BR112018011567A2 - método de purificação de uma proteína de interesse, e, uso de um método. - Google Patents

método de purificação de uma proteína de interesse, e, uso de um método.

Info

Publication number
BR112018011567A2
BR112018011567A2 BR112018011567A BR112018011567A BR112018011567A2 BR 112018011567 A2 BR112018011567 A2 BR 112018011567A2 BR 112018011567 A BR112018011567 A BR 112018011567A BR 112018011567 A BR112018011567 A BR 112018011567A BR 112018011567 A2 BR112018011567 A2 BR 112018011567A2
Authority
BR
Brazil
Prior art keywords
interest
protein
particulate matter
fermentation broth
purification
Prior art date
Application number
BR112018011567A
Other languages
English (en)
Inventor
Schaedler Andreas
Treier-Marxen Katrin
Deckert Petra
Kaeding Thomas
Navickas Vaidotas
Wengert Volker
Original Assignee
Basf Se
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Basf Se filed Critical Basf Se
Publication of BR112018011567A2 publication Critical patent/BR112018011567A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02—Separating microorganisms from their culture media
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/14—Ultrafiltration; Microfiltration
    • B01D61/147—Microfiltration
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/14—Ultrafiltration; Microfiltration
    • B01D61/16—Feed pretreatment
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2311/00—Details relating to membrane separation process operations and control
    • B01D2311/04—Specific process operations in the feed stream; Feed pretreatment
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2311/00—Details relating to membrane separation process operations and control
    • B01D2311/18—Details relating to membrane separation process operations and control pH control
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2315/00—Details relating to the membrane module operation
    • B01D2315/16—Diafiltration

Abstract

a presente invenção é dirigida a um método de purificação de uma proteína de interesse da matéria particulada de um caldo de fermentação compreendendo a etapa de purificação da proteína de interesse da matéria particulada do caldo de fermentação, em que a etapa de purificação da proteína de interesse da matéria particulada do caldo de fermentação compreende uma ou mais etapas de lavagem, que compreendem colocar a matéria particulada em contato com uma solução de lavagem compreendendo uma ou mais condições que favorecem a solubilização da proteína de interesse e/ou a dessorção da proteína de interesse da matéria particulada.
BR112018011567A 2015-12-09 2016-12-08 método de purificação de uma proteína de interesse, e, uso de um método. BR112018011567A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP15198622 2015-12-09
PCT/EP2016/080165 WO2017097869A1 (en) 2015-12-09 2016-12-08 Method of purifying a protein from fermentation solids under desorbing conditions

Publications (1)

Publication Number Publication Date
BR112018011567A2 true BR112018011567A2 (pt) 2018-11-27

Family

ID=55023859

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112018011567A BR112018011567A2 (pt) 2015-12-09 2016-12-08 método de purificação de uma proteína de interesse, e, uso de um método.

Country Status (10)

Country Link
US (1) US20190024038A1 (pt)
EP (2) EP3386612A1 (pt)
JP (1) JP2018536418A (pt)
KR (1) KR20180085737A (pt)
CN (1) CN108367245A (pt)
BR (1) BR112018011567A2 (pt)
CA (1) CA3005292A1 (pt)
MX (1) MX2018007067A (pt)
RU (1) RU2018124771A (pt)
WO (1) WO2017097869A1 (pt)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110506048A (zh) 2017-04-03 2019-11-26 诺维信公司 回收方法
CN114127256A (zh) 2019-02-20 2022-03-01 巴斯夫欧洲公司 用确定成分培养基和微量元素补料的芽孢杆菌工业发酵工艺
WO2020169563A1 (en) 2019-02-20 2020-08-27 Basf Se Industrial fermentation process for bacillus using defined medium and magnesium feed
CN113993878A (zh) * 2019-06-13 2022-01-28 巴斯夫欧洲公司 用二价阳离子从发酵液中回收蛋白的方法
ES2955800T3 (es) * 2019-07-05 2023-12-07 Basf Se Procedimiento de fermentación industrial de células microbianas mediante precultivo alimentado por lotes
WO2024033134A1 (en) 2022-08-11 2024-02-15 Basf Se Enzyme compositions comprising protease, mannanase, and/or cellulase
WO2024033136A1 (en) 2022-08-11 2024-02-15 Basf Se Amylase variants
WO2024033133A2 (en) 2022-08-11 2024-02-15 Basf Se Enzyme compositions comprising an amylase
WO2024033135A2 (en) 2022-08-11 2024-02-15 Basf Se Amylase variants
CN117024567A (zh) * 2023-10-09 2023-11-10 健通(济南)生物科技有限公司 一种重组人血清白蛋白发酵液离心收率提高工艺

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Also Published As

Publication number Publication date
EP3386612A1 (en) 2018-10-17
WO2017097869A1 (en) 2017-06-15
US20190024038A1 (en) 2019-01-24
MX2018007067A (es) 2018-08-01
CN108367245A (zh) 2018-08-03
EP4218992A3 (en) 2023-08-09
CA3005292A1 (en) 2017-06-15
JP2018536418A (ja) 2018-12-13
KR20180085737A (ko) 2018-07-27
RU2018124771A (ru) 2020-01-13
EP4218992A2 (en) 2023-08-02

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B11A Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing
B11Y Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette]