EA201201449A1 - Способ получения клеток, способных преобразовывать арабинозу - Google Patents

Способ получения клеток, способных преобразовывать арабинозу

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Publication number
EA201201449A1
EA201201449A1 EA201201449A EA201201449A EA201201449A1 EA 201201449 A1 EA201201449 A1 EA 201201449A1 EA 201201449 A EA201201449 A EA 201201449A EA 201201449 A EA201201449 A EA 201201449A EA 201201449 A1 EA201201449 A1 EA 201201449A1
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Eurasian Patent Office
Prior art keywords
cell
arabinose
converting
abovable
constructed
Prior art date
Application number
EA201201449A
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English (en)
Inventor
Пол Классен
Бьянка Элизабет Мария Гелесен
Вилберт Герман Мари Хейне
Гийсбердина Пьетернелла Суйлеком Ван
Original Assignee
ДСМ АйПи АССЕТС Б.В.
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=44833745&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=EA201201449(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by ДСМ АйПи АССЕТС Б.В. filed Critical ДСМ АйПи АССЕТС Б.В.
Publication of EA201201449A1 publication Critical patent/EA201201449A1/ru

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Abstract

Изобретение относится к способу получения клеток, способных преобразовывать арабинозу, который включает следующие стадии: a) введение в штамм-хозяин, который не способен преобразовывать арабинозу, генов AraA, AraB и AraD, при этом эту клетку называют сконструированная клетка; b) применение к сконструированной клетке адаптивной эволюции для получения клетки, преобразующей арабинозу; c) необязательно, применение к первой преобразующей арабинозу клетке адаптивной эволюции для улучшения преобразования арабинозы; клетка, полученная на стадии b) или с), обозначается как первая преобразующая арабинозу клетка; d) анализ полного генома или части генома первой преобразующей арабинозу клетки и сконструированной клетки; e) идентификация однонуклеотидных полиморфизмов (SNP) в первой преобразующей арабинозу клетке; f) использование информации SNP при рациональном дизайне клетки, способной преобразовывать арабинозу; g) конструирование клетки, способной преобразовывать арабинозу, согласно дизайну стадии f).
EA201201449A 2010-04-21 2011-04-19 Способ получения клеток, способных преобразовывать арабинозу EA201201449A1 (ru)

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