PE20121504A1 - Variantes de glucoamilasa - Google Patents

Variantes de glucoamilasa

Info

Publication number
PE20121504A1
PE20121504A1 PE2012000228A PE2012000228A PE20121504A1 PE 20121504 A1 PE20121504 A1 PE 20121504A1 PE 2012000228 A PE2012000228 A PE 2012000228A PE 2012000228 A PE2012000228 A PE 2012000228A PE 20121504 A1 PE20121504 A1 PE 20121504A1
Authority
PE
Peru
Prior art keywords
seq
sequence
amino acids
variant
loop
Prior art date
Application number
PE2012000228A
Other languages
English (en)
Inventor
Peter Edvard Degn
Casper Willem Vroemen
Martijn Silvan Scheffers
Richard Bott
Wolfgang Aehle
Original Assignee
Dupont Nutrition Biosci Aps
Danisco Us Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dupont Nutrition Biosci Aps, Danisco Us Inc filed Critical Dupont Nutrition Biosci Aps
Publication of PE20121504A1 publication Critical patent/PE20121504A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/30—Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
    • A23L29/35—Degradation products of starch, e.g. hydrolysates, dextrins; Enzymatically modified starches
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C5/00—Other raw materials for the preparation of beer
    • C12C5/004—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C7/00—Preparation of wort
    • C12C7/04—Preparation or treatment of the mash
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/20—Preparation of compounds containing saccharide radicals produced by the action of an exo-1,4 alpha-glucosidase, e.g. dextrose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C13—SUGAR INDUSTRY
    • C13K—SACCHARIDES OBTAINED FROM NATURAL SOURCES OR BY HYDROLYSIS OF NATURALLY OCCURRING DISACCHARIDES, OLIGOSACCHARIDES OR POLYSACCHARIDES
    • C13K1/00—Glucose; Glucose-containing syrups
    • C13K1/06—Glucose; Glucose-containing syrups obtained by saccharification of starch or raw materials containing starch
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Food Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Emergency Medicine (AREA)
  • Nutrition Science (AREA)
  • Polymers & Plastics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

SE REFIERE A UNA VARIANTE DE GLUCOAMILASA QUE COMPRENDE DOS O MAS SUSTITUCIONES DE AMINOACIDOS CON RELACION A UN BUCLE INTERCONECTOR 2´ QUE COMPRENDE UNA SECUENCIA DE AMINOACIDOS DESDE LA POSICION 518 A 543 DE LA SEQ ID Nº 2, Y/O A UN BUCLE 1 QUE COMPRENDE LA SECUENCIA DE AMINOACIDOS DESDE LA POSICION 21 A 51 DE LA SEQ ID Nº 2 Y/O A UNA HELICE 2 QUE COMPRENDE UNA SECUENCIA DE AMINOACIDOS DESDE LA POSICION 52 A 68 DE LA SEQ ID Nº 2, Y/O A UN BUCLE 11 QUE COMPRENDE UNA SECUENCIA DE AMINOACIDOS DESDE LA POSICION 396 A 420 DE LA SEQ ID Nº 2, Y/O A UNA HELICE 12 QUE COMPRENDE UNA SECUENCIA DE AMINOACIDOS DESDE LA POSICION 421 A 434 DE LA SEQ ID Nº 2. DICHA VARIANTE TIENE AL MENOS UN 85%, 90%, 95%, 98% O 99.5% DE IDENTIDAD DE SECUENCIA CON LA SEQ ID Nº 2. SE REFIERE TAMBIEN A UN POLINUCLEOTIDO, UN VECTOR, UNA CELULA HUESPED, UN METODO PARA EXPRESAR UNA VARIANTE DE GLUCOAMILASA Y A UNA COMPOSICION ENZIMATICA. DICHA VARIANTE REDUCE LA SINTESIS DE PRODUCTOS DE CONDENSACION DURANTE LA HIDROLISIS DE ALMIDON, SIENDO UTIL PARA LA PRODUCCION DE JARABE DE GLUCOSA
PE2012000228A 2009-08-19 2010-08-18 Variantes de glucoamilasa PE20121504A1 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US23514009P 2009-08-19 2009-08-19
US36089110P 2010-07-01 2010-07-01
DKPA201070337 2010-07-15

Publications (1)

Publication Number Publication Date
PE20121504A1 true PE20121504A1 (es) 2012-11-05

Family

ID=42797171

Family Applications (1)

Application Number Title Priority Date Filing Date
PE2012000228A PE20121504A1 (es) 2009-08-19 2010-08-18 Variantes de glucoamilasa

Country Status (12)

Country Link
US (2) US8809023B2 (es)
EP (1) EP2467474A1 (es)
JP (1) JP5827227B2 (es)
CN (4) CN102575239B (es)
AR (1) AR077942A1 (es)
AU (1) AU2010284963C1 (es)
BR (1) BR112012003787A2 (es)
CA (1) CA2770607C (es)
EA (1) EA023194B1 (es)
MX (1) MX2012002032A (es)
PE (1) PE20121504A1 (es)
WO (2) WO2011020852A1 (es)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112012003787A2 (pt) * 2009-08-19 2017-07-04 Danisco variantes de glicoamilase
CN109321391A (zh) * 2011-04-15 2019-02-12 诺维信公司 生产啤酒麦汁的方法
CN104011067B (zh) * 2011-12-22 2017-12-26 杜邦营养生物科学有限公司 具有葡糖淀粉酶活性的多肽及其制备方法
WO2013148663A1 (en) 2012-03-28 2013-10-03 Danisco Us Inc. Low temperature method for making high glucose syrup
US9540669B2 (en) * 2012-08-14 2017-01-10 Danisco Us Inc. Trichoderma reesei glucoamylase variants resistant to oxidation-related activity loss and the use thereof
MX2015002099A (es) * 2012-08-22 2015-05-11 Dupont Nutrition Biosci Aps Variantes que tienen actividad glucoamilasa.
CN104718289A (zh) * 2012-10-17 2015-06-17 诺维信公司 用于生产啤酒麦芽汁的方法
WO2015017256A1 (en) * 2013-07-29 2015-02-05 Danisco Us Inc. Variant enzymes
BR112016018075B1 (pt) 2014-02-07 2022-01-18 Novozymes A/S Composição compreendendo uma alfa-amilase, uma pululanase e uma enzima glicoamilase e método de fabricação de xarope de glicose a partir de amido liquefeito
US9982284B2 (en) 2014-02-27 2018-05-29 E I Du Pont De Nemours And Company Enzymatic hydrolysis of disaccharides and oligosaccharides using alpha-glucosidase enzymes
EP3227452A1 (en) 2014-12-01 2017-10-11 Novozymes A/S Improved production of glucose syrups
CN113403357A (zh) * 2014-12-19 2021-09-17 丹尼斯科美国公司 葡糖淀粉酶共混物
EP3253779A1 (en) 2015-02-06 2017-12-13 Cargill, Incorporated Modified glucoamylase enzymes and yeast strains having enhanced bioproduct production
CA2979410A1 (en) 2015-03-27 2016-10-06 Cargill, Incorporated Glucoamylase-modified yeast strains and methods for bioproduct production
US9670472B1 (en) 2016-08-05 2017-06-06 Fornia Biosolutions, Inc 2G16 glucoamylase compositions and methods
US10053678B2 (en) 2016-08-05 2018-08-21 Fornia Biosolutions, Inc. G16 glucoamylase compositions and methods
US9598680B1 (en) * 2016-08-05 2017-03-21 Fornia Biosolutions, Inc. G16 glucoamylase compositions and methods
EP3494129A1 (en) 2016-08-05 2019-06-12 Cargill, Incorporated Leader-modified glucoamylase polypeptides and engineered yeast strains having enhanced bioproduct production
US10227578B2 (en) 2016-08-05 2019-03-12 Fornia Biosolutions, Inc. 2G16 glucoamylase compositions and methods
RU2733294C1 (ru) 2016-11-18 2020-10-01 Балтика Брюэрис - Парт Оф Зе Карлсберг Груп Способ получения зернового солода и солодовый продукт, полученный таким способом
EP3759209A4 (en) 2018-02-28 2022-02-09 Cargill, Incorporated GLUCOAMYLASE MANIPULATED YEAST AND FERMENTATION PROCESSES
CN112218953A (zh) * 2018-03-09 2021-01-12 丹尼斯科美国公司 葡糖淀粉酶及其使用方法
WO2020131252A1 (en) 2018-12-21 2020-06-25 Fornia Biosolutions, Inc. Variant g6p g7p glucoamylase compositions and methods
CN114457058B (zh) * 2021-10-19 2024-05-14 安徽农业大学 一种饲用α淀粉酶的突变改良方法及应用
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
WO2024137350A2 (en) 2022-12-19 2024-06-27 Danisco Us Inc. Recombinant fungal strains and methods thereof for producing consistent proteins

Family Cites Families (56)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5534046A (en) 1978-09-01 1980-03-10 Cpc International Inc Novel glucoamyrase having excellent heat resistance and production
GB2089836B (en) 1980-12-16 1984-06-20 Suntory Ltd Process for producing alcohol by fermentation without cooking
US4794175A (en) 1983-12-20 1988-12-27 Cetus Corporation Glucoamylase CDNA
US4618579A (en) 1984-09-28 1986-10-21 Genencor, Inc. Raw starch saccharification
JPH0630586B2 (ja) 1984-12-15 1994-04-27 サントリー株式会社 グルコアミラ−ゼ遺伝子
EP0625577A1 (en) 1985-08-29 1994-11-23 Genencor International, Inc. Heterologous polypeptides expressed in filamentous fungi, processes for their preparation, and vectors for their preparation
US5024941A (en) 1985-12-18 1991-06-18 Biotechnica International, Inc. Expression and secretion vector for yeast containing a glucoamylase signal sequence
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
GB8610600D0 (en) 1986-04-30 1986-06-04 Novo Industri As Transformation of trichoderma
JPS62272987A (ja) 1986-05-22 1987-11-27 Hitachi Plant Eng & Constr Co Ltd ブドウ糖の製造方法
ATE125815T1 (de) 1987-06-06 1995-08-15 Omnigene Inc Expressionsvektor für hefe.
JPH01191693A (ja) 1988-01-25 1989-08-01 Tdk Corp デンプンの連続的糖化方法
US6803499B1 (en) 1989-08-09 2004-10-12 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
JP2733330B2 (ja) 1989-08-31 1998-03-30 日本食品化工株式会社 高純度澱粉糖の製造方法
US6777589B1 (en) 1990-01-22 2004-08-17 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
CA2038485A1 (en) 1990-03-23 1991-09-24 Donald K. Hadden Nanofiltration process for making dextrose
US5162210A (en) * 1990-06-29 1992-11-10 Iowa State University Research Foundation Process for enzymatic hydrolysis of starch to glucose
ES2170748T3 (es) 1990-10-05 2002-08-16 Genencor Int Procedimientos para el tratamiento de tejidos que contienen algodon con celulasa.
US5246853A (en) 1990-10-05 1993-09-21 Genencor International, Inc. Method for treating cotton-containing fabric with a cellulase composition containing endoglucanase components and which composition is free of exo-cellobiohydrolase I
US5475101A (en) 1990-10-05 1995-12-12 Genencor International, Inc. DNA sequence encoding endoglucanase III cellulase
US5290474A (en) 1990-10-05 1994-03-01 Genencor International, Inc. Detergent composition for treating cotton-containing fabrics containing a surfactant and a cellulase composition containing endolucanase III from trichoderma ssp
ES2182818T5 (es) 1990-12-10 2015-07-06 Danisco Us Inc. Sacarificación mejorada de celulosa por clonación y amplificación del gen de beta-glucosidasa de Trichoderma reesei
US5861271A (en) 1993-12-17 1999-01-19 Fowler; Timothy Cellulase enzymes and systems for their expressions
EP0777737B1 (en) 1994-06-30 2005-05-04 Novozymes Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein
US5744716A (en) 1995-06-08 1998-04-28 Scp Global Technologies, A Division Of Preco, Inc. Fluid displacement level, density and concentration measurement system
EP0970193A1 (en) * 1996-07-24 2000-01-12 Iowa State University Research Foundation, Inc. PROTEIN ENGINEERING OF GLUCOAMYLASE TO INCREASE pH OPTIMUM, SUBSTRATE SPECIFICITY AND THERMOSTABILITY
US6255084B1 (en) 1997-11-26 2001-07-03 Novozymes A/S Thermostable glucoamylase
WO2000004136A1 (en) * 1998-07-15 2000-01-27 Novozymes A/S Glucoamylase variants
EP1914306A3 (en) * 1998-07-15 2008-09-10 Novozymes A/S Glucoamylase Variants
US6352851B1 (en) 1998-07-15 2002-03-05 Novozymes A/S Glucoamylase variants
US6268328B1 (en) 1998-12-18 2001-07-31 Genencor International, Inc. Variant EGIII-like cellulase compositions
US6254914B1 (en) 1999-07-02 2001-07-03 The Board Of Trustees Of The University Of Illinois Process for recovery of corn coarse fiber (pericarp)
EP1200566A2 (en) * 1999-07-09 2002-05-02 Novozymes A/S Glucoamylase variant
EP1309677B2 (en) 2000-08-11 2012-04-11 Genencor International, Inc. Bacillus transformation, transformants and mutant libraries
US6582914B1 (en) 2000-10-26 2003-06-24 Genencor International, Inc. Method for generating a library of oligonucleotides comprising a controlled distribution of mutations
AR036370A1 (es) 2001-08-27 2004-09-01 Syngenta Participations Ag Plantas con auto-procesamiento y partes de las mismas
GB0129864D0 (en) 2001-12-13 2002-02-06 Danisco Animal feed
RU2005105054A (ru) * 2002-07-25 2005-08-27 Новозимс А/С (Dk) Способ производства сусла
WO2004081193A2 (en) 2003-03-10 2004-09-23 Broin And Associates, Inc. Method for producing ethanol using raw starch
ES2245621T3 (es) 2003-03-10 2010-05-19 Novozymes A/S Procesos para un producto alcoholico.
EP1862540B1 (en) 2003-05-29 2011-09-14 Genencor International, Inc. Novel trichoderma genes
US6899910B2 (en) 2003-06-12 2005-05-31 The United States Of America As Represented By The Secretary Of Agriculture Processes for recovery of corn germ and optionally corn coarse fiber (pericarp)
CN1875099B (zh) 2003-11-21 2011-05-04 金克克国际有限公司 颗粒淀粉水解酶在木霉菌中的表达和从颗粒淀粉底物产生葡萄糖的方法
US7037704B2 (en) 2004-05-27 2006-05-02 Genencor International, Inc. Heterologous expression of an Aspergillus kawachi acid-stable alpha amylase and applications in granular starch hydrolysis
US7413887B2 (en) 2004-05-27 2008-08-19 Genecor International, Inc. Trichoderma reesei glucoamylase and homologs thereof
GB0423139D0 (en) 2004-10-18 2004-11-17 Danisco Enzymes
DK1824970T3 (da) 2004-11-30 2011-10-31 Genencor Int Thricoderma reesei-glycoamylase og homologer heraf
CN101094918B (zh) 2004-12-30 2014-05-14 金克克国际有限公司 酸性真菌蛋白酶
US20080280328A1 (en) * 2005-11-18 2008-11-13 Novozymes A/S Glucoamylase Variants
US7608436B2 (en) * 2006-01-25 2009-10-27 Tate & Lyle Ingredients Americas, Inc. Process for producing saccharide oligomers
CA2665595A1 (en) 2006-10-10 2008-04-17 Danisco Us, Inc., Genencor Division Glucoamylase variants with altered properties
BRPI0719449A2 (pt) * 2006-12-21 2017-06-20 Syngenta Participations Ag molécula de ácido nucléico isolada, sintética ou recombinante, polipeptídeo isolado, planta ou parte de planta, eículo de clonagem que compreende um ácido nucléico, cassete de expressão que compreende uma molécula de ácido nucléico e método para hidrolisar um polissacarídeo, oligossacarídeo ou amido
EP2481796B1 (en) 2007-10-09 2014-06-11 Danisco US Inc. Glucoamylase variants
MX2010005461A (es) * 2007-11-20 2010-06-02 Danisco Us Inc Variantes de glucoamilasa con propiedades alteradas.
BR112012003787A2 (pt) * 2009-08-19 2017-07-04 Danisco variantes de glicoamilase
CN102712915B (zh) 2009-08-19 2014-12-10 丹尼斯科美国公司 具有改良比活和/或热稳定性的葡糖淀粉酶的组合变体

Also Published As

Publication number Publication date
MX2012002032A (es) 2012-03-26
CA2770607A1 (en) 2011-02-24
EA201270286A1 (ru) 2013-02-28
CA2770607C (en) 2019-02-26
JP5827227B2 (ja) 2015-12-02
CN102575239B (zh) 2017-06-16
US20120149070A1 (en) 2012-06-14
AR077942A1 (es) 2011-10-05
US20130102035A1 (en) 2013-04-25
AU2010284963C1 (en) 2014-11-27
EA023194B1 (ru) 2016-05-31
AU2010284963B2 (en) 2014-03-20
US20130309726A9 (en) 2013-11-21
CN102575239A (zh) 2012-07-11
JP2013502211A (ja) 2013-01-24
CN109295032A (zh) 2019-02-01
CN107299123A (zh) 2017-10-27
WO2011020852A1 (en) 2011-02-24
US9469848B2 (en) 2016-10-18
CN103119157B (zh) 2018-10-09
EP2467474A1 (en) 2012-06-27
CN103119157A (zh) 2013-05-22
BR112012003787A2 (pt) 2017-07-04
WO2012001139A1 (en) 2012-01-05
AU2010284963A1 (en) 2012-03-08
US8809023B2 (en) 2014-08-19
WO2012001139A9 (en) 2012-02-23

Similar Documents

Publication Publication Date Title
PE20121504A1 (es) Variantes de glucoamilasa
Jansen et al. Towards large scale fermentative production of succinic acid
WO2007138256A3 (en) Conversion method
WO2010080408A3 (en) Methods for increasing enzymatic hydrolysis of cellulosic material in the presence of a peroxidase
MY170602A (en) Process for fractionation of biomass
MX2015016379A (es) Produccion de diterpeno extracelular.
AR069167A1 (es) Variantes de alfa- amilasa de bacillus licheniformis con termoestabilidad aumentada y/o dependencia de calcio disminuida
BR112014014640A2 (pt) método para prover um sistema de conversão de biomassa, e, sistema de conversão de biomassa
IN2012DN01652A (es)
WO2008153815A3 (en) Variants of an alpha-amylase with improved production levels in fermentation processes
WO2011142865A3 (en) Yeast microorganisms with reduced by-product accumulation for improved production of fuels, chemicals, and amino acids
IN2012DN02509A (es)
WO2014018368A3 (en) Methods for increasing enzymatic hydrolysis of cellulosic material
NZ628964A (en) Supercritical hydrolysis of biomass
EP2481479A4 (en) A CATALYST FOR THE HYDROLYSIS OF CELLULOSE OR HEMIZELLULOSE AND A METHOD FOR THE PRODUCTION OF A SUGAR-CONTAINING SOLUTION THROUGH THE CATALYST
WO2009049284A3 (en) Compositions and methods for improved glycoprotein sialylation
AR071969A1 (es) Proteasa especifica de prolina identificada en el hongo penicillium chrysogenum
WO2011050434A3 (en) Enhanced process to produce a thermofertiliser from potassium-bearing minerals
WO2009015663A3 (de) Trocknungsverfahren innerhalb der sauren hydrolyse von pflanzlichen biomassen
BRPI0910812A2 (pt) célula de fermentação de açúcar pentose
BR112014018028A8 (pt) Processo para preparação de sílicas precipitadas
BR112012033701A2 (pt) polipeptídeo tendo atividade de degradação de carbohidrato e seus usos
BR112015014201A8 (pt) processo para hidrólise enzimática de celulose, celulose hidrolisada enzimaticamente, e uso de extrato aquoso
WO2014057437A3 (fr) Procede de preparation de proteine c1q recombinante
WO2012134253A3 (en) Corynebacterium sp. transformed with a fructokinase gene derived from escherichia sp. and process for preparing l-amino acid using the same

Legal Events

Date Code Title Description
FC Refusal