DE241435T1 - Vektoren fuer die klonierung und expression heterologer gene in hefen und durch diese vektoren transformierte hefestaemme. - Google Patents

Vektoren fuer die klonierung und expression heterologer gene in hefen und durch diese vektoren transformierte hefestaemme.

Info

Publication number
DE241435T1
DE241435T1 DE198787830114T DE87830114T DE241435T1 DE 241435 T1 DE241435 T1 DE 241435T1 DE 198787830114 T DE198787830114 T DE 198787830114T DE 87830114 T DE87830114 T DE 87830114T DE 241435 T1 DE241435 T1 DE 241435T1
Authority
DE
Germany
Prior art keywords
vectors
gene
expression
vector according
plasmid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
DE198787830114T
Other languages
English (en)
Inventor
Falcone I-00197 Rome Claudio
Fukuhara F-91190 Gif Sur Yvette Hiroshi
Frontali I-00198 Laura
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique CNRS
Consiglio Nazionale delle Richerche CNR
Universita degli Studi di Roma La Sapienza
Original Assignee
Centre National de la Recherche Scientifique CNRS
Consiglio Nazionale delle Richerche CNR
Universita degli Studi di Roma La Sapienza
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre National de la Recherche Scientifique CNRS, Consiglio Nazionale delle Richerche CNR, Universita degli Studi di Roma La Sapienza filed Critical Centre National de la Recherche Scientifique CNRS
Publication of DE241435T1 publication Critical patent/DE241435T1/de
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Claims (9)

Europäische Patentanmeldung Nr. 87830114.2 12530 Dr.v.B/Ri PATENTANSPRÜCHE
1. Klonungs- und Expressionsvektoren für heterologe Gene in Hefen, dadurch gekennzeichnet/ daß diese Vehikel zumindest die ganze DNA des pKD1-Plasmids (isoliert aus Kluyveromyces drosophilarum) enthalten oder einen Teil hiervon, und ein DNA-Segment, das irgendein heterologes Gen trägt, einschließlich Sequenzen, die die Expression dieses Gens gewährleisten.
2. Vektor nach Anspruch 1, bei dem das heterologe Gen in eine der einmaligen Restriktionsstellen des pKD1-Plasmids eingesetzt ist.
3. Vektor nach Anspruch 2, dadurch gekennzeichnet, daß
die einmalige Restriktionsstelle, die Pstl-Stelle ist.
4. Vektor nach Anspruch 1-3, dadurch gekennzeichnet, daß das heterologe Gen das URA3-Gen von Saccharomyces cerevisiae ist.
5. Vektor nach Anspruch 4, dadurch gekennzeichnet, daß das DNA-Segment, das das üRA3-Gen trägt, die DNA des YIP5-Plasmids ist.
6. Vektor nach Anspruch 5, der ein 1,7 Kilobasen langes DNA-Fragment des pKD1-Plasmids enthält, welches Fragment mit dem Enzym BamHI erhalten und an der einmaligen BamHI-Stelle des YIP5-Plasmids eingesetzt ist.
7. Hefen, die durch die in den Ansprüchen 1->6 bezeichneten Vektoren transformiert sind.
8. Hefen nach Anspruch 7, die zur Gattung Kluyveromyces gehören.
9. Hefen nach Anspruch 8, die zur Art Kluyveromyces lactis gehören.
DE198787830114T 1986-03-27 1987-03-25 Vektoren fuer die klonierung und expression heterologer gene in hefen und durch diese vektoren transformierte hefestaemme. Pending DE241435T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IT47830/86A IT1203758B (it) 1986-03-27 1986-03-27 Vettori di clonazione e di espressione di geni eterologhi in lieviti e lieviti trasformati con tali vettori

Publications (1)

Publication Number Publication Date
DE241435T1 true DE241435T1 (de) 1989-03-09

Family

ID=11262795

Family Applications (2)

Application Number Title Priority Date Filing Date
DE198787830114T Pending DE241435T1 (de) 1986-03-27 1987-03-25 Vektoren fuer die klonierung und expression heterologer gene in hefen und durch diese vektoren transformierte hefestaemme.
DE8787830114T Expired - Lifetime DE3782844T2 (de) 1986-03-27 1987-03-25 Vektoren fuer die klonierung und expression heterologer gene in hefen und durch diese vektoren transformierte hefestaemme.

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE8787830114T Expired - Lifetime DE3782844T2 (de) 1986-03-27 1987-03-25 Vektoren fuer die klonierung und expression heterologer gene in hefen und durch diese vektoren transformierte hefestaemme.

Country Status (7)

Country Link
EP (1) EP0241435B1 (de)
JP (1) JP2646085B2 (de)
AT (1) ATE83009T1 (de)
DE (2) DE241435T1 (de)
ES (1) ES2053586T3 (de)
GR (1) GR3007153T3 (de)
IT (1) IT1203758B (de)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4943529A (en) 1982-05-19 1990-07-24 Gist-Brocades Nv Kluyveromyces as a host strain
NZ225597A (en) * 1987-07-28 1991-08-27 Gist Brocades Nv Use of kluyveromyces in the production of chymosin, tpa, hsa and other proteins
FR2649991B2 (fr) 1988-08-05 1994-03-04 Rhone Poulenc Sante Utilisation de derives stables du plasmide pkd1 pour l'expression et la secretion de proteines heterologues dans les levures du genre kluyveromyces
JPH0486191A (ja) * 1990-07-30 1992-03-18 Aiphone Co Ltd テレビドアホン方式
FR2678636A1 (fr) * 1991-07-02 1993-01-08 Rhone Poulenc Rorer Sa Procede de production de proteines recombinantes et cellules hote utilisees.
FR2686899B1 (fr) 1992-01-31 1995-09-01 Rhone Poulenc Rorer Sa Nouveaux polypeptides biologiquement actifs, leur preparation et compositions pharmaceutiques les contenant.
DE4244915C2 (de) * 1992-08-14 1998-12-03 Widmar Prof Dr Tanner DNA-Moleküle codierend Proteine, die an der O-Glykosylierung von Proteinen beteiligt sind
GB9526733D0 (en) 1995-12-30 1996-02-28 Delta Biotechnology Ltd Fusion proteins
US6358705B1 (en) 1998-07-16 2002-03-19 Novo Nordisk A/S Method of making proteins in transformed yeast cells
US6946134B1 (en) 2000-04-12 2005-09-20 Human Genome Sciences, Inc. Albumin fusion proteins
EP2067488A1 (de) 2000-04-12 2009-06-10 Human Genome Sciences, Inc. Albuminfusionsproteine
MXPA04003333A (es) 2001-10-10 2006-02-22 Neose Technologies Inc Remodelado y glicoconjugacion de peptidos.
EP2298354B1 (de) 2001-10-10 2014-03-19 ratiopharm GmbH Neumodulierung und Glykokonjugation von Interferon-beta
ES2500918T3 (es) 2001-12-21 2014-10-01 Human Genome Sciences, Inc. Proteínas de fusión de albúmina e interferón beta
CA2484556A1 (en) 2001-12-21 2003-07-24 Human Genome Sciences, Inc. Albumin fusion proteins
US20070026485A1 (en) 2003-04-09 2007-02-01 Neose Technologies, Inc. Glycopegylation methods and proteins/peptides produced by the methods

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0096910B1 (de) * 1982-05-19 1989-04-19 Unilever N.V. Hefe des Genus Kluyveromyces, modifiziert zur Expression von Preprothaumatin oder seinen verschiedenen allelischen und modifizierten Formen oder Maturationsformen
FR2532656B2 (fr) * 1982-06-02 1985-10-18 Elf Bio Rech Nouveau vecteur de clonage et d'expression, levure et bacterie transformees par ce vecteur

Also Published As

Publication number Publication date
ATE83009T1 (de) 1992-12-15
EP0241435A2 (de) 1987-10-14
EP0241435A3 (en) 1988-09-14
IT8647830A0 (it) 1986-03-27
ES2053586T3 (es) 1994-08-01
IT1203758B (it) 1989-02-23
JPS63290A (ja) 1988-01-05
DE3782844T2 (de) 1993-06-17
GR3007153T3 (de) 1993-07-30
JP2646085B2 (ja) 1997-08-25
EP0241435B1 (de) 1992-12-02
DE3782844D1 (de) 1993-01-14

Similar Documents

Publication Publication Date Title
DE241435T1 (de) Vektoren fuer die klonierung und expression heterologer gene in hefen und durch diese vektoren transformierte hefestaemme.
FI854145A0 (fi) Pichia-sukuisten hiivojen transformointi
DE3578435D1 (de) Promotoren fuer die expression von fremden genen in hefe, plasmide, die diese promotoren enthalten, sowie deren verwendung zur herstellung von polypeptiden.
Sato et al. Identification and characterization of genes induced during sexual differentiation in Schizosaccharomyces pombe
NAUMOV et al. Two new genetically isolated populations of the Saccharomyces sensu stricto complex from Japan
ATE246249T1 (de) Für ammonium-transporter kodierende dna-sequenzen und dieser enthaltende, plasmide, bakterien, hefen und pflanzenzellen
Ikeuchi et al. Identities of four low‐molecular‐mass subunits of the photosystem I complex from Anabaena variabilis ATCC 29413: Evidence for the presence of the psaI gene product in a cyanobacterial complex
IL155940A0 (en) Promoters exhibiting endothelial cell specificity and method of using same
Wang et al. Protein specificity and sexual morphogenesis in Schizophyllum commune
DE205506T1 (de) Verfahren zur transformation von pilzen mit einem vektor.
ES8802251A1 (es) Un procedimiento para preparar un microorganismo hospedante transformado del genero sacharomyces.
Van der Westhuizen et al. The value of long-chain fatty acid analysis, randomly amplified polymorphic DNA and electrophoretic karyotyping for the characterization of wine yeast strains
Kurischko et al. Development of breeding stocks of the yeast Saccharomycopsis lipolytica by methods of moderate inbreeding
Nagahora et al. Alterations in the cleavage site of the signal sequence for the secretion of human lysozyme by Saccharomyces cerevisiae
ATE50453T1 (de) Spezifisches polypeptid produzierende hybridzellinien, erhaeltlich aus primaerzellen, die spontan faehig sind zur expres sion dieses polypeptids oder eines aehnlichen polypeptids, verfahren zum erhalten dieser hybridzellen und deren verwendung zur herstellung dieses polypeptids.
WO2000028054A3 (en) Plant proteins that interact with nuclear matrix proteins and function as transcriptional activators
Blank et al. Effects of previous order and proportion of similar attitude statements on attraction during a subsequent series of dissimilar statements
Guerineau Selected topics from the congress on the Genetics Industrial Microorganisms (GIM): from genetic plasticity to edible mushrooms
Epton Microbial Control of Weeds, David O. TeBeest (Ed.), Chapman & Hall, London (1991)
Ajam et al. THE TIMING OF SPECIFIC PROTEIN CHANGES DURING YEAST SPORULATION
WO2001040512A3 (en) Resistance gene
NUNN THE GENETICS OF METHANOL OXIDATION IN THE FACULTATIVE METHYLOTROPH METHYLOBACTERIUM AM1.
Van Heeckeren Two studies related to transcriptional regulation in the budding yeast and the fission yeast.
Shibata TORSIONAL DYNAMICS OF LINEAR AND CIRCULAR DNAS.
DE86649T1 (de) Molekulare klonierung und charakterisierung der fuer das schweine-relaxin kodierenden gen-sequenz.