MX165920B - Un procedimiento para producir glucoamilasa - Google Patents

Un procedimiento para producir glucoamilasa

Info

Publication number
MX165920B
MX165920B MX000956A MX95685A MX165920B MX 165920 B MX165920 B MX 165920B MX 000956 A MX000956 A MX 000956A MX 95685 A MX95685 A MX 95685A MX 165920 B MX165920 B MX 165920B
Authority
MX
Mexico
Prior art keywords
procedure
glucoamylase
rhizopus
gene
microorganism
Prior art date
Application number
MX000956A
Other languages
English (en)
Inventor
Wuki Shigano
Hajime Yoshizumi
Toshihiko Ahikani
Norihisa Nakamura
Yoshikazu Tanaka
Original Assignee
Suntory Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Suntory Ltd filed Critical Suntory Ltd
Publication of MX165920B publication Critical patent/MX165920B/es

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • C12N9/2428Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/8215Microorganisms
    • Y10S435/911Microorganisms using fungi
    • Y10S435/94Saccharomyces
    • Y10S435/942Saccharomyces cerevisiae

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La presente invención se refiere a un procedimiento para producir glucoamilasa del género de Rhizopus, capaz de la hidrolisis eficiente del almidón crudo, dicho procedimiento caracterizado por las etapas de producir tal glucoamilasa por el cultivo de la levadura, E. coli cepa WA802 o Bacilus subtilis en un medio líquido y recuperar la glucoamilasa desde el cultivo, dicha levadura siendo una que se ha transformado por un vector recombinante el cual es capaz de expresión y que comprende un gene estructural de glucoamilasa derivado de un hongo del género Rhizopus.
MX000956A 1984-12-15 1985-12-16 Un procedimiento para producir glucoamilasa MX165920B (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP59264964A JPH0630586B2 (ja) 1984-12-15 1984-12-15 グルコアミラ−ゼ遺伝子

Publications (1)

Publication Number Publication Date
MX165920B true MX165920B (es) 1992-12-10

Family

ID=17410646

Family Applications (1)

Application Number Title Priority Date Filing Date
MX000956A MX165920B (es) 1984-12-15 1985-12-16 Un procedimiento para producir glucoamilasa

Country Status (8)

Country Link
US (1) US4863864A (es)
EP (1) EP0186066B1 (es)
JP (1) JPH0630586B2 (es)
AT (1) ATE86663T1 (es)
BR (1) BR8506273A (es)
CA (1) CA1267372A (es)
DE (1) DE3587168T2 (es)
MX (1) MX165920B (es)

Families Citing this family (53)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61141890A (ja) * 1984-12-15 1986-06-28 Suntory Ltd アルコ−ルの製造法
US6004785A (en) * 1985-08-29 1999-12-21 Genencor International Inc. Heterologous polypeptides expressed in filamentous fungi, processes for making same, and vectors for making same
US5364770A (en) * 1985-08-29 1994-11-15 Genencor International Inc. Heterologous polypeptides expressed in aspergillus
US5766912A (en) * 1986-03-17 1998-06-16 Novo Nordisk A/S Humicola lipase produced in aspergillus
US5536661A (en) * 1987-03-10 1996-07-16 Novo Nordisk A/S Process for the production of protein products in aspergillus
FR2599755B1 (fr) * 1986-06-10 1990-04-20 Transgene Sa Bloc d'expression d'une amyloglucosidase dans une levure, levure transformee et procede de preparation d'enzyme
JP2607509B2 (ja) * 1987-03-31 1997-05-07 サントリー株式会社 グルコアミラーゼ生産形質転換酵母によるアルコールの生産方法
US5604128A (en) * 1991-08-13 1997-02-18 Sankyo Company, Limited Isolated cultures of Pestalotiopsis funerea IFO 5427 and Pestalotiopsis negleta FERM BP-3501
US5665585A (en) * 1992-09-03 1997-09-09 Alko-Yhiot Oy Recombinant production of glucoamylase P in trichoderma
AU2446997A (en) * 1996-04-10 1997-10-29 Merck & Co., Inc. Method for improving culture medium for recombinant yeasts
US6232111B1 (en) 1996-04-10 2001-05-15 Merck & Co., Inc. Method for improving culture medium for recombinant yeast
CN1780560B (zh) * 2003-03-10 2011-05-25 布罗因联合公司 利用生淀粉生产乙醇的方法
US20050233030A1 (en) 2004-03-10 2005-10-20 Broin And Associates, Inc. Methods and systems for producing ethanol using raw starch and fractionation
CN1875099B (zh) * 2003-11-21 2011-05-04 金克克国际有限公司 颗粒淀粉水解酶在木霉菌中的表达和从颗粒淀粉底物产生葡萄糖的方法
US20050239181A1 (en) * 2004-03-10 2005-10-27 Broin And Associates, Inc. Continuous process for producing ethanol using raw starch
US7332319B2 (en) * 2004-05-27 2008-02-19 Genencor International, Inc. Heterologous alpha amylase expression in Aspergillus
WO2005117756A2 (en) * 2004-05-27 2005-12-15 Genencor International, Inc. Acid-stable alpha amylases having granular starch hydrolyzing activity and enzyme compositions
US7037704B2 (en) * 2004-05-27 2006-05-02 Genencor International, Inc. Heterologous expression of an Aspergillus kawachi acid-stable alpha amylase and applications in granular starch hydrolysis
US7413887B2 (en) * 2004-05-27 2008-08-19 Genecor International, Inc. Trichoderma reesei glucoamylase and homologs thereof
WO2006060062A2 (en) 2004-11-30 2006-06-08 Genencor International, Inc. Trichoderma reesei glucoamylase and homologs thereof
JP5087407B2 (ja) * 2004-12-30 2012-12-05 ジェネンコー・インターナショナル・インク 酸性真菌プロテアーゼ
US7662918B2 (en) * 2005-03-03 2010-02-16 Simpson Biotech Co., Ltd. Recombinant protein comprising starch binding domain and use thereof
US20070037267A1 (en) * 2005-05-02 2007-02-15 Broin And Associates, Inc. Methods and systems for producing ethanol using raw starch and fractionation
US7919289B2 (en) * 2005-10-10 2011-04-05 Poet Research, Inc. Methods and systems for producing ethanol using raw starch and selecting plant material
US7968318B2 (en) * 2006-06-06 2011-06-28 Genencor International, Inc. Process for conversion of granular starch to ethanol
US20080053157A1 (en) * 2006-08-31 2008-03-06 Anne Capelli Golding Knit Gloves
US9447397B2 (en) 2006-10-10 2016-09-20 Danisco Us Inc. Glucoamylase variants with altered properties
US8592194B2 (en) 2007-10-09 2013-11-26 Danisco Us Inc. Glucoamylase variants with altered properties
PL2514818T3 (pl) 2007-10-09 2014-11-28 Danisco Us Inc Odmiany glukoamylazy
ES2527586T3 (es) 2007-11-20 2015-01-27 Danisco Us Inc. Variantes de glucoamilasa con propiedades modificadas
AU2009256456B2 (en) * 2008-05-29 2012-11-08 Danisco Us Inc. Process for alcohol and co-product production from grain sorghum
US8450094B1 (en) 2009-03-03 2013-05-28 Poet Research, Inc. System for management of yeast to facilitate the production of ethanol
US9068206B1 (en) 2009-03-03 2015-06-30 Poet Research, Inc. System for treatment of biomass to facilitate the production of ethanol
MX2011009269A (es) 2009-03-03 2011-09-26 Poet Res Inc Fermentacion de biomasa para la produccion de etanol.
US20100233771A1 (en) * 2009-03-03 2010-09-16 Mcdonald William F System for pre-treatment of biomass for the production of ethanol
PE20121504A1 (es) 2009-08-19 2012-11-05 Dupont Nutrition Biosci Aps Variantes de glucoamilasa
CN102712915B (zh) 2009-08-19 2014-12-10 丹尼斯科美国公司 具有改良比活和/或热稳定性的葡糖淀粉酶的组合变体
US20120045812A1 (en) 2010-08-06 2012-02-23 Danisco Us Inc. PRODUCTION OF ISOPRENE UNDER NEUTRAL pH CONDITIONS
US20120034659A1 (en) * 2010-08-06 2012-02-09 Danisco Us Inc. NEUTRAL pH SACCHARIFICATION AND FERMENTATION
EP2608682B1 (en) 2010-08-24 2017-12-13 Danisco US Inc. Method of making a snack bar comprising a low temperature rice protein concentrate
CN103732756A (zh) 2011-04-29 2014-04-16 丹尼斯科美国公司 用于高密度葡萄糖浆的淀粉液化和糖化的单pH工艺
US8951764B2 (en) 2011-08-05 2015-02-10 Danisco Us Inc. Production of isoprenoids under neutral pH conditions
CA2849401A1 (en) 2011-09-29 2013-04-04 Danisco Us Inc. Liquefaction and saccharification of granular starch at high concentration
HUE034903T2 (hu) 2012-08-14 2018-03-28 Danisco Us Inc Oxidációval kapcsolatos aktivitásvesztéssel szemben rezisztens trichoderma reesei glükoamiláz változatok és ezek felhasználása
MX2015002099A (es) 2012-08-22 2015-05-11 Dupont Nutrition Biosci Aps Variantes que tienen actividad glucoamilasa.
EP3077507B1 (en) 2013-12-04 2019-07-31 Danisco US Inc. Glucoamylase variants
US9988657B2 (en) 2014-02-27 2018-06-05 E I Du Pont De Nemours And Company Enzymatic hydrolysis of disaccharides and oligosaccharides using alpha-glucosidase enzymes
CN107429267B (zh) 2014-12-19 2021-07-16 丹尼斯科美国公司 葡糖淀粉酶共混物
WO2016138315A1 (en) 2015-02-25 2016-09-01 Danisco Us Inc Alpha-glucosidase, compositions & methods
WO2017205337A1 (en) 2016-05-23 2017-11-30 Dupont Nutrition Biosciences Aps Baking process and a method thereof
WO2018093285A1 (en) 2016-11-18 2018-05-24 Baltika Breweries - Part Of The Carlsberg Group Method of producing a grain malt and the malt product obtained in this way
EP3673039A1 (en) 2017-09-29 2020-07-01 DuPont Nutrition Biosciences ApS Production of brewer's wort having increased fermentable sugars
CN111971379A (zh) * 2018-02-28 2020-11-20 嘉吉公司 葡糖淀粉酶工程化酵母和发酵方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL61982A (en) * 1980-02-15 1984-01-31 Cpc International Inc Genetically engineered microorganisms for massive production of amyloytic enzymes and process for preparing same using the corresponding recombinant dnas containing amylase coding genes
NO840200L (no) * 1983-01-28 1984-07-30 Cefus Corp Glukoamylase cdna.

Also Published As

Publication number Publication date
EP0186066A1 (en) 1986-07-02
DE3587168T2 (de) 1993-09-09
CA1267372A (en) 1990-04-03
JPS61141888A (ja) 1986-06-28
BR8506273A (pt) 1986-08-26
DE3587168D1 (de) 1993-04-15
EP0186066B1 (en) 1993-03-10
ATE86663T1 (de) 1993-03-15
JPH0630586B2 (ja) 1994-04-27
US4863864A (en) 1989-09-05

Similar Documents

Publication Publication Date Title
MX165920B (es) Un procedimiento para producir glucoamilasa
BR8506271A (pt) Processo para produzir etanol
ATE43634T1 (de) Maltose erzeugendes amylase-enzymprodukt, dessen herstellung und dessen verwendung.
DK0778348T3 (da) Fremgangsmåde til fremstilling af et protein ved anvendelse af en svamp transformeret ved multikopi-integration af en ekspr
ES518348A0 (es) Un plasmido vector recombinante.
FI854145A0 (fi) Pichia-sukuisten hiivojen transformointi
ES8106759A1 (es) Un metodo para producir acido l-glutamico por fermentacion
ES360055A1 (es) Un procedimiento para obtener preparados en enzimas proteo-liticas.
ES518157A0 (es) Un procedimiento para preparar una enzima alfa-amilasa termoestable.
ES8602116A1 (es) Un procedimiento para hidrolizar almidon con una enzima de alfa-amilasa
ES8306791A1 (es) Procedimiento para la preparacion de acido acetico.
IT7922818A0 (it) Procedimento per mantenere attiva la cultura di fermenti.
DK0418835T3 (da) Hidtil ukendt alkalisk pullulanase Y med alfa-amylaseaktivitet samt mikroorganisme og fremgangsmåde til fremstilling af samme
ATE21117T1 (de) Gaerungsverfahren zur herstellung von alphaisopropylapfels|ure.
DE3061722D1 (en) Process for producing sphingomyelinase, sphingomyelinase and the use thereof
ES477271A1 (es) Procedimiento para la produccion o el mantenimiento de un cultivo estable de un microorganismo del genero lactovaci- llus.
ES431071A1 (es) Un metodo para producir acido l-glutamico.
JPS5642589A (en) Highly thermophile fumarase, its production and production of l-malic acid utilizing microorganism producing thermophile fumarase and the same
SU1135754A1 (ru) Способ восстановлени серологически атипичных штаммов микроорганизмов в @ -форму
KR880001824A (ko) 미생물에 의한 5'-이노신산의 제조방법
JPS5299282A (en) Preparation of yeast cells
JPS55114288A (en) Production of cholesterol esterase
JPS52139786A (en) Microbial production of uracil, uracilriboside, and uracilribotide
ES8104410A1 (es) Un metodo de produccion de 3-o-desmetil-fortimicina b a par-tir de fortimicina an
JPS52145581A (en) Additives for cultivation medium