ATE398181T1 - Hefezellen welche zumindest zwei kopien von einem erwünschten gens enthalt und integrierte in dem chromosomal genom auf mehr als einem nicht- ribosomaler rns kodierende bereich, ins besonders mit kluyveromyces - Google Patents

Hefezellen welche zumindest zwei kopien von einem erwünschten gens enthalt und integrierte in dem chromosomal genom auf mehr als einem nicht- ribosomaler rns kodierende bereich, ins besonders mit kluyveromyces

Info

Publication number
ATE398181T1
ATE398181T1 AT98928202T AT98928202T ATE398181T1 AT E398181 T1 ATE398181 T1 AT E398181T1 AT 98928202 T AT98928202 T AT 98928202T AT 98928202 T AT98928202 T AT 98928202T AT E398181 T1 ATE398181 T1 AT E398181T1
Authority
AT
Austria
Prior art keywords
desired gene
copies
ribosomal rna
yeast cells
kluyveromyces
Prior art date
Application number
AT98928202T
Other languages
English (en)
Inventor
Bart Swinkels
OOIJEN Albert VAN
Der Haak Adria Noordermeer-Van
Original Assignee
Dsm Ip Assets Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Ip Assets Bv filed Critical Dsm Ip Assets Bv
Application granted granted Critical
Publication of ATE398181T1 publication Critical patent/ATE398181T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AT98928202T 1997-04-11 1998-04-14 Hefezellen welche zumindest zwei kopien von einem erwünschten gens enthalt und integrierte in dem chromosomal genom auf mehr als einem nicht- ribosomaler rns kodierende bereich, ins besonders mit kluyveromyces ATE398181T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP97201053 1997-04-11
EP97201637 1997-06-03

Publications (1)

Publication Number Publication Date
ATE398181T1 true ATE398181T1 (de) 2008-07-15

Family

ID=26146351

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98928202T ATE398181T1 (de) 1997-04-11 1998-04-14 Hefezellen welche zumindest zwei kopien von einem erwünschten gens enthalt und integrierte in dem chromosomal genom auf mehr als einem nicht- ribosomaler rns kodierende bereich, ins besonders mit kluyveromyces

Country Status (8)

Country Link
EP (1) EP0973918B1 (de)
JP (1) JP4531868B2 (de)
AT (1) ATE398181T1 (de)
AU (1) AU8013798A (de)
DE (1) DE69839602D1 (de)
DK (1) DK0973918T3 (de)
ES (1) ES2308805T3 (de)
WO (1) WO1998046774A1 (de)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102597241B (zh) 2009-07-22 2015-09-02 帝斯曼知识产权资产管理有限公司 用于生产感兴趣的化合物的经改进的宿主细胞
AU2018241920B2 (en) 2017-03-29 2022-04-21 Boehringer Ingelheim Rcv Gmbh & Co Kg Recombinant host cell with altered membrane lipid composition

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IE55817B1 (en) * 1982-05-19 1991-01-30 Gist Brocades Nv Cloning system for kluyveromyces species
CA2063592C (en) * 1989-07-07 2003-01-21 Marco L. F. Giuseppin Process for preparing a protein by a fungus transformed by multicopy integration of an expression vector
IT1251435B (it) * 1991-09-04 1995-05-09 Sclavo Spa Vettore genetico per la integrazione multipla stabile di sequenze di dna nel genoma dei lieviti kluyveromyces lactis e saccharomyces cerevisiae e plasmidi che lo contengono.
DE69432543T2 (de) * 1993-07-23 2003-12-24 Dsm Nv Selektionmarker-genfreie rekombinante Stämme: Verfahren zur ihrer Herstellung und die Verwendung dieser Stämme

Also Published As

Publication number Publication date
DE69839602D1 (de) 2008-07-24
JP2002514077A (ja) 2002-05-14
EP0973918A1 (de) 2000-01-26
EP0973918B1 (de) 2008-06-11
DK0973918T3 (da) 2008-09-22
AU8013798A (en) 1998-11-11
JP4531868B2 (ja) 2010-08-25
WO1998046774A1 (en) 1998-10-22
ES2308805T3 (es) 2008-12-01

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