FR2613727B2 - Integration dans la souche polyploide saccharomyces uvarum 2285 tg2 amgc du bloc d'expression de l'alpha-amylase de b. licheniformis - Google Patents

Integration dans la souche polyploide saccharomyces uvarum 2285 tg2 amgc du bloc d'expression de l'alpha-amylase de b. licheniformis

Info

Publication number
FR2613727B2
FR2613727B2 FR878705208A FR8705208A FR2613727B2 FR 2613727 B2 FR2613727 B2 FR 2613727B2 FR 878705208 A FR878705208 A FR 878705208A FR 8705208 A FR8705208 A FR 8705208A FR 2613727 B2 FR2613727 B2 FR 2613727B2
Authority
FR
France
Prior art keywords
amgc
integration
licheniformis alpha
saccharomyces uvarum
expression block
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
FR878705208A
Other languages
English (en)
Other versions
FR2613727A2 (fr
Inventor
Nathalie Labat
Martine Nguyen-Juilleret
Gerard Loison
Yves Lemoine
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Transgene SA
Original Assignee
Transgene SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from FR8608387A external-priority patent/FR2599755B1/fr
Application filed by Transgene SA filed Critical Transgene SA
Priority to FR878705208A priority Critical patent/FR2613727B2/fr
Priority to DK293787A priority patent/DK293787A/da
Priority to EP87401300A priority patent/EP0260160A1/fr
Priority to AU74435/87A priority patent/AU612112C/en
Publication of FR2613727A2 publication Critical patent/FR2613727A2/fr
Application granted granted Critical
Publication of FR2613727B2 publication Critical patent/FR2613727B2/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C12N1/185—Saccharomyces isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/85—Saccharomyces
FR878705208A 1986-06-10 1987-04-13 Integration dans la souche polyploide saccharomyces uvarum 2285 tg2 amgc du bloc d'expression de l'alpha-amylase de b. licheniformis Expired - Lifetime FR2613727B2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
FR878705208A FR2613727B2 (fr) 1986-06-10 1987-04-13 Integration dans la souche polyploide saccharomyces uvarum 2285 tg2 amgc du bloc d'expression de l'alpha-amylase de b. licheniformis
DK293787A DK293787A (da) 1986-06-10 1987-06-09 Ekspression af en amyloglucosidase
EP87401300A EP0260160A1 (fr) 1986-06-10 1987-06-10 Bloc d'expression d'une amyloglucosidase dans une levure ainsi que les levures le contenant
AU74435/87A AU612112C (en) 1986-06-10 1987-06-15 Block for the expression of an aminolucosidase in yeast, transformed yeast, enzyme preparation process and fermentation process

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR8608387A FR2599755B1 (fr) 1986-06-10 1986-06-10 Bloc d'expression d'une amyloglucosidase dans une levure, levure transformee et procede de preparation d'enzyme
FR878705208A FR2613727B2 (fr) 1986-06-10 1987-04-13 Integration dans la souche polyploide saccharomyces uvarum 2285 tg2 amgc du bloc d'expression de l'alpha-amylase de b. licheniformis

Publications (2)

Publication Number Publication Date
FR2613727A2 FR2613727A2 (fr) 1988-10-14
FR2613727B2 true FR2613727B2 (fr) 1990-07-20

Family

ID=26225303

Family Applications (1)

Application Number Title Priority Date Filing Date
FR878705208A Expired - Lifetime FR2613727B2 (fr) 1986-06-10 1987-04-13 Integration dans la souche polyploide saccharomyces uvarum 2285 tg2 amgc du bloc d'expression de l'alpha-amylase de b. licheniformis

Country Status (1)

Country Link
FR (1) FR2613727B2 (fr)

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2533583A1 (fr) * 1982-09-24 1984-03-30 Centre Nat Rech Scient Nouvel adn recombinant utile dans la preparation d'a-amylase
NO840200L (no) * 1983-01-28 1984-07-30 Cefus Corp Glukoamylase cdna.
IL75210A0 (en) * 1984-05-22 1985-09-29 Bioteknika International Yeast vector
GB8432483D0 (en) * 1984-12-21 1985-02-06 Tubb R S Enzymes
WO1986007091A1 (fr) * 1985-05-21 1986-12-04 Biotechnica International, Inc. Levure exprimant de la glucoamylase
EP0257115A1 (fr) * 1986-08-21 1988-03-02 Heineken Technisch Beheer B.V. Micro-organismes produisant des enzymes amylolytiques et construits par la technologie des ADN recombinants et leur utilisation dans des procédés de fermentation

Also Published As

Publication number Publication date
FR2613727A2 (fr) 1988-10-14

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