FR2599755B1 - Bloc d'expression d'une amyloglucosidase dans une levure, levure transformee et procede de preparation d'enzyme - Google Patents

Bloc d'expression d'une amyloglucosidase dans une levure, levure transformee et procede de preparation d'enzyme

Info

Publication number
FR2599755B1
FR2599755B1 FR8608387A FR8608387A FR2599755B1 FR 2599755 B1 FR2599755 B1 FR 2599755B1 FR 8608387 A FR8608387 A FR 8608387A FR 8608387 A FR8608387 A FR 8608387A FR 2599755 B1 FR2599755 B1 FR 2599755B1
Authority
FR
France
Prior art keywords
yeast
amyloglucosidase
enzyme
preparation
expression block
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
FR8608387A
Other languages
English (en)
Other versions
FR2599755A1 (fr
Inventor
Nathalie Labat
Martine Nguyen-Juilleret
Gerard Loison
Yves Lemoine
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Transgene SA
Original Assignee
Transgene SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Transgene SA filed Critical Transgene SA
Priority to FR8608387A priority Critical patent/FR2599755B1/fr
Priority to FR878705208A priority patent/FR2613727B2/fr
Priority to FR878705207A priority patent/FR2613726B2/fr
Priority to DK293787A priority patent/DK293787A/da
Priority to EP87401300A priority patent/EP0260160A1/fr
Priority to JP62144992A priority patent/JPS63102685A/ja
Priority to AU74435/87A priority patent/AU612112C/en
Publication of FR2599755A1 publication Critical patent/FR2599755A1/fr
Application granted granted Critical
Publication of FR2599755B1 publication Critical patent/FR2599755B1/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
FR8608387A 1986-06-10 1986-06-10 Bloc d'expression d'une amyloglucosidase dans une levure, levure transformee et procede de preparation d'enzyme Expired - Fee Related FR2599755B1 (fr)

Priority Applications (7)

Application Number Priority Date Filing Date Title
FR8608387A FR2599755B1 (fr) 1986-06-10 1986-06-10 Bloc d'expression d'une amyloglucosidase dans une levure, levure transformee et procede de preparation d'enzyme
FR878705208A FR2613727B2 (fr) 1986-06-10 1987-04-13 Integration dans la souche polyploide saccharomyces uvarum 2285 tg2 amgc du bloc d'expression de l'alpha-amylase de b. licheniformis
FR878705207A FR2613726B2 (fr) 1986-06-10 1987-04-13 Integration dans la souche polyploide de boulangerie (tgf1) du bloc d'expression de l'alpha-amyloglucosidase de aspergillus niger
DK293787A DK293787A (da) 1986-06-10 1987-06-09 Ekspression af en amyloglucosidase
EP87401300A EP0260160A1 (fr) 1986-06-10 1987-06-10 Bloc d'expression d'une amyloglucosidase dans une levure ainsi que les levures le contenant
JP62144992A JPS63102685A (ja) 1986-06-10 1987-06-10 酵母中のアミログルコシダ−ゼ発現ブロック、形質転換された酵母、酵素の製造方法および発酵法
AU74435/87A AU612112C (en) 1986-06-10 1987-06-15 Block for the expression of an aminolucosidase in yeast, transformed yeast, enzyme preparation process and fermentation process

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR8608387A FR2599755B1 (fr) 1986-06-10 1986-06-10 Bloc d'expression d'une amyloglucosidase dans une levure, levure transformee et procede de preparation d'enzyme

Publications (2)

Publication Number Publication Date
FR2599755A1 FR2599755A1 (fr) 1987-12-11
FR2599755B1 true FR2599755B1 (fr) 1990-04-20

Family

ID=9336201

Family Applications (1)

Application Number Title Priority Date Filing Date
FR8608387A Expired - Fee Related FR2599755B1 (fr) 1986-06-10 1986-06-10 Bloc d'expression d'une amyloglucosidase dans une levure, levure transformee et procede de preparation d'enzyme

Country Status (2)

Country Link
JP (1) JPS63102685A (fr)
FR (1) FR2599755B1 (fr)

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NO840200L (no) * 1983-01-28 1984-07-30 Cefus Corp Glukoamylase cdna.
WO1984004330A1 (fr) * 1983-04-22 1984-11-08 Amgen Secretion de polypeptides exogenes a partir de levure
EP0143821A1 (fr) * 1983-05-19 1985-06-12 Transgene S.A. Production de la catechol 2,3-oxygenase par des levures, plasmide pour sa mise en oeuvre et application
IL75210A0 (en) * 1984-05-22 1985-09-29 Bioteknika International Yeast vector
JPH0630586B2 (ja) * 1984-12-15 1994-04-27 サントリー株式会社 グルコアミラ−ゼ遺伝子
JPS61141890A (ja) * 1984-12-15 1986-06-28 Suntory Ltd アルコ−ルの製造法
GB8432483D0 (en) * 1984-12-21 1985-02-06 Tubb R S Enzymes
AU5954486A (en) * 1985-05-21 1986-12-24 Biotechnica International Inc. Yeast expressing gluocoamylase

Also Published As

Publication number Publication date
FR2599755A1 (fr) 1987-12-11
JPS63102685A (ja) 1988-05-07

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Legal Events

Date Code Title Description
ST Notification of lapse