136 T1 AT E286136T1
Authority
AT
Austria
Prior art keywords
chrysosporium
filamentous
transformation system
sequences
fungicide
Prior art date
Application number
AT99949459T
Other languages
English (en)
Inventor
Mark Aaron Emalfarb
Richard Paul Burlingame
Philip Terry Olson
Arkady Panteleimonovi Sinitsyn
Martine Parriche
Jean Christophe Bousson
Christine Marie Pynnonen
Peter Jan Punt
Zeijl Cornelia Maria Johan Van
Original Assignee
Mark Aaron Emalfarb
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mark Aaron Emalfarb filed Critical Mark Aaron Emalfarb
Application granted granted Critical
Publication of ATE286136T1 publication Critical patent/ATE286136T1/de

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2434Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2462Lysozyme (3.2.1.17)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477Hemicellulases not provided in a preceding group
    • C12N9/248Xylanases
    • C12N9/2482Endo-1,4-beta-xylanase (3.2.1.8)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008Endo-1,4-beta-xylanase (3.2.1.8)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091Cellulose 1,4-beta-cellobiosidase (3.2.1.91)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Radio Relay Systems (AREA)
  • Peptides Or Proteins (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT99949459T 1998-10-06 1999-10-06 Transformationsystem in filamentösen fungiziden chrysosporium-wirtszellen ATE286136T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EPPCT/EP98/06496 1998-10-06
PCT/NL1999/000618 WO2000020555A2 (en) 1998-10-06 1999-10-06 Transformation system in the field of filamentous fungal hosts: in chrysosporium

Publications (1)

Publication Number Publication Date
ATE286136T1 true ATE286136T1 (de) 2005-01-15

Family

ID=8167095

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99949459T ATE286136T1 (de) 1998-10-06 1999-10-06 Transformationsystem in filamentösen fungiziden chrysosporium-wirtszellen

Country Status (13)

Country Link
US (4) US6573086B1 (de)
EP (1) EP1117808B1 (de)
KR (1) KR100618495B1 (de)
CN (1) CN1230546C (de)
AT (1) ATE286136T1 (de)
AU (1) AU771539C (de)
BR (1) BRPI9914278B1 (de)
CA (1) CA2345356C (de)
DE (1) DE69922978T2 (de)
DK (1) DK1117808T3 (de)
EA (1) EA005682B1 (de)
ES (1) ES2237159T3 (de)
WO (1) WO2000020555A2 (de)

Families Citing this family (102)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7883872B2 (en) 1996-10-10 2011-02-08 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
KR100618495B1 (ko) 1998-10-06 2006-08-31 마크 아론 에말파브 섬유상의 진균성 숙주 영역에서의 형질전환 시스템:크리소스포륨속에서
ES2345075T3 (es) * 1998-10-23 2010-09-14 Meiji Seika Kaisha Ltd. Endoglucanasas y preparaciones de celulasa que las contienen.
KR100312070B1 (ko) * 1999-03-15 2001-11-03 박호군 고속 선별법을 포함하는 개선된 형질을 갖는 효소 변이체의 제조방법
US7122330B2 (en) * 2000-04-13 2006-10-17 Mark Aaron Emalfarb High-throughput screening of expressed DNA libraries in filamentous fungi
HUP0300469A2 (hu) 2000-04-13 2003-06-28 Emalfarb, Mark Aaron Új, expressziós szabályzó szekvenciák és expressziós termékek fonalas gombákban
DK1291431T3 (da) 2000-05-22 2007-03-26 Meiji Seika Kaisha Endoglucanase NCE5
SG177002A1 (en) 2001-10-10 2012-01-30 Novo Nordisk As Remodeling and glycoconjugation of peptides
MXPA05010773A (es) 2003-04-09 2005-12-12 Neose Technologies Inc Metodos de glicopegilacion y proteinas/peptidos producidos por los metodos.
FI118339B (fi) * 2004-09-21 2007-10-15 Ab Enzymes Oy Uusi lakkaasientsyymi ja sen käyttö
CA2592550C (en) * 2004-12-30 2015-05-19 Genencor International, Inc. Novel variant hypocrea jecorina cbh2 cellulases
US7256032B2 (en) 2005-12-22 2007-08-14 Ab Enzymes Oy Enzymes
FI119325B (fi) * 2005-12-22 2008-10-15 Ab Enzymes Oy Uusia endoglukanaasi polypeptidejä ja niiden tuotto ja käyttö
BRPI0620318B1 (pt) * 2005-12-22 2019-02-05 Ab Enzymes Oy proteína de fusão endoglicanase, polinucleotídeo isolado, vetor de expressão, célula hospedeira de trichoderma reesei, preparação de enzima, composição detergente, cepa de escherichia coli, e processos para produção de um polipeptídeo endoglicanase, para bioestonagem, para bioacabamento, para tratamento de material têxtil contendo fibra celulósica, para tratamento de polpa ou fibra derivada de madeira, e para aperfeiçoamento de qualidade de alimentação animal
CN103740674A (zh) 2006-03-20 2014-04-23 诺维信股份有限公司 具有内切葡聚糖酶活性的多肽和编码该多肽的多核苷酸
EP2004819B1 (de) * 2006-04-05 2015-07-22 Danisco US Inc. Filamentöse pilze mit verringertem gehalt an udp-galaktofuranose
WO2008008793A2 (en) * 2006-07-10 2008-01-17 Dyadic International Inc. Methods and compositions for degradation of lignocellulosic material
US20080026376A1 (en) * 2006-07-11 2008-01-31 Huaming Wang KEX2 cleavage regions of recombinant fusion proteins
US8680252B2 (en) 2006-12-10 2014-03-25 Dyadic International (Usa), Inc. Expression and high-throughput screening of complex expressed DNA libraries in filamentous fungi
US9862956B2 (en) 2006-12-10 2018-01-09 Danisco Us Inc. Expression and high-throughput screening of complex expressed DNA libraries in filamentous fungi
EP2069491B1 (de) 2007-05-10 2018-01-03 Novozymes Inc. Zusammensetzungen und verfahren zur verstärkung des abbaus oder der umwandlung von cellulosehaltigem material
US8551751B2 (en) 2007-09-07 2013-10-08 Dyadic International, Inc. BX11 enzymes having xylosidase activity
US7923236B2 (en) * 2007-08-02 2011-04-12 Dyadic International (Usa), Inc. Fungal enzymes
ES2678450T3 (es) 2009-03-16 2018-08-10 Danisco Us Inc. Sistema de producción de proteínas en Chrysosporium lucknowense
BRPI1010239A2 (pt) 2009-03-31 2016-10-11 Codexis Inc endoglucanases aprimoradas, derivados e seus usos
US9012186B2 (en) 2009-04-27 2015-04-21 The Board Of Trustees Of The University Of Illinois Hemicellulose-degrading enzymes
FI121711B (fi) 2009-04-30 2011-03-15 Ab Enzymes Oy Sieniperäinen seriiniproteaasi ja sen käyttö
FI121712B (fi) 2009-04-30 2011-03-15 Ab Enzymes Oy Uusi sieniperäinen proteaasi ja sen käyttö
US8772010B2 (en) 2009-06-16 2014-07-08 Codexis, Inc. β-Glucosidase variants
FI121851B (fi) 2009-07-08 2011-05-13 Ab Enzymes Oy Sieniperäinen proteaasi ja sen käyttö
EP3296394B1 (de) 2009-09-23 2020-11-04 Danisco US Inc. Neuartige glycolylhydrolase-enzyme und verwendungen davon
CA2776170C (en) 2009-09-30 2016-01-05 Codexis, Inc. Recombinant c1 .beta.-glucosidase for production of sugars from cellulosic biomass
EP2504431A4 (de) 2009-11-25 2013-10-16 Codexis Inc Rekombinante thermoascus-aurantiacus-beta-glucosidase-varianten zur herstellung von fermentierbaren zuckern aus zellulosischer biomasse
DK2504017T3 (en) 2009-11-25 2014-03-03 Codexis Inc RECOMBINANT BETA-glucosidase OPTIONS FOR PRODUCTION OF soluble sugars FROM cellulosic biomass
DE102009055015B4 (de) * 2009-12-18 2013-12-24 Technische Universität Dresden Hefe-Sekretionssystem auf Basis einer Signalsequenz aus der Hydrophobin 1-Gensequenz von Trichoderma reesei
CN102686736B (zh) 2009-12-23 2017-05-31 丹尼斯科美国公司 提高同步糖化发酵反应效率的方法
FI122937B (fi) 2009-12-30 2012-09-14 Roal Oy Menetelmä selluloosamateriaalin käsittelemiseksi sekä tässä käyttökelpoiset CBH II/Cel6A entsyymit
EP2606131B1 (de) 2010-08-20 2017-03-08 Codexis, Inc. Verwendung von proteinen der glycosidhydrolase-familie 61 bei der verarbeitung von cellulose
WO2012024662A2 (en) * 2010-08-20 2012-02-23 Codexis, Inc. Expression constructs comprising fungal promoters
BR112013009532A2 (pt) * 2010-10-20 2016-07-19 Danisco Us Inc celulase de tricoderma termoestável
FI123942B (fi) 2010-10-29 2013-12-31 Ab Enzymes Oy Sieniperäisen seriiniproteaasin variantteja
DK2635671T3 (en) 2010-11-02 2016-10-24 Codexis Inc Improved fungi strains
ES2570382T3 (es) 2010-11-02 2016-05-18 Codexis Inc Composiciones y métodos para la producción de azúcares fermentables
WO2012068236A2 (en) * 2010-11-16 2012-05-24 Dyadic International (Usa) Inc. Novel fungal oxidoreductases
CN102154237A (zh) * 2011-01-04 2011-08-17 深圳市绿微康生物工程有限公司 一种有效降低动物体内胆固醇、血脂水平的脂肪酶及其应用
CN102154358B (zh) * 2011-01-04 2013-07-10 深圳市绿微康生物工程有限公司 一种高效表达的产脂肪酶基因质粒的构建方法
CN102154238A (zh) * 2011-01-04 2011-08-17 深圳市绿微康生物工程有限公司 一种有效降低人体胆固醇、血脂水平的脂肪酶及其应用
CN102154340B (zh) * 2011-01-04 2013-07-10 深圳市绿微康生物工程有限公司 一种获得高效表达脂肪酶菌株的方法
US20140134677A1 (en) 2011-03-17 2014-05-15 Danisco Us Inc. Method for reducing viscosity in saccharification process
JP6148183B2 (ja) 2011-03-17 2017-06-21 ダニスコ・ユーエス・インク セルラーゼ組成物並びにこれを用いリグノセルロース系バイオマスの発酵性糖質への変換を向上させる方法
FI123425B (fi) 2011-03-31 2013-04-30 Ab Enzymes Oy Proteaasientsyymi ja sen käytöt
CN103014036A (zh) * 2011-07-01 2013-04-03 深圳市绿微康生物工程有限公司 改型扩展青霉脂肪酶基因及其构建和表达方法
DK2748317T3 (en) 2011-08-22 2017-07-17 Codexis Inc GH61 glycoside hydrolase protein variants and cofactors that enhance GH61 activity
US20130084608A1 (en) 2011-09-30 2013-04-04 Codexis, Inc. Fungal proteases
CA2851855A1 (en) 2011-10-31 2013-05-10 Bp Corporation North America Inc. Use of plant promoters in filamentous fungi
US20130109055A1 (en) 2011-10-31 2013-05-02 Bp Corporation North America Inc. Use of mammalian promoters in filamentous fungi
US20140234897A1 (en) * 2011-11-04 2014-08-21 Novozymes Inc. Polypeptides Having Cellobiohydrolase Activity and Polynucleotides Encoding Same
EP2794871A4 (de) 2011-12-20 2015-12-30 Codexis Inc Varianten von endoglucanase-1b (eg1b)
BR112014031026A2 (pt) 2012-06-11 2017-06-27 Codexis Inc variantes de beta-xilosidase fúngica.
US20150376590A1 (en) 2012-11-02 2015-12-31 Bp Corporation North America Inc. Thermotolerant Beta-Glucosidase Variants
WO2014145768A2 (en) 2013-03-15 2014-09-18 Bp Corporation North America Inc. Use of non-fungal 5' utrs in filamentous fungi
ES2521940B1 (es) 2013-05-10 2015-09-09 Abengoa Bioenergía Nuevas Tecnologías, S.A. Variantes de beta-glucosidasa con actividad de transglicosilación reducida
ES2727390T3 (es) 2013-05-20 2019-10-15 Abengoa Bioenergia Nuevas Tecnologias Sa Expresión de enzimas beta-xilosidasas recombinantes
ES2527368B1 (es) 2013-07-22 2015-10-27 Abengoa Bioenergía Nuevas Tecnologías, S.A. Célula hospedadora de Myceliophthora thermophila que expresa una enzima alfa-xilosidasa heteróloga y su uso en un procedimiento de degradación de biomasa
WO2015081139A1 (en) 2013-11-26 2015-06-04 Novozymes A/S Enzyme compositions and uses thereof
ES2542621B1 (es) 2014-02-07 2016-08-16 Abengoa Bioenergía Nuevas Tecnologías, S.A. Polipéptidos con actividad polisacárido monooxigenasa y su uso para la producción de azúcares fermentables
EP3152315B1 (de) 2014-06-06 2018-08-15 Novozymes A/S Enzymzusammensetzungen und ihre verwendungen
WO2016142536A1 (en) 2015-03-11 2016-09-15 Genencor International B.V. Enzymatic activity of lytic polysaccharide monooxygenase
US9944916B2 (en) 2015-05-07 2018-04-17 Codexis, Inc. Penicillin-G acylases
WO2016207351A2 (en) 2015-06-24 2016-12-29 Genencor International B.V. Polypeptides having demethylating activity
EP3330375A1 (de) 2015-07-29 2018-06-06 Abengoa Bioenergía Nuevas Tecnologías, S. A. Expression von rekombinanten beta-xylosidaseenzymen
JP2019503168A (ja) 2015-12-02 2019-02-07 ビーエーエスエフ ソシエタス・ヨーロピアBasf Se Clr2活性が低減した糸状菌においてタンパク質を生産する方法
BR112018010310A2 (pt) 2015-12-02 2018-12-04 Basf Se método de produção de polipeptídeos recombinantes, fungo filamentoso, uso de construção de ácido nucleico e uso de fungo filamentoso
CA3007635A1 (en) 2015-12-07 2017-06-15 Zymergen Inc. Promoters from corynebacterium glutamicum
US9988624B2 (en) 2015-12-07 2018-06-05 Zymergen Inc. Microbial strain improvement by a HTP genomic engineering platform
US11208649B2 (en) 2015-12-07 2021-12-28 Zymergen Inc. HTP genomic engineering platform
EP3419992B1 (de) 2016-02-22 2020-11-18 Danisco US Inc. Pilzsystem zur high-level-herstellung von proteinen
JP7128519B2 (ja) 2016-05-05 2022-08-31 コデクシス, インコーポレイテッド ペニシリンgアシラーゼ
ES2647322B1 (es) * 2016-05-20 2018-10-05 Abengoa Bioenergía Nuevas Tecnologías, S.A. Célula hospedadora Myceliophthora thermophila con actividad celulolítica mejorada y composiciones enzimáticas obtenidas por la misma
WO2018005225A1 (en) 2016-06-30 2018-01-04 Danisco Us Inc Aspartic proteases
US10544390B2 (en) 2016-06-30 2020-01-28 Zymergen Inc. Methods for generating a bacterial hemoglobin library and uses thereof
JP2019519241A (ja) 2016-06-30 2019-07-11 ザイマージェン インコーポレイテッド グルコース透過酵素ライブラリーを生成するための方法およびその使用
WO2018129130A1 (en) 2017-01-05 2018-07-12 Codexis, Inc. Penicillin-g acylases
DK3378936T3 (da) * 2017-03-24 2021-08-16 Clariant Int Ltd Cellulase, som er egnet til anvendelse i detergentsammensætninger
CA3061984A1 (en) 2017-06-06 2018-12-13 Zymergen Inc. A htp genomic engineering platform for improving fungal strains
EP3638688A4 (de) 2017-06-14 2021-07-07 Codexis, Inc. Manipulierte transaminase-polypeptide für industrielle biokatalyse
JP7244089B2 (ja) 2017-06-27 2023-03-22 コデクシス, インコーポレイテッド ペニシリンgアシラーゼ
EP3672630A4 (de) * 2017-08-21 2021-06-02 Dyadic International Inc. Herstellung eines grippeimpfstoffes in myceliophthora thermophila
CN111511389A (zh) 2017-11-07 2020-08-07 科德克希思公司 转谷氨酰胺酶变体
CN109988715B (zh) * 2017-12-29 2022-03-01 青岛蔚蓝生物集团有限公司 一种高产木聚糖酶的突变菌株及其应用
CN110093332B (zh) * 2018-01-30 2021-12-28 青岛蔚蓝生物集团有限公司 一种纤维素酶突变体及其高产菌株
CN112166180A (zh) 2018-06-06 2021-01-01 齐默尔根公司 操纵涉及信号转导的基因以控制发酵和生产期间的真菌形态
EP3821015A4 (de) 2018-07-09 2022-07-13 Codexis, Inc. Gentechnisch hergestellte desoxyribose-phosphat-aldolasen
CN108913713A (zh) * 2018-07-24 2018-11-30 中国农业科学院饲料研究所 提高里氏木霉转化效率的重组表达载体及应用
EP3636735B1 (de) 2018-10-12 2024-03-27 AB Enzymes Oy Variante einer protease und die verwendung davon
IL296841A (en) 2020-04-10 2022-11-01 Codexis Inc Transaminase transaminase polypeptides
WO2021220209A1 (en) 2020-04-30 2021-11-04 Dyadic International (Usa), Inc. Nicotinamide riboside production in filamentous fungi
US11479779B2 (en) 2020-07-31 2022-10-25 Zymergen Inc. Systems and methods for high-throughput automated strain generation for non-sporulating fungi
CN113403209B (zh) * 2021-07-30 2022-08-26 西南大学 天冬氨酸蛋白酶基因在改良球孢白僵菌菌株中的应用
CN121127575A (zh) 2022-12-19 2025-12-12 巴斯夫欧洲公司 用于真菌蛋白生产的新型纤维素酶启动子
CN120641568A (zh) 2023-01-27 2025-09-12 巴斯夫欧洲公司 用于真菌蛋白和精细化学品生产的新型基因组整合位点
WO2025262103A1 (en) 2024-06-19 2025-12-26 Basf Se Superior promoters for fungal protein production

Family Cites Families (111)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2974001A (en) 1957-07-20 1961-03-07 Kalle Ag Process and compositions for enzymatic desizing and bleaching of textiles
GB1368599A (en) 1970-09-29 1974-10-02 Unilever Ltd Softening compositions
JPS5028515B2 (de) 1971-09-30 1975-09-16
US3966543A (en) 1972-10-30 1976-06-29 Baxter Laboratories, Inc. Enzyme-treated paper
US4081328A (en) 1975-10-23 1978-03-28 Stanford Research Institute Production of cellulase by a thermophilic thielavia terrestris
DK187280A (da) 1980-04-30 1981-10-31 Novo Industri As Ruhedsreducerende middel til et fuldvaskemiddel fuldvaskemiddel og fuldvaskemetode
GB2094826B (en) 1981-03-05 1985-06-12 Kao Corp Cellulase enzyme detergent composition
JPS591598A (ja) 1982-06-25 1984-01-06 花王株式会社 洗浄剤組成物
US4486533A (en) 1982-09-02 1984-12-04 St. Louis University Filamentous fungi functional replicating extrachromosomal element
NL8300698A (nl) 1983-02-24 1984-09-17 Univ Leiden Werkwijze voor het inbouwen van vreemd dna in het genoom van tweezaadlobbige planten; agrobacterium tumefaciens bacterien en werkwijze voor het produceren daarvan; planten en plantecellen met gewijzigde genetische eigenschappen; werkwijze voor het bereiden van chemische en/of farmaceutische produkten.
GB8306645D0 (en) 1983-03-10 1983-04-13 Unilever Plc Detergent compositions
US4462307A (en) 1983-05-23 1984-07-31 Pet Incorporated Humpback oven-broiler
GB8421800D0 (en) 1984-08-29 1984-10-03 Unilever Plc Detergent compositions
GB8422238D0 (en) 1984-09-03 1984-10-10 Neuberger M S Chimeric proteins
US4816405A (en) 1984-10-24 1989-03-28 The Regents Of The University Of California Vectors for transformation by ascomycetes
US4885249A (en) 1984-12-05 1989-12-05 Allelix, Inc. Aspergillus niger transformation system
US4610800A (en) 1985-01-25 1986-09-09 Genex Corporation Method for unclogging drainage pipes
US6492107B1 (en) 1986-11-20 2002-12-10 Stuart Kauffman Process for obtaining DNA, RNA, peptides, polypeptides, or protein, by recombinant DNA technique
US5198345A (en) 1985-04-15 1993-03-30 Gist-Brocades N.V. Vectors in use in filamentous fungi
ATE117020T1 (de) * 1985-08-29 1995-01-15 Genencor Int Expression von heterologen polypeptiden in filamentösen pilzen, verfahren zu deren herstellung und vektoren zu deren herstellung.
US5364770A (en) 1985-08-29 1994-11-15 Genencor International Inc. Heterologous polypeptides expressed in aspergillus
DK163591C (da) 1985-10-08 1992-08-24 Novo Nordisk As Fremgangsmaade til behandling af et tekstilstof med en cellulase
US4935349A (en) 1986-01-17 1990-06-19 Zymogenetics, Inc. Expression of higher eucaryotic genes in aspergillus
US5766912A (en) 1986-03-17 1998-06-16 Novo Nordisk A/S Humicola lipase produced in aspergillus
US5536661A (en) 1987-03-10 1996-07-16 Novo Nordisk A/S Process for the production of protein products in aspergillus
GB8607679D0 (en) 1986-03-27 1986-04-30 Winter G P Recombinant dna product
US5252726A (en) 1987-09-04 1993-10-12 Novo Nordisk A/S Promoters for use in aspergillus
US4832864A (en) 1987-09-15 1989-05-23 Ecolab Inc. Compositions and methods that introduce variations in color density into cellulosic fabrics, particularly indigo dyed denim
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
US5122159A (en) 1988-09-15 1992-06-16 Ecolab Inc. Cellulase compositions and methods that introduce variations in color density into cellulosic fabrics, particularly indigo dyed denim
US5006126A (en) 1988-09-15 1991-04-09 Ecolab Inc. Cellulase compositions and methods that introduce variations in color density into cellulosic fabrics, particularly indigo dyed denim
IL162181A (en) 1988-12-28 2006-04-10 Pdl Biopharma Inc A method of producing humanized immunoglubulin, and polynucleotides encoding the same
US5571697A (en) 1989-05-05 1996-11-05 Baylor College Of Medicine Texas Medical Center Expression of processed recombinant lactoferrin and lactoferrin polypeptide fragments from a fusion product in Aspergillus
KR920702621A (ko) 1989-06-13 1992-10-06 스튜어트 알. 슈터 단핵세포 및/또는 마크로파지에 의한 인터루킨-1 또는 종양회사인자 생성의 억제
ES2150188T3 (es) 1989-07-07 2000-11-16 Unilever Nv Procedimiento para la preparacion de una proteina mediante un hongo transformado por integracion multicopia de un vector de expresion.
US5120463A (en) 1989-10-19 1992-06-09 Genencor International, Inc. Degradation resistant detergent compositions based on cellulase enzymes
GB8928874D0 (en) 1989-12-21 1990-02-28 Celltech Ltd Humanised antibodies
JPH05505308A (ja) 1990-03-13 1993-08-12 ハワイ・バイオテクノロジー・グループ・インコーポレイテツド アオパンカビ発現システム
DK115890D0 (da) 1990-05-09 1990-05-09 Novo Nordisk As Enzym
GB9015198D0 (en) 1990-07-10 1990-08-29 Brien Caroline J O Binding substance
PT98310B (pt) 1990-07-13 1999-01-29 Transkaryotic Therapies Inc Processo de recombinacao homologa para identificacao e isolamento dum fragmento pretendido de adn a partir de uma biblioteca de fragmentos
CA2090126C (en) 1990-08-02 2002-10-22 John W. Schrader Methods for the production of proteins with a desired function
JP3133774B2 (ja) 1990-10-04 2001-02-13 日本食品化工株式会社 新規な宿主−ベクター系
US5290474A (en) 1990-10-05 1994-03-01 Genencor International, Inc. Detergent composition for treating cotton-containing fabrics containing a surfactant and a cellulase composition containing endolucanase III from trichoderma ssp
US5780279A (en) 1990-12-03 1998-07-14 Genentech, Inc. Method of selection of proteolytic cleavage sites by directed evolution and phagemid display
JPH06503960A (ja) 1990-12-10 1994-05-12 ジェネンコア インターナショナル インコーポレーテッド TRICHODERMA REESEIのβ−グルコシダーゼ遺伝子のクローニングおよび増幅によるセルロースの改良糖化
GB9102635D0 (en) 1991-02-07 1991-03-27 British Bio Technology Compounds
US5858657A (en) 1992-05-15 1999-01-12 Medical Research Council Methods for producing members of specific binding pairs
US5871907A (en) 1991-05-15 1999-02-16 Medical Research Council Methods for producing members of specific binding pairs
ES2136092T3 (es) 1991-09-23 1999-11-16 Medical Res Council Procedimientos para la produccion de anticuerpos humanizados.
US5516670A (en) 1991-09-30 1996-05-14 Kuehnle; Adelheid R. Magnetophoretic particle delivery method and apparatus for the treatment of cells
BE1005432A4 (nl) 1991-10-01 1993-07-20 Dsm Nv Expressiecassette voor heterologe eiwitten.
JP2866739B2 (ja) 1991-12-09 1999-03-08 ユニリーバー・ナームローゼ・ベンノートシヤープ アスペルギルスエンドキシラナーゼ▲ii▼遺伝子に由来する発現/分泌調節領域を用い、形質転換したカビでタンパク質を産生して分泌させるための方法
CA2062023A1 (en) 1992-02-10 1993-08-11 Jagroop S. Dahiya Novel fungal strains and use thereof in antibiotic production
EP0656943A1 (de) 1992-08-19 1995-06-14 Alko Group Ltd. In der gegenwart von glukose aktiver promotor aus pilzzellen
EP0663953B1 (de) 1992-09-04 2001-12-05 The Scripps Research Institute Phagemiden die einen oberflächenrezeptor und ein heterologes oberflächenprotein co-exprimieren
CA2151154C (en) 1992-12-10 1999-01-26 William E. Hintz Production of heterologous proteins in filamentous fungi
GB9316883D0 (en) 1993-08-13 1993-09-29 Univ Leeds Production of heterologous peptices
US5849541A (en) 1993-11-02 1998-12-15 Merck & Co., Inc. DNA encoding triol polyketide synthase
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5820866A (en) 1994-03-04 1998-10-13 National Jewish Center For Immunology And Respiratory Medicine Product and process for T cell regulation
GB2289218A (en) 1994-05-06 1995-11-15 Merck & Co Inc Inhibition of TNFalpha production with agonists of the A2b subtype of the adenosine receptor
US6025185A (en) 1994-06-17 2000-02-15 Novo Nordisk Als Fungus wherein the areA gene has been modified and an areA gene from Aspergillus oryzae
US6060305A (en) 1994-06-30 2000-05-09 Novo Nordisk Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic Fusarium expression system
ATE294871T1 (de) 1994-06-30 2005-05-15 Novozymes Biotech Inc Nicht-toxisches, nicht-toxigenes, nicht- pathogenes fusarium expressionssystem und darin zu verwendende promotoren und terminatoren
EP0770090A1 (de) 1994-07-13 1997-05-02 Cornell Research Foundation, Inc. Protein des epstein-barr virus kernantigen 1 und seine expression und anreicherung
US5602004A (en) 1994-07-20 1997-02-11 Novo Nordisk Biotech, Inc. Thermophilic fungal expression system
CN1225550C (zh) 1995-03-20 2005-11-02 诺沃奇梅兹有限公司 表达降低水平的金属蛋白酶的宿主细胞和在蛋白质产生中使用该宿主细胞的方法
US6046021A (en) 1995-04-12 2000-04-04 Biolog, Inc. Comparative phenotype analysis of two or more microorganisms using a plurality of substrates within a multiwell testing device
AU723619B2 (en) 1995-04-24 2000-08-31 Chromaxome Corporation Methods for generating and screening novel metabolic pathways
US6030779A (en) 1995-07-18 2000-02-29 Diversa Corporation Screening for novel bioactivities
US5958672A (en) 1995-07-18 1999-09-28 Diversa Corporation Protein activity screening of clones having DNA from uncultivated microorganisms
US6057103A (en) 1995-07-18 2000-05-02 Diversa Corporation Screening for novel bioactivities
FI103133B1 (fi) 1995-09-01 1999-04-30 Valtion Teknillinen Menetelmä glukoosirepression modifioimiseksi
AU7294396A (en) 1995-10-13 1997-04-30 Gist-Brocades B.V. Protein detection
US5830696A (en) 1996-12-05 1998-11-03 Diversa Corporation Directed evolution of thermophilic enzymes
US5939250A (en) 1995-12-07 1999-08-17 Diversa Corporation Production of enzymes having desired activities by mutagenesis
US5776730A (en) 1995-12-15 1998-07-07 University Of Hawaii Neurospora hosts for the production of recombinant proteins, and methods for producing same
EP2295581A3 (de) 1996-01-19 2011-05-04 Novozymes Biotech, Inc. Morphologische Mutanten von filamentösen Pilzen
EP0876534B1 (de) 1996-01-26 2001-05-16 Novozymes A/S Herstellung von toilettenpapier
US5753477A (en) 1996-03-19 1998-05-19 University Technology Corporation Magneto-biolistic methods
US5783431A (en) 1996-04-24 1998-07-21 Chromaxome Corporation Methods for generating and screening novel metabolic pathways
US5811381A (en) * 1996-10-10 1998-09-22 Mark A. Emalfarb Cellulase compositions and methods of use
US7883872B2 (en) 1996-10-10 2011-02-08 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
US5879921A (en) 1996-11-07 1999-03-09 Novo Nordisk A/S Recombinant expression of a glucose oxidase from a cladosporium strain
DE69738768D1 (de) 1996-12-13 2008-07-24 Novartis Vaccines & Diagnostic Analyse und separation von pdgf-proteinen
US5989814A (en) 1997-04-01 1999-11-23 Reagents Of The University Of California Screening methods in eucaryotic cells
US6573068B1 (en) 1997-11-13 2003-06-03 Genset, S. A. Claudin-50 protein
ES2143408B1 (es) 1998-04-02 2000-12-01 Urquima Sa Promotor y construcciones para expresion de proteinas recombinantes en hongos filamentosos.
US5914245A (en) 1998-04-20 1999-06-22 Kairos Scientific Inc. Solid phase enzyme kinetics screening in microcolonies
US6413776B1 (en) 1998-06-12 2002-07-02 Galapagos Geonomics N.V. High throughput screening of gene function using adenoviral libraries for functional genomics applications
WO1999067639A1 (en) 1998-06-25 1999-12-29 Caliper Technologies Corporation High throughput methods, systems and apparatus for performing cell based screening assays
EP1092039B1 (de) 1998-06-29 2012-02-29 Bristol-Myers Squibb Company Verfahren zur erzeugung von hochgradig diversen bibliotheken
KR100618495B1 (ko) 1998-10-06 2006-08-31 마크 아론 에말파브 섬유상의 진균성 숙주 영역에서의 형질전환 시스템:크리소스포륨속에서
US6518042B1 (en) 1999-02-24 2003-02-11 Novozymes A/S Process for making DNA libraries in filamentous fungal cells using a novel cloned gene involved in the mismatch repair system of filamentous fungal cells
EP1194572A2 (de) 1999-03-22 2002-04-10 Novozymes Biotech, Inc. Promotorsequenzen von fusarium venenatum und deren verwendungen
US6121034A (en) 1999-05-13 2000-09-19 Her Majesty The Queen In Right Of Canada, As Represented By The Minister Of Agriculture And Agri-Food Coniothyrium minitans xylanase gene Cxy1
AU4352700A (en) 1999-10-06 2001-05-10 Mark Aaron Emalfarb High-throughput screening of expressed dna libraries in filamentous fungi
WO2001079558A1 (en) 2000-04-13 2001-10-25 Mark Aaron Emalfarb High-throughput screening of expressed dna libraries in filamentous fungi
US7122330B2 (en) 2000-04-13 2006-10-17 Mark Aaron Emalfarb High-throughput screening of expressed DNA libraries in filamentous fungi
HUP0300469A2 (hu) 2000-04-13 2003-06-28 Emalfarb, Mark Aaron Új, expressziós szabályzó szekvenciák és expressziós termékek fonalas gombákban
AU2002243972A1 (en) 2001-02-13 2002-08-28 Diadexus, Inc. Compositions and methods relating to breast specific genes and proteins
US7205110B2 (en) 2002-10-07 2007-04-17 Zymogenetics, Inc. Uses of human Zven antagonists
DE60328715D1 (de) 2002-12-20 2009-09-17 Novozymes As Polypeptide mit cellobiohydrolase ii-aktivität und dafür kodierende polynucleotide
ES2472669T3 (es) 2004-01-30 2014-07-02 Novozymes Inc. Polipéptidos con actividad de mejora celulolítica y polinucleótidos que los codifican
CA2554784C (en) 2004-02-06 2013-05-28 Novozymes, Inc. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
US7332319B2 (en) 2004-05-27 2008-02-19 Genencor International, Inc. Heterologous alpha amylase expression in Aspergillus
US20060105361A1 (en) 2004-08-24 2006-05-18 Rothstein Rodney J Donor yeast strain for transfer of genetic material
CA2593246C (en) 2005-01-06 2016-06-21 Novozymes, Inc. Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
CN101321857A (zh) 2005-09-30 2008-12-10 诺维信股份有限公司 用于增强纤维素材料降解或转化的方法
US7923236B2 (en) 2007-08-02 2011-04-12 Dyadic International (Usa), Inc. Fungal enzymes

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US20080194005A1 (en) 2008-08-14
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CA2345356A1 (en) 2000-04-13
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BR9914278A (pt) 2001-06-19
US8268585B2 (en) 2012-09-18
AU6232699A (en) 2000-04-26
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US6573086B1 (en) 2003-06-03
AU771539B2 (en) 2004-03-25
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BRPI9914278B1 (pt) 2016-03-08
US20140127788A1 (en) 2014-05-08

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