FR2613726B2 - Integration dans la souche polyploide de boulangerie (tgf1) du bloc d'expression de l'alpha-amyloglucosidase de aspergillus niger - Google Patents

Integration dans la souche polyploide de boulangerie (tgf1) du bloc d'expression de l'alpha-amyloglucosidase de aspergillus niger

Info

Publication number
FR2613726B2
FR2613726B2 FR878705207A FR8705207A FR2613726B2 FR 2613726 B2 FR2613726 B2 FR 2613726B2 FR 878705207 A FR878705207 A FR 878705207A FR 8705207 A FR8705207 A FR 8705207A FR 2613726 B2 FR2613726 B2 FR 2613726B2
Authority
FR
France
Prior art keywords
tgf1
polyploid
amyloglucosidase
alpha
integration
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
FR878705207A
Other languages
English (en)
Other versions
FR2613726A2 (fr
Inventor
Nathalie Labat
Martine Nguyen-Juilleret
Gerard Loison
Yves Lemoine
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Transgene SA
Original Assignee
Transgene SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from FR8608387A external-priority patent/FR2599755B1/fr
Application filed by Transgene SA filed Critical Transgene SA
Priority to FR878705207A priority Critical patent/FR2613726B2/fr
Priority to DK293787A priority patent/DK293787A/da
Priority to EP87401300A priority patent/EP0260160A1/fr
Priority to AU74435/87A priority patent/AU612112C/en
Publication of FR2613726A2 publication Critical patent/FR2613726A2/fr
Application granted granted Critical
Publication of FR2613726B2 publication Critical patent/FR2613726B2/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • C12N9/2414Alpha-amylase (3.2.1.1.)
    • C12N9/2417Alpha-amylase (3.2.1.1.) from microbiological source
    • AHUMAN NECESSITIES
    • A21BAKING; EDIBLE DOUGHS
    • A21DTREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
    • A21D8/00Methods for preparing or baking dough
    • A21D8/02Methods for preparing dough; Treating dough prior to baking
    • A21D8/04Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes
    • A21D8/047Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes with yeasts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/16Yeasts; Culture media therefor
    • C12N1/18Baker's yeast; Brewer's yeast
    • C12N1/185Saccharomyces isolates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • C12N9/2428Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/645Fungi ; Processes using fungi
    • C12R2001/85Saccharomyces
    • C12R2001/865Saccharomyces cerevisiae

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Mycology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)
FR878705207A 1986-06-10 1987-04-13 Integration dans la souche polyploide de boulangerie (tgf1) du bloc d'expression de l'alpha-amyloglucosidase de aspergillus niger Expired - Lifetime FR2613726B2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
FR878705207A FR2613726B2 (fr) 1986-06-10 1987-04-13 Integration dans la souche polyploide de boulangerie (tgf1) du bloc d'expression de l'alpha-amyloglucosidase de aspergillus niger
DK293787A DK293787A (da) 1986-06-10 1987-06-09 Ekspression af en amyloglucosidase
EP87401300A EP0260160A1 (fr) 1986-06-10 1987-06-10 Bloc d'expression d'une amyloglucosidase dans une levure ainsi que les levures le contenant
AU74435/87A AU612112C (en) 1986-06-10 1987-06-15 Block for the expression of an aminolucosidase in yeast, transformed yeast, enzyme preparation process and fermentation process

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR8608387A FR2599755B1 (fr) 1986-06-10 1986-06-10 Bloc d'expression d'une amyloglucosidase dans une levure, levure transformee et procede de preparation d'enzyme
FR878705207A FR2613726B2 (fr) 1986-06-10 1987-04-13 Integration dans la souche polyploide de boulangerie (tgf1) du bloc d'expression de l'alpha-amyloglucosidase de aspergillus niger

Publications (2)

Publication Number Publication Date
FR2613726A2 FR2613726A2 (fr) 1988-10-14
FR2613726B2 true FR2613726B2 (fr) 1990-07-20

Family

ID=26225302

Family Applications (1)

Application Number Title Priority Date Filing Date
FR878705207A Expired - Lifetime FR2613726B2 (fr) 1986-06-10 1987-04-13 Integration dans la souche polyploide de boulangerie (tgf1) du bloc d'expression de l'alpha-amyloglucosidase de aspergillus niger

Country Status (1)

Country Link
FR (1) FR2613726B2 (fr)

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS52130977A (en) * 1976-04-22 1977-11-02 Oriental Yeast Co Ltd Preparation of baker.s yeast with excellent flavor
NO840200L (no) * 1983-01-28 1984-07-30 Cefus Corp Glukoamylase cdna.
IL75210A0 (en) * 1984-05-22 1985-09-29 Bioteknika International Yeast vector
GB8432483D0 (en) * 1984-12-21 1985-02-06 Tubb R S Enzymes
WO1986007091A1 (fr) * 1985-05-21 1986-12-04 Biotechnica International, Inc. Levure exprimant de la glucoamylase
EP0257115A1 (fr) * 1986-08-21 1988-03-02 Heineken Technisch Beheer B.V. Micro-organismes produisant des enzymes amylolytiques et construits par la technologie des ADN recombinants et leur utilisation dans des procédés de fermentation

Also Published As

Publication number Publication date
FR2613726A2 (fr) 1988-10-14

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