DE69839602D1 - Hefezellen welche zumindest zwei kopien von einem erwünschten gens enthalt und integrierte in dem chromosomal genom auf mehr als einem nicht-ribosomaler rns kodierende bereich, ins besonders mit kluyveromyces - Google Patents

Hefezellen welche zumindest zwei kopien von einem erwünschten gens enthalt und integrierte in dem chromosomal genom auf mehr als einem nicht-ribosomaler rns kodierende bereich, ins besonders mit kluyveromyces

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Publication number
DE69839602D1
DE69839602D1 DE69839602T DE69839602T DE69839602D1 DE 69839602 D1 DE69839602 D1 DE 69839602D1 DE 69839602 T DE69839602 T DE 69839602T DE 69839602 T DE69839602 T DE 69839602T DE 69839602 D1 DE69839602 D1 DE 69839602D1
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DE
Germany
Prior art keywords
copies
ribosomal rna
integrated
chromosomal
genomity
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
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DE69839602T
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English (en)
Inventor
Bart Willem Swinkels
Ooijen Albert Johannes Van
Der Haak Adria Noordermeer-Van
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DSM IP Assets BV
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DSM IP Assets BV
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
DE69839602T 1997-04-11 1998-04-14 Hefezellen welche zumindest zwei kopien von einem erwünschten gens enthalt und integrierte in dem chromosomal genom auf mehr als einem nicht-ribosomaler rns kodierende bereich, ins besonders mit kluyveromyces Expired - Lifetime DE69839602D1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP97201053 1997-04-11
EP97201637 1997-06-03
PCT/EP1998/002261 WO1998046774A1 (en) 1997-04-11 1998-04-14 Yeast cells comprising at least two copies of a desired gene integrated into the chromosomal genome at more than one non-ribosomal rna encoding domain, particularly with kluyveromyces

Publications (1)

Publication Number Publication Date
DE69839602D1 true DE69839602D1 (de) 2008-07-24

Family

ID=26146351

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69839602T Expired - Lifetime DE69839602D1 (de) 1997-04-11 1998-04-14 Hefezellen welche zumindest zwei kopien von einem erwünschten gens enthalt und integrierte in dem chromosomal genom auf mehr als einem nicht-ribosomaler rns kodierende bereich, ins besonders mit kluyveromyces

Country Status (8)

Country Link
EP (1) EP0973918B1 (de)
JP (1) JP4531868B2 (de)
AT (1) ATE398181T1 (de)
AU (1) AU8013798A (de)
DE (1) DE69839602D1 (de)
DK (1) DK0973918T3 (de)
ES (1) ES2308805T3 (de)
WO (1) WO1998046774A1 (de)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2010275672A1 (en) 2009-07-22 2012-02-02 Dsm Ip Assets B.V. Improved host cell for the production of a compound of interest
CN110612352A (zh) * 2017-03-29 2019-12-24 贝林格尔·英格海姆Rcv两合公司 具有改变的膜脂组成的重组宿主细胞

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2608540B2 (ja) * 1982-05-19 1997-05-07 ギスト ブロカデス ナ−ムロ−ゼ フエンノ−トチヤツプ クルイベロミセス種のためのクローニング系
JP3273609B2 (ja) * 1989-07-07 2002-04-08 ユニリーバー・ナームローゼ・ベンノートシヤープ 発現ベクターのマルチコピー組込みにより形質転換した真菌による蛋白質の製法
IT1251435B (it) * 1991-09-04 1995-05-09 Sclavo Spa Vettore genetico per la integrazione multipla stabile di sequenze di dna nel genoma dei lieviti kluyveromyces lactis e saccharomyces cerevisiae e plasmidi che lo contengono.
ES2198410T3 (es) * 1993-07-23 2004-02-01 Dsm Ip Assets B.V. Cepas recombinantes de hongos filamentosos libres de gen marcador de seleccion: un metodo para obtener dichas cepas y su utilizacion.

Also Published As

Publication number Publication date
EP0973918A1 (de) 2000-01-26
JP2002514077A (ja) 2002-05-14
ES2308805T3 (es) 2008-12-01
WO1998046774A1 (en) 1998-10-22
ATE398181T1 (de) 2008-07-15
DK0973918T3 (da) 2008-09-22
EP0973918B1 (de) 2008-06-11
JP4531868B2 (ja) 2010-08-25
AU8013798A (en) 1998-11-11

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DE69839602D1 (de) Hefezellen welche zumindest zwei kopien von einem erwünschten gens enthalt und integrierte in dem chromosomal genom auf mehr als einem nicht-ribosomaler rns kodierende bereich, ins besonders mit kluyveromyces
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